Hospitex LICG892 User manual

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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EOS Bravo and EOS Bravo Plus
LICG895 – LICG892 – LICG890
PRELIMINARY
Service Manual
H
H
HOSPITEX DIAGNOSTICS
Via Provinciale Lucchese 145 - 50019
Sesto Fiorentino (Florence ) Italy
Tel. +39 055 374083 Fax + 39 055 374084
e-mail :
web : http://www.hospitex.com

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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INDEX
1. GENERAL VIEW
2. SERVICE MENU
3. ASSEMBLY AND ADJUSTMENT
4. ELECTRONIC AND MENCHANICAL DESCRIPTION

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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1 - GENERAL VIEW
Figure 1: Mono Cuvette

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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Figure 2: Double Cuvette

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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Figure 3: External Devices connections ( mono and double cuvette )

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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2 - SERVICE MENU
MAIN MENU’ > F5 SERVICE
F1 : WORKING PARAMETERS
F2 : MANUAL AND AUTOMATIC WASHINGS, AUTOZERO
F3 : MECHANICAL AND FUNCTIONAL TESTS
F4 : SYSTEM PARAMETERS ( ONLY FOR SERVICE )
F5 : LABORATORY HEADING AND OPERATOR’S NAME

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F1 USER PARAMETERS
WASHING STEPS : Peristaltic pump’s steps relative to the first and the second
phase of aspiration, used to wash the cuvette with liquid and air
ASPIRATION STEPS : Peristaltic pump’s steps used to aspirate the remain of the
liquid from the cuvette
OUTER PLATE TEMP. : Parameter to modify the temperature of the plate which holds
the reaction segments
CUVETTE TEMP. : Parameter to modify the temperature of the cuvette
ENABLE REACTION SHAKE : Parameter to enable the function of shake of the plate which
holds the reaction segments
( 0 = NO, 1 = YES )
PRE-HEATING DUTY-CYCLE : Parameter relative to the power impulse relative to the
resistance that is situated around the heating reel
PRINTER MODE : Parameter relative to the printing
0 : 0 = ALL THE PRINTING ARE MADE ON THE INTERNAL
PRINTER
1 : 0 = THE PRINTINGS OF THE SESSION ARE MADE ON
THE INTERNAL PRINTER AND THE PRINTINGS OF THE
REFERTS ARE MADE ON THE EXTERNAL PRINTER
1 : 1 = ALL THE PRINTING ARE MADE ON THE EXTERNAL
PRINTER
AUTOMATIC DATE : Parameter relative to the date and time from the internal PC
0 = NO
1 = ONLY DATE
2 = DATE + HOUR

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F2 WASHINGS
F1 WASH POSITION : Used to select the number of the hole used for the washings
F2 WASHES : The instrument executes a number of XX washings in automatic
F3 TUBES WASH : The instrument executes a washing cycle of the sampling circuit
F4 CUVETTE WASH : The instrument executes a washing cycle of the aspiration circuit
F5 AUTOZERO : The instrument executes the autozero of all filters on board to the
filter wheel

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F2 WASHINGS > WASH POSITION
F1 WASH POSITION : Used to select the number of hole used for the wash ( on the example, the
First hole on the first reaction segment )

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F2 WASHINGS > F2 WASHES
F2 WASHES : Used to determinate the number of XX complete washes of the hydraulic
circuit SAMPLING/ASPIRATION ( min 1, max 99 ).
This is useful when we want to wash the hydraulic circuit very accurately but
We don’t want remain in front of the instrument.

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F2 WASHINGS > F5 AUTOZERO ( MONOCUVETTE )
F5 AUTOZERO : Used to execute an autozero of the complete filter wheel.
In case that during the autozero there are no variations respect the previous
Values the cycle will be terminated without any signal.

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F2 WASHINGS > F5 AUTOZERO ( DOUBLECUVETTE )
F5 AUTOZERO : Used to execute an autozero of the complete filter wheel.
In case that during the autozero there are no variations respect the previous
Values the cycle will be terminated without any signal.

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F2 WASHINGS > F5 AUTOZERO
F5 AUTOZERO : Example of autozero with a variation in the reading.
A variation of +/- 5% is shoved with the visualization of the following voices:
ESC : ABORT – Cancels the session
F1 : REPEAT - Repeat the session of autozero
ELSE : ACCEPT – Pressing any key except the key “ ESC “
or “ F1” we accept the new value visualized
EVERY TIME THE AUTOZERO GETS BLOCKED, PRESS F1 TO
RESTART THE PROCEDURE

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F2 WASHINGS > F5 AUTOZERO
F5 AUTOZERO : Example of autozero with a problem.
In this particular case we have the lamp which is not functioning.
This can be noted since the values of all filters are very similar if not equal
to the FILTER 0 = OFFSET

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F3 DIAGNOSTICS
F1 READING TEST : Used to execute the reading tests in mV or to execute the regulation
of the offset and the gain
F2 O.D. TEST : Used to execute readings in absorbance
F3 HARDWARE TEST : Used to execute tests of MECHANICAL/ELECTRONICAL functionality
F4 PROCEDURE TEST : Execute a complete test divided in single operation
F5 : Reserved key

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F3 DIAGNOSTICS > F1 READING TEST ( MONOCUVETTE )
F1 READING TEST : Remove the aspiration needle and put it in a holder with distillated
water.
Press F3 ASPIRATE to aspirate.
After you have aspirate water, select the filter 1 = 340 nm.
You get visualized the reading of the filter in mV and in digit.
Using the trimmer of the gain we can increase or decrease the value
of the reading.
Act on the gain trimmer to have a reading 1300 mV.
You should keep in mind, that once you act on the trimmer of the
gain to modify the reading of all the filters on board to the filters
wheel.
To execute the regulation of the offset select the Filter 0 = NULL
and act on the relative trimmer to have a reading > 1 mV.
Once the regulation of the gain and the offset is done, it’s necessary
To execute an autozero to be able to memorize the new values.
THE OPERATIVE RANGE FOR EVERY SINGLE FILTER IS 500 mV ÷ 1800 mV

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F3 DIAGNOSTICS > F1 READING TEST ( DOUBLECUVETTE )
F1 READING TEST : Remove the aspiration needle and put it in a holder with distillated
water.
Press F3 ASPIRATE to aspirate.
After you have aspirate water select the channel that you want
adjust ( example F1 CUVETTE1 ) and select the filter 1 = 340 nm.
You get visualized the reading of the filter in mV and in digit.
Using the trimmer of the gain we can increase or decrease the value
of the reading.
Act on the gain trimmer to have a reading 1300 mV.
You should keep in mind, that once you act on the trimmer of the
gain to modify the reading of all the filters on board to the filters
wheel.
To execute the regulation of the offset select the Filter 0 = NULL
and act on the relative trimmer to have a reading > 1 mV.
Once the regulation of the gain and the offset is done, it’s necessary
To execute an autozero to be able to memorize the new values.
THE OPERATIVE RANGE FOR EVERY SINGLE FILTER IS 500 mV ÷ 1800 mV

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F3 DIAGNOSTICS > F3 HARDWARE TEST
F1 MOTOR ZERO : Used to execute the home position of the motor under examination
F2 MOTOR FWD : Used to move in the clock sense the motor under examination
F3 MOTOR BWD : Used to move in the anticlock sense the motor under examination
F4 VALVE ON/OFF : Used to verify the functionality of the electrovalve situated on the
diluter
Used to verify the functionality of the two pumps in the double
cuvette
F5 DISPLAY INP. : The command F5 active the continues visualisation of the inputs

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F3 DIAGNOSTICS > F3 HARDWARE TEST
LEGENDA
MOT : Number relative to the motor in object
DESCRIPTION : Description of the motor in object
ENAB : Command to block the motor in object
( 0 = FREE / 1 = BLOCKED )
SPEED : Speed of the motor in object
IX. STEPS : X Steps to execute with the motor in object
LOG. POS. : Position were we want put the motor in object
ABS. POS : Real position , in steps, that the motor has taken

Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual
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MAIN MENU’ > F5 SERVICE > F3 DIAGNOSTICS > F3 HARDWARE TEST
LOGICAL POSITION
INNER PLATE : The logical position from 0 to 31 carries the relative cups under the relative
point of sampling.
The logical position from 32 to 63 carries the relative cups under the relative
point of sampling.
OUTER PLATE : The logical position from 1 to 96 carries the relative holes of the reaction
segments under the aspiration needle.
The logical position from 101 to 196 carries the relative holes of the reaction
Segments under the point positioning of sampling arm.
The logical position from 1 to 189 carries the relative holes of the reaction
segments under the aspiration needle *
The logical position from 201 to 389 carries the external ring of the reaction
segments under the point positioning of sampling arm *
The logical position from 202 to 390 carries the internal ring of the reaction
segments under the point positioning of sampling arm *
FILTER SELECT : The logical position corresponds to the number of the relative filter
PERISTALTIC PUMP : NOT IN USE
DILUTER SYRINGE : The logical position indicates 1 uL to dispense
REAGENT PLATE : The logical position from 1 to 18 or from 1 to 27 carries the sampling arm
up to the relative bottles.
The logical position 41 sampling arm up to the inner plate internal ring.
The logical position 42 sampling arm up to the inner plate external ring.
The logical position 43 sampling arm up to the outer plate.
The logical position 43 sampling arm up to the outer plate internal ring.*
The logical position 44 sampling arm up to the outer plate external ring.*
The logical position 44 sampling arm up to the repose condition
SAMPLING NEEDLE : the logical position 3 sampling needle down in the bottom of sample cups.
The logical position 4 sampling needle down in the hole of the reaction
segments.
The logical position 8 sampling needle down in the bottom of the reagents
bottle.
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