Bulteh 2000 EKOMILK Bond User manual

IndustrialArea, VEREA PLAST building,
StaraZagoraBULGARIA
Tel./Fax:[phone];
Tel. :[phone]
e-mail [e-mail]
R
ULTRASONIC MILK ANALYZER
OPERATING INSTRUCTIONS

2
TABLE OT CONTENTS
KEY FEATURE 3
CONTROLS 4
PARTS AND ACCESSORIES 5
ANALYZER INSTALLATION 6
MILK SAMPLES 6
MAIN MENU 6
HOW TO ANALYZE THE MILK 7
PRINTING DATA (IN CASE OF MICRO PRINTER CONNECTED TO THE ANALYZER) 8
CLEANING 9
DATA TRANSFER 12
CALIBRATION 13
CALIBRATION VALUES SAVING 14
CONDUCTIVITY CALIBRATION 15
PH CALIBRATION 16
TIME & DATE CALIBRATION 18
PH MEASUREMENT-ADDITIONAL INFORMATION 18
PH ELECTRODE -REPLACEMENT 19
TRATABLE ACIDITY-ADDITIONAL INFORMATION 20
MILK CONDUCTIVITY -ADDITIONAL INFORMATION: 21
MICRO PRINTER -ADDITIONAL INFORMATION: 23
ERROR’SLIST 24
SOME REASONS FOR EKOMILK ACCURACY AND REPEATABILITY DEVIATIONS 25
GUARANTEE 26

3
KEY FEATURE
EKOMILK BOND isrobust, reliable, automated multi-parametermilkanalyzerproviding rapidtest
results for:Fat, Protein, SolidsNot Fat, Lactose, Density ,Freezing point, AddedWater,pH,
Temperatureand Conductivity infresh milk(cow, sheep and/orbuffalo, goat).RS-232&USB interface,
Microprinterand automaticdata captureareintegrated. Basedupon ultra-sound technology, the
instrument does not requireany costlychemicals, causticorreagents fortesting. EKOMILK BOND is
an exampleof accuracy and efficiency of bench chemistrymethods. Test results arecoming just in45
seconds!
Keyfeature:
Fast Analysis -allowsa large numberof measurements to be done;
Simple andlightweight design;
+12V DC and 220V (or110V optional)AC Powersupply;
Cost effective:
Low powerconsumption;
Very small quantity of milk required;
No acid orotherchemicals are used;
One yearfull warranty;
Measuring accuracy adjustment can be doneby the user;
RS 232 Interface and Data collection System;
ESC POS Printerbuilt in.
Environmental Conditions:
Ambient airtemperature 15° -35°C
Milk temperature 5° -35°C
Relative humidity 30% -80%
Electrical Parameters:
AC PowerSupply voltage 220V ±5% 110V +10/-15%
PowerConsumption 30W max
Mechanical Parameters:
Dimensions (WxDxH) 380 x310 x285 mm
Weight 5.5 kg
Measuring Parameters:
Fatness from 0,5% to 12% with accuracy ±0,1%
Solids non fat (SNF) from 6%to 12% with accuracy ± 0,2%
Milk density from 1,0260 g/cm3to1,0330 g/cm3± 0,0005 g/cm3
Protein from 2%to 6% with accuracy ± 0,2%
Lactose from 0.5% to 7% with accuracy ± 0,2%
Freezing Point from 0to -
1.000 °C± 0,015°C
Added waterto milkfrom 0%to 60% with accuracy ± 5%
pH from 0,00 to 14 pH with accuracy ± 0,02
Conductivity from 2to 20 mS/cm ±1 % (18°C)
Temperature from 0to 50 °C with accuracy ± 0,1°C
Measuring cycle 90 seconds 45 seconds
Data Collection System:
Data Collection System is able to store up to 120 measurements.
Measuring modes:
COW SHEEP BUFFALO CREAM HOMOG.OTHER

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CONTROLS
Fig. 1.Appearance
1. Select the work mode
2. Skip andsearch forwards& backwards
3. Confirmthe choice
4. Tube (sucker)
5. Plastic support
6. pH electrode
7. Temperature sensor
8. Measuring mug
9. Micro printer
Fig. 2. Controls on the rear panel
1. RS 232 -PC interface outlet
2. ON/OFF Switch forcarvehicleuse 12 V
3. 12 V DC Powersupply outlet
4. Fuse
5. Powerswitch
6. AC powerled
7. 12 V DC printersupply
8. RS 232 -Printerinterface outlet
9. USB -PC interface outlet
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8
7
2
1
6
4
3
EKOMILK
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5
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9

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PARTS AND ACCESSORIES
Item Description Qty
1. EKOMILK BOND TOTAL 1
2. User’s guide 1
3. Connector12V 1
4. CD Milk analyzersoftware tools 1
5. User’s guide (CD Milk analyzersoftware tools) 1
6. RS232 Cable (NullModem, Link cable) 1
7. Measuring mug 6
8. Plunger 1
9. Buffersolution Conductivity 500 ml
5.02 (±0,5%)mS/cm(18±0,1°C).
1
10. Buffersolution powder
pH 7.00 ± 0,01/20°C, pH 4.00 ±0,01/20°C, pH 10.00 ±0,01/20°C*
1
11. Ekoday (alkaline cleaner– concentrate) 1
12. Ekoweek (acid cleaner – concentrate) 1
13. Ekopower(acid cleaner – concentrate)(on request) 1*
14. USB cable 1*
* Optional

