Campden instruments 7600 Series User manual

INSTRUCTION MANUAL
7600 series Tissue Bath Coolers
for use with all
Campden Instruments Vibrating Microtomes
Campden Instruments UK
PO Box 8148
Loughborough
Leicestershire
LE12 7TJ
Tel: (+44) 01509 817700
Fax: (+44) 01509 81770
Campden Instruments USA
at Lafayette Instrument Co.
PO Box 5729
Lafayette
Indiana 47903
Tel: (+1) 765 423 1505
Fax: (+1) 765 423 4111
www.campdeninstruments.com
March 2006
DCN:
Document Ref: 7600 Eng v1.0
1

EC DECLARATION OF CONFORMITY
Name and address of Manufacturer:
CAMPDEN INSTRUMENTS LIMITED
PO BOX 8148
LOUGHBOROUGH
LE12 7XT
ENGLAND
Description of Machine:
Tissue Bath Cooler
Model number: 7600, 7600S
Serial Number: ............................................
The machine specified above complies with the relevant health and safety requirements of the following:
1. EC Directive(s):
Electromagnetic Compatibility Directive 89/336/EEC
The Low Voltage Directive 73/23/EEC
2. UK Regulations:
Electricity at Work Regulations 1989
3. European Standards
EN 50081-1: 1992 Electromagnetic compatibility generic emissions standard part 1
EN 50082-1: 1992 Electromagnetic compatibility generic immunity standard part 1
Additionally, the health and safety requirements of the following British and harmonised European Standards
have been incorporated in the design of the above machine:
BS 2771:part 1:1986 (EN 60 204: Part1: 1985)
BS 5304:1988
© Campden Instruments Limited 2006
All rights reserved. The information contained in this manual is the property and copyright of Campden
Instruments Limited. No part of this manual may be reproduced in any form or by any means (including
photocopying or storing in any medium by electronic means) without the written permission of the copyright
holder.
The purpose of this manual is to allow the user to achieve expertise in the use of the Instrument and to give the
maintenance technician an insight into maintaining the instrument in peak operating condition.
Please read and understand the information contained in this manual before using the instrument. Only
competent and capable personnel should use the instrument.
This document should be retained for future reference as it contains the name and address of the manufacturer
within the EC
PACKAGING
Please retain the original packaging for future use.
Instruments will not be accepted for service or repair unless the unit has been adequately and properly
packaged. Additionally instruments will not be accepted without prior authorisation and have been certified as
being uncontaminated with any material that may be hazardous to the health of service personnel. Returns
Authorisation and Decontamination Certificate forms can be obtained by contacting Campden Instruments.
1. Introduction
2

1.0 Safety
Spillage - If the cutting lubricant/preserving liquid, e.g. physiological saline, is spilt over the
instrument it is important for electrical safety reasons to ensure that the instrument remains
safe to use. To avoid the possibility of electrical shock if a spillage occurs, the unit should be
switched off at the mains electrical outlet and disconnected before touching the instrument.
The instrument should be inspected and tested if necessary by a suitably qualified technician
before it is put into further use.
This instrument must not be operated unless it is adequately earthed (grounded).
7600 controller with alternative standard and sterilisable cooled tissue baths
All electrical instruments and equipment should be periodically tested to ensure they remain
safe to use. In some countries this may be a statutory requirement. Your local Health and
Safety Executive (or equivalent) will be able to advise on this matter.
1.1 Overview
The 7600 Tissue Bath Cooler Unit is intended for use with the Campden Instruments Range
of vibrating microtomes. Unfixed brain slices sectioned at 4oC give better tissue
preservation and are viable for longer in-vitro recordings. Additionally, some enzyme
histochemical techniques give better staining results when sectioned at low temperatures.
The 7600 series coolers use 'Peltier' thermoelectric elements and are available in two
versions: 7600 (standard tissue bath) and 7600S (sterilisable/autoclavable tissue bath). The
equipment comprises of a mains operated control unit, tissue bath and specimen holder.
The control unit houses a power supply and temperature control circuitry. The standard tissue
bath assembly has a tissue bath with a stainless steel bottom plate, permanently attached to
the cooling elements. The autoclavable specimen bath is manufactured from a special grade
of Nickel-Aluminum Bronze, a material with excellent corrosion resistance properties.
3
This is removable from the cooling elements, which cannot be autoclaved. The cooling

