Christ RVC 2-33 CDplus User manual

Translation of the original operating manual
sb
Rev. 3.3 of 03/07/2016
RVC 2-33 CDplus; ;with infrared heating
Version 08/2009
RVC 2-33 CDplus
with infrared heating
part no. 100244
Rotational Vacuum
Concentrator
Operating Manual
Please retain for later use!

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In case of inquiries, please state the following numbers:
Order number:
Serial number:
© Copyright by
Martin Christ Gefriertrocknungsanlagen GmbH
An der Unteren Söse 50
37520 Osterode am Harz
Germany
Tel.: [phone] / 5007-0
Fax: [phone] / 5007-12
Web: www.martinchrist.de
E-mail: [e-mail]

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Table of contents
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1General information.............................................................................................................9
1.1 Importance of the operating manual..................................................................................9
1.2 Intended use.....................................................................................................................9
1.3 Warranty and liability.........................................................................................................9
1.4 Copyright........................................................................................................................10
1.5 Explanation of symbols...................................................................................................10
1.6 Standards and regulations..............................................................................................10
1.7 Scope of supply..............................................................................................................10
2Layout and mode of operation.......................................................................................... 11
2.1 Layout of the RVC ..........................................................................................................11
2.1.1 Functional and operating elements............................................................................11
2.1.2 Name plate................................................................................................................12
2.2 Mode of operation...........................................................................................................13
2.2.1 Principle of the rotational vacuum concentration........................................................13
2.2.1.1 Advantages of the rotational vacuum concentration...........................................14
2.2.1.2 Examples of use ................................................................................................15
3Safety..................................................................................................................................16
3.1 Marking of the unit..........................................................................................................16
3.2 Explanation of the symbols and notes.............................................................................17
3.3 Responsibility of the operator..........................................................................................18
3.4 Operating personnel .......................................................................................................18
3.5 Informal safety instructions .............................................................................................18
3.6 Safety instructions ..........................................................................................................19
3.6.1 Electrical safety .........................................................................................................19
3.6.2 Mechanical safety......................................................................................................19
3.6.3 Fire prevention...........................................................................................................20
3.6.4 Thermal safety...........................................................................................................20
3.6.5 Chemical and biological safety ..................................................................................20
3.6.6 Safety instructions for evaporation.............................................................................21
3.7 Safety devices ................................................................................................................21
3.7.1 Lid lock device...........................................................................................................21
3.7.2 System check............................................................................................................21
3.7.3 Power failure safety...................................................................................................21
3.7.4 Earth conductor check...............................................................................................21
3.8 Procedures in the event of hazards and accidents..........................................................22
3.9 Remaining hazards.........................................................................................................22
4Storage and transport ....................................................................................................... 23
4.1 Storage conditions..........................................................................................................23
4.2 Dimensions and weight...................................................................................................23
4.3 Packaging.......................................................................................................................23
4.4 Transport safety device...................................................................................................24
4.5 On-site transport.............................................................................................................24

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5Set-up and connection ......................................................................................................25
5.1 Installation site................................................................................................................25
5.2 Power supply..................................................................................................................25
5.2.1 Connection ................................................................................................................25
5.2.2 Customer-provided fuses...........................................................................................25
5.3 Aeration and micro injection valve (Valve block).............................................................26
5.4 Vacuum connections.......................................................................................................27
5.5 Option: Pressure control valve and vacuum sensor........................................................28
5.6 Connection of a vacuum pump and/or a cooling trap ......................................................29
5.6.1 Withdrawal of the vapours by a vacuum pump...........................................................29
5.6.2 Condensation of the vapours upstream of the vacuum pump in a cooling trap...........30
5.6.2.1 Option: Remote control of the cooling trap via RVC ...........................................31
6Operation............................................................................................................................ 32
6.1 Initial start-up..................................................................................................................32
6.2 Switching the RVC on.....................................................................................................32
6.3 Opening and closing the lid.............................................................................................32
6.4 Installation of rotors and accessories..............................................................................33
6.4.1 Installation of angle rotors..........................................................................................33
6.4.2 Installation of swing-out rotors...................................................................................34
6.4.3 Installation of accessories..........................................................................................34
6.4.3.1 Tubes.................................................................................................................35
6.4.3.2 Rotor blocks.......................................................................................................36
6.4.3.3 Buckets..............................................................................................................36
6.5 CDplus control system....................................................................................................38
6.5.1 User interface............................................................................................................38
6.5.2 Mode .........................................................................................................................41
6.5.3 Main menu.................................................................................................................43
6.5.3.1 Changing the set values for the manual mode ...................................................43
6.5.3.2 Program administration......................................................................................45
6.5.3.3 Special functions................................................................................................48
6.5.3.4 Process and equipment information...................................................................49
6.5.3.5 Options..............................................................................................................50
7Malfunctions and error correction....................................................................................53
7.1 General malfunctions......................................................................................................53
7.1.1 Error messages.........................................................................................................53
7.1.2 Process messages ....................................................................................................54
7.1.3 System messages .....................................................................................................55
7.1.4 Emergency lid release...............................................................................................55
7.1.5 Small flange connections...........................................................................................57
7.2 Service contact...............................................................................................................58

