Harvard Apparatus Pump 11 Elite OEM User manual

Harvard Apparatus Pump 11 Elite OEM Module Series
Publication 5420-007 Rev B
www.harvardapparatus.com
phone [phone]
email [email protected]
Pump 11 Elite OEM
Module Series
USER’S MANUAL

2
Table of Contents
SUBJECT PAGE NO.
Table of Contents
General Information........................................................................................................4
Safety Information .................................................................................................4
Warranty and Repair Information...........................................................................5
Manual Description.....................................................................................5
Warranty .....................................................................................................5
Repair Facilities and Parts...........................................................................5
Serial Numbers ...........................................................................................5
Calibrations .................................................................................................5
Specifications.........................................................................................................6
Product Overview.............................................................................................................8
Pump Models ........................................................................................................8
Operating Instructions ...........................................................................................9
Power Connections ...............................................................................................9
Syringe Loading ...................................................................................................10
External Pump Control.........................................................................................11
Setting up HyperTerminal .........................................................................11
Pump Chain Commands ......................................................................................12
Using the Pump 11 Elite Series Pump Chain Commands ........................12
Error Messages ........................................................................................13
Command Errors.......................................................................................13
Argument Errors .......................................................................................13
System Commands ..................................................................................13
Run Commands ........................................................................................15
Rate Commands .......................................................................................15
Volume Commands ..................................................................................16
Time Commands ......................................................................................17
Digital I/O Commands...............................................................................18
Internal Commands ..................................................................................18
Serial Command Notes.............................................................................18
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3
Table of Contents
Appendices....................................................................................................................... 19
Appendix A: Syringe Volume/Diameter Reference Table ....................................19
Appendix B: Min/Max Flow Rates .......................................................................20
Appendix C: External Connections ......................................................................22
User I/O Connector Specifications............................................................22
Utilizing USB Interface..............................................................................23
RS-485 ......................................................................................................25
Legacy RJ-11 Option ................................................................................26
Appendix D: Maintenance ...................................................................................27
Maintenance .............................................................................................27
Upgrading Pump 11 Elite Series Software ...............................................28
Troubleshooting ........................................................................................29
Appendix E: Ordering Information .......................................................................30
SUBJECT PAGE NO.
Pump 11 Elite OEM Module USER’S MANUAL
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4
Table of Contents
General Information
SAFETY INFORMATION
Please read the following safety precautions to ensure proper use of your syringe pump. If the equipment is used in a
manner not specified, the protection provided by the equipment may be impaired.
To Prevent Hazard or Injury:
USE PROPER POWER SUPPLY
The pump is supplied with an approved power supply and line cord. To maintain the safety integrity of the device, use
only the following power supplies:
Cui Inc. Globtek Inc.
Model: 3A – 242DB24 Model: GT–41062–T3
Output: 24V – 1.0A Output: 24V – 0.75A
Input: 100–240V~50–60 Hz 0.8A Input: 100–240V~50–60 Hz 0.6A
USE PROPER LINE CORD
Use only the specified line cord for this product and make sure line cord is certified for country of use. The operating
voltage range for the Pump 11 Elite Series is 12-30 VDC. The universal power supply operating voltage range is
100-240 VAC, 50-60 Hz.
GROUND THE PRODUCT
This product is grounded through the grounding conductor of the power cord. To avoid electric shock, the grounding
conductor must be connected to earth ground. Before making any connections to the input or output terminals of the
product, ensure that the product is properly grounded.
MAKE PROPER CONNECTIONS
Make sure all connections are made properly and securely. Any signal wire connections to the unit must be no longer than 3
meters.
OBSERVE ALL TERMINAL RATINGS
Review the operating manual to learn the ratings on all connections.
AVOID EXPOSED CIRCUITRY
Do not touch any electronic circuitry inside of the product.
AVOID PINCH HAZARD
A pinch hazard may exist between the pusher block and end blocks. Avoid placing fingers between these points while
the pump is running.
DO NOT OPERATE WITH SUSPECTED FAILURES
If damage is suspected on or to the product do not operate the product. Contact qualified service personnel to perform
inspection.
ORIENT THE EQUIPMENT PROPERLY
Do not orient the equipment so that it is difficult to operate the disconnection device.
PLACE PRODUCT IN PROPER ENVIRONMENT
Review the operating manual for guidelines for proper operating environments.
OBSERVE ALL WARNING LABELS ON PRODUCT
Read all labels on product to ensure proper usage.
Protective
Ground Terminal
CAUTION
Refer to Manual
!
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5
General Information
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WARRANTY AND REPAIR INFORMATION
Manual Description
This manual is designed to provide all operational and Method information required to operate and maintain the
Pump 11 Elite Syringe Pumps. The functions and features are described in the Technical Specifications section.
Warranty
Harvard Apparatus warranties this instrument for a period of two years from date of purchase. At its option,
Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials.
This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, or
accident. This warranty extends only to the original consumer purchaser.
IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL
DAMAGES. Some states do not allow the exclusion or limitation of incidental or consequential damages
so the above limitation or exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF
MERCHANTABILITY, OR FITNESS FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states
do not allow this limitation on an implied warranty, so the above limitation may not apply to you.
If a defect arises within the warranty period, promptly contact Harvard Apparatus, 84 October Hill Road,
Holliston, Massachusetts 01746 using out toll free number 1-800-272-2775 or outside the US call 1–508-893-
8999. Email address is [email protected]. Goods will not be accepted for return unless an RMA
(returned materials authorization) number has been issued by our customer service department. The customer is
responsible for shipping charges for non-warranty repairs. Please allow a reasonable period of time for completion
of repairs or replacement. If the unit is replaced, the replacement unit is covered only for the remainder of the
original warranty period dating from the purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary from state to state.
Repair Facilities and Parts
Harvard Apparatus stocks replacement and repair parts. When ordering, please describe parts as completely as
possible, preferably using a part number obtained from our Technical Support department. If practical, enclose a
sample part or sketch. We offer a complete reconditioning service.
Serial Numbers
All inquiries concerning our product should refer to the serial number of the unit, located on the rear panel.
Calibrations
All electrical apparatus are calibrated at rated voltage and frequency. While the flow and volume will stay calibrated,
the peak pressure may vary. Harvard Apparatus recommends an annual calibration of the pump.
Pump 11 Elite OEM Module USER’S MANUAL
!CAUTION: FOR RESEARCH USE ONLY. NOT FOR CLINICAL USE ON
PATIENTS.
!CAUTION: REFER TO SAFETY INFORMATION AND SETTING UP THE
HARVARD APPARATUS PUMP 11 ELITE SERIES BEFORE PLUGGING
IN THE PUMP.

