Carton CS series User manual

Advanced Microscopes
Advanced MicroscopesAdvanced Microscopes
Advanced Microscopes
Model CS series
Model CS seriesModel CS series
Model CS series
Instruction Manual
Instruction ManualInstruction Manual
Instruction Manual

COMPONENTS
COMPONENTSCOMPONENTS
COMPONENTS
Model CST-10
Model CST-10Model CST-10
Model CST-10
11
28
13
12
14
13
1
14
2
15
3
16
17
4
18
5
19
6
20
7
21
8
22
9
23
10
24
25
26
27
-2-

GENERAL GUIDE
GENERAL GUIDEGENERAL GUIDE
GENERAL GUIDE
This tube can adopt a normal eyepiece
1.Interpupillary scale
1.Interpupillary scale1.Interpupillary scale
1.Interpupillary scale
for teachin or trainin purpose.
Interpupillary distance adjustable
from 48mm throu h 75mm.
13.Locking thumb screw
13.Locking thumb screw 13.Locking thumb screw
13.Locking thumb screw
for Photo tube
for Photo tube for Photo tube
for Photo tube
2.Eyepiece
2.Eyepiece2.Eyepiece
2.Eyepiece
Adjustable parfocal between
WF10x(FN=18) supplied as standard.
the vertical photo tube & the binocular.
WF15x & WF20x are available as option.
15.Locking thumb screw
15.Locking thumb screw 15.Locking thumb screw
15.Locking thumb screw
3.Dioptric a justment ring
3.Dioptric a justment ring3.Dioptric a justment ring
3.Dioptric a justment ring
for Vertical tube
for Vertical tube for Vertical tube
for Vertical tube
Adjustable a dioptric difference a ainst
Adjustable a view an le of monit
o
r ima e.
ri ht eye.
Provided on the left eyepiece tube.
16.Trinocular viewing hea
16.Trinocular viewing hea16.Trinocular viewing hea
16.Trinocular viewing hea
4.Binocular/Sie entopf type
4.Binocular/Sie entopf type 4.Binocular/Sie entopf type
4.Binocular/Sie entopf type
17.Sli ing knob
17.Sli ing knob17.Sli ing knob
17.Sli ing knob
With 30°inclined eyepiece tubes.
When the slidin knob is pulled out,
When adjustin interpupillary distance,
80% of the li ht comes to the photo tube
please rasp the ri ht & left housin s
and 20% to the binoculer tubes.
instead of eyepiece tubes.
18.Locking thumb screw
18.Locking thumb screw 18.Locking thumb screw
18.Locking thumb screw
5.Revolving Nosepiece
5.Revolving Nosepiece5.Revolving Nosepiece
5.Revolving Nosepiece
for Hea
for Hea for Hea
for Hea
Inward-facin (reversed) Quadruple
360°rotatable Monocular, Binocular or
Nosepiece on ball bearin mount with
Trinocular head.
click-stop mechanism for smooth and
Please use after ti htenin .
accurate operaton. Quintuple nosepiece
is also available as option.
19.Arm
19.Arm19.Arm
19.Arm
6.Objectives
6.Objectives6.Objectives
6.Objectives
20.Double layers Mechanical Stage
20.Double layers Mechanical Stage20.Double layers Mechanical Stage
20.Double layers Mechanical Stage
DIN Achromatic Objectives are provided.
141x131mm, travellin throu h 76x51mm.
Semi-plan & Plan objectives are also
2 slides can be held at a time for
available as option. 40x & 100x objectives
comparison viewin .
are sprin -loaded.
21.Tension control ring
21.Tension control ring21.Tension control ring
21.Tension control ring
7.Substage Con enser
7.Substage Con enser7.Substage Con enser
7.Substage Con enser
The focusin movement torque can be
Abbe type NA=1.25 Condenser with iris
adjusted by turnin the tension control
diaphra m & swin -out filter holder.
rin usin the C-wrench.
Replaceable with Phase Turret
Condenser as option.
22.Coarse & 23.Fine Focusing han le
22.Coarse & 23.Fine Focusing han le22.Coarse & 23.Fine Focusing han le
22.Coarse & 23.Fine Focusing han le
Coaxial focusin adjustment devices.
8.Swing-out Filter hol er
8.Swing-out Filter hol er8.Swing-out Filter hol er
8.Swing-out Filter hol er
25mm Coarse focusin ran e, and
White & Blue filters are provided.
0.002mm fine focusin increments.
9.Mechanical stage control han le
9.Mechanical stage control han le9.Mechanical stage control han le
9.Mechanical stage control han le
24.Focusing han le
24.Focusing han le24.Focusing han le
24.Focusing han le
Coaxial drive handles on left hand.
for Sub stage Con enser assembly
for Sub stage Con enser assembly for Sub stage Con enser assembly
for Sub stage Con enser assembly
Focusable by rack and pinion.
10.Illuminator Con enser
10.Illuminator Con enser10.Illuminator Con enser
10.Illuminator Con enser
6v20w Halo en lamp.
25.Base
25.Base25.Base
25.Base
11.Photo tube cap
11.Photo tube cap11.Photo tube cap
11.Photo tube cap
26.On-Off switch for illuminator
26.On-Off switch for illuminator26.On-Off switch for illuminator
26.On-Off switch for illuminator
This cap should be put onto the photo
"I"=On,"0"=Off.
tube to prevent from dust when not usin .
27.Light intensity control
27.Light intensity control27.Light intensity control
27.Light intensity control
12.Photo tube & 14.Vertical tube
12.Photo tube & 14.Vertical tube12.Photo tube & 14.Vertical tube
12.Photo tube & 14.Vertical tube
This tube is used for photomicro raph
28.C-Mount tube & 14.Vertical tube
28.C-Mount tube & 14.Vertical tube28.C-Mount tube & 14.Vertical tube
28.C-Mount tube & 14.Vertical tube
or viewin throu h a monitor.
replaceable as standard.
-3-

