GeneReach Taco 24 User manual

2014/03
Research Use Only
Nucleic AcidAutomatic Extraction System
User Manual
Manufacturer:
GeneReach Biotechnology Corporation
TEL: [phone] Email: [email protected]
No. 19, Keyuan 2nd Road, Central Taiwan Science Park, Taichung City 407, Taiwan
Website: www.tacomag.com
Cat. atcd-02
24

i
Content
Symbols...............................................................................................................................................1
1. Introduction....................................................................................................................................2
1.1 Intended Use ..........................................................................................................................2
1.2 taco™24 Extraction Principle ..............................................................................................2
2. taco™ 24 Layout............................................................................................................................3
2.1 Product Components..............................................................................................................3
2.2 taco™ 24 Specifications........................................................................................................3
2.3 Equipments to Be Supplied by User......................................................................................3
2.4 Front View of taco™24........................................................................................................4
2.5 Rear View of taco™24.........................................................................................................6
2.6 Loading taco™ Plate & Comb ..............................................................................................7
3. Important Notes.............................................................................................................................9
3.1 Safety Information.................................................................................................................9
3.2 Emergency Stop.....................................................................................................................9
4. Operation Procedure of taco™ 24..............................................................................................10
4.1 How to Start taco™ 24 ........................................................................................................10
4.2 How to Paue taco™ 24........................................................................................................10
4.3 How to Reset taco™ 24.......................................................................................................11
4.4 When and How to Turn on UV Lamp..................................................................................11
4.5 How to Shut Down taco™ 24..............................................................................................11
5. Cleaning and Maintenance…………………………….……………………………………….12
5.1 General Care……………………………………………………………………….………12
5.2 Dealing with Spills...............................................................................................................12
6. Troubleshooting............................................................................................................................13

taco™ 24 Nucleic Acid Automatic Extraction System
1
Symbols
Date of manufacturing
Manufacturer
Catalog number
Serial number
Alternative current
Consult instruction for use
Caution, consult accompanying documents
WEEE symbol
This product is required to comply with the European
Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96/EC. Please follow your institutional
requirements for disposal of the accessories.

taco™ 24 Nucleic Acid Automatic Extraction System
2
1. Introduction
The taco™ 24 Nucleic Acid Automatic Extraction System (taco™ 24) is a magnetic
bead-based DNA extraction and purification device. Nucleic acids bind to silica-coated magnetic
beads, which can be separated easily from biochemical contaminants in the solution through
automatic transfer and processing of magnetic beads. Purified nucleic acids could be eluted from
the beads after several washing steps.
1.1 Intended Use
taco™ 24 is developed to work with nucleic acid extraction kits developed by GeneReach
Biotechnology Corporation (GeneReach) to extract nucleic acids from samples for research
use purpose.
This product is intended for professional use by properly-trained laboratory technicians
familiar with molecular biology techniques.
1.2 taco™ 24 Extraction Principle
taco™ 24 is based on the magnetic separation technology to work with the specially designed
disposable taco™ Plate & Comb which are provided with all reagent kits.
Samples and reagents, including the magnetic particles, are dispensed into wells of the
extraction plate manually according to instructions of the kit before extraction starts. The
automated steps performed by taco™ 24 include:
Transfer of magnetic beads and mixing magnetic beads with samples.
Sample lysis.
Binding of nucleic acids to magnetic beads.
Washing steps to remove biochemical other than nucleic acids.
Elution of nucleic acids from magnetic beads.

taco™ 24 Nucleic Acid Automatic Extraction System
3
2. taco™ 24 Layout
2.1 Product Components
2.2 taco™ 24 Specifications
2.3 Equipments to Be Supplied by User
taco™Nucleic Acid Extraction Kit
taco™Plate & Comb
Uninterrupted power system (UPS) (optional)
Laminar air-flow (optional)
Disposable gloves
Protective goggles
Lab coat
Note: To avoid power failure, connecting taco™ 24 to an UPS is recommended.
Component
Quantity
taco™ 24
1
Power Cord
1
User Manual
1
Warranty Card
1
Specification
Dimensions (W ×D ×H)
42 cm ×30 cm ×38 cm
Net Weight
23.4 kg
Power Requirement
100-120V/ 200-240VAC, 50/60 Hz, 2A
Fuse
F2A/250V
Operating Temperature
16–30°C
Throughput
24 samples
Extraction time
Within 1 hour

taco™ 24 Nucleic Acid Automatic Extraction System
4
2.4 Front View of taco™ 24
2.4.1 LED Light Signals
No.
Indicator
*LED light Color
Signal
Description
1
Run/Stand-by signal
*Green
Lights on
Self-test passed. Ready to run.
Lights flashing
Extraction in progress.
2
Pause/End signal
*Yellow
Lights on
Extraction finished.
Lights flashing
Extraction paused.
3
UV On signal
*Green
Lights on
UV lamp on.
4
Error signal
*Red
Lights on
Any error occurred.
④
②
①
③

taco™ 24 Nucleic Acid Automatic Extraction System
5
2.4.2 Operation Buttons
No.
Button
Description
Function disabled under the
following status
1
Door
Press to open/close the door.
1. During the extraction.
2. UV lamp is on.
2
Pause/Reset
Short press to pause the
machine.
1. During magnetic bead transfer.
2. During magnetic bead drying.
3. UV lamp is on.
While paused or extraction
finished, long press to reset the
machine.
Reset is functional only when
extraction is paused or finished.
3
Start
Press to start/resume the
machine.
1. Door is open.
2. UV lamp is on.
②
③
①
Door
Pause/Reset
Start

taco™ 24 Nucleic Acid Automatic Extraction System
6
2.5 Rear View of taco™ 24
No.
Item
Description
1
Cooling vents
Ventilation
2
UV lamp switch
To turn on/off the UV light
3
Main switch
To turn on/off the main power
4
Socket
To connect to the power cord
④
②
①
③

taco™ 24 Nucleic Acid Automatic Extraction System
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2.6 Loading taco™ Plate & Comb
2.6.1 Loading the 48-Well Extraction Plate
No.
Item
Description
1
Magnetic rod module
Shipping anchor of the magnetic rod.
2
Lower rack
Housing for the Mixing Comb.
3
Mixing Comb
Protection of magnetic rods and mixing of sample with
magnetic bead.
4
48-Well Extraction Plate
Deep-well plate for extraction. Use one Mixing Comb
for each 48-Well Extraction Plate when processing
extraction.
5
Notched corner of the
48-Well Extraction Plate
The 48-Well Extraction Plate should be fully inserted into
the plate holder with the notched corner pointing to far
right.
6
Plate holder
Housing for the 48-Well Extraction Plate.
Note:1The Mixing Comb and the 48-Well Extraction Plate should be placed in the same slot
when processing extraction (See illustrations above).
2Inadequate installation of taco™ Plate & Comb may damage the machine.
①
②
③
④
⑤
⑥

taco™ 24 Nucleic Acid Automatic Extraction System
8
2.6.2 Loading the Mixing Comb
2.6.3 Releasing the Mixing Comb
Note: Use of non-recommended consumables may affect performance of the device.
No.
Item
Description
1
Tenon of Mixing Comb
Slide the Mixing Comb into position. Make
sure the tenon on the Mixing Comb is inserted
fully into the mortise on the lower rack.
No.
Item
Description
1
Grip of Mixing Comb
Press the grip of Mixing Comb to release.
①Tenon
Mortise
①Grip