Harvard Apparatus 683 User manual

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Publication 5375-001-REV-F
Model ‘683’
Small Animal Ventilator
User's Manual
‘683’ Small Animal Ventilator MA1 55-0000
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EU Directives WEEE and RoHS
To Our Value Customers:
We are committe to being a goo corporate citizen. As part of that commitment,
we strive to maintain an environmentally conscious manufacturing operation. The
European Union (EU) has enacte two Directives, the first on pro uct recycling
(Waste Electrical an Electronic Equipment, WEEE) an the secon limiting the use
of certain substances (Restriction on the use of Hazar ous Substances, RoHS).
Over time, these Directives will be implemente in the national laws of each EU
Member State.
Once the final national regulations have been put into place, recycling will be offere
for our pro ucts which are within the scope of the WEEE Directive. Pro ucts falling
un er the scope of the WEEE Directive available for sale after August 13, 2005 will
be i entifie with a “wheelie bin” symbol.
Two Categories of pro ucts covere by the WEEE Directive are currently exempt
from the RoHS Directive – Category 8, me ical evices (with the exception of
implante or infecte pro ucts) an Category 9, monitoring an control instruments.
Most of our pro ucts fall into either Category 8 or 9 an are currently exempt from
the RoHS Directive. We will continue to monitor the application of the RoHS
Directive to its pro ucts an will comply with any changes as they apply.
• Do Not Dispose Product with Municipal Waste
• Special Collection/Disposal Required
WEEE/RoHS Compliance Statement
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Publication 5375-001-REV-F
Table o Contents
General Information - Warranty & Repairs 2
General Safety Summary 3
Specifications 4
Description & Features 5
Voltage Selection 6
Fuse Replacement 6
Pump Operation:
Connecting to Animal ........................................7
Selecting Rate & Volume ..................................8
Setting Volume & Rate ......................................9
Using Gas Mixtures............................................9
Creating a PEEP................................................9
Cleaning & Maintenance 10
Changing Piston & Cylinder 11-12
SUBJECT PAGE NO.
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General In ormation
Serial Numbers
All inquires concerning our product should refer to the serial number of the unit. Serial
numbers are located on the rear of the chassis.
Calibrations
All electrical apparatus is calibrated at rated voltage and frequency. While the flow will stay
calibrated, the peak will vary.
Warranty
Harvard Apparatus warranties this instrument for a period of one year from date of purchase.
At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to
workmanship or material.
This warranty does not extend to damage resulting from misuse, neglect or abuse, normal
wear and tear, or accident.
This warranty extends only to the original customer purchaser.
IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR
CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitation of
incidental or consequential damages so the above limitation or exclusion may not apply to
you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FIT-
NESS FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not
allow this limitation on an implied warranty, so the above limitation may not apply to you.
f a defect arises within the two-year warranty period, promptly contact Harvard Apparatus,
84 October H ll Road, Bu ld ng 7, Holl ston, Massachusetts 01746-1371 using our toll
free number 1-800-272-2775. Goods will not be accepted for return unless an RMA (returned
materials authorization) number has been issued by our customer service department. The
customer is responsible for shipping charges. Please allow a reasonable period of time for com-
pletion of repairs, replacement and return. f the unit is replaced, the replacement unit is cov-
ered only for the remainder of the original warranty period dating from the
purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary from
state to state.
Repair Facilities and Parts
Harvard Apparatus stocks replacement and repair parts. When ordering, please describe parts
as completely as possible, preferably using our part numbers. f practical, enclose a sample or
drawing. We offer a complete reconditioning service.
CAUTION
This ventilator is not registered with the FDA and is not for clinical use on human or veteri-
nary patients. t is intended for research use only.
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General Sa ety Summary
Please read he following safe y precau ions o ensure proper use of your ven ila or.
To avoid po en ial hazards and produc damage, use his produc only as ins ruc ed in
his manual. If he equipmen is used in a manner no specified by he manufac urer,
he pro ec ion provided by he equipmen may be impaired.
To Prevent Hazard or Injury:
UUssee PPrrooppeerr LLiiee CCoorrdd
Use only he specified line cord for his produc and make sure line cord is cer ified
for coun ry of use.
