Leica S Series User manual

Living up to Life
Leica S series
User Manual

Leica S Series User Manual 2
General Instructions
Safety concept
Before using your stereomicroscope for the first
time, please read the "Safety concept" brochure
included with your instrument. It contains
additional information about handling and
care.
Use in clean rooms
The Leica S series can be used in clean rooms
without any problems.
About cleaning
ODo not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
ONever use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces, and
specimens could be contaminated by abra-
ded particles.
OIn most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accesso-
ries for use in clean rooms.
Servicing
ORepairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
OEnsure that the Leica stereomicroscope
is operated, maintained and repaired by
authorized and trained personnel only.

Leica S Series User Manual 3
Important Safety Notes
User Manual
The individual modules of the Leica stereomi-
croscope include an interactive CD-ROM with
all relevant user manuals in several languages.
Keep it in a safe place, and readily accessible
to the user. User manuals and updates are also
available for you to download and print from
our web site www.leica-microsystems.com.
This User Manual describes the special func-
tions of the Leica StereoZoom® stereomicro-
scopes (S series) and contains important inst-
ructions for their operational safety,
maintenance, and accessories.
The "Safety Concept" booklet contains additi-
onal safety information regarding the service
work, requirements and the handling of the
stereomicroscope, accessories and electrical
accessories as well as general safety instruc-
tions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instru-
ments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.

Leica S Series User Manual 4
Symbols Used
Warning of a danger
This symbol indicates especially impor- tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
OHazards to personnel
OFunctional disturbances or damaged inst-
ruments
Warning of hazardous electrical voltage
This symbol indicates especially impor- tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
OHazards to personnel
OFunctional disturbances or damaged inst-
ruments
Danger due to hot surface.
This symbol warns against touching hot surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional infor- mation or explanations that intend to
provide clarity.
Explanatory notes
OThis symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.

Leica S Series User Manual 5
Safety Instructions
Description
The individual modules fulfill the highest requi-
rements for observation and documentation of
Leica stereomicroscopes of the S series.
Intended use
ORefer to "Safety Concept" booklet
Non-intended use
ORefer to "Safety Concept" booklet
Never use S series microscopes or their compo-
nents for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
The devices and accessories described in this
User Manual have been tested with regard to
potential hazards. The responsible Leica affiliate
must be consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Place of use
ORefer to "Safety Concept" booklet
OElectrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
OAvoid large temperature fluctuations,
direct sunlight and vibrations. These condi-
tions can distort measurements and micro-
graphic images.
OIn warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Responsibilities of person in charge of
instrument
ORefer to "Safety Concept" booklet
Ensure that:
OThe S series stereomicroscopes and acces-
sories are operated, maintained and
repaired by authorized and trained person-
nel only.
OAll operators have read, understood and
observe this User Manual, and particularly
the safety instructions.

Leica S Series User Manual 6
Safety Instructions (continued)
Repairs, service work
ORefer to "Safety Concept" booklet
OOnly original Leica Microsystems spare
parts may be used.
OBefore opening the instruments, switch off
the power and unplug the power cable.
Touching the live circuit can cause injury.
Transport
OUse the original packaging for shipping or
transporting the individual modules of the
Leica S stereomicroscopes and the acces-
sory components.
OIn order to prevent damage from vibra-
tions, all moving parts that (according to
the user manual) can be assembled and
disassembled by the customer should be
disassembled and packed separately.
Integration in third-party products
ORefer to "Safety Concept" booklet
Disposal
ORefer to "Safety Concept" booklet
Legal regulations
ORefer to "Safety Concept" booklet
EC Declaration of Conformity
ORefer to "Safety Concept" booklet

Leica S Series User Manual 7
Safety Instructions (continued)
Health risks
•Workplaces with stereomicroscopes facilitate and improve the viewing task,
but they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
OOptimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
OThorough training of the personnel, giving
consideration to ergonomic and organizati-
onal aspects.
OThe ergonomic optical design and const-
ruction of the Leica S stereomicroscopes
are intended to reduce the exertion of the
user to a minimum.
•Direct contact with eyepieces is a potential transmission method for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.

Leica S Series User Manual 8
General Instructions 2
Important Safety Notes 3
Symbols Used 4
Safety Instructions 5
Table of contents 8
Leica S Series
Congratulations! 11
The Modular Design: Everything is Relative 12
What your stereomicroscope has to offer you 13
The model series 14
On We Go 15
Assembly
Installing the basic equipment (overview) 17
Focusing column 18
Sub-base for transmitted light and cold light source 19
Optics carrier and additional objective 20
Available graticules 21
Inserting graticules 22
Eyepieces 23
Leica LED illuminators 24
Mounting a camera (Leica S6 D and S8 APO) 25
Quick Start Guide
A stereomicroscope of the S series in overview 27
Tips for working ergonomically 28
Using the Eyepieces 29
The correct interpupillary distance 30
Focusing 31
Switching magnification (zooming) 32
Limiting zoom range 33
Regulating the resistance of the focusing drive 35
Changing the position of the optics carrier 36
Diopter settings and parfocality: 1 adjustable & 1 fixed eyepiece 37
Dioptric correction with two adjustable eyepieces 40
Photography & Video
Photography & Video 44
Photography with the Leica S6 D and S8 APO 45
Dimensional drawings in mm
Leica S6 E (S4 E / S6 T) with incident light and transmitted light
illumination 47
Leica S6 48
Leica S6 D with incident light and transmitted light illumination 49
Leica S8 APO with incident light and transmitted light illumination 50
Table of Contents

