D FASTER CytoFAST Top Operating instructions

00055EN - Rev. 10 – 12/2011 – (ORIGINAL INSTRUCTIONS)
Commercial office:
Via Merendi, 22
20010 Cornaredo (MI)
Tel. [phone]
Fax. [phone]
E-mail: [e-mail]
OPERATING AND
MAINTENANCE MANUAL
CytoFAST Top
VERTICAL LAMINAR AIRFLOW CABINET
FOR CYTOTOXIC DRUGS

a D:GROUP Company
00055EN Rev.10 – 12/2011 1
CONTENTS
1 GENERAL.................................................................................................................................................................. 2
2 INSTALLATION.......................................................................................................................................................... 3
2.A INSTRUCTIONS AND CHECKS ON DELIVERY ............................................................................................... 3
2.B INSTALLATION REQUIREMENTS.................................................................................................................... 3
2.C ELECTRIC/GAS CONNECTIONS AND INSTALLATION OF THE WORK SURFACE......................................... 5
3 OPERATION PRINCIPLES........................................................................................................................................ 7
4 OPERATION .............................................................................................................................................................. 8
4.A SCOPE.............................................................................................................................................................. 8
4.B CONTROL AND REGULATION SYSTEMS....................................................................................................... 8
4.C REMOTE SIGNALS (OPTIONAL)...................................................................................................................... 9
4.D SYMBOLS OF THE CONTROL PANEL.............................................................................................................. 10
4.E MANAGEMENT AND PROGRAMMING OF RESIDUAL LIFETIME ................................................................. 14
4.F DISPOSAL OF WASTES AND CONTAMINATED MATERIALS....................................................................... 19
5 LIMITATIONS........................................................................................................................................................... 20
6 OPERATING PROCEDURES.................................................................................................................................. 21
6.A PRELIMINARY CHECKS................................................................................................................................. 21
6.B SWITCHING ON THE C YTO FAST T OP CABINET .................................................................................................. 21
6.C SWITCHING OFF THE C YTO FAST T OP CABINET ................................................................................................ 21
7 MAINTENANCE ....................................................................................................................................................... 22
7.A INSTRUCTIONS FOR DAILY CLEANING OF C YTO FAST T OP ( BY USERS )........................................................ 22
7.B INSTRUCTIONS FOR THE FUMIGATION/STERILIZATION OF BIOLOGIC SAFETY CABINETS .................. 23
7.C REPLACEMENT OF HEPA FILTERS ( BY TECHNICAL ASSISTANCE PERSONNEL )................................................... 26
7.D REPLACEMENT OF MOTOR-FANS ( BY TECHNICAL ASSISTANCE PERSONNEL ).................................................... 27
7.E REPLACEMENT OF FLUORESCENT LAMPS ( BY USER )................................................................................. 28
7.F REPLACEMENT OF UV GERMICIDE LAMP ( OPTIONAL - BY USER )................................................................... 28
7.G SPARE PARTS LIST........................................................................................................................................ 29
8 TROUBLESHOOTING - PROBABLE CAUSES OF MALFUNCTIONS .................................................................... 30
9 MONITORING SYSTEM........................................................................................................................................... 32
10 TECHNICAL SPECIFICATIONS.......................................................................................................................... 33
11 TRANSPORT, PACKING and STORAGE INSTRUCTIONS................................................................................ 34
12 ADDITIONAL INFORMATION.............................................................................................................................. 35
12.A GUARANTEE .................................................................................................................................................. 35
12.B ADDRESS FOR TECHNICAL ASSISTANCE ( FOR THE DISTRIBUTOR )................................................................ 35
13 DIAGRAMS.......................................................................................................................................................... 36
13.A DIAGRAM FOR MAINTENANCE OPERATIONS............................................................................................. 37
13.B C YTO FAST T OP FRONTAL AND SIDE DIAGRAM.............................................................................................. 39
13.C SAFE WORKING AREA DIAGRAM................................................................................................................. 41
14 SENSORS LIST................................................................................................................................................... 42
15 WIRING DIAGRAM.............................................................................................................................................. 43
16 PROCEDURE FOR CytoFAST Top ASSEMBLY................................................................................................. 46
17 DECLARATION OF CONFORMITY..................................................................................................................... 47
REFERENCE STANDARD: this instruction and maintenance manual complies with the European standard en-591 (march
1994) approved by CEN (European Committee for Standardization) relating to the requirements for drawing up users'
instruction manuals for laboratory instruments.

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00055EN Rev.10 – 12/2011 2
1 GENERAL
Vertical laminar airflow benches with partial exhaust and protection barrier, the CytoFAST Top cabinets, are
designed to protect both the material to be manipulated from contamination and to protect the operator and
the environment from microbial cytotoxic contamination hazards.
The de-dusted, filtered and sterile air passing through the main HEPA filter ensures optimum airflow
laminarity on the work surface, thanks to its even perforation and the frontal air barrier. The system is
designed to create a protective barrier and prevent exchanges between the internal, potentially biologically
contaminated air, and the outside.
CytoFAST Top cabinets are Class II Biohazard cabinets, according to the definitions now adopted by all the
main international standards, with about 70% laminar flow recirculated air and 30% extracted air with prior
HEPA filtering. Suitable for the treatment of class CDC-1/2/3, DHSS-C/B1/B2 group 1,2,3 microorganisms as
per D.L. 81/08 “Safety in the working environment” and wherever a product-personnel-environment cross-
protection is required.
Especially suitable for applications such as:
- Manipulation of eziologic agents having a known pathogenicity on humans and animals.
- Presence of high concentrations of biologic materials.
- Presence of agents likely to cause genetic alterations or synergetic activities with other materials.
- Oncogenic viruses.
But above all designed for:
- Preparation and handling of cytotoxic drugs
- Preparation and manipulation of antineoplastic chemotherapeutics
Do not manipulate the products on the slotted part at the entry of the chamber, but in the middle of the work
surface.
The performances of the cabinets are detailed in the TESTING CERTIFICATE below, in compliance with the
requirements of:
- ISO 14644-1 Class 5
For the Biohazard/Cytotoxicity:
- DIN 12980: 2005
- EN 12469: 2000
Furthermore CytoFAST Top cabinets fulfil the armonized standards EN 61010-1 as well as EN 61326
according to the applicable European directives regarding the CE marking.
CytoFAST Top cabinets comply with the above-mentioned standards ONLY if the instruments connected to
the electrical socket positioned inside the work chamber are "CE" marked or in any case it meets the above
mentioned standards aiming to avoid any electromagnetic interference.
All FASTER's cabinets are provided with high insertion loss filters.
Faster s.r.l. cannot be held responsible for malfunctions, damage to people or property due to non-
compliance, poor or no maintenance, or improper use of the cabinet.

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00055EN Rev.10 – 12/2011 3
2 INSTALLATION
2.A INSTRUCTIONS AND CHECKS ON DELIVERY
Considering the critical nature of the use of the CytoFAST Top cabinet and the need to keep it in optimum
condition, installation is very important.
CytoFAST Top Biohazard cabinets are positioned on a pallet, wrapped in an extensible film and contained in
a package of multi-layer strapped cardboard.
After placing the cabinet in its site of use, opened the package and removed the extensible film, check that
the equipment has not suffered any dents or scratches due to transport or improper handling of the package.
In case of any further transport, packing and storage by the user after the initial period of use (e.g.: change
of laboratory or factory), contact the technical assistance service or the distributor for more accurate and
precise instructions or for assistance by specialized technicians.
2.B INSTALLATION REQUIREMENTS
Install the cabinet away from drafts and heat sources (radiators, ventilators/convectors), to ensure proper
functioning. For instance, in a small room (<30 m
3
), if an exhaust duct is used to discharge air outside the
building, we recommend the installation of a grill in the room in order to provide an air supply equal at least to
the quantity entering the front of the cabinet to create the protective barrier and a negative pressure in the
work area.
- Install the cabinet in a well-ventilated room with a low degree of dust.
- The distance between the cabinet and the exhaust system should be as short as possible.
- Place the cabinet away from doors and windows, which may cause malfunctions
- Place the cabinet in places where there is little human traffic.
- The exhaust system should be installed on the roof or on a wall or through a window. Otherwise, it can be
connected to a chimney.
- The door of the room should be in such a position relative to the cabinet as to prevent drafts.
- The door of the room should be in such a position relative to the cabinet as to prevent drafts.
oMin. temperature 5 °C
oMax. temperature: 40 °C
oMax. humidity: 80% at 31°C, linear drop in relative humidity down to 50% relative
humidity at 40°C.
It is recommended that the exhaust duct, located on top of the cabinet, should be connected to the outside of
the building. In this case an anti-blowback shutter should also be installed on the exhaust flange.
The max. length of the ∅200mm exhaust duct should not exceed 10m. Otherwise changes must be made in
the extraction fan or a remote exhaust motor-fan must be installed. The anti-blowback system prevents air
from flowing back into the extraction channel.
Before connecting the cabinet to the mains power supply, check the necessary voltage and power indicated
on the plate near the power cable. The room must be equipped with an earth connection and connections to
the gas and/or vacuum networks, and an air exhaust duct must be installed outside the building.
For the connection to the gas and/or vacuum networks, read carefully chapter 2C.

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00055EN Rev.10 – 12/2011 4
Thimble method example
LEGENDA:
1. Exhaust air from the cabinet.
2. Air from the environment.
3. Bleed Air (100 ÷ 200 m
3
/h more than the exhaust air
flow rate from the cabinet) to a dedicated exhaust
fan (in the electronic board a voltage free contact is
available –see electrical diagram- to check when
ventilation is ON).
For the connection to the gas and/or vacuum networks, read carefully chapter 2C.
The quantity of heat generated by the cabinet, if the air is not extracted ouside the room, is the following for
the eight models respectively:
The quantity of heat generated by the cabinet, if the air is not extracted outside the room, is the following for
the four models respectively:
- CytoFAST Top 209: 410 Kcal/h
- CytoFAST Top 212: 460 Kcal/h
- CytoFAST Top 215: 520 Kcal/h
- CytoFAST Top 218: 540 Kcal/h
The installation must be done by technicians authorized by Faster S.r.l. or by the distributor
Microbiological Safety Cabinet
THIMBLE
3
2
1

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00055EN Rev.10 – 12/2011 5
2.C ELECTRIC/GAS CONNECTIONS and INSTALLATION OF THE WORK SURFACE
The electrical connection of the CytoFAST Top Biohazard cabinet is made by connecting the power cable
located on the upper of the right side of the cabinet to a suitable power point (see technical table) . When the
cabinet is connected, the green light on the control panel switches on (see chapter 4D).
If stipulated by local legislation, insert upstream of the power line an automatic protection overload switch
provided with a differential relay, with a rated switching voltage no greater than 30 mA.
The cabinet can be provided with two gas/vacuum intakes (optional). The connection with the two
gas/vacuum intakes is made according to the type of connection: town gas or industrial gas (air, vacuum,
nitrogen, etc.).
The cabinet must be connected with the town gas mains through an approved conduit for safety reasons.
For the installation of the work surface, proceed as follows:
- remove the protective paper from the work surface resting against the back of the cabinet, taking care
not to scratch its surface,
- remove the padding/sterilization plate,
- open the shatterproof front window,
- clean the work surface with a damp cloth soaked in alcohol or soapy water or with a commonly available
product designed for stainless steel,
- place the table in the work chamber, allowing the back to slide on the chamber's supporting bases up to
its bottom wall,
- close the shatterproof front window.

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00055EN Rev.10 – 12/2011 6
Techincal Features Table
Description Unit CytoFAST Top 209 CytoFAST Top 212 CytoFAST Top 215 CytoFAST Top 218
Overall Dimensions (L x H x P) mm 1015x2290x785 1320x2290x785 1625x2290x785 1930x2290x785
Usefull dimensions (L x H x P) mm 885x660x580 1190x660x580 1495x660x580 1800x660x580
Maximum front aperture mm 420 420 420 420
Working aperture mm 200 200 200 200
Weight Kg 235 270 325 370
Noise level dB (A) <56 <57 <58 <59
Lighting level Lux >1000 >1000 >1000 >1000
Nitrogen, CO
2
, compressed air - maximum pressure bar 4 4 4 4
Fuel gas- maximum pressure mbar 20 20 20 20
Main voltage V 230V AC 2P+T 230V AC 2P+T 230V AC 2P+T 230V AC 2P+T
Frequency Hz 50 50 50 50
Maximum power consumption W 1424 1534 1681 1898
Current A 7.82 8.63 9.18 9.80
Electrical class 1 1 1 1
Protection level IP20 IP20 IP20 IP20
Internal outlet (maximum current for all the sockets: 4A) 2P+T 230V 4A 2P+T 230V 4A 2P+T 230V 4A 2P+T 230V 4A
Fluorescent lamps W 2x30 2x36 2x58 2x58

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3 OPERATION PRINCIPLES
The following are the working principles of the CytoFAST Top cabinet :
- the pressurized air pushed into the plenum of the main motor-fan passes through the absolute filter and
then downwards, in laminar flow, into the working chamber. From here, through the slots of the work
surface, having mixed with the external air which enters the cabinet from the front opening, the air
passes through the absolute filter placed under the work surface and is sucked into the intake up
channel situated at the rear of the working area.
- part of the air (about 30% of the total) is extracted by the second motor-fan through the corresponding
absolute exhaust filter,
- this extracted air generates the intake of air from the outside (front barrier) which ensures the protection
of the operator/environment from hazardous material manipulated in the working area.
- the extracted air may be filtered again through a second absolute exhaust filter placed on the exhaust
duct of the cabinet (BS 5726), if the air is recycled inside the laboratory where the cabinet is installed.

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-
4 OPERATION
4.A SCOPE
The vertical laminar airflow cabinet CytoFAST Top is manufactured in compliance with international
standards for the protection of the material, the operator and the environment against biological (Class II) and
cytotoxic hazards and is suitable for the manipulation of low- and middle-risk pathogenic agents
4.B CONTROL AND REGULATION SYSTEMS
The CytoFAST Top cabinet is provided with an automatic regulation system to keep the airflow speed (0.4
m/sec) in the work chamber and the recycling air/extracted air ratio constant. This system counters the
effects of the gradual clogging of the three HEPA filters up to pressures of nearly 35 mm H
2
O.
The soft-touch control panel is microprocessor-controlled with a display showing all relevant data with regard
to the operating functions, the different alarms and the error messages.
The solid work surface creates optimal airflow laminarity at working level in the preparation of cytotoxic drugs,
while the front longitudinal slots create a protective air-barrier.
The front panel of hardened glass is hinged to facilitate the introduction of bulky objects, and is provided with
gas-springs for opening/closing and holding the "open" position.
The front panel seals the front opening and assures the saturation of the cabinet with sterilizing agents as
well as the non-contamination when not used.

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00055EN Rev.10 – 12/2011 9
4.C REMOTE SIGNALS (OPTIONAL)
The electronic control board can be improved adding the following optional features:
Ventilation status (ON/OFF)
It is possible to obtain a 12 Vdc output to connect a led light or alternatively a Normally Open voltage free
contact to be connected to an external circuit.
There are two different working possibilities:
1 the signal starts when ventilation is turned ON and stops when ventilation is OFF.
2 the signal is flashing during the start up phase of the ventilation and is ON when the correct air flow
conditions are reached.
Correct air flow conditions
It is possible to obtain a 12 Vdc output to connect a led light or alternatively a Normally Open voltage free
contact to be connected to an external circuit. That signal is closed when air flow rate is in the correct range
and it is open in case of any flow alarm.
External consent
On request it is possible to allow ventilation to be turned ON by a remote volt free contact. When the
ventilation is turned ON the cabinet stay in stand by (start up) till the remote normally open contact is closed.
If during normal ventilation the remote contact is open the following message will appear “REMOTE
CONTACT OFF” and the operator has to switch OFF ventilation as soon as possible and then check the
reasons of the failure.