CoolLED pE-100 Series User manual

DOC-012 Iss 7 1
User Manual
pE-100

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Table of Contents
1. Introduction.................................................................................................................. 3
2. Safety Precautions........................................................................................................ 4
3. Getting Started –System Components ........................................................................ 6
4. Installation and Setup................................................................................................... 7
5. Operation - Manual Control ......................................................................................... 9
6. Remote Operation –TTL............................................................................................. 10
7. Optical Setup .............................................................................................................. 12
8. Settings/Additional Information................................................................................. 13
9. Routine Care and Maintenance.................................................................................. 14
10. Fitting the pE-100 to a Different Microscope............................................................. 15
11. pE-100 Liquid Light Guide Option............................................................................... 16
12. Product Specifications ................................................................................................ 18
13. Product Options and Order Codes.............................................................................. 18
14. Warranty and Repairs................................................................................................. 19
15. Compliance and Environmental ................................................................................. 19
16. Contact Details............................................................................................................ 20

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1. Introduction
CoolLED’s pE-100 has been developed for applications requiring a single LED
excitation wavelength. It is perfect for clinical applications such as routine
screening (e.g. using Auramine for Tuberculosis), for research applications
requiring precise intensity control and fast-switching, or for
electrophysiology applications where light has to be delivered to a specific
location. The user can select from 20 different LED wavelengths, ranging
from the near-UV at 365 nm to the near-IR at 770 nm.
With a comprehensive range of microscope adaptors, the pE-100 can be
fitted to most current and older microscopes. The result is a safe, convenient
illumination system which will last for many years without any additional
operating costs.
This manual should give you all the information required to install and
operate your new illumination system.
Additional information can be found on our website at www.coolled.com

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2. Safety Precautions
While LEDs are a much safer illumination system than the mercury and
metal halide lamps that they replace in microscopy applications, precautions
should still be taken with this product.
When operating or maintaining this product, please observe the following
safety precautions at all times. Failure to do so may result in personal injury
or damage to other items.
Please ensure that only the power supply and cord supplied are used with
this equipment.
The AC cord supplied with this light source must only be used with the
equipment supplied.
2.1.
UV light may be emitted from this product depending on the
version/wavelength selected. Avoid eye and skin exposure. Never look
directly into the light output beam from the Light Source or accessories. The
emissions could damage the cornea and retina of the eye if the light is
observed directly.
2.2.
Always ensure that the Light Source is securely attached to the microscope
(either directly or with a light guide and collimator, depending on the
version) prior to turning on the power. This will minimise the risk of injury
and damage.
2.3.
If for any reason the Light Source is to be operated when not attached to a
microscope, all personnel should wear eye shielding and clothing to protect
the exposed skin.
2.4.
Disconnecting the mains supply is achieved by unplugging the power cord
from the power supply block or the Light Source. Only plug in the power
cable, once the Light Source is attached to the microscope.
2.5.
There are no serviceable parts within the Light Source. Removing any of the
screws and covers will result in the safety of the Light Source being impaired.
The DC power supply unit should be inspected periodically throughout the
lifetime of the system.

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2.6.
Any electronic equipment connected to this product must comply with the
requirements of EN/IEC 60950.
2.7.
To clean the exterior of the Light Source, use a slightly dampened cloth with
a simple water/detergent solution only. Avoid the optical surfaces and
lenses. Cleaning of optics should only be carried out using optical wipes and
fluids. Please note that the DC power supply unit should be isolated prior to
cleaning.
2.8.
This product conforms to the requirements of the Safety Standards as
follows:
EN/IEC 61010-1:2010 Safety Requirements for Electrical Equipment
for Measurement, Control and Laboratory use.
EN62471:2008 Photo-biological Safety of Lamps and Lamp
Systems/Guidance on manufacturing
requirements relating to non-laser optical
radiation safety. Risk Group 3.
2.9. EMC compliance
This product is tested to the requirements of standard IEC/EN 61326-1
concerning electromagnetic compatibility. This is a Class A product. In a
domestic environment this product may cause radio interference in which
case the user may be required to take adequate measures.
All warnings may not be applicable depending on the version/wavelength being used.

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3. Getting Started –System Components
The CoolLED pE-100 Illumination System is supplied with the following
components:
1. LED Light Source.
2. Manual Control Pod.
3. Microscope adaptor for specific microscope model (Direct attachment
version only).
4. Liquid light guide (light guide version only).
5. DC Power Supply Type GST25A12-P1J.
6. IEC Power Cable (not shown).
7. User Guide (not shown).
If any components are missing or appear damaged, please contact CoolLED
immediately.

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4. Installation and Setup
4.1.
Carefully unpack the components from the shipping cartons.
4.2.
Insert the Control Pod cable into the LED Light Source.
4.3.
Connect the power connector from the DC power supply to the Control Pod
as shown. Ensure that the DC power supply is the one supplied with the
product. Using non CoolLED power supplies may damage the Light Source
and will invalidate the warranty. At this stage do not connect the mains
power lead to the DC power supply.

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4.4.
Attach the LED Light Source to the epi-fluorescence port on your
microscope. Your pE-100 will have been supplied with a compatible fitting
for the microscope you specified at order. Attach the Light Source ensuring
that it is secure and true/flush with the microscope.
4.5.
Ensure that there is free airflow around the LED Light Source so that the
cooling system is not impaired. A gap of 200 mm on either side is sufficient.
4.6.
With the LED Light Source now attached to the microscope it is safe to
connect the mains power. Connect the mains lead supplied to a convenient
socket, plug in the IEC connector into the DC power supply and switch the
power on at the socket.

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5. Operation - Manual Control
5.1.
Manual Control Pod Operation on/off.
The pE-100 is easily controlled from the manual Control Pod. LEDs are
switched on and off by pressing the ‘on/off’ button.
5.2.
At start-up the Light Source will revert to the same settings that were set
when it was last powered down. New Light Sources are supplied with the
settings as shown.
5.3.
Adjust the intensity of the Light Source by pressing the up and down
intensity buttons. This is displayed in 1 % steps between 20 % and 100 %.
Between 0 % and 20 % this figure is displayed in 0.2 % intensity steps.
State of light source
displayed here.
Either OFF or ON.
On/Off
button
Intensity control
buttons. ↑to
increase the light
output. ↓to decrease
the power output.
Light intensity
level. 0-100 %.

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6. Remote Operation –TTL
6.1.
The pE-100 can be controlled remotely via a TTL signal.
6.2.
TTL control uses the single BNC socket on the reverse of the Control Pod.
6.3.
The TTL signal controls the on/off function of the Light Source. A TTL ‘high’
will cause the LEDs to be on, independent of the state of the on/off button.
The intensity of the LEDs are manually set on the Control Pod.

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6.4.
The TTL input circuit has been designed to maximise the switching speed of
the LEDs to give the user precise control of the excitation light reaching the
sample.
6.5.
With fast repetitive switching, the Control Pod display will not be able to
respond at the same speed and so can get out of synch. If after a train of
pulsing, the display on the Control Pod indicates that the LEDs are on while
they are actually off, simply press the ‘on/off’ button to reset the display
correctly.
Note: Switching speeds vary slightly depending on LED wavelength and the intensity that is used.

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7. Optical Setup
7.1.
The pE-100 has been designed to work on the majority of fluorescence
microscopes, both new and old. As would be expected, there is some
variation in the optical path and elements within every microscope. In order
to accommodate these variations, the pE-100 is supplied with a small
adjustment which allows the user to optimise the performance of the
illumination system when it is first fitted. This is a one-time adjustment. No
further adjustment will be required during the life of the product unless
changes are made to the microscope or the illumination system is fitted to a
different microscope (See Section 10 for further information on fitting to a
different microscope).
7.2.
To make the adjustment, set up a typical sample on the microscope that
gives an image over the whole field of view. Loosen the thumbscrew and
slide the LED head back and forth until you achieve the maximum brightness
with an even field of view. Tighten the thumbscrew to prevent the setting
from changing.
Light source too far in.
Light source in optimal
position.
Light source too far out.
Thumb Screw
Move light source back
and forth to adjust.

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8. Settings/Additional Information
8.1.
Display Backlight and Contrast settings.
Upon switching on the Control Pod a
chequered pattern shall appear on the
display. If any button is pressed at this
stage, then you shall be taken through a
setup sequence.
The first option displayed is the contrast
adjustment. The contrast setting can be
adjusted using the two arrow buttons.
This is adjusted in 5 % increments.
After having set the contrast press the
On/Off button once to continue.
The next screen displayed shall be the
Backlight adjustment. The Backlight can
be adjusted using the two arrow buttons.
This is adjusted in 5 % increments.
After having set the backlight press the
On/Off button once to continue.

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8.2.
System Information
To display the system information,
complete the steps described in Section
8.1.
The first screen shown displays the
firmware version of the system.
Using the arrow buttons, information will
be displayed about the head code, the
head temperature, the sink temperature
and the sink voltage.
After having viewed the system information press the On/Off button once
to continue.
8.3.
LED usage information
The system automatically records the
total time that the LEDs are on.
To view this information complete
Sections 8.1 & 8.2. The system displays a
figure in hours.
Once the LED usage information has been
viewed press the On/Off button to
continue. This button press will return
you to the standard operation display.
9. Routine Care and Maintenance
9.1.
The pE-100 requires little or no maintenance throughout its life. There are
no field serviceable parts so there is no need to remove the covers.
9.2.
Cleaning of the external surfaces can be carried out with a mild soap and
water solution used to lightly dampen a lint-free cloth. Ensure that no liquid
is allowed to enter the product through vents and panel edges. Avoid
optical surfaces.

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9.3.
Cleaning of optical surfaces may be necessary if debris or finger prints
accidently come into contact with the lens during installation. In the first
instance remove any loose debris with an air duster (aerosol or rubber
blower).
9.4.
Fingerprints or other liquid type contaminants should be removed using
standard lens cleaning procedures. Do not flood the lens surfaces with fluid
as liquid could enter the product and cause damage.
10. Fitting the pE-100 to a Different Microscope
10.1.
The pE-100 has been designed to be compatible with a wide range of
fluorescence microscopes, both new and old. Every microscope manufacturer
has one or a number of methods of attaching the fluorescence light source.
CoolLED has designed a comprehensive range of adaptors to match these
microscopes.
10.2.
If you intend to use the pE-100 on a different microscope than the make
and/or model that it was originally intended for, please contact CoolLED to
check whether the Light Source will be compatible in its current form. We will
be able to advise whether an adaptor swap or optical adjustment is required.
Please note that even if a microscope accepts the same physical adaptor, the
internal optics may differ.

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11. pE-100 Liquid Light Guide Option
11.1.
The pE-100 is available with a 3 mm liquid light guide rather than using a
direct attachment adaptor. This enables the Illumination System to be used
on microscopes which just have a liquid light guide input, the collimating
optics being an integral part of the microscope.
11.2.
The liquid light guide is fixed to the Light Source by CoolLED. There is a
choice of 1 metre, 1.5 metre or 3 metre light guides that can be fitted. This is
to be specified at the time of purchase. The light guide is secured in place
and is not swappable by the user.
11.3.
Do not bend the liquid light guides through sharp corners. It is
recommended to ensure a minimum bend radius of 75 mm. Ensure that the
Light Source has a sufficient clearance to ensure adequate airflow for the
cooling system.

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11.4.
The use of a liquid light guide will be attractive for use in electrophysiology
as this allows the Light Source to be placed outside the Faraday cage to
reduce electrical noise close up to the samples. The pE-Universal Collimator
is available for these applications. See Section 14 for details and order codes.
11.5.
When using this collimator, it is important to set up the optics correctly to
optimise the performance of the Illumination System. Full setup instructions
are given in the separate user manual for the pE-Universal Collimator.

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12. Product Specifications
12.1.
Power requirements
100-240 V a.c 50/60 Hz 0.7 A
12.2.
Power consumption
Standby mode max 2.2 W
Full Intensity (100 % setting) max 25 W
12.3.
Dimensions:
Light Source (Direct fit) 60 mm (w) x 170 mm (d) x 70 mm (h)
-weight 0.38 kg
Light Source (Light guide version) 52 mm (w) x 95 mm (d) x 52 mm (h)
-weight 0.38 kg
Control Pod 102 mm (w) x 110 mm (d) x 50 mm (h)
- weight 0.55 kg
Power Supply 55 mm (w) x 95 mm (d) x 40 mm (h)
- weight 0.19 kg
12.4.
Environmental Operating Conditions
Operating 5 –35 °C
13. Product Options and Order Codes
See website (Microscope Illuminators | LED Illumination Systems | CoolLED)
for full details of product options and order codes.

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14. Warranty and Repairs
Please refer to CoolLED’s current Warranty Policy available on our website
https://www.coolled.com/support/coolled-warranty/. Although warranty
terms are fixed at the time of ordering according to the terms and conditions
of sale in place, the Warranty Policy may be subject to periodic change so
please check to avoid confusion.
For any warranty queries or in the event of the product developing a fault,
make contact with [email protected]m for further assistance. You shall be
asked to provide your microscope make and model, the product serial
number and a brief description of the issue. You shall then be issued with a
Support Case to manage your issue.
15. Compliance and Environmental
For current compliance statements and environmental information please
refer to our website https://www.coolled.com/support/environment/
15.1. CoolLED’s Recycling Program
At CoolLED, we recognize the importance of preserving the global
environment. We are proud to provide a Recycling Program that enables
CoolLED customers and end-users to send back used CoolLED Light Sources
for recycling, free of charge.
Together we can reduce the burden on our environment through
responsible disposal and recycling of End-of-Life Light Sources. You can help
us by filling in our online contact form and providing us with your contact
details and the serial number of the CoolLED Light Source that you wish to
return and we will collect it free of charge.
If you are taking delivery of a replacement CoolLED Light Source, why not
send the old one back in the packing box of the new one?

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16. Contact Details
CoolLED Ltd
26 Focus Way
Andover
Hants
SP10 5NY
UK
Phone [phone] (Worldwide)
1-800-877-0128 (USA + Canada)
Email [email protected]
Online www.coolled.com