Nikon Elcipse TE2000 User manual

Turning on the system:
1) Turn on the main switch (A).
2) Turn on the computer (B).
3) If you need the mercury lamp,
turn on the switch (C) and then
press ignition (D).
4) Log onto the computer through
“MIC users” and start the
“NIS-Elements” software.
Turning off the system:
1) If you are the last user of the day, or if there is
more than 30 min interval before the next user is
booked, turn off the mercury lamp (C).
2) Close the software and “turn off” the computer
through the start menu.
3) Turn off the main switch (A).
4) Fill out the logbook.
Nikon TE2000 fluorescent microscope
A
B
C
D

Filter wheel with different
positions. Nr 6 is empty
and used for imaging DIC.
Shutter
for the
UV light
Light path control. If you are
looking at your sample (A),
taking fluorescence/DIC
images (B) or taking color
images (C).
A
B C
Control over the halogen light.
(The main switch on the halogen power
supply need to be on.) Regulate the
intensity here.
Switching between
objectives. Choose the right
objective for your sample.

Which camera do I choose?
Nikon DS-U1 (color) camera - or - MuTech MV 1500 (B/W) camera
Turn on main switch before use.

Adjusting the Köhler illumination
-this will increase image contrast-
A
B
C
C
1. Decrease the size of the diaphragm (A) so that you can see the
edges.
2. Bring the edges of the field iris onto focus by raising or lowering
the condensor focusing knob (B).
3. Senter the image of the field iris by using the condensor sentering
knobs (C).
4. Open the diaphragm so the edges lie just beyond the field of
view.
1.
2.
3.
4.
or

The difference between the objectives
HMC - Hoffman modulation contrast (objectives in position 5-6). The method converts phase
gradients into intensity variations to create 3D-like images, allowing observation in plastic dishes.
These objectives are suited for plastic dishes.
MC 30,55HMC LWD 40X
MC 20,40HMC LWD 20X
Ph23,7-2,70,60CFI Plan Fluor ELWD 40X
Ph10,40ELWD 20X
Ph 116,00,30CFI Plan Fluor 10X
17,10,13CFI Plan Fluor 4X
RemarksWD (mm)NADescription
6
5
4
3
2
1
Possit
ion
Correction collar. You have to adjust
the ring to the coverslip-petridish
thickness. Turn until the image looks
the sharpest.
Indicates the phase-ring. Use ”Ph2” for
this objective.
Indicates the NA. The higher the
number the better the resolution.

Correction collar. You have to adjust
the ring to the coverslip-petridish
thickness. Turn until the image looks
the sharpest.
Indicates the phase-ring. Use ”Ph2” for
this objective.
Indicates the NA. The higher the
number the better the resolution.
Collar not corrected Collar corrected
Use your fingers to turn the collar slightly. Then adjust
the focus. Now keep turning the collar until you get the
best sharp focus. This is very important for the quality
of your image.
Adjusting the correction collar on the objective

1) Start the NIS-Elements software.
2) If the stage/joystick is not responding,
make sure the stage is in “auto” position
and connect the stage through the
software.
“Devices” – “manage devices”, scroll
down to the “Märzhauser” and then click
“connect”.

1) If you want to acquire images
of multiple fluorochromes or
combine fluorescence and DIC
images – chose “capture
multichannel image” –
“multichannel setup”.
2) Tag off the settings you need and
click ok.
3) You are now ready to start
imaging. Click the multichannel
icon (left side) and remember to
go from “visual” to “camera
mode”.
Setting up a multichannel acquisition

1) Initiate stage and make sure
nothing is interfering (objectives
must be down) with the stage as it
will move to the outer limits.
2) Go to “acquire-large scan” and
define the image size you need.
Make sure you are located at the
upper left of your sample before
you start scanning.
3) Position the first frame in the top
left corner. 3 x 2 will give you a
large image as seen under.
Acquiring a large image with automated stage
1 2 3
6 5 4

Troubleshooting
•The stage/joystick is not responding.
The stage has not been initialized because it was on manual when
the software was opened. Make sure the stage is in “auto”
position and connect the stage through the software.
“Devices” – “manage devices”, scroll down to the “Märzhauser” and
then click “connect”.

Troubleshooting
•The camera setting is missing.
The previous user has probably closed the window.
Go to ”view – controls – camera settings”.

Troubleshooting
•I can’t find REX in order to save my data.
You need to connect to the server. When choosing REX, you will be
asked for a password. The password is ”************”.