Carton CN Series User manual

CN Series Microscopes
Instruction Manual

COMPONENTS
1.
2.
14.
10.
11.
12.
13.
DESCRIPTIONS
1. Interpupillary Scale
Adjustable from 48mm through
75mm
2. Eyepieces
WF10x supplied as standard.
WF15x & WF20x are available
options.
3. Left eyepiece tubes with dioptic
adjustment ring
Ajustable for differences in eye
sight.
4. Right eyepiece tube
5. Right and Left Housings
6. Revolving Nosepiece
Inward-faced (reversed) quadruple
revolver accepting objectives
7. Objectives
4x,10x, 40x and 100x provided,
and 40x and 100x retractable and
sealed
Model CN-A
3.
4.
5.
6.
7.
8.
9.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
- 1 -

21. Mechanical stage control knob
Coaxial drive control on right
hand.
22. On-Off switch for illuminator
“I”=On, ”0”=Off
23. Base
With built-in 3 watts white LED
illumination powered by AC/DC
adapter.
24. Light intensity control dial
DESCRIPTIONS
8. Substage Condenser
With iris diaphragm and swing-out
filter holder
9. Swing-out Filter holder
For white diffusion filter provided
10. Locking screw for substage
condenser
11. Lever for iris diaphragm
12. Focusing knob for substage
condenser
13. Illuminator Condenser
14. Viewing head
Siedentopf type with 30°inclined
eyepiece tubes. Trinocular type also
available.
15. Locking thumb screw for head
Make sure to tighten it before use.
16. Stand arm
17. Double layers Mechanical Stage
Travelling through 70mm x 30mm.
18. Tension control ring
For coarse focusing adjustment
19. Coarse focusing adjustment knob
20. Fine focusing adjustment knob
- 2 -

SETTING UP YOUR MICROSCOPE
After opening the styroform container, check that you have the following parts and
accessories.
● Microscope stand complete
● Binocular or Trinocular Viewing head
● WF10x Eyepiece(s) (pair for Binocular or Trinocular head)
● Objectives in plastic container
● White diffusion filter
● AC/DC adapter 100-240VAC 50/60Hrz
● C-Wrench for tension control
● Immersion oil in container
● Dust cover
● Instruction manual
● For trinocular viewing head, C-mount tube and Photo tube
1. Place the stand on a level, stable surface. The microscope is easiest to use
when you face the front of the stage.
2. Loosen the head locking thumb screw provided on the side of the arm, and put
the viewing head onto the receptacle. The head can turn full 360°inside the re-
ceptacle. This permits observation from any direction. Tighten the thumb screw
to fix the head in any position you desire.
3. Put the eyepiece(s) into the eyepiece tube(s), being careful not to touch the
lenses.
4. Take out the objectives from the plastic containers and screw them into the re-
volving nosepiece from the lowest magnification to the higher one in clockwise
direction as you face the upside of the microscope.
5. Turn the switch off(O) and connect the AC/DC adapter to electric power sup-
ply.
C-mount tube
(for trinocular model only)
Photo tube
(for trinocular model only)
AC/DC adapter
Stand
Viewing head
Eyepieces
Objectives
C-wrench
- 3 -

