Christ RVC 2-33 CDplus User manual

Translation of the original operating manual
sb-bs
Rev. 3.6 of 22/11/2021
RVC 2-33 CDplus; ;with infrared heating
Version 08/2009
RVC 2-33 CDplus
with infrared heating
part no. 100244
Rotational Vacuum
Concentrator
Operating Manual
Please retain for later use!

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In case of inquiries, please state the following numbers:
Order number:
Serial number:
© Copyright by
Martin Christ Gefriertrocknungsanlagen GmbH
An der Unteren Söse 50
37520 Osterode am Harz
Germany
Tel.: [phone] / 5007-0
Fax: [phone] / 5007-12
Web: www.martinchrist.de
E-mail: [e-mail]

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Table of contents
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1General information.............................................................................................................9
1.1 Importance of the operating manual..................................................................................9
1.2 Intended use.....................................................................................................................9
1.3 Warranty and liability.......................................................................................................11
1.4 Copyright........................................................................................................................12
1.5 Explanation of symbols...................................................................................................12
1.6 Standards and regulations..............................................................................................12
1.7 Scope of supply..............................................................................................................12
2Layout and mode of operation..........................................................................................13
2.1 Layout of the RVC ..........................................................................................................13
2.1.1 Functional and operating elements............................................................................13
2.1.2 Name plate................................................................................................................14
2.2 Mode of operation...........................................................................................................15
2.2.1 Principle of the rotational vacuum concentration........................................................15
2.2.1.1 Advantages of the rotational vacuum concentration...........................................16
2.2.1.2 Examples of use ................................................................................................16
3Safety..................................................................................................................................17
3.1 Marking of the unit..........................................................................................................17
3.2 Explanation of the symbols and notes.............................................................................18
3.3 Responsibility of the operator..........................................................................................19
3.4 Requirements concerning the personnel.........................................................................20
3.5 Informal safety instructions .............................................................................................21
3.6 Safety notes concerning the transport, set-up and connection and initial start-up of the
freeze-dryer....................................................................................................................22
3.6.1 General hazards........................................................................................................22
3.6.2 Hazards caused by improper transport......................................................................22
3.6.3 Hazards caused by improper set-up..........................................................................22
3.6.4 Hazards caused by an improper connection..............................................................23
3.7 Safety notes concerning the operation............................................................................23
3.7.1 Electrical safety .........................................................................................................24
3.7.2 Hazards through the lid..............................................................................................24
3.7.3 Hazards due to damaged accessories.......................................................................24
3.7.4 Hazards due to hot surfaces......................................................................................25
3.7.5 Hazards due to flammable and explosive substances................................................25
3.7.6 Hazards caused by harmful products.........................................................................25
3.7.7 Hazards caused by contaminated condensate (defrosting water)..............................26
3.7.8 Safety instructions for evaporation.............................................................................26
3.7.9 Hazards due to the evaporation of harmful precursors...............................................27
3.8 Safety devices ................................................................................................................28
3.8.1 System check............................................................................................................28
3.8.2 Power failure safety...................................................................................................28
3.8.3 Lid lock device...........................................................................................................28
3.8.4 Earth conductor check...............................................................................................28

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3.9 Procedures in the event of hazards and accidents..........................................................29
3.10 Maintenance and cleaning of the rotational vacuum concentrator...................................30
3.11 Measures to ensure the safe operation of the rotational vacuum concentrator................31
3.12 Remaining hazards.........................................................................................................32
4Storage and transport ....................................................................................................... 33
4.1 Storage conditions..........................................................................................................33
4.2 Dimensions and weight...................................................................................................33
4.3 Packaging.......................................................................................................................33
4.4 Transport safety device...................................................................................................34
4.5 On-site transport.............................................................................................................34
5Set-up and connection ...................................................................................................... 35
5.1 Installation site................................................................................................................35
5.2 Power supply..................................................................................................................35
5.2.1 Type of connection ....................................................................................................35
5.2.2 Customer-provided fuses...........................................................................................35
5.3 Aeration and air injection valve (valve block) ..................................................................36
5.4 Vacuum connections.......................................................................................................37
5.5 Option: Pressure control valve and vacuum sensor ........................................................38
5.6 Connection of a vacuum pump and/or a cooling trap......................................................39
5.6.1 Withdrawal of the vapours by a vacuum pump...........................................................39
5.6.2 Condensation of the vapours upstream of the vacuum pump in a cooling trap...........40
5.6.2.1 Option: Remote control of the FD cooling trap via RVC......................................41
6Operation............................................................................................................................ 42
6.1 Initial start-up..................................................................................................................42
6.2 Switching the RVC on.....................................................................................................42
6.3 Opening and closing the lid.............................................................................................42
6.4 Installation of rotors and accessories..............................................................................43
6.4.1 Installation of angle rotors..........................................................................................43
6.4.2 Installation of swing-out rotors ...................................................................................44
6.4.3 Installation of accessories..........................................................................................44
6.4.3.1 Tubes.................................................................................................................45
6.4.3.2 Rotor blocks.......................................................................................................46
6.4.3.3 Buckets..............................................................................................................46
6.5 CDplus control system....................................................................................................48
6.5.1 User interface............................................................................................................48
6.5.2 Mode .........................................................................................................................51
6.5.3 Main menu.................................................................................................................53
6.5.3.1 Changing the set values for the manual mode...................................................53
6.5.3.2 Program administration......................................................................................55
6.5.3.3 Special functions................................................................................................58
6.5.3.4 Process and equipment information...................................................................59
6.5.3.5 Options..............................................................................................................60