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ANALYZER INSTALLATION
Place the Analyzeron a table orany otherflat surface.
Place vertically the Analyzeron a table orany otherflat surface.
Setting of AC PowerSupply Voltage
Connect the AC power lead first to the analyzerand then to the mains socket.
Switch on the AC power.
Setting of 12V DC PowerSupplyVoltage
Connect the DC supplylead to 12V input on the analyzer(black is“-”)and then to theautonomous DC
supply (forexample car battery).
Switch on the DC power.
Warm up
When the powerison, the EON Trading logo and themessage WARM UP appearson the display.
When the “warmup”stage isoverinabout5minutes,message EKOMILK isshown on the display.
The Analyzeris ready to use.
Warning:
1. The covers ofthe Analyzer should never be removed while the power leads are connected.
2. Under nocircumstance should you trytorepairtheAnalyzer’s power leadyourself.Incase
ofpower lead damage, contactyour dealer to makethe repairs.
Remark:Do nottake intoconsideration the firstsample because itis likelytobe witha
deviation outofspecification.
MILK SAMPLES
Milksamples temperatureshouldbe between 5and 35°C. Ifthemilktemperatureisabove 35°C the
message HOT SAMPLE appears on the display.
If you tryto test cool(refrigerated)milkwhich has some milkfat/cream separated you will probablyget
wrong resultespeciallyforthe milkfat contents. In thiscase you need to warmup the milkup to 40 -
42°C first, mixthe milkinorderto solve theseparated fat, coolitdown to 20-25°C andthen you can
test it with the EKOMILK Analyzer.
The milkacidity of the milksamplemust be less than 25°T forcow, buffaloand goat milkand less than
28°T forsheep milk.
Use the milk sample only once. When the measuring is carried out, throw the sample away.
MAIN MENU
Press the MODE button only once. Press the search buttons , ▼to find desired mode:
rCOW MILK -analysis of cow milk
rSEND & CLEAR -data transferring mode;
rREC CHOICE -turning on/off the data collection system;
rCLEANING -cleaning in the end of working day
rCALIBRATION -calibration
rSYSTEM -manufacturer’s mode only
rSHEEP/BUFFALO/ GOAT/HOMOG. MILK -analysis of sheep/buffalo/goatmilk

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HOW TO ANALYZE THE MILK
Step 1:
Fill the measuring mug with milksampleto be measured. Dipthe tube (sucker) into the milksample
and place the measuring mug on the Ekomilk working surface.
Step 2:
pH and temperature measurement:
Gentlyremove the protective plasticcap from the pH electrode beforeproceeding with measurement.
Use de-ionized ordistilled waterto rinse the electrodebeforeuse. Thiswill remove impurities that
have adhered on the electrode body. Rinsingwill also serve to activate the electrode, especiallyifit
has been dehydrated(inwhich case you may have to soak the electrodeinwaterfrom 10 minutes
onwards (See Section“pH Measurement -Additionalinformation”).Fill anothermeasuring mugwith
the same milksample. Dipthe electrode and temperaturesensor(fig.1 –pos. 6,7)intothesample. Be
surethatthe electrode iscompletelyimmersed intothe sample. Stirthe mug gentlytocreate a
homogeneous sample. Place it on the Ekomilkworking surface. Formoreinformation, see “pH
MEASUREMENT -ADDITIONAL INFORMATION”.
Remark: EKOMILK BOND uses3sensorsformilkparametersmeasuring –ultrasound, conductivity
and pH. Load on both measuring mugs the same milk and to get correct results atonce.
Step 3:
Press MODE and by means of the search buttons , ▼select the desired mode:
rCOW MILK -analysis of cow milk
rSHEEP/BUFFALO/ GOAT/HOMOG.MILK -analysis of sheep/buffalo/goat milk
When the propertype of milk is displayed, press OK to startthe measurement.
Supplier ID selection:
The message NUMBER:001 appearson the display whilemeasurement isstarted (itdoes no matter
which measuring mode is activated - COW MILK or SHEEP/BUFFALO/ GOAT MILK).
NUMBER defines the ID ofsupplier.In thiscase itisnecessarytowrite inadvance alist byID’sand
names of suppliers.
rbutton ▼changes the cursorposition;
rbutton changes the overcursorvalue;
rbutton MODE cancelsthe changes and returns COW MILK on the display;
rbutton OK confirms the ID NUMBER and QUANTITY:0000.0 appears.
QUANTITYselection:
rbutton ▼changes the cursorposition;
rbutton changes the overcursorvalue;
rbutton MODE cancelsthe changes and returns COW MILK on the display;
rbutton OK startmeasurement.
The message WORKING appears on the display whilemeasurement is goingon.
Remarks:
1. How todiscardthe currentmeasurement? Whilethemeasuring resultsareonthe display, press
button ▼and then press button not releasingbutton ▼and then release both of them. The
message RECORD DISCARDED appears on the display.
2. The maximum numberof records is120. Ifyou tryto write overthen 120 records, NOMEMORY
SPACE errormessage appears on the display. There are 2 possibilities:
a. To send the datatoacomputer and clear(empty)the analyzermemory (See procedure
described in "Data transferring").
b. Deactivating ofdata storage

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Press button MODE and by means of the search buttons , ▼select REC CHOICE. Press OK.
The message RECORDDATA?Ywill appearon the display. Change Yto Nby means of the search
buttons , ▼and press OK to startthe measurement. Thisway the RECORD DATA modewill
remain turned off until the Milk analyzeris switched off.
When the measurement is completed the display shows the results forfollowing milk parameters:
F:FAT % T: TEMPERATURE °C
S:SOLIDS NON FAT (SNF)% TA:TITRATABLE ACIDITY
D:MILK DENSITY Z:CONDUCTIVITY
P:PROTEIN % PH:PH
FP:FREEZING VALUE W:ADDEDWATER TO MILK%
*FREEZING VALUE = FREEZING POINT [°C] *(-100)
Press ▼button to see the result forLactose parameter
L:LACTOSE
Press again ▼buttonto go backto the previous screen.
Warning:
1. Do notremove the measuring mugs while the measurementis going on. This mightcause
resultdeviations outofthe spec limits.
2. Do notmove theAnalyzer while themeasurementis going on. This mightcause result
deviations outofthe spec limits.
3. Incase ofair bubbles presentsin the measuring camera the message EMPTY CAMERA
appears on the display. Throw outthe sample and repeatthe measurement.
4. Incase ofmotorproblem the message MOTORERROR appears onthe display. Switchoff
the device, waitfor1minuteand then switch iton again. Ifthe problem persists, please contact
your dealer.
Remark:
1. To get precise pH results, calibrate the pH measurement systemat least twice weekly.
2. Ifsomeone of pH ortemperaturesensorsaredisconnected orthe received results areout of spec
limits orthereisdamage inthemeasuringsystem,you will have messageERR instead of pHor
temperature results.
3. The pH resultisvalidwhen thereisR(Ready)behind the valueon the display. Otherwise make a
new measurement to get a correct result.
4. pH measurement isstronglyrelated to the temperature. Ifthe temperaturesensordoes not work,
the pH result willbe referred to the default temperature of 25°C. The accuracy would be out of range.
5. Formoreinformation, see “pH MEASUREMENT -ADDITIONAL INFORMATION”and
“CONDUCTIVITY MEASUREMENT -ADDITIONAL INFORMATION”).
PRINTING DATA (IN CASE OF MICRO PRINTER CONNECTED TO THE ANALYZER)
The printingisabout of the current results onthe screen. Everypress on the arrow button prints the
same results again.
Formore information –see section “Micro printer– additionalinformation”

9
CLEANING
This section is togive directions for dailyand periodical cleaning ofmilk analyzers Ekomilk in
order to assure their long life and proper functioning.
ULTRASONIC SENSOR CLEANING
1. DAILY CLEANING
Daily cleaning is required when:
-The intervalbetween two consecutive measurements is morethan half of one hour;
-The daily job is finished.
1.1. Cleaning outwhen the interval between twoconsecutivemeasurementsismore than half
an hour.
Steps:
1.1.1. Fill the measuring mug withclean andwarm, but not hot, and clean water(40°-60°C).Dipthe
tube (sucker) into the waterand place the measuring mug on the Ekomilk working surface.
1.1.2. Pressthe MODE button once. Press the searchbuttons , ▼to select CLEANING option.
Confirmwith OK.The display shows message CYCLES01 -numberofcleaning cycles tobe done.
One cleaning cyclepumps the waterinandout of the sensorfive times. Press the search buttons ,
▼to setthe desired numberof cleaning cycles. Thisnumbercan change from 1to99. We
recommend one ortwo cleaningcycles to be chosen.When thedesired numberisdisplayed, press
OK to startthe cleaning. Whenthe CLEANING stage isoverCLEANINGEND isshown on the
display.
1.1.3. Remove the mug and throw away the muddy water.Repeat thisprocedureseveraltimes till
clean watercomes outfromthe Analyzer.
1.1.4. Done.
1.2. Cleaning outatthe end ofa working day.
Thisprocedureprevents formation and collection of fatand “Milkstone”deposits into the sensor.Milk
stone consists ofmilksolids, calcium, magnesium, iron, sulfates,etc. Milkand watermineraldeposits
become hardened andlayered on the sensorand vinylpipes innersurfaces, which contact with milk
and disturbs the milkanalyzerwork. Cleaning willbe effective ifareagent which attacks the “milk
stone”isused. We recommend 2% solution of the alkaline cleaner EkoDay to be used as adaily
cleaning solution.
Preparation of 2% EkoDay working solution:
a.Use pipette to add 10 mL EkoDay to glassware with 490 mL distilled water.
b.Put into a labeled container.
Take care this solutiondoes not contact youreyes orskin!
Attention!Use only2%EkoDayworking solution as acleaning agent.TheEkoDayconcentrate
can damage your analyzer!
Steps:
1.2.1. First clean the analyzerwith pure wateras it is described inprocedure 1.1. (see above).
Attention!Always clean analyzerwith purewater before using the cleaning agent. Using 2%
solution ofthe alkaline cleaner EkoDaywithoutfirstcleaning analyzer for removing the fats
and proteins will resultinfixing the “milk stone” to the surface.