SETTING UP YOUR MICROSCOPE
SETTING UP YOUR MICROSCOPESETTING UP YOUR MICROSCOPE
SETTING UP YOUR MICROSCOPE
After openin the styroform container, check that you have the followin parts
and accessories.
●Microscope stand complete
●Monocular, Binocular or Trinocular Viewin head
●WF10x Eyepiece(s) (pair for Binocular or Trinocular head)
●Objectives in plastic container
●White & Blue filters (1pce.each) and 2pcs. spare Fuses
●Spare 6v20w halo en lamp in carton box
●Electric power supply cord (Inlet type)
●C-Wrench for tension control
●Immersion oil in container
●Dust cover
●Instruction manual
In case of Trinocular viewin head
●Photo tube with strai ht tube & C-Mount tube with strai ht tube
1) Place the stand on a level, stable surface.
The stand is easiest to use when the stand is situated with the sta e towards you.
2) Loosen the head lockin thumb screw provided on the side of the arm, and put
the viewin head onto the receptacle. The head can turn full 360°inside the
receptacle. This permits observation from any direction.
Ti hten the thumb screw to fix the head in any position you desire.
3) Put the eyepiece(s) into the eyepiece tube(s), bein careful not to touch
the lenses.
4) Take out the objectives from the plastic containers and screw them into the
revolvin nosepiece from the lowest ma nification to the hi her one in clockwise
direction as you face the upside of the microscope.
5)Turn the switch off(O) and connect the electric power supply cord.
Model CST-10
-4-

