entegris Aramus SU-2D Series User manual

ADVANCED MATERIALS HANDLING |MANUAL
Aramus™Standard
Bag Assemblies
User manual

1
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
TABLE OF CONTENTS
—
Introduction ................................................................... 2
Safety ............................................................................... 2
Aramus Standard Bag Subassemblies ........................ 3
Bag Description .............................................................. 3
Removing the Aramus Subassembly
from its Packaging ......................................................... 3
Connecting Bags ........................................................... 3
Filling and Freezing Bags ............................................. 3
Storage, Handling, and Shipping ............................... 3
Aramus Standard Bag Assemblies .............................. 3
Removing the Full Aramus Assembly
from its Packaging ......................................................... 4
Hanging the Full Aramus Assembly .......................... 4
Connecting Bags ........................................................... 4
Filling and Freezing Bags ............................................. 4
Storage, Handling, and Shipping ............................... 5
Specifications for Aramus Assembly
Standard Components ................................................. 5
Configurations ................................................................ 6
Ordering Information ................................................... 7
Aramus Standard Bag Assembly
with RoSS®Shell ......................................................... 10
Specifications ................................................................. 11
Shipping ......................................................................... 12
Inspection ...................................................................... 12
Assembly Instructions ................................................ 12
Ordering Information ................................................. 14
Aramus Standard Bag Assembly
with Cassettes .............................................................. 15
Specifications ................................................................ 16
Warnings ........................................................................ 16
Shipping ......................................................................... 17
Inspection ...................................................................... 17
Assembly Instructions ................................................ 17
Ordering Information ................................................. 19
RoSS Shell and Cassette Disclaimer ........................ 19
Limited Warranty ........................................................ 20
For More Information ................................................ 20
Terms and Conditions of Sale .................................. 20

2
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
INTRODUCTION
—
Standard Aramus™single-use 2D bag assemblies are
made of a high-grade, gamma-stable fluoropolymer,
providing higher purity, greater compatibility, and
increased safety for critical process fluids and final
products. With a new single-layer technology that
contains no curing agents, antioxidants, plasticizers,
or adhesives, the number of potential contaminants
is greatly reduced. These assemblies oer the widest
operating temperature range, making them more
durable in frozen applications (to -85°C [-121°F] or
lower) without negatively aecting the film.
Additionally, the fluoropolymer film oers universal
material compatibility, reducing the risk of material
and assembly failure. Aramus single-use assemblies
are manufactured in an ISO®Class 5 cleanroom using
methodologies that go beyond the standard. These
assemblies allow for easier validation and provide
high purity, performance, quality, and safety for
your process.
This user manual describes the instructions for use
for the Aramus bag and assemblies.
SAFETY
—
Before using any Aramus single-use assembly or
subassembly, you should read, understand, and
follow the instructions in this manual. Save these
instructions and make them available to users of
this product.
To prevent injury, follow these safety procedures:
• Do not hang bags on anything that cannot support
the weight; doing so can cause injury and/or
equipment damage from tipping over.
• Do not use the tubing to handle or carry the bags.
• Do not use this product in a manner inconsistent
with its intended use.
• Follow all appropriate chemical safety and disposal
regulations.
• Wear appropriate personal protective equipment
when handling components containing chemicals.
• Over pressurizing this product may cause injury
from flying debris and/or exposure to chemicals.
• DO NOToverfill this product. Overfilling may lead
to a spill and chemical exposure.
• Regularly inspect the product for leaks or other
damage.
• Please note that the user shall be solely responsible
and liable for validation and use of Aramus bags
beyond the range of our specifications for unit
operations, applications, and process parameters.