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8Maintenance and service................................................................................................... 59
8.1 Maintenance...................................................................................................................59
8.1.1 Rotational vacuum concentrator ................................................................................59
8.1.2 Rotor chamber...........................................................................................................60
8.1.3 Accessories...............................................................................................................64
8.1.3.1 Aluminium accessories ......................................................................................65
8.1.4 Glass breakage .........................................................................................................65
8.2 Disinfection of the rotor chamber and accessories..........................................................66
8.3 Autoclaving.....................................................................................................................66
8.4 Service ...........................................................................................................................67
8.5 Return of defective parts.................................................................................................67
9Disposal.............................................................................................................................. 69
9.1 Disposal of the RVC........................................................................................................69
9.2 Disposal of the packaging...............................................................................................69
10 Technical data.................................................................................................................... 70
10.1 Ambient conditions .........................................................................................................71
10.2 Technical documentation................................................................................................71
11 Appendix ............................................................................................................................ 72
11.1 Mathematical relations....................................................................................................72
11.2 Rotor program.................................................................................................................73
11.3 Overview of evaporation times........................................................................................75
11.4 Brief operating instructions .............................................................................................77
11.5 EC declaration of conformity...........................................................................................79
11.6 Declaration of conformity –China RoHS 2......................................................................81
12 Glossary............................................................................................................................. 83
13 Index................................................................................................................................... 85

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1 General information
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
1 General information

1.1 Importance of the operating manual
A fundamental requirement for the safe and trouble-free operation of the
unit is to be familiar with the fundamental safety instructions and all
possible hazards.
The operating manual includes important information concerning the safe
operation of the rotational vacuum concentrator (RVC).
This operating manual, and in particular the notes on safety and hazards,
must be observed by all persons operating the unit.
In addition, the local rules and regulations for the prevention of accidents
must be complied with.

1.2 Intended use
CHRIST rotational vacuum concentrators are solely intended for
evaporation under rotation, e.g. for the following tasks:
•concentration of DNA/RNA, proteins etc.
•preparation of samples for HPLC/thin layer chromatography, gas
chromatography, mass-spectronomy
•isolation and synthesis of organic substances
•high-throughput-screening (HTS)
•general in-lab evaporation.
Any other use beyond this area of application is regarded as improper use.
Martin Christ Gefriertrocknungsanlagen GmbH cannot be held liable for any
damage resulting from such improper use.
The intended use also includes:
•observation of all the notes and instructions included in the operating
manual
•compliance with the inspection and maintenance instructions
•prohibition of any type of extensions to, or conversions of, the unit.

1.3 Warranty and liability
The warranty and liability are subject to our "General Terms and
Conditions" that were distributed to the operator upon the conclusion of the
contract.
Warranty and liability claims are excluded if they are due to one or several
of the following reasons:
•improper use
•non-compliance with the safety instructions and hazard warnings in the
operating manual
•improper installation, start-up, operation, and maintenance of the RVC.

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1.4 Copyright The copyright concerning the operating manual remains with Martin Christ
Gefriertrocknungsanlagen GmbH.
The operating manual is solely intended for the operator and their
personnel. It includes instructions and information that may not be
•duplicated,
•distributed, or
•communicated in any other way neither in full nor in parts.
Non-compliance may be prosecuted under criminal law.

1.5 Explanation of symbols
In this operating manual, the specialist terms that are explained in the
glossary (see chapter 12 - "Glossary") are marked by an arrow and printed
in italics (e.g. safety pressure).

1.6 Standards and regulations
EC-Declaration of conformity (see appendix)

1.7 Scope of supply
The scope of supply comprises:
•1 hexagon socket wrench, size 5
•1 L-key Torx®TX15
•1 screwdriver Torx®TX8
•1 operating manual
Accessories and commissioning
According to your order, our order confirmation, and our delivery note.