USING AND ADJUSTING YOUR MICROSCOPE
Viewing Head:
The viewing head is inclined 30 degrees to enable comfortable observation and ro-
tates 360 degrees to permit observation from any direction. To rotate it, loosen the
head locking thumb screw and tighten it when the head is located at the position you
desire. Do not use the microscope without tightening the thumb screw. The optical
alignment is adjusted to be used with the thumb screw tightened.
The binocular and trinocular head, require interpupillary adjustment. To adjust the
eyepieces to the interpupillary distance between your eyes, please grasp the right &
left housings below the eyepiece tubes and move them farther apart or closer to-
gether in order to match your pupil distance. The correct adjustment is achieved
when your view becomes comfortable and presents a full single field. Read the in-
terpupillary scale and remember the distance of your pupil for your future use.
The dioptric adjustment ring is located on the left eyepiece tube. To correct a diop-
tric difference between your eyes, begin by looking through the right eyepiece with
your right eye, use the fine focusing handle to get the sharpest image of the speci-
men. Then, look through the left eyepiece with your left eye and turn the dioptric
adjustment ring until you get an image in the same sharpness as the right one. Make
the left eye adjustment without moving the focusing handle.
The CN series has Siedentopf type binocular or trinocular head is designed
to leave the mechanical tube length of 160mm unchanged when the interpupillary
distance is adjusted. This will not affect the diopter adjustment.
Arm:
The arm is fixed securely to the base and supports all the components above the
base. When moving the microscope, please grasp the arm and support the base with
the other hand. Do not carry or hold the microscope any other way.
Base:
Do not open the base. It contains various electrical components.
Illuminator:
Illumination is provided by a 3W LED light powered by AC/DC adapter and con-
denser lens. Turn the switch on (I) and rotate the light intensity control dial to adjust
the brightness.
- 4 -

Objectives:
DIN acrhomatic objectives are corrected for chromatic(colour) aberration. Your
microscopes may be equipped with Semi-plan or Plan achromatic objectives which
minimize the effects of curvature of field and produce flatter images. The 4 objec-
tives will be parfocal when properly fix to the nosepiece so it should not be neces-
sary to make large adjustments in focus when changing objectives.
Objectives 4x and 10x have relatively long working distances while the objectives
40x and 100x have a very short working distance. The 40x and 100x objectives are
retractable (spring-loaded in the lens mount) to protect the specimen slide.
Oil immersion objective 100x is made to get the correct magnification with the in-
termediate of immersion oil between the specimen slide and the objective. When
using 100x objective, begin focusing using the lower magnification objective. Put a
drop of oil on the illuminated area of the specimen and rotate the nosepiece to bring
the 100x objective into the optical path. Looking through the eyepiece, turn the fine
focusing handle to bring the specimen into focus. When bringing the objective into
position, pay attention to not introduce air bubbles into the oil as the bubble will
interfere with your observation. If an air bubble is introduced in to the oil it can eas-
ily be removed by turning the objective to the right and left slightly still touching the
oil on the slide. The air bubble will disappear. After using the 100x objective, be
sure to wipe off the oil thoroughly from the objective and the slide using a soft clean
cloth or lens tissue moistened with Xylol.
The objectives 40xR and 100xR are sealed to prevent damage from contact with
immersion oil, although the oil is not required for use with objective 40xR.
The high power objective requires bright light rays matching the Numerical Aper-
ture of the objective. The NA of the 100x objective is 1.25 and a condenser with
NA1.25 should be used with it. Numerical Aperture(NA) refers to resolving power
limits, and is expressed with formula NA = N x Sin U, N is refractive index of me-
dium (air or oil) between objective and slide, and U is half of the aperture angle.
Larger NA number relates to larger resolving power.
Eyepiece:
One pair of Wide Field eyepieces WF10x are supplied, having Field Number of 20
or 18. The FN20 eyepiece offers extra wide field of view and the high eye point of
18.8mm. The FN18 eyepiece has the eye point of 18.5mm, and a 19mm diameter
micrometer disc can be installed in it as option. The actual field of view of the mag-
nification is calculated as follows.
- 5 -

Field Number of eyepiece
Magnification of objective
So, the larger Field Number of eyepiece makes wider field of view for the objective.
WF15x with retainer for micrometer disc and WF20x without retainer are available
as option.
Mechanical Stage:
The long coaxial drive control mechanical stage is provided and makes the operation
much easier. It runs through 70mmx30mm.
The mechanical stage has vernier scales for measurement of movement in the X-Y
axis. The minimum reading is 0.1mm. By using the vernier you can record the po-
sition of the object on the slide by noting the readings on the scale. Repositioning
the slide to those readings makes locating the object very easy for future observa-
tions.
Focusing Device:
Coaxial coarse and fine focusing adjustment knobs are provided. Turning the coarse
focusing knobs raise the stage until it is stopped by the up stop. Then, looking
through the eyepiece, lower the stage by turning the coarse focusing knobs until you
find the object focused. To get sharper images, use the fine focusing knob. All the
objectives are made PAR-FOCAL. Once focusing is made with one objective, no re
-adjustment of focus by the coarse focusing knobs will be necessary when changing
to any other objective. To make the image sharpest, it may be necessary to make a
slight adjustment using the fine focusing knob.
The fine focus knob is graduated at both sides and each division corresponds to
0.004mm drive and one rotation of the fine focusing knob corresponds to 1.6mm
drive.
The tension control ring is located on the right side of
the coarse focus knob. The tightness of the coarse
focusing movement can be adjusted by turning this
ring using the C-Wrench. For instance, when you
change the sub stage condenser assembly to the phase
turret condenser, if you feel the need to adjust the ten-
sion of the coarse focusing movement, please use this
device. Tension control ring
with C-wrench
- 6 -