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1.2.2. Fill the measuring mug with2%solution of the alkaline cleaner EkoDay (25°-40°C),dipthe
tube (sucker) into thecleaning solution andplace the measuring mug on the Ekomilkworking surface.
Put the analyzerincleaning mode, set 20 cycles andpress the OK button.When the CLEANING
stage is overCLEANING END isshown on the display.
1.2.3. Fill the measuring mug with clean water,puttheanalyzerincleaningmode, set 5cycles and
press theOK button.When thisprocedureisdone, remove themug and throw away the water.Fill the
measuring mug with clean waterand repeat this procedure 3-4 times.
1.2.4. Done
2. PERIODICAL PROCEDURE
To ensureagood workof the MilkAnalyzer itisadvisoryto clean the deviceat least once aweek
strictly performing underwritten procedure.
This procedure uses 5% solution of acid cleaner EkoWeek as a periodicalcleaning solution.
Preparation of 5% EkoWeek working solution:
a. Use pipette to add 25 mL EkoWeek to glassware with 475 mL distilled water.
b. Put into a labeled container.
Take care this solution does not contact youreyes orskin!
Attention!Use only5%EkoWeek working solution asacleaning agent.The EkoWeek
concentrate can damage your analyzer!
2.1. Perform the daily cleaning first.
Attention! Make surethat the analyzerisproperlycleaned with purewaterbeforegoingon to the next
item oftheprocedure. Mixing both cleaningsolutions-alkaline EkoDay and acid EkoWeek willresult
in forming “milk stone”.
2.2. Fill the measuring mug with 5%solution of the acidcleaner EkoWeek (25°-40°C),dipthe tube
(sucker) intothe cleaning solutionand place the measuring mug onthe Ekomilkworking surface. Put
the analyzerincleaning mode, set 40 cycles and press the OK button. Whenthe CLEANING stage is
over, CLEANING ENDis shown on the display.
2.3. Open the top coverby pressing its left side – fig. 3 – step 1.
2.4. Remove the plug as it is shown on the fig. 3 – step 2.
2.5. Insertthe plungerinstead of the plug – step 3.
2.6. Fill themeasuringmug with clean andwarm, but not hot, water(40°-60°C),dipthe tube (sucker)
into the waterand place the measuring mug on the Ekomilk working surface.
Move up and down the plungerseveraltimes. Remove the mug and throw away the water. Fill the
mug with clean and warm waterand repeat this step 4-5 times – step 3.
Fig. 3.Hand-cleaning
2.7. Take the plungerout of the hole. Wait until all watercomes out. Inserttheplungeragain and press
it to the bottom.
2.8. Remove the measuring mug and movethe plungerout.Put theplug firmlyand close the top
cover.
2.9. Done.

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EKOMILK HEAVILY CONTAMINATED SENSORS
CLEANING PROCEDURE
This procedure is to be applied to any type of EKOMILK series milk analyzers in orderto clean heavily
contaminated ultrasonic sensors from milk stone deposits. Milk stone is naturally laid on the ultrasonic
sensors inside wall during the measurement cycles. In case the Analyzer’s cleaning procedure
described in the User’s Guide is not properly and regularly appliedmilk stone deposits gradually fill
ultrasonic sensorinside volume. This process leads to increased measurement results deviations and
sensordamage. This procedure willhelp you to completely clean and recover such heavily
contaminated sensors:
CLEANING STEPS
1. Fill the measuring mug with clean and warm, butnot hot water (40°-60°C).
2. Press the MODE button once. Press the search buttons , ▼to select CLEANING option. Confirm
with OK. Set 1 or2cleaning cycles using the search buttons , ▼. When the desired numberis
selected, press OK tostartthe cleaning. When the CLEANING stage is over CLEANING END is
shown on the display.
3. Remove the mug and throw away the muddy water. Repeat this procedure few times till clean water
comes out of the Analyzer.
4. Fill the measuring mug with 20% solution* of EkoPowercleaning liquid. Select CLEANING option
again and set 99 cycles. Press OK buttons and waits till all cyclesare done. This usually takes about
one hourand a half. Then replace the used cleaning solution with fresh and repeat same procedure 5
-8 times. Afterawhileyou willprobably see some debris in the cleaning solution. It is not necessary to
clean the sensorwith waterif you need to break this cleaning sequence during the nighttime. Just stop
the cleaning and continue on the next day.
5. .Open the top coverby pressing its left side –fig. 3–step 1.Remove the plug as itisshown on the
fig. 3–step2. Insertthe plungerinstead of the plug –step 3. Fill the measuring mug with clean and
warm, but not hot, water(40°-60°C),dipthe tube (sucker) into the waterand placethe measuring
mug on theEkomilkworking surface. Pull up and down the plungerseveraltimes inordertopush milk
stone debrisout oftheultrasonicsensor.Remove the mug and throw away the muddy water.Fill the
mug with clean and warm waterand repeat this step 4-5 times.
WARNING:Sometimes separated milk stone debris maybe so big theycan notpass trough the
pipes and block up water flow. Inthis case never applyan extreme pressure to thesyringe
piston in order to blowoutthe choke up since this mayresultin sensor damage. Slowlypush
and pull the syringe piston in order to remove the choke up.
6. When the milk stone is completely removed please, apply the procedure described above in steps 2
and 3 in orderto remove cleaningsolution remnants.
7. Done
* Preparation of 20% EkoPowerworking solution:
1. Use pipette to add 25 mlof EkoPowerto glassware with 100 mldistilled water.
2. Pourinto a labeled container.
PH ELECTRODE CLEANING
When the daily job is finished, follow the sequence below forcleaning and storage of pH electrode.
1. Lift up the analyzerfront cover.
2. Rotate the BNC connectorcounter-clockwise and unlocks it gently -fig.4 -step 1;
3. Move out the pH electrode and the holdertogetherfrom pH electrode gear-fig. 4 -step 2.
4. Wash the electrodewith de-ionized water.
5. Fill the protective cap with storage solution.

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Remark:
The storage solution ispH 4buffercontaining 225 grams of KClperliter.KClwouldbereplacedby
NaClortable salt. Tap wateris also acceptable as storage media.
Attention:
Avoid storage in de-ionized water.
6. Put the protective cap on the pH electrode.
7. Mount the pH electrode on the analyzerinthe reverseorder.
Fig. 4.pH electrode demounting
pH
electrode
pH
input pH
BNC
pHelectrode
holder
pHelectrode
gear
Step.1 Step.2
Formoreinformationabout pHElectrode Cleaningand Storage procedure (See section “pH
MEASUREMENT -ADDITIONAL INFORMATION”).
DATA TRANSFER
Transfer requirements:
rMilk analyzerEKOMILK BOND
rPC with Milk Data 2001 program Version 1.3;
rRS 232 NullModem Cable orUSB cable
Communication setup:
Step 1
Connectthe milk analyzer EKOMILK BOND to the PC.
Switch off the milk analyzerand the PC.
Step 2
StartMilk Data 2001 (version 1.4)program.
Step 3
Press aMilkAnalyzer's MODE button once.Scroll with thesearchbuttons , ▼to select SEND&
CLEAR mode. Press OK.Whilethe data transferring, the message PLEASE, WAIT... ison the
Analyzer's display, followed by the message TRANSFER OK? Y (or N).

