CYBIO CyBi-Well 96 User manual

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
CyBi®-Well
your solutions
CyBio


User Manual
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Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

CyBi®-Well Release:09/2008 III

Registered trademarks:
CyBi®, CyBio®. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CyBio AG, Jena, Gemany
TitletoallothertrademarksorbrandswhicharereferencedinthisUserManualbelongs
totheirlegalowners.
Copyright ©Copyright2008CyBioAG.Allrightsreserved.Nopart
ofthisdocumentationmaybeduplicated,photocopied,
savedtoastoragesystemortransferredtoelectronic
mediawithoutpriorwrittenpermissionofthepublisher.
Publisher
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Tel:+49-3641/351-0
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Ordering number OL3391-25-001BLE05
Document type TranslationofOriginalinstructions
Serial number Refertoproductnameplate
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

IV Release:09/2008 CyBi®-Well

1
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

CyBi®-Well Release:09/2008 V

Table of contents
1 General Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 GeneralUserManualAdvice.....................................1
1.2 IntendedPurpose.............................................2
1.3 ConformingUse..............................................2
1.4 Warranty&Liability............................................3
1.5 ScopeofDelivery.............................................3
2 Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1 GeneralData.................................................5
3 Safety Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1 General.....................................................9
3.2 Standards&Guidelines.........................................9
3.3 NRTLCertification.............................................9
3.4 Symbols&SignalWordsUsedThroughoutThisManual...............10
3.5 SafetyLabellingProvidedattheSystem............................10
3.6 MechanicalHazards..........................................12
3.7 GeneralSafetyNotes..........................................14
3.8 RequirementsOnOperatingPersonnel............................14
3.9 SafetyRequirementsforTransportation&Installation.................15
3.10 SafetyNotesforOperation.....................................15
3.10.1 General.................................................15
3.10.2 ExplosionProofness,FirePrevention............................15
3.10.3 Electrical ................................................16
3.10.4 Maintenance&Care .......................................16
3.10.5 HandlingofDangerousSubstances ............................17
3.10.6 ChemicalResistance........................................17
3.11 RulesofConductinCaseofEmergency...........................18
4 Technical Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1 General....................................................19
4.1.1 SystemSetupWithOperationControlElements...................20
4.1.2 ProductVersions ..........................................22
4.1.3 CyBi®-WellPipettingUnit ...................................22
4.1.4 PipettingHead ............................................22
4.1.5 CyBi®-TipTray ............................................23
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VI Release:09/2008 CyBi®-Well

4.1.6 PipettingPiston...........................................23
4.1.7 LCDDisplay ..............................................23
4.1.8 3-,4-or5-PlaceCarriage(LinearTransportingSystem)..............24
4.1.9 RotaryTable(CircularTransportingSystem)......................24
4.1.10 DropProtector(CircularTransportingSystem) ....................24
4.1.11 LifterWithTable ..........................................24
4.1.12 Keyboard ................................................24
4.2 Function...................................................25
5 Transportation & Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1 Transportation..............................................27
5.2 Storage....................................................28
6 Initial Start-Up & Routine Start-Up Procedure . . . . . . . . . . . . . . . . . . 29
6.1 SiteRequirements............................................29
6.1.1 InstallationRequirements ....................................29
6.1.2 FootprintRequirements.....................................29
6.1.3 EnergySupplies...........................................30
6.2 Configuration&Start-Up......................................30
6.3 FunctionTests...............................................31
6.3.1 PrecisionTest .............................................31
6.3.2 AccuracyTest.............................................32
6.3.3 LeakTest ................................................33
7 Manual Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.1 OperatingModes............................................35
7.2 OperationControlElements....................................35
7.3 Start......................................................37
7.4 ManualOperationControl.....................................38
7.4.1 General.................................................38
7.4.2 SpecialFunctions ..........................................39
7.4.3 SystemConfiguration ......................................39
7.4.4 ManualMode ............................................42
7.5 SpecificPipettorFeatures......................................52
7.5.1 VolumeCycle.............................................54
7.5.2 WaitingTime .............................................54
7.5.3 PrecisionPerformance......................................54
7.5.4 FurtherUsefulOperatingAdvice ..............................55
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CyBi®-Well Release:09/2008 VII

8 Fault Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
9 Maintenance & Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
9.1 SafetyNotes................................................59
9.2 MaintenanceWork...........................................60
9.2.1 Overview ................................................60
9.2.2 Lubricatethetransportrails(lineartransportingsystem) .............61
9.2.3 CleanLifter ..............................................61
9.2.4 TipMagazine .............................................62
9.2.5 CleanCarriage(LinearTransportingSystem) .....................63
9.2.6 CleanRotaryTable.........................................63
9.2.7 SealingPlate .............................................64
10 Shutting Down. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
11 Accessories&Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
11.1 Pipettingtips................................................67
11.2 FurtherAccessories...........................................67
12 Waste Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
12.1 Reagents...................................................69
12.2 System&Accessories.........................................69
A 1 Declaration of Safeness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