Substage Condenser:
Abbe type substage condenser (NA 1.25) focusable with rack and pinion mechanism
is provided. The top lens of the condenser is sealed to prevent damage from contact
with oil. The condenser is replaceable with phase turret condenser. The functions
of the condenser are to bring the light rays to a focus in the plane of the specimen
and to furnish a suitable cone of light to the objective. The diameter of the cone of
light is controlled by the iris diaphragm which provides a variable opening to fill the
back lens of the objective thereby utilizing the full resolving power of the objective.
When the objective 100xR is used, by using the
condenser focusing knob, always move the con-
denser to its highest position. The numerical
aperture 1.25 of the condenser corresponds with
the numerical aperture of the objective 100x.
To induce the full numerical aperture 1.25, it is
designed to be immersed with oil between the
top lens of condenser and the slide, without air
spacing correctly.
Care must be taken to wipe off the oil thoroughly from the condenser lens and the
slide immediately after each use. When the oil immersion objective is used, the con-
denser should always be raised to the highest position.
Iris Diaphragm:
The diaphragm is an important factor in obtaining good images. It is not intended to
control the brightness of the illumination. A large aperture transmits too much light
and creates an image with poor contrast. Smaller apertures increase contrast. To set
the proper aperture, make the aperture of the diaphragm largest and reduce until the
fine details of the specimen are imaged sharply.
Reducing the aperture increases contrast and depth of focus but it also reduces reso-
lution and introduces diffraction. The aperture must be selected for each objective:
i.e. the aperture for the 10x objective (NA 0.25) will not be the same as for the 40x
(NA 0.65), since the angle of light required is determined by the numerical aperture
of the objective. Proper adjustment of the diaphragm aperture is easily determined.
After placing an objective into the optical path and focusing, remove the eyepiece
and look through the optical tube. Observe the diaphragm through the objective,
then move the iris lever to coincide with the edge of the objective aperture.
- 7 -

MAINTENANCE OF MICROSCOPE
Model CN series microscope is designed such that only a minimum maintenance
work is necessary. Nevertheless proper care will extend the life of the instrument
considerably. There are five important rules you should observe.
1. Keep it away from dust. Always cover the microscope with the vinyl dust cover
provided, or put it back to the styrofoam container, whenever you are not using the
instrument. If dust gets on the lenses, blow it off with a rubber syringe or use a
camel's hair brush. Optical lenses are very soft, and can easily get scratched by
dust particles.
2. Do not touch the lens. Do not touch the lenses, finger print are difficult to remove.
Use a soft linen moistened with xylol to remove fingerprints.
3. Keep it away from moisture. Moisture is optical lenses worst enemy. Once you
get fungus on the lenses, they cannot be returned to their original condition. Keep
the instrument in a dry place.
4. Do not drop the microscope. Needless to mention, optical parts are very delicately
made and are easily broken. Also the mechanical parts are made with the highest
precision and will be damaged if dropped.
5. Do not dismantle. Fitting of all parts have been done with great care by skilled
experts, and once you dismantle, the smoothness of mechanical part or clearness of
optics will be spoiled. Return the instrument to an authorized dealer or to the
factory if need for dismantling arises.
Swing-out Filter Holder:
A filter holder is located under the iris diaphragm and a white diffusion filter is sup-
plied. Swing out the filter holder, insert the filter and swing it back to position.
The white diffusion filter diffuses and weakens the light. Other filters of 32mm dia.
can be used for different purposes if necessary.
- 8 -

