cytiva EPS 3501 XL User manual

Electrophoresis Power Supply
EPS 3501 XL
Operating Instructions
Original instructions
Translation disc included
cytiva.com

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Table of Contents
1 Introduction ........................................................................................................ 5
1.1 Important user information ....................................................................................................................... 6
1.2 About this manual ......................................................................................................................................... 8
1.3 Associated documentation ....................................................................................................................... 9
2 Safety instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels ................................................................................................................................................................. 16
2.3 Emergency procedures ............................................................................................................................... 18
2.4 Built-in safety features ................................................................................................................................ 19
3 System description ............................................................................................ 20
3.1 Instrument ........................................................................................................................................................ 21
3.2 Front panel ....................................................................................................................................................... 22
3.3 Rear panel ......................................................................................................................................................... 26
4 Installation .......................................................................................................... 27
4.1 Transport and unpacking ............................................................................................................................ 29
4.2 Electrical connections ................................................................................................................................. 31
5 Operation ............................................................................................................ 32
5.1 Starting the instrument ............................................................................................................................... 34
5.2 Setting up a run ............................................................................................................................................... 35
5.2.1 Entering a program ...................................................................................................................................... 36
5.2.2 Editing a program ......................................................................................................................................... 41
5.2.3 Optional programming .............................................................................................................................. 43
5.2.4 Choosing run parameters ......................................................................................................................... 45
5.3 Performing a run ............................................................................................................................................ 48
5.4 Quick guide ....................................................................................................................................................... 52
6 Maintenance ....................................................................................................... 54
7 Troubleshooting ................................................................................................. 56
8 Reference information ....................................................................................... 58
8.1 Specifications .................................................................................................................................................. 59
8.2 Ordering information ................................................................................................................................... 61
8.3 Recycling information .................................................................................................................................. 62
8.4 Regulatory information ............................................................................................................................... 63
8.4.1 Contact information .................................................................................................................................... 64
8.4.2 European Union and European Economic Area .............................................................................. 65
8.4.3 Great Britain ................................................................................................................................................... 66
8.4.4 Eurasian Economic Union (Евразийский экономический союз) .......................................... 67
8.4.5 North America ............................................................................................................................................... 69
8.4.6 Regulatory statements .............................................................................................................................. 70
8.4.7 Declaration of Hazardous Substances (DoHS) ................................................................................ 71
8.4.8 Other regulations and standards .......................................................................................................... 73
Table of Contents
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 3

8.5 Health and Safety Declaration Form ...................................................................................................... 74
Index ........................................................................................................................... 76
Table of Contents
4 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

1 Introduction
About this chapter
This chapter contains important user information, and intended use of Electrophoresis
Power Supply EPS 3501 XL.
In this chapter
Section See page
1.1 Important user information 6
1.2 About this manual 8
1.3 Associated documentation 9
1 Introduction
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 5

1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating, or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use of the product
Electrophoresis Power Supply EPS 3501 XL is a power supply for electrophoresis appli-
cations that require advanced programming and high voltage. EPS 3501 XL is primarily
designed for techniques using programming in several phases and/or voltage gradi-
ents:
•2-D (two dimensional) electrophoresis using Multiphor II Electrophoresis System
•IEF (Isoelectric focusing) using Immobiline™ DryStrip gels or CleanGel IEF
•Flatbed SDS-PAGE (Polyacrylamide Gel Electrophoresis) or flatbed native PAGE
using e.g., ExcelGel™
EPS 3501 XL is also suitable for:
•DNA sequencing
•Agarose electrophoresis
•Electroblotting
•DNA pulsed field electrophoresis
EPS 3501 XL is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate EPS 3501 XL in a safe way and according to the intended purpose,
the following prerequisites must be fulfilled:
•You must have read and understood the safety instructions in Chapter 2 Safety
instructions, on page 10 of these Operating Instructions.
1 Introduction
1.1 Important user information
6 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

•You must be acquainted with the use of general laboratory equipment and with
handling of biological materials.
•You must understand the concepts of electrophoresis.
•The product should be installed according to the instructions in Chapter 4 Installa-
tion, on page 27 of these Operating Instructions.
1 Introduction
1.1 Important user information
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 7

1.2 About this manual
Introduction
This section contains information about the purpose and scope of this manual, notes
and tips, and typographical conventions.
Purpose of this manual
This manual provides you with the information needed to install, operate, and maintain
the product in a safe way.
Scope of this manual
This manual is valid for Electrophoresis Power Supply EPS 3501 XL. The image below
shows the product.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
Tip: The text can include clickable hyperlinks to reference information.
1 Introduction
1.2 About this manual
8 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

1.3 Associated documentation
System specific documentation
The documentations are listed in the table below.
Document Main contents
Electrophoresis Power
Supply EPS 3501 XL Oper-
ating Instructions
(29115054)
(this document)
Instructions needed to prepare and operate the
product in a correct and safe way.
System overview, site requirements, and instruc-
tions for moving the product within the same
building.
Instructions for basic maintenance and trouble-
shooting.
Translation CD (29196242) Contains translations of the operating instructions
Bill of Material (BOM) Detailed specifications for process related compo-
nents.
In addition to above documentations, the product package also include product docu-
mentation binders containing detailed specifications and traceability documents.
Component documentation
Documentation for components produced both by Cytiva and by a third-party are, if
existent, also included in the product documentation.
1 Introduction
1.3 Associated documentation
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 9

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
product. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels 16
2.3 Emergency procedures 18
2.4 Built-in safety features 19
Important
WARNING
All users must read and understand the entire contents of
this general safety chapter, and the specific safety precau-
tions information in each subsequent chapter of this manual
to become aware of the hazards involved.
2 Safety instructions
10 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC