Biotage TurboVap 96 User manual

Copyright 2010, Biotage AB. All rights reserved.
P/N 128097 Rev. 00
Concentration Workstation
TurboVap 96

User’s Manual

Preface 2
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Preface
Copyright
This manual is published by Biotage AB,10430 Harris Oaks Blvd. Suite C,
Charlotte, NC 28269 U.S.A. Copyright 2010, Biotage AB. All rights reserved.
Reproduction by any means or in any form of this manual or the products it
describes is prohibited.
Trademarks
Biotage and TurboVap are trademarks of Biotage. Microsoft and Windows are
either registered trademarks or trademarks of Microsoft Corporation in the United
States and/or other countries. All other trademarks and registered trademarks are
the property of their respective holders.
Content
The information in this manual may contain typographical errors or technical
inaccuracies and is subject to change without notice. Modifications may also be
made to the product described in this manual at any time.
Proper Equipment Operation
The TurboVap 96 Concentration Workstation is a microprocessor-controlled
instrument that provides simultaneous, automated concentration of multiple
samples, unattended operation, convenience, and speed. The TurboVap 96 can
simultaneously process one or two 96-well microplates or deepwell plates. It is the
customer's responsibility to determine suitable methods for evaporation of their
samples.
WARNINGS
• The TurboVap 96 is NOT designed for in vitro testing or for use with highly
corrosive acids.
• To reduce the risk of electric shock, do not remove the cover. No user
serviceable parts are inside. Refer to qualified service personnel if help is
required.
• Use this product only in the manner described in this manual. If the equipment
is used in a manner not specified by the manufacturer, the protection provided
by the equipment may be impaired.
AVERTISSEMENTS
• Pour réduire le risque de choc électrique, ne pas retirer le couvercle. Ce
produit ne contient aucune pièce pouvant être réparée par l’utilisateur. Au
besoin, confier l’appareil à un réparateur qualifié.
• Ce produit ne doit être utilisé que comme décrit dans ce manuel. Si cet
appareil est utilisé d’une manière autre que celle spécifiée par le fabricant, la
protection fournie par l’appareil peut être entravée.

Preface 3
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Product Safety Warning
The TurboVap 96 power switch is the same type of power switch used on most
laboratory equipment such as gas chromatographs, spectrophotometers, liquid
chromatographs, and computers. The TurboVap products are safely used in the
laboratory when “Good Laboratory Practices” are followed as with any other lab
equipment. All fans are brushless motor fans and will not ignite vapors. The
TurboVap is not classified as “Explosion Proof” and its use is at the discretion and
risk of the operator or laboratory supervisor/manager. Your TurboVap product has
been designed with safety as a foremost consideration. These products are
equipped with a non-arcing fan, solid state electronics, and provisions for vapor
collection. Do not change the configuration of the TurboVap 96 ventilation system.
Please consult with Biotage if you have any questions or concerns regarding this
subject.
WARNING
TurboVap products are NOT classified as “Explosion Proof.” The power switch is
an arcing source and could ignite explosive vapors if present.
Contact Biotage 1-Point Support
Biotage 1-Point Support provides expert services including telephone
troubleshooting of products, repair instructions, service dispatching and
replacement part information.
Before you call:
Check the online Help and the manual for a probable cause and solution to your
question.
Have the following information available for the customer support representative:
• Workstation serial number(s)
• If applicable, the error displayed on the LCD display
To reach the Biotage 1-Point Support:
US: 1-800-446-4752 or 1-[email protected]
Europe and Rest of the World: [phone] or [email protected]
Japan: [phone] or [email protected]
Biotage Product Repair Depot
The Biotage Product Repair Depot offers product repair services, upgrades,
refurbishment and installation at reasonable costs and with quick turnaround for all
customer-owned equipment and accessories. For further information or to obtain a
quotation for services, contact the Depot:
US: 1-800-446-4752 or 1-[email protected]
Europe and Rest of the World: [phone] or [email protected]
Japan: [phone] or [email protected]
Features of the Biotage Product Repair Depot:
• Factory-trained repair technicians
• Two week turnaround from receipt at Biotage

