Byonoy Absorbance 96 Automate User manual

Manufacturer:
Byonoy GmbH
Schützenstraße 21
22761 Hamburg, Germany
00 49 40 5379 866 00
[e-mail]
www.byonoy.com
Instructions for Use
Absorbance 96 Automate
Valid for REF Number DE MAA 001
Instructions for Use
Absorbance 96 Automate
Version 1.0 (28/07/2023)
©Copyright 2023 by Byonoy GmbH. All rights reserved.

II
Dear customer,
We are delighted that you have chosen Absorbance 96 Automate. To take full advantage of
the instrument's performance and to enjoy your instrument for many years, please read
these instructions for use carefully before installation and commissioning. Operate the in-
strument in accordance with these instructions. The operating-safety and function of the
instrument can only be guaranteed if both the general safety regulations and accident pre-
vention regulations of the legislator as well as the safety instructions in this manual are ob-
served.
Ensure that the manual is always accessible and is read and understood by all per-
sons operating the instrument.
This user manual may only be used according to its intended purpose. It may not be repro-
duced, changed, or translated in another language without the prior written consent of
Byonoy GmbH.
This document is subject to technical changes and updates.

III
TABLE OF CONTENTS
1 General information....................................................................................................................................1
2 Overview of Absorbance 96 Automate ......................................................................................3
2.1 Scope of application .................................................................................................... 3
2.2 Measurement method ................................................................................................. 3
2.3 Absorbance 96 Automate System ............................................................................ 4
2.4 Consumables .................................................................................................................. 5
3 Preparing the product for use.......................................................................................................... 6
3.1 Unpacking, storage, and transport ........................................................................... 6
3.2 Supplied materials ........................................................................................................ 6
3.3 Working environment .................................................................................................. 7
3.4 Installation ...................................................................................................................... 7
4 Operation ......................................................................................................................................................... 9
4.1 App installation procedure and updates .............................................................. 10
4.2 Computer connection ................................................................................................ 10
4.3 Workflow ....................................................................................................................... 10
5 Signals and troubleshooting.............................................................................................................12
5.1 Signal lights .................................................................................................................. 12
5.2 Troubleshooting ......................................................................................................... 12
6 Maintenance and cleaning.................................................................................................................14
6.1 Maintenance ................................................................................................................ 14
6.2 Cleaning ........................................................................................................................ 15
6.3 Technical support ....................................................................................................... 16
6.4 Repairs .......................................................................................................................... 16
7 Technical specifications.......................................................................................................................17
8 Guarantee .......................................................................................................................................................19
9 Declaration of Conformity................................................................................................................. 20

General information
1
1 General information
Important: Follow the instructions for use
Any commissioning or handling of the instrument requires precise knowledge and notice of
this manual. The device is intended for the described use only.
In this manual and on the label of the reader, particularly important remarks are labeled as
followed:
Symbol
Description
Caution: Caution indicates a potentially hazardous situation, which, if not
avoided, could result in minor or moderate injury.
Information: This is a piece of information indicating certain properties that
must be observed.
CE-mark
Disposal of used electrical and electronic equipment
Manufacturer
Serial number
Consult instruction for use (user manual)
Catalogue number
Liability for function and damage
Liability for the function of the device shall, in any case, pass to the owner or operator if the
device is improperly maintained, repaired, or modified by persons who do not belong to the
authorized service personnel, or if it is handled in a way that does not comply with its in-
tended use. The service and operation of the product must be in accordance with this man-
ual. Byonoy shall not be liable for damages resulting from non-observance of the information

General information
2
above. Warranty and liability conditions of the terms of sale and delivery of Byonoy are not
extended by the information above.
Disposal of used electrical and electronic equipment
The symbol on the product or its packaging indicates that this product is not to be treated
as normal household waste. It must be disposed of at a collection point for the recycling of
electrical and electronic equipment. By contributing to the correct disposal of this product,
you protect the environment and the health of your fellow human beings. Recycling helps to
reduce the consumption of raw materials. For further information on how to recycle this
product, please contact your local authority or municipal waste disposal centres.
Recycling of the product may also be carried out by the manufacturer. Please contact the
service department.
Working with biological and harmful material
The Absorbance 96 Automate is not to be used for the measurement of biohazardous sub-
stances.
Always observe the manufacturer's hazard information pertaining to the substances to be
measured.
The instrument does not produce any toxic or harmful gases or substances. During the
measurement, make sure that there are no toxic or harmful substances in the microtiter plate.