ARAMUS STANDARD BAG ASSEMBLIES
3User Manual | Rev. B 03/20 | Entegris, Inc.
ARAMUS SUBASSEMBLIES
—
If you ordered the full assembly, skip to Aramus
Standard Bag Assemblies on page 4.
Entegris oers the Aramus bag assembly in a sub-
assembly configuration; meaning without tubing,
connectors, or sterilization.
BAG DESCRIPTION
Aramus subassemblies are oered in 2D configura-
tions in various sizes with a 2- or 3-port boat fitment.
See Figure 1 for Aramus subassembly bag configu-
ration. See Ordering Information on page 9 for the
standard part numbers available; customization is
available upon request.
REMOVING THE ARAMUS SUBASSEMBLY FROM
ITS PACKAGING
CAUTION: DO NOT use anything sharp to remove
the Aramus subassembly from its packaging.
1. The Aramus subassemblies will arrive in a corru-
gated box. DO NOT use anything sharp to open
the box. Remove packing tape with caution to
avoid damaging the contents.
2. There are two sealed bags protecting the Aramus
subassembly. To open the outer bag, grip the side
of the protective packaging bag and use the “easy
tear" notches (see Figure 2) to pull in opposite direc-
tions, allowing the top section and main body of
the packaging to separate and subsequently open
the bag. Repeat this procedure to open the inner
protective package.
CONNECTING BAGS
Attach appropriately sized tubing to the hose barb
connections found on the boat fitment. The tubing
can then be combined with your desired connecting
piece or method.
FILLING AND FREEZING BAGS
The specified Aramus bag volume is the actual fill
volume the bag is capable of holding. The bags
are rated to 110% of the nominal volume to avoid
overfilling. For best freezing, it is recommended
that the Aramus bag be laid flat or in a horizontal
position and should be filled to no more than the
maximum rated volume.
STORAGE, HANDLING, AND SHIPPING
The Aramus subassembly has a recommended stor-
age temperature of 18° to 30°C (64° to 86°F), but due
to its material of construction, has a performance
temperature rating of -85° to 40°C (-121° to 104°F).
Handle with care to avoid dropping and when hang-
ing the bag vertically, ensure that it is hung securely.
To reduce the risk of damage, filled bags should be
stored, handled, and shipped in a secondary protec-
tive container. DO NOT use bubble wrap around filled
and frozen bags for shipping.
3-port Subassembly
2-port Subassembly
Figure 1. Aramus subassembly bag configurations
Figure 2. Easy tear notches
Easy tear
notches

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ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
ARAMUS STANDARD BAG ASSEMBLIES
—
Entegris oers the Aramus bag in a full assembly with
your desired configuration of tubing and connectors,
delivered sterilized with a Sterility Assurance Level
(SAL) of 10-6.
REMOVING THE FULL ARAMUS ASSEMBLY FROM
ITS PACKAGING
CAUTION: DO NOT use anything sharp to remove
the Aramus assembly from its packaging.
1. The Aramus assembly will arrive in a corrugated
box. DO NOTuse anything sharp to open the
box. Remove packing tape with caution to avoid
damaging the contents.
2. There are two sealed bags protecting the Aramus
assembly. To open the outer bag, grip the side of
the protective packaging bag and use the “easy
tear" notches (see Figure 3) to pull in opposite
directions, allowing the top section and main
body of the packaging to separate and subse-
quently open the bag. Repeat this procedure
to open the inner protective package.
HANGING THE FULL ARAMUS ASSEMBLY
The Aramus assembly has a convenient and
durable hanging slot, as shown in Figure 4.
CONNECTING BAGS
The standard mating hardware oered are Luer
connections and MPC connections. Please follow
the manufacturer’s guidelines when connecting to
these components. The pinch clamp must be in the
released position to ensure flow within the bag.
FILLING AND FREEZING BAGS
The specified Aramus bag volume is the actual
fill volume the bag is capable of holding. The bags
are rated to 110% of the nominal volume to avoid
overfilling.
For best freezing, it is recommended that the Aramus
bag be laid flat or in a horizontal position and should
be filled to no more than the maximum rated volume.
Easy tear
notches
Figure 3. Easy tear notches
Figure 4. Convenient hanging slot

ARAMUS STANDARD BAG ASSEMBLIES
5User Manual | Rev. B 03/20 | Entegris, Inc.
STORAGE, HANDLING, AND SHIPPING
The components on the full Aramus assembly are
commonly used in the market and have various
temperature claims. Entegris has tested the Aramus
bag assemblies while using these components.
The Aramus assembly has a recommended storage
temperature of 18° to 30°C (64° to 86°F), but due
to its material of construction, has a performance
temperature rating of -85° to 40°C (-121° to 104°F).
Handle with care to avoid dropping and when hang-
ing the bag vertically, ensure that it is hung securely.
To reduce the risk of damage, filled bags should be
stored, handled, and shipped in a secondary protec-
tive container. DO NOT use bubble wrap around filled
and frozen bags for shipping.
SPECIFICATIONS FOR ARAMUS ASSEMBLY
STANDARD COMPONENTS
Specifications for the components found on
full Aramus assemblies can be located on the
supplier websites:
Component supplier Website
Colder Product Company
MPC connections
https://www.cpcworldwide.com/
Saint-Gobain
C-Flex®tubing, platinum-cured silicone
tubing, BarbLock®, and pinch clamps
https://www.saint-gobain-northamerica.com/business/markets/life-sciences
Oetiker
Stepless®clamps
http://www.oetiker.com/
Nordson Medical
Luer connections
http://www.nordsonmedical.com/
AdvantaPure
AdvantaFlex®and AdvantaSil®tubing
http://www.advantapure.com/

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ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
CONFIGURATIONS
Full Aramus bag assemblies are oered in 2D con-
figurations in various sizes with a 2- or 3-port boat
fitment and the selected configuration of tubing and
ITEM DESCRIPTION
1Aramus fluoropolymer film with slot for hanging
2Fluoropolymer "boat" fitment
with 2 hose barb ports
3Tubing connection (BarbLock clamp, Oetiker
Stepless clamp, or zip ties*)
4Tubing ⁄" ID × ⁄" OD
(AdvantaFlex thermoplastic elastomer [TPE],
AdvantaSil silicone, Tygon®silicone, or
C-Flex 374 elastomer)
5Pinch clamps
6Tubing fittings ⁄" ID
(Luer male/female with plugs)
connectors. See Ordering Information on page 7 for
the standard part numbers available; customization
is available upon request.
ITEM DESCRIPTION
1Aramus fluoropolymer film with slot for hanging
2Fluoropolymer "boat" fitment
with 3 hose barb ports
3Tubing connection (BarbLock clamp, Oetiker
Stepless clamp, or zip ties*)
4Tubing for 500 mL, 1 L, and 2 L is ⁄" ID × ⁄" OD
Tubing for 5 L, 10 L, 20 L, and 50 L* is ⁄" ID × ⁄"
OD (AdvantaFlex TPE, AdvantaSil silicone, Tygon
silicone, or C-Flex 374 elastomer)
5Tubing for 500 mL, 1 L, and 2 L is ⁄" ID × ⁄" OD
Tubing for 5 L, 10 L, 20 L, and 50 L* is ⁄" ID × ⁄"
OD (AdvantaFlex TPE, AdvantaSil silicone,
Tygon silicone, or C-Flex 374 elastomer)
6Pinch clamps
7Tubing fittings (CPC MPC male/female
with plugs)
8Tubing fittings (Luer male/female with plugs)
9Rod (5 L, 10 L, 20 L, and 50 L*)
Full Aramus Assembly
Configuration Options
2-port: 20 mL, 50 mL, 100 mL, 250 mL
1
2
5
3
6
4
1
2
3
6
4
3
7
8
5
9
Full Aramus Assembly
Configuration Options
3-port: 500 mL, 1 L
Full Aramus Assembly
Configuration Options
3-port: 2 L (no rod),
5 L, 10 L, 20 L, 50 L* (with rod)
5
4
Note: See Ordering Information for the standard part numbers available; customization available upon request.
*Not a standard product, but is available upon request.

ARAMUS STANDARD BAG ASSEMBLIES
7User Manual | Rev. B 03/20 | Entegris, Inc.
ORDERING INFORMATION
Aramus Standard Bag Assemblies (2-port): part number
SU-2D- -
Notes: Assemblies sold sterilized. Assembly temperature
performance is component-dependent. Assemblies are
individually double-bagged.
*Not a standard product, but is available upon request.
Fasteners
O = Oetiker SS
ear clamps
B = BarbLock
clamps
Z = Zip ties*
Product family
SU= Single-use
Product type
2D= 2D bag
Volume
0.020 = 20 mL
0.050 = 50 mL
0.100 = 100 mL
0.250 = 250 mL
Tube material
F = AdvantaFlex thermoplastic elastomer
P = AdvantaSil silicone
S = Tygon silicone
T = C-Flex 374 elastomer
Tube length
0 = 6"
1 = 12"
2 = 18"
3 = 24"
Main connectors
L11 = Luer–1: female 1: female
L12 = Luer–1: female 2: male
L22 = Luer–2: male 2: male

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ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
ORDERING INFORMATION (CONTINUED)
Aramus Standard Bag Assemblies (3-port): part number
SU-2D- -
B
A
B
A
Product family
SU = Single-use
Product type
2D= 2D bag
Volume
00.5 = 500 mL
0001 = 1 L
0002 = 2 L
0005 = 5 L
0010 = 10 L
0020 = 20 L
0050 = 50 L*
Tube length
0 = 6"
1 = 12"
2 = 18"
3 = 24"
Main connectors
CFM = MPC – A: female B: male
CFF = MPC – A: female B: female
CMF = MPC – A: male B: female
CMM = MPC – A: male B: male
Note: See drawing below for A and B indicators
for part numbers
Sample connectors
1 = Luer female
2 = Luer male
Notes: Assemblies sold sterilized. Assembly temperature
performance is component-dependent. Assemblies are
individually double-bagged.
*Not a standard product, but is available upon request.
Fasteners
O = Oetiker SS
ear clamps
B = BarbLock
clamps
Z = Zip ties*
Tube material
F = AdvantaFlex thermoplastic elastomer
P = AdvantaSil silicone
S = Tygon silicone
T = C-Flex 374 elastomer

ORDERING INFORMATION (CONTINUED)
Aramus Subassemblies (2-port): part number
SU-2D- -U
Product family
SU= Single-use
Product type
2D= 2D bag
Volume
0.020= 20 mL
0.050= 50 mL
0.100 = 100 mL
0.250 = 250 mL
Note: Subassemblies sold unsterilized.
Aramus Subassemblies (3-port): part number
SU-2D- -U
Product family
SU= Single-use
Product type
2D= 2D bag
Volume
00.5 = 500 mL
0001 = 1 L
0002= 2 L
0005= 5 L
0010 = 10 L
0020= 20 L
0050= 50 L*
Note: Subassemblies sold unsterilized.
*Not a standard product, but is available upon request.
ARAMUS SINGLE-USE 2D BAG ASSEMBLIES
9User Manual | Rev. B 03/20 | Entegris, Inc.

10
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
ARAMUS STANDARD BAG ASSEMBLY WITH ROSS®SHELL
—
Aramus single-use bag assembly, combined with the
RoSS (Robust Storage and Shipping) shell, provide
greater protection during handling, more consistent
freezing/thawing, and reduced storage density. The
following instructions explain how to most eectively
use the two together for filling, freezing, handling,
and thawing applications.
Please use the correct Aramus bag size and RoSS shell
combination. If filling to maximum volume, remove
excess air from bag to prevent the bag from over
expanding within the RoSS shell. Fill the bags to
greater than 30% of their maximum fill volume stated
in the specification table, page 11. For example, 30%
of a 10 L bag's maximum fill volume is 2.3 L (7.6 x 0.3).
To prevent adverse freezing conditions, do NOT fill
bags above their maximum fill volume, or below 30%
of this volume. This information is for guidance purposes
only. Optimal fill volumes and ranges should be
determined by the customer for each individual
product, freezing method, freezer type, and profile.
CAUTION: Failure to confirm fit for use under
the customer’s requirements may result in
product failure.
NOTE: Additional accessories such as foam inserts may
be necessary to ensure consistent freezing at dierent
fill volumes.
Aramus bag assembly and RoSS shell

ARAMUS STANDARD BAG ASSEMBLIES
11User Manual | Rev. B 03/20 | Entegris, Inc.
SPECIFICATIONS
Aramus bag
size
1 L 2 L 5 L 10 L 20 L
Maximum
fill volume
0.9 L 1.7 L 3.5 L 6.5 L 11.0 L
RoSS shell
part number
SU-FS-0001-01 SU-FS-0002-01 SU-FS-0005-01 SU-FS-0010-01 SU-FS-0020-01
RoSS shell
weight
1.96 kg
(4.31 lbs)
2.83 kg
(6.23 lbs)
4.45 kg
(9.79 lbs)
6.13 kg
(13.49 lbs)
8.73 kg
(19.21 lbs)
RoSS shell
dimensions
208 mm ×
483 mm ×
37 mm
(8.2" × 13.7" × 1.5")
288 mm ×
533 mm ×
37 mm
(11.3" × 21" × 1.5")
358 mm ×
643 mm ×
52 mm
(14.1" × 25.3" × 2.0")
408 mm ×
703 mm ×
70 mm
(16" × 27.7" × 2.0")
503 mm x
883 mm ×
70 mm
(19.8" × 34.8" × 2.0")
Contact Entegris technical support if you have questions regarding this information, and to confirm you have the most up-to-date version.
WARNINGS
Aramus Bag Assembly
• Do not handle or carry the bags by the tubing
• Do not overfill or over pressurize the bag
• Regularly inspect for leaks and other damage
• Always ensure that all bag assembly components
are secure within the RoSS shell before use
• Do not use this product in a manner inconsistent
with its intended use
NOTE: User shall be solely responsible and liable for
validation and use of the Aramus bag assemblies beyond
the range of our specifications.

