GL Sciences GL-Tip SDB User manual

GL-Tip™ SDB Instruction Manual
Thank you for purchasing GL-Tip™ SDB. GL-Tip SDB is a sample preparation tip column packed with styrene di vinyl
benzene polymer designed for desalting and enriching peptide samples, particularly hydrophobic phosphopeptides.
GL-Tip™ SDB is often used in combination with GL-Tip™ GC (available separately, P/N 7820-11201) to ensure that
peptides with the widest range of polarity are captured in high yields.
1. Product Description
○ Check for damage or missing parts.
Contents
Part No. (Order No.)
7820-11200
GL-Tip SDB
96 tips
Instruction Manual
1
2. Product Handling
○ Handle GL-Tips delicately; avoid dropping or tapping the tips, which may harm the column bed.
○ Do not autoclave GL-Tips
○ GL-Tips are designed for use with a centrifuge. Attempting to use GL-Tips with syringes, pipettes, or
any air-forced solvent flow methods may result in reduced or irreproducible results.
○ Actual maximum or minimum sample concentration and loading volume are sample-dependent, and can vary with
different detergents or denaturants present in the sample. Therefore, sample loading conditions optimum –for –your
–particular -sample must be determined empirically in your laboratory.
○ Make sure to use the purest acetonitrile available. Even some HPLC-grade acetonitrile has been shown to contain
both high and low MW impurities which could complicate MS results.
○ GL-Tip SDB are disposable and should not be reused.
○ GL-Tip SDB should be stored in an environment with relatively constant temperature and humidity.
3. Solvent / Solution Preparation*
①Recommended Chemicals
・Highly pure acetonitrile (HPLC grade or higher)
・Trifluoroacetic acid (HPLC grade or higher)
②Solutions Required
・Solution A: Water (0.1% TFA) or 5% acetonitrile aqueous solution (0.1% TFA)
・Solution B: 80% acetonitrile aqueous solution(0.1%TFA)
*Note: Prepare solutions A & B on the day of the experiment
Japan: 6-22-1 Nishishinjuku, Shinjuku, Tokyo, 163-1130, Japan Tel. [phone], Fax. [phone] www.glsciences.com email: [e-mail]
USA: 4733 Torrance Blvd, Suite 255, Torrance, CA 90503 Tel. [phone], FAX [phone], www.glsciencesinc.com email: [e-mail]

4. Purification Protocol
Make sure that the solution is completely eluted from the tip column after each centrifuge operation.
(**RT: Room Temperature)
- Connect a centrifuge adaptor to a waste fluid tube and insert
GL-Tip SDB column (Fig. 1)
- Add 20 uL of Solution B and centrifuge (3,000 x g, 2min, RT**)
- Add 20 uL of Solution Aand centrifuge (3,000 x g, 2min, RT)
- Add a sample to the tip column
- Centrifuge (3,000 x g, 5min, RT) Higher centrifugal speeds may lead to poor recovery.
- Apply extra centrifuge if liquid output is not sufficient.
- Add 20 uL of Solution A and centrifuge (3,000 x g, 2min, RT)
-
- Put the Tip Column into a recovery tube
- Add at least 50 uL of Solution B and centrifuge (3,000 x g, 3min, RT）
GL-Tip SDB is manufactured, inspected, packed and shipped under strict standards of quality control. Please contact
us if you have questions regarding the proper use and performance of GL-Tips.
GL-Tips are intended for research purposes and no guarantees or warrantees are expressed or implied beyond what is
described herein.
The following GL-Tip adaptors are used to secure the tips in the collection tubes or 96-well plates.
・Part No.: 5010-21514: Centrifugal adaptor (24 units)
・Part No.: 5010-21341: Centrifugal adaptor 200μL for 96 well plate (1 unit)
・Part No.: 5010-21343: Centrifugal adaptor 200μL for 96 well plate (2 units)
Japan: 6-22-1 Nishishinjuku, Shinjuku, Tokyo, 163-1130, Japan Tel. [phone], Fax. [phone] www.glsciences.com email: [e-mail]
USA: 4733 Torrance Blvd, Suite 255, Torrance, CA 90503 Tel. [phone], FAX [phone], www.glsciencesinc.com email: [e-mail]
ENG_1-1
Tip Column
Conditioning
Equilibration
Adsorption
Rinsing
Elution
Peptides
Centrifugal adapter
Waste Fluid Tube
Fig.1 Setting of Tip Column
Tip Column
Centrifugal adapter
Waste Fluid Tube
Fig.1 Setting of Tip Column
Tip Column