Ibi QS-710 User manual

IBI SCIENTIFIC
QS-710 - QUICK SCREEN
HORIZONTAL GEL ELECTROPHORESIS UNIT
GEL SIZE:7CM X 10CM
IBI Catalog Number: IB51000
OPERATOR’SMANUAL
SCIENTIFIC

2
TABLE OF CONTENTS
A. SAFETY INFORMATION 3
B. PACKAGE CONTENTS 3
C. PRODUCT SPECIFICATIONS 3
D. OPERATING INSTRUCTIONS 4
PREPARATION OF THE AGAROSE GEL - DNA 4
PREPARATION OF THE AGAROSE GEL - RNA 5
CASTING THE GEL 6
REMOVING THE COMB 7
LOADING THE SAMPLES INTO THE GEL 7
ELECTRICAL CONNECTIONS TO THE SAFETY LID 8
SAMPLE ELECTROPHORESIS 8
DETECTION AND DOCUMENTATION OF SEPARATED FRAGMENTS 9
CHOICE OF BUFFER 9
VOLTAGE 10
STAINING SOLUTION 10
E. MAINTENANCE OF UNIT 11
F. REPLACEMENT PARTS & ACCESSORIES 11
QS-710 ACCESSORY ITEMS AND REPLACEMENT PARTS 11
QS-710 COMBS 12
G. RELATED IBI PRODUCTS 12
H. RELATED IBI CERTIFIED REAGENTS 12-14
I. REFERENCES 15
J. LIMITED WARRANTY 15

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A. SAFETY INFORMATION
Important Safety Information!
Please read this manual carefully before operating your new IBI QS-710 unit.
This manual contains important operating and safety information.
To best use the product, please read the entire manual carefully prior to use.
To avoid possible injury, this product should only be used for its intended purpose.
B. PACKAGE CONTENTS
Upon receiving this product, please verify all of the noted parts and accessories are contained in
this package.
Model QS-710 Buffer Tank
Patented QS-710 Vented Lid
UV Transmittant Casting Tray
Casting Fixture
One Set of 1.5mm x 8 Tooth Combs (2 ea.)
One Set of Power Cords (Red and Black)
Bubble Level
Operation Manual
NOTE: Carefully inspect all items in the package to insure no items are broken or missing. If
there are items broken, please inspect the package carefully for signs of shipping damage.
If there is ANY sign of shipping damage, please contact the carrier and file a claim with
them immediately. Contact the distributor from which you purchased the item.
C. PRODUCT SPECIFICATIONS
Height Width Length
Unit Dimensions 6.4cm 11.4cm 22.2cm
Gel Dimensions 7.0cm 10.0cm
Maximum Sample Capacity: 30 Samples - 2 Combs, 15 samples each
Buffer Capacity: 250ml
Distance Between Electrodes: 19.3cm

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D. OPERATING INSTRUCTIONS
Your new QS-710 Quick Screen Horizontal Unit is cleaned and wiped prior to packaging; however,
components should be washed in warm soapy water prior to use in the laboratory. A mild dish wash-
ing liquid, like Joy, works well.
Gently wash the tank, lid, UVT casting tray, and casting fixture in warm soapy water, taking care not
to scratch any of the acrylic components such as the tank and UVT tray. Do NOT wash Power Cords.
NOTE: It is also recommended that the UVT casting tray be cleaned with alcohol prior to use. Be
certain the entire unit is dry prior to use.
PREPARATION OF THE AGAROSE GEL - DNA
1.) Select the percentage gel necessary to effectively resolve your sample, use Table 1 as a guide.
Table 1 Gel Concentrations and Resolving Ranges
Concentration of Efficient Range of
Agarose in Gel Separation of Linear DNA
(% w/V) (Kb)
0.3% 5 - 60
0.6% 1 - 20
0.7% 0.8 - 10
0.9% 0.5 - 7
1.2% 0.4 - 6
1.5% 0.2 - 3
2.0% 0.1 - 2
* Table taken from Sambrook, J., Fritsch, E.F., & Maniatis, T. (1989)
Molecular Cloning, A Laboratory Manual, 1, 6. 8, 613.
2.) Weigh an appropriate quantity of agarose (0.3% means 0.3gm of agarose per 100ml of gel
volume) and place it into a 250ml flask.
3.) Make up 500ml of either 1X TAE or 1X TBE electrophoresis buffer. See below:
Electrophoresis Buffers
The two most commonly used buffers for horizontal electrophoresis of double stranded DNA in
agarose gels are Tris-Acetate-EDTA (TAE) [IB70160] and Tris-Borate-EDTA (TBE) [IB70150].
While the resolving powers of these buffers are very similar, the relative buffer capacities are very
different, conferring different run attributes which are summarized below:
TAE (IB70160): Tris-acetate has traditionally been the more commonly used buffer. However, its
relatively low buffer capacity will become exhausted during extended elec-
trophoresis, making buffer recirculation necessary in runs exceeding 140 mA-
hours. Potential advantages of using TAE buffer over TBE buffer include superi-
or resolution of supercoiled DNA and approximately 10 % faster migration of
double-stranded linear DNA fragments.
TBE (IB70150): Tris-borate's significantly greater buffering capacity and its relatively low current
draw eliminates the need for recirculation in all but the most extended runs (>
300 mA-hours). TBE buffer systems are not recommended when fragments are to
be recovered from the gel after electrophoresis.