Preface 4
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Shipping:
Customers are responsible for shipments both to and from Biotage, specifying the
carrier and choice of service.
Return Policy:
To ensure a safe environment for all our technicians, it is mandatory for each
returned product to include our chemical questionnaire stating contact chemicals,
chemicals used during application and cleaning steps taken prior to shipment.
Processing:
Once the product is returned, it is evaluated for necessary repairs. The customer is
contacted with an estimate and may choose to go ahead with the repair or decline
service. If service is denied, a minimum evaluation charge may apply. Upon
completion of the repair, a purchase order or appropriate means of payment is
required before return shipment.
Service and Customer Support Plans
Biotage offers a full range of services to ensure your success. From our original
factory warranty through a comprehensive line of customer support plans, Biotage
AB offers you Field Service Engineers and In-house Specialists who are dedicated
to supporting your hardware, software and application development needs.
US: 1-800-446-4752 or 1-[email protected]
Europe and Rest of the World: [phone] or [email protected]
Japan: [phone] or [email protected]
Our programs can include such useful services as:
• preventative maintenance
• diagnostic servicing performed on-site by Biotage AB field service engineers
• extended use of the Biotage AB Customer and Technical Support Center
• automated, remote troubleshooting
• software updates
• after-hour, weekend and holiday support
• repair depot servicing
• parts, labor and travel expense coverage
• other customized services upon request
FCC
This device complies with part 15 of the FCC (United States Federal
Communications Commission) Rules. Operation is subject to the following two
conditions:
• This device may not cause harmful interference, and
• This device must accept any interference received, including interference that
may cause undesired operation.

Preface 5
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
CE
This device complies with all CE rules and requirements.
NOTE
Changes or modifications to this equipment not expressly approved by the party
responsible for compliance could void the user’s authority to operate the
equipment.
REMARQUE
Tout changement ou modification apporté à cet instrument non expressément
approuvé par l’entité responsable de la conformité peut annuler l’autorisation
d’opérer l’appareil accordée à l’utilisateur.
Table of Symbols
Table 1 contains symbols that identify particularly important information and alert
you to the presence of hazards. These symbols may appear in this manual and/or
on the product it describes.
Table 1. Important Symbols
Symbol
Symbole
Description
Description
DANGER: An imminently hazardous situation, which, if not
avoided, will result in death or serious injury.
DANGER : Situation présentant un danger imminent qui, s’il
n’est pas éliminé, peut entraîner des blessures graves, voire la
mort.
WARNING: Caution, risk of danger. Refer to the User’s
documentation.
AVERTISSEMENT : Attention, danger potentiel. Se reporter à la
documentation de l’utilisateur.
NOTE: A cautionary statement; an operating tip or maintenance
suggestion; may result in instrument damage if not followed.
REMARQUE : Énoncé indiquant une précaution à prendre, un
conseil de fonctionnement ou une suggestion d’entretien; son
non-respect peut provoquer des dommages à l’instrument.
Hazardous voltage; risk of shock injury.
Tension dangereuse; risque de blessure par électrocution.
Crush hazard. Risk of body parts, hair, jewelry, or clothing
getting caught in a moving part.
Danger d’écrasement. Faire attention que les parties
corporelles, les cheveux, les bijoux ou les vêtements ne soient
pas pris dans une pièce mobile.

Preface 6
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Risk of puncture injury.
Risque de blessure par piqûre.
Risk of eye injury; wear safety glasses.
Risque de lésion oculaire; porter des lunettes de sécurité.
Risk of exposure to biohazards.
Risque d’exposition à biohazards.
Risk of fire.
Risque d’incendie.
Risk of poison.
Risque d’empoisonnement.
Risk of explosion.
Risque d’explosion.
Hazardous fumes.
Émanations dangereuses.
Hot surface; risk of burns.
Surface chaude; risque de brûlures.
Protective ground symbol.
Symbole de terre de protection.
Ground symbol.
Symbole de terre.
Fuse.
Fusible.
Alternating current.
Courant alternatif.
On (supply).
Marche (alimentation).
Off (supply).
Arrêt (alimentation).
CE compliance mark.
Marque de conformité CE.
Signifies that the unit has passed safety tests for grounding,
power line transience, and current leakage.
Signifie que l’appareil a réussi les tests de sécurité pour la mise
à la terre, le courant transitoire de ligne d’alimentation et la
perte de courant.
Table 1. Important Symbols (Continued)
Symbol
Symbole
Description
Description

Preface 7
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Input.
Entrée.
Output.
Sortie.
Equipment
labels are color
coded:
Les étiquettes
de l’appareil
sont codées
couleur:
Yellow Caution, risk of danger
Red Stop
Blue Mandatory action
Green Safe condition or information
Jaune Attention, danger potentiel
Rouge Arrêter
Bleu Intervention obligatoire
Vert Condition sûre ou informations de sécurité
Table 1. Important Symbols (Continued)
Symbol
Symbole
Description
Description