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On the bottom ofthePC screen aprogress barprovides visualfeedback about the progress of
transmission procedure
ATTENTION!
IN ABOUT 1-2 MINUTES A MESSAGE APPEARS ON THE COMPUTER SCREEN:
1. Transfer is successful
When transferiscompleted successfully, new rows areadded to the table. The message "The data
received successfully!OK appears on the computerdisplay.
Ifyou wanttoerase the dataand emptyAnalyzer'smemory -select with searchbutton , ▼
TRANSFER OK?Y and confirmwith button OK.The message “READY” registersthat thedeletion is
completed. Now, you can store another120 milk data records.
Ifyouwantkeep datain the Analyzer memory -press button MODE.Thisway the data remains in
Analyzer's memory and it is possible to transferit againto anothercomputer.
2. Transmission error
In case of a transmission errora warning message appears on the display:
The data received contains 1 error(s).Acceptdata? (YES or NO).
YES puts the correct data to the program table.
NO denies all data.
To overthissituation you have to keep the date on the milkanalyzerand to repeat the transferagain.
Selection of Ntothe question TRANSFER OK? Y/N will keep the data onthe analyzerfrom Milk
analyzerand willtransferitto the PC again.
If you wantkeep data inthe Analyzermemory-press button MODE. In thisway the datajustremains
in Analyzer.
3. No datareceived
This may be forone offollowing reasons:
1. Analyzer's memoryis empty;
2. The RS232 cable is notcorrectlyfixed or itis damaged;
3. MILK DATA 2001 setup is notcorrect
So please check:
-selection FROM MEMORY option from Setting menu -the program version must be 1.3 and higher;
-selection of the right COM portconnected to the Milk analyzer.
Repeat the transferselecting Nto the question TRANSFER OK? Y/N. Thiswillkeep the data on Milk
analyzerand willtransferitto the PC again.
If you wantkeep data inthe Analyzermemory-press button MODE. In thisway the datajustremains
in Analyzer.
Attention!TRANSFER OK? Y willerase alldata.
CALIBRATION
The Analyzer should be calibrated ifthe measuring accuracyfor one ormore milkparameters
is outofthe specified limits.
Calibration values determination:
-Determineamilk sample FAT contentsbymeansofaclassical method(forexample you can
use Gerber method);
-Test the same milk using Milk analyzer;

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-Subtractthe Milkanalyzer FAT value from FAT value achieved withclassicalmethod.Ifthe
difference value isinthe spec limits thereisno need of fatcalibration. Otherwise add thisdifference
value as a fat calibration value using the procedure described in “Calibration values saving”;
-Determine a milksample SOLIDS NON FAT (SNF) bymeans ofa classical method;
-Test the same milk using Milk analyzer;
-Subtract the Milkanalyzer SOLIDS NON FAT (SNF) value from SOLIDSNON FAT (SNF) value
achievedwith classicalmethod. Ifthe difference value isinthe spec limits thereisno need of SOLIDS
NON FAT(SNF) calibration. Otherwise addthisdifference valueas a SOLIDS NONFAT (SNF)
calibration value usingthe procedure described in “Calibration values saving”;
-Determine the milk sample DENSITY bymeans ofa densitymeter;
-Test the same milk using Milk analyzeragain;
-Subtractthe Milkanalyzer DENSITY value from DENSITY value achieved with classicalmethod. If
the difference value isinthe speclimits thereisno need ofmilk DENSITY calibration. Otherwise add
thisdifference valueas amilk DENSITY calibration value using theproceduredescribedin
“Calibration values saving”;
Note:Ifyouwillcalibrateboth SOLIDS NON FAT (SNF) and DENSITY parameters, firstcalibrate
SOLIDS NON FAT (SNF). Test the same milk using EKOMILK Analyzer again. Calibratethe
DENSITY only ifitisnecessary.
-Determineamilk sample PROTEIN contentsbymeans ofaclassical method (for example you
can use Kjeldahl method);
-Test the same milk using EKOMILK Analyzer;
-Subtractthe Milkanalyzer PROTEIN value from PROTEIN value achievedwith classicalmethod. If
the difference value isinthe spec limits thereisno need of proteincalibration. Otherwise addthis
difference value as aproteincalibration value using theproceduredescribedin“Calibration values
saving”.
-Determineamilk sample FREEZING POINT BY means ofaclassical method (for example you
can use Cryoscope);
-Test the same milk using EKOMILK Analyzer;
-Subtract the MilkanalyzerFREEZING POINT valuefrom FREEZING POINT value achieved with
classicalmethod. If the difference valueisinthe spec limits thereisno need offreezing point
calibration.Otherwise add thisdifference value as afreezing point calibration value using the
procedure described in “Calibration values Saving”;
Example1:
Freezing point by classicalmethod -(-0.548)
Freezing value by EKOMILK -(53.0)
Freezing point by EKOMILK -(-0.53)
Freezing value correction value=(-0.548)-(-0.53)=(-0,018)
Example2:
Freezing point by classicalmethod -(-0.548)
Freezing value by EKOMILK -(56.0)
Freezing point by EKOMILK -(-0.56)
Freezing value correction value=(-0.548)-(-0.56)=(+0,012)
Attention:
1. Ifyou need to calibrate both SOLIDS NON FAT (SNF) and FREEZING POINT, firstcalibrate
SOLIDS NON FAT (SNF). Testthe same milk usingEKOMILK Analyzer again. Calibrate the
FREEZING POINT only ifitis necessary.
2. The freezing pointcorrection will change also the Added water measuring result.
CALIBRATION VALUES SAVING
-To save anew calibration value press MODE button first.
-Press the search buttons , ▼to selectcalibration mode:

15
rCALIBRATION
-Press OK.
-The display shows PASS 1 -first password numberprompt.
-Set with search buttons , ▼the first password number. Press OK to confirm it.
-Apromptforthe second passwordnumberappearson the display - PASS 2.Enterthe second
passwordnumberthen inthe same way as the firstone. Enterthe thirdpasswordnumberwhen a
PASS 3 prompt appears on the display.
Note:Incase ofincorrectpassword isentered, amessage WRONG PASSWORDappearson
the display. Then make a fresh start.
In case theright passwordnumbersareentered the display will show FAT COW MILK -Pressthe
search buttons , ▼to select the milk parameterto be calibrated:
rFAT COW MILK -cowmilk fatness calibration;
rSNF COW MILK -cowmilk Solidsnon fat (SNF)calibration;
rDEN COW MILK -cowmilk density calibration;
rPROT COW MILK -cow milk protein calibration;
rFAT SHEEP/BUFFALO/GOAT MILK -sheep/buffalo/goat milk fatness calibration;
rSNF SHEEP/BUFFALO/GOAT MILK -sheep/buffalo/goat milk SNF calibration;
rDEN SHEEP/BUFFALO/GOAT MILK-sheep/buffalo/goat milk density calibration;
rPROT SHEEP/BUFFALO/GOAT MILK -sheep/buffalo/goat milk protein calibration
rFP COW MILK -cow milk freezing point calibration;
rFP SHEEP/BUFFALO/GOAT MILK -sheep/buffalo/goat milk freezing point calibration
rCONDUCTIVITY -conductivity calibration (See section “Conductivity calibration”)
rPH -pH calibration (See section “pH calibration”)
rTIME & DATE -time and date calibration (See section "TIME AND DATE CALIBRATION").
Select desired calibration parameter. Confirm it with OK.
-The inscription VALUE appears on the display.
-Press the buttons , ▼to setthe calibration value required.
-The numbercan range from -2,54 to +2,54 (at intervalof 0,02)forfat,proteinand Solids non fat
(SNF),from-12,7 to+12,7 (atintervalof0,10)fordensity and from-0,250to+0,250 (at intervalof
0,002)forfreezing point calibration .Thisnumberdefines the calibration value. When desired
value is set on the display, press the OK button to save it.
-Then thedisplay shows TOTAL and anumberequalto the totalcalibration valueforthismilk
parameter.The totalcalibration value isan algebraicsum ofall calibration values added forthismilk
parameter.
-The calibration value saving is completed.
Note:The total calibration value can range from -2,54%to+2,54%(forfat,protein and Solids
non fat(SNF)) ,from -12,7°Ato+12,7°A for density)andfrom-0,250 to+0,250 for
freezing point.In case abigger number has been achieved during acalibration procedure a
CAL OUT OF RANGE error message appears on the display.
CONDUCTIVITY CALIBRATION
Conductivity sensorneeds to becalibratedon aregularbasis(once inamonth).Thisassures the
accuracy of measurement. Ekomilkconductivity calibration isperformed at one specificvalue -5.02
(±0,5%)mS/cm (18±0,1°C).Forconductivity calibration, use onlyproducer’sconductivity buffer
solution.
Steps:
1. Switch on the analyzerforone hourin advance.

16
2. Beforecalibrating, itisnecessaryto clean the Ekomilkusing theproceduredescribed in
“CLEANING OUT -Weeklyprocedure”.
3. Fill the mug with conductivity buffer.Put the mug onto pos.4,5–fig.1thetube (sucker) to be into
the solution. Rinse the measuring system by using the CLEANING menu. Throw out the buffer.
4. Fill themeasuringmug withconductivity buffer5.02 (±0,5%)mS/cm(18±0,1°C).Placethe
measuring mug onto pos. 4, 5 –fig. 1 the tube (sucker) to be into the solution.
5. Press the searchbuttons , ▼to select CALIBRATION mode. Enterthe passwordas itis
described in“CALIBRATION VALUESSAVING” and selectthe Conductivity to be calibrated.
Confirmit with OK. The inscription LOAD CAL LIQUID appearson the display.Press OK.A
message WORKING ... appearson the display whilethe calibration isgoing on. When the
measurement iscompleted the display shows CAL FINISHED.The conductivity calibration procedure
is complete. Throw away the used buffer.
You may repeat steps 4 and 5 again forbetterresult.
Remark: Conductivity buffersolution can be used only once.
6. Aftercalibrating, clean the Ekomilkwith onlywaterusing the proceduredescribed in “CLEANING
OUT -Dailyprocedure”.
Formoreinformation see section “CONDUCTIVITY MEASUREMENT -ADDITIONAL
INFORMATION”)
COND. CALERROR
MESSAGE
PROBLEM&
CAUSES
REMEDY
CAL ERROR 64 Pump damage Try again to calibrate the milk analyzerIf the problem still
exists, the Pump is out oforder.Contact yourdealer.
CAL ERROR 65 Wrong buffer
solution
Fill the measuring mug with conductivity buffer5.02 (±0,5%)
mS/cm (18±0,1°C)and repeat the calibration procedure.
CAL ERROR 66 Powersupply
trouble
Switch off the milk analyzerand switch it on after10-15 sec.
Try again to calibrate the milk analyzerIf the problem still
exists, the Pump is out oforder.Contact yourdealer.
PH CALIBRATION
Attention:To get precise pH results, calibrate the pH measurement systembeforeyou measurethe
pH of the sample oratleast twice a week.
Formore information see section “pH MEASUREMENT-ADDITIONAL INFORMATION”.
Calibrationisan important partofelectrode maintenance. Thisassures not onlythat the electrode is
behaving properlybut that the system isoperating correctly. Itisrecommended that the userperform
at least a2-Point calibration usingstandardbuffersthatadequatelycoverthe expected measurement
range priortomeasurement. 1-Point Calibration can also be used forquick measurements.Ekomilkis
capableof 3-point calibration toensureenhanced accuracy throughout the pH measurement range.
The 3pointcalibrationoffersflexibility of calibratingat3internationallyaccepted calibration points
namely, pH 7.00, 4.00and 10.00. pH measurement istemperaturesensitive. AutomaticTemperature
compensation isperformed by themilkanalyzer.To activate the ATC, simplyplug the temperature
probe to the temperature input.

