Thermo Scientific AI 3000 User manual

P/N 31709392, Tenth Edition, March 2012
Thermo Scientific
AI 3000 / AS 3000
AI 3000 / AS 3000 II
3000 Series Automatic Sampling System
Operating Manual
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© 2012 Thermo Fisher Scientific Inc. All rights reserved.
AI 3000 II, AS 3000 II, TRACE 1300, TRACE 1310, TRACE GC Ultra, and FOCUS GC are trademarks of
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Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
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Release history: First edition, January 2002; Second Edition, May 2003; Third Edition, September 2003
Fourth Edition, April 2004; Fifth Edition, September 2004; Sixth Edition, May 2007; Seventh Edition,
December 2007 (see the Note (*); Eighth Edition, April 2009; Ninth Edition, May 2010; Tenth Edition,
March 2012
(*) Note: The seventh edition of this manual considers the AI 3000/AS 3000 II automatic sampling system.
For Research Use Only. Not for use in diagnostic procedures.

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Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
•installation
•re-calibration
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety
standards.
Safety
This device complies with EN 61010-1:2001, EN 61010-2:2002 according to Low Voltage Directive 2006/95/EC.
Electromagnetic Compatibility
This device complies with EN 61326-1:2006 according to directive 2004/108/EC.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
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Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument. For manufacturing location, see the label
on the instrument.

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This product is in compliance with the EU 2002/95/EC (RoHs) Directive on restriction of hazardous substances in electrical
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Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:
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Conformidad RAEE/RoHs
Este producto es in conformidad a la Directiva 2002/95/CE de la Unión Europea sobre restricciones a la utilización de
determinadas substancias peligrosas en aparatos eléctricos y electrónicos. Este producto es marcado con el siguiente símbolo en
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IMPORTANTE Gli utenti italiani devono riferirsi alla dichiarazione di Conformità alle Direttive RAEE allegata
allo strumento.
IMPORTANT
The symbol indicates that the product must not be disposed of with the normal household wastes. Correct disposal
of this product prevents any potentially negative impact on the environmental and human health that could arise
from any inappropriate handling of the product itself.
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Each EU Member State has own regulations regarding the application of these directives. Please refer to the
regulations in force in your country.

Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual ix
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Rating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
The AI 3000/AS 3000 II Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Configurations of the 3000 Series Automatic Sampling System. . . . . . . . . . . . . . 5
AI 3000 Auto-injector Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
AS 3000 Auto-sampler Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Gemini Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chapter 2 Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Turret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Injection Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Vial Capture Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Turret Movements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Washing and Waste Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Rear of the Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Sample Trays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
8-Position Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
105-Position Sample Tray Assemble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Centering Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Centering Plate for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . . . . . 18
Centering Plate for TRACE GC Ultra and FOCUS GC. . . . . . . . . . . . . . . . 19
Status LED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Contents

Contents
xAI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Chapter 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Who Performs the Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
How to Lift and Carry the Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Sampling System Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Sampling System Support for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . 23
Sampling System Support for TRACE GC Ultra. . . . . . . . . . . . . . . . . . . . . . 26
Sampling System Support for FOCUS GC . . . . . . . . . . . . . . . . . . . . . . . . . . 28
References for the Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Installing the Sampling System Support on the TRACE 1300/TRACE
1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Assembling of the Sampling System Support on the Front Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Assembling of the Sampling System Support on the Back Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Assembling of the Sampling System Support for the Gemini
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Assembling of the Sampling System Support When a TSQ Quantum
is Coupled with the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Installing the Sampling System Support on the TRACE GC Ultra . . . . . . . . . . 43
Installing the Spacer Inside the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Assembling of the Sampling System Support on the GC Right Side . . . . . . . 44
Assembling of the Sampling System Support on the GC Left Side. . . . . . . . . 44
Installing the Sampling System Support on the FOCUS GC. . . . . . . . . . . . . . . 46
Assembling of the Sampling System Support on the GC right side . . . . . . . . 46
Assembling of the Sampling System Support on FOCUS GC MS
Version. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Space Saving Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Installation of the AI 3000/AS 3000 II on the GC . . . . . . . . . . . . . . . . . . . . . . 49
Installation of the Sampling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Installation of the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Electrical Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Instrument Start-up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Chapter 4 AI 3000/AS 3000 II Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
Control from PC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Washes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Info Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Status Line. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Contents
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xi
Control Through the TRACE GC Ultra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Configuring the AI 3000/AS 3000 II. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
AI 3000/AS 3000 II Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Extended Control Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
When No Vial Abort Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Control Through the FOCUS GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Configuring the AI 3000/AS 3000 II. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
AI 3000/AS 3000 II Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Sequence of Samples with the AS 3000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Guidelines for Programming with Different Injectors . . . . . . . . . . . . . . . . . . . . 70
SSL Inlet (TRACE GC Ultra/FOCUS GC) . . . . . . . . . . . . . . . . . . . . . . . . . 70
Nano-Volumes Injections in S/SL Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Packing a SSL Liner. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
S/SL Inlet with a Merlin Microseal™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
PTV Inlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
PTV Inlet with a Merlin Microseal™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
PKD and PPKD Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
Current Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Emptying of the Waste Container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Cleaning of the Sample Tray Accessory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Cleaning the Instrument Externally. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Replacing the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Cleaning the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Automated Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Manual Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Preparing a New Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Moving Away the Sampling Unit from the Injector Module . . . . . . . . . . . . . . . 80
Abbreviations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87

Contents
xii AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific

Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xiii
P
Preface
This manual contains descriptions of the features and components of the AI 3000/AS 3000 II
sampling systems. Inside, you will find all of the information necessary for routine operation
of your sampling system. This includes operating procedures, sample injection techniques,
and diagrams and descriptions of the major components.
This manual is organized as follows:
•Chapter 1, “Introduction,” provides a basic overview of the features and options of the
AI 3000/AS 3000 II System. It also describes the available instrument configurations.
•Chapter 2, “Sampling Unit,” provides a description of the components of the sampling
unit of the AI 3000/AS 3000 II System.
•Chapter 3, “Installation,” contains the instructions for the installation of the
AI 3000/AS 3000 II on the TRACE 1300/TRACE 1310, TRACE GC Ultra and
FOCUS GC gas chromatographs, the syringe and the electrical connections with the
different units of the gas chromatographic system.
•Chapter 4, “AI 3000/AS 3000 II Control,” provides the information to control the
AI 3000/AS 3000 II from the computer or the GC. The chapter contains also the
working procedures with different injectors.
•Chapter 5, “Maintenance,” provides guidelines for the maintenance of the AI 3000/AS
3000 II.
•Abbreviations contains definitions of terms used in this manual. This also includes
abbreviations, acronyms, metric prefixes, and symbols.
•Index contains an alphabetical list of key terms and topics in this guide, including cross
references and the corresponding page numbers.

Preface
About Your System
xiv AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
About Your System
Environmental Conditions
•Internaluse
• Up to 2000 meters altitude
• Temperature 18 to 30 °C
• Maximum relative humidity between 30% and 85%
• Voltage variations must not exceed the nominal voltage by ± 10%
• Transient overloads in compliance with installation categories II
• Pollution degree according to IEC 664 (3.7.3) 2
• Protection degree IP00
WARNING Thermo Fisher Scientific systems operate safely and reliably under carefully
controlled environmental conditions. If the equipment is used in a manner not specified
by the manufacturer, the protections provided by the equipment may be impaired. If you
maintain a system outside the specifications listed in this guide, failures of many types,
including personal injury or death, may occur. The repair of instrument failures caused by
operation in a manner not specified by the manufacturer is specifically excluded from the
Standard Warranty and service contract coverage.
WARNING When, for technical reasons, it is necessary to work on instrument parts
which may involve an hazard (moving parts, components under voltage, etc.) the
authorized Technical Service must be contacted. This type of situations can be identified
because access to these parts is possible only by using a tool. The removable protective
covers bear a warning symbol suggesting to refer to the documentation accompanying the
instrument. Should an operator perform a maintenance operation, he/she must have
received proper training to carry out that specific action.
WARNING Before using dangerous substances (toxic, harmful, etc.), please read the
hazard indications and information reported in the Safety Sheet supplied by the
manufacturer referring to the relevant CAS (Chemical Abstract Service) number.
The AI 3000/AS 3000 II sampling system requires the use of several chemical products,
which are present in vials and syringes, having different hazard characteristics. Before
using these substances or replacing the syringe, please read the hazard indications and
information reported in the Safety Sheet supplied by the manufacturer referring to the
relevant CAS (Chemical Abstract Service) number.

Preface
Rating
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xv
Rating
AI 3000/AS 3000 II Automatic Sampling System
• 24 Vdc through a portable external power supply
−input 90-264 Vac; 47/63 Hz — output 24 Vdc; 70 W
Contacting Us
Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is
dedicated to the quality of our customer relationships and services.
Use http://www.thermoscientific.com address for products information.address for products
information.
Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to
contact your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer Support.
Safety Alerts and Important Information
Make sure you follow the precautionary notices presented in this guide. Safety and other
special notices appear in boxes and include the following:
WARNING This is the general warning safety symbol and safety alert word to prevent
actions that could cause personal injury. It highlights hazards to humans or the
environment. Each WARNING safety alert is preceded with this safety symbol and another
appropriate safety symbol (see “Safety Alerts and Important Information” on page xv)
Then it is followed with an appropriate safety precautionary message. When you see a
safety alert on your instrument or in the publications, please carefully follow the safety
instructions before proceeding.
CAUTION This is the safety alert word to prevent actions that may cause personal injury or
instrument damage. We use it to highlight information necessary to prevent personal
injury or damage to software, loss of data, or invalid test results; or might contain
information that is critical for optimal system performance. A CAUTION safety alert is
always preceded with an appropriate safety symbol (see “Safety Alerts and Important
Information” on page xv) Then it is followed with an appropriate safety precautionary
message. When you see a safety alert on your instrument or in the publications, please
carefully follow the safety instructions before proceeding.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.

Preface
Safety Alerts and Important Information
xvi AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury and/or instrument damage. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION in intended to prevent improper actions
that may cause personal injury and/or instrument damage. The following safety symbols may
be found on your instrument and/or in this guide:
Note Emphasizes important information about a task.
Tip Helpful information that can make a task easier.
BIOHAZARD. This symbol indicates that a biohazard will, could, or may occur.
BOOTS REQUIRED. This symbol indicates that you must wear boots when
performing a task or else physical injury could or may occur.
BURN HAZARD. This symbol indicates a hot surface could, or may incur burn
injuries.
CLOTHING REQUIRED. This symbol indicates that you should wear a work
clothing when performing a task or else physical injury could or may occur.
ELECTRICAL SHOCK HAZARD. This symbol indicates that an electrical shock
hazard could, or may occur.
EXPLOSION HAZARD. This symbol indicates an explosion hazard. This
symbol indicates this risk could or may cause physical injury.
FIRE HAZARD. This symbol indicates a risk of fire or flammability, or that
fire/flammability damage will, could, or may occur.
FLAMMABLE GAS HAZARD. This symbol alerts you to gases that are
compressed, liquefied or dissolved under pressure and can ignite on contact
with an ignition source. This symbol indicates this risk could or may cause
physical injury.
GLOVES REQUIRED. This symbol indicates that you must wear gloves when
performing a task or else physical injury could or may occur.
HAND AND CHEMICAL HAZARD. This symbol indicates that chemical
damage or physical injury will, could, or may occur.
HARMFUL. This symbol indicates that the presence of harmful material will,
could, or may occur.
INSTRUMENT DAMAGE. This symbol indicates that damage to the
instrument or module may occur. This damage may not be covered under the
standard warranty.

Preface
Instrument Markings and Symbols
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xvii
Instrument Markings and Symbols
Tab le 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of
them are used on the AI 3000/AS 3000 II. See the asterisk.
LIFTING HAZARD. This symbol indicates two or more people are required to
lift the object to prevent a physical injury, which could or may occur.
MATERIAL AND EYE HAZARD. This symbol indicates that eye damage will,
could, or may occur. Eye protection must be worn.
RADIOACTIVE HAZARD. This symbol indicates that the presence of
radioactive material could, or may occur.
READ MANUAL. This symbol alerts you to carefully read your instrument’s
operational instructions before usage to ensure your safety and the
instrument’s operational ability. Failing to carefully read the instructions could
or may put you at risk for a physical injury.
TOXIC SUBSTANCES HAZARD. This symbol indicates that exposure to a
toxic substance will, could,or may cause personal injury or death.
This is the general warning symbol that the ISO 3864-2 standard refers to as
the general warning signal to prevent personal injury. It is a triangle with an
exclamation mark that precedes the WARNING safety alert word. In the
vocabulary of ANSI Z535 signage, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. We use this symbol and another appropriate safety symbol to alert to an
imminent or potential hazard that could cause personal injury.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
*Alternating Current
Both direct and alternating current
Three-phase alternating current
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
3

Preface
Instrument Markings and Symbols
xviii AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
*On (Supply)
*Off (Supply)
Equipment protected throughout by DOUBLE INSULATION or
REINFORCED INSULATION (Equivalent to Class II of IEC 536)
*Instruction manual symbol affixed to product. Indicates that the user must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
Caution, risk of electric shock
*Caution, hot surface
*Caution, biohazard
In-position of a bistable push control
Out-position of a bistable push control
*Jack socket
*Symbol in compliance to the Directive 2002/96/EC on Waste Electrical
and Electronic Equipment (WEEE) placed on the european market after
August, 13, 2005.
Table 1. Instrument Marking and Symbols (Sheet 2 of 2)
Symbol Description
+-

Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual 1
1
Introduction
This chapter provides a basic overview of the features and options of the AI 3000/AS 3000 II.
It also describes the available instrument configurations.
Contents
•The AI 3000/AS 3000 II Sampling System
•Configurations of the 3000 Series Automatic Sampling System
•Technical Specifications

1Introduction
The AI 3000/AS 3000 II Sampling System
2AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
The AI 3000/AS 3000 II Sampling System
The 3000 Series Automatic Sampling System, available as AI 3000 or AS 3000, consists of the
following parts, as respectively shown in Figure 1 and Figure 2.
Figure 1. AI 3000 Auto-injector
Turret
Base
Safety Door
Injection Unit
Centering Plate
Washing and Waste Tray
8-position Sample Tray