Horizon Technology SPE-DEX 4790 User manual

SPE-DEX 4790® Solid Phase
Extraction System
Service Manual

1
16 Northwestern Drive, Salem, NH 03079
Telephone: [phone]
Toll-Free: [phone] USA Only
Website: www.horizontechinc.com
Copyright © 2015 by Horizon Technology, Inc.
All rights reserved. Horizon Technology, Inc. reserves the right to change the information in this document
without notice. No part of this work may be processed, reproduced, or transmitted in any form or by any
means, electronic or mechanical, including photocopying and recording, or by any information storage or
retrieval system, except as may be expressly permitted in writing by Horizon Technology, Inc.
SPE-DEX is a trademark of Horizon Technology, Inc.
All other product names herein are used for identification purposes only and are recognized as properties
(including trademarks, registered trademarks, and referenced copyrighted materials) of their respective
holders.
P/N: 29-3232 REV A
(August 2015)

2
Contents
Preface .....................................................................................................................................................4
Manual Audience and Intent................................................................................................................................. 4
Technical Support.................................................................................................................................................. 4
1. Nomenclature ...................................................................................................................................6
2. Recommended System Maintenance ................................................................................................. 7
2.1. Daily Maintenance .................................................................................................................................... 7
2.2. Weekly Maintenance ................................................................................................................................ 7
3. Troubleshooting ................................................................................................................................8
4. Preventative Maint. Kits/ Miscellaneous Accessories........................................................................ 14
5. Preventative Maintenance Procedures............................................................................................. 21
5.1. Pre-Maintenance Inspection................................................................................................................... 21
5.2. Preventative Maintenance: Module ....................................................................................................... 23
5.2.1. Replacing the Check Valve ...................................................................................................................... 23
5.2.2. Replacing the Thermistors ...................................................................................................................... 23
5.2.3. Replacing the Bottle Holder O-Ring ........................................................................................................ 24
5.2.4. Replacing the Rinse Needle..................................................................................................................... 24
5.2.5. Replacing the Collection Adapter Screws................................................................................................ 25
5.2.6. Replacing the Modified Water Plug ........................................................................................................ 25
5.2.7. Replacing the Module Feet ..................................................................................................................... 26
5.2.8. Replacing the Solvent and Water Waste Lines ....................................................................................... 26
5.3. Preventative Maintenance: System ........................................................................................................ 27
5.3.1. Check Valve Tool ..................................................................................................................................... 27
5.3.2. Thermistor Height Adjustment Tool ....................................................................................................... 27
5.3.3. Hydrophilic Solution................................................................................................................................ 28
5.3.4. Water-Waste Manifold ........................................................................................................................... 28
5.3.5. Vacuum Line Kit....................................................................................................................................... 29
5.4. Post-Maintenance Inspection ................................................................................................................. 30
6. Service Kits and Parts List................................................................................................................. 32
7. Service Replacement Procedures ..................................................................................................... 35
7.1. Replacing the Bottle Holder (PN 50-2457) .............................................................................................. 35
7.2. Replacing the Water Inlet Valve (PN 48-2607)........................................................................................ 36

