cytiva EPS 3501 XL User manual

Electrophoresis Power Supply
EPS 3501 XL
Operating Instructions
Original instructions
Translation disc included
cytiva.com

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Table of Contents
1 Introduction ........................................................................................................ 5
1.1 Important user information ....................................................................................................................... 6
1.2 About this manual ......................................................................................................................................... 8
1.3 Associated documentation ....................................................................................................................... 9
2 Safety instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels ................................................................................................................................................................. 16
2.3 Emergency procedures ............................................................................................................................... 18
2.4 Built-in safety features ................................................................................................................................ 19
3 System description ............................................................................................ 20
3.1 Instrument ........................................................................................................................................................ 21
3.2 Front panel ....................................................................................................................................................... 22
3.3 Rear panel ......................................................................................................................................................... 26
4 Installation .......................................................................................................... 27
4.1 Transport and unpacking ............................................................................................................................ 29
4.2 Electrical connections ................................................................................................................................. 31
5 Operation ............................................................................................................ 32
5.1 Starting the instrument ............................................................................................................................... 34
5.2 Setting up a run ............................................................................................................................................... 35
5.2.1 Entering a program ...................................................................................................................................... 36
5.2.2 Editing a program ......................................................................................................................................... 41
5.2.3 Optional programming .............................................................................................................................. 43
5.2.4 Choosing run parameters ......................................................................................................................... 45
5.3 Performing a run ............................................................................................................................................ 48
5.4 Quick guide ....................................................................................................................................................... 52
6 Maintenance ....................................................................................................... 54
7 Troubleshooting ................................................................................................. 56
8 Reference information ....................................................................................... 58
8.1 Specifications .................................................................................................................................................. 59
8.2 Ordering information ................................................................................................................................... 61
8.3 Recycling information .................................................................................................................................. 62
8.4 Regulatory information ............................................................................................................................... 63
8.4.1 Contact information .................................................................................................................................... 64
8.4.2 European Union and European Economic Area .............................................................................. 65
8.4.3 Great Britain ................................................................................................................................................... 66
8.4.4 Eurasian Economic Union (Евразийский экономический союз) .......................................... 67
8.4.5 North America ............................................................................................................................................... 69
8.4.6 Regulatory statements .............................................................................................................................. 70
8.4.7 Declaration of Hazardous Substances (DoHS) ................................................................................ 71
8.4.8 Other regulations and standards .......................................................................................................... 73
Table of Contents
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 3

8.5 Health and Safety Declaration Form ...................................................................................................... 74
Index ........................................................................................................................... 76
Table of Contents
4 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

1 Introduction
About this chapter
This chapter contains important user information, and intended use of Electrophoresis
Power Supply EPS 3501 XL.
In this chapter
Section See page
1.1 Important user information 6
1.2 About this manual 8
1.3 Associated documentation 9
1 Introduction
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 5

1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating, or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use of the product
Electrophoresis Power Supply EPS 3501 XL is a power supply for electrophoresis appli-
cations that require advanced programming and high voltage. EPS 3501 XL is primarily
designed for techniques using programming in several phases and/or voltage gradi-
ents:
•2-D (two dimensional) electrophoresis using Multiphor II Electrophoresis System
•IEF (Isoelectric focusing) using Immobiline™ DryStrip gels or CleanGel IEF
•Flatbed SDS-PAGE (Polyacrylamide Gel Electrophoresis) or flatbed native PAGE
using e.g., ExcelGel™
EPS 3501 XL is also suitable for:
•DNA sequencing
•Agarose electrophoresis
•Electroblotting
•DNA pulsed field electrophoresis
EPS 3501 XL is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate EPS 3501 XL in a safe way and according to the intended purpose,
the following prerequisites must be fulfilled:
•You must have read and understood the safety instructions in Chapter 2 Safety
instructions, on page 10 of these Operating Instructions.
1 Introduction
1.1 Important user information
6 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

•You must be acquainted with the use of general laboratory equipment and with
handling of biological materials.
•You must understand the concepts of electrophoresis.
•The product should be installed according to the instructions in Chapter 4 Installa-
tion, on page 27 of these Operating Instructions.
1 Introduction
1.1 Important user information
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 7

