BRINTER ONE User manual

BRINTER®ONE
Printing life one layer at a time!™
User Guide
July 29th, 2022

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Contents
1Safety Information.................................................................................................................................... 5
1.1 General Safety Information ............................................................................................................ 5
1.2 Unpacking and Assembly................................................................................................................ 7
1.3 Protective Equipment ...................................................................................................................... 7
1.4 Hazardous Materials........................................................................................................................ 7
2Specifications............................................................................................................................................ 8
2.1 Product Configuration of Brinter® One, versions 1.0–1.x........................................................... 8
2.2 Product Configuration of Brinter® One, version 2.0 ..................................................................10
2.3 Technical Specifications................................................................................................................12
3Getting Started........................................................................................................................................13
3.1 Setting up the Brinter®One Platform .........................................................................................13
3.2 Setting up the Brinter®Software .................................................................................................13
3.3 Updating the Brinter®Software....................................................................................................14
3.4 Bioprinting with Your Brinter®One.............................................................................................. 14
3.4.1 Resetting the Pressure Display............................................................................................14
3.4.2 Configuring the UV Operations Parameters.......................................................................16
3.4.3 Configuring the Print Head Settings ...................................................................................17
3.4.3.1 Configuring the Pneuma Tool and Visco Tools..........................................................20
3.4.3.2 Configuring the Granu Tool ..........................................................................................21
3.4.3.3 Configuring the Pneuma Tool Pro, Pneuma Tool Cooled and Pneuma Triaxial ..22
3.4.3.4 Configuring the MicroDroplet Tools ............................................................................24
3.4.3.5 Completion of the Material Configuration.................................................................25
3.4.4 Slicing the STL Files...............................................................................................................27
3.4.4.1 Setting the Print Surface ..............................................................................................28
3.4.4.2 Importing the Objects ...................................................................................................31
3.4.4.3 Setting the Slicing Parameters for Objects ...............................................................33
3.4.4.4 Slicing the Objects.........................................................................................................35
3.4.5 Setting up the Printing Process ...........................................................................................36
3.4.6 Testing the Material Flow .....................................................................................................37
3.4.7 Calibration............................................................................................................................... 38
3.4.7.1 Manual Calibration of a Single Print Head ................................................................38
3.4.7.2 Manual Calibration of Multiple Print Heads ..............................................................40
3.4.7.3 Automatic Calibration of Multiple Print Heads .........................................................42
3.4.8 Controlling the Printing Process ..........................................................................................44
4Maintenance............................................................................................................................................48
4.1 Service Responsibilities ................................................................................................................48
4.2 Special Tools ...................................................................................................................................48

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4.3 Recommended Cleaning Agents .................................................................................................48
4.4 Preventive Maintenance Schedule..............................................................................................49
4.5 Preventive Maintenance Procedures ..........................................................................................49
4.5.1 Cleaning Procedures .............................................................................................................49
4.5.2 Inspection Procedures...........................................................................................................49
4.5.3 Lubrication Procedures.........................................................................................................50
4.5.4 Air Preparation Unit Preventive Maintenance Procedures..............................................50
5Spare Parts..............................................................................................................................................51
5.1 Ordering Information .....................................................................................................................51
5.2 Spare Part Classes.........................................................................................................................51
6Appendix 1. Print Bed Installation .......................................................................................................52
6.1 Heated Print Bed Overview...........................................................................................................52
6.2 Getting the Heated Print Bed Ready ...........................................................................................53
6.3 Using the Heated Print Bed ..........................................................................................................53
6.4 Temperature-Controlled Print Bed Overview.............................................................................. 54
6.5 Getting the Temperature-controlled Print Bed Ready ..............................................................54
6.6 Using the Temperature-Controlled Print Bed.............................................................................56
7Appendix 2. Visco Tool Manual.............................................................................................................57
7.1 Print Head Overview.......................................................................................................................57
7.2 Getting the Print Head Ready for Printing..................................................................................59
7.2.1 Inserting the Stator................................................................................................................59
7.2.2 Connecting the Dosing Unit to the Drive Unit ....................................................................60
7.2.3 Supplying and Bleeding the Material..................................................................................60
7.3 Cleaning the Print Head ................................................................................................................62
7.3.1 Quick Cleaning Protocol for the Print Head.......................................................................62
7.3.2 Disassembly of the Print Head ............................................................................................63
7.3.3 Cleaning of the Stator ...........................................................................................................65
8Appendix 3. Pneuma Tool Manual....................................................................................................... 66
8.1 Print Head Overview.......................................................................................................................66
8.2 Getting the Print Head Ready for Printing..................................................................................68
9Appendix 4. Granu Tool Manual ...........................................................................................................69
9.1 Print Head Overview.......................................................................................................................69
9.2 Getting the Print Head Ready for Printing..................................................................................70
9.3 Cleaning the Print Head ................................................................................................................71
9.3.1 Unclogging a Clogged Nozzle ...............................................................................................71
9.3.2 Cleaning the Granu Tool with ASACLEAN™ Purging Compound ....................................71
9.3.3 Cleaning the Granu Tool Mechanically ...............................................................................72
10 Appendix 5. Pneuma Tool Cooled Manual..........................................................................................75
10.1 Print Head Overview ..................................................................................................................75

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10.2 Getting the Print Head Ready for Printing..............................................................................75
11 Appendix 6. Pneuma Triaxial Pro Tool Manual..................................................................................77
11.1 Print Head Overview ..................................................................................................................77
11.2Getting the Print Head Ready for Printing..............................................................................78
12 Appendix 7. MicroDroplet Tool Manual............................................................................................... 80
12.1 Print Head Overview ..................................................................................................................80
12.2 Getting the Print Head Ready for Printing..............................................................................80
13 Appendix 8. Visco Tool Bio Manual......................................................................................................82
13.1 Print Head Overview ..................................................................................................................82
13.2 Getting the Print Head Ready for Printing..............................................................................84
13.2.1 Filling the Bioprinting Cap ....................................................................................................84
13.2.2 Installing the Bioprinting Cap...............................................................................................85
13.2.3 Dismantling of the Bioprinting Cap.....................................................................................86

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1Safety Information
1.1 General Safety Information
Please review the entire Brinter®user manual, as well as the appended manuals of any relevant print
heads and modules, before you start using Brinter®bioprinter. Any misuse can lead to equipment
damage or serious injury.
Brinter®One is intended only for research use by trained professionals familiar with the 3D
(bio)printing processes and their related risks. Use Brinter®One for its intended purposes only.
Brinter®One and its print heads and modules are for indoor use only. Always keep the Brinter®One
and its accessories in a dry environment at room temperature, in a well-ventilated place, and at a
minimum distance of 50 cm from other objects.
Do not try to modify its print heads, modules, or accessories. Do not open or disassemble Brinter®
One or attempt any service other than the instructions described in the Maintenance chapter.
Brinter®platform and other optional modules, such as print heads, include
moving parts that can cause injury. Never place your fingers inside the Brinter®
or near any moving parts while the printer is in operation. Tie back long hair or
clothing that can get caught in the moving parts. Never remove any parts from
the machine or from the optional modules when using.
Never clean or service Brinter®while the machine is on. Always turn off the power
by disconnecting the external power source and air pressure before cleaning or
servicing Brinter®One.
Brinter®uses UV light for disinfection and curing. Never look directly at UV light
or expose skin to UV light. Eye or skin exposure can result in serious personal
injury. This symbol indicates the presence of UV light in the print head.
Brinter®has heated surfaces. Never touch heated surfaces (e.g., the nozzle and
the barrel of the Granu Tool) when using the heating function. The heated
surfaces can reach very high temperatures of up to 300 °C, which are harmful
to the human body. Always allow time for the print heads and print bed to cool
before handling. It can take up to 30 minutes for the heated surfaces to cool
down completely. Never remove the barrel cover of the Granu Tool, i.e. the cover

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around the hot segment of the extruder. Never touch recently extruded plastic.
Hot plastic can stick to your skin and cause burns.
Never place or use flammable materials on or near the Brinter®when it is
powered on or in operation. Liquid acetone, alcohol, or other chemicals may
release extremely flammable vapors. When using the heated modules, take care
of the appropriate ventilation. Usage of a smoke detector connected to a fire
alarm is strongly recommended.
Plastic or other materials processed with the Brinter®can release volatile organic
compounds or toxic fumes above regulated threshold limit values. Refer to the
Safety Data Sheets of the materials being processed for more information and
use the Brinter® in a well-ventilated place. When printing with heated print heads,
the usage of a fume hood is strongly recommended.
Brinter®can radiate radio frequency energy. The operation of this equipment is
likely to cause harmful interference to radio communications, in which case the
user will be required to correct the interference at his own expense.
Always ensure that Brinter®is correctly mounted and placed before use, as
improperly mounted print beds, print heads, or modules can be dangerous. Never
tamper with any safety interlocks. Tampering risks equipment damage and
personal injury.
Never disassemble the Brinter®. All repairs must be performed by a qualified
technician. Any attempted repairs or modifications are not covered under the
warranty. Never open the switchboard, electric boxes or pneumatic box on your
own, or the warranty becomes void.
Never touch the electrical contact surfaces of the tool sockets in Brinter® One
version 2.0. There is a danger of potential electric shock and short circuits if the
contact surfaces are connected with a metal object, etc.