6
Pump 11 Elite OEM Module USER’S MANUAL
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General Information
Accuracy ± 0.5% ± 0.35%
Reproducibility ± 0.05% ± 0.05%
Syringes (Min./Max.) 0.5 µl / 60 ml (10 ml dual) 0.5 µl / 10 ml
Flow Rate:
Minimum (0.5 µl syringe) 1.26 pl/min 0.54 pl/min
Maximum (10 ml syringe) 26.02 ml/min 11.70 ml/min
Maximum (60 ml syringe) 88.40 ml/min N/A
Display None None
Non-Volatile Memory Stores all settings Stores all settings
Connectors:
RS-485 IEEE-1394, 6 pos IEEE-1394, 6 pos
USB Type B Type B
I/O & TTL 15 pin D-Sub Connector 15 pin D-Sub Connector
Footswitch Mini phono jack Mini phono jack
RS-232 (option) RJ-11 RJ-11
Linear Force (Max) 16 kg (35 lbs) @ 100% Force Selection 16 kg (35 lbs) @ 100% Force Selection
Drive Motor 0.9° Stepper Motor 0.9° Stepper Motor
Motor Drive Control Microprocessor with 1/16 microstepping Microprocessor with 1/16 microstepping
Number of Microsteps
per one rev. of Lead Screw
15,360 20,480
Step Resolution 0.069 µm/µstep 0.031 µm/µstep
Step Rate:
Minimum 27.5 sec/µstep 27.5 sec/µstep
Maximum 26 µsec/µstep 26 µsec/µstep
Pusher Travel Rate:
Minimum 0.15 µm/min 0.068 µm/min
Maximum 159.00 mm/min 71.55 mm/min
Input Power 12-30 VDC (24V recommended) 12-30 VDC (24V recommended)
Input Power Connection 2.5mm ID x 5.5mm OD male plug 2.5mm ID x 5.5mm OD male plug
Power Supply 100-240 VAC, 50-60 Hz, 8 Watts Universal Power
Supply, Use Only a Harvard Apparatus Approved
Power Supply and Line Cord
100-240 VAC, 50-60 Hz, 8 Watts Universal Power
Supply, Use Only a Harvard Apparatus Approved
Power Supply and Line Cord
Dimensions 24.13 x 17.15 x 10.67 cm (9.5 x 6.75 x 4.2 in) 24.13 x 17.15 x 10.67 cm (9.5 x 6.75 x 4.2 in)
Weight 2.3 kg (5.08 lbs) 2.3 kg (5.08 lbs)
Specications Pump 11 Elite OEM Pico Plus Elite OEM

7
General Information
Pump 11 Elite OEM Module USER’S MANUAL
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Atmospheric Specifications:
Operating Temperature 4°C to 40°C (40°F to 104°F) 4°C to 40°C (40°F to 104°F)
Storage Temperature -10°C to 70°C (14°F to 158°F) -10°C to 70°C (14°F to 158°F)
Operating Humidity See Chart Below See Chart Below
Storage Humidity 20% to 80% RH, non condensing 20% to 80% RH, non condensing
Method of Operation Continuous Continuous
Classification Class I Class I
Pollution Degree 1 1
Installation Category II II
Supplier Name Harvard Apparatus Harvard Apparatus
Supplier Address 84 October Hill Rd., Holliston, MA 01746 84 October Hill Rd., Holliston, MA 01746
Supplier Phone Number [phone] [phone]
Regulatory Certifications CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme
Safety Declarations ANSI/UL 61010-1 ED. 3 ; CAN/CSA C22.2 No.
61010-1Ed 3 ; IEC 61010-1 Ed 3 ; CENELEC EN
61010-1 ; CB Scheme
ANSI/UL 61010-1 ED. 3 ; CAN/CSA C22.2 No.
61010-1Ed 3 ; IEC 61010-1 Ed 3 ; CENELEC EN
61010-1 ; CB Scheme
EMC Declaration FCC 47CFR 15B Class A; EN61326-1 FCC 47CFR 15B Class A; EN61326-1
Specifications Pump 11 Elite OEM Pico Plus Elite OEM

8
Pump 11 Elite OEM Module USER’S MANUAL
Product Overview
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Product Overview
The Harvard Apparatus Pump 11 Elite Series is
the next generation of syringe pumps.
A green led light on the front panel makes it easy to see
if the pump is running.
The pumps are versatile and can be interconnected
through the RS-485 interface. Pump 11 Elite Series
models can be mixed and matched in the daisy chain
offering maximum flexibility. Up to 99 pumps can be
linked together through the RS-485 interface provided
each pump is assigned its own unique pump address.
Flow performance is optimized with a small step angle
stepping motor that drives a precision lead screw
and pusher block. The syringe mechanism is easy to
use and securely holds the syringes for smooth flow
performance.
Advanced microstepping techniques are employed
to further reduce the step angle to eliminate flow
pulsation. The accuracy is 0.5% (0.35% for Pico Plus
Elite). A wide dynamic flow range from 1.26 pl/min to
88.40 ml/min (0.54 pl/min to 11.70 ml/min for the Pico
Plus Elite) can be programmed into the pump. Flow
rates are selectable with user selected engineering
units from ml, µl, nl, pl, and hours, minutes and
seconds.
Up to 16 kg (35 lbs) of linear force is available. This force
is user adjustable to ensure the right force is applied for
the various syringe sizes.
Easy external connections to a computer or other
control devices are made through USB interface. Simple
ASCII commands make communication with the pump
easy. For direct control of the pump, the user can use
the I/O interface (15pin Dsub). One trigger output and
a trigger input in for external events such as a process
parameter are available. The footswitch input will allow
the control of the pump through an external device.
The unit also has an output for run indication allowing
connection to a remote LED.
The 11 Elite OEM Pumps are available with single and
dual syringe options. Complete flexibility is offered with
the ability to use most manufacturers syringes ranging
from 0.5 µl to 60 ml single syringe and 5 µl to 10 ml dual
syringe.
Pump Models
Single Syringe Infuse/Withdraw Programmable
Dual Syringe Infuse/Withdraw Programmable
Pico Plus (dual) Infuse/Withdraw
Programmable

9
Pump 11 Elite OEM Module USER’S MANUAL
Product Overview
OPERATING INSTRUCTIONS
POWER CONNECTIONS
1. Plug the power cord in. The Pump 11 Elite Series has a DC power input connector on the rear of the unit. The
operating range for the Pump 11 Elite Series series is 12-30 Vdc.
2. Turn on the main power switch.
!CAUTION: DO NOT CONNECT TO
FIREWIRE PORTS ON A COMPUTER.
DAMAGE MAY OCCUR TO THE
PUMP OR COMPUTER.
I/O Connector
Foot Switch
Main Switch
DC Power Input
*Power Source -
When powering
a pump in a chain
through an RS-
485 cable, toggle
switch to RS-485.
Only one pump can
be powered from
master through
RS-485
RS-485 Connection
(Pump to Pump)
USB Serial Input
(Type B)
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Best performance guaranteed when using
24V power supply.

10
Pump 11 Elite OEM Module USER’S MANUAL
Product Overview
until the syringe barrel clamp (10) is tight
against the syringe barrel. Do not overtighten,
especially when using glass syringes.
6. Secure the syringe flanges by pushing the
retaining brackets (4,5) tightly against the
syringe flanges using the bracket clamping
knobs (7,8). The syringe plunger should be
secured by the pusher block retaining bracket
and the flange of the syringe should be
secured by the syringe holder block retaining
bracket.
7. Tighten the mechanical stop collars (9) using
allen wrench (12) to prevent excess travel of
the pusher block.
SYRINGE LOADING
The Pump 11 Elite Series can accommodate up to two
syringes. Depending on the mechanism, any type of
syringe, glass, plastic or stainless steel can be used.
The Pump 11 Elite OEM Module can accommodate
from 0.5 µl to 60 ml single syringe and 0.5 µl to 10 ml
dual syringe. The syringe barrel clamp should be flipped
for syringes larger than 30 ml.
1. Press the release button (1) and move the
pusher block (2) to the approximate length of
the syringe.
2. Unscrew the bracket clamping knobs (7,8)
to loosen the retaining brackets (4,5) on the
pusher block (2) and the Syringe holder block
(3).
3. Unscrew the syringe barrel clamp by
unscrewing the syringe barrel clamp knob (6).
4. Place the syringe on the syringe holder block
(3). Ensure the barrel flange is with in the
syringe holder’s block’s retaining bracket (5)
and the plunger flange is in the pusher block’s
retaining bracket.
*The Pico Plus Elite can accomodate from 0.5 µl
to 10 ml.
5. Screw down the syringe clamp knob (6)
!CAUTION: BE SURE MECHANICAL
STOP COLLARS ARE POSITIONED
PROPERLY TO PREVENT THE
PLUNGER FROM BOTTOMING OUT
ON GLASS SYRINGES.
> 30 cc syringes
< 30 cc syringes
2 4 5 3
6
7
1
9
10
8
11
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The syringe barrel clamp (10) may need to be
flipped depending on your syringe size. To
do so, completely unscrew and remove the
syringe barrel clamp knob from the syringe
block. Then remove lock nut (11). Flip the
bracket and reassemble.
12

