Holland Green Science Xiros Mikro User manual

User Manual
Version 1.0 1
10107002
Xiros Mikro
Freeze Dryer
User Manual

User Manual
Version 1.0 2
Table of Contents
I. Disclaimer & Warranty ............................................................................................. 3
LIMITED WARRANTY & DISCLAIMER ............................................................................... 3
LIMITATION OF LIABILITY.................................................................................................. 5
II. Safety ...................................................................................................................... 6
III. Introduction .............................................................................................................. 7
3.1 After Sales Support.................................................................................................7
3.2 Proper Use .............................................................................................................7
IV. Technical Specifications.......................................................................................... 8
IV. Inspection................................................................................................................. 9
Packing List.......................................................................................................................9
V. Precautions............................................................................................................. 10
VII. Components ........................................................................................................... 11
7.1 Main Unit ..............................................................................................................11
7.2 Other Components................................................................................................12
7.3 Assembling the Freeze Dryer................................................................................13
7.3.1 Connect the vacuum pump to the freeze dryer ....................................................13
7.3.2 Connect the water drain hose to the freeze dryer.................................................15
7.3.3 Connect the freeze dryer to the power supply......................................................16
VIII. Operation Instructions........................................................................................... 17
8.1 Manual freeze-drying mode.......................................................................................18
8.2 Program Mode...........................................................................................................21
8.3 Defrost mode.............................................................................................................26
8.4 System settings.........................................................................................................28
8.5 History data...............................................................................................................29
IX. Fault Diagnosis....................................................................................................... 30
X. Cleaning and Maintenance .................................................................................... 31
10.1 Cleaning..................................................................................................................31
10.2 Maintenance............................................................................................................31
XI. Terms Explained........................................................................................................ 32

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I. Disclaimer & Warranty
•The intellectual property rights for this manual belongs to Holland Green Science
Corporation (hereinafter referred to as “the Company”).
•Products of the company are under the patent protection of China and other
countries and regions (inclusive of obtained patents and patents currently under
application).
•The company reserves the right to change product specifications without notice.
•The contents of this manual are subject to change without notice.
•The manual’s content must not be copied, extracted, or modified in any form without
the Company’s permission.
LIMITED WARRANTY & DISCLAIMER
Products sold by the Company are intended solely for the use(s) specified by the
manufacturer and are not to be used for any other purposes including, but not limited to,
unauthorized commercial purposes. Buyer warrants and represents that Buyer will properly
test, use, qualify, and/or validate each Product for intended use, and, to the extent
authorized, manufacture and market any final articles made from the Company’s Products in
accordance with the practices of a reasonable person who is an expert in the field, and in
strict compliance with all applicable national, state and local laws and regulations. Buyer
acknowledges and understands that the purchase of Products only conveys to Buyer the
non-transferable right for only the Buyer to use the Products purchased in compliance with
the applicable manufacturer's Published User Statement, Limited User Statement, or Limited
License, if any. While this manual contains certain representations of the potential dangers
from the instrument or equipment, Buyer is solely responsible for conducting any research
necessary to learn the hazards involved for any of its intended uses of the instrument or
equipment purchased from the Company and to properly warn its customers, employees and
other personnel who may be exposed to such instrument or equipment of any risks involved
in using or handling the instrument or equipment.
The Company warrants that this product will operate or perform substantially in conformance
with the Company’s published specifications and be free from defects in material and
workmanship, when subjected to normal, proper and intended storage and usage by
properly trained personnel, for the duration of the manufacturer’s warranty period set forth in
this product documentation, published specifications or package inserts. If a manufacturer’s
warranty period is not specified in the product documentation, published specifications or
package inserts, the warranty period shall be one (1) year from the date of Bill of Sale to
Buyer for equipment, and the earlier of any stated expiration dates or ninety (90) days for all
other products (the "Warranty Period"). The Company agrees during the Warranty Period, to
repair or replace, at the Company’s option, defective Products so as to cause the same to
operate in substantial conformance with said published specifications; provided that Buyer
shall (a) promptly notify the Company in writing upon the discovery of any defect, which
notice shall include the product model and serial number (if applicable) and details of the
warranty claim; and (b) after the Company's review, the Company will provide Buyer with
service data and/or a Return Material Authorization ("RMA"), which may include biohazard
decontamination procedures and other product-specific handling instructions, then, if
applicable, Buyer may return the defective Products to the Company with all costs prepaid
by Buyer. Replacement parts may be new or refurbished, at the election of the Company. All
replaced parts shall become the property of the Company. Shipment to Buyer of repaired or
replacement Products shall be made in accordance with the Delivery provisions of the Terms

