BioLAB BSDBU-201-B User manual
Operation Manual
Double Beam UV Visible Spectrophotometer
BSDBU-201-B
Thank you for Choosing Biolab products. Please read the “Operating Instructions” and
“Warranty” before operating this unit to assure proper operation.
Index
Safety ……………………………………………………………………...........2
General ………………………………………………………………………......... 2
Electrical ……………………………………………………………………..........2
Warning ………………………………………………………………………........3
Performance ........…………………………………………………………..........3
Radio Interference …………………………………………………………........3
Introduction …………………………………………………………………........5
Working Principle …………………………………………………………..........6
npacking Instructions ……………………………………………………........7
Specifications ……………………………………………..…………………........8
Installation ……………………………………………………………………........8
Operation ……………………………………………………..……….…….......9
Prepare the Spectrophotometer …………………………………………..6
Analyse Sample …………………………………………………………….. 17
Setting and Calibration …………………………………………………….. 39
Instrument Maintenance….………………………………………………… 45
Daily Maintenance ……………………………………………………….. 45
Trouble Shooting ……………………………………………………… 45
Spare parts Replacement …………………………………..………… 49
Appendix A ……………………………………………………………… 55
Appendix B ……………………………………………………………… 55
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
1 Safety
The safety statements in this manual comply with the requirements of the HEALTH AND
SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its accessories. This
instrument should be operated by appropriate laboratory technicians.
2 General
The apparatus described in this manual is designed to be used by properly trained personnel
in a suitable equipped laboratory. For the correct and safe use of this apparatus it is essential
that laboratory personnel follow generally accepted safe procedures in addition to the safety
precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the
power supply unit is a hazardous area and its cover should not be removed under any
circumstances. There are no serviceable components inside this power supply unit. For this
instrument, avoid touching the high voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or inflammable and
samples may be radioactive, toxic, or potentially infective. Care should be taken to follow the
normal laboratory procedures for handling chemicals and samples.
3 Electrical
Before switching on the apparatus, make sure it is set to the voltage of the local power supply
(see Installation).
The power cord shall be inserted in a socket provided with a protective earth contact. The
protective action must not be negated by the use of an extension cord without a protective
conductor.
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4 Warning
Any interruption of the protective conductor inside or outside the apparatus or disconnection
of the protective earth terminal is likely to make the apparatus dangerous. Intentional
interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation.
Note：NEVER touch or handle the power supply on this instrument due to the high voltage ！
The protection is likely to be impaired if, for example, the apparatus
• Shows visible damage
• Fails to perform the intended measurements
• Has been subjected to prolonged storage under unfavorable conditions
• Has been subjected to severe transport stresses
5 Performance
To ensure that the instrument is working within its specification, especially when making
measurements of an important nature，carry out performance checks with particular
reference to wavelength and absorbance accuracy. Performance checks are detailed in this
manual.
6 Radio Interference
For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is
necessary that only shielded cables supplied by us are used when connecting the instrument
to computers and accessories.
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
7 Introduction
This instrument (Fig 1) is a double beam, general purpose instrument designed to meet the
needs of the Conventional Laboratory, This instrument is ideal for various applications, such
as: Chemistry, Biochemistry, petrochemistry, Environmental Protection, Food and Beverage
Labs, Water and Waste Water Labs and other fields of quality control and research.
This instrument incorporates a 32 ×24 dot matrix LCD display for photometric results, easy
operation and wavelength range of 19 nm to 11 nm. This instrument is ideal for
measurements in the visible and ultraviolet wavelength region of the electromagnetic
spectrum.
Double Beam UV Visible Spectrophotometer BSDBU-201-B
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The Sample Compartment
8 Working Principle
The spectrophotometer consists of five parts:
1) Halogen or deuterium lamps to supply the light;
2) A Monochromator to isolate the wavelength of interest and eliminate the unwanted second
order radiation;
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
3) A sample compartment to accommodate the sample solution;
4) Two detectors to receive the transmitted light and convert it to an electrical signal; and 5) A
digital display to indicate absorbance or transmittance.
The block diagram (Fig 2) below illustrates the relationship between these parts.
