Byonoy Absorbance 96 Automate User manual

Manufacturer:
Byonoy GmbH
Schützenstraße 21
22761 Hamburg, Germany
00 49 40 5379 866 00
[e-mail]
www.byonoy.com
Instructions for Use
Absorbance 96 Automate
Valid for REF Number DE MAA 001
Instructions for Use
Absorbance 96 Automate
Version 1.0 (28/07/2023)
©Copyright 2023 by Byonoy GmbH. All rights reserved.

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Dear customer,
We are delighted that you have chosen Absorbance 96 Automate. To take full advantage of
the instrument's performance and to enjoy your instrument for many years, please read
these instructions for use carefully before installation and commissioning. Operate the in-
strument in accordance with these instructions. The operating-safety and function of the
instrument can only be guaranteed if both the general safety regulations and accident pre-
vention regulations of the legislator as well as the safety instructions in this manual are ob-
served.
Ensure that the manual is always accessible and is read and understood by all per-
sons operating the instrument.
This user manual may only be used according to its intended purpose. It may not be repro-
duced, changed, or translated in another language without the prior written consent of
Byonoy GmbH.
This document is subject to technical changes and updates.

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TABLE OF CONTENTS
1 General information....................................................................................................................................1
2 Overview of Absorbance 96 Automate ......................................................................................3
2.1 Scope of application .................................................................................................... 3
2.2 Measurement method ................................................................................................. 3
2.3 Absorbance 96 Automate System ............................................................................ 4
2.4 Consumables .................................................................................................................. 5
3 Preparing the product for use.......................................................................................................... 6
3.1 Unpacking, storage, and transport ........................................................................... 6
3.2 Supplied materials ........................................................................................................ 6
3.3 Working environment .................................................................................................. 7
3.4 Installation ...................................................................................................................... 7
4 Operation ......................................................................................................................................................... 9
4.1 App installation procedure and updates .............................................................. 10
4.2 Computer connection ................................................................................................ 10
4.3 Workflow ....................................................................................................................... 10
5 Signals and troubleshooting.............................................................................................................12
5.1 Signal lights .................................................................................................................. 12
5.2 Troubleshooting ......................................................................................................... 12
6 Maintenance and cleaning.................................................................................................................14
6.1 Maintenance ................................................................................................................ 14
6.2 Cleaning ........................................................................................................................ 15
6.3 Technical support ....................................................................................................... 16
6.4 Repairs .......................................................................................................................... 16
7 Technical specifications.......................................................................................................................17
8 Guarantee .......................................................................................................................................................19
9 Declaration of Conformity................................................................................................................. 20

General information
1
1 General information
Important: Follow the instructions for use
Any commissioning or handling of the instrument requires precise knowledge and notice of
this manual. The device is intended for the described use only.
In this manual and on the label of the reader, particularly important remarks are labeled as
followed:
Symbol
Description
Caution: Caution indicates a potentially hazardous situation, which, if not
avoided, could result in minor or moderate injury.
Information: This is a piece of information indicating certain properties that
must be observed.
CE-mark
Disposal of used electrical and electronic equipment
Manufacturer
Serial number
Consult instruction for use (user manual)
Catalogue number
Liability for function and damage
Liability for the function of the device shall, in any case, pass to the owner or operator if the
device is improperly maintained, repaired, or modified by persons who do not belong to the
authorized service personnel, or if it is handled in a way that does not comply with its in-
tended use. The service and operation of the product must be in accordance with this man-
ual. Byonoy shall not be liable for damages resulting from non-observance of the information

General information
2
above. Warranty and liability conditions of the terms of sale and delivery of Byonoy are not
extended by the information above.
Disposal of used electrical and electronic equipment
The symbol on the product or its packaging indicates that this product is not to be treated
as normal household waste. It must be disposed of at a collection point for the recycling of
electrical and electronic equipment. By contributing to the correct disposal of this product,
you protect the environment and the health of your fellow human beings. Recycling helps to
reduce the consumption of raw materials. For further information on how to recycle this
product, please contact your local authority or municipal waste disposal centres.
Recycling of the product may also be carried out by the manufacturer. Please contact the
service department.
Working with biological and harmful material
The Absorbance 96 Automate is not to be used for the measurement of biohazardous sub-
stances.
Always observe the manufacturer's hazard information pertaining to the substances to be
measured.
The instrument does not produce any toxic or harmful gases or substances. During the
measurement, make sure that there are no toxic or harmful substances in the microtiter plate.

Overview of Absorbance 96 Automate
3
2 Overview of Absorbance 96 Automate
2.1 Scope of application
Absorbance 96 Automate is an optical laboratory instrument for measuring the absorbance
(optical density) of biological or non-biological samples in ANSI/SBS-standard 96-well mi-
crotiter plates in accordance with the specifications described in the user manual. Absorb-
ance 96 Automate is an on-deck microplate reader module designed for easy integration
into various liquid handling or robotic systems.
Absorbance 96 Automate is intended for research and other non-in-vitro-diagnostic anal-
yses only. It is to be operated by trained laboratory personnel and is intended for profes-
sional use.
2.2 Measurement method
Absorbance 96 Automate is designed to carry out sensitive absorbance measurements. It
measures the optical density (OD) of samples at defined wavelengths.
Absorption
Absorption refers to the amount of light absorbed by a medium. Absorption reduces trans-
mission. Transmission is the ratio of incident to transmitted light. Accordingly, the degree of
transmission is calculated as follows
T = (I/I0)
where I is transmitted light and I0is incident light.
Optical Density
Optical density is a measure of the attenuation of light radiation after it has passed through
a medium. Optical density is the logarithmic quantity that describes the reciprocal of the
transmittance T:
OD = log(I0/I)
where I is the transmitted light and I0is incident light.
Optical density is the absorbance of the sample plus other attenuating effects such as scat-
tered light due to turbidity. It is therefore necessary to avoid scattered light in order to meas-
ure absorbance correctly.
If no other attenuating effects are present, absorbance = optical density.