GGrroouudd tthhee PPrroodduucctt
This produc is grounded hrough he grounding conduc or of he power cord. To
avoid elec ric shock, he grounding conduc or mus be connec ed o ear h ground.
Before making any connec ions o he inpu or ou pu erminals of he produc , ensure
ha he produc is properly grounded.
MMaakkee PPrrooppeerr CCooeeccttiiooss
Make sure all connec ions are made properly and securely.
OOrriieett EEqquuiippmmeett PPrrooppeerrllyy
Do no posi ion he equipmen such ha i is difficul o reach he disconnec ing
device.
OObbsseerrvvee aallll TTeerrmmiiaall RRaattiiggss
Review he opera ing manual o learn he ra ings on all connec ions.
UUssee PPrrooppeerr FFuussee
Use only specified fuses wi h produc .
AAvvooiidd EExxppoosseedd CCiirrccuuiittrryy
Do no ouch any elec ronic circui ry inside of he produc .
DDoo NNoott OOppeerraattee wwiitthh SSuussppeecctteedd FFaaiilluurreess
If damage is suspec ed on or o he produc do no opera e he produc . Con ac qual-
ified service personnel o perform inspec ion.
PPllaaccee PPrroodduucctt ii PPrrooppeerr EEvviirroommeett
Review he opera ing manual for guidelines for proper opera ing environmen s.
OObbsseerrvvee aallll WWaarriigg LLaabbeellss oo PPrroodduucctt
Read all labels on produc o ensure proper usage.
Protective Ground
Terminal
CAUTION
Refer to Manual
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Speci ications
‘683’ Small Animal Ventilator Specifications
Rat Adjustable from 30 to 150 strokes/min while the ventilator is running
Phas Ratio 1:1 fixed
Dim nsions, 25 x 32.5 x 20 cm
H x L x W (10 x 13 x 8 in)
W ight 9.5 kg (21lbs)
Pow r 100-120 VAC/200-230 VAC, 0.7A/0.35A max., 50/60 Hz
Strok Rat Continuously variable from 30 to 150 strokes/min while the ventilator
is running, with IGHT-EMITTING DIODE ( ED) DISP AY.
Volum Adjustable from 0.2 to 5.0 cc/stroke or 1.5 to 30.0 cc/stroke while
the ventilator is running
Environm ntal:
Op rating T mp ratur 0˚C to 40˚C (32˚F to 104˚F)
Op rating Humidity 20% to 80% RH, non-condensing
Installation Cat gory II
Pollution D gr 1
Port:
Part Numb r 5121-074
Siz
ID .128" (3.25mm)
OD .188" (4.78mm)
Catalog No. 55-0000
Acc ssori s
55-5282 Overhaul Kit for 5cc Small Animal Ventilator; contains O-rings,
valve springs, lubricants, etc. to overhaul Ventilator
55-5283 Overhaul Kit for 30cc Small Animal Ventilator; contains O-rings,
valve springs, lubricants, etc. to overhaul Ventilator
73-3076 Connection Kit for Anesthesia/Pressurized Gas Connection
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Description & Features
The current MODEL 683 (NEW STYLE W TH D G TAL READOUT) is an improved
volume controlled small animal/rodent respirator that is functionally identical to previous
MODELS (680, 681, and 683 OLD STYLE W THOUT D G TAL READOUT) with the
following major changes:
• New, stronger motor and motor speed control with LED
(light emitting diode) read-out of rate (stroke rate or breaths/min).
• A new design of the vertical slide valve that has no “O”-Rings or
passive check valves and requires no adjustments.
• A new orientation allows for minimal system dead space and thus more precise
tidal volume delivery to the animal
NOTE: In any correspondence, please refer to t e ventilator as “MODEL 683 wit LED RATE
READ-OUT”
The MODEL 683 is a positive volume ventilator intended to meet the respiratory requirements
of small laboratory animals weighing up to 5 kg (11 lb), covering rodents through rabbits.