Overview of Absorbance 96 Automate
3
2 Overview of Absorbance 96 Automate
2.1 Scope of application
Absorbance 96 Automate is an optical laboratory instrument for measuring the absorbance
(optical density) of biological or non-biological samples in ANSI/SBS-standard 96-well mi-
crotiter plates in accordance with the specifications described in the user manual. Absorb-
ance 96 Automate is an on-deck microplate reader module designed for easy integration
into various liquid handling or robotic systems.
Absorbance 96 Automate is intended for research and other non-in-vitro-diagnostic anal-
yses only. It is to be operated by trained laboratory personnel and is intended for profes-
sional use.
2.2 Measurement method
Absorbance 96 Automate is designed to carry out sensitive absorbance measurements. It
measures the optical density (OD) of samples at defined wavelengths.
Absorption
Absorption refers to the amount of light absorbed by a medium. Absorption reduces trans-
mission. Transmission is the ratio of incident to transmitted light. Accordingly, the degree of
transmission is calculated as follows
T = (I/I0)
where I is transmitted light and I0is incident light.
Optical Density
Optical density is a measure of the attenuation of light radiation after it has passed through
a medium. Optical density is the logarithmic quantity that describes the reciprocal of the
transmittance T:
OD = log(I0/I)
where I is the transmitted light and I0is incident light.
Optical density is the absorbance of the sample plus other attenuating effects such as scat-
tered light due to turbidity. It is therefore necessary to avoid scattered light in order to meas-
ure absorbance correctly.
If no other attenuating effects are present, absorbance = optical density.

Overview of Absorbance 96 Automate
4
2.3 Absorbance 96 Automate System
Absorbance 96 Automate Reader
Absorbance 96 Automate is an absorbance-based measuring device, i. e. a measuring instru-
ment, with which the absorbance values of a 96-well microtiter plate can be read, recorded
and provided for further processing. Absorbance 96 Automate is a solid-state microplate
reader and has 96 detection units, allowing measurement without a scanning mechanism.
Absorbance 96 Automate is primarily designed to be integrated on-deck of various liquid
handling and other robotic systems.
Figure 1.
Visual representation of the product from the front, top. 1. Illumination unit 2. Signal
light. 3. Groove for the gripper. 4. Detection unit. 5. USB-C cable. 6. SBS adapter. 7. Parking
position. 8. Microplate.
In the illumination unit of the measuring instrument there are up to 6 LED light sources with
different wavelengths and up to 6 interference filters. The signal light positioned at the top
of the illumination unit displays the current status of the reader, including its connection sta-
tus, initialization progress, measurement status, and any errors that may have occurred.

Overview of Absorbance 96 Automate
5
In the detection unit of the reader, 96 photodiodes collect the light passing through the mi-
croplate. The lower and upper parts of the reader are connected via spring contacts.
The microtiter plate is loaded onto the reader using the robotic system's gripper.
On the bottom of Absorbance 96 Automate there is a USB-C port for connecting the instru-
ment to a computer via the included USB-C cable.
Power consumption
Absorbance 96 Automate is an analytical instrument with very low power consumption. It is
operated by a USB C cable and the total power consumption is generally less than 2.5 watts.
Absorbance 96 App
The reader is controlled via the Absorbance 96 App. The operation of the app is described
in-detail in a separate user manual.
2.4 Consumables
With the Absorbance 96 Automate, ANSI/SBS Standard 96-well microtiter plates can be
used. Only certain microplate types can be used with Absorbance 96 Automate (see chapter
7).
When using microtiter plates, always check the specifications of the microtiter plate manu-
facturer. Not all microtiter plates of a particular manufacturer are the same in design, mate-
rials, or configuration. The temperature stability within the microtiter plate can depend on
the type of microtiter plate used.

Preparing the product for use
6
3 Preparing the product for use
3.1 Unpacking, storage, and transport
Unpacking
The device is packed in a specially designed cardboard box. Keep the packaging material. If
the device must be returned for repair, the original packaging material must be used.
Storage and protection during the intervals of normal use
Protect the device from moisture and dust during prolonged storage.
Do not store the device next to heat sources and protect it from direct sunlight.
The temperature for storage should be within the recommended temperature range (see
chapter 7).
Transportation
Before transportation, unplug the instrument and ensure there is no microtiter plate inside
the device. Depending on the transport distance, use the original packaging material. Make
sure that the new location meets the requirements described in chapter 7.
To avoid spillage and contamination, make sure that there is no microtiter plate
inside the instrument before transport.
Always transport the product well-packaged to avoid damage in transit.
3.2 Supplied materials
When unpacking the device, please check that the following components are present:
• Absorbance 96 Automate
o Illumination unit
o Detection unit (with SBS adapter)
o Parking position (with SBS adapter)
• USB-C cable
• Hex key
• Microfiber cloth
• Information card with the download link
• Calibration certificate