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1.2 Unpacking and Assembly
The Brinter®One is heavy; always use a minimum of two people to unbox and
move it. Read how to safely unpack and set up your Brinter®One from Chapter
3.1 Setting up the Brinter®One Platform.
1.3 Protective Equipment
Always wear protective gloves and eye protection when using the Brinter®,
including UV curing and disinfection process, etc.
Always wear protective gloves and eye protection while handling hazardous
materials, including materials that are toxic, corrosive or carcinogenic.
1.4 Hazardous Materials
Consult material safety data sheets, packaging labels and the manufacturer or
distributor catalog before handling hazardous dispensing materials, such as
flammable and corrosive materials. Always use appropriate protective
equipment and clothing.
After using ethanol to disinfect Brinter®, wait at least 60 seconds before starting
the printing process to ensure that all flammable vapors have been evaporated.

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2Specifications
2.1 Product Configuration of Brinter® One, versions 1.0–1.x
Brinter®One, versions 1.0–1.x, are equipped with four tool sockets attached to the protective door.
1. Print head / Tool socket
2. Print head
3. Print bed
4. Light gate for needle calibration
5. Positioners for the print surfaces (Petri dish/well plate)
6. Display for the pneumatic pressure
7. Protective door and hatch
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8. UV & Visible LED light module (Optional)
9. Tool changer interface
10. Tool adapter
11. Robotic arm
12. Pneumatic connector
13. Ethernet connector
14. External power supply connector
15. Device type plate with the serial number
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2.2 Product Configuration of Brinter® One, version 2.0
Brinter®One, version 2.0, is equipped with four tool sockets attached to the frame of the printer
instead of the door. These sockets are electrified and thus can keep the print heads at the set
temperature (heated or cooled). Furthermore, the design of the print heads for version 2.0 differ from
the print heads for version 1.x and hence cannot be used in the older Brinter®devices. Other features
of Brinter® One, version 2.0, are similar to version 1.x.
1. Tool socket rack for four print heads
2. Electrical contact surfaces of the tool sockets (NB: Never touch or put any metal objects on
the electrical contact surfaces as there is a risk of electric shock.)
The tool socket rack can be opened by pushing down the lever and turned aside to get easy
access to the print bed.
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3. Lever for opening the tool socket rack
4. Tool socket rack turning aside from its park position
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2.3 Technical Specifications
Power input: 100–240 VAC, 50/60 Hz, 1.3–2.5 A
Air pressure input: 6–8 bar
Weight: 44 kg
Frame height: 600 mm
Frame width: 550 mm
Frame depth: 650 mm
More detailed space requirements for the Brinter®One platform can be found in the onsite
preparation document.
Recommended operating temperature: 20–23 °C
NB: In normal operating conditions, the chamber temperature of the Brinter®One will rise 1–3
degrees above the ambient temperature.
Manufacturer
Brinter Ltd
Itäinen Pitkäkatu 4 C
20520 Turku
FINLAND