Overview of Absorbance 96 Automate
4
2.3 Absorbance 96 Automate System
Absorbance 96 Automate Reader
Absorbance 96 Automate is an absorbance-based measuring device, i. e. a measuring instru-
ment, with which the absorbance values of a 96-well microtiter plate can be read, recorded
and provided for further processing. Absorbance 96 Automate is a solid-state microplate
reader and has 96 detection units, allowing measurement without a scanning mechanism.
Absorbance 96 Automate is primarily designed to be integrated on-deck of various liquid
handling and other robotic systems.
Figure 1.
Visual representation of the product from the front, top. 1. Illumination unit 2. Signal
light. 3. Groove for the gripper. 4. Detection unit. 5. USB-C cable. 6. SBS adapter. 7. Parking
position. 8. Microplate.
In the illumination unit of the measuring instrument there are up to 6 LED light sources with
different wavelengths and up to 6 interference filters. The signal light positioned at the top
of the illumination unit displays the current status of the reader, including its connection sta-
tus, initialization progress, measurement status, and any errors that may have occurred.

Overview of Absorbance 96 Automate
5
In the detection unit of the reader, 96 photodiodes collect the light passing through the mi-
croplate. The lower and upper parts of the reader are connected via spring contacts.
The microtiter plate is loaded onto the reader using the robotic system's gripper.
On the bottom of Absorbance 96 Automate there is a USB-C port for connecting the instru-
ment to a computer via the included USB-C cable.
Power consumption
Absorbance 96 Automate is an analytical instrument with very low power consumption. It is
operated by a USB C cable and the total power consumption is generally less than 2.5 watts.
Absorbance 96 App
The reader is controlled via the Absorbance 96 App. The operation of the app is described
in-detail in a separate user manual.
2.4 Consumables
With the Absorbance 96 Automate, ANSI/SBS Standard 96-well microtiter plates can be
used. Only certain microplate types can be used with Absorbance 96 Automate (see chapter
7).
When using microtiter plates, always check the specifications of the microtiter plate manu-
facturer. Not all microtiter plates of a particular manufacturer are the same in design, mate-
rials, or configuration. The temperature stability within the microtiter plate can depend on
the type of microtiter plate used.

Preparing the product for use
6
3 Preparing the product for use
3.1 Unpacking, storage, and transport
Unpacking
The device is packed in a specially designed cardboard box. Keep the packaging material. If
the device must be returned for repair, the original packaging material must be used.
Storage and protection during the intervals of normal use
Protect the device from moisture and dust during prolonged storage.
Do not store the device next to heat sources and protect it from direct sunlight.
The temperature for storage should be within the recommended temperature range (see
chapter 7).
Transportation
Before transportation, unplug the instrument and ensure there is no microtiter plate inside
the device. Depending on the transport distance, use the original packaging material. Make
sure that the new location meets the requirements described in chapter 7.
To avoid spillage and contamination, make sure that there is no microtiter plate
inside the instrument before transport.
Always transport the product well-packaged to avoid damage in transit.
3.2 Supplied materials
When unpacking the device, please check that the following components are present:
• Absorbance 96 Automate
o Illumination unit
o Detection unit (with SBS adapter)
o Parking position (with SBS adapter)
• USB-C cable
• Hex key
• Microfiber cloth
• Information card with the download link
• Calibration certificate

Preparing the product for use
7
3.3 Working environment
The Absorbance 96 Automate is designed to be integrated on-deck into liquid handling and
robotic systems.
However, the following warnings must be observed. Ignoring them may lead to measurement
errors and a reduction in the expected lifetime or damage to the device:
Do not operate the device near heat sources or under direct sunlight.
The ambient temperature and the humidity should be within the recommended
range (see chapter 7).
3.4 Installation
Before commissioning Absorbance 96 Automate, you should carefully read and understand
the entire manual to familiarize yourself with the system.
Absorbance 96 Automate is controlled via the Absorbance 96 App, including the analysis of
measurement data. The functions of the app are detailed in a separate user manual.
Commissioning Absorbance 96 Automate on-deck of a liquid handler entails three integra-
tion steps:
1. Placement on-deck
2. Preparing the liquid handler
3. Integrating with the software
Each of these steps are explained below in further detail.
Placement on-deck
Prior to setting up the reader, connect the detection unit to the USB-C cable as depicted in
Figure 2. Remove the SBS adapter by loosening the pair of screws. Insert the USB-C cable
and route it in the appropriate direction based on your deck's layout. Replace the screws
to reaffix the SBS adapter.