This ventilator is furnished with two interchangeable piston/cylinder assemblies of 0.2 to
5.0 ml/breath (5 CC) and 1.5 to 30.0 ml/breath (30 CC). The smaller cylinder is factory
installed, and the larger one is supplied separately. The mechanical design features a linkage
that enables the piston to touch the end of the cylinder at each stroke regardless of the
volume. This assures that all the air/gas mixture taken into the ventilator is expelled
at each stroke.
A positive mechanical slide valve is actuated by a cam on the motor to synchronize inspiration
and expiration with the motion of the piston. Any NON-EXPLOSIVE gas/air mixture can
be used with the ventilator. Connection kit (73-3076) is suggested for this connection. A
three-way slide valve is attached to the mechanism plate at the end of the cylinder and actu-
ated by a cam on the motor.
These ventilators (MODELS 680, 681, 683 OLD or NEW STYLES) are NOT TO BE
USED IN AN EXPLOSIVE ATMOSPHERE OR WITH ANY EXPLOSIVE GASES
SUCH AS ETHER OR CYCLOPROPANE, ETC. THESE VENTILATORS ARE NOT
EXPLOSION-PROOF.
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Publication 5375-001-REV-F
Voltage Selection/Fuse Replacement
VVoollttaaggee SSeelleeccttiioo
1. The ‘683’ Ventilator is equipped with a 115/230V selector feature, which is incorporat-
ed into the power entry module located on the side of the unit (see figure below). The
Ventilator is shipped from the factory with the voltage selector set at 115V. To change
voltage from 115V to 230V, follow these steps:
a. Turn off main power, and disconnect power cord.
b. Using a small flathead screwdriver, pry open the fuse access door on the power
entry module (see figure below).
c. Remove fuse holder by using the small flathead screwdriver.
d. Rotate the red fuse holder 180 deg. and install back into power entry module.
e. Close small access door firmly until fully closed. n power entry module window
(see detail below) 230V should be seen.
f. Re-install power cord.
2. Turn on main power switch located on the rear panel. The display will now illuminate.
The number displayed is the current strokes per minute rate.
FFuussee RReeppllaacceemmeett
KKeeyy Informat on
1. Make sure power cord is disconnected from the main power supply before servicing the fuse.
2. Use only Type 3AG, 1/4” x 1-1/4”, 0.75 amp, 250 volts; Type T (time delay) fuses
(Harvard Apparatus part # 5153-554 or equivalent).
Turn off power and remove power cord from power module. Use a straight blade screwdriv-
er to pry open the access door. Remove the fuse holder and then remove the fuses from this
holder as shown in the figure below. Replace fuses using only 0.75A amp, 250 volts, Type T
fuses. Then replace the fuse holder.
Note: A non-operator-replaceable fuse exists inside of t e equipment. T is fuse is rated T 40mA
250V, 5 x 20mm.
H a r v a r d A p p a r a t u s M o d e l ‘ 6 8 3 ’ S m a l l A n i m a l Ve n t i l a t o r U s e r ' s M a n u a l
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Operating the Pump
Connection to t e Animal
The vertical slide valve has four ports (see figure below):
• The top port (furthest from the chassis in new orientation) is the return channel from the
animal, which leaves the Pump at the port on the side. t is at this top port that expired
air can be collected or recycled.
• The middle port is the room air/gas channel to the animal.
• The bottom port (closest to the chassis in new orientation) is the room air or gas
mixture intake.
Note: It is important t at t e tubes to and from t e animal be as s ort as possible. T e new
orientation allows t e animal to be as close as possible to t e Ventilator for minimal dead space
in t e system.
The actual connection to the animal can be either:
• Tracheal cannula with y adapter
• ntubation cannula with y adapter
A selection of trachael cannula and endotracheal tubes are listed in the Harvard Apparatus Catalog
and on our website www.harvardapparatus.com. t is important that the volume of tubing shared
by inspired and expired air (dead space) be kept to a minimum by having the “Y” or “T”
connector as close to the animal as possible.
CHASSIS
ROOM AIR
TO ANIMAL
ANIMAL
EXHAUST
OUT
IN
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Operating the Pump (Cont’d)
Selection of Rate and Volume
Refer to the Ventilator Graph (above) to select the appropriate tidal volume and respiration
rate as a function of animal body weight. As a rough guide an adult rat will weigh between
200 and 500 grams with tidal volumes between 0.6 to 1.25 cm3with respiration rates between
66 to 210 breaths/min. The average rate is 100 breaths/min. For most rodents the 5 cc
cylinder will be adequate. For larger animals it will be necessary to change the cylinder.
