Cantium Scientific MicroBio MB1 User manual

MicroBio Operating Manual
MB1 and MB2 Models

Contents
....................................................................................................................Regulatory Compliance!3
....................................................................................EC DECLARATION OF CONFORMITY!3
.........................................................................................................................................RoHS!3
....................................................................................................................WEEE & Recycling!3
................................................................................................................................Introduction!4
.....................................................................................................................Technical Specification!4
...........................................................................................................................................Warranty!5
..............................................................................................................Notes on using this manual!5
............................................................................................................................Sampler Operation!5
........................................................................................................................Installing Battery!5
.................................................................................................................Inserting a Plate/Dish!6
..................................................................................................................................................MB1!6
.................................................................................................................Switching On and Off!6
..............................................................................................................Setting Sample Volume!6
.........................................................................................................Starting/Stopping Samples!6
..................................................................................................................................................MB2!7
..............................................................................................................................Take Sample!7
.............................................................................................................................Delayed Start!8
..............................................................................................................................User Presets!8
.................................................................................................................................Unit Status!8
..............................................................................................................................Battery Level!8
....................................................................................................................Total Volume Count!9
..................................................................................................................Total Samples Taken!9
................................................................................................................................Unit Version!9
...........................................................................................................Restore Factory Defaults!9
.......................................................................................................................Determining Results!10
.................................................................................................................Change Plate/Dish Type!11
.........................................................................................................................................Cleaning!11
......................................................................................................................................Calibration!12
....................................................................................................................MicroBio Air Sampling!12
..................................................................................................................................Sampling!12
....................................................................................................................................Hygiene!13
..........................................................................................................Temperature & Humidity!13
...................................................................................................................Selection of Media!13
.............................................................................................................................Troubleshooting!14
...........................................................................................................................Technical Support!14
.....................................................................................................................................Appendix A!15
MicroBio Operating Manual [P0001W006]
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Regulatory Compliance
EC DECLARATION OF CONFORMITY
This is to certify that MicroBio MB1 and MB2 products manufactured from January 2006 meet with
the following European Community Directives:
Electromagnetic Compatibility (EMC) Directive 89/336/EEC repealed by 2004/108/EC to the
following standards:
EN 61000-6-3 - Generic Residential & Light Industrial Emissions
EN 61000-6-1 - Generic Residential & Light Industrial Immunity
Signed:
S Plumridge CEng MIET MIEEE FRSA
Managing Director
Cantium Scientific Limited
Clarendon Gardens
Dartford
Kent
DA2 6EY
United Kingdom
as manufacturer of the appliance.
Document Number 09012006/MB1
Document Number 09012006/MB2
RoHS
We have taken the step of ensuring our products meet these requirements as part of our
commitment to manufacturing goods with the least environmental impact.
WEEE & Recycling
Applicable to all EC States only:
At the end of this product's useful life, please dispose at an appropriate recycling collection point
provided in your country, or return to your distributor who is obliged to take the product for safe
recycling and disposal under the European Directive 2003/108/EC.
Outside of the European Community, consult your local regulations or local distributor.
MicroBio Operating Manual [P0001W006]
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Introduction
The MB1 and MB2 MicroBio air samplers are the most economical samplers in the world for
monitoring airborne micro-organisms or bioaerosols.
They have been fully validated by the UK Department of Trade and Industry Validation of Analytical
Methods (VAM) programme to meet the standards required for a reference sampler.
These samplers collect airborne micro-organisms by drawing a stream of air at a constant flow rate
of 100 litres per minute through a series of small holes in a metal head. The air stream then impinges
onto a sterile culture medium in a 55mm contact plate or 90mm Petri dish. After exposure to the air
stream for a fixed period, the contact plate is removed and incubated. The numbers of colonies
which develop are counted, enabling a calculation to be made of the concentration of micro-
organisms in the air (CFU/m3 - colony forming units per cubic metre).
Technical Specification
MicroBio MB1
MicroBio MB2
Flow Rate:!
100 l/min
100 l/min
Sample Volume:
25 to 1,500 litres in 14 preset steps
25 to 10,000 litres in 25 litre steps
Sampling Volume
Capacity:
Up to 30,000 litres before recharge*
Up to 60,000 litres before recharge*
Sample Delay range
-
10 seconds to 3 hours
d50 Particle size
1.7µm (220 x 1mm hole head)
1.7µm (220 x 1mm hole head)
1.35µm (400 x 0.7 mm hole head)
Mean particle velocity
9.62m/s (220 x 1mm hole head)
9.62m/s (220 x 1mm hole head)
10.7m/s (400 x 0.7 mm hole head)
Other Features:
Auto switch off
4-digit 7-seg LED display
Sample cancel feature
Padded carry bag supplied
User programmable preset volumes
Delay start sampling
16 character x 2 line backlit LCD display
Tripod mounting bracket
Padded carry bag supplied
Audible indication of operation/status
Sample cancel with volume taken display
Weight (excluding
charger and carry bag):
650g
700g
Dimensions:!
196 x 100 x 110mm (inc. head)
196 x 100 x 40mm (case only)
196 x 100 x 110mm (inc. head)
196 x 100 x 40mm (case only)
Power:!
4 x AA Cells Alkaline or NiMh
6V at 350mA (maximum)
4 x AA Cells Alkaline or NiMh
6V at 250mA (maximum)
Noise Level:!!
< 75dB @ 1m
< 75dB @ 1m
Environmental Operating
Range:
-10 to 40°C up to 90% RH
-10 to 40°C up to 90% RH
Sampling Plate :
55mm contact plate or 90mm Petri dish
55mm contact plate or 90mm Petri dish
Sampling Head:!!
316 grade stainless steel 220 x 1mm diameter
holes
316 grade stainless steel 220 x 1mm diameter
holes or anodised aluminium 400 x 0.7mm diameter
holes
* Based upon random volume samples at varying intervals until low battery warning given.!These tests were undertaken on
units fitted with new and fully charged Ansmann Max-e NiMh cells.!Actual battery life may vary due to volume taken per
sample, interval between samples, age of cells, and other environmental effects, such as humidity and temperature. Results
obtained from other brands of cells may vary.
MicroBio Operating Manual [P0001W006]
June 2012 4 / 16