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& Conditions agreed to by the purchase of this product. Consumables are expressly
excluded from this warranty.
Notwithstanding the foregoing, Products supplied by the Company but not manufactured by
the Company are not warranted by the Company, and the Company disclaims all warranties
associated with the accuracy of any original manufacturer or third-party supplier Product
information, but the Company agrees to assign to Buyer any warranty rights in such Product
that the Company may have from the original manufacturer or third-party supplier, to the
extent such assignment is allowed by such original manufacturer or third-party supplier.
In no event shall the Company have any obligation to make repairs, replacements or
corrections required, in whole or in part, as the result of (i) normal wear and tear, (ii)
accident, disaster or event of force majeure, (iii) misuse, fault or negligence of or by Buyer,
(iv) use of the Product in a manner for which they were not designed, (v) causes external to
the Product such as, but not limited to, power failure or electrical power surges, (vi) improper
storage and handling of the Product or (vii) use of the Product in combination with equipment
or software not supplied by the Company. If the Company determines that Product for which
Buyer has requested warranty services are not covered by the warranty hereunder, Buyer
shall pay or reimburse the Company for all costs of investigating and responding to such
request at the Company's then prevailing time and materials rates. If the Company provides
repair services or replacement parts that are not covered by this warranty, Buyer shall pay
the Company therefor at the Company's then prevailing time and materials rates.
ANY INSTALLATION, MAINTENANCE, REPAIR, SERVICE, RELOCATION OR
ALTERATION TO OR OF, OR OTHER TAMPERING WITH, THE PRODUCT PERFORMED
BY ANY PERSON OR ENTITY OTHER THAN THE COMPANY WITHOUT THE
COMPANY'S PRIOR WRITTEN APPROVAL, OR ANY USE OF REPLACEMENT PARTS
NOT SUPPLIED BY THE COMPANY, SHALL IMMEDIATELY VOID AND CANCEL ALL
WARRANTIES WITH RESPECT TO THE PRODUCT.
THE OBLIGATIONS CREATED BY THIS WARRANTY STATEMENT TO REPAIR OR
REPLACE A DEFECTIVE PRODUCT SHALL BE THE SOLE REMEDY OF BUYER IN THE
EVENT OF A DEFECTIVE PRODUCT. EXCEPT AS EXPRESSLY PROVIDED IN THIS
WARRANTY STATEMENT, THE COMPANY DISCLAIMS ALL OTHER WARRANTIES,
WHETHER EXPRESS OR IMPLIED, ORAL OR WRITTEN, WITH RESPECT TO THE
PRODUCTS, INCLUDING WITHOUT LIMITATION ALL IMPLIED WARRANTIES OF
MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE OR USE. THE
COMPANY DOES NOT WARRANT THAT THE PRODUCTS ARE ERROR-FREE OR WILL
ACCOMPLISH ANY PARTICULAR RESULT.
NOTHING IN THIS MANUAL OR IN THE TERMS AND AGREEMENT AGREED TO AT THE
TIME OF PURCHASE SHALL BE CONSTRUED TO PROVIDE FOR THE TRANSFER,
ASSIGNMENT OR ALIENATION OF THE LIMITED WARRANTIES PROVIDED BY THE
COMPANY HEREIN TO ANY OTHER PARTY OTHER THAN BUYER, INCLUDING BUT
NOT LIMITED TO, SUCCESSORS, HEIRS, SUBSIDIARIES OR ANY OTHER THIRD
PERSON.
THE WARRANTIES IDENTIFIED ABOVE ARE THE SOLE AND EXCLUSIVE
WARRANTIES WITH RESPECT TO THIS PRODUCT AND ARE IN LIEU OF ALL OTHER
WARRANTIES, EXPRESS OR IMPLIED. ALL OTHER WARRANTIES ARE EXPRESSLY
DISCLAIMED, INCLUDING, WITHOUT LIMITATION, THOSE OF NON-INFRINGEMENT,
MERCHANTABILITY, OR FITNESS FOR A SPECIFIC PURPOSE OR USE (WHETHER
ARISING FROM STATUTE, OR OTHERWISE IN LAW, OR FROM A COURSE OF

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DEALING, OR USAGE OF TRADE). THIS WARRANTY IS NOT TRANSFERABLE FROM
THE ORIGINAL PURCHASER TO A SUBSEQUENT OWNER. FURTHER, THE COMPANY
IS NOT LIABLE IN CASES OF DELIBERATE, NEGLIGENT OR ACCIDENTAL MISUSE OF
THE PRODUCTS, USE WITH INAPPROPRIATE REAGENTS OR CONSUMABLES,
DAMAGE CAUSED BY DISASTER, REPAIR OR MODIFICATIONS DONE BY ANYONE
OTHER THAN THE COMPANY. THE COMPANY DOES NOT WARRANT THAT THE
PRODUCT WILL NOT INFRINGE THE INTELLECTUAL PROPERTY RIGHTS OF A THIRD
PARTY EITHER ALONE OR IN COMBINATION WITH OTHER PRODUCTS OR IN THE
OPERATION OF ANY PROCESS. THE COMPANY’S TOTAL LIABILITY FOR BREACH OF
THESE WARRANTIES SHALL BE LIMITED TO THE GROSS PURCHASE PRICE FOR
THE PRODUCT AS STATED IN THE RELEVANT INVOICE OR ORDER UNDER WHICH
THE ACTIVITY GIVING RISE TO LIABILITY ARISES. UNDER NO CIRCUMSTANCES
SHALL THE COMPANY BE LIABLE FOR INDIRECT, INCIDENTAL, CONSEQUENTIAL,
SPECIAL OR PUNITIVE DAMAGES (INCLUDING LOSS OF BUSINESS OR PROFITS)
UNDER ANY THEORY OF LIABILITY HEREUNDER.
For claims under the warranty please contact your local supplier. You may also send the
instrument directly to manufacturer, enclosing the invoice copy and by giving reasons for the
claim.
LIMITATION OF LIABILITY
NOTWITHSTANDING ANYTHING TO THE CONTRARY CONTAINED HEREIN, THE
LIABILITY OF THE COMPANY UNDER THESE TERMS AND CONDITIONS (WHETHER
BY REASON OF BREACH OF CONTRACT, TORT, INDEMNIFICATION, OR OTHERWISE,
BUT EXCLUDING LIABILITY OF THE COMPANY FOR BREACH OF WARRANTY (THE
SOLE REMEDY FOR WHICH SHALL BE AS PROVIDED UNDER THIS SECTION) SHALL
NOT EXCEED AN AMOUNT EQUAL TO THE LESSER OF (A) THE TOTAL PURCHASE
PRICE THERETOFORE PAID BY BUYER TO THE COMPANY WITH RESPECT TO THE
PRODUCT(S) GIVING RISE TO SUCH LIABILITY OR (B) ONE HUNDRED THOUSAND
DOLLARS ($100,000), WHICHEVER IS LESS.
With respect to any software products incorporated in or forming a part of the Product
hereunder Buyer intends and agrees that such software products are being licensed and not
sold, and that the words "purchase", "sell" or similar or derivative words are understood and
agreed to mean "license", and that the word "Buyer" or similar or derivative words are
understood and agreed to mean "licensee". Notwithstanding anything to the contrary
contained herein, The COMPANY or its licensor, as the case may be, retains all rights and
interest in software products provided hereunder. See the Terms & Conditions of purchase
for details regarding such license.

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II. Safety
! DANGER (may cause serious damage to property and or casualties)
1. Please carefully read this User Manual prior to operating the instrument and
observe and verify that all connections are properly secured.
2. Utility requirements: Please ensure that the electrical connections meet the local
standards and that the power supply is compatible with the Instrument nameplate.
The power source must be properly grounded.
3. The chamber and shelves may be extremely cold or hot –please check
temperature first before opening the chamber to avoid injury from frostbite or burns.
4. Only trained refrigeration engineers may service the refrigeration system.
5. When conducting maintenance and or operations inside the chamber, ensure the
door is securely held open.
6. Ensure appropriate PPE is worn at all times. Unprotected hands are not to open or
close the door.
7. It is prohibited to use flammable liquids or gas inside or around the instrument.
The instrument is not explosion rated and should not be operated in the presence of
flammable liquids or vapours.
! WARNING (may cause property damage or personal injury)
1. Do not place heavy objects on top of or stand on the instrument.
2. Prior to operating this instrument, all operators are required to fully read this manual.
Only trained and qualified operators should use the instrument.
3. It is prohibited to install any non-manufacturer authorised software onto this
instrument
4. Power ‘OFF’ the power supply for PLC and all electrical equipment before working on
the instrument.
5. Confirm the main power is ‘OFF’ prior to opening the cabinet door.
! ATTENTION (may affect operational performance or service life)
6. The overall safe operation of the instrument is the responsibility of the owner of the
instrument and their assigned operator(s), who in turn are responsible for ensuring
the user manual guidance is applied to ensure the safety and protection of personnel
and the instrument before, during and after freeze-drying operation.
7. Timely maintenance of the instrument MUST be conducted to ensure continued safe
operation and optimise the instrument’s service life.
8. Only accredited and qualified professional repair technicians can open the instrument
or conduct required repairs. Persons performing repairs on the instrument other than
those selected or approved by the Company shall operate to void any warranty
contained hereinabove for the product.