17
Attention:
Itis besttoselectabufferas close as possible totheactual pH value ofthe sample tobe
measured.
Use standard calibration buffers thatthe temperature and the sample solution are the same.
Steps:
1. Press the search buttons , ▼toselect CALIBRATION mode. Enterthe passwordas itis
described in“CALIBRATION VALUES SAVING” AND select the pH to be calibrated. Confirmitwith
OK. The inscription LOAD PH 7 LIQUID appears on the display.
This message indicates that pH 7.00 is ready forcalibration.
2. Rinse the electrode well with de-ionized water.(Donot wipe the electrode as thismay cause a
build-up of electrostatic charge on the glass surface!).
3. Fill ameasuring mug with thebuffer7.00.Place itonpos.8anddiptheelectrode and temperature
probe into the buffer,ensuring that the electrode iscompletelyimmersed into the sample. Stirthe
electrode gently in the buffer.
Once you have selected the correctsolution, Press OK keytoconfirm. The message WAIT,
PLEASE.... appearson the display. Wait forthe measured pH value to stabilize. The nextmessage
LOAD PH 4LIQUID willbe displayed whenthe reading isready.The Ekomilkiscalibrated to buffer
7.00.
NOTE. Press button▼tointerrupt calibration. Message CAL FINISHED appears. Confirmit with OK.
The 1-pointcalibration procedure is now complete.
For2 or3 point calibration perform the 1,2, 3 steps following the Ekomilk prompts until completion.
Attention:pH buffer solutions can be used manytimes.
COND. CAL
ERROR
MESSAGE
PROBLEM&
CAUSES REMEDY
CAL ERROR 41 Pump damage Try again to calibrate the milk analyzerIf the problem still
exists, the Pump is out oforder.Contact yourdealerto make
the repairs.
CAL ERROR 43 Powersupply
trouble Switch off the milk analyzerand switch it on again in 10-15 sec.
Try again to calibrate the milk analyzerIf the problem still
exists, the Pump is out oforder.Contact yourdealerto make
the repairs.
CAL ERROR 44 -pH electrode or
temperature sensor
is disconnected
-pH Electrode
damaged
-Temperature
probe damaged
-pH measuring
system is damaged
Check the connections.
Activate orreplace theout of orderpH electrode with a new
one -see “pH measurement -Additionalinformation”
Replace the out of orderTemperature probe with a new one.
Try again to calibrate the milk analyzerIf the problem still
exists, the pH measuring system is damagedis out of order.
Contact yourdealerto make the repairs.
CAL ERROR 46 Wrong buffer
solution Fill the measuring mug with right bufferand repeat the calibration procedure.
CAL ERROR 47 pH measuring
system is damaged
Switch off the milk analyzerand switch it on again in 10-15
sec. If the problem stillexists, thepH measuring systemis out
of order.Contact yourdealerto make the repairs.

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TIME & DATE CALIBRATION
Press the search buttons , ▼toselect CALIBRATION mode. Enterthe passwordasitisdescribed
in "CALIBRATION VALUESSAVING" and select TIME &DATE to becalibrated.Press OK to
confirm.
The message TIME:00:00:00 appears on the display:
button ▼changes the cursorposition;
button changes the overcursorvalue;
button MODE cancelsthe changes and returns COW MILK on the display;
button OK confirms the TIME and shows DATE:00:00:00;
button ▼changes the cursorposition;
button changes the overcursorvalue;
button MODE cancelsthe changes and returns COW MILK on the display;
button OK confirms the DATE and shows TIME SET OK.
If TIME &DATE Chipisnot installed ordoes not workproperlyoraincorrect Time orDate value is
achievedduring acalibration procedureaerrormessage ERRORSETTINGTIME appearson the
display.
PH MEASUREMENT-ADDITIONAL INFORMATION
1. General
pH isaunitto measurewhichdescribes thedegree of acidity oralkalinity of asolution. It ismeasured
on scaleof 0to14.The termpH isderived from“p”,themathematicalsymbolofthenegative
logarithm, and “H”,the chemicalsymbolof Hydrogen. The formaldefinition of pH isthe negative
logarithm of the Hydrogen ion activity.
2. pH Electrode
ForpH measurement Ekomilkneeds acombination electrode, compatiblewith most pH electrodes
that have BNC connectorsand zeropotential(thepH wherethe millivoltoutput oftheelectrode equals
0)near7 pH.
2.1. Electrode part
The electrode isthemost important part ofthepH measurement.The electrode glass membrane is
fragileand must behandled with care. To protect the glass membrane and to maintainactivation, the
glass membrane is covered by a protective rubbercap containing a suitable storage solution.
2.2. Electrode care & Electrode maintenance
pH Electrodes aresusceptibletodirtand contaminationand need to be cleanregularlydepending on
the extent and condition of use.At no time shouldonetouch orrub the glass bulbas thiscausesthe
build-up of electrostatic charge.
2.3. Storage
The best results, always keep the pH bulb wet. An optimalstorage solutionforcombination electrode
ispH 4buffer(cleat not pink)with 225 grams of KClperliter.Tablesalt, NaCl,can be used ifKClis
not reallyavailable. OtherpH buffersortap waterarealso acceptablestorage media, but avoid
storage inde-ionized water.The protective rubbercap filled withthe buffersolution provides ideal
storage forlong periods.
3. Electrode Lifespan