11
Operating the Pump 11 Elite Series
EXTERNAL PUMP CONTROL
This section of the Pump 11 Elite Series manual
describes the control of the pump using an external
computer device. If communicating via the USB Port,
see Appendix C for one time installation instructions of
USB Driver.
Setting Up HyperTerminal
HyperTerminal is a Windows application designed to
support the external control of devices such as the
Pump 11 Elite Series pump through a USB connection.
The following instructions describe the configuration of
the HyperTerminal application.
1. Select “Start – All Programs – Accessories –
Communications – HyperTerminal”.
2. Enter a name for a New Connection
(i.e Pump_11_Elite), then click OK.
4. Set up the Port Settings as shown below and
click OK.
3. Select the Virtual Comm Port from the
“Connect using” drop-down list. Click OK.
(If the Virtual Comm Port is not known, use
Device Manager to find it. Instructions on
setting up the Virtual CommPort Driver
are supplied in Appendix E.)
5. Verify the Settings are as shown below.
6. Choose ASCII Setup and select “Echo typed
characters locally”then click OK to complete
the setup.
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To determine the proper comport, in your
PCs “Systems Properties” screen, under the
“Hardware” tab, select “Device Manger”.
Expand the “ports (COM & LPT)” section and
you should see “Elite”. The comport is listed
next to this in parenthesis.
NOTE: To determine if your pump is
connected correctly, in Hyperterminal’s
main window press ‘Enter’; a ‘:’ should
appear. If not connected properly, close
Hyperterminal, turn off the pump, unplug
then re-plug the USB cable, turn on the pump
and re-open Hyperterminal.

12
Operating the Pump 11 Elite Series
PUMP CHAIN COMMANDS
The Pump Chain commands allow all pump control
information to be managed from an external computer
source. These commands can also be used to control
a series of pumps (up to 100) from a single computer
interface.
Pump 11 Elite Series commands are communicated to
the pump via a USB port interface through a terminal
Method such as Harvard Apparatus’ Method Manager
or HyperTerminal. In using the Pump Chain commands,
you will need to assign each pump in the pump chain a
unique address, using the Set Pump Address command
in the Settings menu. The address range is from 00 to
99. This address value is used to identify which pump is
to receive a command and which pump is responding.
The first pump in the chain, the one connected to
the computer, must use address 0 (zero). Configure
each pump with its assigned address and baud rate as
described in the Pump Settings section of this guide.
Using the Pump 11 Elite Series
Pump Chain Commands
The following instructions will help you to utilize the
Pump Chain commands feature on the Pump 11 Elite
Series pump.
Commands may be abbreviated to the first four letters,
i.e. address would be abbreviated addr. A space must
follow the command if arguments are included.
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In the command list below, the following convention is
used:
{ } Required parameter
[ ] Optional parameter
| Separator between parameter
choices
# Numeric value without preceding
zeros
### Numeric value with preceding zeros
#-# A range of values
<cr> Carriage return
<lf> Line feed
<sp> Space
[prefix] Pump address prefix in the format
#: if the pump address is not zero
<prompt> Prompt (see below)
The following prompts are returned after a command is
executed:
: The pump is idle
> The pump is infusing
< The pump is withdrawing
* The pump stalled
T* The target was reached
If the pump is in poll REMOTE mode, there will be no
prompts or carraige returns.
If the pump is in poll ON mode, a XON character is
added after the prompt.
If the pump address is nonzero, the pump address is
prefixed to the prompt without a colon.
In the following list of commands:
• Thepumpaddressisprexedtoevery
response line followed by a colon.
• <lf>[##]<prompt>istheresponseunless
otherwise noted.
If the pump address is nonzero, the one or two-digit
pump address precedes the command. For example, to
set the infuse rate for pump 12, the command would
look like “12irat 3.2 u/m”.
Every line of the response from a pump with a nonzero
address is prefixed by the two digit pump address
(prefixed with a 0 if necessary) followed by a colon. No
colon is used to seperate the pump address from the
prompt however.
NOTE: When daisy-chaining using the optional
RJ-11 connectors, all pumps must use the same
baud rate. Values above 38400 are recommended.
If the USB port is used on a pump with a nonzero
address, the pump address does not need to be
used.
NOTE: If you are using the optional RJ-11
connections for daisy-chaining, the connection from
the computer must come through either the RJ-11
or RS-232 connectors.
NOTE: Once communication is established,
if you manipulate the touch screen, you must
reestablish communications to the PC
(<CR> will do this)
NOTE: System commands and start/stop
commands can be executed from Method or
Method configuration screens. However, for
parameter commands (rate, dia, vol, etc) it
is necessary to execute from the Quick Start
configuration screen.
NOTE: To maximize communication speed [as fast
as 50 ms rate changes]: (a) prefix commands with
the @ symbol to turn off GUI updates (ex: @irate
100 u/m) (b) use the ‘NVRAM off’ command to
turn off writes of rate to memory.

