Nikon ECLIPSE Ti2-U User manual

M702 E21.2.NF.8 (1/2)
*M702EN08*
Inverted Research Microscope
Instruction Manual

Introduction
i
Introduction
Thank you for purchasing a Nikon product.
This instruction manual is intended for users of the Nikon Inverted Research Microscope ECLIPSE Ti2-U.
To ensure correct usage, read this manual carefully before operating this product.
• No part of this manual may be reproduced or transmitted in any form without prior written permission
from Nikon.
• The contents of this manual are subject to change without notice.
• The equipment described in this manual might differ from the actual product in its appearance.
• Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies
might remain. If you notice any points that are unclear or incorrect, please contact your local Nikon
representative.
• Some of the equipment described in this manual may not be included in the set you have purchased.
• If you intend to use any other equipment with this product, read the manual for that equipment too.
• If the equipment is used in a manner not specified by the manufacturer, the protection provided by the
equipment might be impaired.
• Products and company names mentioned in this manual are trademarks or registered trademarks of
their respective holders.

Contents
ii
Contents
Introduction......................................................................................................................................................i
Safety Precautions .......................................................................................................................................vii
Meaning of Symbols Used on the Product................................................................................... vii
WARNING and CAUTION Symbols Used in This Manual.......................................................... viii
WARNING .............................................................................................................................. ix
CAUTION ............................................................................................................................. xiv
Handling of This Product...........................................................................................................................xvii
Chapter 1 Names of Parts and Their Functions .........................................................................................1
1.1 System Configuration and Names of Components........................................................................2
1.2 Nomenclature and Functions .........................................................................................................4
1.2.1 Dia-illumination Section ....................................................................................................4
1.2.2 Condenser Unit and Polarizer...........................................................................................5
1.2.3 Tube and Eyepiece ...........................................................................................................6
1.2.4 Tube Base Unit .................................................................................................................6
1.2.5 Stage.................................................................................................................................7
1.2.6 Nosepiece .........................................................................................................................8
1.2.7 Objectives .........................................................................................................................8
1.2.8 FL Turret and Analyzer......................................................................................................9
1.2.9 Epi-fluorescence Attachments ........................................................................................10
1.3 Operation Sections of the Microscope Main Body .......................................................................12
1.3.1 Front Operation Panel ....................................................................................................12
1.3.2 Right and Left Operation Panels.....................................................................................12
1.3.3 Power Switch ..................................................................................................................13
1.3.4 AC Adapter and Power Cord ..........................................................................................13
Chapter 2 Microscopy .................................................................................................................................15
2.1 Diascopic Bright-field (BF) Microscopy ........................................................................................16
2.2 Diascopic Phase Contrast (Ph) Microscopy.................................................................................18
2.3 Diascopic DIC / IMSI Microscopy.................................................................................................20
2.4 Episcopic Fluorescence (Epi-FL) Microscopy..............................................................................22
2.5 Diascopic Dark-field (DF) Microscopy..........................................................................................24
2.6 NAMC Microscopy .......................................................................................................................26
Chapter 3 Usage of Components ...............................................................................................................29
3.1 Power-related Operation ..............................................................................................................30
3.2 Optical Parts in the Microscope Main Body .................................................................................31
3.2.1 Switching the Output Port ...............................................................................................31
3.2.2 Switching the Magnification of the Device with the Intermediate Magnification Dial......32
3.3 Using the Dia-illumination Section ...............................................................................................33
3.3.1 Opening/Closing the Dia-Illumination Shutter.................................................................33
3.3.2 Using Diascopic Illumination...........................................................................................33
3.3.3 Adjusting the Dia Field Diaphragm .................................................................................34
3.3.4 Using a Filter for Dia-illumination....................................................................................35
3.3.5 Using the Dia-illumination Pillar ......................................................................................36

