Hitachi PRIMAIDE 1440 User manual

PRIMAIDE 1440 荧光检出器

INSTRUCTION MANUAL
FOR
PRIMAIDE 1440 FL DETECTOR
使用说明书
PRIMAIDE 1440 荧光检出器
日立仪器（大连）有限公司
Hitachi Instruments (Dalian) Co., Ltd.
中国大连市甘井子区辛寨子东街 15 号
No.15 Xinzhaizi East St. Ganjingzi District Dalian of China
Copyright C Hitachi Instruments (Dalian) Co., Ltd. 2021. 5th Edition, 2021
All rights reserved. Printed in China. Part No. 8DD-9705
・Before using the instrument, read the safety instructions and
precautions carefully.
・Be sure to observe the safety instructions in the manual and the
WARNING/CAUTION labels on the instrument.
・Keep this manual in a safe place nearby so it can be referred to
whenever needed.

NOTICE
1. Information contained in this document is subject to
change without notice for improvement.
2. This manual is copyrighted by Hitachi Instruments
(Dalian) Co., Ltd. with all rights reserved.
No part of this manual may be reproduced, transmitted or
disclosed to a third party in any form or by any means
without the express written permission of Hitachi
Instruments (Dalian) Co., Ltd.
3. Hitachi Instruments (Dalian) Co., Ltd. assumes no liability
for any direct, indirect, or consequential damages arising
from use not described in this manual.
4. This document does not provide any warranty or
permission for industrial properties or any rights to grant
license lawfully and without infringement.

- 1 -
PREFACE
Thank you very much for purchasing Primaide 1440 FL Detector.
The Primaide 1440 FL Detector is specifically designed for fluorescence
detection of sample components in a liquid chromatograph system.
Note that physiologically or biologically active samples are not applicable
to the Primaide 1440 FL Detector because of possible infection with
bacteria or viruses.
This product is intended for use by persons having a basic
knowledge of chemical analysis.
Remember that improper use of analytical instruments, chemicals
or samples would result not only in wrong analytical data but also in
consequences adverse to safety. Note that it is allowed only for
persons having a basic knowledge of chemical analysis procedures
to use this instrument.
Carefully read this instruction manual before attempting operation. For
proper use of the software, please acquaint yourself with it.
The liquid chromatograph system comprises a pump unit for delivering
mobile phases, a sample introduction unit for injecting samples
(autosampler, manual injector), a column unit for separating components
of each sample, a column oven unit for maintaining the column at a
constant temperature, and a detector unit for detecting separated sample
components as electric signals. Before using the liquid chromatograph
system, read carefully the instruction manual accompanying each of
these units so that you can carry out analysis properly.

- 2 -
ABOUT THIS MANUAL
This instruction manual describes how to use and maintain the Primaide
1440 FL Detector.
This instruction manual consists of the following sections.
IMPORTANT (Warranty, Installation, Relocation,After-sale Technical
Service, etc)
SAFETY SUMMARY (collected and summarized)
Section 1OUTLINE (Basic Operation)
Section 2FUNCTIONS (Name and Function of Each Part on FL detector)
Section 3OPERATION (Basic Operation)
Section 4MAINTENANCE & TROUBLESHOOTING (Operational Check)
Section 5SPARE PARTS (Replacement Parts and Consumables)
Section 6INDEX
APPENDIX1 INSTRATTION
APPENDIX2 DESCRIPTION ON CONTACT SIGNAL COMMUNICATION
APPENDIX3 GLOSSARY
First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the
beginning of this manual for ensuring safety in operation of
accessories.

IMPORTANT - 1
IMPORTANT
Warranty on Product
Limited Warranty
The Primaide 1440 FL Detector is warranted to be free from defects in
material or workmanship under normal use within the product
specifications indicated in this manual and under conditions given below.
This warranty is void if the software is not used according to the
instruction manual.
The manufacturer makes no warranties, either express or implied, except
as provided herein, including without limitation thereof, warranties as to
marketability, merchantability, for a particular purpose or use, or against
infringement of any patent.
No oral or written information or advice given by the manufacturer, its
dealers, distributors, agents or employees shall create a warranty or in
any way increase the scope of this warranty.
Scope of Warranty
Any parts which prove to be defective in design or workmanship during
the warranty period will be repaired, adjusted or replaced without charge.
A substitute part may be used for repair, or replacement with an
equivalent product may be made instead of repair. Such system
components as a personal computer and printer to be updated frequently
for improvement may not be available in original versions at the time of
replacement.
Note that this warranty does not apply to the instrument after it is
discarded, or if modified by the user or resold without permission from the
manufacturer, consumable parts, and any failure of lifetime-expired parts.
The manufacturer assumes no liability for any damage to data or
application software due to any possible fault or failure of this instrument.
Warranty Period
One year from the date of initial installation.
(In case a separate warranty document has been issued, the warranty
period indicated in it takes precedence over the above period.)

IMPORTANT - 2
Warranty Limitations and Exclusions
Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES, EITHER EXPRESS
OR IMPLIED, EXCEPTAS PROVIDED HEREIN, INCLUDING WITHOUT
LIMITATION THEREOF, WARRANTIES AS TO MARKETABILITY,
MERCHANTABILITY, FOR A PARTICULAR PURPOSE OR USE, OR
AGAINST INFRINGEMENT OFANY PATENT. IN NO EVENT SHALL
THE MANUFACTURER BE LIABLE FORANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OFANY NATURE, OR LOSSES OR
EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE
USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE GIVEN BY THE
MANUFACTURER, ITS DEALERS, DISTRIBUTORS, AGENTS OR
EMPLOYEES SHALL CREATE A WARRANTY OR IN ANY WAY
INCREASE THE SCOPE OF THIS WARRANTY.
Note that the following cases are excluded from the scope of this
warranty, i.e., these cases are beyond the coverage of free-of-charge
repair even during the warranty period indicated above.
(a) Failure due to operation at a place not meeting the installation
requirements specified by the manufacturer.
(b) Failure due to power supply voltage/frequency other than specified
by the manufacturer or due to abnormality in power supply.
(c) Corrosion or deterioration of the tubing due to impurities contained in
reagent, gas, air or cooling water supplied by the user.
(d) Corrosion of the electric circuits or deterioration of the optical
elements due to highly corrosive atmospheric gas.
(e) Failure due to use of software, hardware or spare parts not supplied
by the manufacturer.
(f) Failure due to use not described in the manual or improper repair not
approved by the manufacturer.
(g) Failure due to maintenance or repair by other than service personnel
qualified by the manufacturer.
(h) Failure due to relocation or transport conducted not under the
supervision of the manufacturer after the initial installation of the
instrument.
(i) Failure due to disassembly, modification or relocation not approved
by the manufacturer.
(j) Failure due to acts of God, including fire, earthquake, storm, flood,
lightning, social disturbance, riot, crime, insurrection, terrorism, war
(declared or undeclared), radioactive pollution, contamination with
harmful substance, etc.

IMPORTANT - 3
(k) Failure of the hardware, or damage to the system software,
application software, data or hard disk due to computer virus
infection.
(l) After disposal of this instrument, after its resale without prior approval
from the manufacturer, consumable parts, and failure of any part that
have reached the end of its service life.
(m)Failure due to a life-limited part that has exceeded the end of its
useful lifetime.
Limitations of Liability
Hitachi Instruments (Dalian) Co., Ltd. shall not be liable in contract or in
tort (including, without limitation, negligence, strict liability or otherwise)
for claims by third parties (other then for bodily injury or property
damage), for economic losses of any kind or for any special, incidental,
indirect, consequential, punitive or exemplary damages, arising in any
way out of the performance of, or failure to perform, this instrument, even
if Hitachi Instruments (Dalian) Co., Ltd. could foresee or has been
advised of the possibility of such damages.
Disclaimer of Liability for Industrial Properties of a Third Party
Hitachi Instruments (Dalian) Co., Ltd. does not assume any liability for a
third party’s complaint regarding infringement of any patent rights or
industrial properties with respect to products manufactured through use
of the equipment supplied by Hitachi Instruments (Dalian) Co., Ltd. or its
related companies or application of said instrument.
Installation, Relocation and After-sale Technical Service
Installation and Relocation
(a) Installation at delivery shall not be carried out by the user. It shall be
carried out by our sales representative or the engineers who have
been trained and qualified for this purpose by us in order to use the
instrument safely and accurately.
(b) Before installation, the user shall make preparations for satisfying the
installation requirements in accordance with this instruction manual.
(c) If relocation becomes necessary after initial installation (delivery),
please contact the dealer from whom you purchased the instrument
or our sales representative.

IMPORTANT - 4
After-sales Service
(a) For after-sale technical service of this instrument, please notify our
local sales representative or service office.
(b) A maintenance & service contract is available for servicing the
instrument after the warranty period has ended (service available at
charge).
(c) Maintenance parts and consumables will be supplied during the
useful life of this instrument (5 years). And even though parts
and/or units (when available) will continue to be supplied after the
specified useful life has ended (for up to 10 years), they are not
intended to extend the useful life of the instrument. Also,
substitutes may be supplied in the event of discontinuance of
manufacture of certain parts or units.
(d) Some main components of the instrument other than maintenance
parts and consumables may not be available in the event of
discontinuance of manufacture of the main unit. If repair cannot be
provided in the case of instrument malfunction, we recommend that
you consider discontinuing use and replacing the instrument.
Technical Seminars and Training Courses for Users
We offer technical seminars and training courses at either our or user’s
facilities to ensure proper and safe operation of the analytical instrument
to its full performance. For further information, contact our sales
representative. (Applicants will be charged.)
Estimated Life time of the Instrument
This instrument has a useful service life of seven (5) years after the date
of initial use (installation), which is estimated under the condition that
periodic maintenance, checkup, replacement of life-limited parts, and
repair of worn parts are carried out as specified in the instruction manual.

IMPORTANT - 5
Caution on Disposal of Instrument
Although at present the instrument does not use materials that would
directly cause environmental disruption, the environmental protection
laws and regulations may be revised or amended. When planning to
dispose of the instrument, therefore, be sure to check the latest issues of
the relevant laws and regulations or consult our sales representative.
Disposal of This Instrument and Its Parts
In the present design, this instrument does not use materials that would
directly cause environmental harm. Note, however, that the
environmental protection laws and regulations may be revised or
amended. Therefore, be sure to consult with your local Hitachi High-Tech
Science sales or service representative when planning disposal of this
instrument or its parts and accessories.
When discarding or recycling the used PC, it is the user’s responsibility to
delete all data from the hard drive.
When discarding the chargeable battery mounted on the PC, refer waste
disposal treatment servicing to an authorized waste collection agent, or
send the battery for recycling after providing insulation on its positive and
negative terminals with adhesive vinyl tape or the like.
Precautions on CE Conformity Marking
In consideration of use in the European countries, this instrument bears
the CE mark indicating the conformity to the requirements mentioned
below.
Electromagnetic Compatibility Requirement
This instrument is designed to satisfy the European Norm EN61326-1
(2013) for the CE conformity marking through conformity to the EMC
Directive 2014/30/EU.
This instrument is classified as ClassAof EN61326-1. So, this instrument
must not be used in domestic establishments nor in establishments
directly connected to a low voltage power supply network which supplies
buildings used for domestic purpose.
And this instrument is also designed to comply with table 1 "Basic
immunity test requirements" in the above European Norms. If the
instrument is used near an intense electromagnetic source, however,
interfering noise may be given to the instrument to cause an adverse

IMPORTANT - 6
effect on its performance or functionality.
Safety Requirement
This instrument conforms to the LVD Directive 2014/35/EU, and has the
CE conformity marking for it. The instrument is designed to satisfy the
European Norm N61010-1 (2010). Note this instrument falls into Class-1
Pollution Level 2 of EN61010-1 (2010). Use the instrument in a proper
operating environment with its grounding connection made as specified.
Information for Users on WEEE (only for EU Countries)
This symbol is in compliance with the Waste Electrical and
Electronic Equipment directive 2012/19/EU (WEEE).
This symbol on the product indicates the requirement NOT to
dispose of the equipment as unsorted municipal waste, but use the
return and collection systems available.
RoHS Requirement
This instrument also conforms to the RoHS Directive 2011/65/EU, and
has the CE conformity marking for it. The instrument is designed to RoHS
the European Norm EN IEC63000:2018.
Information on Disposal for Users
In the European Union
If you need to discard this product or discard user serviceable parts:
Please contact your local sales representative or distributor who will
inform you of the recycle of the product. You might be charged for the
costs arising from take-back and recycling.
In other Countries outside the EU
If you wish to discard this product, please contact your local authorities
and ask for the correct method of disposal.

IMPORTANT - 7
Other Precautions
Handling of Chemicals and Samples
(a) The user is responsible for following relevant laws and regulations in
handling, storage and disposal of chemicals and samples used in
analytical operation with this instrument.
(b) Reagents, standard solutions and accuracy-control samples shall be
handled, stored and discarded as instructed by the respective
suppliers.
(c) Physiologically or biologically active samples are not applicable to
this instrument because of possible infection with bacteria or viruses.
Disturbance by Electromagnetic Wave
This instrument conforms to Class Ain the EN standards EN61326
(2013).
Avoid installing this instrument near equipment whose data will be
affected by electromagnetic noise within the permissible range of this
standard.
Also, data of this instrument may be affected by electromagnetic noise or
the instrument itself may malfunction. In the room where this instrument
is installed, the following electric devices must not be brought: Devices
which emit radio waves such as mobile phone, transceiver, wireless
telephone and similar small-power device.

SAFETY - 1
SAFETY SUMMARY
Before using the Primaide 1440 FL Detector, be sure to read the following safety
instructions carefully.
The hazard warnings which appear on the warning labels on the product
or in the manual have one of the following alert headings consisting of a
safety alert symbol and signal word WARNING or CAUTION.
: The alert symbol shown at left precedes every signal word for hazard
warnings, and appears in safety-related descriptions in the manual.
DANGER : Indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
(This warning does not apply to this product.)
WARNING : Indicates a potentially hazardous situation which, if not avoided, can
result in death or serious injury.
CAUTION : Indicates a hazardous situation which, if not avoided, will or can result in
minor or moderate injury, or serious damage to the product.
NOTICE : Used to present warnings which are not directly related to personal
injury hazards, but concern the proper use of the product.
The following signal word NOTICE or NOTE is used to indicate
precautionary instructions concerning possible property damage.
NOTICE ：
Used to indicate a potentially hazardous situation which, if not avoided,
will or can result in damage to user’s property, serious damage to the
instrument, damage to data, or environmental pollution, though personal
injury may not be incurred.
NOTE ：
Used to indicate explanatory information for ensuring proper instrument
operation and accurate measurement while preventing any possible
damage to the instrument.
•Be sure to observe the precautionary instructions in the manuals
accompanying the instrument.
•Do not perform any operation or action other than described in these
manuals.
•On occurrence of any trouble in the instrument, notify the nearest sales
service representative of Hitachi High-Tech Science.
•Keep in mind that the hazard warnings in the manuals or on the product
cannot cover every possible case, as it is impossible to predict and
evaluate all circumstances beforehand. Always be alert and use your
common sense.

SAFETY - 2
SAFETY SUMMARY
General Safety Precautions
Safety Precautions During Use
WARNING
•If an abnormal condition such as unusual noise, odor or fumes occurs during
operation of the instrument, the power supply should be turned off immediately.
Disconnect the power cord plug from the power outlet. Then, after providing proper
safety measures as required, contact the nearest service representative of Hitachi
High-Tech Science.
Using the instrument in such an abnormal condition could result in an electric shock or
fire.
Radiation from the Laser Light Source
WARNING
•The internal CD-ROM or CD-R/W drive of the PC is provided with a laser beam
source.
In normal operation of the PC containing the laser beam source, no laser beam
harmful to human health will be emitted outside. Note, however, that if a laser beam
leaking out of the PC accidentally gets into the eye, eye injury could occur. When
using the PC, carefully read the laser-related safety precautions described in the PC
manual. Be sure to observe these instructions and the following precautions:
(a) Do not open the panel of the laser device. There are no user-serviceable parts
inside.
(b) On any other laser device, do not attempt to operate or adjust.

SAFETY - 3
SAFETY SUMMARY
WARNING Precautions in the Manual
WARNING
Ignition of Flammable Chemicals
•This instrument is not explosion-proof. In unattended operation, do
not use organic solvents having an ignition point below 70 C
•Beware of ignition hazard when using flammable chemicals such as
organic solvents.
(a) Do not bring a heat or flame source near the instrument.
(b) Well-ventilate the laboratory room where the instrument is used.
(c) Always check the following conditions. If an abnormality is found, stop
operation immediately.
Leakage of solvent or waste solution.
Leakage of solvent inside the instrument.
•When using flammable chemicals, be careful about possible ignition
due to static electricity. To prevent the build-up of static electricity,
use a conductive container for waste solution employ and provide
proper grounding connection to it.
Explosion of Vapor from Flammable Chemicals
If a flammable chemical such as organic solvent leaks from the flow
path of the instrument and its vapor concentration reaches the
explosion limit, it could cause spontaneous combustion with
dangerously explosive results.
When using a flammable and readily volatile chemical, be sure to
check for leakage from the instrument flow path and ventilate the
laboratory room adequately.

SAFETY - 4
SAFETY SUMMARY
WARNING precautions in the Manual (Continued)
WARNING
Electric Shock due to Contact with Inside of Instrument
When removing the xenon lamp cover for part replacement, etc.,
there is a risk of electric shock. Be sure to turn OFF the power switch
and disconnect the power cord from the receptacle in advance.
Electric Shock due to Improper Grounding
This instrument is designed in conformity with the specifications of
Class I in Annex H of the IEC 61010-1 (International Electrotechnical
Commission Standards) –Issue 1.
To prevent an electric shock hazard, provide a proper grounding
connection.
(a) Be sure to use the grounded 3P power cable, which is supplied as a
standard accessory for the instrument.
The use of a different type of power cable may result in an electric
shock hazard. Connect the 3P power cable to a grounded 3P power
outlet.
(b) If a grounded 3P power outlet is not available, use a grounded 3P
table tap or a 3P-2P adapter. In this case, be sure to provide proper
grounding connection.
For grounding connection, use a screw having a diameter of M4 or
higher and a turn count of 3 or more in threading, and a wire having a
thickness of 1.25 mm.
Inflammation or Injury due to Toxic, Corrosive or Stimulative Solvent
When using a toxic, corrosive or stimulative solvent, be careful not to
incur a physical inflammation or injury. For details of the properties
of each solvent and how to handle it, refer to the relevant Safety Data
Sheets (SDS). Be sure to handle each solvent properly.
(a) Wear proper protective clothes (e.g., safety goggles) so that a solvent
will not come into direct with the skin.
(b) Ventilate the laboratory room adequately to prevent accidental
inhalation of harmful solvent vapor.

SAFETY - 5
SAFETY SUMMARY
WARNING precautions in the Manual (Continued)
WARNING
Injury due to Xenon Lamp Explosion
[In use]
•When the cumulative turn-on time of the xenon lamp exceeds the
guaranteed useful lifetime, the electrodes evaporate and the
scattered matter sticks to the bulb wall, so its blackening progresses
and heat dissipation is hindered. As a result, the internal bulb
temperature (pressure) will rise abnormally to cause a danger of
burst, etc.
Replace the xenon lamp with a new one before its cumulative turn-on
time exceeds the guaranteed useful lifetime.
P/N
Part Name
Useful Lifetime
650-1500
150 W xenon lamp
500 hours
890-2019
Long-life xenon lamp
1,000 hours
[At replacement]
•Before removing the xenon lamp for replacement, turn off the xenon
lamp (turn off power to the instrument) and then wait for at least one
hour until the xenon lamp becomes sufficiently cool to reduce its
internal pressure the normal safety level.
•If a strong shock or impact is applied to the xenon lamp or if the
surface of its quartz glass part is scratched, it may explode and
scatter glass pieces, resulting in personal injury. Be sure to wear
proper protective gear such as safety goggles, safety mask, thick long
sleeves, and gloves when handling the xenon lamp.
[At mounting]
•When loosening or tightening the retaining nut for the xenon lamp, be
careful not to apply excessive force to its glass bulb part. Never hold
and turn the glass bulb part for loosening/tightening.