3
7.3. Replacing the Downtube Assembly (PN 48-2606) .................................................................................. 37
7.4. Replacing the Sample Collect Valve (50-2498)........................................................................................ 39
7.5. Replacing the Solvent- or Sample-Waste Valve (50-2535) ..................................................................... 41
7.6. Replacing a Prewet or Rinse Valve (50-2536) ......................................................................................... 43
7.7. Replacing the Water Inlet Valve Actuator (25-0666) .............................................................................. 45
7.8. Replacing the Collection Adapter (02-0602) ........................................................................................... 47
7.9. Replacing the MAC Valve (50-2237)........................................................................................................ 48
7.10. Replacing the Exhaust Fan (PN 50-0629) ................................................................................................ 51
7.11. Replacing the Spring Motor (PN 50-2233) .............................................................................................. 52
7.12. Replacing the IR Photointerruptor (PN 16-0626).................................................................................... 54
7.13. Replacing the SPE-DEX 4790 Keypad (PN 18-0588-CE) ........................................................................... 55
7.14. Replacing the Control Circuit Board (PN 50-0145-CE) ............................................................................ 57
8. Schematics ...................................................................................................................................... 58
8.1. Nitrogen and Solvent Delivery System.................................................................................................... 58
8.2. Waste and Vacuum System..................................................................................................................... 59
8.3. Plumbing Schematics .............................................................................................................................. 60
8.3.1. MAC Valve and Water Inlet Valve Actuator ............................................................................................ 60
8.3.3. Prewet and Rinse Plumbing .................................................................................................................... 61
8.3.4. Vacuum Plumbing ................................................................................................................................... 62
8.4. Electrical Systems.................................................................................................................................... 63
8.4.1. Ribbon Cable ........................................................................................................................................... 63
8.4.2. Control Circuit Board Valve Indicators .................................................................................................... 65
8.5. Operation Code ....................................................................................................................................... 66
8.5.1. Prewet Cycle............................................................................................................................................ 66
8.5.2. Sample Processing Cycle ......................................................................................................................... 67
8.5.3. Wash Cycle .............................................................................................................................................. 68
8.5.4. Air Dry Cycle ............................................................................................................................................ 69
8.5.5. Rinse Cycle............................................................................................................................................... 70
9. Available Service Documents ........................................................................................................... 71

4
Preface
Manual Audience and Intent
This manual, which is intended for all service technicians with Horizon Technology product service
training, provides the information needed to perform maintenance on the SPE-DEX 4790. The
information may contain typographical errors or technical inaccuracies and is subject to change
without notice. Modifications or enhancements may also be made to the product at any time. For the
most current information, consult Horizon Technical Support.
Product Safety
The SPE-DEX 4790 is designed with operator safety in mind. However, the product use is at the
discretion and risk of the operator or laboratory supervisor/manager. Do not attempt to disassemble
or repair the unit before reviewing this guide. It is recommended that the SPE-DEX 4790 only be
serviced by trained service technicians and with factory approved components.
Electro-Static Discharge
To reduce the risk of electrical shock, an Electro-Static discharge mat should be used when
troubleshooting or repairing the SPE-DEX 4790.
Personal Protective Equipment
Due to the nature of use for the SPE-DEX 4790, it is recommended that proper Personal Protective
Equipment (PPE) be used when servicing it. This should include safety glasses and gloves, but may
include additional PPE based on your safety requirements.
Technical Support
Visit the Horizon website for technical information in addition to that provided in this guide:
www.horizontechinc.com. If you have questions about the SPE-DEX 4790 solid phase extraction
system that are not fully addressed in this guide or our website, please contact the Horizon
Technology Technical Customer Support Center:
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Customer Support Center hours are Monday through Friday, 8:00 a.m. to 5:00 p.m. EST. The Center
provides expert technical support including troubleshooting, repair instructions, service and
installation scheduling, and replacement part information.

5
Notes:

6
1. Nomenclature
Throughout this document, the following terms will be used which require definition:
•Module: A single SPE-DEX 4790 position capable of running a single water sample and collecting an
extract.
•Controller: The control interface for the SPE-DEX 4790. This controller may be either the stand-alone
4700 Controller or a PC-connected Envision Controller. Both are capable of controlling up to eight
Modules.
•System: A collection of up to eight (8) SPE-DEX 4790 Modules connected to a single controller and
waste system.
•Preventative Maintenance (PM): A PM is preformed yearly and is designed to maintain a full System
and through the measurement of various conditions and the replacement of commonly wearable
parts.

7
2. Recommended System Maintenance
2.1. Daily Maintenance
On a daily basis when the instrument is in routine operation:
•Clean up any spills immediately using a soft, dry cloth. Follow that by wiping the area with a
cloth made damp with a mild nonabrasive general purpose laboratory cleaning solution. Do not
spray a cleaning solution directly onto the instrument.
•Flush the Water-Waste, Solvent-Waste, and Elution vacuum pathways using warm water.
•Clean accessory components like Disk Holders, Cap Adapters, and Collection Vessels using a mild
nonabrasive general purpose laboratory cleaning solution. Follow this cleaning with a rinse of
the final elution solvent for best results.
•Ensure that all gaskets are in place and in good condition.
•Verify that all Plastic-Coated Solvent Bottles are free from defects. If needed, replace them
prior to pressurizing.
2.2. Weekly Maintenance
At the end of the week when the instrument is in routine operation:
•Check the gas supply and tubing to ensure that all of the fittings are properly seated and that the
tube is not damaged in any way.
•Check the solvent tubing and ensure that all fittings are properly seated and there is no damage.
•Check the exhaust ducting, if needed, and ensure that the attachments at both ends are secure
and free of obstruction. Verify that the ducting is not crimped or crushed by other instruments
or heavy objects.
•Flush the Water-Waste, Solvent-Waste, and Elution vacuum pathways using acetone. Follow
this with warm water.