PHASE CONTRAST SYSTEM
Phase contrast is a form of illumination which alters the normal path of light waves
from an object to a wavelength shifted diffraction pattern thus allowing visualization
of transparent or low contrast specimens. This allows living cells and suspensions to
be viewed without the need for staining procedures.
● Phase Set Components
● Phase Turret Condenser, N.A 1.25
● Achromat or Plan Phase Objectives (DM)
10X NA=0.25
20X NA=0.40
40X NA=0.65
100X (oil) NA=1.25
● Centering Telescope
● Green Filter
● Installation Key (Screwdriver)
● Storage Case
INSTALLATION AND ALIGNMENT
1. Replace the normal brightfield objectives of the microscope with the phase ob-
jectives (Dark & Medium type) supplied in the storage case.
2. Remove the brightfield substage condenser by loosening the condenser locking
thumb screw and gently lowering the condenser down and out of the mounting
ring. This thumb screw is located on the left side of the condenser mount as
you face the front of the microscope.
3. Install the Phase Turret Condenser by sliding it up into the condenser mount
and tightening the condenser locking thumb screw using the Installation Key
provided. The index mark (white dot) on the turret condenser should be situ-
ated at the front of the microscope.
4. Rotate the turret dial of the condenser to align the zero (0) mark to the index
mark on the turret housing. This is the setting to use for any brightfield work.
5. Raise the condenser to its maximum height and place a specimen slide on the
stage.
6. Swing the 10X objective into the optical path and focus.
7. Move the slide, using the mechanical stage, to a location with little or nothing
in the field of view.
8. Rotate the turret dial of the condenser to align the 10 mark to the index mark
(white dot) on the turret housing.
9. Replace either eyepiece with the Centering Telescope. Looking through the
telescope, you can see two rings of light, one at the center of view, the other
slightly off center.
- 9 -

10. Using the focusing control of the Centering Telescope only, focus the rings to
maximum clearness.
11. To achieve optimum performance, the bright ring must be positioned concen-
tric with the darker ring at the center of view. This is done by using the knurled
ring holding the illumination annulus located underneath the turret housing.
Move the knurled ring until the bright ring is concentric with the dark ring.
(See the right chart)
12. Put the green filter onto the top of the illuminator condenser frame.
13. Replace Centering Telescope with normal eyepiece. You can observe an excel-
lent phase contrast view of your specimen.
14. STEP 9-12 should be performed for the other objective lenses.
Note)
1: The phase turret dial number must match the magnification power of each
objective. 10=10X, 20=20X, 40=40X, 100=100X
2: Use slides which are free of dust and 1mm thick with cover slips 0.17mm
thick.
3: Care should be taken not to disturb the illumination annulus under the tur-
ret of the phase condenser after it has been adjusted (STEP 11), otherwise
misalignment may occur.
- 10 -

TV MICROSCOPY
Direct Focus
Trinocular model provides the C-Mount tube as a standard accessory. Through the
C-mount tube, the image produced by objective can be sent directly to CCTV cam-
era. Compared with the secondary image produced with the TVA-45 camera
adapter, the direct image becomes sharper and clearer, while the field of view be-
comes narrower.
How to mount CCTV camera onto micro-
scope
1. Make sure that your CCTV camera has
C-mount.
2. Separate the C-mount tube into two
pieces by loosening the locking thumb
screw, black short tube and long white
tube.
3. Attach the camera to the short tube.
4. Screw the long tube into the viewing
head.
5. Attach the short tube with the camera to
the long tube and tighten the locking
thumb screw.
6. Connect the camera to monitor by fol-
lowing the instructions provided with the
camera and the monitor.
If necessary, you can change the direction of the image on the monitor by turning
the black short tube with the camera after loosening the locking thumb screw.
Make sure to tighten the said locking thumb screws after adjustment.
Camera
Short tube
with C-mount
Locking thumb
screw
Long tube
- 11 -