Preparing the product for use
7
3.3 Working environment
The Absorbance 96 Automate is designed to be integrated on-deck into liquid handling and
robotic systems.
However, the following warnings must be observed. Ignoring them may lead to measurement
errors and a reduction in the expected lifetime or damage to the device:
Do not operate the device near heat sources or under direct sunlight.
The ambient temperature and the humidity should be within the recommended
range (see chapter 7).
3.4 Installation
Before commissioning Absorbance 96 Automate, you should carefully read and understand
the entire manual to familiarize yourself with the system.
Absorbance 96 Automate is controlled via the Absorbance 96 App, including the analysis of
measurement data. The functions of the app are detailed in a separate user manual.
Commissioning Absorbance 96 Automate on-deck of a liquid handler entails three integra-
tion steps:
1. Placement on-deck
2. Preparing the liquid handler
3. Integrating with the software
Each of these steps are explained below in further detail.
Placement on-deck
Prior to setting up the reader, connect the detection unit to the USB-C cable as depicted in
Figure 2. Remove the SBS adapter by loosening the pair of screws. Insert the USB-C cable
and route it in the appropriate direction based on your deck's layout. Replace the screws
to reaffix the SBS adapter.

Preparing the product for use
8
Figure 2.
Installation of the USB C cable. 1. Detection unit. 2. USB-C cable. 3. SBS adapter. 4.
Screws.
Given that the sensor and parking positions exceed the dimensions of the SBS standard, it
may be beneficial to shift the SBS adapter towards the device's periphery, as illustrated in
Figure 3.
Figure 3.
Possible SBS positions. Bottom view. 1. Sensing or parking position. 2. SBS
adapter.
Position the detection unit and the parking position on any available SBS deck position of
the liquid handler or robotic system. Route the USB-C cable towards a computer or com-
patible device and connect it. Place the illumination unit on top of the detection unit.

Operation
9
Provided that an SBS-outlay is present, the detection unit may be simply placed on-deck:
no further hardware is required to affix the reader.
Consult the specific requirements of your liquid handler for instructions on affixing
hardware on-deck
Preparing the liquid handler
Once Absorbance 96 Automate has been installed on-deck, the gripper of the liquid handling
system must be taught to interact with it.
Consult the manual of your liquid handler for teaching instructions
Labware descriptions may already be available for your liquid handler. Contact us
for more information at [e-mail].
Integrating with the software
Absorbance 96 Automate is controlled via the Absorbance 96 App. The Absorbance 96 App
can connect via SiLA2 to enable remote execution of commands including loading a protocol,
performing a measurement, and exporting the results as a .csv file. Specific Instructions for
Use for the SiLA2 interface can be downloaded from the following webpage:
s.byonoy.com/abs96auto
System-specific drivers may already be available for your liquid handler. Contact
your liquid handler provider for more information, or contact us at se-
[email protected].
4
Operation
The functions of Absorbance 96 Automate are controlled via the Absorbance 96 App, in-
cluding the analysis of measurement data. The functions of the app are detailed in a separate
user manual.

Operation
10
4.1 App installation procedure and updates
The Absorbance 96 app is compatible with Windows und MacOS operating systems (see
chapter 7).
To install Absorbance 96 App, visit the following webpage: s.byonoy.com/abs96. Download
the latest version of the App for your operating system and follow the steps in the Setup
Wizard to complete the installation.
To install the proper drivers and software on the computer, the user must have
administrator rights or obtain such permissions.
4.2 Computer connection
Absorbance 96 Automate requires a connection to the computer for power supply, starting
the experiment, and data transfer. Absorbance 96 Automate starts automatically when con-
nected to a computer via the USB-C cable and the software automatically establishes a con-
nection.
Always use the included Micro-USB cable for the connection to your computer.
Connect the instrument directly to your computer and do not use an external USB
hub.
The device may only be operated at a USB port USB 2/USB 3 with 5 VDC and a
maximum of 3 A of a certificated computer (with certification mark of an approved
testing laboratory). Use an adapter cable on USB 3.1 with type C plug connection,
which ensures the profile 1: 5 [email protected] A.
When Absorbance 96 Automate is switched on, the signal light on the top of the instrument
lights up. Each time the instrument is switched on, an internal self-test is performed to ensure
there are no malfunctions.
4.3 Workflow
Microtiter plate loading
Absorbance 96 Automate is operated using the gripper of the liquid handling or robotic
system. Loading the microplate into the reader involves three steps, which are illustrated in
Figure 4.