Ventilator Grap
Tidal Volume vs. Weight & Rate
for Laboratory Mammals in Resting State.
(Apparatus dead space must be added)
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Operating the Pump (Cont’d)
SSeettttiigg VVoolluummee
The volume delivered is read by observing the excursion of the O-ring, on the piston, which is
closest to the valve block. This piston O-ring excursion is relative to the volume scale on the
outside of the cylinder wall. Volume change is accomplished while the Ventilator is running by
turning “volume” knob in the desired direction.
SSeettttiigg RRaattee
Adjust the “Rate” control until the desired rate appears on the LED display. Avoid running the
Ventilator at rates exceeding 150 breaths/min for periods in excess of 2 hours.
CAUTION - Do not over inflate t e animal. A good rule of t umb is to observe t e
dept of respiration in t e normal calm unanest etized animal and adjust t e volume
to approximate t is respiration dept w en t e animal is attac ed to t e ventilator.
UUssee ooff GGaass MMiixxttuurree
t is possible to introduce non-explosive air/gas mixtures into the lower room air port.
However, the source of gas mixture is usually from a tank delivering gas is at a constant rate
while the Ventilator takes in air/gas intermittently. Some sort of buffer/expansion chamber
must be provided between the tank and the Ventilator. Harvard Apparatus offers a connection
kit (73-3076) to depressurize the gas prior to entering the ventilator. This Ventilator is not
designed to take air/gas inputs under pressure.
CCrreeaattiigg aa PPoossiittiivvee EEdd--EExxppiirraattoorryy PPrreessssuurree ((PPEEEEPP))
To create a PEEP pressure when using the ventilator, simply connect a length of tubing to the
“Exhaust” port and submerge the other end in a column of water. There are two locations in
which the clip and cylinder can be installed - depending on the chosen orientation. The depth of
the end of the tubing (in cm) below the water surface will give the PEEP pressure in cmH2O.
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Maintenance
CClleeaaiigg
To clean the exterior surfaces, use a lint-free cloth to remove loose dust. Use care to avoid
scratching the clear display window. For more efficient cleaning, use a soft cloth dampened
with water or an aqueous solution of 75% isopropyl alcohol.
MMaaiitteeaaccee
No special maintenance is required other than periodic lubrication of the piston O-rings and the
slide valve with the special non-toxic grease provided. To get to the slide valve for lubrication:
1. Remove the four screws holding the valve to the 1/4" aluminum black back plate.
2. Remove the screws in front that hold the cylinder and valve together.
3. Lift entire valve assembly up and out of Ventilator.
4. Pull stainless steel valve rod free of valve block.
5. Lubricate sparingly.
MMooddeell 668833 RRooddeett VVeettiillaattoorr RReeppllaacceemmeett PPaarrttss
LLuubbrriiccaattiioo
This Model 683 Ventilator is shipped with the appropriate grease to lubricate the piston and
O-rings in the cylinders and the inside of the slide valve.
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Changing Piston and Cylinder
This Ventilator is furnished with a 5 cc piston and cylinder installed. A 30 cc cylinder and
piston is also supplied for users that require larger tidal volumes.
Note: It s extremely mportant when chang ng p ston and cyl nders that they
be al gned to be concentr c and n l ne w th the p ston shaft.
The cylinder is fastened to the 4 port vertical slide valve by means of two screws located
between the “room air inlet” port and the “to animal” port on the front of the slide valve.
For ease of mounting, there is an alignment bracket located below the valve body. When re-
attaching a valve to the 683 Ventilator, the cut-out in the valve should sit square and parallel
with the corner of the alignment bracket. This should ensure that the valve and piston shaft
are in line with each other.
The vertical slide valve is fastened to the black 1/4" aluminum sub base by four screws locat-
ed on the opposite side from the printed label. After mounting a valve; if coupling does not
engage piston shaft, loosen cylinder mounting screws and adjust cylinder position until piston
shaft enters coupling with ease.
