Cleaver Scientific CSL-CELLAS User manual

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Cellulose Acetate
Tank
Instruction Manual
Catalogue Numbers
CSL-CELLAS CSL-CELLASMINI
Record the following for your records:
Model _____________________
Catalogue No. _____________________
Date of Delivery _____________________
Warranty Period _____________________
Serial No. _____________________
Invoice No. _____________________
Purchase Order No. _____________________

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Contents
Instruction Manual 1
Catalogue Numbers 1
Contents 2
Safety Information 3
Packing List 4
Operating Instructions 5
Usage Guidance and restrictions 5
Setting up the Cellulose Acetate Tanks 5
Fitting Electrode cables 5
Preparation and Gel running 6
Example of Staining 8
Example of Solutions 8
Care and Maintenance 8
Cleaning Cellulose Acetate Units 8
RNAse Decontamination 9
Warranty 10

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Safety Information
When used correctly, these units pose no health risk. However, these units
can deliver dangerous levels of electricity and are to be operated only by
qualified personnel following the guidelines laid out in this instruction manual.
Anyone intending to use this equipment should read the complete manual
thoroughly. The unit must never be used without the safety lid correctly in po-
sition. The unit should not be used if there is any sign of damage to the exter-
nal tank or lid.
These units comply with the following European directives:
2006/95/CE Low Voltage Directive and 2014/30/UE (official Title 2004/108/EC)
EMC Electromagnetic Compatibility
By virtue of the following harmonised standards:
BS EN IEC 61010-1: 2010 Safety Testing of Lab Equipment
BS EN IEC 61326-1:2013 EMC Electro Magnetic Compatibility

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Packing List
SKU
Main Unit and Lid
Connecters
Strip Holders
Instruction
Manual
CSL-CELLAS
CSL-CELLAS
CSL-CAB
CSL-STRPHO
CELLAS
CSL-CELLASMINI
CSL-CELLASMINI
CSL-CAB
CSL-STRPHOMINI
CELLAS
Packing List Checked by: ________________________
Date: ________________________
The packing lists should be referred to as soon as the units are received to
ensure that all components have been included. The unit should be checked
for damage when received.
Cleaver Scientific is liable for all missing or damaged parts / accessories within
7 days after customers have received this instrument package. Please contact
Cleaver Scientific immediately regarding this issue. If no response within such
period is received from the customer, Cleaver Scientific will no longer be liable
for replacement/damaged parts.
Please contact your supplier if there are any problems or missing items.

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Operating Instructions
Usage Guidance and restrictions
•Maximum altitude 2,000m.
•Temperature range between 4°C and 65°C.
•Maximum relative humidity 80% for temperatures up to 31OC decreas-
ing linearly to 50% relative humidity at 40OC.
•Not for outdoor Use.
This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.
POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution
occurs.
Occasionally, however, a temporary conductivity caused by condensation
must be expected”.
Setting up the Cellulose Acetate Tanks
Fitting Electrode cables
•Note the position of the lid on the unit. This shows the correct polarity
and the correct orientation of the cables, black is negative and red
positive.
•Remove the lid from the unit. Note if the lid is not removed, fitting the
cables may result in un-tightening of the gold plug and damage to the
electrode.
•Screw the cables into the tapped holes as fully as possible so that there
is no gap between the lid and the leading edge of the cable fitting.
•Refit the lid.
The unit is now ready to be used.

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Preparation and Gel running
The following protocol is a guideline only.
Please follow the manufacturers recommend instructions with the types of
Cellulose Acetate strip and samples being used.
1. Position the wick carrying bars in the tank such that the distance be-
tween them is just longer then the strip to be used when applied
lengthways.
2. Cut to the appropriate size and position the electrode wicks in the
tank so that they overhang the wick carrying bars with the base sit-
ting in the buffer tanks, see diagram below. Filter paper or paper
towels can be used as the wicks.
3. Equilibrate the strip in buffer with agitation for 10 –15 minutes. Please
see page 11 for details on commonly used buffers.
4. Fill the tank with 180ml of buffer per chamber side, 360ml in total for
the CSL-CELLAS unit and 100ml of buffer per chamber side, 200ml in
total for CSL-CELLASMINI
5. Holding the strip by the edges only in gloved hands, blot the strip in
between two pieces of filter paper.

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6. Place the strip on top of the wicks and between the bars with the
sample end at the negative electrode. Make sure there is good con-
tact by pushing down on the ends of the strip. Glass slides can be
placed over each end to further ensure a good contact.
7. Apply the samples at the negative end of the cellulose acetate
plate. Usually a small amount of dye can also be loaded to indicate
sample migration distance. The samples should be kept as cold as
possible before application to prevent denaturation.
8. Place the safety lid onto the tank and connect the leads to a power
supply.
9. Electrophorese at 200 volts for 15 –30 minutes or following cellulose
acetate strips set up.
10. Once electrophoresis is complete, the strip can be stained.

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Example of Staining
Ponceau S
1. Immerse the strips in about 50 ml of Ponceau S staining solution for 5 to
10 min. (Amidoblack may be used if preferred) with agitation.
2. Remove Ponceau
Example of Solutions
Running Buffer
•Tris Hippurate 0.05 M, pH 8.8
•Barbital Tris 0.05 M
•Tri Glycine pH9.5
Care and Maintenance
Cleaning Cellulose Acetate Units
Units are best cleaned using warm water and a mild detergent. Water at
temperatures above 60°C can cause damage to the unit and components.
The tank should be thoroughly rinsed with warm water or distilled water to
prevent build-up of salts, but care should be taken not to damage the en-
closed electrode and vigorous cleaning is not necessary or advised.
Air drying is preferably before use.
The units should only be cleaned with the following:
Warm water with a mild concentration of soap or other mild detergent.
Compatible detergents include dishwashing liquid, Hexane and Aliphatic hy-
drocarbons
The units should not be left to in detergents for more than 30 minutes.
The units should never come into contact with the following cleaning agents,
these will cause irreversible and accumulative damage:
Acetone, Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Iso-
propyl alcohol, Alkalis.

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RNAse Decontamination
This can be performed using the following protocol:
Clean the units with a mild detergent as described above.
Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.
Rinsed with 0.1% DEPC-(diethyl pyro carbonate) treated distilled water,
Caution: DEPC is a suspected carcinogen. Always take the necessary pre-
cautions when using.
RNaseZAP™ (Ambion) can also be used. Please consult the instructions for
use with acrylic gel tanks.

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Warranty
The Cleaver Scientific Ltd. (CSL) units have a warranty against manufacturing
and material faults of twelve months from date of customer receipt.
If any defects occur during this warranty period, CSL will repair or replace the
defective parts free of charge.
This warranty does not cover defects occurring by accident or misuse or de-
fects caused by improper operation.
Units where repair or modification has been performed by anyone other
than CSL or an appointed distributor or representative are no longer under
warranty from the time the unit was modified.
Units which have accessories or repaired parts not supplied by CSL or its asso-
ciated distributors have invalidated warranty.
CSL cannot repair or replace free of charge units where improper solutions or
chemicals have been used. For a list of these please see the Care and
Maintenance subsection.
If a problem does occur, then please contact your supplier or Cleaver Scien-
tific Ltd:
Cleaver Scientific Ltd.
Unit 41, Somers Road Industrial Estate
Rugby, Warwickshire, CV22 7DH
Tel: [phone]
Email: [e-mail]