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7Malfunctions and error correction....................................................................................63
7.1 General malfunctions......................................................................................................63
7.1.1 Error messages.........................................................................................................63
7.1.2 Process messages ....................................................................................................64
7.1.3 System messages .....................................................................................................65
7.1.4 Emergency lid release system...................................................................................65
7.1.5 Small flange connections...........................................................................................72
7.2 Service contact...............................................................................................................73
8Maintenance and service................................................................................................... 74
8.1 Maintenance...................................................................................................................74
8.1.1 General......................................................................................................................74
8.1.2 Rotor chamber...........................................................................................................76
8.1.3 Accessories...............................................................................................................80
8.1.3.1 Aluminium accessories ......................................................................................80
8.1.4 Glass breakage .........................................................................................................81
8.2 Disinfection of the rotor chamber and accessories..........................................................81
8.3 Autoclaving.....................................................................................................................82
8.4 Service ...........................................................................................................................82
8.5 Return of defective parts.................................................................................................83
9Disposal.............................................................................................................................. 85
9.1 Disposal of the RVC........................................................................................................85
9.2 Disposal of the packaging...............................................................................................85
10 Technical data.................................................................................................................... 86
10.1 Ambient conditions .........................................................................................................87
10.2 Technical documentation................................................................................................87
11 Appendix............................................................................................................................88
11.1 Mathematical relations....................................................................................................88
11.2 Rotor program.................................................................................................................89
11.3 Overview of evaporation times (RVC 2-33 CDplus with infrared heating)........................90
11.4 Brief operating instructions .............................................................................................92
11.5 EC declaration of conformity...........................................................................................95
11.6 Declaration of conformity –China RoHS 2......................................................................97
12 Glossary.............................................................................................................................99
13 Index................................................................................................................................. 101

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1 General information
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1 General information
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1.1 Importance of the operating manual
A fundamental requirement for the safe and trouble-free operation of the
unit is to be familiar with the fundamental safety instructions and all
possible hazards.
The operating manual includes important information concerning the safe
operation of the rotational vacuum concentrator (RVC).
This operating manual, and in particular the notes on safety and hazards,
must be observed by all persons operating the unit.
In addition, the local rules and regulations for the prevention of accidents
must be complied with.
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1.2 Intended use
CHRIST rotational vacuum concentrators are solely intended for
evaporation under rotation and vacuum. Possible areas of application
include:
•Concentration of DNA/RNA, proteins, etc.
•Sample preparation for HPLC/thin-layer chromatography, gas
chromatography and mass spectroscopy
•Isolation/synthesis of natural substances
•High-throughput screening (HTS)
•General evaporation tasks in laboratories
Any other use beyond this area of application is regarded as improper use.
Martin Christ Gefriertrocknungsanlagen GmbH cannot be held liable for any
damage resulting from such improper use.
Evaporation of solvent-containing products
(non-aqueous media)
In terms of the corrosion resistance, the use of organic solvents in aqueous
solutions is acceptable.
By design, rotational vacuum concentrators are chemically resistant against
most compounds that are typically used. However, by necessity, rotational
vacuum concentrators comprise several different materials, some of which
may be attacked and degraded by certain chemicals.
The manufacturing methods and/or conditions of exposure of the materials,
as well as the way the chemicals are applied, can influence the results.
Some of these factors are listed below:
•Manufacturing: Stress generated while sawing, sanding, machining,
drilling, polishing and/or forming.
•Exposure: Length of exposure, stresses induced during the service life
due to various loads (changes in temperature, etc.).
•Application of chemicals: by contact, rubbing, wiping, spraying, etc.