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2 Layout and mode of operation

2.1 Layout of the RVC

2.1.1 Functional and operating elements
1 Lid
2 Rotor chamber
3 Control panel (see chapter
6.5.1 - "User interface")
4 Lid lock device
5 Rotor shaft
6 Mains power switch
7 Electrical vacuum sensor
connection
8 Option: Serial Interface RS
232
9 Option: Serial Interface
Remote
10 Name plate (see chapter
2.1.2 - "Name plate")
11 Power supply connection
of the vacuum pump
12 Power supply connection
of the pressure control
valve
13 Valve block (see chapter
5.3 - "Aeration and micro
injection valve (Valve
block)")
14 Vacuum connection
15 Equipotential bonding
screw
16 Mains connection and
mains fuse protection
17 Power supply connection
of the stop valve
Fig. 1: Total view of the RVC
Fig. 1: Rear view of the RVC

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2.1.2 Name plate
1Serial number
2Type
3Nominal voltage
4Year of manufacture
(month/year)
5Part number
6Rated current /
apparent power
Fig. 2: Example of a name plate

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
2.2 Mode of operation

2.2.1 Principle of the rotational vacuum concentration
This method is used for the particularly quick and gentle evaporation,
drying, purification, and concentration of samples.
Rotational vacuum concentration is an alternative to rotary evaporators that
use high temperatures and operate nearly at normal pressure. Because of
the vacuum, the sample boils at low temperatures, and water as well as
organic solvents can be evaporated in a particularly gentle manner. Due to
the fact that the sample rotates like in a centrifuge, the Boiling retardation
is prevented so efficiently that low pressure can be used. As a result,
temperature-sensitive samples can be concentrated by evaporation without
being damaged.
The solvent can be collected in a cooling trap and disposed of immediately
if desired.
Fig. 3: Vapour pressure curve for ice and water

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Fig. 4: Vapour pressure curves of commonly used organic solvents

2.2.1.1 Advantages of the rotational vacuum concentration
•No foaming of the samples, minimum loss.
•Several samples can be dried simultaneously.
•Concentration of the sample on the bottom of the vessel. This is
particularly advantageous for small volumes or dilute solutions.
•Suitable for drying aqueous and solvent-containing samples.
•For volumes < 1 ml up to > 3 l.
•Reproducible drying processes thanks to controlled process
parameters, such as the rotor chamber temperature (energy input for
the evaporation) and vacuum (up to the automatic adjustment of the
optimum operating pressure –depending on the pump systems).
•Easy and safe solvent recovery.

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2.2.1.2 Examples of use
•DNA/RNA (mainly water, ethanol, or methanol as solvent)
•Oligo-synthesis, peptides
•PCR (polymer chain reaction)
•HPLC (mainly water/acetonitrile as solvent)
•Isolation/synthesis of organic substances
•Storage and handling of substances (substance libraries)
•Combinational chemistry
•High-throughput-screening (HTS)
•Analysis of food and environmental samples, toxicology
•Forensic applications
•General laboratory evaporation

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3 Safety

3.1 Marking of the unit
The following symbols are used for CHRIST RVC:
Dangerous voltage
I
On (Power)
Hot surface
0
Off (Power)
Caution! Risk of bruising
Name plate (see chapter
2.1.2 - "Name plate")
Attention, consult the
operating manual
CE mark in compliance
with the directive
2006/42/EC
Protective earth (ground)
Unplug the mains plug
Earth (ground)
China RoHS 2 mark
(only for China)
Arrow indicating the
direction of rotation
NOTE
Safety indications on the rotational vacuum concentrator must be kept
readable at all times. If necessary, they must be replaced.
NOTE
Not all of the symbols/labels are used for this RVC type.

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3.2 Explanation of the symbols and notes
This operating manual uses the following names and symbols to indicate
hazards:
DANGER
This symbol stands for a direct hazard to the life and health of persons.
Non-observance of these symbols causes serious health problems up to
life-endangering injuries.
DANGER
This symbol stands for a direct hazard to the life and health of persons
due to electrical voltage.
Non-observance of these symbols causes serious health problems up to
life-endangering injuries.
WARNING
This symbol stands for a potential hazard to the life and health of persons.
Non-observance of these symbols can cause serious health problems up
to life-endangering injuries.
CAUTION
This symbol indicates a potentially hazardous situation
Non-observance of these notes can cause minor injuries or damage to
property.
NOTE
This symbol indicates important information.

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3.3 Responsibility of the operator
The operator is responsible for authorising only qualified personnel to work
on the RVC (see chapter 3.4 - "Operating personnel").
The areas of responsibility of the personnel concerning the operation,
maintenance, and care of the unit must be clearly defined.
The safety-conscious work of the personnel in compliance with the
operating manual and the relevant EC and national health and safety
regulations as well as with the accident prevention regulations must be
checked at regular intervals (e.g. every month).
The RVC must be maintained regularly (see chapter 8 - "Maintenance and
service").