13
Operating the Pump 11 Elite Series
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Error Messages
Error messages are displayed if the entered command
cannot be executed for some reason. The error message
will take up two lines with the first line being the
message type and the second line describing the error
itself. The second line may be up to 80 characters long.
Command Errors
Command errors are displayed when the command is
unrecognized, entered in the wrong mode, or the state
of the pump keeps the command from executing (i.e.
using the IRUN command if a limit switch is active).
The command error has the following format:
<lf>[##:]Command error:<cr>
<lf>[##:]<sp><sp><sp>{error message}<cr>
<lf>[##]<prompt>
Argument Errors
Argument errors are displayed when a command
argument is unrecognized or out of range. The argument
in question will be displayed except in the case of
missing arguments.
The argument error has the following format:
<lf>[##:]Argument error: [bad argument]<cr>
<lf>[##:]<sp><sp><sp>{error message}<cr>
<lf>[##]<prompt>
System commands
ADDRESS
Sets or displays the pump address. Valid range is 0 to
99.
Command format:
address [0-99]
Query response:
<lf>[##:]Pump address is #<cr>
<lf>[##]<prompt>
Notes: Pumps with an address of 0 are masters, and
pumps with an address between 1 and 99 are slaves.
BAUD
Sets or displays the baud rate. Valid baud rates are
9600, 19200, 38400, 57600, 115200, 128000, 230400,
256000, 460800, and 921600. Note that some
computers may not be able to handle baud rates above
115200.
Command format:
baud [9600 | 19200 | 38400 | 57600 | 115200 |
128000 | 230400 | 256000 | 460800 | 921600]
Query response:
<lf>[##:]# baud<cr>
<lf>[##]<prompt>
BOOT
Enter the bootloader.
Command format:
boot
CATALOG
Displays a catalog of Methods stored in the pump.
Command format:
cat
Query response:
<lf>[##:]Method name Size<cr>
<lf>[##:]--------------- ----<cr>
<lf>[##:]{Method name} #<cr> This line is
repeated for each file on the disk
<lf><lf>[##:]# file(s) using # steps<cr>
<lf>[##]<prompt>
Notes: The file size is the number of steps in the Method
plus one for the file information header.
The last line shows the number of files and the total
number of steps being used on the disk.
DELMETHOD
Deletes a Method stored in the pump.
Command format:
delMethod {Method}
ECHO
Sets or displays the USB echo state. Valid states are on
or off.
Command format:
echo [on|off]
Query response:
<lf>[##:] OFF<cr>
<lf>[##]<prompt>
or:
<lf>[##:] ON<cr>
<lf>[##]<prompt>
FORCE
Sets or displays the infusion force level in percent. Valid
range is 1 to 100.
Command format:
force [1-100]
Query response:
<lf>[##:]#%<cr>
<lf>[##]<prompt>

14
Operating the Pump 11 Elite Series
Pump 11 Elite OEM Module USER’S MANUAL
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FTSWITCH
Sets or displays the footswitch setting. The footswitch
may be used in a toggle on or off mode (momentary),
a press to run mode (falling), or a release to run mode
(rising).
Command format:
ftswitch [mom | rise| fall]
Query response:
<lf>[##:]Momentary<cr>
<lf>[##]<prompt>
or:
<lf>[##:]Active high<cr>
<lf>[##]<prompt>
or:
<lf>[##:]Active low<cr>
<lf>[##]<prompt>
LOAD
Loads a method.
Command format:
load
load {method name}
load qs {i|w|iw|wi}
Query response:
<lf>[##:]Quick Start - Infuse Only (qs i)<cr>
<lf>[##]<prompt>
or:
<lf>[##:]Quick Start - Withdraw Only (qs w)<cr>
<lf>[##]<prompt>
or:
<lf>[##:]Quick Start - Infuse/Withdraw (qs iw)<cr>
<lf>[##]<prompt>
or:
<lf>[##:]Quick Start - Withdraw/Infuse (qs wi)<cr>
<lf>[##]<prompt>
or:
<lf>[##:]{method name}<cr>
<lf>[##]<prompt>
METRICS
Displays the pump metrics. The operation of this
command is covered in the 11 Elite Configuration
document.
Command format:
metrics
POLL
Sets or displays the polling mode state.
When polling mode is off, prompts are displayed when
an event happens, such as a target being reached.
When polling mode is on, prompts are not displayed
when an event happens, and a XON character is output
when the pump is ready for another command
When polling mode is in remote mode, the following
occurs
• Prompts are not displayed
• Carraige returns are not displayed
• The pump address is displayed even if 0
• Echo is forced off and the echo command
is illegal
• Command format:
poll [on | off | remote]
Query response:
<lf>[##:] OFF<cr>
<lf>[##]<prompt>
or:
<lf>[##:] ON<cr>
<lf>[##]<prompt><xon>
or:
[##:] REMOTE<lf>
TIME
Sets or displays the date and time.
Command format:
time [mm/dd/yy] [hh:mm:ss]*
Query response:
<lf>[##:]##/##/## ##:##:## #M<cr>
<lf>[##]<prompt>
* 24 hour time format