Contents
iii
3.3.6 Operating the Contrast Shield (Optional) .......................................................................37
3.3.7 Using the D-LH/LC Precentered Lamphouse .................................................................38
3.4 Using the Condenser Section ......................................................................................................41
3.4.1 Selecting a Suitable Condenser .....................................................................................41
3.4.2 Elevating the Condenser ................................................................................................42
3.4.3 Adjusting the Position of the Condenser (Centering) .....................................................42
3.4.4 Using an Oil Immersion Condenser Lens.......................................................................43
3.4.5 Adjusting the Diascopic Aperture Diaphragm .................................................................44
3.4.6 Switching the Condenser Module ...................................................................................45
3.4.7 Adjusting the Ph Module Position ...................................................................................47
3.4.8 Adjusting the NAMC Module...........................................................................................48
3.4.9 Using the ELWD-S Condenser .......................................................................................50
3.5 Using the Polarizer and Analyzer .................................................................................................51
3.5.1 Using the Polarizer..........................................................................................................51
3.5.2 Using the Analyzer..........................................................................................................53
3.6 Using the Tube and the Objective................................................................................................54
3.6.1 Adjusting the Angle of the Binocular Part (ER tube).......................................................54
3.6.2 Adjusting the Diopter of the Eyepiece.............................................................................55
3.6.3 Adjusting Interpupillary Distance ....................................................................................56
3.6.4 Operating the Centering Telescope ................................................................................56
3.7 Using the Tube Base Unit ............................................................................................................57
3.7.1 Switching the Optical Path of the Tube Base Unit (Tube Base Unit with Port)...............57
3.8 Using the Stage............................................................................................................................58
3.8.1 Setting a Sample on the Manual Stage ..........................................................................58
3.8.2 Setting a Sample on the Plain Stage/Gliding Stage .......................................................59
3.8.3 Moving a Sample on the Stage.......................................................................................60
3.9 Using the Nosepiece ....................................................................................................................61
3.9.1 Turning the Nosepiece to Switch the Objective ..............................................................61
3.9.2 Placing the DIC Prism into the Optical Path ...................................................................61
3.10 Using the Objective ......................................................................................................................62
3.10.1 Adjusting the Objective with the Correction Collar .........................................................62
3.10.2 Using Oil Immersion Objectives......................................................................................64
3.10.3 Using a Water Immersion Objective ...............................................................................65
3.10.4 Using an Objective with a Diaphragm.............................................................................66
3.11 Using the Focusing Device ..........................................................................................................67
3.11.1 Focusing on the Sample .................................................................................................67
3.11.2 Using the Coarse-Focus Knob Torque Adjustment Ring ................................................68
3.11.3 Using the Objective Refocusing Lever ...........................................................................69
3.12 Using the FL turret........................................................................................................................70
3.12.1 Switching the Filter Cube................................................................................................70
3.12.2 Using the Shutter of the FL Turret ..................................................................................70
3.12.3 Selecting Filter Cubes.....................................................................................................71
3.13 Using the Epi-fluorescence Attachment .......................................................................................72
3.13.1 Adjusting the Aperture Diaphragm of the Epi-fluorescence Attachment.........................72
3.13.2 Using the Filter Slider......................................................................................................73
3.13.3 Using the Field Diaphragm Slider ...................................................................................74
3.13.4 Switching the Optical Path of the Main Branch ..............................................................76
3.14 Using a Back Port Unit (Optional) ................................................................................................77

Contents
iv
3.15 Using a Centering C-mount Adapter ............................................................................................78
Chapter 4 Microscopy Techniques ............................................................................................................79
4.1 Details of Diascopic Bright-field (BF) Microscopy ........................................................................80
4.1.1 Principles of BF Microscopy ...........................................................................................80
4.1.2 Optical Elements Required for BF Microscopy...............................................................81
4.1.3 Combination of Optical Elements ...................................................................................82
4.2 Details of Phase Contrast (Ph) Microscopy .................................................................................83
4.2.1 Principles of Ph Microscopy............................................................................................83
4.2.2 Optical Elements Required for Ph Microscopy ...............................................................84
4.2.3 Combination of Optical Elements ...................................................................................86
4.3 Details of Diascopic DIC and IMSI Microscopies.........................................................................87
4.3.1 Principles of DIC Microscopy..........................................................................................87
4.3.2 Optical Elements Required for DIC Microscopy .............................................................88
4.3.3 Combination of Optical Elements ...................................................................................90
4.4 Details of Episcopic Fluorescence (Epi-FL) Microscopy..............................................................94
4.4.1 Principles of Epi-FL Microscopy......................................................................................94
4.4.2 Optical Elements Required for Epi-FL Microscopy .........................................................95
4.4.3 Combination of Optical Elements ...................................................................................96
4.5 Details of Diascopic Dark-field (DF) Microscopy..........................................................................97
4.5.1 Principles of DF Microscopy ...........................................................................................97
4.5.2 Optical Elements Required for DF Microscopy...............................................................97
4.5.3 Combination of the Optical Elements Required for DF Microscopy ...............................99
4.6 Details of NAMC Microscopy .....................................................................................................100
4.6.1 Principles of NAMC Microscopy ...................................................................................100
4.6.2 Optical Elements Required for NAMC Microscopy.......................................................101
4.6.3 Combination of Optical Elements .................................................................................102
Chapter 5 Troubleshooting .......................................................................................................................103
5.1 Troubleshooting for Microscopy Techniques..............................................................................104
5.1.1 Common Microscopy Troubles .....................................................................................104
5.1.2 Troubles in BF Microscopy ...........................................................................................107
5.1.3 Troubles in Ph Microscopy............................................................................................109
5.1.4 Troubles in DIC and IMSI Microscopies .......................................................................110
5.1.5 Troubles in Epi-FL Microscopy ..................................................................................... 111
5.1.6 Troubles in DF Microscopy ........................................................................................... 111
5.1.7 Troubles in NAMC Microscopy .....................................................................................112
5.2 Troubleshooting for the Units .....................................................................................................113
5.2.1 Microscope Main Body .................................................................................................113
5.2.2 Problems with the Dia-illumination Section ..................................................................113
5.2.3 Pillar for Dia Illumination and Condenser .....................................................................114
5.2.4 Stage.............................................................................................................................114
5.2.5 Nosepiece and Focusing Device ..................................................................................114
5.2.6 Epi-fluorescence Attachment ........................................................................................114
Chapter 6 Maintenance and Storage of the Device ................................................................................115
6.1 Cleaning the Device ...................................................................................................................116
6.1.1 Cleaning the Lenses .....................................................................................................117
6.1.2 Cleaning Parts other than Lenses ................................................................................117