3.4 Operating personnel
Persons operating the unit must
•be familiar with the fundamental regulations concerning workplace
safety and accident prevention
•have read and understood this operating manual (and in particular the
safety sections and warning notes) and confirmed this with their
signature.

3.5 Informal safety instructions
This operating manual is part of the product.
•The operating manual must be kept at the location of use of the RVC.
Ensure that it is accessible at all times.
•The operating manual must be handed over to any subsequent owner
or operator of the RVC.
•Any changes made must be added to the operating manual.
•In addition to the operating manual, the general and local rules and
regulations concerning the prevention of accidents and the protection of
the environment must also be supplied.
•Safety and danger indications on the RVC must be kept readable at all
times. If necessary, they must be replaced.

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
3.6 Safety instructions

3.6.1 Electrical safety
CHRIST rotational vacuum concentrators are units of safety class I. The
RVCs are equipped with a three-wire power cord and a 230 VAC safety
plug with earthing contact. Please comply with the following points in order
to preserve this safety feature:
DANGER
•Ensure that the local mains voltage matches the nominal voltage that is
stated on the name plate.
•Do not place any dangerous material, e.g. glass vessels containing
liquid substances, within the safety area of 30 cm around the RVC.
Spilled liquids may get into the RVC and damage the electrical or
mechanical components.
•Work on the power supply system must only be performed by certified
electricians.
•Inspect the electrical equipment of the unit regularly. Defects such as
loose or burnt cables must be eliminated immediately.

3.6.2 Mechanical safety
In order to ensure the safe operation of the RVC, please comply with the
following points:
WARNING
•Do not use the RVC if it was installed incorrectly.
•Do not use the RVC without panels.
•Do not use the RVC with accessories that show signs of damage.
•Only use the RVC with accessories that have been approved by the
manufacturer. In case of doubt, contact the manufacturer (see chapter
7.2 - "Service contact").
•Do not hold your fingers between the lid and the housing when closing
the lid. Risk of crushing!
•Do not hit or move the RVC during its operation.
•Do not lean against or rest on the RVC during its operation.
•Check the RVC and accessories before every start-up for any visible
signs of damage.
•Do not evaporate any substances that could damage the material of
the rotors, rotor chamber or lid of the RVC in any way. Highly corrosive
substances (e.g. hydrogen chloride HCl) damage the material and
affect the mechanical strength of the rotors and accessories.
•Stop the RVC immediately in the event of a malfunction. Eliminate the
malfunction or contact the after-sales service of Martin Christ
Gefriertrocknungsanlagen GmbH (see chapter 7 - "Malfunctions and
error correction").
•Ensure that all repairs are performed only by authorised and
specialised personnel (see chapter 7.2 - "Service contact").


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3.6.3 Fire prevention
Fuses protect certain electrical circuits within the RVC against over-current
conditions.
GEFAHR
•Always use fuses of the same type and rating.
•Do not evaporate explosive or inflammable substances.
•Do not use the RVC within hazardous locations where there is a risk of
explosion.

3.6.4 Thermal safety
During the evaporation process, the housing, lid and interior of the RVC
can reach surface temperatures of more than +50°C.
DANGER
•Open and close the lid at the heat-insulated handle!
•Ensure that no limbs come into contact with hot unit parts or
accessories. Danger of burns!
•Wear heat-resistant gloves when inserting or removing the rotors.

3.6.5 Chemical and biological safety
If pathogenic, toxic, or radioactive samples are intended to be evaporated,
it is in the responsibility of the user to ensure that all necessary safety
regulations, guidelines, precautions, and practices are adhered to
accordingly.
DANGER
•Infectious, toxic, pathogenic, and radioactive substances must be
evaporated in suitable accessories. Take suitable precautions for your
own safety!
•Do not evaporate corrosive products (especially substances containing
acids) without special protective measures and devices such as, for
example, a cooling trap for the protection of the vacuum pump. Contact
the manufacturer (see chapter 7.2 - "Service contact").
•Special caution is necessary when handling azides, as a dangerous
explosive develops in combination with copper or nonferrous metals! It
is absolutely necessary to contact the manufacturer (see chapter 7.2 -
"Service contact").
•Keep informed about local measures to avoid harmful emissions
(depending on the substances to be evaporated).
•As personal protective equipment, safety gloves are required for the
use of the RVC.
The materials to be evaporated may, however, require additional
special safety measures (e.g. drying of infectious, toxic, radioactive, or
pathogenic substances)