15
Operating the Pump 11 Elite Series
Pump 11 Elite OEM Module USER’S MANUAL
phone [phone] fax [phone] e-mail [email protected]
web www.harvardapparatus.com Pump 11 Elite OEM Module Series User’s Manual • Publication 5420-007 Rev B
SYRMANU
Sets or displays the syringe manufacturer. Quick Start
mode only. Valide 3-character manufacturer codes are
shown in the table below:
Typing ‘syrm’ with no arguements displays the current
syringe setting.
Typing ‘syrm ?’ displays a list of manufacturers with
their associated 3-letter code.
Typing ‘syrm {code} ?’ shows a list of syringe sizes
associated with their specified manufacturer.
Command format:
syrm [? | {3 char code} ? | {volume} ul | ml]
Query response:
<lf>[##:] {manufacturer}, {diameter} mm<cr>
<lf>[##]<prompt>
or:
<lf>[##:] Custom, {diameter} mm<cr>
<lf>[##]<prompt>
or:
<lf>[##:] {code}, {diameter} mm<cr> (this line is
repeated for each syringe manufacturer)
<lf>[##]<prompt>
or:
<lf>[##:] {volume}, {unit}<cr> (this line is repeated
for each syringe size)
<lf>[##]<prompt>
VER
Displays the short version string.
Command format:
ver
Query response:
<lf>[##:] 11 Elite #.#.#.#<cr>
<lf>[##]<prompt>
VERSION
Displays the full version string.
Command format:
version
Query response:
<lf>[##:]Firmware: v#.#.#.#<cr>
<lf>[##:]Pump address: #<cr>
<lf>[##:]Serial number: C######<cr>
<lf>[##:]Device ID: ########<cr>
<lf>[##]<prompt>
Run commands
IRUN
Runs the pump in the infuse direction. Quick Start mode
only.
Command format:
irun
RRUN
Runs the pump in the opposite direction. Quick
Start mode only.
Command format:
rrun
RUN
Simulates a key press of the run button located in the
lower right corner of the screen.
Command format:
run
STOP / STP
Stops the pump.
Command format:
stop
stp
air Air-Tite, HSW Norm-Ject
bdg Becton Dickinson, Glass (all types)
bdp Becton Dickinson, Plasti-pak
cad Cadence Science, Micro-Mate Glass
has Harvard Stainless Steel
hm1 Hamilton 700, Glass
hm2 Hamilton 1000, Glass
hm3 Hamilton 1700, Glass
hm4 Hamilton 7000, Glass
hos Hoshi
ils ILS, Glass
nip Nipro
sge SGE (Scientific Glass Engineering)
smp Sherwood-Monoject, Plastic
tej Terumo Japan, Plastic
top Top

16
Operating the Pump 11 Elite Series
Pump 11 Elite OEM Module USER’S MANUAL
phone [phone] fax [phone] e-mail [email protected]
web www.harvardapparatus.com Pump 11 Elite OEM Module Series User’s Manual • Publication 5420-007 Rev B
WRUN
Runs the pump in the withdraw direction. Quick Start
mode only.
Command format:
wrun
Rate commands
CRATE
Displays the current rate that the motor is running at.
A valid response is returned only in dynamic situations
(while the pump is running). Quick Start mode only.
Command format:
crate
Query response:
<lf>[##:]Infusing at # xl/xxx<cr>
<lf>[##]<prompt>
or:
<lf>[##:]Withdrawing at # xl/xxx<cr>
<lf>[##]<prompt>
DIAMETER
Sets or displays the syringe diameter in mm. Quick
Start mode only.
Command format:
diameter [{syringe diameter}]
Query response:
<lf>[##:]#.#### mm<cr>
<lf>[##]<prompt>
IRAMP
Sets or displays the infusion rates while ramping. Quick
Start mode only.
Command format:
iramp [{start rate} {start units} {end rate} {end units}
{ramp time in seconds}]
Query response:
<lf>[##:]Ramp not set up.<cr>
<lf>[##]<prompt>
or:
<lf>[##:]# xl/xxx to # xl/xxx in # seconds<cr>
<lf>[##]<prompt>
IRATE
Sets or displays the infusion rate. Quick Start mode
only. The rate argument may be replaced by “max”
or “min” to set the maximum or minimum rate,
respectively. “lim” may be used to display the range
limits.
Command format:
irate [max | min | lim | {rate} {rate units}]
Query response:
<lf>[##:]# xl/xxx<cr>
<lf>[##]<prompt>
or:
<lf>[##:]# xl/xxx to # xl/xxx<cr>
<lf>[##]<prompt>
WRAMP
Sets or displays the withdraw rates while ramping.
Quick Start mode only.
Command format:
wramp [{start rate} {start units} {end rate} {end
units} {ramp time in seconds}]
Query response:
<lf>[##:]Ramp not set up.<cr>
<lf>[##]<prompt>
or:
<lf>[##:]# xl/xxx to # xl/xxx in # seconds<cr>
<lf>[##]<prompt>
WRATE
Sets or displays the withdraw rate. Quick Start mode
only. The rate argument may be replaced by “max”
or “min” to set the maximum or minimum rate,
respectively. “lim” may be used to display the range
limits.
Command format:
wrate [max | min | lim | {rate} {rate units}]
Query response:
<lf>[##:]# xl/xxx<cr>
<lf>[##]<prompt>
or:
<lf>[##:]# xl/xxx to # xl/xxx<cr>
<lf>[##]<prompt>
For commands requiring rate units, use the
following format: m, u, n, p/h, m, s. ex: m/m =
milliliter/minutes ex: n/s = nanoliter/second
To clear a ramp, use CTTIME command.