Block diagram for the Spectrophotometer
Fig 2
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The grating
disperses the light beam to produce the spectrum, a portion of which is focused on the exit
slit of the monochromator by a collimating mirror. From here the beam is passed to a sample
compartment through one of the filters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment, the beam is
passed to the silicon photodiode detector and causes the detector to produce an electrical
signal that is displayed on the digital display
9 Unpacking Instructions
Carefully unpack the contents and check the materials against the following packing list to
ensure that you have received everything in good condition.
Packing List
Description Quantity
• Spectrophotometer …………………………………............................ 1
• Mains Lead ……………………………………............................ 1
• Glass Cuvettes …………………………………………..................... 1 Set of 4
• Quartz Cuvettes .............................................................................1 Set of 2
• Dust Cover ……………………………………............................ 1
• Operation Manual …………………………………….......................... 1
• Software Manual ……………………………………............................ 1
• Software Kit (Disc 1+ USB Lead 1+ Dongle 1) ........................1
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Note: The printer and auto-cell holder mentioned in this manual are all optional accessories,
they do not come standard with the instrument.
1 Specifications:
• Wavelength Range: 19 -11 nm
• Spectral Bandpass: .5/1/2/4nm
• Wavelength Accuracy: ± .3nm
• Wavelength Repeatability: .2nm
• Baseline Flatness. ± . 1A
• Stray Light : . 5%@22 nm&36 nm
• Photometric Range: -2 %T,- .3-3. A
• Noise: . 1A @ 5 nm
• Drift: < . 2A/h @ 5 nm
• Power Requirements: AC 11 V/6 Hz or 22 V/5 Hz
• Dimensions(mm) 625L×43 L×21 H
• Light Source: Tungsten Halogen/Deuterium
• Net Weight: 3 kg
11 Installation:
1. After carefully unpacking the contents, check the materials with the packing list (page 4) to
ensure that you have received everything in good condition.
2. Place the instrument in a suitable location away from direct sunlight. In order to have the
best performance from your instrument, keep it as far as possible from any strong magnetic
or electrical fields or any electrical device that may generate high-frequency fields. Set the unit
up in an area that is free of dust, corrosive gases and strong vibrations.
3. Remove any obstructions or materials that could hinder the flow of air under and around
the instrument.
4. Use the appropriate power cord and plug into a grounded outlet.
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
5. Turn on your spectrophotometer. Allow it to warm up for 15 minutes before taking any
readings. We suggest you then do the Calibrate System with the Search 656.1nm to set the
wavelength to the deuterium lamp emission line.
NOTE:
This symbol means Caution, Risk of Danger.
12 Operation:
Prepare the spectrophotometer
Fig 3 is the control panel. User can perform all operations by pressing the keys and all the
results and operation information are displayed on the LCD.
Fig 3
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• Description of keys
【LOAD】 Load data or curve saved before;
【SAVE】 Save data or curve;
【SETλ】 Set wavelength;
【ZERO 】 Blank or scan the user base line;
【PRINT】 Print test results or screen
【START】 Start testing or scanning sample;
【ESC/STOP】 Exit to previous screen or cancel the operation;
【ENTER】 Confirm the inputted data or selected item; Go into next setup or screen;
【F1】-【F4】 Function based on the information on the screen;
【】-【9】 Input number or letter, consecutively press a numeric key to select a character;
【+/-/.】 Input +,- or dot;
【CE】 Clear all characters when you are inputting or clear curve displays on the screen;
【＜】,【＞】 Change “x” scale; Search point after scan; 【＜】 clear a character;
【 】∧,【 】∨Change “y” scale; Search peak after scan; Scroll items for selecting; Change
capital/small letter last typed in; Browse the items for selection;
【CELL】Set cell position (Only available when Auto Changer used).
【HELP 】Reserved key for future Function Extending, not available now.
Note：When the 8-hole Auto Changer used, press the key of 【CELL】first, then press the
Hole No. on the numerical keypad and press the key of【ENTER】to confirm, then the cell you
chose would go to the light path automatically.