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3Getting Started
3.1 Setting up the Brinter®One Platform
The onsite preparation document gives the general process description on how to proceed at the
customer site to set up the Brinter®One platform onto the selected location.
Carefully unpack the Brinter®One from the packaging.
Lift the Brinter®One onto the selected location with the help of a Brinter®certified engineer or
customer’s engineer. The Brinter®One is heavy; always use a minimum of two people to move it.
Check (together with the Brinter®engineer) that the required power, air, and Ethernet connections
are available. Brinter®engineer installs Brinter®One if the installation has been ordered.
Brinter®engineer goes through the Customer delivery checklist and confirms that the equipment has
been received in intact condition. If the installation has not been ordered from the supplier, the
customer should immediately inspect the equipment in the case of any external damage to ensure
that the delivery is in accordance with the order. Any defects or deficiencies detected in conjunction
with the delivery shall immediately be reported in writing to the supplier.
In the case that the Brinter®engineer does not carry out the installation, start the installation by first
connecting the pneumatic hose to the pneumatic connector [12] by simply pushing the hose into the
fitting until it locks. To release, push the grey ring around the hose and pull out the hose.
Next, connect Brinter®One to the computer by plugging a network cable into the Ethernet connector
[13]. Connect the other end of the cable to a router or Ethernet port. Ensure that the router is switched
on before turning the Brinter®One platform on to ensure that the Brinter®fetches the correct IP
address.
Finally, turn Brinter®One on by plugging the external power supply into the connector [14].
3.2 Setting up the Brinter®Software
The Brinter®software is a web browser-based user interface that requires no downloads. We
recommend using the Google Chrome browser to ensure flawless operation. For optimal process,
use only one computer connected to Brinter®at a time.
Open the Google Chrome browser.
Check the serial number of your device; it can be found from the type plate located at the back of
your printer.
Connect to your Brinter®One platform by typing the serial number of your device into your browser’s
address bar: “http://brinterXXXX/”, in which you replace the “XXXX”with the serial number.
Brinter®One platform can also be connected by typing the correct IP address into the address bar
(for example, 192.168.100.44). Consult your department’s IT support to get a valid IP address.
Microsoft®Windows®users can also type “ping brinterXXXX”(where “XXXX”is the serial number of
your Brinter®) into the Command Prompt from the Start menu to find out the correct IP address.
MacOS®X and Linux®users are advised to contact Brinter®support ([e-mail]) for specific
instructions on finding the IP address.
Preferably use Incognito mode or private window mode of the browser.

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3.3 Updating the Brinter®Software
In order to update the Brinter®software remotely, Brinter®has to be connected to the Internet via
DHCP server assigned IP address. If no DHCP server is available, Brinter®will fall back to the following
static settings: IP 192.168.100.20, gateway 192.168.100.1.
Notice that the Ethernet cable must be connected (to a functioning router) before connecting the
power cable to Brinter®.
If the Ethernet connection is changed while powered on, Brinter®must be restarted to get the new
IP address.
If the computer connected to Brinter®One has the same rules for outgoing connections as Brinter®
One itself, you can test whether the connection to the update server is available. In Win 10
environment, this is done with PowerShell. Enter the following command in PowerShell: “Test-
NetConnection 194.100.97.73 -Port 2444”(case sensitive). If the answer has the row
“TcpTestSucceeded: True”, the connection can be established. Otherwise, check that outgoing port
2444 (TCP) is open.
Finally, outgoing port 2444 (TCP) must be open to IP 194.100.97.73 for the remote software update.
Ask your IT department to take care of this.
3.4 Bioprinting with Your Brinter®One
The pneumatic air must be turned on for the entire printing process. The incoming pressure should
be adjusted between 6–8 bar and should be kept at least at 5 bar for the tool changer to work
correctly. Also, if printing with the maximum pressure of 6 bar, set the incoming air pressure
significantly higher than the printing pressure, as some pressure is always lost in the transfer.
After the Brinter®One has been turned on by plugging in the external power supply, connect to your
Brinter®One platform, as explained in the text above. The plugging in starts an autocalibration
process in the Brinter®One. Wait for the calibration process to end before proceeding onwards.
Note that if you need to switch off the Brinter®One in the middle of the operation, wait for 2 minutes
before switching it back on again by plugging in the power supply. Brinter®is equipped with an
uninterruptible power source (UPS) that provides emergency power in the case of a short input power
source or mains power failure.
The actual bioprinting process is started from the Setup tab of the Brinter®software’s user interface.
There you can configure the parameters for the print heads and the optional UV module.
3.4.1 Resetting the Pressure Display
For the first time operation or, if otherwise needed, perform a zero-clear of the pressure display
without the pressure to adjust the displayed pressure to zero. The display is reset to zero in the
measurement mode when the up and down buttons are pressed simultaneously for 1 second or
longer. The main display shows [- - -], and the pressure is reset to zero. The display returns to
measurement mode automatically.