Contents
v
6.1.3 Cleaning Off Immersion Oil...........................................................................................117
6.1.4 Decontaminating the Product .......................................................................................117
6.2 Storing the Product ....................................................................................................................118
6.3 Regular Inspection (Charged) ....................................................................................................118
Chapter 7 Assembly of the Devices.........................................................................................................119
7.1 Ti2-U System Configuration .......................................................................................................121
7.2 List of Components ....................................................................................................................123
7.3 Installing the Microscope Main Body (Base)..............................................................................127
7.3.1 Removal and Installation ..............................................................................................127
7.4 Mounting the Tube Base Unit.....................................................................................................128
7.5 FL Turret (Required for Epi-FL Microscopy)...............................................................................129
7.5.1 Attaching an FL Turret ..................................................................................................129
7.5.2 Mounting a Filter Cube .................................................................................................130
7.5.3 Replacing an Excitation Filter, a Barrier Filter, and a Dichroic Mirror ...........................131
7.5.4 Attaching Optical Elements to the C-FL-HQ Filter Cube ..............................................135
7.6 Attaching a Nosepiece ...............................................................................................................137
7.7 Mounting a Stage .......................................................................................................................138
7.7.1 Mounting the Stage.......................................................................................................138
7.7.2 Attaching a Stage Handle (Manual Stage Only)...........................................................139
7.7.3 Removing a Fixing Tool of the Stage ............................................................................141
7.7.4 Restriction on Stage Strokes (Manual Stage Only) ......................................................142
7.8 Mounting a Diascopic Illumination Pillar and a Lamphouse ......................................................143
7.8.1 Mounting an Illumination Pillar......................................................................................143
7.8.2 Attaching the Condenser Holder...................................................................................144
7.8.3 Attaching a Fixed Filter for Dia-illumination ..................................................................145
7.8.4 Attaching a Filter Slider for Dia Illumination (Optional).................................................146
7.8.5 Attaching a Motorized Dia-Illumination Shutter (Optional)............................................147
7.8.6 Mounting a Lamphouse for Dia-illumination .................................................................148
7.8.7 Replacing a Dia-Illumination Lamp (Precentered Lamphouse only) ............................149
7.9 Attaching a Contrast Shield (Optional).......................................................................................150
7.10 Attaching an Analyzer and a Polarizer (Required for DIC Microscopy) .....................................151
7.10.1 Attaching an Analyzer ...................................................................................................151
7.10.2 Attaching a Polarizer.....................................................................................................152
7.11 Attaching a Condenser...............................................................................................................153
7.11.1 Preparation for Attaching a Condenser.........................................................................153
7.11.2 Attaching a Condenser Turret (System Condenser).....................................................154
7.11.3 Attaching an ELWD-S Condenser ................................................................................157
7.11.4 Attaching a DF Condenser ...........................................................................................157
7.11.5 Attaching a High NA Condenser ...................................................................................158
7.12 Attaching the Tube and Eyepieces ............................................................................................160
7.13 Attaching an Epi-fluorescence Attachment ................................................................................161
7.13.1 Mounting an EPI-FL Module (TI2-LA-FL-2) ..................................................................161
7.13.2 Attaching a Field Diaphragm Slider ..............................................................................165
7.14 Attaching a Camera Device .......................................................................................................166
7.14.1 Attaching a Camera Device to the Side Port (Microscope Main Body)........................166
7.14.2 Attaching a Camera Device to the Side Port on the Tube Base Unit ...........................168
7.14.3 Attaching the Back Port Unit (Optional)........................................................................169
7.15 Connection of Each Cable .........................................................................................................170

