Thermo Scientific AI 3000 User manual

P/N 31709392, Tenth Edition, March 2012
Thermo Scientific
AI 3000 / AS 3000
AI 3000 / AS 3000 II
3000 Series Automatic Sampling System
Operating Manual
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© 2012 Thermo Fisher Scientific Inc. All rights reserved.
AI 3000 II, AS 3000 II, TRACE 1300, TRACE 1310, TRACE GC Ultra, and FOCUS GC are trademarks of
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Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
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Release history: First edition, January 2002; Second Edition, May 2003; Third Edition, September 2003
Fourth Edition, April 2004; Fifth Edition, September 2004; Sixth Edition, May 2007; Seventh Edition,
December 2007 (see the Note (*); Eighth Edition, April 2009; Ninth Edition, May 2010; Tenth Edition,
March 2012
(*) Note: The seventh edition of this manual considers the AI 3000/AS 3000 II automatic sampling system.
For Research Use Only. Not for use in diagnostic procedures.

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Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
•installation
•re-calibration
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety
standards.
Safety
This device complies with EN 61010-1:2001, EN 61010-2:2002 according to Low Voltage Directive 2006/95/EC.
Electromagnetic Compatibility
This device complies with EN 61326-1:2006 according to directive 2004/108/EC.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
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Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument. For manufacturing location, see the label
on the instrument.

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This product is in compliance with the EU 2002/95/EC (RoHs) Directive on restriction of hazardous substances in electrical
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Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:
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Este producto es in conformidad a la Directiva 2002/95/CE de la Unión Europea sobre restricciones a la utilización de
determinadas substancias peligrosas en aparatos eléctricos y electrónicos. Este producto es marcado con el siguiente símbolo en
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IMPORTANTE Gli utenti italiani devono riferirsi alla dichiarazione di Conformità alle Direttive RAEE allegata
allo strumento.
IMPORTANT
The symbol indicates that the product must not be disposed of with the normal household wastes. Correct disposal
of this product prevents any potentially negative impact on the environmental and human health that could arise
from any inappropriate handling of the product itself.
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regulations in force in your country.

Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual ix
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Rating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
The AI 3000/AS 3000 II Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Configurations of the 3000 Series Automatic Sampling System. . . . . . . . . . . . . . 5
AI 3000 Auto-injector Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
AS 3000 Auto-sampler Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Gemini Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chapter 2 Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Turret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Injection Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Vial Capture Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Turret Movements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Washing and Waste Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Rear of the Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Sample Trays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
8-Position Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
105-Position Sample Tray Assemble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Centering Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Centering Plate for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . . . . . 18
Centering Plate for TRACE GC Ultra and FOCUS GC. . . . . . . . . . . . . . . . 19
Status LED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Contents

Contents
xAI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Chapter 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Who Performs the Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
How to Lift and Carry the Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Sampling System Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Sampling System Support for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . 23
Sampling System Support for TRACE GC Ultra. . . . . . . . . . . . . . . . . . . . . . 26
Sampling System Support for FOCUS GC . . . . . . . . . . . . . . . . . . . . . . . . . . 28
References for the Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Installing the Sampling System Support on the TRACE 1300/TRACE
1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Assembling of the Sampling System Support on the Front Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Assembling of the Sampling System Support on the Back Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Assembling of the Sampling System Support for the Gemini
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Assembling of the Sampling System Support When a TSQ Quantum
is Coupled with the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Installing the Sampling System Support on the TRACE GC Ultra . . . . . . . . . . 43
Installing the Spacer Inside the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Assembling of the Sampling System Support on the GC Right Side . . . . . . . 44
Assembling of the Sampling System Support on the GC Left Side. . . . . . . . . 44
Installing the Sampling System Support on the FOCUS GC. . . . . . . . . . . . . . . 46
Assembling of the Sampling System Support on the GC right side . . . . . . . . 46
Assembling of the Sampling System Support on FOCUS GC MS
Version. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Space Saving Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Installation of the AI 3000/AS 3000 II on the GC . . . . . . . . . . . . . . . . . . . . . . 49
Installation of the Sampling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Installation of the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Electrical Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Instrument Start-up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Chapter 4 AI 3000/AS 3000 II Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
Control from PC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Washes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Info Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Status Line. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60