8
3. Troubleshooting
SYMPTOM
PROBABLE CAUSE
SOLUTION
Module not displayed on the
Envision Operation Page.
Data Cable not attached.
Ensure that the Data Cable is
properly seated on both the
Module’s and the Envision’s Data
Port.
Bad Data Cable.
Connect the Data Cable to a
different port on the Envision
and see if communication can be
established. Replace Cable if
necessary.
Connect the Data Cable to a
different extractor
Prewet solvent does not
dispense.
Unfamiliar method.
Confirm method steps to ensure
that the prewet is scheduled.
Confirm that the method has
been loaded onto the extractor
in use.
Low/No pressure.
Verify that nitrogen source is set
correctly.
Verify that the Solvent Bottle
Pressure is set correctly.
Verify that all nitrogen lines are
free from kinks.
Verify that the bottle cap is tight
and that both gaskets are
present and undamaged.
Low/No solvent in connected
bottle.
Verify that enough solvent is
contained within the solvent
bottle to cover the bottom of the
solvent filter.
Solvent Sip Tube not attached to
cap.
Ensure that the sip tube is
tightened into the cap.
Clogged Solvent Filter
Cut or pull Solvent Filter off and
verify that solvent is able to be
dispensed. Replace if needed.
Kinked Solvent Delivery line
Inspect all Solvent Delivery lines,
replace if needed.
Valve not actuating.
Ensure that the valve pins are
properly seated within the
Molex Connector.
Other valves not actuating;
replace the fuse on Control
Circuit Board
Fault 80: Extractor Fault: Prewet
Solvent Delivery Fault: Check
Extractor
Solvent does not reach the top
thermistor in under 1 min.
Refer to "Prewet solvent does
not dispense."

9
SYMPTOM
PROBABLE CAUSE
SOLUTION
Prewet solvent overflows.
Incorrectly set Thermistors
Use the Thermistor Height Tool
to ensure the recommended
height is set.
Pressure is set too high.
Verify the nitrogen source
pressure is set correctly.
Verify that the Solvent Bottle
Pressure is set correctly.
Bad Thermistors.
Replace the Top Thermistor.
Prewet solvent is not drained.
Insufficient vacuum.
Verify that the vacuum source is
set correctly.
Verify that the Water-Waste
Carboy Manifold is correctly
seated.
Close the Water-Waste Carboy
Drain.
Verify the Solvent Waste vacuum
is set correctly.
Solvent-Waste valve is not
opening.
Write a short method with only 1
prewet in it and run. Replace
valve if no actuation is seen.
Prewet solvent is drained
immediately.
Waste valve is stuck open.
Place DI Water into Disk Holder
attached to a module. Watch
the waste containers and
identify which one the water is
pulled into.
Check Valve is stuck open.
Use the Check Valve Tool to
cycle the Check Valve. Place DI
Water into a Disk Holder
attached to a Module. Replace
Check Valve if necessary.
Extractor continually drains after
first Prewet.
Incorrectly set Thermistor.
Use the Thermistor Height Tool
to ensure the recommended
height.
Thermistors heights reversed
Verify that the higher of the two
Thermistors is connected to J6
on the Control Circuit Board.
Bad lower Thermistor.
Replace Thermistor.
Prewet solvent drips from the
Downtube.
Prewet valve stuck open
Increase the Solvent Bottle
Pressure to 17 psi and run a
method that fires only the
affected valve. Replace the valve
if necessary.
Sample does not dispense.
Low/No pressure.
Verify that the nitrogen source is
set correctly.
Verify that the Extractor
Pressure is set correctly.
Water Inlet Valve Actuator is
Increase Extractor Pressure to