17
Operating the Pump 11 Elite Series
Pump 11 Elite OEM Module USER’S MANUAL
phone [phone] fax [phone] e-mail [email protected]
web www.harvardapparatus.com Pump 11 Elite OEM Module Series User’s Manual • Publication 5420-007 Rev B
Volume commands
CIVOLUME
Clears the infused volume. Quick Start mode only.
Command format:
civolume
CTVOLUME
Clears the target volume. Quick Start mode only.
Command format:
ctvolume
CVOLUME
Clears both the infused and withdrawn volumes. Quick
Start mode only.
Command format:
cvolume
CWVOLUME
Clears the withdrawn volume. Quick Start mode only.
Command format:
cwvolume
IVOLUME
Displays the infused volume. Quick Start mode only.
Command format:
ivolume
Query response:
<lf>[##:]# xl<cr>
<lf>[##]<prompt>
SVOLUME
Sets or displays syringe volume. Quick Start mode only.
Command format:
svolume
Query response:
<lf>[##:]#.#### ul<cr>
<lf>[##]<prompt>
or:
<lf>[##:]#.#### ml<cr>
<lf>[##]<prompt>
TVOLUME
Sets or displays the target volume. Quick Start mode
only.
Command format:
tvolume [{target volume} {volume units}]
Query response:
<lf>[##:]Target volume not set<cr>
<lf>[##]<prompt>
or:
<lf>[##:] # xl<cr>
<lf>[##]<prompt>
WVOLUME
Displays the withdrawn volume. Quick Start mode
only.
Command format:
wvolume
Query response:
<lf>[##:]# xl<cr>
<lf>[##]<prompt>
Time commands
CITIME
Clears the infused time. Quick Start mode only.
Command format:
citime
CTIME
Clears both the infused and withdrawn times. Quick
Start mode only.
Command format:
ctime
CTTIME
Clears the target time. Quick Start mode only.
Command format:
cttime
CWTIME
Clears the withdrawn time. Quick Start mode only.
Command format:
cwtime
ITIME
Displays the infused time. Quick Start mode only.
Command format:
itime
Query response:
<lf>[##:]# seconds<cr>
<lf>[##]<prompt>
or:
<lf>[##:]##:##:##<cr>
<lf>[##]<prompt>

18
Operating the Pump 11 Elite Series
Pump 11 Elite OEM Module USER’S MANUAL
phone [phone] fax [phone] e-mail [email protected]
web www.harvardapparatus.com Pump 11 Elite OEM Module Series User’s Manual • Publication 5420-007 Rev B
TTIME
Sets or displays the target time. Quick Start mode only.
Command format:
ttime [{target time}]
Query response:
<lf>[##:]Target time not set<cr>
<lf>[##]<prompt>
or:
<lf>[##:]# seconds<cr>
<lf>[##]<prompt>
or:
<lf>[##:]##:##:##<cr>
<lf>[##]<prompt>
WTIME
Displays the withdrawn time. Quick Start mode only.
Command format:
wtime
Query response:
<lf>[##:]# seconds<cr>
<lf>[##]<prompt>
or:
<lf>[##:]##:##:##<cr>
<lf>[##]<prompt>
Digital I/O commands
INPUT
Reads and displays the trigger input port status.
Command format:
input
Query response:
<lf>[##:] Low.<cr>
<lf>[##]<prompt>
or:
<lf>[##:] High.<cr>
<lf>[##]<prompt>
OUTPUT
Sets the level on the output port.
Command format:
output {1} {high | low}
Internal commands
STATUS
Displays the raw status for use with a controlling
computer.
Command format:
status
Serial Command Notes
The output is in three integer fields and one flag field,
all separated by spaces and terminated by a carriage
return/linefeed pair. The first integer is the current rate
in femtoliters per second. The second integer is the
infuse time in milliseconds. The time has a granularity
of 1 millisecond. The third integer is the infused volume
in femtoliters. All three values are for the current
direction.
The flag field consists of six flags.
Flag one is the motor direction and will be “i or I” if
the pump is infusing and “w or W” if the pump is
withdrawing. If the letter is lower case, the pump motor
is idle. If upper case, the pump motor is running.
Flag two is the limit switch status. If the infuse limit
switch was hit, “i or I” is displayed. If the withdraw limit
switch was hit, “w or W” is displayed. If no limit switch
was hit or the pump does not have limit switches, “.” is
displayed.
Flag three is the stall status and will be “S” if the pump
has stalled. Otherwise it will be “.”.
Flag four is the trigger input state and will be “T” if high
and “.” if low.
Flag five is the direction port state and will be “i or I” for
infuse and “w or W” for withdraw.
Flag six is the target reached status. It will be “T” if the
target time or volume was reached and “.” if not.
The Pump 11 Elite OEM Module does not
contain limit switches, so “.” will be
displayed.