12
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
SHIPPING
Based on currently available information, the RoSS
shells may give user the best chance for successfully
shipping your product in an Aramus bag assembly.
However, since each company’s product likely has
unique freezing characteristics, the combined use
of RoSS shells and Aramus bag assemblies should
be fully validated by the customer.
As always, the customer is responsible for verifying fit
for use in combination with their product and in their
specific application.
INSPECTION
When unpacking the RoSS shell, be sure not to
scratch the stainless-steel shell cover when removing
the plastic overwrap. If a knife is used, keep the blade
point away from the stainless-steel shell cover.
Place the RoSS shell horizontally on a table making
sure the product label is oriented correctly.
ASSEMBLY INSTRUCTIONS
Step 1: Open RoSS Shell
Pull open the four, stainless-steel upper tabs; two
on the sides and two in the middle near the recessed
handle. Slide open the stainless-steel top lid until
completely removed.
Keep the lid fully removed and stored in a horizontal
position until step 5.
Step 2: Remove Foam Layers
Remove only the upper tubing foam and the rod foam
before placing the bag inside.
CAUTION! Safe foam pads along the sides
are attached to the RoSS shell and will be
damaged if they are removed.
Step 3: Fill and Insert Aramus Bag
Fill the Aramus bag to correct fill volume (see Specifi-
cations, page 11). It is suggested to fill to maximum
volume. If the bag is 10 L or greater, fill the bag while
it is inside the RoSS shell.
NOTE: The RoSS system will not work correctly if the
Aramus bag is positioned improperly.
Place the Aramus bag in the middle with the bag
chamber on the stainless-steel base, between the
side foam pads. The rod section needs to be placed
to the very back of the shell.
Wrap the tubing in a loose coil and place on top of
the lower tubing foam. Ensure the clamps on the
tubing are placed in a horizontal orientation to fit in
the shell and that the tubing and bag do not overlap.
Upper tabs
Lower foam,
DO NOT remove
Upper foam
Rod foam
Safe foam
Rod section

ARAMUS STANDARD BAG ASSEMBLIES
13User Manual | Rev. B 03/20 | Entegris, Inc.
Step 4: Secure Tubing
Place the upper foam and rod foam pads back in
their initial positions while making sure there are
no overhanging parts.
CAUTION! If the tubing and/or foam pads are
not placed in a snug, correct manner, they can
be damaged when closing the RoSS shell.
Step 5: Close the Shell
Insert and slide stainless-steel lid back onto the
shell. You may need to push down on the foam
while sliding the lid. Close the upper stainless-steel
tabs to secure the system.
Step 6: Insert Cryo Control Unit (CCU) Supports
CCUs are used to facilitate controlled freezing. When
freezing in standard static or blast freezers, insert CCU
onto the long sides of the RoSS shell, one at a time.
The CCU should fit snuggly with the shell.
NOTE: If using a plate freezer, CCUs are not needed with
the RoSS shell. Please refer to plate freezer manufacturers’
recommended procedures.
Step 7: Lift and Place in Freezer
(Static and Blast Freezing Only)
Lift by the recessed handles on each end and place
horizontally in a freezer. For consistent freezing,
ensure the RoSS shell bottom plate is not in direct
contact with the freezer shelf.
Always use the recessed handles for carrying the
RoSS shell and use a cart for the 20 L size.
Step 8: Thawing
Inspect the RoSS system to ensure no damage
or significant bulging occurred during freezing.
The stainless-steel tabs should all be secure, and
neither the bag nor tubing should be visible.
To thaw, store the RoSS system at ambient tempera-
ture on a bench or table. Thawing can be accelerated
using a fan.
Thawing can also be done in a plate freezer, refer to
manufacturer’s recommended procedures.
Rod foam
Upper foam
Handle
CCU supports

