Emulate ORB-HM1 User manual
TM
hub module
TM
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Orb™Hub Module
Gas, Energy, Stretch.
Our Orb Hub Module is a central hub that connects to up to four Zoë™ Culture Modules.
It provides a simple solution for installing and operating our system within the lab environment.
The sphere acts as a transformer by harvesting CO2and electricity from standard lab
connections, and outputs the precise balance of air and energy required by our Zoë
Culture Modules for optimum cell culture performance.
The Orb Hub Module also supplies the vacuum pump functionality required for automated
control of the dynamic mechanical forces the Zoë Culture Modules require for Organ-Chips
that require breathing motions or peristalsis — such as the lung or intestine.
Our work is directed towards a clear goal — to democratize Organs-on-Chips technology and
create an easy-to-use research platform that emulates human biology for understanding how
diseases, medicines, chemicals, and foods, aect human health.
This user guide contains important information to safely and eectively operate the Orb-HM1™ instrument.
Please ensure that all users thoroughly read and understand this guide before operation.
This product is for research use only.
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Introduction
System Components
Specifications
General, Functional & Technical
Safety
Information & Precautions
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Introduction
User Guide
Table of Contents
Orb™Hub Module (HM1) / Table of Contents
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Installation
Equipment & Procedure
Use
Interface & Control Panel
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Use
Care
Maintenance & Troubleshooting
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Introduction
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1
This section contains an overview of the Orb™Hub Module
and its functions. The Orb Hub Module provides the power,
gas mixture, and vacuum required for Organ-Chip cell culture.
It can generate the appropriate gas mixture from an external
100% carbon dioxide supply, or from a portable carbon dioxide
canister for increased flexibility. The Orb Hub Module can
support up to four Zoë™Culture Modules simultaneously, which
equates to up to 48 Organ-Chips.
Orb-HM1™
System Components
Orb™Hub Module (HM1) / Introduction
The Orb™ Hub Module provides all the necessary elements to power and
run up to four Zoë™ Culture Modules at once. These elements include
mixed gas, vacuum, and power. The Orb Hub Module takes 100% carbon
dioxide from one of two sources, mixes the CO2with air, and outputs a gas
mixture that contains 5% CO2. The CO2sources can be a direct line from an
external 100% CO2source, such as one that provides CO2to incubators, or a
portable CO2canister. The CO2canister is installed directly onto the Orb Hub
Module in the center of the Control Panel. The Orb Hub Module generates
the vacuum required to apply Organ-Chip stretching, and contains four
individual power ports.
Important note: this user guide covers the basic operation of the Orb
Hub Module. For specific protocols on using the Organ-Chips and Pod™
assemblies to culture cells, please refer to the appropriate Organ-Chip
documentation.
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Orb™Hub Module
(Front)
Control Panel
(Rear)
Provides the gas mixture, vacuum,
and power to run up to four Zoë™
Culture Modules.
All ports are located on the rear
Control Panel. The Orb Hub Module
can utilize an external CO2source or
a portable CO2canister.
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Orb-HM1™
Components
Orb™Hub Module (HM1) / Introduction
1. Orb™ Hub Module
2. Main Status Indicator
3. Drip Tray
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Orb-HM1™
Control Panel
1. Power Ports
2. CO2Canister Connection
3. Alarm Silence Button
4. Mixed CO2Out
5. Vacuum Out
6. 100% CO2In
7. Power Input & On / O Switch
GAS OUT
VAC OUT
GAS IN
POWER
3 & 4
POWER
1 & 2
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Specifications
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This section includes specific technical information and
operation requirements, such as system dimensions,
temperature, and gas. Manufacturer and contact information
is included here as well.
Orb-HM1™
Specifications
Orb™Hub Module (HM1) / Specifications
Manufacturer Emulate, Inc., 27 Drydock Avenue, Boston, MA 02210
Assistance For technical assistance, contact Emulate, Inc., support@emulatebio.com
Use The Orb™Hub Module is for use with the Zoë-CM1™. The Orb Hub Module is for research use only.
13.0 in13.0 in
11.75 in
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General Model: HM1
Weight: 7.3 kg (16.0 lbs)
Power Consumption: 105W
Operating Electrical Power: 100–240VAC, 50–60Hz
Requirements Gas Input Pressure: 68.9–137.9 kPa from fixed source / 20,684.3 kPa from 68-gram gas canister
(10–20 psig from fixed source / 3,000 psig from 68-gram gas canister)
Gas Input Composition: 100% CO2
Environmental Operating Temperature: 10–35ºC (50-95ºF)
Relative Humidity: 30–80% RH
Max Altitude: 2,000 meters (6,562 feet)
Storage Temperature: -5–60˚C
Storage Humidity: 30–90% RH, non-condensing
IP Rating: IP12B
Technical Gas Output Composition: 5% CO2, balance air
Gas Output Pressure: 275.8 kPa + / - 34.5 kPa (40 psig + / - 5 psig)
Max Mixed Gas Flowrate: 130 mL / minute
Vacuum Output: -70 kPa (-10.2 psig)
Electrical Output: Four 12VDC ports power up to four Zoë™Culture Modules
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Safety
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This section includes precautions for handling and using the
Orb™Hub Module. The information here describes how users
can minimize any chance of harming themselves or the Orb
Hub Module during use. Additionally, regulatory compliance
information is contained in this section.
Orb™Hub Module (HM1) / Safety
Safety
The Instructions Symbol
The product is marked with this warning symbol where it is necessary for the user to refer to the instructions in the user guide.
Regulatory Compliance and Testing
This product has been tested to the requirements of:
61010-1 (IEC, EN, CSA) Safety Requirements for Electrical Equipment for Laboratory Use
EMC Directive 2014 / 30 / EU, LVD Directive 2014 / 35 / EU
EN 61326-1 Electro-Magnetic Compliance (EMC)