Table of Contents 8
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Table of Contents
Preface .................................................................................................................... 2
Introduction .......................................................................................................... 10
Gas Vortex Shearing Action .............................................................................. 10
Microplate Heating ........................................................................................... 11
Vapor Removal ................................................................................................. 11
Automated Evaporation..................................................................................... 11
Adjustable ......................................................................................................... 11
Convenience ..................................................................................................... 11
Mild Conditions ................................................................................................. 11
Instant On Heat................................................................................................. 11
Cover Sensor .................................................................................................... 11
Requires No Exhaust Hood ............................................................................... 12
Multiple Sample Processing .............................................................................. 12
Microplates and Deepwell Plates....................................................................... 12
Hardware ............................................................................................................... 13
TurboVap 96 - Front .......................................................................................... 13
TurboVap 96 - Cover Open ............................................................................... 14
TurboVap 96 - Rear........................................................................................... 15
TurboVap 96 - Left Side .................................................................................... 16
Microplate Adapter ............................................................................................ 16
Front Panel ....................................................................................................... 17
Installation ............................................................................................................ 19
Unpacking the TurboVap 96 .............................................................................. 19
Parts Supplied................................................................................................... 19
Items Required But Not Included....................................................................... 19
Site Preparation ................................................................................................ 20
Installing the TurboVap 96................................................................................. 22
Connect the Gas Supply .............................................................................. 22
Install the Venting Hose ............................................................................... 23
Connect the Power Cord.............................................................................. 24
Turn On the Power ...................................................................................... 24
Power-Up Diagnostics ................................................................................. 24
Determining Optimal Concentration Conditions................................................. 25
Considerations when Selecting Plate Temperature .......................................... 25
Considerations When Setting the Gas Flow Rate ............................................. 26
Operation .............................................................................................................. 27
Preparing the Unit ............................................................................................. 27
Setting the Gas Time (Evaporation Time) for Position 1 .................................... 27
Setting the Plate Temperature for Position 1 ..................................................... 28
Setting the Flow Rate for Position 1 .................................................................. 28
Setting the Gas Time (Evaporation Time) for Position 2 .................................... 29
Setting the Plate Temperature for Position 2 ..................................................... 29

Table of Contents 9
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Setting the Flow Rate for Position 2 .................................................................. 30
Starting the Concentration................................................................................. 31
Elapsed Time Display .................................................................................. 32
Cycle Readout ............................................................................................. 32
Pausing the Concentration ................................................................................ 33
Restarting the Concentration............................................................................. 33
Completing the Concentration ........................................................................... 33
Maintenance .......................................................................................................... 34
Daily Maintenance............................................................................................. 34
Routine Maintenance ....................................................................................... 34
Replacing the Fuses.......................................................................................... 35
Long Term Storage ........................................................................................... 36
Troubleshooting .................................................................................................. 37
Troubleshooting the Concentration.................................................................... 37
Troubleshooting the Front Panel Display ........................................................... 38
Workstation Not Operational ............................................................................. 39
Specifications ....................................................................................................... 40
Index ...................................................................................................................... 43

Introduction 10
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Introduction
The TurboVap 96 Concentration Workstation is a microprocessor-
controlled instrument that provides simultaneous, automated
concentration of multiple samples, unattended operation,
convenience, and speed. The TurboVap 96 can simultaneously
process one or two 96-well microplates or deepwell plates.
Figure 1. TurboVap 96
Gas Vortex Shearing Action
The TurboVap 96 uses a patented “gas vortex shearing action” to
maintain high evaporation rates under mild thermal conditions.
When you close the cover, 96 gas nozzles in the manifold align with
each well of the microplate or deepwell plate. A helical flow of air is
created by the stream of gas directed into each well. The helical
flow sets up a vortexing action that provides for sample
homogeneity and continuous rinsing of the well wall. The TurboVap
96 enables you to independently control the gas flow rate for
Position 1 and Position 2.
Figure 2. Gas Manifold Nozzle in Microplate Well
Gas Manifold
Nozzle
Microplate Well
Gas Vortex
Shearing Action

