cytiva EPS 301 User manual

Electrophoresis Power Supply
EPS 301
Operating Instructions
Original instructions
cytiva.com

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Table of Contents
1 Introduction ........................................................................................................ 5
1.1 About this manual ......................................................................................................................................... 6
1.2 Important user information ....................................................................................................................... 7
1.3 Associated documentation ....................................................................................................................... 9
2 Safety instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels ................................................................................................................................................................. 17
2.3 Emergency procedures ............................................................................................................................... 18
3 System description ............................................................................................ 19
3.1 Materials ........................................................................................................................................................... 20
3.2 Front panel ....................................................................................................................................................... 21
3.3 Rear panel ......................................................................................................................................................... 23
4 Unpacking and installation ................................................................................ 24
5 Operation ............................................................................................................ 27
5.1 Connecting the electrophoresis unit(s) ................................................................................................ 29
5.2 Programming .................................................................................................................................................. 30
5.3 Running .............................................................................................................................................................. 31
5.4 Quick guide ....................................................................................................................................................... 33
6 Maintenance ....................................................................................................... 36
7 Troubleshooting ................................................................................................. 38
8 Reference information ....................................................................................... 39
8.1 Technical specification ................................................................................................................................ 40
8.2 Recycling information .................................................................................................................................. 42
8.3 Regulatory information ............................................................................................................................... 43
8.3.1 Contact information .................................................................................................................................... 44
8.3.2 European Union and European Economic Area .............................................................................. 45
8.3.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 46
8.3.4 Regulations for North America ............................................................................................................... 48
8.3.5 Regulatory statements .............................................................................................................................. 49
8.3.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 50
8.3.7 Other regulations and standards .......................................................................................................... 52
8.4 Ordering information ................................................................................................................................... 53
8.5 Health and Safety Declaration Form ...................................................................................................... 54
Index ........................................................................................................................... 56
Table of Contents
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 3

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1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, and intended use of Electrophoresis Power Supply EPS 301.
In this chapter
Section See page
1.1 About this manual 6
1.2 Important user information 7
1.3 Associated documentation 9
1 Introduction
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 5

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover the use of the Electrophoresis Power Supply EPS 301
instrument.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
1 Introduction
1.1 About this manual
6 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
The Electrophoresis Power Supply EPS 301 is a high quality, high precision power
supply for electrophoresis applications that require a maximum of 300 V, 400 mA and
80 W. EPS 301 is primarily designed for the following techniques:
1. Submarine DNA electrophoresis
2. Electroblotting
3. Immuno electrophoresis
4. Mini polyacrylamide gel electrophoresis
Electrophoresis separations can be controlled by voltage or current. The EPS 301 auto-
matically switches over the controlling parameter according to programmed limits
and conductivity variations in the system. Two electrophoresis units can be connected
to the EPS 301 and run with the same programmed method at one time.
The Electrophoresis Power Supply EPS 301 is intended for research use only, and shall
not be used in any clinical procedures, or for diagnostic purposes.
WARNING
Do not operate the product in any other way than described in the
user documentation.
Prerequisites
In order to operate EPS 301 safely and according to the intended purpose, the following
prerequisites must be fulfilled:
1 Introduction
1.2 Important user information
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 7

•You must have read and understood the safety instructions in Chapter 2 Safety
instructions, on page 10 of these Operating Instructions.
•You should be acquainted with the use of general laboratory equipment and
handling of biological materials.
•You should understand the concepts of electrophoresis.
•The system should be installed according to the instructions in Chapter 4
Unpacking and installation, on page 24 of these Operating Instructions.
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
8 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB

1.3 Associated documentation
System-specific documentation
In addition to the Operating Instructions manual, the documentation package supplied
with the product also includes product documentation binders containing detailed
specifications and traceability documents.
The most important documents in the document package with regard to technical
aspects of the product are:
Document Abbreviation Purpose/Contents
Bill of Material BOM Detailed specifications for process related
components.
Component documentation
Documentation for components produced both by Cytiva and by a third-party are, if
existent, also included in the product documentation.
1 Introduction
1.3 Associated documentation
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 9

2 Safety instructions
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels 17
2.3 Emergency procedures 18
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures,
and provides recycling information.
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
2 Safety instructions
10 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB

2.1 Safety precautions
Introduction
Electrophoresis Power Supply EPS 301 is powered by mains voltage and handles mate-
rials that may be hazardous. Before installing, operating or maintaining the system,
you must be aware of the hazards described in this manual.
Follow the instructions provided to avoid personal injuries, damage to the
product, or to other personnel and equipment in the area.
The safety precautions in this section are grouped in the following categories:
•General precautions, on page 11
•Flammable liquids and explosive environment, on page 13
•Personal protection, on page 13
•Installing and moving, on page 13
•Power supply, on page 13
•Operation, on page 15
•Maintenance, on page 15
General precautions
WARNING
Risk assessment. Perform a risk assessment for any risks due to
the process or process environment. Evaluate the effects the use of
the product and the operational processes may have on the classi-
fication of the hazardous area. The process might cause the area to
increase or the zone classification to change. Implement the risk
reduction measures needed, including use of personal protective
equipment.
WARNING
Always follow these General Precautions to avoid injury when using
EPS 301.
WARNING
Operation and user maintenance of EPS 301 should be performed
by properly trained personnel only.
2 Safety instructions
2.1 Safety precautions
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 11

WARNING
Protective earth. The product must always be connected to
protective earth when energized.
WARNING
This instrument can develop sufficient voltage and current to
produce a lethal shock. Extreme caution should be exercised in its
operation.
WARNING
Check electrical cables for damage and the electrophoresis unit for
damage or leakage before use. If damage and/or leakage is
present, do not use the equipment. There is a risk for high voltage
injuries.
WARNING
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
CAUTION
The product is designed for indoor use only.
NOTICE
If the power supply is connected to 200 to 240 V with the range set
to 100 to 120 V, the instrument can be severely damaged.
2 Safety instructions
2.1 Safety precautions
12 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB

Flammable liquids and explosive
environment
WARNING
Explosive environment. The product is not approved for work in
a potentially explosive atmosphere. The product does not fulfill the
requirements of the ATEX Directive.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
Installing and moving
WARNING
Do not block the ventilation inlets or outlets on the system.
CAUTION
Do not use the product in a dusty atmosphere or close to spraying
water.
Power supply
WARNING
If liquid is spilled on the equipment, the electrical power supply
must be disconnected immediately. The equipment must be
completely dry on the inside and the outside before reconnecting
the power supply.
WARNING
Only use power cables delivered or approved by Cytiva.
2 Safety instructions
2.1 Safety precautions
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 13

WARNING
Use only undamaged electrical cables and equipment approved for
the voltage you will use. When replacing a damaged electrical
cable, use a replacement cable of the same type and dimensions.
High voltage electrical cables should be in accordance with
IEC/EN/UL/CSA 61010-1. All equipment connected to high voltage
electrical cables should be in accordance with IEC/EN/UL/CSA
61010-1 or other relevant standard.
WARNING
Local regulation for Great Britain
This appliance must be earthed.
The wires in the mains lead are colored in accordance with the
following code:
Green and yellow Earth
Blue Neutral
Brown Live
If the plug provided is unsuitable for your socket outlets, the plug
must be cut off and a suitable plug fitted. The cut-off plug should be
disposed of and must not be inserted into any socket as this can
result in electric shock. The plug or adapter or the distribution
panel should be provided with a 13 amp fuse. As the colors of the
wires in the mains lead of this appliance may not correspond with
colored markings identifying the terminals in your plug, proceed as
follows:
The green and yellow wire must be connected to the terminal in the
plug which is marked with the letter E or by the earth symbol, or
colored green, or green and yellow.
The blue wire must be connected to the terminal which is marked
with the letter N or colored black.
The brown wire must be connected to the terminal which is marked
with the letter L or colored red.
Note:
After replacing or changing a fuse, the fuse cover in the plug must
be replaced with a fuse cover which corresponds to the colour of
the insert in the base of the plug or the word that is embossed on
the base of the plug, and the appliance must not be used without a
fuse cover.
Only 13 Amp fuses approved to B.S. 1362 A.S.T.A. should be used.
2 Safety instructions
2.1 Safety precautions
14 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB

NOTICE
If the power supply is connected to 200 to 240 V with the range set
to 100 to 120 V, the instrument can be severely damaged.
Operation
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Do not operate the instrument in extreme humidity (above 95%).
Avoid condensation by letting the unit equilibrate to ambient
temperature when taking EPS 301 from a colder to a warmer envi-
ronment.
Maintenance
WARNING
The instrument covers must not be opened by the user. The instru-
ment contains electrical circuits which can give a lethal electric
shock. Service and planned maintenance shall be performed by
personnel authorized by Cytiva.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
2 Safety instructions
2.1 Safety precautions
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 15

NOTICE
Cleaning. Keep the exterior of the instrument dry and clean. Wipe
regularly with a soft damp tissue and, if necessary, a mild cleaning
agent. Let the instrument dry completely before use.
2 Safety instructions
2.1 Safety precautions
16 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB

2.2 Labels
Introduction
This section describes the system label and other safety labels that are attached to the
product.
System label
The system label is located on the back of the equipment. The system label identifies
the equipment and shows electrical data, regulatory compliance, and warning
symbols.
Label text Description
Warning! Read the user documentation before using the
system. Do not open any covers or replace parts unless
specifically stated in the user documentation.
Code no Ordering number for EPS 301
Serial no System serial number
Mfg. year Year (YYYY) and month (MM) of manufacture
Voltage Supply voltage
Frequency Supply voltage frequency
Max Power Max power consumption
Safety labels
The table below describes the various safety labels that may be found on the product.
Symbol/text Description
Warning! Read the user documentation before using the
system. Do not open any covers or replace parts unless specifi-
cally stated in the user documentation.
2 Safety instructions
2.2 Labels
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 17

2.3 Emergency procedures
Introduction
This section describes how to perform an emergency shutdown of the product, the
result in the event of power failure, and the procedure for restarting the product after a
power failure.
Precautions
WARNING
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
Emergency shutdown
In an emergency situation, switch off the power to the instrument by pressing the
Power switch to the 0 position. If required, disconnect the mains power cord. The run is
interrupted immediately.
Restart after emergency shutdown or
power failure
Power failure to Electrophoresis Power Supply EPS 301 will cause the run to stop
immediately in an undefined state.
Turn on the mains power if Electrophoresis Power Supply EPS 301 is switched off. The
program continues when power is resumed. The message -PF- can be removed by
pressing any key. No other special actions are needed.
2 Safety instructions
2.3 Emergency procedures
18 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB

3 System description
About this chapter
This chapter gives an overview of the Electrophoresis Power Supply EPS 301, and a
brief description of its function.
In this chapter
Section See page
3.1 Materials 20
3.2 Front panel 21
3.3 Rear panel 23
3 System description
Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB 19

3.1 Materials
Materials
The main components in the EPS 301 Power Supply are made of the following mate-
rials.
Component Material
Instrument box Aluminium
Display holder Polypropene (PP). UL94V0
Keyboard Polyester
Output sockets Acetalplastic (POM). UL94V0
Rubber feet Polyurethane
3 System description
3.1 Materials
20 Electrophoresis Power Supply EPS 301 Operating Instructions 29115049 AB