Warranty
The manufacturer warrants this product to be free from defects in materials and workmanship for 12
months from the date of purchase.!Your local distributor may offer extended warranties in addition to
this period.
If your product is found to be defective within that period, please contact your local distributor who
will arrange for repair of the instrument, or if necessary a replacement.
This warranty does not cover accidental damage, wear and tear, consequential or incidental loss.
The warranty excludes rechargeable batteries supplied with the sampler. Damage caused by use of
cleaning materials not recommended by the manufacturer will void the warranty.!Any use beyond
the specification of the unit will void the warranty.!Any modifications to the unit without permission or
advice from the manufacturer will void the warranty.
This warranty does not affect your statutory rights.
Notes on using this manual
Operating instructions that follow colour to indicate the following:
RED!:!Control panel button
BLUE!:!Display window message
Sampler Operation
Both MB1 and MB2 models are easy to use hand held instruments.
The MB1 may be hand held or placed on a surface.
The MB2 may be hand held or mounted on a tripod at any height.
Installing Battery
The batteries are held in a small compartment at the back of the MicroBio. The unit is supplied with
4 x AA NiMH high capacity cells. The user can replace these at any time with similar NiMh, NiCd, or
even non-rechargeable Alkaline Batteries. The batteries are removed for re-charging in the "plug in
the wall charger" supplied.
DO NOT ATTEMPT TO RECHARGE ALKALINE BATTERIES using the supplied charger.
When the batteries need re-charging the display will show the words LOW BAT when the unit is first
switched on.
We do NOT recommend the use of Zinc-Carbon Batteries. These can leak and when left in a
discharged state in the battery compartment and can cause corrosion, which can damage the
printed circuit board. We also recommend that batteries are removed from the unit if it is to be left
unused for extended periods to avoid corrosion.
Ensure any cells used are designed for high drain applications.
NOTE: Please read instructions for the supplied charger before charging the NiMh cells.
MicroBio Operating Manual [P0001W006]
June 2012 5 / 16