1.2 About this manual
Introduction
This section contains information about the purpose and scope of this manual, notes
and tips, and typographical conventions.
Purpose of this manual
This manual provides you with the information needed to install, operate, and maintain
the product in a safe way.
Scope of this manual
This manual is valid for Electrophoresis Power Supply EPS 3501 XL. The image below
shows the product.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
Tip: The text can include clickable hyperlinks to reference information.
1 Introduction
1.2 About this manual
8 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

1.3 Associated documentation
System specific documentation
The documentations are listed in the table below.
Document Main contents
Electrophoresis Power
Supply EPS 3501 XL Oper-
ating Instructions
(29115054)
(this document)
Instructions needed to prepare and operate the
product in a correct and safe way.
System overview, site requirements, and instruc-
tions for moving the product within the same
building.
Instructions for basic maintenance and trouble-
shooting.
Translation CD (29196242) Contains translations of the operating instructions
Bill of Material (BOM) Detailed specifications for process related compo-
nents.
In addition to above documentations, the product package also include product docu-
mentation binders containing detailed specifications and traceability documents.
Component documentation
Documentation for components produced both by Cytiva and by a third-party are, if
existent, also included in the product documentation.
1 Introduction
1.3 Associated documentation
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 9

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
product. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels 16
2.3 Emergency procedures 18
2.4 Built-in safety features 19
Important
WARNING
All users must read and understand the entire contents of
this general safety chapter, and the specific safety precau-
tions information in each subsequent chapter of this manual
to become aware of the hazards involved.
2 Safety instructions
10 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

2.1 Safety precautions
Introduction
EPS 3501 XL is powered by mains voltage and handles materials that may be
hazardous. Before installing, operating or maintaining the product, you must be aware
of the hazards described in this manual.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
General precautions
WARNING
Risk assessment. Perform a risk assessment for any risks due to
the process or process environment. Evaluate the effects the use of
the product and the operational processes may have on the classi-
fication of the hazardous area. The process might cause the area to
increase or the zone classification to change. Implement the risk
reduction measures needed, including use of personal protective
equipment.
2 Safety instructions
2.1 Safety precautions
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 11

WARNING
Always follow these General Precautions to avoid injury when using
EPS 3501 XL.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Protective earth. The product must always be connected to
protective earth when energized.
WARNING
Lethal electric shock. This instrument can develop sufficient
voltage and current to produce a lethal shock. Extreme caution
must be exercised in its operation.
WARNING
Check electrical cables for damage and the electrophoresis unit for
damage or leakage before use. If damage and/or leakage is
present, do not use the equipment. There is a risk for high voltage
injuries.
WARNING
Access to power switch and power cord. The power switch
must always be easy to access. The power cord must always be
easy to disconnect.
CAUTION
The product is designed for indoor use only.
2 Safety instructions
2.1 Safety precautions
12 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

NOTICE
If the power supply is connected to 200 to 240 V with the range set
to 100 to 120 V, the instrument can be severely damaged.
Flammable liquids and explosive
environment
WARNING
Explosive environment. The product is not approved for work in
a potentially explosive atmosphere, in areas classified as Zone 0 to
Zone 2 according to IEC 60079-10 2002. The product does not fulfill
the requirements of the ATEX Directive.
Personal protection
WARNING
Always use appropriate personal protective equipment during
operation and maintenance of the product.
Installing and moving
WARNING
Do not block the ventilation inlets or outlets on the system.
CAUTION
Do not use the product in a dusty atmosphere or close to spraying
water.
2 Safety instructions
2.1 Safety precautions
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 13

Power supply
WARNING
If liquid is spilled on the equipment, the electrical power supply
must be disconnected immediately. The equipment must be
completely dry on the inside and the outside before reconnecting
the power supply.
WARNING
Only use power cables delivered or approved by Cytiva.
WARNING
Use only undamaged electrical cables and equipment approved for
the voltage you will use. When replacing a damaged electrical
cable, use a replacement cable of the same type and dimensions.
High voltage electrical cables should be in accordance with
IEC/EN/UL/CSA 61010-1. All equipment connected to high voltage
electrical cables should be in accordance with IEC/EN/UL/CSA
61010-1 or other relevant standard.
NOTICE
If the power supply is connected to 200 to 240V with the range set
to 100 to 120V, the instrument can be severely damaged.
Operation
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
2 Safety instructions
2.1 Safety precautions
14 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