VIII Release:09/2008 CyBi®-Well

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

CyBi®-Well Release:09/2008 IX

Table of illustrations
Fig. 1: Warningnotesandsafetydevicesatthesystem......................12
Fig. 2: Hazardousareaofpipettingunit..................................12
Fig. 3: Hazardousareaoftransportsystem ...............................13
Fig. 4: CyBi®-Wellwithlineartransportingsystem ..........................19
Fig. 5: CyBi®-Wellwithcirculartransportingsystem ........................20
Fig. 6: BasicCyBi®-WellSetup&Controls ................................20
Fig. 7: CyBi®-Wellrearsideviewwithterminals............................21
Fig. 8: PipettingheadandCyBi®-TipTray .................................23
Fig. 9: LCDdisplayandcontrolelements .................................35
Fig. 10: Menustructure...............................................38
Fig. 11: Aspiratewithadditionalstroke ...................................52
Fig. 12: Aspiratewithoutadditionalstroke ................................53
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X Release:09/2008 CyBi®-Well

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CyBi®-Well CyBi®-Well 1
GeneralInformation
1 General Information
1.1 General User Manual Advice
ThisUserManualinformsaboutthesetupandfunctionofCyBi®-Wellsystems.Italso
providesqualifiedoperatingpersonnelwithknowledgerequiredforthesafehandling
ofthesystemanditscomponents.
Furthermore,thisUserManualincludesadviceonsystemmaintenanceandcareaction,
providingreferencestosourcesofpotentialfaultsormalfunctionwithrelatedproposals
forremovaloffaults.
ThisUserManualmustbereadilyavailabletooperatingandmaintenancepersonnelat
alltimes!
Conventions
Work instructions involvingatimedsequencearenumbered,mergedtoactionunits
andspecifyrelatedresults.
Enumerations withoutatimedsequenceareshownasbullet-stylelistings,sub-enu-
merationsasdash-stylelistings.
Safety notesaremarkedwithpictogramsandasignalword(meaningofselectedpic-
togramsandsignalwordssieheKapitel"SafetyNotes").Theyspecifythetype,source
andconsequencesofahazard,givingadviceforaccidentprevention.Asafetynoteal-
waysprecedesaworkinstruction.
Controlandevaluationsoftwareelementsarelabeledasfollows:
Control buttons, menus, description fields, checker boxesandoption fieldsare
showninboldletteringenclosedinbrackets(forexample,[OK]button,[Devices])
menu.
Menuandoptionsequencesareseparatedbyarrow
(e. g.Menu[File] =>[Open]).
Referencestowindowsareshowninitalics(e.g.Measurementwindow).
Field,frameandtabnamesareshowninboldlettering(forexample,Software
tab,Positionframe).
Cross references(internal)aremarkedwithanarrowandshowninitalics.
NOTE
Theinformationcontainedhereinreflectsthelateststateofknowledgeatthemomentof
goingtopress.CyBioAGreservesitsrighttomakechangesifdeemednecessaryinthe
interestoftechnicalprogress.
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2 Release:09/2008 CyBi®-Well
GeneralInformation
1.2 Intended Purpose
TheCyBi®-Wellprovidesasimultaneouspipettingtoolforautomatedprocessingof96-
well,384-wellor1536-wellcapacitymicroplatesinaccordancewithSBSstandardre-
quirements.Itsbasicfunctionsaredosing,pipetting,dispensing,diluting,rinsing,tip
change,transportationandstackingofmicroplates.Optionalsupplementarycapabili-
tiesaree. g.thestockpilingofmicroplates(instackers)andareservoirfillingstation.
TheCyBi®-Wellpipettorisintendedforlaboratoriesdoingresearchanddevelopmentin
thefieldsofpharmacy,biochemistry,clinicalchemistry,combinatorialchemistry,molec-
ularbiology,moleculargeneticsandcellbiology,aswellassuchlaboratorieswherepi-
pettingoperationshavetobecarriedoutinahighlyparallel,rapidandprecisemanner.
Usersareprohibitedfromoperatingthissysteminanywayormodeotherthande-
scribedorspecifiedinthisUserManual.Practicalpipettoroperationisrestrictedbythe