14
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
ORDERING INFORMATION
To order RoSS shells and CCUs for filling, freezing,
handling, and thawing applications, refer to the
following:
RoSS Shell
Part number Compatible Aramus bag assembly
SU-FS-0001-01 1 L
SU-FS-0002-01 2 L
SU-FS-0005-01 5 L
SU-FS-0010-01 10 L
SU-FS-0020-01 20 L
Cryo Control Unit (CCU)
Part number Compatible Aramus bag assembly
SU-CC-0001-01 1 L
SU-CC-0002-01 2 L
SU-CC-0005-01 5 L
SU-CC-0010-01 10 L
SU-CC-0020-01 20 L

ARAMUS STANDARD BAG ASSEMBLIES
15User Manual | Rev. B 03/20 | Entegris, Inc.
ARAMUS STANDARD BAG ASSEMBLY WITH CASSETTES
—
At lower volumes, the Aramus single-use bag
assemblies can be combined with cassettes to
provide greater protection during freezing and
handling. The following instructions explain how
to most eectively utilize the two together for fill-
ing, freezing, handling, and thawing applications.
Please use the correct Aramus bag size and cassette
combination. These cassettes are designed to be
used with standard pinch clamps allowing for sterile
connections. In general, do not use tubing lengths
longer than 12", which can be dicult to fit into the
cassette.
Fill the bags to greater than 25% of their maximum
fill volume stated in the specification table, page 16.
For example, 25% of a 250 mL bag's maximum fill
volume is 50 mL (200 x 0.25). To prevent adverse
freezing conditions, do NOT fill bags above their
maximum fill volume, or below 25% of this volume.
This information is for guidance purposes only.
Optimal fill volumes and ranges should be deter-
mined by the customer for each individual product,
freezing method, freezer type, and profile.
CAUTION: Failure to confirm fit for use under
the customer’s requirements may result in
product failure.
Aramus 50 mL bag assembly and cassette

16
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
SPECIFICATIONS
Aramus bag
size
20 mL 50 mL 100 mL 250 mL 500 mL
Maximum
fill volume
20 mL 50 mL 100 mL 200 mL 350 mL
Cassette
part number
SU-FS-0.05-C2 SU-FS-0.05-C2 SU-FS-0.10-C2 SU-FS-0.25-C2 SU-FS-0.50-C2
Cassette
dimensions
203 mm x
160 mm x
21 mm
(8.0" x 6.3" x 0.8")
203 mm x
160 mm x
21 mm
(8.0" x 6.3" x 0.8")
213 mm x
161 mm x
21 mm
(8.4" x 6.3" x 0.8")
302 mm x
157 mm x
22 mm
(11.9" x 6.2" x 0.9")
305 mm x
190 mm x
23 mm
(12.0" x 7.5" x 0.91")
Contact Entegris technical support if you have questions regarding this information, and to confirm you have the most up-to-date version.
WARNINGS
Aramus Bag Assembly
• Do not handle or carry the bags by the tubing
• Do not overfill or over pressurize the bag
• Regularly inspect for leaks and other damage
• Always ensure that all bag assembly components
are secure within the cassette before usage
• Do not use this product in a manner inconsistent
with its intended use
NOTE: User shall be solely responsible and liable for
validation and use of the Aramus bag assemblies beyond
the range of our specifications.
Cassette
• Always wear appropriate personal protective
equipment including gloves when handling
cassette
• Use two hands when transporting frozen cassettes
• Do not disassemble cassette before use
• Though reusable, inspect cassette prior to each use
• To prevent damage to the product, do not
mishandle the cassette
Note: User shall be solely responsible and
liable for validation and use of the Aramus
bag assemblies and cassettes beyond the
range of our specifications.