19
pH electrodes have afinite lifespan due to theirinherent properties. How longapH electrode willlast
willdependon how it iscaredand thesolution itisused tomeasure.Even ifan electrode isnotused it
still ages. Electrode demise can usuallybe characterized by asluggish response, erraticreadings ora
reading which will not change. When thisoccursanelectrode can no longerbe calibrated.pH
electrodes arefragile and have alimited lifespan. The harsherthesystem, theshorterthelifespan. For
thisreasonitisalwaysagood idea to haveaback-upelectrodeon hand toavoidanysystem down
time.
4. Buffer Solutions
Buffersaresolutions that have constant pH values andthe abilityto resist changes inthat pH level.
They are used to calibrate pH measurement system.
5. Automatic Temperature Compensation (ATC)
AutomaticTemperaturecompensation iscontained withinthe milkanalyzer,because pHmeasurement
istemperaturesensitive. To activate the ATC, simplyplug inthetemperatureprobe into the phono
jack.
6. pH Electrode Calibration
pH Electrodes arelike batteries;they run down with time and use. As an electrode ages, its glass
changes resistance. Forthisreason, electrodes needto be calibrated on aregularbasis. Calibration in
pH buffersolution corrects forthis change.
Calibrationisan important partofelectrode maintenance. Thisassures not onlythat the electrode is
behaving properly but that the system is operating correctly.
UsuallypHmetersrequirecalibration at 3specificpH values. One calibrationisusuallyperformedat
pH 7, second and third are typically performed at pH 4 and pH 10.
Attention: It isbesttoselect abufferasclose as possibleto the actualpH value of the sampleto be
measured. Use standardcalibration buffersthatthetemperatureand the samplesolution are the
same.
7. Milk acidityand pH
The following table shows the relation between pH value and °T.
°T Variations pH
(average value)
°T Variations pH
(average value)
Rawmilk Pasteurize milk
16 6,74 -6,70 6,72 16 6,68 -6,64 6,66
17 6,69 -6,65 6,68 17 6,63 -6,58 6,61
18 6,64 -6,58 6,62 18 6,57 -6,52 6,55
19 6,57 -6,52 6,55 19 6,51 -6,46 6,49
20 6,51 -6,46 6,49 20 6,45 -6,40 6,43
21 6,45 -6,40 6,43 21 6,39 -6,35 6,37
22 6,39 -6,35 6,37 22 6,34 -6,30 6,32
23 6,34 -6,30 6,32 23 6,29 -6,24 6,26
24 6,29 -6,24 6,25 24 6,23 -6,19 6,21
PH ELECTRODE -REPLACEMENT
pH electrodes have afinite lifespan due to theirinherent properties. How longapH electrode willlast
willdependon how it iscaredand thesolution itisused tomeasure.Even ifan electrode isnotused it
still ages. Forthis reason it is always a goodidea to have a back-up electrode on hand.

20
How to replace the electrode?
1. Demount the electrode as it is described in section “Cleaning. pH electrode cleaning”.
pH holder
2. Remove the damaged pH electrode.
3. Put the new one and screw it.
4. Mount the pH electrode on the analyzerinthe reverseorder.
5. Calibrate the new pH electrode followingthe 3points procedure
described in section “pH calibration”.
TRATABLE ACIDITY-ADDITIONAL INFORMATION
Thisoption transformspH value totitratableacidity (inone of the following units°Th ,%La, °SH or°D)
using on ofthe 3 tables of correspondence (1 default and 2 user’s)stored inthe analyzer memory.
There is notstrong correlation betweenpH and titratable acidity.
pH isaunitto measurewhichdescribes thedegree of acidity oralkalinity of asolution. It ismeasured
on scale of 0 to 14. The formaldefinition of pH is the negative logarithm oftheHydrogen ion activity.
Acidity canalso be expressedas the titratable. The titratableacidity of asolution ismeasured by
reacting the acids present with abase such as sodium hydroxide(NaOH)to achosen end point, close
to neutrality, as indicated by an acid sensitive colourindicator.
But the practice shows that forsome type of
milkthereisagood relation between thesetwo
parameters.
The usercan choosethe measuring unit,the
tableof transformation and can edituser’s
tables.
These procedures are performed by PC program
named pH2TA.EXE.
Steps:
1. Copy the file pH2TA.EXE from CD to some
hard disk of yourPC.
2. Connectthe RS232cableto some free COM
portto the PC andto the RS 232 connectorof
milk analyzer.
3. Switch on the milkanalyzer.
4. Press amilkanalyzer’s MODE button only
once. Thedisplay willshowthe message
CREAM MILK.
5. Press the button and nextpress without
releasing .
6. Releaseboth buttons. The display will show
the message Bulteh 2000 Bootloader 1.01.
7. Use Windows Explorer to run pH2TA.EXE.
8. The program window willappearon the PC screen.
9. Click on the COM PORT boxand the list of all availableCOM ports appears. Choose the COM port
connected to themilkanalyzer.Ifthe connection isdone the Connection Status will be Connection
OK. Ekomilk Currentsettings area shows the current values foracidity unit and table.
10. The milk analyzeris ready for changing the titratable acidity properties.