ALIGNMENT BRACKET
VALVE
MOUNTING
SCREWS
CYLINDER
MOUNTING
SCREWS
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Changing Piston and Cylinder (Cont’d)
To remove t e cylinder and piston
1. Set the Ventilator volume to maximum.
2. Run Ventilator at lowest speed and stop when the helical coupling is exposed.
3. Using a 0.050 Allen wrench (provided), disconnect the white piston from the coupling,
leaving the coupling on the piston shaft.
4. Remove the two screws located in the front of the slide valve.
5. Remove piston and cylinder.
6. Be careful to save the O-ring that is recessed in the slide valve block between the screws.
The O-ring provides the airtight seal between the plastic cylinder and the valve block.
To install t e piston and cylinder
1. Pick out the new piston and cylinder.
2. Using pliers, remove the piston and lubricate with the grease provided.
3. nsert the lubricated piston in the cylinder so that the rear of the piston is flush with the
rear of the cylinder with the 1/8" diameter coupling stud protruding.
4. Fasten the cylinder to the valve block with the two screws, making sure the O-ring is in
place. Do not tighten these two screws completely, but just enough to hold the cylinder
in place.
5. The object of alignment is to have the stud at the end of the piston be exactly in line
with its mating hole in the helical coupling. This insures that the piston moves freely
within the cylinder without undue friction.
6. The two screws at the front of the valve block control left to right alignment while the
four screws at the rear of the slide valve control up and down alignment. By adjustment
of these 6 screws the proper alignment can be made. Remember that the screws inter-
act with one another so the procedure may have to be repeated one or more times.
7. When proper alignment is achieved, the helical coupling should be connected. f align-
ment is correct, the coupling should slide onto the piston stud without bending of the
piston rod.
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O-RING
5013-051
O-RING
5013-019
VALVE BLOCK
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Notes
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Notes
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Declaration of Conformity
Appli ation of
Coun il Dire tive(s): 73/23/EEC, 89/336/EEC
Standard(s) to whi h
Conformity is De lared:
Safety: EN 61010-1, 2nd Edition (2001)
Emissions/Immunity: EN 61326:1997 w/ A1:1998
EN 61000-4-2:1995
EN 61000-4-3:1996
EN 61000-4-4:1995
EN 61000-4-5:1995
EN 61000-4-6:1996
EN 61000-4-11:1994
EN 61000-3-2:2000 w/ A.14
EN 61000-3-3:1995 w/ 1997
Manufa turer’s Name: Harvard Apparatus
Manufa turer’s Address: 84 O tober Hill Road
Holliston, Massa husetts 01746
U.S.A.
Type of Equipment: Small Animal Ventilator
Model No.: 683
I, the undersigned, hereby de lare that the equipment spe ified above onforms
to the above Dire tive(s) and Standard(s).
EMC omplian e was evaluated by Intertek/ETL Semko.
Referen e test report file number: Box 3091685-001
Pla e: United States of Ameri a
Date: February 2006
Beth Bauman
VP Engineering / Operations
(Signature)
(Full Name)
(Position)
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Artisan Technology Group is your source for quality
new and certied-used/pre-owned equipment
• FAST SHIPPING AND
DELIVERY
• TENS OF THOUSANDS OF
IN-STOCK ITEMS
• EQUIPMENT DEMOS
• HUNDREDS OF
MANUFACTURERS
SUPPORTED
• LEASING/MONTHLY
RENTALS
• ITAR CERTIFIED
SECURE ASSET SOLUTIONS
SERVICE CENTER REPAIRS
Experienced engineers and technicians on staff
at our full-service, in-house repair center
WE BUY USED EQUIPMENT
Sell your excess, underutilized, and idle used equipment
We also offer credit for buy-backs and trade-ins
www.artisantg.com/WeBuyEquipment
REMOTE INSPECTION
Remotely inspect equipment before purchasing with
our interactive website at www.instraview.com
LOOKING FOR MORE INFORMATION?
Visit us on the web at www.artisantg.com for more
information on price quotations, drivers, technical
specications, manuals, and documentation
Contact us: (888) 88-SOURCE | [e-mail] | www.artisantg.com
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