Contents
vi
7.15.1 Position of Each Connector ..........................................................................................170
7.15.2 Clamping Cables...........................................................................................................171
7.16 Attaching Objectives ..................................................................................................................172
7.16.1 Attaching CFI60 Objectives ..........................................................................................172
7.16.2 Parfocal Adjustment of Objectives without Correction Collar .......................................173
7.17 Attaching a Specimen Holder.....................................................................................................175
7.17.1 Attaching a Specimen Holder for the Manual Stage.....................................................175
7.17.2 Attaching a Stage Ring .................................................................................................175
7.18 Moving the Microscope ..............................................................................................................176
Chapter 8 Specifications...........................................................................................................................177
8.1 Principles of Operation...............................................................................................................178
8.2 Performance Properties .............................................................................................................179
8.3 Physical Properties ....................................................................................................................183

Safety Precautions
vii
Safety Precautions
Although this product is designed and manufactured to be completely safe during use, incorrect usage or
failure to follow the safety instructions provided may cause personal injury or property damage. To ensure
correct usage, carefully read this manual and the instruction manuals of products used together before using
the product. Be sure to read and observe the safety precautions described at the beginning of each manual.
Do not discard this manual and keep it handy for easy reference.
Meaning of Symbols Used on the Product
The symbol appearing on the product indicates the need for caution at all times during use. Always refer to
the instruction manual and read the relevant instructions before manipulating any part to which the symbol
has been affixed.
Symbol Description
Biohazard
This symbol label is attached on the top or front surface of the stage to remind the user of the
following precautions:
• Spillage of a specimen from a vessel onto the microscope, presents a biohazard risk.
• To avoid biohazard contamination, do not touch the contaminated portion with your bare hands.
• Decontaminate the contaminated portion according to the standard procedure of your laboratory.
Manual stage Plain stage Gliding stage
Caution for heat
This symbol label is attached on the top surface of the optical path cover on the top of the
illumination pillar, on the top surface of the TI2-D-SF filter slider for dia illumination, and on the
back surface of the D-LH/LC precentered lamphouse. When the D-LH/LC is used as the
dia-illumination source, this symbol reminds the user of the following precautions:
• The lamp and lamphouse, the top surface of the optical path cover, and the top surface of the
filter slider for dia-illumination become hot while the lamp is lit or immediately after it goes out.
• To avoid the risk of burns, do not touch the lamp or lamphouse, the top surface of the optical
path cover, or the top surface of the filter slider for dia-illumination while the lamp is lit or
immediately after it goes out.
• Make sure the lamp, the lamphouse, and the air vents of the HG precentered fiber illuminator
have cooled sufficiently before attempting to replace the lamp.
S.
F.
The top surface of the optical path
cover
S.
F.
The top surface of
the TI2-D-SF filter slider for dia
illumination
Rear surface of the D-LH/LC
precentered lamphouse
When a TI2-D-LHLED LED
lamphouse for dia-illumination is used
When the TI2-D-SF filter slider for dia illumination and the D-LH/LC
precentered lamphouse are used
Biohazard symbol
Biohazard
symbol Biohazard
symbol
Hot surface
symbol
Hot surface
symbol
Hot surface
symbol

Safety Precautions
viii
Symbol Description
Caution for heat
This symbol label is attached on the front and top
surfaces of the motorized shutter unit to remind the
user of the following precautions:
• The motorized shutter may become hot if closed
during a period of illumination.
• Burn risk. Do not touch the motorized shutter
during and immediately after a period of
illumination.
Motorized shutter
Warning (photobiological safety)
This symbol label is attached on the side surface of
the pillar for dia-illumination to remind the user of the
following precautions:
• Classification of dia-illumination and fluorescent
illumination according to the photobiological risk
groups
• Do not attempt to view dia-illumination or
fluorescent illumination.
For details, see “10. Photobiological safety” and “11.
Do not attempt to view the illumination section.”
under WARNING.
S.
F.
Pillar for dia-illumination (top side surface)
Caution (filter cube replacement port)
This symbol label is attached near the filter cube
replacement port of the FL turret to remind the user
of the following precautions:
• Be sure to close the cover of the filter cube
replacement port because light including ultraviolet
light might leak from the port.
• For details, see “12. Cautions on replacing the filter
cube” under WARNING.
Close the shutter
before opening the cover
of the filter cube slot.
C
FL turret
WARNING and CAUTION Symbols Used in This Manual
Safety instructions in this manual are marked with the following symbols to highlight their importance. For
your safety, always follow the instructions marked with these symbols.
Symbol Description
WARNING Disregarding instructions marked with this symbol may lead to serious injury or death.
CAUTION Disregarding instructions marked with this symbol may lead to injury or property
damage.
In addition, the following symbols are used in this manual:
Symbol Meaning
This symbol indicates instructions required to avoid malfunction and failure of the
product and damage to the equipment or parts.
This symbol indicates necessary information and tips on the use of this product.
Hot surface
symbol
Hot surface
symbol
Warning
symbol
Caution
symbol
Filter cube
replacement port