ARAMUS STANDARD BAG ASSEMBLIES
17User Manual | Rev. B 03/20 | Entegris, Inc.
SHIPPING
Based on currently available information, the cas-
settes may give user the best chance for successfully
shipping your product in an Aramus bag assembly.
However, since each company’s product likely has
unique freezing characteristics, the combined use of
cassettes and Aramus bag assemblies should be fully
validated by the customer.
As always, the customer is responsible for verifying fit
for use in combination with their product and in their
specific application.
INSPECTION
When unpacking the cassette, be sure to check for
damage such as dents or missing components. Also,
open and close the cassette to ensure functionality.
ASSEMBLY INSTRUCTIONS
Step 1: Open Cassette
Swivel open the locking bar and then lift the
tab vertically.
Step 2: Fill and Insert Aramus Bag Assembly
Place filled, Aramus bag into cassette. Orient any
connected tubing, clamps, and connectors as shown
to fit into the cassette. Ensure no components are
raised above the cassette’s side walls.
Tab
Locking bar

18
ARAMUS STANDARD BAG ASSEMBLIES
User Manual | Rev. B 03/20 | Entegris, Inc.
Step 3: Close Cassette
Close tab and then swivel locking bar into a closed
position. There should be little to no resistance from
the bag when closing. Ensure the cassette does not
bulge once closed.
NOTE: If the cassette bulges when closed, go back to
step 2 and reposition the components.
Step 4: Freezing and Storage
For ultra-low temperatures, the cassette assembly
can be placed directly into static and blast freezers
in a horizontal orientation (flat on shelf).
For freezing in a liquid nitrogen Dewar, a rack is
recommended. Be sure to slide the cassette com-
pletely inside the rack, and lock if applicable, before
immersing in liquid nitrogen vapor.
CAUTION! Always use cryogenic gloves when
handling cassettes or racks in low temperatures.
Step 5: Thawing
Inspect the cassette assembly to ensure no damage
or significant bulging occurred during freezing. The
locking bar and tab should be secure.
To thaw, remove the bag from the cassette assem-
bly and store at ambient temperature on a bench
or table. Other methods are available to accelerate
thawing such as using a fan or immersing the bag
in a water bath.
Locking bar

ARAMUS STANDARD BAG ASSEMBLIES
19User Manual | Rev. B 03/20 | Entegris, Inc.
ORDERING INFORMATION
To order cassettes for filling, freezing, handling, and
thawing applications, refer to the following:
Cassette
Part number Compatible Aramus bag assembly
SU-FS-0.05-C2 20 mL, 50 mL
SU-FS-0.10-C2 100 mL
SU-FS-0.25-C2 250 mL
SU-FS-0.50-C2 500 mL
ROSS SHELL AND CASSETTE DISCLAIMER
—
The items identified above as Special Products are
experimental prototype products. Special Products
have not been subjected to the testing, validation and
quality assurance procedures normally implemented
by Entegris pursuant to the process of commercial
introduction of standard Entegris Products. As a re-
sult, Entegris is unable to oer purchasers of Special
Products the same assurance as to the operating
capabilities and appropriate applications use that it
provides with respect to standard Entegris Products.
Entegris makes no representation as to such matters
or as to the physical characteristics or operating spec-
ifications of Special Products. Special Products are
sold to those customers willing to purchase them
on the terms of this Special Products Quotation as
an accommodation and are further subject to the
following terms.
Customer acknowledges that:
a) The Special Products may have defects or deficien-
cies in design, manufacture and/or materials, and
Entegris can provide no assurance that the Special
Products will operate uninterrupted or error free.
b) Entegris can provide no assurance that it will be
able to support the Special Products after sale, that it
will commercially introduce the Special Products, that
it will not make significant changes to future versions
of the Special Products or that the Special Products
will be available in the future.
c) Entegris can provide no assurance that the use
of the Special Products or any information relating
thereto or contained therein will not infringe any
patent, copyright, trade secret, or trademark of any
third party.
Furthermore, Special Products are sold WITH NO
WARRANTY on an “AS IS" and “WITH ALL FAULTS"
basis. ENTEGRIS MAKES NO OTHER WARRANTY,
EXPRESSED OR IMPLIED. THERE IS NO WARRANTY
OF MERCHANTABILITY OR FITNESS FOR A PARTIC-
ULAR PURPOSE. AT THIS TIME, ENTEGRIS CANNOT
WARRANTY SPECIAL PRODUCTS FOR STERILITY,
SHIPPING, OR SHELF LIFE. IT IS THE USERS’ RESPON-
SIBILITY TO ENSURE PRODUCT SUITABILITY FOR
USE WITHIN THEIR APPLICATION