WARNING
Do not operate the instrument in extreme humidity (above 95%).
Avoid condensation by letting the unit equilibrate to ambient
temperature when taking EPS 3501 XL from a colder to a warmer
environment.
WARNING
By disabling the start current check, the power supply can deliver
high voltage even if it is not connected to electrophoresis equip-
ment.
Maintenance
WARNING
The instrument covers must not be opened by the user. The instru-
ment contains electrical circuits which can give a lethal electric
shock. Service and planned maintenance shall be performed by
personnel authorized by Cytiva.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
NOTICE
Cleaning. Keep the exterior of the instrument dry and clean. Wipe
regularly with a soft damp tissue and, if necessary, a mild cleaning
agent. Let the instrument dry completely before use.
2 Safety instructions
2.1 Safety precautions
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 15

2.2 Labels
Introduction
This section describes the system label and other safety labels that are attached to the
product.
System label
The system label is located on the back of EPS 3501 XL. The system label identifies the
product and shows electrical data, regulatory compliance, and warning symbols.
Label text Description
Warning! Read the user documentation before using the
system. Do not open any covers or replace parts unless specifi-
cally stated in the user documentation.
This symbol indicates that the waste of electrical and elec-
tronic equipment must not be disposed as unsorted municipal
waste and must be collected separately. Please contact an
authorized representative of the manufacturer for information
concerning the decommissioning of equipment.
Code no Ordering number for the product.
Serial no System serial number
Mfg. year Year (YYYY) and month (MM) of manufacture
Voltage Electrical rating:
Voltage (VAC )
Frequency Electrical rating: Frequency (Hz)
Max Power Electrical rating: Max. power (VA)
Safety labels
The table below describes the various safety labels that may be found on the product.
Symbol/text Description
Warning! Read the user documentation before using the
system. Do not open any covers or replace parts unless specifi-
cally stated in the user documentation.
2 Safety instructions
2.2 Labels
16 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

Symbol/text Description
Warning! High Voltage. Always make sure that the system is
disconnected from electric power before opening the cabinet
doors or disconnecting any electric device.
2 Safety instructions
2.2 Labels
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 17

2.3 Emergency procedures
Introduction
This section describes how to perform an emergency shutdown of the product, the
result in the event of power failure, and the procedure for restarting the product after a
power failure.
Precautions
WARNING
Access to power switch and power cord. The power switch
must always be easy to access. The power cord must always be
easy to disconnect.
Emergency shutdown
In an emergency situation, switch off the power to the instrument by pressing the
Power switch to the 0 position. If required, disconnect the mains power cord. The run is
interrupted immediately.
Restart after emergency shutdown or
power failure
Power failure to EPS 3501 XL will cause the run to stop immediately in an undefined
state.
Turn on the mains power if EPS 3501 XL is switched off. The program continues when
power is resumed.
2 Safety instructions
2.3 Emergency procedures
18 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC

2.4 Built-in safety features
The power supply has the following built-in safety functions:
Function Description
Functional earth
leakage
If the power supply is connected to an electrophoresis
unit that has a leakage path to earth, EPS 3501 XL will
detect this fault and turn off the high voltage.
Start current check EPS 3501 XL checks that the resistance is not higher than
a specified limit at a low safety voltage (<40V). If this
resistance is too high, the high voltage is turned off.
Too high resistance can be caused by for example:
•an electrophoresis unit incorrectly connected
•use of buffer with extremely low conductivity
This function can be disabled to perform certain applica-
tions, see Disabling the start current check, on page 40.
Sudden load change
detection
This function prevents accidents under running condi-
tions due to a break in the electrical circuit such as a bad
connection to the electrophoresis unit. The high voltage
is turned off in such an event. An error message is shown
on the display in the event of a sudden load change.
2 Safety instructions
2.4 Built-in safety features
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC 19

3 System description
About this chapter
This chapter gives an overview of EPS 3501 XL, and a brief description of its function.
In this chapter
Section See page
3.1 Instrument 21
3.2 Front panel 22
3.3 Rear panel 26
3 System description
20 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC