Globe 3375 User manual

3375
Manual Pipet Controller
User Manual
What’s in the box:
• Manual Pipet Controller
• User Manual
PRODUCT DESCRIPTION
The Globe 3375 Manual Pipette Controller
may be used with glass or plastic pipettes
to facilitate the pipetting of a wide range of
liquids. Pipettes within the volume range
of 0.1ml to 100ml can be used. When the
controller is correctly used, the liquid
comes into contact with the pipette only.
COMPONENTS
Blow-Out
Aspirate & Dispense Lever
Housing
Silicone
Nose
Filter
Locking Ring
Suction Bellows
See reverse for pipetting instructions . . .

3375
Manual Pipet Controller
User Manual
PIPETTING
1. Fit the pipette
Gently insert the pipette into the silicone adapter inside the
nose cone. Do not use excessive force as this may damage
the silicone insert.
2. Fill the Pipette
Hold the controller in a vertical position with the pipette tip
down and squeeze the suction bellows. Place the tip of the
pipette into the liquid to be aspirated. Press the aspirate
lever slowly upwards allowing the liquid to ll the pipette
until the meniscus is slightly above the desired volume mark.
Note: Do not allow liquid to enter the controller. The function
of the lter will be severely impaired reducing suction efciency.
(Spare lters are available; Globe item #3375-FIL)
The further up the lever is pressed, the stronger the suction
becomes and the faster the meniscus rises.
In the case of large volume pipettes, the vacuum contained
in the suction bellows is not sufcient to draw in all the
liquid at once. Therefore, press the suction bellows again to
continue drawing up liquid.
3. Adjust the Meniscus
Wipe the pipette tip with a suitable lint-free tissue. Press the
dispense lever down slowly until the required meniscus has
been reached.
4. Dispense the Pipette
Hold the collection vessel in an inclined (diagonal) position,
placing the pipette tip against the collecting vessel’s wall.
Press the dispense lever down to empty the pipette.
5. ‘Blow-Out’ Pipettes
As soon as the meniscus in the pipette comes to a standstill,
press the blow-out bellows to expel the last few drops. To
ensure complete dispensing, wipe the pipette tip upwards a
few millimeters along the inside wall of the collecting vessel.
®
Customer Service, Sales and Technical Support:
Phone: [phone] • 1 [phone]
Fax: [phone] • E-mail: mail@globescientic.com
© 2019 Globe Scientic Inc. All rights reserved. The Globe Scientic logo is a registered trademark of Globe Scientic Inc. 3375 Manual 19089
www.globescientic.com
SERVICE:
If service is required beyond these instructions, please contact
Globe Scientic.
6. ‘To Deliver’ Pipettes
As soon as the liquid in the pipette comes to a standstill, wait
for the period of time required to ensure the timed liquid has
been fully dispensed.
7. To Remove Pipette
With the controller in a vertical position in one hand, hold
the pipette at its upper end with your opposing hand and
gently twist to remove it from the silicone adapter.
8. Replace Filter and Clean the Aadapter
If the pipette has been overlled or the suction capacity is
impaired, proceed as described below:
a. Remove the pipette
b. Unscrew the nose cone
c. Remove the silicone adapter from the housing by
pulling it upwards. Use a wash bottle to rinse the adapter.
Blow out the liquid and allow to dry completely. If any
damage is seen on the adaptor, replace with a new one.
d. Remove the lter and replace with a new one.
e. Re-assemble in the reverse order of the above
instructions.
9. Leak Test
Attach a pipette and aspirate liquid to a minimum of half
the capacity of the pipette. Hold the pipette over a suitable
reservoir. If liquid drips from the pipette tip, dismantle the
unit (see step 8) and re-assemble correctly.