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III. Introduction
Users are advised to carefully read this manual prior to operating the freeze dryer so
that they are aware of all precautions outlined and to ensure operation is in
accordance with the instructions contained within this manual.
3.1 After Sales Support
If problems are encountered or technical support is required when installing or using
the instrument, please contact servi[email protected]m
The company may provide technical assistance and information regarding the
instrument or equipment or service without charge at its sole discretion. Buyer
assumes sole responsibility for any reliance on or use of such assistance and
information, and the company makes no warranty thereon.
Upon contact the following information is required:
•Product serial number (located on the instrument nameplate)
•Description of issue or problem
•Method and or operating steps you have undertaken towards resolution.
•Your contact details inclusive of telephone number and email address.
3.2 Proper Use
The instrument is designed for non-residential use and is to be used only in
conjunction with accessories recommended within this manual and by the
manufacturer.

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IV.Technical Specifications
Model
10107002
Product Name
Xiros Mikro
Product Weight
80 kg
Rated Voltage
110 v/Single Phase
Rated Frequency
60 Hz
Rated Power
1550 W
Cooling Temperature
≤-40 °C
Final Vacuum
≤25 Pa (empty load)
Maximum Water Capacity
4 kg
Shelf Temperature Range
-30 °C ~ 60 °C
Tray Dimensions
450 mm x 200 mm x 12 mm
Number of Trays
9
Total Tray Area
0.81 m²
Distance Between Trays
23 mm
External Dimensions (mm)
770 (D) x 748 (W) x 507 (H)

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IV. Inspection
Packing List
Unpack the equipment carefully and check for any damage which may have arisen
during transport. In the event of identified damage, please contact
[e-mail]
The package includes the following items
Item Description
Quantity
Main Unit
1
Vacuum Hose
1
Clamps
2
Sealing Ring
2
Drain Hose
2
Tray
9
Quick Setup Card
1
CAUTION:
If there is any visible damage to the
instrument, please do not connect the
instrument to a power supply.

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V. Precautions
•The power supply should be connected to the electrical outlets safely. It is important
that anyone using this equipment do so with dry hands and dry shoes to minimize the
risk of electric shock.
•A 120VAC 60Hz power supply is required for the freeze dryer operation. Any
alternative supply voltage or frequency may damage the freeze dryer reduce its
working lifespan.
•The electrical power cord is designed to be connected to the power outlet without
knots, sharp bends, or heavy materials placed on the cord.
•If the power cord is damaged, please contact the service department at
[e-mail] for repair or replacement.
•The freeze dryer should be properly grounded according to local electrical codes.
This will minimize the risk of electric shock and fire. It is important that all power
outlets are properly grounded.
•Never operate the freeze dryer if the freeze dryer chamber door is open. Doing so
may lead to equipment failure.
•No residual water or foreign matter should be present inside the chamber prior to
freeze-drying.
•Never open the freeze-drying chamber door during the freeze-drying process. Doing
so may cause personal injury or equipment failure.
•If an abnormal sound, excessive heat, smoke, etc., is detected, stop the process
immediately, disconnect the instrument from the power supply and contact the
service department. The freeze dryer operates with a minimal level of noise when
running if any significant changes happen, please stop the processing and contact
the service department.
•If a power outage occurs when operating the freeze-dryer, open the drain valve and
let the chamber pressure return to normal before opening the door to retrieve the
product.
•When the freeze-drying process has finished, first open the drain valve and then turn
off the vacuum pump.
•Be sure to keep the sealing ring and the chamber door clean. Only clean with soapy
water and avoid using solvents or other cleaning agents.
•Do not rapidly power on/off the freeze dryer. Please wait for at least 3 minutes after
powering off the freeze dryer to power it back on again.
•It is recommended that the freeze dryer be unplugged from the wall outlet when not
in use.