• Turn on spectrophotometer
Turn on spectrophotometer by pressing the Power Switch (IO)(see Fig1). The instrument starts
to initiate and the steps are as below:
1.The instrument will check memory first (Fig 4), please wait or press any key to skip this
step ,after positioning filter, auto-cell changer(if installed) and D2/W lamps, the screen display
as Fig 4A. 15 minutes pass or press【ESC】, the screen display as Fig 5,Select “No” to skip to
main menu( Fig 7) and select “Yes”(recommended) to calibrate system (Fig 6).The calibrating
process include “get dark current”, “searching 656.1nm” and “check energy”. After finish the
calibration system, go to main menu too (Fig 7).
2. If the data in memory has been lost, the instrument will directly calibrate system without
any choice for you.
3. If no auto-cell changer installed “cell #1” will disappear in Fig7
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
Fig 6
Fig 7
• Basic operation
Set Slit
The default value of the Slit Width is 2. nm, if you want to choose other Slit Width, press
【F1 】first, then press arrow key to choose the one you want and press 【ENTER 】to
confirm.
Blank
There is a system baseline stored in the memory of the instrument. Usually user may not
rebuild system baseline before test. Only putting the sample into the sample light path and
the reference into the Reference Light Path, the result can be obtained. As the system baseline
always get a little change after the instrument is powered on, it is necessary for the user to
rebuild the system baseline. There are a couple of ways to rebuild the system baseline. Select
“Yes” in Fig 5 or Press【】In Fig 73 or Press【F4】In Fig41,
Regarding Blanking, important points list below:
A. Take measure in Basic Mode
a. Put the reference cuvette with reference solution into the Reference Light Path and the
sample cuvette with reference solution into the Sample Light Path. Press the key 【ZERO】for
blanking.
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
Note 1. If the reference solution is too thick, “Energy Low…”will appear following the
“Blanking…”on the screen (Fig 8).If “Energy too Low…” appears following the “Blanking…”,the
test will be paused and “Warning…”will appear on the screen.(Fig 9)
2. If no automatic changer installed “cell #1” and “Max E” will disappear in Fig 8
Fig 8
3. DO NOT OPEN SAMPLE COMPARTMENT LID DURING BLANKING.
4. The dark current don’t be taken after power on if you bypass the calibrating system.
It is recommended to take the dark current after warm up.(See page 38).
Fig 9
b. Take out the sample cuvette, replace the reference solution with sample solution after
flushing the cuvette completely.Put the sample cuvette into the Sample Light Path, then the
result will display on the screen automatically. However the【START】must be pressed in
other measurements such as DNA/Protein, Muli WL and Quantitative etc.
B. Take measure in WL Scan
a. After all scan parameters are entered, put the reference cuvette with reference solution into
the Reference Light Path and the sample cuvette with sample solution into the sample light
path,Press 【START】to scan.
b. (Recommended) After all scan parameters are entered, put the reference cuvette with
Double Beam UV Visible Spectrophotometer BSDBU-201-B
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reference solution into the Reference Light Path and the sample cuvette with reference
solution into the Sample Light Path, Press 【ZERO】to obtain the user baseline. Then take out
the sample cuvette, replace the reference solution with sample solution after flushing the
cuvette completely. Put the sample cuvette into the Sample Light Path. Press 【START】to
scan.
Set wavelength (Example: set wavelength in “Basic mode”)
◦ Press【SETλ】(Fig 1 ).
Fig 1
◦ Use numeric keypad to input wavelength (Fig 11).
Fig 11
◦ Press 【ENTER 】to change the wavelength from 656.1nm to 45 . nm,and then blank;
After blanking, the screen displays as Fig 12.
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
Fig 12
Load or delete data or curve (Take the “WL scan” test For example)※
Press 【3 】in Fig.7 go into “WL scan”. After【LOAD】being pressed, the first file (ABC.wav)in
memory will appear on the bottom line of screen .Showed as Fig 13. Press 【 】∧or 【 】∨to
browse the files stroed in memory. Then if :
1. The key【ENTER】be pressed, the file selected will be loaded and displays on the
screen. Fig 14.
Note (1) The file selected must match “WL scan” test’s type. If not，the “file type error…” will
appear on the Right of top line.
(2) Different test has different file type. Refer to table 1 on Page 12.
