Drawell DW-2002H User manual

EPI-FLUORESCENT
MICROSCOPE
SERVICE MANUAL
Read it carefully before operating and keep it with the microscope

Attention
Dear Sir or Madam:
Thank you for using Epi-fluorescent microscopes. As one of the professional designers,
manufacturers and distributors for optical instruments in China, since 1978, we have been working
for supplying the new or old customers worldwide with high-quality and low-cost products. We
wish that our products could bring you success and satisfaction. We enjoy offering you the most
suitable products and the best service.
This manual gives a minute description of the structure, principle, configuration operating
guide, troubleshooting, maintenance and some attention for Epi-fluorescent microscopes. Please
read it carefully before you use, and keep it for long time.
In particular, the following notes must be understood thoroughly and obeyed strictly:
1. Permitted use:
Being high-precision laboratory apparatus, it is combined perfectly by an
Epi-fluorescence device and an ordinary microscope. It is not only used for fluorescent
microscopy observation, but also used for general biological microscopy observation.
2. No dismantle the equipment:
Unless you are a microscopic expert, or there is a special guide about doing so in the
manual, please don’t dismantle your microscope. Otherwise, it will damage the microscope
seriously, and reduce greatly its accuracy and using-life. When you identify some troubles,
and can’t troubleshoot them by yourself according the manual, please contact us or our
representative in you area.
3. Safety:
----The lamp-house of the Epi-fluorescence device and the base near the illuminator will
be very hot to burn your fingers. Don’t worry about it, but it must be treated carefully.
Please take care of yourself and take the combustible material (such as gasoline, paper,
plastic and cloth) far away from the microscope.
----Before change the mercury lamp in lamp-house or the halogen lamp in base, ensure
that it has been disconnected with the power source. The new bulb must be the same
specifications as the old one. At same time, ensure the lamp cool enough; otherwise the hot

bulb will burn your fingers.
----After fluorescent microscopy observation is over, the first turn off the switch of the
power supply box; the second disconnect the box with the power source; the third disconnect
the lamp house with the box. Ensure to not unplug the connecting line from the power supply
box before its switch is turned off.
----The mercury lamp should be restarted ten minutes later after it is turned off.
4. Use the correct power supply voltage:
The power supply voltage must be fitted to the microscope; otherwise it will damage
the circuit and bulb, even lead to insecurity.
5. Protecting optical parts:
Never try to contact directly the optical surface of objectives, eyepieces and other
optical parts with your finger. Fingerprints will seriously affect your observation results.
6. Don’t leave any dust and fingerprints on the bulb, otherwise it may affect its life and
illuminating efficiency.
7. Working surroundings requirements:
Room temperature: 0°C-40°C; The highest relative humidity: 85%
High temperature and humidity can cause mildew and damage the instrument.
8. Microscope is a precision instrument, soft and gentle operation is necessary. Any rude
action or hard shake may damage it.

CONTENT
A) Structure of the Epi-fluorescence Microscope ……………P.1
B) How to Assemble the Epi-fluorescence Microscope………P.2
C) The Principle, Function and Main Technical Parameters of the Fluorescence
Microscope …………………………..........................………P.3
D) How to Use the Fluorescence Microscope …………………P.4
E) Use Scope of the Fluorescent Filter …………………………P.5
F) How to Shoot the Troubles in the Fluorescence Microscope
………………………………..………………P.6
G) Maintenance and Care of Microscope ………………………P.6

A) Structure of the Epi-fluorescence Microscope:
1. Flashboard A 2. Eyepiece
3. Viewing Head 4. Lock Screw for Head
5. Excitation Type Selector 6. Flashboard B
7. Door Screw for Lamp House 8. Adjusting Screw (up/down)
9. Adjusting Screw (left/right) 10. Lamp House
11. Lock Screw for Fluorescent Device 12. Lock Screw for Ultraviolet Bar
13. Fluorescent Objective 14. Microscope Body
15. Brightness Adjustor 16. Tension Guide Line
17. Switch 18. Accumulator
19. Power Supply Box 20. Ultraviolet Bar
21. Observation Window 22. Epi-fluorescence Device
Fig.1. Structure of the Epi-fluorescence Microscope:
B) How to Assemble the Epi-fluorescence Microscope:
The Epi-fluorescence Microscope is separated into several parts for transiting safety, as the
following:
1）Microscope Body（14）；
2）Viewing Head（3）；
P.1
2
6
8
9
11
20
1
12
13
14
3
4
5
7
15
19
18
17
16
21
10
22

3）Power Supply Box（19）；
4）Epi-fluorescence Device（22）；
5）Eyepiece（2）；
6）Objective（13）；
7）Mercury Lamp；
8）Ultraviolet Bar（20）；
9）Other Parts。
Install all necessary parts together as Fig.1 before using.
1. Unpack the microscope and its parts carefully, check and sort out all parts according to the
packing list。
2. Loose the screw(7) in the Epi-fluorescence device, and open the door of the lamp house。
Remove the nylon rod, and instead of mercury lamp. Caution: Ensure the mercury lamp in
correct position. Finally, shut the door, and fix the screw (7).
Caution: Don’t leave any dust and fingerprints on the mercury lamp to affect its life and
illuminating efficiency. Clean it with soft cloth and gasoline promptly.
Remove the plastic cover away from the Epi-fluorescence device (22), and install it on the
microscope body (14) with the screw (11)。
Usually, the lamp house in the Epi-fluorescence device (22) is vertical, showed in Fig.1. To suit
for some microscope body sometimes, it is necessary to readjust the lamp house horizontal by
yourself, showed as Fig.2. It is very easily: Loose 4 screws (23) remove the insulated pipe (24) to
the left and rotate it to find 3 screws through the cooling holes (25) and loose them. Then turn the
lamp house 90°and fix it with 3 screws. Finally replace the insulated pipe (24) and fix it with the
screws (23). Showed in Fig. 2 and Fig. 3
23. Screws to Fix the Insulated Pipe 24. Insulated Pipe
25. Cooling Holes 26. Focusing Rod for Condenser
Fig.2 Epi-fluorescence Device（Lamp house is turned 90°）
P.2
23
24
25
26
6

Fig.3 Epi-fluorescence Microscope（Lamp house is horizontal）
3. Install the ultraviolet bar
（20）
in the Epi-fluorescence device with the screw (12) and gaskets.
4. Plug the power cord of the Epi-fluorescence device into the socket (OUT) of the power
supply box (19) and fix it.
5. If the objectives are packed in plastic bottles, please take them out and spin them in the
nosepiece.
6. Install the viewing head (3) on the Epi-fluorescence device (22) with the screw (4).
7. Insert the eyepieces (2) into the tubes of the viewing head (3).
8. Connect the power supply box (19) with the power source.
C) The Principle, Function and Main Technical Parameters of the Fluorescence
Microscope:
P.3
4

1. The Principle:
The light source of the Epi-fluorescence device will eradiate light with specific wavelengths.
It will be transmitted on the sample and excite the sample to produce fluorescence. You may have a
high brightness and high contrast image to achieve fluorescence observation and analyze.
2. Function:
The range of the spectrum excited: 350nm ～580nm
The range of the fluorescence spectrum: 420nm ～650nm
3. Technical Parameters and Outfit:
Name
Specification
Quantity
Epi-fluorescence
device
B（blue）
EX490；DM510；BA515
1Set
G（green）
EX545；DM580；BA590
O（ordinary transmission light）
Microscope body
Coaxial coarse and fine focusing mechanism; Double
layers mechanical stage; ABBE condenser; Built –in
halogen lamp with brightness adjustor. Koehler
illuminating system.
1Set
Viewing head
Articulated free binocular head; or Articulated free
trinocular head; or sliding binocular head; or sliding
trinocular head.
1Set
Eyepiece
Wide field plane-scope eyepiece :
WF10X/18；WF16X/13
1Pair/each
Objective
Achromatic objective for fluorescence device：
4X；10X；40X(s)；100X(s, oil)
1Set
Power supply box
In: AC90-254V；Out: 100W DC；Fuse: 5A
1Set
Light source
Mercury lamp
HBO Ultra-high pressure mercury
spherical lamp: 100W
2Pcs
Halogen lamp
6V/20W Halogen lamp; Fuse: 1A
2Pcs
Power cord
1Pcs
Immersion oil
Special immersion oil for fluorescence device
1bottle
Ultraviolet bar
1Pcs
Service manual
1Copy
D) How to Use the Fluorescence Microscope:
1. Turn on the switch (17) of the power supply box (19). It will be in full working condition
after 10 minutes. The tension-guide line (16) will show the state of the mercury lamp.
2. Adjusting the center of the mercury lamp：
Push the excitation type selector (5) to the position “o”, and push the flashboard B (6) in a
middle position. You will find a light shadow on the observation window (21). If the light shadow
is not in the middle of the window, rotate the screw (8) to move the shadow up/down, rotate the
screw (9) left/right.
3. Adjusting the condenser of the Epi-fluorescence device：
P.4

Thrust the excitation type selector (5) into the limiting position “B”. And place a white paper
on the stage. Turn the 10X objective in working. Then turn on the switch of the power supply box
(19) and a light shadow will fall down on the paper. Adjust the focusing rod for condenser (26) and
the focusing knob of the microscope to focus the shadow to be a dot.
4. Preparation of the sample:
The sample can not be too thick. Otherwise the judgment will be affected by the overlapping
cells or impurity.
Thickness of cover glass: 0.17mm;
Seal carrier: no fluorescence and no color;
Immersion oil: Special immersion oil for fluorescence device.
5. Fluorescent microscopy observation:
Turn off the halogen in the base and turn on the mercury lamp.
For the best fluorescence observation effect, it is necessary to close the field diaphragm in
the base or cover the collector with pressboard. Because the lenses in collector possibly
reflect ambient light to disturbance the fluorescent observation.
Push the flashboard B (6) in the middle position to pass the light with specific wavelengths.
According to the different demands, select the different spectrum range:
Pull the selector (5) out to the limiting position for G (green light) exciting state;
Push the selector (5) into the limiting position for B (blue light) exciting state;
Put the selector (5) in the middle position for general biological microscopy observation with
ordinary transmission light;
When the fluorescent microscopy observation is paused, please push the flashboard B (6) to the
left limiting position to stop the stimulating light and reduce the fluorescence quenching.
6. General biological microscopy observation:
Turn off the switch (17) of the power supply box (19) and push the selector (5) to the middle
position “O”. Turn on the switch in the base and adjust the brightness of the halogen lamp by
turning the plate (15) for general biological microscopy observation.
E) Use Scope of the Fluorescent Filter:
Filter
Use
Fluorescent Dye
Lighting
Color
Medicine Examination
Project
B
（Blue）
EX: 490
DM:
510
BA: 515
P1TC；
Pyoktanin Yellow O；
Alkalinity
Fluorescein；
Acridine Orange；
Fluorescein Sodium;
Tetracyclin Sodium;
Green,
Yellow
Orange;
Red
Fluorescence Immune
Body;
Cell Nucleus of the
Tubercidin Bacillus；
Cancer Cell、Red Blood Cell
Protozoan
Venereal Diseases Series
G
（Green）
EX: 545
DM:
580
BA: 590
Luo Daming;
Luo Daming B200;
Acridine Orange
Orange-red
Fluorescence immune body
*DNA
P.5

F) How to Shoot the Troubles in the Fluorescence Microscope:
If there are some troubles in operating, please recheck the instrument carefully as the
following describing before connect with us or our representative in your area.
1. Troubles in electric system:
Troubles
Cause
Remedies
The power supply
box can not be
started
Power cord is not be connected
Connect the box wit the power source
correctly
Fuse is broken
Change it.
The mercury can
not work
The mercury lamp is not
electrified
Connect the lamp house with the
power supply box correctly.
The mercury lamp is broken
Change it
2. Troubles in optical system：
Troubles
Cause
Remedies
Nothing or too
dark in the field
after turn on the
mercury lamp.
Flashboard B (6) is not in its position
Push the flashboard B (6) in
correct position
the selector (5) is not in its position
Push the selector (5) in correct
position
The filter system is inconsequence
Select appropriate lens
The image is
blurring and low
contrast
Objective or filter is dirty
Clean it
The filter system is inconsequence
Select appropriate lens
Immersion oil produces the fluorescence
Use special immersion oil
The lamp in base is not turned off
Turn off the lamp
The lenses in collector reflect ambient light
to disturbance the fluorescent observation
Close the field diaphragm in
base or cover the collector
with pressboard
The brightness of
the image is not
even
The nosepiece is not in its position
Turn the nosepiece in correct
position
Flashboard B (6) is not in its position
Push the flashboard B (6) in
correct position
Epi-beam is not in the optical path
Correct it.
The mercury lamp is not centered, or the
condenser is not in focus
Readjust it
G) Maintenance and Care of Microscope:
1. Unpack the microscope carefully to prevent the accessories such as lens from falling down
and damaging.
2. All lens are calibrated, don’t try to dismantle then apart by yourself.
3. Nosepiece and focusing system are advanced and precise in construction. Don’t try to
dismantle them apart by yourself. Please connect with an authorized technician when they are in
trouble.
4. Keep the mechanical parts from dust, and add a few no-corrosiveness lubricating grease into
the sliding sections at regular intervals. Keep the optical elements clean when
P.6

wipe the microscope.
5. Keep the microscope in dry and cool place. Disconnect it with the power source and put the
dust cover over it after using. If it will be not used for a longer time, it is the best way to screw the
objectives out and place them into the lens-bottles, and screw the dust covers on the nosepiece.
P.7