element assembly consists of thermoelectric 'Peltier' elements, temperature feedback sensors
and a cold water fed heat exchanger.
4
7600S Tissue bath with rem
he bath assembly will fit onto any Campden Instruments tissue cutter without modification
urrent from the power supply flows through the thermoelectric elements, which act as heat
. Set-up
.1 Bath Assembly Set-up sembly be connected to an adequate cold water supply whenever
the temperature
ded
ovable, autoclavable piece,
T
and the bath chamber has the same volume as the standard Campden Instruments tissue bath
(752/2B). The tissue holder is the standard Campden Instruments tissue holder (752/2A).
C
transfer units. Heat is drawn off, cooling the solution in the tissue bath. The heat generated by
this process is removed by the water supply fed through the heat exchanger. The unit uses a
PID (proportional integral derivative) temperature control algorithm to maintain temperature
stability. This will hold the bath temperature to within 0.5ºC of the temperature set point.
There will, however, be a small variation in temperature vertically through the bath.
Experience will show the best temperature to be set for any given requirement and ambient
temperature.
2
2
It is essential that the bath as
the unit is in operation. Typically the unit may be connected to a cold-water tap and fed to
waste. Alternatively the water may be pumped through the unit from a suitable reservoir.
The flow of water through the heat exchanger should not be less than 400ml/minute and the
water temperature should be below 25ºC. See figure 1 for connection ports.
A temperature feedback sensor is built into the bath assembly and monitors
of the heat exchanger. The power supply to the thermoelectric elements will shut down if the
water supply is either inadequate or not present. The unit will give an audible ‘beep’ and the
display read ‘Err’ to indicate that the thermoelectric elements have been shut down due to
inadequate flow of cooling water. Switching the unit on and off will reset this condition.
The tissue bath should be filled with physiological buffer solution prepared at the inten
slicing temperature.

Electrical
Inlet
Coolant
Connection Ports
Fig. 1 Tissue bath assembly
2.2 Temperature Controller Set-up
Before connecting the temperature controller to a mains supply, the unit must be set for your
particular voltage supply.
The voltage is set by prising out the fuse holder drawer and re-inserting it such that the
voltage legend for your supply is aligned with the mark on the inlet moulding. See Figure 2.
On/Off switch
Mains socket
Voltage selector &
fuse drawer
Figure 2. Mains inlet/voltage selector (example shown is set to 110-120V)
The inlet moulding accepts a standard IEC socket. Where possible a standard mains lead -
IEC socket/mains plug – suitable for your mains outlet will have been supplied with the
instrument. The instrument must not be operated unless it is connected to a suitably earthed
(grounded) mains supply
Connect the tissue bath to the electrical socket on the rear of the control unit. Switch the controller on
at the rear of the unit.
3. Operation
5

Ensure the cooling water is flowing through the bath before pressing standby to switch on the
control unit. Press the standby key and the LED display will illuminate. The actual
temperature of the tissue bath is displayed. The unit is now operational and power is being
supplied to the tissue bath cooling head. Pressing either the ‘+’ or ‘-‘ keys once will display
the set temperature. Continuing to press the ‘+’ or ‘-‘ keys will change the set temperature.
The displayed temperature will revert back to the actual bath temperature a few seconds after
the last key press.
Standby
If the cooling bath is not connected or has a fault the unit will display ‘Err’. In this situation,
no power is applied to the cooling bath.
The temperature feedback sensor is mounted in the floor of the cooled bath. The temperature
at this point will obviously be different from the temperature at the specimen holder. Over
time this temperature difference will settle to between 1ºC and 2ºC above that of the
measured temperature (depending on conditions).
For the users convenience the set temperature and actual temperature displayed are offset by
1ºC.i.e. A displayed plate temperature of 5ºC will actually be 4ºC.
4. Cleaning & Maintenance
The 7600 Control unit contains no user-serviceable parts and requires no maintenance.
The standard bath assembly requires only cleaning after use. It must not be sterilised by
autoclaving methods nor must it be immersed in water.
The autoclavable specimen bath is manufactured from a special grade of Nickel-Aluminium
Bronze, a material with excellent corrosion resistance properties. The cooling assembly must
not be autoclaved therefore the bath unit must be separated from it before autoclaving. The
bath is secured to the cooling assembly with 4 stainless steel screws M3 x 35 long. When
fitting the bath to the cooling assembly, the underside of the bath should be smeared with
heat-conducting compound (Campden Instruments model 7600-HTP). The bath should be
then ‘wrung’ onto the cooling head to ensure a good heat transfer path and the screws fitted
6

7
and tightened evenly. Do not over-tighten the screws. The heat-conducting compound should
be applied in an even layer sufficiently thick to ensure full area contact. (A thin layer of
compound will transfer heat more efficiently than a thick layer).
The bath may be autoclaved at 140 degrees Celsius for 30 minutes.
All steels (including the so-called stainless steels) will corrode (rust) if left immersed in
physiological saline/a.c.s.f./buffer solutions, the rate of corrosion will increase as the solution
evaporates and the corroding concentrate increases. Stainless steels rely on a thin, protective
oxide layer on their surface to give corrosion resistance. Corrosion occurs when this passive
film breaks down. The main factor causing corrosion is the chloride content of the liquid in
contact with the material; typically seawater, which is generally considered to be quite
corrosive, has a chloride concentration of 19000ppm. A.c.s.f has a much higher
concentration. This concentration will increase due to evaporation and the passive oxide layer
will break down. For this reason it is essential that the bath and heat sink blocks be regularly
and thoroughly cleaned with clean water after use to remove chloride concentrates.
Spillage - If the cutting lubricant/preserving liquid, e.g. physiological saline, is spilt over the
instrument or the Campden vibrating microtome it is important for electrical safety reasons
to ensure that the instrument remains safe to use. To avoid the possibility of electrical shock
if a spillage occurs, the unit should be switched off at the mains electrical outlet and
disconnected before touching the instrument. The instrument should be inspected and tested
if necessary by a suitably qualified technician before it is put into further use.
All electrical instruments and equipment should be periodically tested to ensure they remain
safe to use. In some countries this may be a statutory requirement. Your local Health and
Safety Executive (or equivalent) will be able to advise on this matter.

8
Order codes
Description Order code
Standard Tissue Bath Cooler (complete) 7600
Autoclavable Tissue Bath Cooler (complete) 7600S
Control Unit 7600/1
Standard Tissue bath assembly 7600/2
Autoclavable Tissue bath assembly 7600/3
Autoclavable tissue bath only 7600/4
Heat transfer Paste 765-HTP
Specification
Display Resolution 0.1 ºC
Temperature Accuracy +/- 1 ºC
Temperature Range +8ºC to 0ºC
(Note that the actual temperatures achievable will be dependent upon the solutions
used and local temperature conditions)
Voltage requirements 230V 50Hz or 115V 60Hz
Power Rating 60W
Inlet Fuse Rating T1.25A
For further information contact:
CAMPDEN INSTRUMENTS,
PO BOX 8148
LOUGHBOROUGH,
LEICESTERSHIRE
LE12 7XT.
UK.
Telephone: (+44) 0150 981 4790
Fax: (+44) 0150 981 7701
www.campdeninstruments.com
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