GeneAmp PCR System 2400 Use and care manual

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GeneAmpâPCR System 2400
Coolant Replacement Procedure
The GeneAmpâPCR System 2400 Thermal Cycler requires routine maintenance which
can be performed by the end user. This routine maintenance will improve the
performance of the instrument and may prevent more costly in-house repairs. The
procedure described here is for the periodic checking of the coolant in the instrument
and the procedure for re-filling the coolant, should the need arise.
Applied Biosystems service personnel can perform this procedure should you be
reluctant to do so. This service requires the instrument to be shipped to our repair
facility and the user would be charged all fees associated with this procedure. To
request a service repair of your instrument please call our Service Call Center at 1-800-
831-6844.
Applied Biosystems is not responsible for any damage to the machine, or its parts,
during the performance of this procedure.

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GeneAmpâPCR System 2400
Coolant Service Procedure
Instructions for checking the coolant level, cleaning the coolant
reservoir filter, and re-filling the coolant reservoir for the
GeneAmpâPCR System 2400 thermal cycler.

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Step 1. Preparing the instrument:
Turn off and unplug the instrument. Unscrew and remove the four screws located on the
sides of the instrument. Two screws are on the right side and two screws are on the left
side.
Step 2. Opening the instrument cover:
Carefully remove the cover of the instrument by lifting straight up.
NOTE: Use caution when lifting the cover. A ribbon cable is connected to the lid and the
base of the instrument. Damage to the cable can result in the costly replacement of
parts.

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After the cover has cleared the instrument base, start to bring the cover to the side.
The ribbon cable is long enough that the cover can be placed on the benchtop next to
the instrument.

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Step 3. Locating the coolant reservoir:
After the cover is removed locate the coolant reservoir. The coolant reservoir is located
in the back left-hand corner of the instrument.

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Step 4. Opening the coolant reservoir:
Unscrew the coolant reservoir lid.
Check the coolant level – the coolant reservoir should be full with coolant. If the coolant
level is low please continue to step 5. If the coolant reservoir is full, please skip to step
6.

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Step 5. Filling the coolant reservoir:
If the coolant level is low, prepare approximately 700 ml of 50% distilled water and 50%
Prestoneâ5/150 Extended Life Antifreeze. Pour the coolant (distilled water/antifreeze
mixture) into the coolant reservoir until the reservoir is full. Properly dispose of the
excess coolant.
Step 6. Cleaning the coolant reservoir filter:
Pull out the basket filter located inside the coolant reservoir. Clean the filter with a scrub
brush and distilled water. After cleaning, place the filter back in the coolant reservoir and
screw on the coolant reservoir.

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Step 7. Re-Assembling the Instrument:
When finished with previous steps, carefully place the cover back on- Please be careful
not to crimp the ribbon cable. Replace the 4 screws removed in Step 1.
For Research Use Only. Not for use in diagnostic procedures.
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the U. S. and
certain other countries.
GeneAmp is a registered trademark of Roche Molecular Systems, Inc.
All other trademarks are the sole property of their respective owners.