19
Appendices
Pump 11 Elite OEM Module USER’S MANUAL
phone [phone] fax [phone] e-mail [email protected]
web www.harvardapparatus.com Pump 11 Elite OEM Module Series User’s Manual • Publication 5420-007 Rev B
Appendices
APPENDIX A: SYRINGE VOLUME/DIAMETER REFERENCE TABLE
Suggested Force Level Settings for Common Syringes
Syringe Type/Material Capacity Force Setting Common Manufacturer(s)
Plastic Syringes ≤5ml 50% BD plastic, Sherwood, Airtite, Terumo
>5ml 100%
Stainless Steel All 100% Harvard Apparatus
Glass/Glass ≤20ml 30% Cadence (Popper)
>20ml 50%
The Pump 11 Elite OEM Module can
accommodate syringes ranging from 0.5 µl to
60 ml single syringe and 0.5 µl to 10 ml dual
syringe.
NOTE: The Pico Plus Elite OEM Module can
accommodate syringes ranging from 0.5 µl to
10 ml.
Glass/Plastic ≤250µl 30% Hamilton, SGE
≤5ml 50%
>5ml 100%
Harvard Apparatus
Stainless Steel
Size Diameter
2.5 ml 4.851 mm
8 9.525
20 19.130
50 28.600
Air–Tite
HSW Norm-Ject
Size Diameter
1. ml 4.69 mm
2.5 9.65
5 12.45
10 15.9
20 20.05
30 22.9
50 29.2
Becton Dickinson
‘Plasti-pak’
Size Diameter
1. ml 4.699 mm
3 8.585
5 11.989
10 14.427
20 19.05
30 21.59
50 26.594
60 26.594
Terumo
Size Diameter
1 ml vc 6.50 mm
1.ml tb 4.70 mm
2.5 9
5 13
10 15.8
20 20.15
30 23.2
60 29.2
SGE Scientic Glass
Engineering
Size Diameter
5.µl 0.343 mm
10 0.485
25 0.728
50 1.03
100 1.457
250 2.303
500 3.257
1.ml 4.606 mm
2.5 7.284
5 10.301
10 14.567
25 23
50 27.5
Hamilton
Glass - All Types
Size Diameter
0.5 µl 0.103 mm
1 0.146
2 0.206
5 0.343
10 0.485
25 0.729
50 1.03
100 1.457
250 2.304
500 3.256
1.ml 4.608 mm
1.25 5.151
2.5 7.285
5 10.3
10 14.567
25 23.033
50 32.573
Cadence Science, Inc.
Formerly Popper & Sons
MICRO-MATE Glass
Size Diameter
0.25 ml 3.47 mm
0.5 3.62
1 4.82
2 8.91
3 8.91
5 11.71
10 14.65
20 19.56
30 22.7
50 28.02
Becton Dickinson
Glass-All Types
Size Diameter
0.5 ml 4.64 mm
1 4.64
2.5 8.66
5 11.86
10 14.34
20 19.13
30 22.7
50 28.6
Sherwood–Monoject
Plastic
Size Diameter
1.ml 4.674 mm
3 8.865
6 12.600
12 15.621
20 20.142
35 23.571
60 26.568
Hoshi
Size Diameter
1. ml 6.50 mm
2 9.10
3 10.00
5 12.60
10 15.10
20 20.45
30 22.50
50 25.60
Top
Size Diameter
1. ml 6.40 mm
2.5 9.30
5 13.10
10 15.3
20 21.0
30 23.0
50 29.0
NIPRO
Size Diameter
1. ml short 6.6 mm
1. ml long 4.7
2.5 ml 9.0
5 13.0
10 15.8
20 20.1
30 23.2
50 29.1
ILS Glass
Size Diameter
250 ul 2.303 mm
500 ul 3.26 mm
1 ml 4.606 mm
2.5 ml 7.28 mm
5 ml 10.3 mm
10 ml 14.567 mm

20
Appendices
Pump 11 Elite OEM Module USER’S MANUAL
phone [phone] fax [phone] e-mail [email protected]
web www.harvardapparatus.com Pump 11 Elite OEM Module Series User’s Manual • Publication 5420-007 Rev B
Nominal Minimum/Maximum Flow Rates for Various Syringes.
(Actual Limits will vary depending on syringe manufacturer)
Syringe Size Syringe ID Minimum
Rate Units Maximum Rate Units
0.5 µl 0.103 mm 1.26 pl/min 1.326 µl/min
1 µl 0.146 mm 2.52 pl/min 2.654 µl/min
2 µl 0.206 mm 5.10 pl/min 5.304 µl/min
5 µl 0.343 mm 14.160 pl/min 14.710 µl/min
10 µl 0.485 mm 28.260 pl/min 29.400 µl/min
25 µl 0.729 mm 63.960 pl/min 66.430 µl/min
50 µl 1.030 mm 127.700 pl/min 132.600 µl/min
100 µl 1.457 mm 255.500 pl/min 265.400 µl/min
250 µl 2.304 mm 638.900 pl/min 663.500 µl/min
500 µl 3.256 mm 1.276 nl/min 1.325 ml/min
1000 µl 4.608 mm 2.556 nl/min 2.654 ml/min
1 ml 4.699 mm 2.658 nl/min 2.760 ml/min
3 ml 8.585 mm 8.871 nl/min 9.213 ml/min
5 ml 11.99 mm 17.300 nl/min 17.970 ml/min
10 ml 14.43 mm 25.050 nl/min 26.020 ml/min
20 ml 19.05 mm 43.680 nl/min 45.360 ml/min
30 ml 21.59 mm 56.110 nl/min 58.270 ml/min
50 ml 26.59 mm 85.130 nl/min 88.400 ml/min
60 ml 26.59 mm 85.130 nl/min 88.400 ml/min
APPENDIX B: PUMP 11 ELITE OEM MODULE (SINGLE & DUAL)
MIN/MAX FLOW RATES