Indirect Focus
Model TVA-45 adapter is optionally available, allowing a CCTV camera to be con-
nected with photo tube of microscope. The TVA-45 adapter has a built-in reducing
lens to make the field of view wider and also make the magnification smaller than
the direct focused image.
How to mount CCTV camera with the TVA-
45 adapter
1. Screw the photo tube into the viewing
head.
2. Attach the camera to the adapter.
3. Attach the adapter with the camera to the
photo tube.
4. Connect the camera to monitor by fol-
lowing the instructions provided with the
camera and the monitor.
Camera
TVA-45
Photo tube
- 12 -

PHOTOMICROGRAPHY WITH DIGITAL STILL CAMERA
You can take photomicrographs with your SLR digital camera using optional
accessories Model DG adapter and various kinds of T-mount adapter available
according to your camera.
How to mount SRL digital camera onto microscope
1. Remove the mounted lens from your camera.
2. Attach a T-mount adapter to the DG adapter.
3. Mount your camera body to the T-mount adapter
with the adapter.
4. Put the DG adapter with the camera body into
the photo tube.
5. Turn the camera to the position you desire and
tighten the locking screw of the DG adapter.
6. Put the specimen into focus looking through
eyepieces, and then, focus it sharper looking
through the camera finder if necessary. T-mount adapter
T-mount
adapter
DG
- 13 -

OPTIONAL PARTS AND ACCESSORIES
Heads:
CNA471 Trinocular head, Siedentopf type, w/photo tube and C mount tube,
without eyepiece
CNA472 Binocular head, Siedentopf type, without eyepiece
CNA473 Monocular head, without eyepiece
Eyepieces:
M9261-100 DIN Wide Field 10x FN18 , with retainer for reticle
M9261-101 DIN Extra Wide Field 10x FN20
M9261-150 DIN Wide Field 10x, with retainer for reticle
M9261-200 DIN Wide Field 20x
Objective set:
CDA520 DIN Semi-plan achromat 4x,10x, 40xR(sealed), 100xR oil
CDA530 DIN Plan achromat 4x, 10x, 40xR(sealed), 100xR oil
Miscellaneous:
M9260-1 Phase contrast accessory with phase achromat 10x, 20x, 100xR oil &
phase plan 40xR, phase turret condenser, centering telescope, green
filter, driver in aluminum case
CDA550 Phase contrast accessory with phase plan 10x, 20x, 40xR, 100xR oil,
phase turret condenser, centering telescope, green filter, driver in
aluminum case
CDA551 Dark field condenser with Plan objective 100x with iris diaphragm
M9260-2 Simple polarizing set
DG Adapter for digital SRL camera
T-mount adapters according to digital SRL cameras
- 14 -

CN 110311
STANDARD SPECIFICATIONS
Model Head Eyepieces Objectives Condenser Stage Illuminator
CN-SP Siedentopf
binocular WF10X(pair)
DIN Semi Plan Achromat
4x,10x,40xR(sealed),
100xR Oil
Sealed NA1.25
Abbe, Rack &
Pinion
Mechanical
stage
3W LED with
Dimmer
CN-P Siedentopf
binocular WF10X(pair)
DIN Plan Achromat
4x,10x,40xR(sealed),
100xR Oil
Sealed NA1.25
Abbe, Rack &
Pinion
Mechanical
stage
3W LED with
Dimmer
CN-PH Siedentopf
binocular WF10X(pair)
DIN Plan Phase 10x,
20x,40xR(sealed),
100xR Oil
Sealed NA1.25
Abbe, Rack &
Pinion w/Phase
turret
Mechanical
stage
3W LED with
Dimmer
CN-A Siedentopf
binocular WF10x(pair) DIN Achromat
4x,10x,40xR, 100XR Oil
Sealed NA1.25
Abbe, Rack &
Pinion
Mechanical
stage
3W LED with
Dimmer
Carton Optical Industries, Ltd.
27-11 Higashi Ueno 1-chome,
Taito-ku, Tokyo 110-0015, Japan
Email: [email protected]