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1. LCD display –Displays the current status of pressure, setting mode, selected display units
and error code.
2. Unit display –Indicates the units currently selected.
3. button –Increase mode and ON/OFF set values.
4. button –Decrease mode and ON/OFF set values.
5. button –Press this button to change the mode and to confirm settings.
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3.4.2 Configuring the UV Operations Parameters
From the Setup tab of the Brinter®software, you can find a button for the optional UV operations.
From there, you can start the disinfection process (with 265 nm UV light), which should be used
before beginning the bioprinting. The UV light can kill or damage any living organisms inside the print
chamber, so remove or cover all cell-containing bioinks or prints before initializing the disinfection
cycle. From the same menu, a manual post-curing operation (365 nm or 405 nm) can be started.
1. UV operations –Click to configure settings for the UV disinfection cycle or manual post-curing
(LED module available for extra purchase).
2. Disinfection –Select the surface to be disinfected: Petri dish, entire print bed, or well plate.
3. Wavelength –Select the wavelength for the post-curing.
4. Intensity –Adjust the photocuring intensity (0–100%).
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5. Duration –Adjust the duration of the photocuring in seconds (0–600 s).
6. Run curing –Click to start the post-curing process.
7. See curing manual –Click to prompt a manual that helps to choose the correct curing
distance (the distance of the object from the light source).
8. Progress indicator –Shows that the disinfection process has started successfully.
3.4.3 Configuring the Print Head Settings
After the optional disinfection cycle:
Load the syringe barrel or several barrels with the bioink(s) by following the instructions given in the
Appendices for each specific print head. Brinter®One can use up to four print heads to print into the
same construct.
Mount the filled syringe barrel into the chosen print head by following the specific instructions given
in the Appendices.
Remember to connect the pressure connectors to the print heads securely.
Insert the print head(s) into the socket(s) on the Brinter®door. Brinter®software will then
automatically identify via RFID tags, which print head(s) have been mounted.
1. Print head selection –Click the image of the print head to start configuring the printing
parameters. Configure at least one print head before proceeding to the Slicer tab.
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2. Add new material –Create a new material profile.
3. Choose material –Click to choose an existing material profile or default material from the
database.
4. Edit material –Edit an existing material profile.
5. Delete material –Delete an existing material profile.
ADD NEW MATERIAL and EDIT MATERIAL buttons will prompt a new menu for the configuration of
the printing parameters.
6. Name –Give a name for the material profile.
7. Description –Give a short description of the material profile.
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8. UV Photocuring with 365 nm light –On/Off toggle switches for using 365 nm
photocrosslinking during or after printing. (Optional Fixed LED module available for extra
purchase.)
9. UV Photocuring with 405 nm light. On/Off toggle switches for using 405 nm
photocrosslinking during or after printing. (Optional Fixed LED module available for extra
purchase.)
A new menu for the photocuring parameters with the Fixed Led module will be prompted after
switching the toggle switch from DISABLED into the ENABLED position.
10. Layers –Menu for the photocuring options.
11. Photocuring options –Perform curing after the first layer, after every layer, or set custom
interval for curing.
12. Curing interval –Number of layers printed without curing. Available only if the Custom interval
setting is chosen.
13. Intensity percent –Adjust the intensity of the UV light (0–100%) for layer-by-layer photocuring
during printing.
14. Duration –Adjust the duration of the layer-by-layer photocuring in seconds during printing.
15. Intensity percent –Adjust the intensity of the UV light (0–100%) for post-curing.
16. Duration –Adjust the duration of the post-curing in seconds.
17. Curing manual –Click the link to open a separate document about the measured intensity
values (mW/cm2)for layer-by-layer curing with 365 nm and 405 nm LEDs.
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3.4.3.1 Configuring the Pneuma Tool and Visco Tools
18. Needle diameter –Set the inner diameter (I.D.) of the needle in millimeters.
19. Needle length –Set the length of the needle in millimeters.
20. Syringe type –Select the type of material supply: internal or external. (External supply is not
in use in this Brinter®version →Hence internal is preselected.)
21. Syringe volume –Select the volume of the syringe barrel in milliliters.
22. Tool pressure –Set the extrusion pressure in millibars (0–6000 mbar).
23. PreFlow time –Compensate the lag time between the onset of the extrusion pressure and
the actual flow of the material by pausing the print head movement after the air pressure
valve is opened for a designated time (0–1000 ms). This results in more continuous
filaments.
24. PostFlow time –Compensate the lag time between the switching off the extrusion pressure
and the interruption of the material flow by pausing the print head movement after the air
pressure valve is closed for a designated time (0–1000 ms).
25. Save material –After configuring all the printing parameters for the material, click SAVE
MATERIAL.
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