USING AND ADJUSTING YOUR MICROSCOPE
USING AND ADJUSTING YOUR MICROSCOPEUSING AND ADJUSTING YOUR MICROSCOPE
USING AND ADJUSTING YOUR MICROSCOPE
Viewing Head:
Viewing Head:Viewing Head:
Viewing Head:
The viewin head is inclined 30 de rees to enable comfortable observation and
360 de rees to permit observation from any direction. To rotate it, loosen the
head lockin thumb screw and ti hten it when the head is located at the position
you desire. Do not use the microscope without ti htenin the thumb screw,
as the optical ali nment is adjusted to be used with the thumb screw ti htened.
On the binicular or trinocular head, interpupillary adjustment are required.
To adjust the eyepieces to the interpupillary distance between your eyes,
please rasp the ri ht & left housin s instead of eyepiece tubes, and move them
to ether farther apart or closer in order to match your pupil distance.
Adjustment is proper when the field becomes comfortable and presents a full
sin le field. Read the interpupillary scale and remember the distance of your pupil
for your future use.
The binocular head mechanism of this model is
Sie entopf
Sie entopfSie entopf
Sie entopf
type, and
the mechanical tube len th is not chan ed to any position. (MTL.=160mm)
On the left eyepiece tube, the dioptric adjustment rin is provided. When there
is a dioptric difference between your eyes, while lookin throu h the ri ht eyepiece
with your ri ht eye, use the fine focusin handle to et the sharpest ima e of the
specimen. Then, look throu h the left eyepiece with your left eye and turn the
dioptric adjustment rin until you et an ima e in the same sharpness as the ri ht
one. Make the left eye adjustment without movin the focusin handle.
Arm:
Arm:Arm:
Arm:
The arm is fixed securely to the base and supports all the components above
the base. When movin the microscope, please rasp the arm and support the
bottom surface by the other hand. As the other parts are made delicately,
don't hold them for movin or carryin with.
Base:
Base:Base:
Base:
The base supports all of instrument, and all electric parts are contained in it.
Don't open the base without attendance of an en ineer.
Illuminator:
Illuminator:Illuminator:
Illuminator:
Illuminator is composed of the 6v20w halo en lamp and the condenser lens.
Turn the switch ON(I) and rotate the li ht intensity control and set the
bri htness properly.
Obje tives:
Obje tives:Obje tives:
Obje tives:
Achromatic objectives supplied are corrected from chromatic(colour) aberration.
The 3 or 4 objectives fixed correctly to the revolvin nosepiece are parfocal,
so, it should not be necessary to make lar e adjustments in focus when chan in
objectives.
-5-

Objectives 4x and 10x have rather lon workin distance and focus at some
distance to the specimen slide. The objective 40x and 100x have rather than short
workin distance and the focused distance to the specimen is very short.
So thay are provided with sprin in the lens mount to protect the specimen slide
from erroneously breaka e. Oil immersion objective 100x is made to et the
correct ma nification with the intermediate of immersion oil between the
specimen slide and the objective.
Numerical Aperture & Workin Distance
Achromatic 4ｘ： ＮＡ＝0.10， ＷＤ＝18.3ｍｍ
Achromatic 10ｘ： ＮＡ＝0.25， ＷＤ＝6.96ｍｍ
SP 40ｘ ： ＮＡ＝0.65， ＷＤ＝
0.35
ｍｍ
Achromatic100ｘ： ＮＡ＝1.25， ＷＤ＝0.148ｍｍ
When usin 100x objective, secure the focus by usin the lower ma nification
objective. Put a drop of the oil on the illuminated area of the specimen and
rotate the nosepiece to brin the 100x objective into the optical path.
Lookin throu h the eyepiece, turn the fine focusin handle to brin the
specimen into focus. When brin in the objective into position, pay attention
not to catch air bubble in the oil as the bubble disturbs observation.
The air bubble can be observed clearly, if you look throu h from eyepieace tube
removin the eyepiece. When air bubble is in the oil, turn the objective to ri ht
and left sli htly still touchin the oil on the slide. Then air bubble will disappear.
After usin the 100x objective, be sure to wipe off the oil thorou hly from the
objective and the slide usin a soft clean cloth or lens tissue moistened with
Xylol.
Also the hi h power objective requires bri ht li ht rays matchin the Numerical
Aperture of the objective. The NA of the 100x objective is 1.25 and condenser
should be used with it. Numerical Aperture(NA) refers to resolvin power limits,
and is expressed with formula
NA=NxSinU
NA=NxSinUNA=NxSinU
NA=NxSinU
, N is refractive index of medium(air
or oil) between objective and slide, and U is half of the aperture an le.
Lar er NA number relates to lar er resolvin power.
Achromat
ic
20x,60xR and Semi Plan 4x,10x,40xR,100xR and Plan4x,10x,40xR,
100xR are available as option.
Eyepie e:
Eyepie e:Eyepie e:
Eyepie e:
One pair of Wide Field eyepieces W
F
10x are supplied
(monoculer head:1piece)
,
havin Field Number of 18
and hi h
eyepoint of 1
8.5
mm.
They can be installed
with micrometer disc of 19mm dia.
The actual field of view of the ma nification is calculated as follows.
Fiel Number of eyepiece
Fiel Number of eyepiece Fiel Number of eyepiece
Fiel Number of eyepiece
Magnification of objective
Magnification of objective Magnification of objective
Magnification of objective
So
,
the bi er
F
ield
N
umber of eyepiece makes wider field of view for the objective.
WF15x with retainer for reticle and WF20x without retainer are available as option.
= Fiel of view (mm)
= Fiel of view (mm)= Fiel of view (mm)
= Fiel of view (mm)
-6-