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With the exception of hydrochloric acid, nearly all standard solvents can be
used. For further details, see the table below.
Solvent
Stainless
steel
Aluminium
EPDM
PPS
Borosilicate
glass
Experience
Acetic acid 20%
+
+
o
+
+
?
Formic acid
o
o
-
o
+
?
Trifluoroacetic acid
(TFA)
+
+
+
+
+
?
Acetone
+
+
o
+
+
?
Acetonitrile
+
+
o
+
+
+
Cyclohexane
+
+
-
+
+
?
Dioxane
+
+
o
+
+
+
Dimethylformamide
+
+
o
+
+
?
Dimethyl sulfoxide
+
+
+
+
+
+
Dichloromethane
+
+
-
o
+
-
Ethanol
+
+
+
+
+
+
Isopropyl alcohol
+
+
+
+
+
?
Methanol
+
+
+
+
+
+
tert-Butanol
+
+
o
+
+
?
Toluene
+
+
-
o
+
+
Tetrahydrofuran
+
+
-
o
+
?
Pyridine
+
+
o
+
+
?
Legend:
+ No degradation to be expected
o Moderate degradation; limited use
- Severe degradation; infrequent use recommended;
immediate thorough cleaning required
Damage to the rotational vacuum concentrator and its accessories due to
chemicals can be significantly reduced by cleaning immediately after the
end of the programme. All parts of the rotational vacuum concentrator that
have come into contact with the product must be checked regularly for
signs of damage and replaced, if necessary.
NOTE
Solvents not included in the above table must not be used.

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Evaporation of solvent-containing products
Evaporation of acid-containing products (with the exception of the
substances listed under "Evaporation of solvent-containing products" with
the concentration levels mentioned therein) is only permissible if special
protective measures and equipment-related precautions are taken.
Otherwise, there is a risk of damage to property and personal injury.
Consultation of Martin Christ Gefriertrocknungsanlagen GmbH is absolutely
mandatory in order to define the measures that need to be taken!
The intended use also includes:
•Observation of all of the notes and instructions that are included in the
operating manual
•Compliance with the inspection and maintenance instructions
The following operations are regarded as NOT PERMISSIBLE:
•Use of the rotational vacuum concentrator if it is not properly installed.
•Use of the rotational vacuum concentrator if it is not in a perfect
technical state.
•Use of the rotational vacuum concentrator within hazardous locations
with an explosive atmosphere.
•Use of the rotational vacuum concentrator with unauthorised additions
or conversions without the written approval by Martin Christ
Gefriertrocknungsanlagen GmbH.
•Use of the rotational vacuum concentrator with accessories that have
not been approved by Martin Christ Gefriertrocknungsanlagen GmbH,
with the exception of commercially available vessels made of glass or
plastic.
•Evaporation of products that may react during the evaporation process
following the supply of high amounts of energy.
•Evaporation of azide-containing products.
•Evaporation of products that may damage the material of the chamber
walls, rotor/rotor hub, pipes or seals, or that may affect the mechanical
strength.
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1.3 Warranty and liability
The warranty and liability are subject to our "General Terms and
Conditions" that were distributed to the operator upon the conclusion of the
contract.
Warranty and liability claims are excluded if they are due to one or several
of the following reasons:
•improper use
•non-compliance with the safety instructions and hazard warnings in the
operating manual
•improper installation, start-up, operation, and maintenance of the RVC.
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1.4 Copyright The copyright concerning the operating manual remains with Martin Christ
Gefriertrocknungsanlagen GmbH.
The operating manual is solely intended for the operator and their
personnel. It includes instructions and information that may not be
•duplicated,
•distributed, or
•communicated in any other way neither in full nor in parts.
Non-compliance may be prosecuted under criminal law.
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1.5 Explanation of symbols
In this operating manual, the specialist terms that are explained in the
glossary (see chapter 12 - "Glossary") are marked by an arrow and printed
in italics (e.g. →safety pressure).
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1.6 Standards and regulations
EC-Declaration of conformity (see appendix)
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1.7 Scope of supply
The scope of supply comprises:
•1 hexagon socket wrench, size 5
•1 L-key Torx®TX15
•1 screwdriver Torx®TX8
•1 operating manual
Accessories and commissioning
According to your order, our order confirmation, and our delivery note.
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2 Layout and mode of operation
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2.1 Layout of the RVC
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2.1.1 Functional and operating elements
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1 Lid
2 Rotor chamber
3 Control panel (see
chapter 6.5.1 - "User
interface")
4 Lid lock device
5 Rotor shaft
6 Mains power switch
7 Electrical vacuum
sensor connection
8 Option: Serial Interface
RS 232
9 Option: Serial Interface
Remote
10 Valve block (see
chapter 5.3 - "Aeration
and air injection valve
(valve block)")
11 Power supply
connection of the
vacuum pump
12 Power supply
connection of the
pressure control valve
13 Name plate (see
chapter 2.1.2 - "Name
plate")
14 Vacuum connection
15 Equipotential bonding
screw
16 Mains connection and
mains fuse protection
17 Power supply
connection of the stop
valve
Fig. 1: Total view of the RVC
Fig. 2: Rear view of the RVC
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2.1.2 Name plate
1 Serial number
2 Type
3 Nominal voltage
4 Year of manufacture
(month/year)
5 Part number
6 Rated current /
apparent power
Fig. 3: Example of a name plate
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2.2 Mode of operation
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2.2.1 Principle of the rotational vacuum concentration
This method is used for the particularly quick and gentle evaporation,
drying, purification, and concentration of samples.
Rotational vacuum concentration is an alternative to rotary evaporators that
use high temperatures and operate nearly at normal pressure. Because of
the vacuum, the sample boils at low temperatures, and water as well as
organic solvents can be evaporated in a particularly gentle manner. Due to
the fact that the sample rotates like in a centrifuge, the →Boiling retardation
is prevented so efficiently that low pressure can be used. As a result,
temperature-sensitive samples can be concentrated by evaporation without
being damaged.
The solvent can be collected in a cooling trap and disposed of immediately
if desired.
Fig. 4: Vapour pressure curve for ice and water