Operation
11
Figure 4.
Three steps to load the microplate into the reader utilizing the gripper.
1. Moving the illumination unit to the parking position.
2. Moving the microplate to the detection unit.
3. Moving the illumination unit back to the detection unit.
Assay readout
Once all settings have been completed, the measurement can be started. The app will auto-
matically guide the user through the readout process.
In order to guarantee a correct measurement result, please pay attention to the indications
in the Absorbance 96 App and heed the following warning:
Shocks to Absorbance 96 Automate or the microtiter plate can cause an un-
wanted signal. Therefore, neither the Absorbance 96 Automate nor the microtiter
plate should be touched during measurement.
After a successful measurement, the results section will open automatically in the app.
Removing microtiter plate
The microtiter plate must be removed from the Absorbance 96 Automate after the readout.
To do this, reverse the steps for the loading procedure.
Make sure not to store a microtiter plate filled with reagents in the device, as
evaporating gases can damage the device and especially the filters.

Signals and troubleshooting
12
5 Signals and troubleshooting
5.1 Signal lights
Table 1.
Explanation of the signal lights
Activity of the signal light
Meaning
White, pulsating
Self-test after connection to power.
White, continuous
Device is on and ready for readout.
Colored, continuous
Initialization/measurement in progress.
The color of the signal light indicates the
wavelength of the filter.
Yellow, pulsating
Microplate still in the reader.
Red, blinking
Error
No signal lights light up
Device is off.
Device is defective.
5.2 Troubleshooting
If there is an error, the signal light will blink red. In this case, check the software to see which
error is present.
Table 2.
Error messages and solutions
Type of error
Cause
Solution
Initialization failed.
There was a microtiter
plate in the slot during ini-
tialization.
Remove the plate.
The inside of the slot is
dirty.
Clean the inside of the slot ac-
cording to the cleaning instruc-
tions in chapter 6.2.
Irrecoverable hardware
error.
Contact the service department.
Temperature out of
specification.
The ambient temperature
was too high or too low.
Make sure the ambient tempera-
ture is within the specifications.
USB Power Supply
Defective (< 450mA or
< 4V).
Defective USB port.
Use a different USB port on the
computer or a different computer.
Using a USB hub.
Connect the instrument directly to
the computer.

Signals and troubleshooting
13
Defective Micro-USB ca-
ble.
Contact the service department.
Irrecoverable hard-
ware error.
Irrecoverable hardware
error.
Contact the service department.

Maintenance and
cleaning
14
6 Maintenance and cleaning
6.1 Maintenance
Absorbance 96 Automate is maintenance-free. Each time the instrument is switched on, an
internal self-test is carried out to ensure there are no malfunctions.
There are no parts within Absorbance 96 Automate that can be serviced by the customer. It
is only necessary to ensure that the device is kept clean.
The accuracy, linearity and reproducibility of the device can be checked with a reference
plate. Byonoy recommends the reference plate from Hellma GmbH (item code 666-R013), for
which the following steps are described.
Accuracy
1. Measure the plate at the desired wavelength. Ensure that there is no dust or other
contamination on the reference plate.
2. Compare results taking the measurement uncertainty and specifications of the
reader into account and use the following formula:
ODRP - MU - AS ≤ODRES ≤ODRP + MU + AS
ODRP:Optical density of the reference plate at the desired wavelength. This value is
found on the calibration certificate from the manufacturer of the reference plate.
MU: Measurement uncertainty of the reference plate. This value is found on the cali-
bration certificate from the manufacturer of the reference plate.
AS: Accuracy specification of the reader at measured optical density. This value is
shown in chapter 7.
ODRES: The optical density measured by the reader at the desired wavelength.
Example:
ODRP:1.554
MU: ± 0.0079
AS: ≤1 % + 0.010 OD from 0.0–2.0 OD
ODRES: 1.573
(1) 1.554 - 0.0079 - (1.554*0.001 + 0.010) ≤ODRES ≤1.554 + 0.0079 + (1.554*0.001 +
0.010)
(2) 1.52056 ≤ODRES ≤1.58744
The measured optical density of the reader lies within the calculated range.