Me hani al Stage:
Me hani al Stage:Me hani al Stage:
Me hani al Stage:
Coaxial drive control mechanical sta e is provided. It runs throu h 51mm x 7
6
mm
and can cover the full 2"x
3"slide or 2 pieces of 1"x 3"slide to ether.
The lon vertical
coaxial drive control makes the operation much easy.
The mechanical sta e has scales for the cross way movements of
X
and
Y directions
.
The minimum readin is 0.1mm by usin the vernier and you can
measure the rou h
s
ize of the object.
Fo using Devi e:
Fo using Devi e:Fo using Devi e:
Fo using Devi e:
Coaxial coarse and fine focusin adjustment
handles
are provided.
Turnin the coarse focusin
handle
, raise the sta e until it is stopped by the up stop.
Then
,
lookin throu h the eyepiece, lower the sta e by turnin the coarse focusin
handle
until you find the object focused. To et sharper ima es, use the fine focusin
handle
.
All the objectives are made PAR-FOCAL. Once focusin is made with one
objective,
no re-adjustment of focus by the coarse focusin
handle
will be necessary
when chan in
to any other objective. To make the ima e sharpest, it may be
necessary to make a sli ht adjustment by the fine focusin
handle
.
The fine focusin
handle
is
raduated
at both sides
and each division corresponds to
0.002mm
drive and one rotation of the fine focusin
handle
corresponds to 0.
20
mm
drive.
On the ri ht shaft of the coarse focusin
handle
,
the tension control rin is provided.
The ti htness of the coarse focusin movement
can be adjusted by turnin th
is
rin
usin the
C-Wrench. For instance, when you chan e
the sub sta e condenser assembly to the phase
turret condenser, if you feel need to adjust the
tension of the coarse focusin movement,
please use this
device
.
Tension control rin with C-Wrench
Substage Condenser:
Substage Condenser:Substage Condenser:
Substage Condenser:
Abbe type substa e condenser
(
N.A.1.25
)
is provided. The functions of the
condenser are to brin the li ht rays to a focus in the plane of the specimen and
to furnish a suitable cone of li ht to the objective. The diameter of the cone of
li ht is control
l
ed also by the
iris diaphra m provin a continuously variable increase
and reduction and should fill the
back lens of the objective to utilize the full
resolvin power of the objective.
-7-

Lockin thumb screw
for Condenser
Iris lever
Filter holder lever
for swin -out
Focusin Handle
for
Substa e Condenser
The numerical aperture 1.25 of the condenser corresponds with the numerical
aperture
of the objective 100x. To induce the full numerical aperture 1.25,
it is desi ned to be
immersed with oil between the
top lens of
condenser
and the slide, without air spacin correctly.
However, it is often used without oil on the top lens of the condenser, because
microscopic specimens enerally are low in contrast and the numerical aperture
of the condenser is reduced to about 70% which makes better contrast.
When the condenser
is used with immersion oil and utilized with the full cone of
li ht, the adjustment of the
aperture can be made by the iris diaphra m.
Care must be taken to wipe off the oil thorou hly from the condenser lens and
the slide
immediately after each use. When oil immersion objective is used,
the condenser should
be used at the hi htest position always.
Iris Diaphragm:
Iris Diaphragm:Iris Diaphragm:
Iris Diaphragm:
The diaphra m is an important factor in obtainin ood ima es.
It is not intended to
control the bri htness of the illumination, and diffract li ht
rays for inducin proper contrast
in the specimen.
Too much li ht makes the ima e bad, because it decreases the contrast and
smaller aperture increases contrast in the ima e. To et the proper aperture,
make the aperture
of the diaphra m lar est and reduce until the fine details of
the specimen are ima ed
sharply.
Reducin the aperture does increase contrast and depth of focus but also reduce
resolution and introduces diffraction. The aperture must be selected for each
objective:
i.e. the aperture for the 10x objective (N.A.0.25) will not be the same
as for the 40x
(N.A.0.65), since the an le of li ht required is determined by the
numerical aperture of
the objective. Proper adjustment of the diaphra m aperture
is easily determined
. After one objective into the optical path and focus,
you look throu h from the eyepiece tube removin the eyepiece, you can observe
the diaphra m throu h the objective, please move the iris lever and coincide with
the objective aperture.
-8-