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Fig. 5: Vapour pressure curves of commonly used organic solvents
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2.2.1.1 Advantages of the rotational vacuum concentration
•No foaming of the samples, minimum loss.
•Several samples can be dried simultaneously.
•Concentration of the sample on the bottom of the vessel. This is
particularly advantageous for small volumes or dilute solutions.
•Suitable for drying aqueous and solvent-containing samples.
•For volumes < 1 ml up to > 3 l.
•Reproducible drying processes thanks to controlled process
parameters, such as the rotor chamber temperature (energy input for
the evaporation) and vacuum (up to the automatic adjustment of the
optimum operating pressure –depending on the pump systems).
•Easy and safe solvent recovery.
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2.2.1.2 Examples of use
•DNA/RNA (mainly water, ethanol, or methanol as solvent)
•Oligo-synthesis, peptides
•PCR (polymer chain reaction)
•HPLC (mainly water/acetonitrile as solvent)
•Isolation/synthesis of organic substances
•Storage and handling of substances (substance libraries)
•Combinational chemistry
•High-throughput-screening (HTS)
•Analysis of food and environmental samples, toxicology
•Forensic applications
•General laboratory evaporation
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3 Safety
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3.1 Marking of the unit
The following symbols are used for Christ RVC:
Dangerous voltage
I
On (Power)
Hot surface
0
Off (Power)
Caution! Risk of bruising
Name plate (see chapter
2.1.2 - "Name plate")
Attention, consult the
operating manual
CE mark in compliance
with the directive
2006/42/EC
Protective earth (ground)
Unplug the mains plug
Earth (ground)
China RoHS 2 mark
(only for China)
Arrow indicating the
direction of rotation
NOTE
Safety indications on the rotational vacuum concentrator must be kept
readable at all times. If necessary, they must be replaced.
NOTE
Not all of the symbols/labels are used for this RVC type.
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3.2 Explanation of the symbols and notes
This operating manual uses the following names and symbols to indicate
hazards:
DANGER
This symbol stands for a direct hazard to the life and health of persons.
Non-observance of these symbols causes serious health problems up to
life-endangering injuries.
DANGER
This symbol stands for a direct hazard to the life and health of persons
due to electrical voltage.
Non-observance of these symbols causes serious health problems up to
life-endangering injuries.
WARNING
This symbol stands for a potential hazard to the life and health of
persons.
Non-observance of these symbols can cause serious health problems up
to life-endangering injuries.
CAUTION
This symbol indicates a potentially hazardous situation
Non-observance of these notes can cause minor injuries or damage to
property.
NOTE
This symbol indicates important information.
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Rotational Vacuum Concentrator RVC 2-33 CDplus
with infrared heating
3 Safety
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3.3 Responsibility of the operator
Operating personnel
The operator is obliged to ensure that the persons working on/with the
rotational vacuum concentrator
•has been specially commissioned by the operator for this purpose and
has received instruction on the hazards emanating from the device, the
output and end products as well as on the behaviour to be adopted in
the event of accidents and malfunctions and the measures to be taken
in such cases,
•are familiar with the fundamental health, safety and accident prevention
regulations,
•have been trained in terms of the operation of this system,
•have read and understood this operating manual (in particular the safety
sections and warning notes) and confirmed this with their signature.
–the rotor chamber up to the pressure control valve, aeration and
micro-injection valve block
–the glass lid
–the vacuum measurement technology.
The areas of responsibility of the personnel concerning the operation,
maintenance and care of the unit must be clearly defined.
The safety-conscious work of the personnel in compliance with the
operating manual and the relevant EC health and safety directives and the
national laws concerning health and safety and the prevention of accidents
must be checked at regular intervals (e.g. every month).
Working area
The operator must
•perform a risk assessment concerning potential accidents in connection
with the rotational vacuum concentrator and take design-related
countermeasures, if necessary.
•Perform a risk assessment in view of the specific hazards associated
with the processing of the respective product in the rotational vacuum
concentrator (e.g. ignition/explosion hazard, leakage of harmful product
residues at the outlets of the chamber) and take the corresponding
measures, if necessary.
•perform a compatibility test of all substances that are used in the
rotational vacuum concentrator (both products to be dried and cleaning
agents, etc.) that come into contact with chamber walls, pipes and
seals. The use of substances that damage the material (stainless steel
1.4404 and 1.4435, aluminium, borosilicate glass, EPDM and PPS) or
weaken the mechanical strength is prohibited.
•have the system maintained at regular intervals (see chapter 3.4 -
"Requirements concerning the personnel").
•Any parts or components that are not in perfect working order must be
replaced without delay.