Maintenance and
cleaning
15
3. Rotate the reference plate by 180 degrees and repeat steps 1 and 2.
4. If all Results (ODRES) are within the range, the accuracy of the device has been veri-
fied.
Linearity
With the Hellma reference plate, you can check the accuracy at 6 different OD points. If the
accuracy for all these points has been verified, the linearity of the reader is also verified.
Reproducibility
1. Measure the plate at the desired wavelength for at least 3 times. Ensure that there is
no dust or other contamination on the reference plate.
2. Calculate the standard deviation ODSD for each well.
3. Compare the measured standard deviation and specifications of the reader using the
following formula:
ODSD ≤LS
ODSD: Calculated standard deviation for each well
LS: Linearity specification of the reader at measured optical density. This value is
found in chapter 7.
In case that the results of accuracy, linearity or reproducibility are outside the range, please
contact the service department.
There are no recommended intervals for performing accuracy, linearity, and reproducibility
checks. The customer may refer to their own quality control requirements.
Please follow the plate manufacturer’s recalibration instructions for the reference
plate.
6.2 Cleaning
Cleaning the housing
The surfaces of the housing should be cleaned regularly. For this purpose, you can use e. g.
a cloth, or a sponge lightly wetted in water. For heavier soiling, clean the surface of the hous-
ing with a mild soap solution diluted with water or glass cleaner and then wipe with a lightly
moistened cloth or sponge to remove any residue. Do not use scouring agents.

Maintenance and
cleaning
16
Cleaning the shaft
Use a dry microfiber cloth or a bellows to carefully remove dust or dirt from the surface. If
this is not enough, the microfiber cloth can be slightly soaked with 80 % ethanol to carefully
remove dust or contamination from the surface.
Do not allow water or other liquids to get inside the instrument. If this happens, return the
device to the manufacturer for inspection.
Warnings for cleaning
When cleaning, pay attention to the following warnings. Failure to observe these warnings
may result in damage to the instrument. This can lead to a reduction of the service life or in
measurement errors:
Always avoid spraying liquid directly onto the surfaces of Absorbance 96 Auto-
mate. This is especially important for the inside of the shaft, where there are opti-
cal elements that are very sensitive and essential for the functioning of the instru-
ment.
Never clean the inside of the device interior with sharp or abrasive scourges, and
do not use aggressive solvents or corrosive agents.
Biological hazard: Always wear gloves during cleaning operations that may involve
contact with biological or generally hazardous materials or liquids.
6.3 Technical support
In case of problems with the instrument, please contact the manufacturer’s service depart-
ment or your local representative.
Biological hazard: It is your responsibility to decontaminate the instrument and all
accessories before servicing and before returning the instrument or accessories
to the manufacturer.
For decontamination of Absorbance 96 Automate, follow the government guidelines for in-
activation of organisms used in biological laboratories.
6.4 Repairs
Repairs on the device may only be carried out by the manufacturer. Please contact the ser-
vice department. The product warranty is voided if the device is modified by unauthorized
persons, or different parts are installed.

Technical specifications
17
7 Technical specifications
Table 3.
Performance and technical data
Parameter
Value
Type of product
Absorbance reader
Product name
Absorbance 96 Automate
Operating Software
Absorbance 96 App
Service life
10 years at an average use of 4h/day
Housing material
Aluminium
Place for use
Laboratory
Degree of contamination
2
Temperature for storage/measurement
5–40 °C
Temperature (Transport)
-10–50 °C
Relative tolerated humidity (storage/measure-
ment)
Max. 70 %
Measurement method
Absorbance
Measuring technique
Endpoint and Kinetic
Microplate types
96-well, flat bottom
Light source
Up to 6 LEDs
Filters
Up to 6
Detection
96 Photodiodes
Measurement range
0–4.0 OD
Resolution
0.001 OD
Accuracy*
405 nm:
≤1.5 % + 0.010 OD from 0.0–2.0 OD
≤3 % + 0.010 OD from 2.0–3.0 OD
≥450 nm:
≤1 % + 0.010 OD from 0.0–2.0 OD
≤1.5 % + 0.010 OD from 2.0–3.0 OD
Reproducibility**
≤0.5 % + 0.005 OD from 0.0–2.0 OD
≤1 % + 0.010 OD from 2.0–3.0 OD
Linearity***
405 nm:
≤1.5 % from 0,0–2.0 OD!
≤3 % from 2.0–3.0 OD
≥450 nm:
≤1 % from 0,0–2.0 OD
≤1.5 % from 2.0–3.0 OD
Connection to computer
USB 2/USB 3 with 5 VDC and max. 3 A