Swing-out Filter Holder:
Swing-out Filter Holder:Swing-out Filter Holder:
Swing-out Filter Holder:
A filter holder is provided under the iris diaphra m and white and blue filters are
supplied. Swin out
the filter holder, insert the filter and swin it back to position.
The blue filter absorbs some of the excess red and yellow from the li ht source
and for stained specimens it renders the stains closer to their appearance in
dayli ht.
The white filter diffuse and weaken the li ht.
Other different filters can be adopted for different purposes.(φ32mm)
MAINTENANCE OF MICROSCOPE
MAINTENANCE OF MICROSCOPEMAINTENANCE OF MICROSCOPE
MAINTENANCE OF MICROSCOPE
Model C
S
series microscope is desi ned such that only a minimum maintenance
work is
necessary. Neverthless proper care will extend the life of the instrument
considerably.
There are five important rules you should observe.
(1) Keep it away from dust. Always cover the microscope with vinyl dust cover,
or put it
back to the styrofoam container, whenever you are not usin the
instrument. If dust
ets on the lenses, blow off with rubber syrin e or
use camel's hair brush. Optical lenses
are very soft, and can easily et
scratched by dust particles.
(2) Do not touch the lens. Fin erprints are very difficult to remove, that try
not to put
fin erprints on the lenses. However, if you do, use washed out
soft linen moistened
with xylol to remove t
hem
.
(3) Keep it away from moisture even thou h anti-mould treated optics.
Moisture is the worst enemy to the optical lenses.
Once you et fun us on the
lenses, it cannot be returned to the ori inal condition.
Keep the instrument in a
dry place and store it with desiccant such as Silica-Gel.
(4) Do not drop the microscope. Needless to mention, optical parts are very
delicately
made and may et broken by droppin . Also the mechanical parts
are made with the
hi hest precision and obviously will be dama ed if dropped
on the floor.
(5) Do not dismantle. Fittin of all parts have been done with reat care by
skilled experts,
and once you dismantle, the smoothness of mechanical part or
clearness of optics will be
spoiled.
Brin the instrument to the dealer or
to the maker if need for dismantlin arises.
-9-

(6) Replacin lamp:
Before attemptin replacement,
turn the switch off(O) and take off the both plu s,
and remove the head.
Lay the microscope backward over on the stand arm.
You will find a small clampin knob on the bottom
plate and screw out with a coin or minus screwdriver.
A lamp in the mount will come out.
Hadle a new lamp with the plastic cover to avoid stains of the lamp with some
fin er rease, never touch a new lamp with naked fin ers. After replacement,
remove the plastic cover, shut the door, and then, screw in the knob firmly.
(
7
) Replacin
fuse
:
This microscope has 2 fuses (Live & Neutral).
After connectin the electric power cord,
if you could not et the
l
i ht when you switch on,
please check those 2 fuses.
Before attemptin replacement,
turn the switch off(O) and take off the both plu s,
t
urn the fuse holder to the arrow direction
with minus screwdriver, and you will be able
to take out a fuse in the holder.
Fuse
Please check the wire in the fuse. If the wire
was cut, it is needed to replace it to new one.
Before replacement, research the defect and release or repair it.
After replacement, turn it to the opposite direction
firmly.
Direct focus to CCD camera
Direct focus to CCD cameraDirect focus to CCD camera
Direct focus to CCD camera
Trinocular model provides the C-Mount tube with
the vertical tube as a standard accessory.
With this accessory, the direct ima e focused
by the objective can be obtained.
Compared with the secondary ima e produced by
usin TVA-45 camera adapter (see pa e 12),
the direct ima e becomes sharper and clearer,
while the field of view becomes narrower.
Pull out the slidin knob (ComponentsNo.17),
and detach the photo tube with the vertical tube
(Compornents No.12&14) from the trinocular
viewin head by looseni the lockin thumb screw
(CompornentsNo.15) beforehand.
Mount a CCD (or CMOS) camera onto the C-mount tube with the vertical tube
(ComponentsNo.28&14), and then, attach the vertical tube onto the viewin
head with the lockin screw (ComponentsNo.15). Connect the camera to the
monitor followin instructions provided with the camera and monitor.
If the ima e on the monitor is not parfocal with the ima e throu h eyepiece,
-10-