SAFETY - 6
SAFETY SUMMARY
WARNING precautions in the Manual (Continued)
WARNING
Injury due to Xenon Lamp Explosion
[At mounting] (Continued)
•Do not touch the quartz glass part of the xenon lamp with bare hands.
If the quartz glass part of the xenon lamp is contaminated with dust or
fingerprints, wipe it using a gauze sheet or absorbent cotton cloth
slightly moistened with high-quality alcohol. If the xenon lamp is
turned on with dust or fingerprints left on the surface of the quartz
glass part, it may cause contamination burn-in to decrease the
mechanical strength of the glass part, resulting in explosion of the
xenon lamp.
•Be sure to mount the xenon lamp in the specified direction.
If the mounting direction (polarity) is wrong, the cathode will be
consumed significantly to disable turn-on of the lamp.
Mount the lamp so that the '+' (anode) mark on it will be positioned at
the support metal of the lamp holder.
If the lamp with its cathode consumed excessively is used
continuously, pressure inside the lamp bulb may become too high,
causing possible explosion. To prevent this, replace the lamp with a
new one immediately if its cathode has been consumed substantially.
•If the nut on the lamp base and wiring part is loose, the contact
resistance between them will increase due to poor contacting. This
could generate a large amount of heat to make the lamp extremely
hot, resulting in possible explosion. To prevent this, be sure to
tighten the nut securely.
[At disposal]
•The xenon lamp is filled with high-pressure gas (approx. 1 MPa at
room temperature, approx. 4 MPa under operating condition), and
this high-pressure gas still remains in the xenon lamp after it is
demounted for replacement. For disposal of the used xenon lamp,
wrap it with a thick cloth (e.g., triple-folded cotton cloth) completely
and crush its glass part with a hammer or the like. Then, discard the
xenon lamp as a dangerous waste item properly. If the xenon lamp is
discarded without being crushed, it could explode due to possible
impact at the time of disposal, scattering glass pieces to cause
personal injury.

SAFETY - 7
SAFETY SUMMARY
CAUTION Precautions in the Manual
CAUTION
Touching Hot Part Could Result in Burns
The light source lamp and its cover become hot during operation and
remain hot for a while even after power-off.
They can severely burn you if touched.
Before replacement of the lamp, turn power off and wait for about one
hour until it cools down sufficiently.
Direct Gazing at Illuminating Xenon Lamp Could Cause Eye Damage
The xenon lamp radiates intense ultraviolet light when it is on.
If you look at the illuminating xenon lamp during coarse adjustment of
its position, your eyes could be damaged. Do not look at the xenon
lamp directly when it is lit. Be sure to wear tinted safety glasses to
prevent possible eye damage.
Heavy Instrument
This instrument weight as much as 26.7 kg.
When carrying this instrument, exercise care not to incur injury by
dropping it accidentally. Be sure to hold the right and left parts of the
instrument securely when moving it.
Prevention of Instrument Tipover
Personal injury could be incurred if the instrument tips over. When
installing the instrument, provide a proper means for preventing the
instrument from tipping over accidentally.
Fatigue due to Long-Hour Operation
If you keep working with the display monitor and keyboard for long
hours, your eyes and body will be fatigued to jeopardize your health.
When working with the display monitor for a long time, take a break
for 10 to 15 minutes per hour for health of your eyes and body.

SAFETY - 8
SAFETY SUMMARY
WARNING and CAUTION Labels on Instrument
The warning labels shown below are attached on the Primaide 1440 FL
Detector.
Read the warning labels carefully , and check the instructions on them to
attain a clear understanding with reference to actual parts. Periodically
check the appearances of these warning labels to see if they are clean to
allow easy reading over a safe distance. If any one of the warning
labels becomes illegible due to deterioration, contact your local Hitachi
High-Tech Science Corporation sales representative or service office of
Hitachi High-Tech Science Corporation sales representative for
replacement with a new one.
Rear panel
(1) Electric Shock due to
Contact with Inside of Instrument