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Additional points concerning the evaporation of solvent-containing products
With regards of corrosion resistance, the use of some organic solvents in
aqueous solutions is acceptable.
Under certain circumstances, the evaporation of products containing
solvents may lead to the formation of explosive mixtures. This is why the
operator must draw up special operating instructions/SOPs including
precise instructions
•concerning the chamber pressure and temperature for every product
that is to be processed in the RVC,
•concerning the inspection of the RVC in view of damage caused by the
solvent that is used (see chapter 1.2 - "Intended use", section
"Evaporation of solvent-containing products").
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3.4 Requirements concerning the personnel
DANGER
Risk of injury if the personnel are not sufficiently qualified
If unqualified personnel perform work on the freeze-dryer or are present in
the danger zone of the freeze-dryer, hazards result that can cause serious
injuries and considerable damage to property.
•Ensure that all the tasks are performed by personnel with the
corresponding qualifications.
•Ensure that unqualified personnel stay clear of the danger zones.
DANGER
Risk of fatal injury to unauthorised persons due to hazards in the
danger zone or work area
Unauthorised persons who do not fulfil the requirements described herein
are not aware of the hazards in the work area. This is why there is a risk
of serious or even fatal injuries for unauthorised persons.
•Ensure that unauthorised persons stay clear of the danger zone and
work area.
•If in doubt, address these persons and instruct them to leave the
danger zone and work area.
•Interrupt any running work if unauthorised persons are present in the
danger zone or work area.
This manual uses the following personnel qualifications for various areas of
activity:
Qualified electrician
Due to their special training, knowledge, experience and familiarity with the
relevant standards and regulations, qualified electricians are in the position
to perform work on electrical systems and to autonomously identify and
prevent possible hazards.
Qualified electricians have been specifically trained for the environment in
which they work and they are familiar with all the relevant standards and
regulations.
Qualified electricians must fulfil the requirements as set out in the
applicable legal provisions concerning the prevention of accidents.