Kojair Golden Line Series User manual

Kojair Tech Oy Tel. [phone] [e-mail]
Teollisuustie 3 Fax [phone] www.kojair.com
35700 Vilppula
FINLAND
Microbiological Safety Cabinet class II EN 12469 certified by TÜV
USER MANUAL
Serial number:
Date of delivery:
Customer:

The definition of the Microbiological Safety Cabinet is as follows:
A ventilated enclosure intended to offer protection to the user and environment from the aerosols
arising from the handling of potentially hazardous and hazardous micro-organisms, with discharge to
the atmosphere being filtered.
You are owner of one of the most modern and silent Class II Microbiological Safety Cabinets
available. The Golden Line –series is microprocessor-controlled and therewith widely customizable.
All the standard-configurations of Golden Line series are type-tested and approved by TÜV
Rheinland according to current EN –standard 12469:2000 (the standard for Biosafety cabinets)
NOTICE! Only use this product for its intended purpose.

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1. FEATURES AND SAFETY........................................................................................ 4
1.1. Introduction to the cabinet.................................................................................. 4
1.2. Construction materials....................................................................................... 5
1.3. Positioning the cabinet and safety instructions .................................................. 5
2. USING THE CABINET............................................................................................... 7
2.1. Control panel...................................................................................................... 7
2.2. What to do at first start-up.................................................................................. 9
2.3. Control panel functions.................................................................................... 12
2.3.1 User settings..................................................................................................... 13
2.4. Daily maintenance and cleaning...................................................................... 14
2.5. UV-light............................................................................................................ 16
2.5.1. instruction for UV-light...................................................................................... 17
2.5.1.1. introduction...................................................................................................... 17
2.5.1.2 UV-light button.................................................................................................. 17
2.5.1.3 timer in the menu.............................................................................................. 18

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NOTE! Only Class III Microbiological Safety Cabinets can be used for working
with Group 4 pathogens. The Kojair BW3 is a class III safety cabinet which
protects the user and the environment from Group 4 pathogens.
Please contact your local Kojair representative for more information.
1. FEATURES AND SAFETY
1.1. INTRODUCTION TO THE CABINET
Microbiological safety cabinets are intended to reduce the risk to the operator when handling hazardous
or potentially hazardous micro-organisms. They do not necessarily protect the operator from all the
hazards involved. Some types of cabinet can also protect the materials being handled in them from the
environmental contamination.
Kojair Golden Line is a Class II Microbiological Safety Cabinet operating on the recirculation principle. It
protects the products handled on the work surface from contamination and safeguards the operator
against substances hazardous to health.
Picture 1. Kojair Golden Line Class II Safety Cabinet
Examples of the uses of the cabinet are cellular culture, handling pathogenic micro-organisms, isotopes,
sterile work for operations, handling medical substances in hospitals and pharmacies as well as the
production and maintenance of electronic items in which particle-free air is a necessity.

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The basic principle of the cabinet is that the replacement air moves through the grid at the front edge of
the work surface to the bottom basin and then again to the recirculation system. Simultaneously it forms
an air curtain in the work opening, which prevents air from flowing outwards.
The recirculated air inside the cabinet is purified as it flows through the HEPA-filter and it flows laminarly
through the workspace. (See picture 2).
Picture 2. Principle of the airflow
1.2. CONSTRUCTION MATERIALS
The main construction material of the cabinet is painted mild steel. The work surface and the collecting
pan located below it are made of stainless steel (AISI 304).
The front and side walls are made of 6 mm thick laminated safety glass.
1.3. POSITIONING THE CABINET AND SAFETY INSTRUCTIONS
For more information about the cabinets safe handling and moving see the service manual part
2.2
The cabinet should be positioned so that there are no external or conflicting airflow disturbances when it
is operating such as open windows or personnel passing in front of it.
The cabinet should be switched on at least 15 minutes before starting the work so there is time to clean
the workspace. Before starting the actual work it is recommended that the work is carefully prepared so
that equipment and material are moved in and out of the cabinet as little as possible.
A continuous in-and-out movement disturbs the airflow and weakens the protection given by the cabinet.
For the cabinet to give the best possible protection, you should try to minimize rapid hand and arm
movements as much as possible.
NOTE! The Cabinet may crash. Use special attention when moving and
installing the cabinet. Take into account the center of gravity when placing
transportation equipment.

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However, it is better if the cabinet can always be in the normal operating
mode. When you are not working in the cabinet, close the front glass and
device automatically switches to half speed mode. See chapter, 2.1 Control
panel.
NOTE! Before starting work, check that HEPA-filter on the roof of the
workspace is intact.
NOTE! Beware that you do not obstruct the airflow by obstructing the grid on
the worktable. The safe working area is between the grids (see picture 3.).
Picture 3. Safe working area.
While you are working, the front glass of the cabinet should be in the working position. If the front glass
is not in the correct position, the user will hear immediate warning from the alarm. If you intend to switch
off the cabinet when you have finished working, it is better to close the front window before shutting
down the cabinet.
It is strongly recommended that gas flames are used on the left side of the work space as short time as
possible. That is because the flame can disturb the airflow and it will warm up the recirculated air, which
can cause an alarm.

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2. USING THE CABINET
2.1. CONTROL PANEL
CONTOL PANEL
CONTOL BUTTON / ALARM LED FUNCTIONAL DESCRIPTION
1. Alarm indicators
Red led is used for indicating alarms. When the fan has been
switched on the general alarm -LED lights up. The general alarm -
LED lights stays on until the air speed is sufficient. If sufficient air
speed is not reached in 5 minutes, the cabinet gives an acoustic
alarm.
Working in the cabinet is not allowed while the visual and/or
acoustic alarm is activated. If alarm goes on stop working and
contact the person in charge of the laboratory.
If the power failure occurs the general alarm LED –lights up and the
acoustic alarm will sound. Working in the cabinet is not allowed
during power failure.
1. A Exhaust air alarm
If the exhaust air speed is not correct the general alarm –LED and
the exhaust air alarm –LED will light up and the acoustic alarm will
sound.
1. B Recirculated air alarm
If the recirculated air speed is not correct the general alarm –LED
and the recirculated air alarm –LED will light up and the acoustic
alarm will sound.
1. C The front window wrong position
The front glass of the cabinet should be in the working position
during the work. If the front window is not in the correct position
both the general alarm –LED and the front window alarm –LED will
light up and the acoustic alarm will sound.
2. Alarm resetting button Not in use in this cabinet model
3. Start button
Press this button to start the cabinet. (Press for few seconds to
start)
4.
Lights
You can switch on workspace lights on using this button.

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5. Socket This button activates the socket.
6. Front window up
With this button you can lift up the front window. When you lift up
the front glass from its lowest position, it stops automatic to
operating position. It is not allowed to lift the front glass any
higher excluding while cleaning or service.
7. Lcd –display
8. Mode With this button you can go to the menu.
9.
+
With this button you can browse through menus and set values
10. General alarm
Red led is used for indicating alarms. When the fan has been
switched on the general alarm -LED lights up. The general alarm -
LED lights stays on until the air speed is sufficient. If sufficient air
speed is not reached in 5 minutes, the cabinet gives an acoustic
alarm.
Working in the cabinet is not allowed while the visual and/or
acoustic alarm is activated. If alarm goes on, stop working and
contact the person in charge of the laboratory.
11. Start fan or switch fan to ½ speed
You can switch cabinet to half speed mode using this button. The
workspace lighting will shut down and the cabinet will give an
acoustic and visual alarm. The acoustic alarm signal lasts for only a
few seconds.
12. Uv –light (option)
The UV-light socket is in the workspace back wall; if you wish to use
the UV-light, push the UV-light button on the control panel.
There are two modes for UV-light, button or timer.
For more information about using UV-light, see part 2.5.
If button is selected, the UV-light will stay on until UV-button is
pressed again. If timer is selected, the cabinet asks for shutdown
time, which is selectable between 20 ... 120 min with 10 min
interval. Time can be changed with + and - buttons and time is
selected with SET button. UV-light is switched off after selected
time.
13 Magnetic valve
Used with optional gas valve. If you use liquid gas during the
procedure, first you have to push the magnetic valve button from
the control panel. This enables the gas to flow.
The gas valve works only when the cabinet is at 1/1 speed mode.
14. Front window down You can move down the front window using this button
15. Set With this button you can confirm selection.
16.
-
With this button you can browse through menus and set values

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NOTE! Before using the cabinet, perform the installation tests (listed in
chapter –Testing in service manual) and clean the cabinet carefully. See the
cleaning instructions in section 2.4 Daily maintenance and cleaning
NOTE! Despite the automatic anti-collision system, while driving stand up or
down, do not keep your feet under the stand, and make sure that there are no
obstructions on top of the cabinet and under the electrical base stand.
2.2. WHAT TO DO AT FIRST START-UP
Check if the cabinet’s power cable is in the socket outlet (230 VAC, 50 Hz) and the other lead is in the
electric plug on the top of the cabinet. Check also that other lead of the electrical base stands own
power cable is in the socket outlet (230 VAC, 50 Hz).
Adjust the correct table top height with the electrical base stand. Adjustment can be done with control
panel of the base stand. Control panel is located in the front edge of the electrical base stand (see
picture 4.)
Picture 4. Control panel of the electrical base stand.
Using the control panel:
For safety reasons control panel has a locking function.
For safety reasons electrical base stand has been equipped with an anti-collision system. If the base
stand hits an obstacle while driving stand up or down, base stand drives automatically stand 4cm in the
opposite direction.
If the panel is locked, only a bar is lightning in the centre of the display. To unlock the control press “S”
for 1 sec, When the control is unlocked, the height will be shown in the display. Now the panel is active
for 2,5 seconds. When pressing on of the keys you can do your adjustment. Hereafter the panel will be
active for 5 seconds. When there are no activations made within the mentioned time slot, the panel will
go in to locking mode again. Also if you press “S” for unlocking too long, the panel will unlock but
immediately lock again.
The panel is divided into parallel and memory drive. The two arrow buttons are used for parallel drive
and the four buttons for memory drive (see picture 5.)

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Picture 5, Control buttons of the control panel
Normal operation:
To run the base stand up and down, press the “desk up” and desk down” arrow button, keep it pressed
until the base stand reaches the desired height. The display will count the height as the base stand is
moving and will continually show the height of the desk after stopping.
Storing a memory position:
Touch the “S” button the display will flash “S” for 3 sec. While the “S” is displayed, touch the memory
button 1, 2 or 3. The display will acknowledge by showing S1, S2 or S3 for 1sec. To abort a store
sequence press the “desk up” or desk down” arrow button while the “S” is flashing, or wait the 3 seconds
until the display automatically returns to show the height of the base stand.
Drive to a stored position:
Press memory button 1, 2 or 3 and the system will start driving to the desired memory position. Keep the
button activated until the position is reached. The display will count the height as it is driving to the
memory position.
Customizing the digital display to show actual table top height:
The panel will show 68cm as the height of the base stand as the default setting. To adjust the display
touch the “S” button and the “desk up” or “desk down” button at the same time until the display shows
the actual height of the table top.
Changing between cm and inches:
Touch the “S” button for approximately 4 seconds and the unit of measurement will change on the
display. The default setting depends on the type chosen.