2. The key 【CE】be pressed, the file selected will be deleted by selecting ”Yes”.
Fig 13
Fig 14
Double Beam UV Visible Spectrophotometer BSDBU-201-B
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Table 1
Test File Type
Quantitative Curve ***.t
Quantitative Test Result ***.qua
WL Scan ***.wav
Kinetics ***.kin
DN /Protein ***.dna
Multi WL ***.mul
WL Validity ***. wlv
ccu. Validity ***.phv
Save data or curve (Example: Save curve in “WL scan”)
• Press the key 【SAVE 】in Fig14 to save curve.
• Name the curve by pressing the numeric keypad (Fig 15), press the key【ENTER 】to
confirm.
. Note (1). Pressing numeric key continually to scroll characters and pressing 【 】∧, 【 】∨to
alter capital letter to miniscule. Table 2 shows all characters built in.
(2) If the name already exists in memory, the warning “duplicated name, are you
sure ” ？will appear . “Yes”for overwrite and“No”for Exit.
(3) The length of filename is less than 4
D2
W
Cell #1
WL : 680.0nm %T: 12 : 3 5: 2 7
2 0 0 .0 Wa velen gth (n m ) 6 8 0 .0
F om :
200.0
To:
680.0
S te p :
1.0n m
XS ca le
YS ca le
Plea s e in pu t File Na m e:2
0 %T 120 .0
Fig 15
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
Table 2
key representing key representing key representing
,+,-,* ,/ 1 1,#,?,:,I 2 2,A,B,C,=
3 3,D,E,F,% 4 4,G,H,I,{ 5 5,J,K,L,}
6 6,M,N,O,~ 7 7,P,Q,R,S, 8 8,T,U,V,“
9 9,W,X,Y,Z +/-/. -,.,
Print test report (For example: Print the report in “Basic mode”,Fig16)
Press the key【PRINT】to print the report (curve or data you have loaded or tested, Fig 17).
Fig 16
Fig 17
Before measurement
• Make a blank reference solution by filling a clean cuvette (or test tube) half full with
distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue to
remove the fingerprints and droplets of liquid.
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13 Analyze Sample
For different user’s requirements, we provide different test methods.
Basic Mode
Push the blank cuvette into the Reference Light Path and Main Light Path. In main menu
(Fig7),press【1 】to enter “Basic mode” test . After automatically blanking, it will display as Fig
18 (automatic changer installed) or Fig 19 ( automatic changer uninstalled) and wait for the
operator. 【ESC/STOP】to exit.
Note: If no automatic changer installed “cell #1” and “Max E” will disappear in Fig18
Fig 18
Fig 19
• Test
There are three modes (T%, Abs, conc/factor) for you to select by pressing 【F2】to make
choice.
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
Fig 2
1. Abs mode
Push the blank cuvette into the Reference Light Path and Main Light Path. Press 【F2 】to
select Abs mode ,Press 【ZERO】for Blanking , and then Push the sample into Main Light Path
to take reading(Fig 2 )
2. T% mode
The operation is the same as Abs test mode but pressing【F2 】to select T% mode .
3. Conc/Factor mode
Press 【F1 】to select a concentration unit (Fig 21). If no unit is suitable for your test, please
select the item “Other”, press【ENTER 】and input a new unit by pressing the numeric keypad
(Fig 22).
Fig 21
Fig 22
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4. Push the blank cuvette into the Reference Light Path and Main Light Path and press
【ZERO】for Blanking. There are now two choices for you to take:
4.1 Press【F3】to input known F value, Fig 23. Then push the sample into Main Light
Path to take reading of concentration
4.2 Push sample of known concentration into the Main Light Path
Press【F4】to input known Conc value, Fig 24. Then push the sample into Main Light Path
to take reading of concentration.
Note: 1.You can select wavelength at any time by pressing【SETλ. After your selection,
instrument always blanks automatically.
2.If F value is more than 9999,the clue of “out of range” will display on screen.
Fig 23
Fig 24
Print Test Report
Press 【PRINT】to print test results (Fig 25).
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Double Beam UV Visible Spectrophotometer BSDBU-201-B
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