functionalscopeofavailablesoftwareandfirmwareandbythescopeofdelivery.
1.3 Conforming Use
Theterm"conforminguse"oftheCyBi®-Wellpipettorincludesthat:
thesystemisoperatedbyqualifiedandtrainedresearchandlaboratorypersonnel
alloperatingrequirementsquotedinthisUserManualanddescribedprocedural
sequencesandrelatedsafetynotesaredulyobserved
allspecificationsinthisManualregardingsystemstart-up,operation,preventive
maintenanceandcarearemet
applicablesafetystandardsorrulesarealwaysfulfilled.
Thetermnon-conforminguse,typically,includes:
operatingthesysteminmedicallaboratories
workingwithexplosivesubstances
workinginapotentiallyexplosiveatmosphere
Thisimpliestheneedtoobserveallsafetyrequirementsinforceregardingtheprotec-
tionofpersonsandobjectswhereradioactive,infectious,poisonous,corrosive,com-
bustibleandotherhazardoussubstancesarehandled.Itwilltheuser'sresponsibilityto
complywithallrequirementsonlaboratoryequipmentandinstallationsandthecon-
NOTE
Non-conforming use oftheCyBi®-Wellpipettorwillvoidanyclaimforwarrantyorlia-
bilitybyCyBioAGintheeventofmaterialdamageorpersonalinjury!
NOTE
CyBi®-Wellsystemoperationwithinvolvementofdangeroussubstanceswillbethesole
responsibilityoftheuser!
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CyBi®-Well CyBi®-Well 3
GeneralInformation
ductofpersonnelinrespectofmaintainingcleanness,sterilization,environmentalpro-
tectionanddisposalofwaste.
Foroperationwithinvolvementofhazardoussubstances,theuserisrecommendedto
issueinternalworkinstructions.Accordingly,thisUserManualcontainsnosafetynotes
toprotectfrompersonalinjuryormaterialdamagecausedbysubstancesunderexami-
nation.
ProcesscontrolreliesonintegralCyBioAGcontrolsoftware.Changesinordamageto
softwaremayresultinfaultyprocessingsequencesordamagethesystemoritshard-
warecomponents.Softwareprotectionisthesoleresponsibilityoftheuser.
1.4 Warranty & Liability
Theperiodofwarrantyandscopeofliabilitieswillbeasstipulatedunderbindinglaw
andprovidedforintheGeneralTermsofBusinessofCyBioAG.
Warrantywillbelimitedtorepairservicesorreplacementofdamagedparts.Itwillex-
cludeconsequentialdamageofanykind.Damagetowearpartsandcasesofglass
breakagewillnotbecoveredbythiswarranty.
AnydeviationfromconforminguseasdefinedinthisUserManual(operatingrequire-
ments,processsequences)willresultinrestrictedacceptanceofwarrantyorliability
claimsintheeventofdamage.
Intheeventofpersonalinjuryormaterialdamage,claimsforwarrantyorliabilitywillbe
refused,unlesstheCyBi®-Wellcanbeshowntohavebeenoperatedinaccordance
withsection→1.3(→“Conforming Use”).
1.5 Scope of Delivery
CyBi®-Wellsystemdeliveryincludes:
CyBi®-Well
Transportingsystem(lineartypeorcirculartype)
CyBio®Composersoftware
UserManual
Optionalaccessories
NOTE
Thisloss-of-warrantyclauseshallapplytopotentialperiodsofinterruptioninbusinessand
toanysystemcomponentthathadnotbeendirectlyaffectedbyauthorizedwarranty
work.
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4 Release:09/2008 CyBi®-Well
GeneralInformation
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CyBi®-Well Release:09/2008 5
TechnicalData
2TechnicalData
2.1 General Data
Table 1: General data
General characteristics
Designation/Type CyBi®-Well
Numberofpipettetips 96(8x12matrix)or
384(16x24matrix)
Dosablevolumes
1...250μLRandomlyselectableinstepsof0.1μL
0.1...25μLRandomlyselectableinstepsof0.01μL
Precision (variation coefficient CV)
CyBi®-Well96250μLdosinghead
Precisionforvolumerangeof10...25μLVariationcoefficient≤2%
Precisionforvolumerangeof25...250μLVariationcoefficient≤1%
CyBi®-Well9625μLdosinghead
Precisionforvolumerangeof5...10μLVariationcoefficient≤2%
Precisionforvolumerangeof10...25μLVariationcoefficient≤1%
CyBi®-Well38425μLdosinghead
Precisionforvolumerange2...5μLVariationcoefficient≤2%
Precisionforvolumerangeof5...10μLVariationcoefficient≤1%
Lifter
Servolifter HorizontalmotioninX-andY-coordinate
direction
(16positionsonmicroplate)