Leica S Series User Manual 9
Specifications
Overview of technical features 52
Specifications 53
Appendix
Calculating the total magnification/field of view diameter 55
Troubleshooting 56
Care, Maintenance, Contact Persons 57

Leica S Series User Manual 10
Leica S series

Leica S Series User Manual 11
Congratulations!
Congratulations on obtaining your new Leica StereoZoom® line stereomi-
croscope (S series). We are convinced it will exceed your expectations, as
this instrument embodies all the qualities you associate with the name
Leica Microsystems: excellent objectives, high-quality engineering, and
reliability. Furthermore, the modular design ensures that the Leica stereo-
microscope adapts perfectly to your needs – no matter which accessories
you require for your tasks.
Thanks to the parfocal system with simultaneously large working distan-
ces and object fields, you can always view your microscopic specimens
accurately – from the complete image to the finest detail.
Though the reliability and robustness of Leica stereomicroscopes is world-
renowned, like any high-tech product, the Leica S series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work! after all, you are now equip-
ped with the best tool!

Leica S Series User Manual 12
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu-
tation when it comes to devising customer-
specific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. He or she has a solution
for every application.
The Modular Design: Everything is possible
The Leica S series provides a high degree of
flexibility in choosing equipment, thanks
primarily to the modular configuration and
the compatibility that Leica has painstakingly
maintained for decades. The optics carriers,
eyepieces, stands and more can be combined in
any way you choose, allowing you to create the
stereomicroscope that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ signi-
ficantly. Whichever configuration you choose,
you will quickly feel right at home with your
new stereomicroscope.

Leica S Series User Manual 13
What your stereomicroscope has to offer you
The optical system of the Leica StereoZoom®
line consists of two beam paths converging at
12°. The objective pairs of each optical path are
positioned close together, so the stereomicro-
scopes can be of very "slender" design, espe-
cially towards the base of the instrument. The
advantage: The advantages of this design are
that it has a small space requirement for use on
bonders and in machine applications, unobst-
ructed access to specimens, plenty of space for
tools and a completely clear view of the object
field.
The Greenough system enables cost-effective
correction of aberrations such as chromasia,
image field curvature, and distortion with mini-
mal effort. In the new Leica StereoZoom® line,
the optimum corrected center of the objec-
tive is used for the image. This provides supe-
rior optical performance with large, level and
undistorted fields of view and chromatically
optimized, high-contrast images.
ESD protection
The Leica S4 E, S6 E, S6, S6 D and S8 APO,
including its cold light source and stand, is
made from highly conductive material with
surface resistivity of 2-10 11 Ohm/square, with a
discharge time of <2 seconds, 1000 V to 100 V.
The Leica S6 T terminator version for highly
complicated working ranges and the T incident
light stand consist of a dissipative material with
a surface resistivity of 102-106Ohm/square and
a discharge time of <0.1 seconds from 1000 V
to zero.
Photography
The StereoZoom® models Leica S6 D and S8
APO are equipped with an integrated video/
phototube, which allows the simple, fast moun-
ting of digital cameras
Apochromatic correction
The Leica S8 APO is a completely apochromati-
cally corrected Greenough system. The modern
optical technology of the StereoZoom® Leica
S8 APO corrects chromatic aberration perfectly,
removes bothersome color seams and displays
pin-sharp images of even the finest enlarged
detail. Contrast, brilliance, image sharpness,
resolution, color fidelity and image precision
are unsurpassed. The benefit of apochromatic
correction is best seen in specimens that have a
fine, low-contrast structure such as large animal
cells, cilia plants or metallic microelectronic
structures.
The technical features of the individual models
are found on page 52.

Leica S Series User Manual 14
The model series
Leica S4 E Leica S6 E Leica S6
Leica S6 T Leica S6 D Leica S8 APO

Leica S Series User Manual 15
On We Go
If your new Leica stereomicroscope has already
been assembled and commissioned by your
Leica consultant, click here to skip through the
installation instructions and go directly to the
Quick Start Guide on Page 26.
If, on the other hand, you are assembling the
stereomicroscope yourself, continue with
the "Assembly" chapter, which begins on
Page 16.

Leica S Series User Manual 16
Assembly

Leica S Series User Manual 17
Installing the basic equipment (overview)
1. Sub-base for transmitted light with glass stage plate
2. Incident light base with stage plate
3. Focusing column with microscope carrier
4. Additional objective, optional
5. StereoZoom® optics carrier
6. Eyepieces, fixed and/or adjustable
6
5
4
3
2
1

Leica S Series User Manual 18
•Never unscrew the 3 screws on the right side of the focusing column.
Focusing column
Focusing column on the incident light base
1. Remove the stage plate.
2. Route the 3 hollow screws from below
through the base plate and screw these
securely into the focusing column.
3. Insert the stage plate back into place.

Leica S Series User Manual 19
1. Remove the glass stage plate.
2. Pull the locking mechanism forward.
3. Set the incident-light stand to the sub-base
for transmitted light and engage it with the
connection screw.
4. Press the locking mechanism backward.
The incident light base and the sub-base
for transmitted light are now connected.
5. Insert the glass stage plate.
6. Insert the universal light guide in the
opening at the rear.
Additional information can be found in the User Manual for cold-light source
Leica KL300 LED.
Sub-base for transmitted light and cold light source

Leica S Series User Manual 20
Optics carrier
1. Insert the optics carrier carefully in the
microscope carrier and fasten it in the desi-
red position with the clamping screw.
Additional objective (optional)
1. Screw the desired objective counterclock-
wise into the optics carrier.
Protective objective glass (optional)
1. Screw the objective protective glass directly
onto the StereoZoom® or on the additional
objective.
Optics carrier and additional objective