Defiant FROG-5000 User manual

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1
Connecting TheFROGto Ellvin
1. OpentheEllvinGCSoftware
2. Plug serial data cable (Figure5) intodata portonsideofFROG (Figure2).
3. TurnFROG on
4. InEllvinselectthedropdownmenutotherightoftheporticon(Figure6) and
select aCOM port(theFROGisusuallythelastportonthelist).
5. Ifconnectionissuccessfulinthelowerleftcornerofthesoftwareit will display:
CONNECTEDPARAMS LOADED
6. Ifaconnectionwasunsuccessfulit maybenecessarytoinstall theproperdrivers.
ThesecanbeinstalledmanuallybyopeningtheCDMapplicationonthesoftware
USBdriveunder DefiantTechEllvin\USBtoSerial Drivers\CDM 2.04.06.exe.
Important!
DO NOT inverttheinstrumentwiththespargebottle attached.
DO NOT transportor storetheinstrumentwithliquidinthespargebottle.
DO NOT handle or carrysystemwhensample isbeing analyzed.
Powering theFROG
TheFROG mayberun onbatteryor anACpower source.
Battery:
1. ThebatteryisalreadyinstalledintheFROG.
2. Thereisabatteryindicator on thebackoftheFROG.Fivebarsshowthat theFROG
isfullycharged.
ACPower Source:
1. TheFROG canberunonanACPower Source. Torun onpower, plug power supply
(Figure3) intopowersource andtheninto12 VDCPowerportonsideofFROG
(Figure2).
ToturntheFROGon,slidethepowerswitchlocatedonthesideoftheinstrumentinto
theupposition.Agreenlightshouldappear (Figure4).
Installing theEllvinGCSoftware
TheEllvinGCSoftwareislocatedonaUSBDriveinthezipper pouchoftheFROG’s
case.Toinstall thesoftwarerun thesetupfile locatedinthefolderlabeledDefiantTech
Ellvin.
RecommendedComputerSpecifications: Pentiumdual coreor faster, Windows7or
morecurrent, and2GBmemory.
FROG-5000TM Quick Start Guide
Figure2:FROG-5000andcloseupofdataandpowerports
Figure1:Do not invert FROGwith sparge
bottle attached.
Figure4:Powerswitch (onposition)
Figure3:PowerCable
Figure5:SerialData Cable
Figure6:SelectingaCOMporttoconnect FROGtosoftware
Setting up theFROGforSampling
Water/Soil Sampling will require:
SpargeTubeand5mLSyringe.For blankwatersamplesusedistilledor de-ionized
water.
Air Sampling will require:
FROGAirSamplingKit (pg 6).For blank airsamplesthecarbonfiltertubeintheair
samplingkit canbeusedtofilter theair or anyother source ofcleanairmaybeused.
Refertothefollowingpagesfor furtherdetails:WaterAnalysispage4,Soil Analysis
page5, AirAnalysispages6and7.
Beforesampling:
1. Makesureinstrumentsettingsarecorrect for theapplication
2. Calibrate or havetheFrogcalibratedfor theapplication
3. Runablankbeforeattemptingsample analysis.Ablank runshouldproduce aclean
baseline(Figure7)
4. (Optional)AdjustRetentionTimesifusing theFrogat adifferentlocationthanthe
onewhereit wascalibrated. Elevationchangeswill affectretentiontimes. Figure7:CleanbaselineinEllvin,free ofpeaks(Note: there
isaslightpeakinthebeginning,thisisnormalandaccepta-
ble)
Battery
Indicator
Here
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2
SoftwareTabsand Instrument Settings
Settings Description Setting Range
TaHoldtimeat lower GCtemperature(seconds) 2-900 (seconds)
TbRamptimefromGCcoldtoGChottemperature
(seconds) 2-900 (seconds)
TcHoldtimeat hotGCtemperature(seconds) 2-900 (seconds)
CtInitial coldGCtemperature(oC) 1-99 (oC)
HtFinal hotGCtemperature(oC) 30-300 (oC)
COLLECT CollectiontimeofanalytesontoPC(seconds) 10-900 (seconds)
CLEAN TimecleaningPCbyheating(seconds) 2-10 (seconds)
PRESETTLE TimePCcoolsafter cleaning(seconds) 2-20 (seconds)
SETTLE Timeallowedfor pressuretostabilize beforePC
FIRE (seconds) 2-20 (seconds)
FIRE TimePCisheatedtoreleaseanalytes(seconds) 2-10 (seconds)
Changing InstrumentSettings
1. WiththeFrogconnectedtothesoftware,inthesettingstab,locate
thesettingsboxyouliketomodify(Figure11).
2. Intheemptyboxtotherightofthesetting:Enteranon-zero integer
thenclickSETtoapplythesettingstotheinstrument.
Note: Changestothesettingsarestoredontheinstrumentand DONOT
reverttothedefault settingswhenpowerisremovedor computeris
disconnected.
Setting theFrog up foranApplication
ItisimportanttomakesuretheFrogisusing thecorrect settingsfor an
application.IftheFrogwascalibratedbyDefiantor anothercompanythe
settingshavealreadybeenestablishedandset for you.
Thesettingsdefinetemperatureanddurationparametersfor variousphases
oftheinstrument’sanalysiscycle.Thesettingsfor optimal instrument
performance dependonthetarget analyte andshouldbeset accordingly.
Figure9diagramsthesettingsdescribedinFigure10.
Software Tabs
IntheEllvinGCSoftwaretherearefourdifferentwindowsthat are
accessedwithtabsat thetop leftofthescreen(Figure8).Eachtabisused
for thefollowing:
• LiveData: liveviewingofaFrogsample run withtheFrogconnected
tothesoftware
• AnalysisTab:analysisofcompletedinstrumentruns
• CalibrationTab:creating, loading or viewingacalibrationfile
• SettingsTab:viewing or modifyinginstrumentsettings. Figure8:TabsinSoftware
Figure9:DiagramofFROGInstrument Settings.
Figure11:Table ofcurrent instrument
settings, withadjustmentboxes.
Figure10:SettingsDescriptions
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3
Calibration
Calibration
It isnecessarytocalibratetheFROGforadesiredapplication.Acalibrationmustbe
openedintheEllvinSoftwarefortheFROGtocorrectlyanalyze samples.
Calibrationfiles canbecreated(withanalysisdatafromchemicalstandards)or
loaded(ifsomeoneelse calibratedtheFROG)withthesoftware.Defiant
recommendsre-calibratingtheFROGatleasteverythree weeksforthemost
accurateanalyses.
Loading anExisting CalibrationFile
IftheFROGwas calibratedbysomeoneelse,thecalibration filewill mustbeloaded
alongwiththesoftware.ThecalibrationfilecanbefoundontheUSBDrivelocated
inthezipperpouchintheFROG’scase.Toloadacalibration filecompletethefol-
lowingsteps:
1. LocatethefileontheUSBDrive.Itisafileendingin.ana.Copyand pastethe
filetoalocationonthecomputerthatiseasytofind.
2. InthecalibrationtaboftheEllvinGCSoftwareclickon theopenicon tothe
rightofthe“analytefile”box,locatethefilethatwas copiedandpastedand
openit (Figure12).
3. Verifythatthetargetanalytes arelistedundertheanalytes list(Figure13).
4. IftheFROGwas calibratedatadifferentelevation thantheoneitwill beused,
adjustretentiontimes.
Creating a CalibrationFile
Tocreateacalibration filedo thefollowing:
1. Createanewcalibrationfilebyclickingonthe“new”icon totherightofthe
calibrationfilebox(Figure14).
2. Labeland saveyournewcalibration file.
3. Add youranalytesbyclickingon the“new”icon underneaththeanalytelist
(Figure13).Typeintheanalytenameand alias (whichwill appearon thedis-
playwhenrunninginstandalonemode)and selectOK.Repeatthisstepfor
eachtargetanalyteuntil all desiredanalytes havebeenadded.
Generating and AddingData to a Calibration
Tocreateacalibration,aseries ofdifferentknownconcentrationsamples mustbe
run ontheFROG.Defiantrecommendsrunningfivecalibrationstandardsfrom
lowesttohighestconcentration.Refertolatersectionsregardingrunningsamples
and analyzingthedata.Thestepstogenerateand adddatatoacalibrationareas
follows:
1. Run5knownsamples ontheFROGstartingwiththelowestconcentrationsand
endingwiththehighestconcentration,notingwhichconcentrationcorresponds
toeachrun.
2. Analyze oneofthesamplerunsintheanalyzetabofthesoftware(see page9
forhowtoanalyze data).
3. Intheresultstableundertheanalyze tab,highlighttheanalyteyouwouldlike
toaddcalibration dataforand enterintheknownconcentration
4. Withthedesiredanalyteselectedintheresultslist, rightclick on thehighlight-
edrowandselectthecorrectanalytefromthelist.Selectyes whenthesoftware
asksifyou wanttocopythepeakstothecurrentcalibration,thissendsthedata
tothecalibration file.Repeatsteps3and 4forall analytes ateachconcentra-
tionrun.
CalibrationCurveand Equations
Once datahas beenaddedtoacalibration,Ellvinwill createacalibrationcurvefor
thetargetanalyte(s).Figureshowsanexamplecurve.Thecalibration curvedisplays
thedegree ofcorrelation (R²value)betweenanalytestandardsofvaryingconcentra-
tionsandtheinstrument’sresponse tothem.Thecorrelation iscalculatedas botha
linearequationandasecond-orderquadraticequation.Theusermayviewtheequa-
tionsandchart foreitherPeakArea correlation orforPeakHeightcorrelation.Ellvin
uses these parameterstocalculateconcentrationsforfuturesamples.Notethatfor
samplescreening,aonepointcalibrationmaybeused,thispointshouldbethesame
concentrationoftheaction levelforthescreening.
Figure12:Openinga CalibrationFile.
Figure14:Creatinga NewCalibrationFile
Figure15:Addinga NewAnalytetoa CalibrationFile
Figure13:AnalytesList and
addingnewanalytes.
Figure16:SendingDatatoa CalibrationFile
Figure17:CorrelationEquationsandCalibrationCurve
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4
WaterSampling
Water SamplePreparationTips
• Thesample volumefor theFROG willalwaysbe
5mL.Thesystemwascalibrationwith5mL samples, all
testing mustbeperformedusing 5mL samples.
• Itisacceptable for watersamplestohaveparticulate
matteror sedimentinthem.Thiswill notdamagethe
FROG.
• Ifthewater issilty,it isbesttoloadthesample directly
intothespargebottle.
• Bemindfuloftheconcentrationofthesample.Ifthe
sample smells, it isagood ideatodilute it.
• Donot run neat(undiluted) materialslikecrudeoilor
solventswiththeFROG.
Loading a Water Sample
1. Load5mL ofthewatersample intothe5mL glass
syringethat comeswiththeFROG or intoaplastic
5mL syringe.Thiscanbedonebyeitherpulling the
liquidupthroughthefrontofthesyringeor by
removing theplungerandfillingthesyringethrough
theback.
2. Threadthegreenspargeneedle intothespargebottle,
leavethebottle inthedropped(down)position(Figure
18).
3. Connect Syringetosampleinlet ontheinstrument, itis
aluer-lockconnectionandthesyringewill stayin
place (Figures19 and20).
4. Set valveontop ofinstrumenttothe“loadwater”
positionsotheredarrowpointstoward theconnected
syringe(Figure21).
5. Inject sample intotheinstrument; thespargebottle
nowholdsthesample (Figure22).
6. Liftthespargebottle upintotheraised(up)position.
Tightenspargenut(counterclockwise)toholdbottle
inplace (Figure23).
7. Set thevalveontop ofinstrumenttothe“run water”
position.Theredarrowwill nowpointtoward theblue
tubing(Figure24).
8. Removeemptysyringefrominstrument.
Step 2
Running a Sample
Once awater sample hasbeenloaded, it isreadytoberun
withtheFrog.
1. WiththeFROG connectedtothesoftwareusetheplay
buttontostartarun(Figure25).
2. WhentheFrogisfinishedrunningthesample,empty
thespargebottle,cleanglasswarebyrinsing3times
withwater, runablankiftherewasadetectionand
thenproceedwithsampling.
Figure18:Sparge
Bottle inDropped
(Down)Position
Figure19:Attach-
ingandDetaching
SyringefromSam-
ple Inlet
Figure20:Sparge
Bottle inDropped
(Down)Position
with 5mLSyringe
attachedtoFROG,
Sample isreadyto
beloaded
Figure21:Valve
set to“LoadWa-
ter”
Figure22:Sample
InjectedinSparge
Bottle
Figure23:LiftingSpargeBottle upand
tighteningspargenut toholdit inplace Figure24:ChangingValve position
to“RunWater”
Steps3,8
Step 3
Step 4 Step 5
Step 6 Step 7
Figure25:PlayButton
tostartrun inSoftware
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Soil SampleTips
• Soil samplingissimilar towater sampling.
• Soil canberundirectlyinthespargebottle.For this
method,themass ofthesoilrun will alwaysbe1g;
5mL ofwater isthenaddedtothesoil prior torunning.
• Keeptrackofanydilutionperformed.
• MethanolExtractioncanbeperformedonthesoilif
highlycontaminated.
• Rinsebetweensoil samplesandmakesuresparge
needle iskeptclean.
Loading a Soil Sample
1. Weigh out1g ofsoil,pourintospargebottle (Figures
26 and27).
2. Fill 5mL glass syringewith5mL ofcleanwaterand
dispensedirectlyintospargebottle,rinsingdownany
soil that maybestucktothesidesofthebottle (Figure
28).
3. Liftspargebottle withsoil andwaterintotheraised
(up) positionontheFROGandtightenspargenut
(counter clockwise) toholdbottle inplace (Figure29).
4. Set thevalveontop of instrumenttothe“runwater”
position.Theredarrowwillnowpointtoward theblue
tubing(Figure30).
Running a Sample
Once asoil sample hasbeenloaded,itisreadytobe
analyzedwiththeFROG.
1. WiththeFROG connectedtothesoftwareusetheplay
buttontostartarun(Figure31).
2. Makenote ofthedilutionofthesample.If1gofsoil
wasdilutedin5mL ofwater, thisgivesadilution
factor of5,soanyconcentrationresultswill needto
bemultipliedby5toobtainthetrueconcentration of
analyte inthesoil.
3. WhentheFrogisfinishedrunningthesample,empty
thespargebottle,andcleanglasswarebyrinsing 3
timeswithwater. Makesureall soil isremovedfrom
thespargebottle.Ifthereissoil stucktothesparge
needle,acleandampKimwipemaybeusedtowipeit
off.
4. Runablankiftherewasadetectionandthenproceed
withsampling.
Figure31:PlayButton
tostartrun inSoftware
MethanolExtraction
MethanolExtractionmaybeperformedonhighly
contaminatedsoils.Ifthesoil hasastrongodor,it isa
good idea toperformmethanolextractiononit.Methanol
Extract isdilutedtoa5mL sample volumeandthenrun
thesamewayawatersampleis.For detailsonMethanol
Extractionpleasesee theapplicationnote at:
www.defiant-tech.com.
Figure30:Valveset to
“RunWater”Position
Figure29:Lifting SpargeBottle intotheRaised(Up)
PositionontheFrog
Figure27:Pouring
Soil intoSpargeBot-
tle
Figure28:RinsingSoil
DownSpargeBottle
with5mL water.
Figure26:1gofsoil
weighedout.
Step 1
Step1 Step2
Step3
Step4
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SoilSampling

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6
AirSampling
AirSampleTips
• Makesureferrulesandairsamplerare
installedcorrectly
• Avoidpullingparticulateswiththeair
sampler. Filtersareavailable for air
samplinginhigh particulate matter
environments
Installing theAirSampler
1. Slidegreenspargeneedle intoopening on
top ofairsampler. Liftairsamplerupand
securebytighteningspargenut(counter
clockwise) aroundair sampler(Figure33).
2. Install couplerline;thewhite fittingwill
attachunderneaththeairsamplerlabel on
theFROG (Figure34).
3. Plug inpowercable,theportisin
underneaththeairsamplerlabel onthe
FROG (Figure35).
4. Makesurethevalveonthetop oftheFROG
isset to“Run Air”(Figure36)
5. Connect endoftransferlineto:
-TedlarBagadapter(forsamplingaTedlar
Bag)
-CarbonScrubber(for providingablankair
sample)
-AirSamplerWand(for collectinganair
sample)
Note that thetransferlinedoesnotneed
anythingattachedtoit ifsamplingambient
air. See nextpagefor moredetail on
samplingmethods.
Air
Sampler
TransferLine
Coupler
Line
Power
Cable
TedlarBag
Adapter
Carbon
Scrubber
Figure32:Air Sampler Kit.
Air
Sampler
Wand
Figure33:InstallingAir
Sampler onFROG Figure34:Connecting
Coupler LinetoFROG
Figure35:PlugginginAir
Sampler Power Cable
Figure36:Valveset to“RunAir” Figure37:FROG Readyfor Air Sampling
Running anAirSample
Once air samplerhasbeeninstalled:
1. WiththeFROG connectedtothesoftware
usetheplaybuttontostartarun (Figure38).
2. TheFROG will pull anairsample for the
firstsixtysecondsoftherun,thenit will
analyze thesample.
3. TheFROG canalsobeset for continuousair
sampling.Todothis,holddowntheshift
keywhile clickingontheplaybutton.Select
thetypeofcontinuoussampling youwould
like.Thissequence willrun until youpause
theunit byclickingonthestopbuttoninthe
Ellvinsoftware.
Step 1 Step 2 Step 3
Figure38:PlayButton
tostartrun inSoftware Step 4
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AirSamplingMethods
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AirSampling Methods
Thereareseveral differentoptionsfor air
samplingandhowtotakeairsamples.Note that
fingertightconnectionsareadequate (and
recommended) for all ofthese methods.
Direct Sampling
1. Noattachmentisneededfor thetransfer line
2. Theairsample will bepulledinthrough the
endofthetransfer line(Figure39).
CleanAir (Blank)Sampling. Runablank
sample beforestartinganalysisandinbetween
samples.
1. Screwthecarbonscrubberontotheair
sampler transfer line(Figures40 and41).
TedlarBag Sampling.
1. Twistsilverendofadapterontothetransfer
line(Figure42).
2. LoosenBlackpartofadapterfromsilver
part(oneturnisadequate). Slideblackpart
ofadapterontothestemofthetedlarbag
(Figure43).
3. Twistblackandsilverpartsofadapterto
tightentheadapteraroundthetedlarbag
stem(Figures43 and44).
4. Makesuretedlarbagvalveisintheopen
positionprior tosampling.
Air Sampler Wand. Thiswandisdesignedfor
samplinginsideasmallspace,for example
samplinginsideacabinet or drum.
1. Twistsilverthreadedendofairsampler
wand(Figures45 and46).
2. Ifusing inadustyenvironment,usea
particulate filterandadapter. Thiskeeps
particulatesfromdamaging theair sampler.
Figure39:Air Sampler Trans-
fer Line, noattachments, ready
for direct sampling
Figure40:Attaching Scrubber toTrans-
fer Line Figure41:Scrubber AttachedtoTransfer
Line, readyfor cleanair (blank) sampling
Figure42:Attaching Silver Fittingon
Tedlar BagAdapter toTransfer Line Figure43:ConnectingAdapter toTedlarBag
Figure44:Tedlar Bagconnectedto
Transfer Linevia Adapter, readyfor
sampling.
Figure45:Attaching threaded
fittingonWandtoTransfer Line Figure46:WandattachedtoTransfer Line,
readyfor sampling.
Figure47:Sampling withtheAir Sampler
Wand.
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8
RunningSamples:LiveDataTab
ChromatogramFeatures(Figure 55)
• Theredlineisthedata fromthePID.Thepeaksonthislinerepresent
differentcompounds.
• Thebottom(x)axisrepresentstime(inseconds). Theleft(y)axis
representsclicksfromthe PID,andtherightaxisisfor GCtemperature(°
C).
• Thepurple linerepresentsGCtemperature.
UsefulTools/FeaturesinLive Data
• The MagnifyingGlass (Figure53) maybeusedtozoomintoa
chromatogram. Touse:
1. Clickanddragmouseover areatomagnify.
2. Releasethemousebutton.
3. Tozoomout, double clickonthemagnifyingglass
• The GCTemperature Indicator (Figure54) displaystheGC
temperature.Theiconis GREEN wheninstrumentisreadyfor analysis
and RED whentheGCiscooling
Running a SampleintheLiveData Tab
1. AftersettingFROGuptosample,makesurethelivedatatabisselected
intheEllvinGCsoftware(Figure48).
2. Connect FROG tosoftwareusing theportdrop downmenu(Figure49).
3. Tostartasample, usethelargeplaybuttoninthetop toolbar (Figure50).
4. Beneaththechromatogrammakeanynotesaboutthesample.The
softwarewill savethesenoteswiththechromatogram(Figure51).
5. AfterFROG isfinishedanalyzing,thesoftwareautomaticallysavesthe
data.Afile locationwiththelognumberwill bedisplayedat thetopof
thechromatogram(Figure52).
6. Tostartanewsample,followtheproceduresfor loadingasample then
repeat steps1-3, makingsuretoupdate thenotes.
Figure49:SelectingaCOMporttoconnect FROG tosoftware
Figure50:PlayButton
tostartrun inSoftware
Figure48:LiveData TabinEllvinSoftware
Figure51:MakingNotes
for asample run
Figure52:SavedFile Location, note thatdata (andanyasso-
ciatednotes) areautomaticallysavedat theendofeachrun.
Figure54:GC Temperature
Figure53:MagnifyingGlass Tool
Figure55:ChromatograminLiveData TabwithFeatureslabeled
Start/Stop Button
PortSelection
MagnifyingGlassTool File SavedLocation
Bottom(x) axis:timeinseconds
Left(y) axis:Sensor Data (clicksfromPID)
Rightaxis:GCTemperature
ConnectionIndicator GCTemperatureIndicator
NotesSection
GCTemperatureData
ChromatogramPIDData
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GettingResults:Analyze Tab
Opening a FileintheAnalyze Tab
1. Makesuretheanalyze tabisselectedintheEllvinGCsoftware(Figure
56).
2. Clickontheopenicon(Figure57 )inthetop toolbarandnavigate tothe
Ellvindata folder, thedefault filepathis:
C:\Users\Username\Documents\EllvinData
3. Locate thefile youwishtoopen.Filesaresavedinfoldersbydate and
theneachfile hasitsowntimestampandlog number. Clickingonce on
thefile will showapreviewofthechromatogram(Figure58).Clickon
“select” toopenthefile.
4. Theselectedchromatogramshouldnowappearintheanalyze tabandis
readyfor analysis(Figure59).
Analyzing a File
1. First,makesurethecorrect calibrationisloadedinthecalibrationtab.
Refertopage4ofthequickstartguidefor detailsaboutloadinga
calibration.Ifacalibrationisloaded,vertical blueanalyte windowswill
bepresentonthechromatogram.
2. Lookfor peakswithintheblueanalyte windows.Ifthereisapeakwithin
awindowit meansthat thereisadetection.Note thepeaksintheblue
windowsinFigure59.
3. Integrate thechromatogram.Thereareafewmethodsfor doing this;see
sectionbelow.
4. Locate theresultstable belowthechromatogram(Figure63).
Concentrationsfor eachcalibratedanalyte peakwill belisted.
Howto Integrate:
Thegoal ofintegrationistodeterminethepeakarea underneaththecurveon
thechromatogram.
MethodA:AutoAnalyze.ClickontheAutoAnalyzebutton.Thiswill
integrate all thepeakspresentonthechromatogram.Thedata will appearin
theresultstable.
MethodB:ManualIntegration. TherearetwoIntegrationToolsthat maybe
usedfor manual integration.Integrator 2isalmostalwaysthebestchoice for
integration.
Integrator1
The user createsabaselineandintegratespeaks,thencalculatesthepeak
heightandarea todetermineanalyte concentrations.Beginningandending
pointsoftheintegrationarebasedonthecursor position.
Integrator2
WithIntegrator 2,Ellvinsnapstothevertical pointonthedata lineclosestto
thecursor position. Integrator 2isrecommendedfor easeofuse.
ToManuallyIntegrate:
1. Basedonretentiontimes, determinewhichpeakstointegrate.
2. Clickandholdthemouseatthevalleybeforethefirstpeaktointegrate (In
Figure62:Valley1).
3. Dragthemousetothevalleyafterthepeaktointegrate(InFigure62:
Valley2).Donotdragthelinethrough thepeaks.Ahighlightedarea
displaystheintegratedpeaks. Multiple peaksmaybeintegratedat thesame
time.
4. Whentheintegrationiscomplete, thedata will appear intheresultstable.
Exporting Data fromEllvin
Theeasiestwaytoexportdata fromEllvinistousetheviewreporttool.
1. ClickonViewReport(Figure64).Ellvinwillgenerate areportwiththe
chromatogramandresultstable.
2. Once thereportisgeneratedit canbesavedasapdf,word documentor
excel file (Figure65).
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Valley2Valley1
Figure57:OpenIcon
Figure58:OpeningaChromatogramFile
Figure59:ChromatogramFileOpenedinAnalyze Tab
Figure60:AutoAnalyze Figure61:ManualIntegration Tools
Figure56:Analyze TabinEllvinSoftware
Figure62:ChromatogramwithValleyslabeledfor manual
integration
Figure63:ChromatogramIntegrated, Data showninResults
Table.
Figure65:ReportwithSaveIconCircled.
Figure64:ViewReport

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10
Frog-5000™PackingInstructions:ItemList
1. Syringe
2. SpargeBottle
3. Frog (With SpargeTube Protector)
4. Serial DataCable
5. Battery
6. AirSampler
a.AirSamplerCouplerLine
b.TransferLine
c. 1/16-3/16Adapter
d.Activated CarbonScrubber
e.AirSamplerWand
7. PowerSupply
8. PowerCord
9. 60 mLRinsingSyringe
10. SoftwareonUSB drive
11. MicroSDtoUSB Adapter
NOTE: The batteryshouldbe left
inside theunitforground and aircargo
shipping.
Forpassengeraircargo,the battery
shouldbe removed,safely packed and
carried onboard.Inthis case,the
batteryshouldhaveless thana30%
charge.
Ifthe batteryis shipped outside ofa
unititshouldhavelessthana30%
chargebeforetransport.
1 2
4 5
6
a
8
3
b
c
d
e
7 9
11
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Frog-5000™PackingInstructions:ItemLocations
3
1. Syringe
2. SpargeBottle
3. Frog (With SpargeTube Protector)
4. Serial DataCable
5. Battery(InFrog)
6. AirSampler
a.AirSamplerCouplerLine
b.TransferLine
c. 1/16-3/16Adapter
d.Activated CarbonScrubber
e.AirSamplerWand
7. PowerSupply
8. PowerCord
9. 60 mLRinsingSyringe
10.SoftwareonUSB drive
11.MicroSDtoUSB Adapter
1 2
4
3
5
6abcd
7
8
9
(10 and 11inzipperpouch attached tothetopofthe case)
6e