NOTE
Forthevariationvaluesquotedabove,strictadherencetotheproceduredescribedinsec-
tion→“Precision Test”isacompulsoryprerequisite.
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6 Release:09/2008 CyBi®-Well
TechnicalData
Table 2: Pipetting tips & microplates
Table 3: Dimensional and weight details
Pipetting tips
Single-usetips 250DW,250SW
60μL,25μL,10μLpolypropylene(PP)sin-
gle-usetipsinstandardversion,sterile
PCR-certifiedorAPR-compatiblequality
version
Channels 96or384
Microplate specifications
Formats(SBSstandard) 96-,384-,1536-shallow-wellmicroplates
96-,384-,1536-deep-wellmicroplates
Platepositions
– Linearposition 3,4,5
– Positionsatrotarytable 10
Dimensional & weight details
Widthxheightxdepth
(shallowwell;withouttransportingsys-
tem)
(220x501.5x260)mm
Widthxheightxdepth
(deepwell;withouttransportingsystem)
(220x542.5x260)mm
Widthxheightxdepth
(with3-placelineartransportingsystem)
(960x501.5(542.5)x260)mm
Widthxheightxdepth
(with4-placelineartransportingsystem)
(1300x501.5(542.5)x260)mm
Widthxheightxdepth
(with5-placelineartransportingsystem)
(1650x501.5(542.5)x260)mm
Widthxheightxdepth
(with10-placecirculartransportingsys-
tem)
(700x501.5(542.5)x856)mm
Weight:
–Basicunit About20kg
–Basicunitwithrotarytable About50kg
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CyBi®-Well Release:09/2008 7
TechnicalData
Table 4: Operating data
Operating data
Utilityclass Bench-topdevice,closedroomfacilities
incleancondition
Protectionclass I
Protectiontype IP31
Interferencesuppression EN55011
(DINVDE0875,Part11)
LimitvalueClassA
Interferenceimmunity DINEN61000-6-2
Operatingvoltage 230V±10%,50/60Hz
115V±10%,50/60Hz
Inletfusing 230V:T1,6A
115V:T3,15A
Powerconsumption ≤ 200VA
Airbornesoundemission <75dB(A)
Interface RS232C,Sub-D9-pin
Storage & operating requirements
Storage&transportation:
– Allowableambienttemperature
– Allowablerelativeairhumidity
-10°Cto+50°C
≤ 85%at30°C
Operation:
– Allowableambienttemperature
– Allowablerelativeairhumidity
+15°Cto+35°C
≤ 75%at30°C,noformationofconden-
sate
Conditionoffloorbase Stable,horizontal,dry,freefromvibra-
tion
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8 Release:09/2008 CyBi®-Well
TechnicalData
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CyBi®-Well Release:09/2008 9
SafetyNotes
3SafetyNotes
3.1 General
3.2 Standards & Guidelines
TheCyBi®-Wellhasbeenbuilttomeetcurrentlyvalidrulesoftechnologyandgenerally
establishedrequirementsonsafetyengineering.
Thesystemanditscomponentshavebeendesignedinaccordancewithbasicsafety
andhealthrequirementsinapplicablelaws,standardsandguidelineregulations.CE-la-
bellingandadeclarationofconformity(→refer to page IV)areincludedtodocument
thesafetyofthesystemanditscomponents.
AllstatementsonsafetyarederivedfromcurrentlyvalidregulationsoftheEuropean
Union.Otherspecificnationallawsandregulationsmustbeobserved.
InadditiontothesafetynotesinthisManualandlocalsafetypracticesasmaybeappli-
cabletosystemoperationfromcasetocase,generallyestablishedaccidentprevention,
industriallabourprotectionandenvironmentalprotectionrulesmustbeconsideredand
dulyfollowed.
Noticesconcerningpotentialhazardsdonotreplaceindustrialhealthandsafetyregula-
tionsthatmustbeobservedunderbindinglegislation.
3.3 NRTL Certification
TheCyBi®-Wellhasbeentestedforfunctionalandsafetyfeaturesbyanofficiallyap-
provedcertificationinstitute.Basedonthistest,itqualifiesformarkingwithacertifica-
tionsign.
NOTE
ForyourownsafetyandtoensurefailsafeandreliableCyBi®-Wellsystemoperation,you
shouldcarefullyreadthischapterbeforeproceedingwithanykindofstart-upwork!
Youareadvisedtofollowallsafetynotesthatprecedeadescriptionofrequiredaction
inthevariouschaptersofthisUserManual,andanymessagesoradvisorynoteswhich
areoutputbycontrolandevaluationsoftwarefordisplayingonthemonitorscreen.
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