Inserting a Plate/Dish
For both MB1 and MB2 models, remove the sampling head cover carefully, unless it is being carried
separately in a sterile container. Only hold the edge of the head. Do not touch the front or rear
(inside) surface close to the holes. If necessary the head should be sterilised. Insert a contact plate
inside the springs so that its base sits firmly on the three support posts; then re-fit the sampling
head.
MB1
The MB1 is an extremely simple to use entry level sampler with basic features to get the job done
with the minimum of fuss.
Switching On and Off
To switch the unit on press any of the four switches.!The display will show the last volume setting
used. The unit will switch itself off automatically if not used for 20 seconds.
Setting Sample Volume
To select a volume of air to be sampled use the +and -!buttons to increase or decrease the amount.
The MB1 has fourteen pre-set volumes of: 25, 50, 100, 150, 200, 300, 400, 500, 600, 750, 800, 1,000,
1,200 and 1,500 litres.
Starting/Stopping Samples
Press the START button to start the sample process.!When running, a red LED at the top end of the
sampler will illuminate. If at any point it is required to stop sampling early, press the CANCEL button.
The MB1 will stop sampling when the required volume of air has been sampled.!The red light will
extinguish at the top of the unit.
MicroBio Operating Manual [P0001W006]
June 2012 6 / 16

MB2
The MB2 provides a rich set of features to provide a flexible sampling solution with increased battery
life and sampling capacity.
To switch on, press ON/OFF!once. The LCD display will show the product name followed by the first
menu option of "Take Sample?"!Press ON/OFF!again to switch off the MB2.!The MB2 does not have
an auto power off facility. The MB2 has a simple menu structure to reach all functions.!The options
are accessed by pressing the MENU button and when the menu item required is displayed on the
screen, START is pressed to select it.!
The main menu options are:
"Take Sample?"
This function performs the sample taking of a given volume of air.
"Delayed Start?"
This function adds a user definable time delay before starting a sample.!This gives
the ability to leave the MB2 in a sealed environment to start once the operator has left
the room.
"User Presets?"
The user can define six commonly used volumes of their own to save time in setting up
to take a sample.
"Unit Status?"
This gives information about the MB2 regarding battery, samples taken and version
information.
To take a sample, insert a dish as explained earlier, select a volume of air to sample using “Take
Sample” and proceed to sample the air as described in the following section.
Note:"DO NOT switch off the MB2 when sampling is in progress!
Take Sample
Before setting up to take a sample, remember to have a prepared dish ready in the sampling head.
When Take Sample!is shown on the display,!the user has the option to use one of six preset volumes
or manually set a volume.!The MB2 remembers the last used manual volume. To toggle between
preset or manual mode, use +and -buttons then press START to select the mode.
If in Preset Volume mode, use +and -to select one of the presets. When ready to use that set
volume, simply press START again. Sampling will now start and the fan will run and a red LED will
illuminate at the top of the sampler.
If in Manual Volume mode, use +and -!to set the required volume of air to be sampled.!This may be
between 25 and 10,000 litres of air in increments of 25 litres.! ! Pressing +and -together will set the
volume to 100 litres. When ready to take the sample, press START.
To exit from either manual or preset volume modes before having started a sample, press MENU. At
any stage to cancel sampling, press and hold MENU.
During sampling, the display will show the volume of air taken so far and how long to go, in seconds,
before sampling is complete.
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June 2012 7 / 16

When sampling is complete, the display will show the message SAMPLING DONE!and the final
volume taken. The red LED will flash and a warning tone will be made.!To acknowledge the sample,
press START.
The display will revert back to Take Sample mode.
Delayed Start
The user may wish to undertake sampling remotely.
On some occasions, remote operation of the MB2 is required, such as in sealed clean areas.!To
avoid problems associated with infra red remote controls, i.e. the operator and sampler may not be
in line of sight and windows may not be present, or with wireless types where steel walls may block
transmission, the MB2 employs a time delay start for remote operation.
This allows the operator to set a time between 10 seconds and 3 hours, in variable increments,
before sampling of a user defined volume commences.
When this menu option has been selected, use +and -to change the time delay and press START
when ready. The user can then select the volume required in the same way as in the Take Sample
mode described earlier.
When the sample is started, the delay countdown will begin and the LED will flash slowly, a beep will
sound every second and the display will indicate in how many seconds sampling will start.!Once
sampling has started, MENU can be pressed as normal to cancel.
At any time in setting a delay, press MENU to exit.!Pressing +and -together when setting the delay
will set the factory default delay of 60 seconds.!The MB2 will remember the last used preset delay
for next time, even if the unit has been switched off.
User Presets
Some users find that for almost all the sampling they undertake the volume of air sampled is always
the same. At this menu item, the user can set six possible Preset Volumes which are stored even if
the MicroBio is switched off and on.
When this menu item is first selected, the user is presented with Preset Volume [1]!and the current
set volume.!This value can be adjusted using the +and -"buttons.!When the desired volume is
reached, press the START button to store the value.
The display then moves onto the second preset volume.!If a particular preset is not to be altered,
skip past by pressing START"until the required preset is reached.!When all preset values have been
set and stored, leave this menu option by pressing MENU.
Unit Status
When Unit Status mode is selected, use the +and - keys to navigate between the various screens.!
Pressing START or MENU will take you back to the main menu list.
Battery Level
This display gives a percentage indication of battery capacity. It is only an approximation and
cannot be used to determine the exact operating hours of volume taking capacity remaining in the
battery.
MicroBio Operating Manual [P0001W006]
June 2012 8 / 16

Total Volume Count
This displays the total litres of air the MB2 has taken since the last factory reset. This figure is useful
for the manufacturer’s maintenance and reliability statistics, and may also be used as an indicator for
the user’s regular calibration regime.
Total Samples Taken
This is a count of how many samples have been taken by the MB2 since the last factory reset.
This figure is useful for the manufacturer’s maintenance and reliability statistics and may also be
used as an indicator for the user’s regular calibration regime.
Unit Version
This displays the hardware and software revision information for the MB2.!It is used by the
manufacturer for repair and maintenance purposes.!The software in the MB2 cannot be upgraded
by the user.
Restore Factory Defaults
When Unit Status mode has been selected, it is possible to perform a factory reset on the MB2 by
holding down the +and -buttons simultaneously.!The display will show on the top line ** UNIT
RESET **!with a row of dots appearing on the bottom line.!When sixteen dots appear, the MB2 will
restart itself.
This has the effect of restoring all factory defaults to the pre-set user volumes and zeroing the
samples taken and total volume counts.
MicroBio Operating Manual [P0001W006]
June 2012 9 / 16

Determining Results
Once the sample has been taken, the contact plate or Petri dish should be removed immediately
from the MicroBio and the lid replaced on the plate and sealed.
The plate should then be incubated for a period of time and temperature dependant on the
requirements of the media.!Further information can be found in the Microbiology section of this
manual.
Once incubated, the colony growths are then counted, either manually or using an automatic colony
counter. Due to the statistical nature of the sampling method and the chance more than one colony
impinged at one point on the dish, a count correction needs to be performed.!The table in Appendix
A gives the corresponding corrected value based on the sampling head used on the MicroBio.
Alternatively, the following equation can be used to determine a corrected count.
!!!!!
nc=nf
1.075
1.052 - nf
nh
⎛
⎝
⎜
⎜
⎜
⎜
⎞
⎠
⎟
⎟
⎟
⎟
0.483
Where nh is the number of holes on the sampling head, nfis the number of counted colonies and nc is
the corrected count. If the counted colonies, (nf)exceeds the number of sampling head holes holes
(nh), then the mathematics will fail and the results cannot be trusted. If this is the case, then the
sample dish can be considered as overloaded with organisms and the user should consider lower
sampling volumes.
The colony concentration is then the corrected count, above, per volume of air sampled.!The results
are normally expressed in colony forming units per cubic metre.
To convert the corrected count to CFU/m3use the equation:
CFU
m3=1000. nc
Vs
Where ncis the corrected number of colonies counted and Vsis the sampled volume in litres.
Spreadsheets to automate the correction process and to collate and present results are available for
free download from:
Excel™: http://www.cantiumscientific.com/downloads/PHCA.xls
Apple™ Numbers™: http://www.cantiumscientific.com/downloads/PHCA.numbers
MicroBio Operating Manual [P0001W006]
June 2012 10 / 16

Change Plate/Dish Type
The MicroBio can accommodate both 55mm contact plates or 90mm Petri style dishes. The MB1
and MB2 samplers are factory fitted with the Petri dish springs, but these can be removed and
replaced with contact plate springs (Cantium Scientific Limited part number P0001M007). The
springs for both types have slots to allow a degree of adjustment to suit dish/plate manufacturer
variations.
The picture below shows the fitting of the contact plate springs.
Care must be taken not to over-tighten or cross-thread any of the screws. The tool required is a
small Pozi-head screwdriver (Cantium Scientific Limited part number P0001SCRD). If any screws
are lost, they can be replaced with Cantium Scientific Limited part number P0001SCR003.
Cleaning
The sampling head should be sterilised preferably by autoclave, but in the field disinfect with
suitable sterilising wipes (Cantium Scientific Limited part number 901SW225TY).
In areas of low bioaerosol levels, observe a disinfection regime between each sample. Always
sample from areas of lower contamination towards those of higher contamination. Wash exposed
skin and clothing before sampling and avoid drinking, eating or smoking in the test area. All
equipment should be handled aseptically.
The MicroBio is suitable for use with Hydrogen Peroxide Vapour (H2O2 vapour) bio-decontamination.
Tests have been carried out by Bioquell (UK) Ltd on a range of sensitive electronic equipment to
determine effects of such processes. The conditions of these tests were:
Gas Concentration 1000 ppm
Conditioning time 20 minutes
Gassing time 30 minutes
Aeration time 150 minutes
As the MicroBio has a fan within the sampling head head it is recommended that during the
decontamination process the MicroBio is set to sample at maximum volume so that the H2O2vapour
may be drawn through the head, fan and exhaust for optimum decontamination.
MicroBio Operating Manual [P0001W006]
June 2012 11 / 16

Throughout the trials, Bioquell demonstrated that bio-decontamination with H2O2 vapour does not
appear to be detrimental in any way, effect operational aspects or aesthetics of sensitive electronic
equipment. For further information on Bioquell H2O2 vapour Material Compatibility please refer to
their document BDS-3-MATCOMP-V3.2 available from their website www.bioquell.com or contact
them on [phone].
Calibration
It is recommended that the MicroBio should be regularly calibrated in accordance with specific
industry best practice.!Typically this will be on the anniversary of the instrument entering service.
The only calibration adjustment on the instrument is the flow rate, by default set to 100 litres per
minute.
A validation/calibration kit (Cantium Scientific Limited part number P0059) for the MicroBio MB1 and
MB2 sampler is also available from your local distributor, or the sampler may be returned to your
local distributor or Cantium Scientific Limited.
MicroBio Air Sampling
Although the use of simple settle plates can be used for collection of bacterial and fungal spores, it
can never give a quantitative determination. This passive technique will also fail to enumerate very
small particles such as bacteria, which will remain suspended. The MicroBio MB1 or MB2
bioaerosol samplers allow a quantitative measurement to be made.
Sampling
The selection of the sampling volume is important for reliable sampling. If the contact plate is
overloaded with colonies it is difficult to make an accurate count. With experience, the user will
anticipate the probable bioaerosol concentration in an area, but it may be necessary to make a
preliminary survey at a number of sampling volumes to identify the optimum sampling volume. Each
sample should be repeated several times and a statistical mean value and confidence value for the
value determined.
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Hygiene
The sampling head should be sterilised preferably by autoclave, but in the field disinfect with alcohol
or proprietary wipes. Wipes or alcohol can also be used to sterilise the MicroBio Box. In areas of
low bioaerosol levels, observe a disinfection regime between each sample. Always sample from
areas of lower contamination towards those of higher contamination, wash exposed skin and clothing
before sampling and avoid drinking, eating or smoking in the test area. All equipment should be
handled aseptically.
Temperature & Humidity
Make a note of these values at the time of each measurement. These are important factors in the
likely concentration and continuing viability of airborne microorganisms. For example, some bacteria
survival rates are 35 to 65 times higher at 80%RH compared to 40%RH.
Selection of Media
The agar media used in the 55mm contact plate or 90mm Petri dish should be chosen to suit the
organisms which are being monitored. For a wide range of bacteria, use tryptic soy agar (TSA),
casein soy peptone agar (CPSA) or nutrient agar (NA). There are other selective agars for more
specific micro-organisms. For fungi (yeasts and moulds), use malt extract agar (MEA) or rose
Bengal agar (RBA).
After sampling with the MicroBio Samplers, the agar contact plates are incubated for specified times
and temperatures (typically 1-2 days, at 25 – 37°C) and the colonies which develop are counted. A
correction is applied to the colony count to allow for the possibility that two organisms going through
one sampling hole will result in only one colony growth being observed (positive hole correction).
From the corrected count and the sampling volume used, the number of colony forming units per
cubic metre (CFU/m3) can be determined.
Micro-organism
Agar Culture Medium
Incubation
Temperature
Bacteria:
Bacteria:
Bacteria:
Human Flora
Blood Agar
35 - 37°C
Possible Pathogens
Heart Infusion Agar
Soya bean-casein digest agar
(SCDA)
35 - 37°C
Environmental saprophytic
SCDA or R2A
25 - 30°C
Thermophylic
EMB or Endo Agar
35 - 37°C
Fungi:
Fungi:
Fungi:
Environmental saprophytes
Malt Extract Agar(MEA)
Room Temp
Sabouraud Dextrose
Room Temp
Rose Bengal Agar (RBA)(with
streptomycin), Inhibitory Mould Agar
20 - 25°C
Xerophylic
Malt Extract Agar with added NaCl,
sucrose or dichloran- glycerol
20 - 25°C
Typical media suitable for air sampling
Media selected can be for general detection or selective to assist identification of organisms after
collection. Traditionally Malt Extract and Rose Bengal Agars are used for collection and counts of
fungi. Various recipes are available from suppliers. For bacteria, Tryptic Soy Agar, Casein Soy
Peptone Agar and nutrient Agar are used. For both fungi and bacteria, various chemicals may be
added to the media to give selectivity.
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Troubleshooting
The MicroBio should give many years of trouble-free service with minimal routine maintenance.
However, below are some common questions and answers relating to the use of the MicroBio MB1
and MB2 Air Sampler.!
Q. The unit will not switch on.
A. Check the batteries are inserted correctly and fully charged.!If this does not resolve the
situation, please contact the manufacturer.
Q. (MB1 Only) When setting the volume to be sampled and then pressing start, the fan
seems to run slow and the display dims.
A. This may well be due to exhausted batteries, or use of low quality batteries not supplied by
the manufacturer.!Some AA cells have a higher internal resistance than others, particularly
budget alkaline cells. When the fan starts in the instrument there is a high surge current that will
cause the voltage of the batteries to momentarily dip causing the fan to run slow.!Try using a
new set of high quality alkaline or freshly charged NiMh cells.!The brands of alkaline cells we
have found to work well are Duracell, Energizer and Varta.
Q. The sampler cuts out and switches off during sampling.
A. This is due to exhausted or low quality batteries.!Replace batteries or recharge.
Q. The sampling head fits loosely.
A. There is a retaining spring inside the sampling head area that keeps the head tight.!This may,
with time and use, have moved.!Loosen the screw, slide the spring to the edge of the sampling
head plate and re- tighten.!Try this until a secure fit is obtained.!
Q. The contact plate or Petri dish fit loosely.
A. With time the springs that hold them in place may have loosened. Undo the screws, move the
springs and re-tighten until a secure hold of the plate/dish is obtained.!There is variation in the
outside diameter of plates from one manufacturer to another.!The MicroBio holding springs can
be adjusted to accommodate this variation.
MB2 Only
Q. Unable to adjust properly user pre-set sample volumes or sample volume and samples
records appear to be corrupted.
A. This is a rare event that may possibly happen if the unit is switched off during sampling or just
as sampling completes.!The unit should never be switched off until the user acknowledges
the end of sampling or cancels sampling using the keypad.!If this does occur, select “UNIT
STATUS” from the menu, press START, then hold down the +and -buttons simultaneously
until the display indicates the unit is "RESETTING".!The MB2 will re-boot to factory default
values and clear any problems.
Technical Support
The first point of call for technical support should be your local distributor.!If you do not have a
distributor in your country, please visit our website at http://cantiumscientific.com/Distributors.html.
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Appendix A
Count
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
Corrected
1
2
3
4
5
6
7
8
9
10
11
12
13
14
16
17
18
19
20
21
22
23
24
25
27
28
29
30
31
32
33
35
36
37
38
39
41
42
43
44
Count
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Corrected
45
47
48
49
50
52
53
54
55
57
58
59
61
62
63
65
66
67
69
70
71
73
74
76
77
78
80
81
83
84
86
87
89
90
92
93
95
96
98
99
Count
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
Corrected
101
103
104
106
107
109
111
112
114
116
117
119
121
123
124
126
128
130
132
133
135
137
139
141
143
145
147
149
151
152
155
157
159
161
163
165
167
169
171
173
Count
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
Corrected
176
178
180
182
185
187
189
192
194
197
199
202
204
207
209
212
214
217
220
223
225
228
231
234
237
240
243
246
249
252
255
258
262
265
268
272
275
279
282
286
MicroBio Operating Manual [P0001W006]
June 2012 15 / 16

© Cantium Scientific Limited 2011
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Cantium Scientific Limited
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Dartford
Kent
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United Kingdom
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Fax: [phone]
www.cantiumscientific.com
MicroBio Operating Manual [P0001W006]
June 2012 16 / 16