Introduction 11
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Microplate Heating
Heaters below the microplate locating plates direct hot air through
holes in the microplate locators, toward each well in the microplate.
The forced hot air and the circulating gas quickly evaporates the
solvent in each well.
Vapor Removal
The solvent vapors are removed by a blower at the front of the
TurboVap 96 that blows air across the top of the microplates and
through the exhaust port at the back of the workstation. From the
exhaust port, the venting hose must be routed to a suitable outside
ventilation system.
Automated Evaporation
The TurboVap 96 uses an integrated microprocessor to
automatically time the evaporation, control the temperature, monitor
gas flow rates, automatically turn off the gas and heaters, and
perform operational diagnostics
Adjustable
The TurboVap 96 enables you to concentrate for a set time,
concentrate until stopped manually, and optimize the concentration
rate by adjusting the gas pressure and temperature.
Convenience
You can start a run of samples and leave the instrument
unattended. When the concentration has finished, the gas flow
turns off automatically, the temperature drops to 37° C, and the
workstation sounds an alarm every 30 seconds until you remove the
samples or press the Start • Stop button.
Mild Conditions
The heaters provide sufficient warming without harming the sample.
Instant On Heat
When you turn the workstation on, the heaters heat up to the set
Plate Temperature or to 37C if the temperature is set above 37C.
Cover Sensor
A sensor in the cover turns off the gas to protect from gas vapors if
the cover is opened during concentration.

Introduction 12
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Requires No Exhaust Hood
The cover and exhaust system permit the unit to be placed on a
bench rather than taking up valuable hood space.
Multiple Sample Processing
Each sample position provides 96 gas manifold nozzles that extend
into the wells of the microplates or deepwell plates to supply gas for
concentration. Using both position 1 and 2 allows up to 192
samples to be processed simultaneously.
Microplates and Deepwell Plates
Either one or two 96-well microplates or deepwell plates can be
used. Deepwell plates are placed directly on the surface of the
locator plate. Microplates use the supplied adapter to position the
top of the microplate at the same level as the top of a deepwell
plate.

Hardware 13
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Hardware
TurboVap 96 - Front
Figure 3. TurboVap 96 Hardware, Front View
Front Panel
Controls the setup and operation of the Concentration Workstation.
See “Front Panel” on page 17 for details.
Cover
Holds the gas manifold that directs the gas into the wells of each
plate to create the gas vortex shearing action.
Gas Regulator Knobs
Set the flow rate for all 96 gas manifold nozzles. The left gas
regulator knob controls the flow rate to Position 1; the right knob, to
Position 2. Pull out knob, turn, then push knob in to make
adjustments.
Front
Panel
Position 2
Gas
Regulator
Position 1
Gas
Regulator
Cover

Hardware 14
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
TurboVap 96 - Cover Open
Figure 4. TurboVap 96 with Cover Open
Gas Manifolds
Each manifold consists of 96 individual gas nozzles, which fit inside
each microplate well. Each nozzle supplies gas to concentrate the
sample in one microplate well.
Microplates and deepwell plates
Hold the samples for concentration.
Microplate Adapter
Used with microplates so that when the cover is closed, the
manifold nozzles fit inside each microplate well. Not used with
deepwell plates.
Heater assembly
Warms the samples during concentration to aid in evaporation.
Plate
Microplate
Adapter
Heater
Locations
Gas
Manifolds

Hardware 15
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
TurboVap 96 - Rear
Figure 5. TurboVap 96 Hardware, Rear View
Alarm Volume Control
Adjusts the loudness of the beeper.
Service Panel
Used by service personnel to access the exhaust blower and the
sensor/interconnect module.
Quick-disconnect Gas Inlet
Connects the gas supply to the workstation. The fitting has an
internal shutoff that allows you to disconnect the workstation from
the gas supply without shutting off the gas supply itself.
Exhaust port
Allows you to connect a hose to channel the solvent vapors to a
suitable outside vent location.
Power entry port
Connects the power cord and houses the fuse(s).
Alarm
Volume
Control
Service
Panel
Exhaust
Port Power
Entry
Port
Quick-disconnect
Gas Inlet

Hardware 16
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
TurboVap 96 - Left Side
Figure 6. TurboVap 96 Hardware, Left Side View
Power Switch
Turns the power on and off.
Microplate Adapter
Figure 7. Microplate Adapter
Microplate Adapter
Used to raise microplates up to the proper height. Not used with
deepwell plates.
Power
Switch
Position 1
Gas
Regulator