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VII. Components
7.1 Main Unit
touch screen
chamber door
door lock
power switch
vacuum pump
connector KF25
vent
vacuum pump
power connector
main unit power
connector
vent
drain valve

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7.2 Other Components
•Vacuum hose and KF 25 connectors
•Clamps and sealing rings
•Vacuum pump
•Tray

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•Drain hose
7.3 Assembling the Freeze Dryer
7.3.1 Connect the vacuum pump to the freeze dryer
•The connector for the vacuum pump is located at the upper right corner at the
back of the freeze dryer (Figure 1).
Figure 1
•Insert the sealing ring into the vacuum pump connector and place the clamp
around the vacuum pump connector but do not tighten the clamp (Figure 2).
Figure 2
①

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•Connect the vacuum pump hose to the connector and then tighten the clamp as
shown is Figure 3. Please note that the clamp only needs to be done up finger
tight –excessive tightening may cause damage to the fittings.
Figure 3
•Connect the inlet port to your vacuum pump as shown in Figure 4.
Figure 4
③

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7.3.2 Connect the water drain hose to the freeze dryer
•The connector for the drain hose is located to the left side of the freeze dryer
(Figure 5).
Figure 5
•Connect the drain hose to the connector and as shown in Figures 6, 7.
Figure 6 Figure 7
①
②
③

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7.3.3 Connect the freeze dryer to the power supply
Figure 8
•Locate the power connectors for the main unit and the vacuum pump at the right
side of the freeze dryer (Figure 8).
•Connect the vacuum pump power plug to the vacuum pump power connector.
•Connect one side of the main unit power cord to the power connector and
connect the other side to the electrical power outlet.
Figure 9
•Locate the main unit power switch under the touch screen.
•Turn the power switch to “ON”. A red light will appear to indicate the power
switch is “ON” as shown in Figure 9. The touch screen should also turn on.

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VIII. Operation Instructions
Before running the freeze dryer, please check and ensure that:
•There is no residual water or foreign matter inside the chamber.
•The drain valve is closed.
•The vacuum pump is connected to the freeze dryer correctly.
•The freeze dryer main unit is connected to the power supply and the vacuum
pump is connected to the freeze dryer main unit.
•The product that needs to be freeze dried has been placed on the trays.
Figure 10
The home screen is shown once the touchscreen is powered on (Figure 10). There
are five visible control buttons on the touchscreen: Manual, Program, Defrost,
Settings, and History.
Click on to return to the home page.

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8.1 Manual freeze-drying mode
To enter the “Manual Control” page, click on the “Manual” button (Figure 11).
Figure 11
Figure 12
The “Manual Control” page has the flowing functions:
•Manual Control On/Off: click “On” to enter Manual Control Mode (Figure 12).

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•Note, once Manual Control Mode has started, you can still go into the “Program
Mode” to edit the programs. However, you will not be able to start a recipe under
the “Program Mode” when Manual Control Mode is running. A warning message
will come up if “Program Mode” is started while running in Manual Mode: “Manual
Mode is still active. Please confirm switch off”(Figure 13).
Figure 13
•Set shelf temperature and chamber vacuum as desired (Figure 14).
Figure 14

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•Turn On/Off the following equipment as desired: vacuum pump, compressor,
heater upper, heater lower, vacuum valve (Figure 15).
Figure 15
•When the freeze-drying process is finished, open the drain valve first and when
the chamber is unpressurized, open the door to retrieve the product.
•Note: After the vacuum pump has been running for more than 5 minutes, if
there’s no obvious chamber pressure change detected, an error message will
appear. This applies to both the manual and program freeze-drying mode.
•Shelf temperature, chamber temperature and chamber vacuum are displayed in
real time.
•Please note: When operating in manual control mode, please ensure that product
is fully frozen before opening the vacuum valve. Opening the vacuum valve while
there is still liquid in the chamber may damage or cause premature wear to your
vacuum pump.
•Note: the freeze-dryer will automatically start the compressor to cool the chamber
and shelves when powering on the system. Disabling the compressor can only be
done in the Manual Control mode.