Hardware 17
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Front Panel
Figure 8. TurboVap 96 Front Panel
Display Indicator Lights
Indicate which setting is displayed in the Value and Units displays.
Gas Time - displays the length of time to evaporate, from 1 to 99
minutes. 00 evaporates until manually stopped.
Plate Temperature - the temperature, in degrees Celsius, of the
airflow under the plate. Range is from 20(ambient) to 80C.
Flow Rate - displays the total gas flow for all 96 nozzles in each
plate position. Range is from 5 to 99 standard cubic feet per hour
(SCFH). Adjust the flow rate using the gas regulators on each side
of the TurboVap 96.
Elapsed Time - the time, in minutes, that has elapsed since the
start of evaporation; “--” means not started. Range is from 0 to 99
minutes. If the Gas Time is set to manual mode (00), after 99
minutes the elapsed time displays “”.

Hardware 18
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Cycle Readout - when lit, all of the parameters, one at a time, for
any position that is running are displayed. If neither position is
running, the display shows the set values for both positions. The
plate temperature display shows the present temperature when
evaporation is in process for that position; otherwise, it shows the
set value.
Select Button
Advances the display indicator to the next option. If you press the
Select button after turning on the workstation, the TurboVap enters
diagnostics mode, displaying the current firmware revision.
Set Button
This button sets currently selected setting (Gas Time or Plate
Temperature) to the displayed value. The Set button only sets the
Gas Time and Plate Temperature. The Set button has no effect on
the other settings.
Up and Down Arrow Buttons
Increase or decrease the value in the display. Used to set the
values for Gas Time and Plate Temperature.
Start Stop Buttons
Start or stop evaporation. During evaporation, the Position light is
green.
Over Temp Light
Turns on (red) when the present temperature of one of the position
is 10C greater than the set plate temperature. When the
temperature drops below this value, the light turns off. The light may
indicate a heater runaway caused by a defective relay or
temperature sensor.
Low Flow Light
Indicates a low gas flow rate, possibly because the gas is running
out, or the flow regulator was not adjusted before starting the
evaporation. If the flow rate drops below 5 SCFH when the
evaporation is in process, the Low Flow LED lights and the alarm
beeps continuously.

Installation 19
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Installation
This section describes how to unpack the TurboVap 96, how to
prepare the site, and how to install the TurboVap 96.
Unpacking the TurboVap 96
Unpack the shipping container and verify that all parts listed below
are included. If any part is damaged or missing, contact Biotage
(see “Contact Biotage 1-Point Support” on page 3).
Parts Supplied
The TurboVap 96 includes the following parts:
• Concentration Workstation
• Power Cord(s) - 220V models include two power cords, one for
EU and one for UK
• Venting Hose 12.5 ft (3.8 m)
• 3/8 in. (9.5 mm) ID Gas tubing
• Bag of two 3/8 in. (9.5 mm) Barb Fittings (1/8 in. NPT and 1/4 in.
NPT)
• Coupling Insert
• Two hose clamps
• User’s Manual CD
• Microplate Adapters (2)
Items Required But Not Included
• Inert gas supply (nitrogen) with two-stage regulator

Installation 20
P/N 128097 Rev. 00 TurboVap 96 User’s Manual Biotage AB
Site Preparation
Before installing the TurboVap 96 Concentration Workstation, you
must have an appropriate location with available gas and electrical
sources and adequate ventilation as specified by these site
preparation requirements.
Space
Minimum bench space needed for the Concentration workstation
and any accessories:
• Height: 12 inches (30.5 cm)
• Width: 23.5 inches (59.7 cm)
• Depth: 12.5 inches (31.8 cm)
• Open Cover: 21 inches (53.3 cm)
Work Area
Flat, level, stable surface
Indoor use only within 12.5 ft (3.8 m) of proper ventilation
Gas Supply
You must use clean, dry, regulated nitrogen or other suitable gas.
Use the supplied 3/8 in. (9.5 mm) ID gas tubing for connection of
the gas supply.
A carbon trap is also recommended.
Use of compressed air could contribute to the oxidation of
some phenolic compounds and reduce sample recoveries.
WARNING
Never use hydrogen or other flammable gases. The wrong gas may
explode or catch on fire.
Compressed Gas Supply Inlet Pressure
1 bar = 100 kilopascals.
Devices added to the inlet supply line, such as moisture traps or
filters, must not drop the pressure under 30 psi (2.1 bars) measured
at the TurboVap 96 inlet fitting.
Minimum Recommended Maximum
30 psi (2.1 bars) 60-70 psi (4.1 - 4.8 bars) 80 psi (5.52 bars)