5
4.) Add ethidium bromide (IB40075) to the diluted electrophoresis buffer to a final concentration
of 0.5μg/ml.
NOTE: The addition of ethidium bromide to both the gel and the running buffer will result in
maximum detection levels by providing high levels of sample fluorescence with an evenly
low level of background.
5.) Add 6.6ml of the 1X electrophoresis buffer containing ethidium bromide made in step 4 per
millimeter of gel thickness desired, up to a maximum of 50ml, to the flask containing the
agarose (IB70035-40-42-45). A 50ml gel solution will make a 7.6mm thick gel. Thinner gels
may be made, however care must be taken that the wells are deep enough to accommodate the
desired sample volume.
Sample Volume
Catalog # Comb Description Well Width Per mm Gel
IB51040 1.0mm, 8 tooth 4.7mm 4.7ul
IB51045 1.0mm, 12 tooth 2.8mm 2.7ul
IB51050 1.0mm, 15 tooth 2.2mm 2.3ul
IB51055 1.5mm, 8 tooth 4.7mm 7.0ul
IB51060 1.5mm, 12 tooth 2.8mm 4.0ul
IB51065 1.5mm, 15 tooth 2.2mm 3.3ul
IB51070 1.0mm, 0 Marker, 1 Sample 62.6mm 62.6ul
IB51075 1.0mm, 1 Marker, 1 Sample 4.7mm, 54.7mm 4.7ul, 54.7ul
IB51080 1.5mm, 0 Marker, 1 Sample 62.6mm 94ul
IB51085 1.5mm, 1 Marker, 1 Sample 4.7mm, 54.7mm 7.0ul, 82ul
6.) Make note of the total solution volume so that a degree of evaporation can be determined and
corrected for.
7.) Heat the agarose slurry in a microwave oven for 90 seconds. Swirl the flask to make sure any
grains sticking to the walls enter into the solution, undissolved agarose appears as small
"lenses" floating in the solution. Heat the solution for an additional 30-60 seconds.
Re-examine the solution and repeat the heating process until the agarose completely dissolves.
8.) Add deionized water to replace any volume lost through evaporation during the heating process.
Proceed to "Casting the Gel" on page 6.
PREPARATION OF THE AGAROSE GEL - RNA
RNA molecules are separated by electrophoresis through denaturing gels prior to analysis by northern
hybridization. Agarose gels containing formaldehyde are commonly used for RNA electrophoresis.
Presented below is a general protocol for electrophoresis of RNA using formaldehyde gels.
CAUTION! All equipment and solutions used in the following protocol should be treated with DEPC
(diethyl pyrocarbonate) or acetic anhydride prior to use to inhibit RNase activity. It is
recommended that dedicated solutions be made solely for RNA work to minimize the risk
of sample degradation due to RNase activity.
NOTE: Staining RNA samples with ethidium bromide has been reported to reduce sample blotting
efficiency. Therefore, if samples are to be analyzed by northern hybridization after elec-
trophoresis, run a duplicate lane(s) for staining, or minimize the exposure of RNA samples
to ethidium bromide by following the post-electrophoresis staining protocol on page 10.

6
The following protocol will make 50ml of a 1.5% agarose gel containing 1X MOPS [3-(N-
Morpholino)-Propanesulfonic Acid]-Acetate-EDTA (MAE) buffer and 2.2M formaldehyde, result-
ing in a 7.5mm thick gel:
1.) Weigh out 0.5gm of agarose, and place into a 125ml flask.
2.) Add 43.5ml of DEPC (or acetic anhydride) treated water.
3.) Make note of the total solution volume so that degree of evaporation can be determined and
corrected for.
4.) Heat the agarose slurry in a microwave oven for 60 seconds. Swirl the flask to make sure any
grains sticking to the walls enter into the solution. Undissolved agarose appears as small
"lenses" floating in the solution. Heat for an additional 30-60 seconds. Re-examine the solution
and repeat the heating process until the agarose completely dissolves.
5.) Add deionized water to replace any volume lost through evaporation during the heating process.
6.) Allow the solution to cool to 60oC. Place the flask in a hood and add 5ml of 10X MAE buffer,
and 1.5ml of 37% formaldehyde.
CAUTION: Formaldehyde vapors are toxic. Gel preparation should take place in a hood and solu-
tions and gels containing formaldehyde should be kept covered when possible.
CASTING THE GEL
1.) Place the gel casting fixture on a lab bench. Check
to see that it is level by placing the supplied level-
ing bubble in the center of the platform. The bub-
ble should appear within the center circle.
CAUTION! Cast agarose gels containing
formaldehyde in a hood.
2.) Insert the gel casting tray into the casting fix-
ture by pressing one end of the tray against the
thick foam backed casting pad. While com-
pressing the foam pad with the casting tray,
move the other end of the tray down into the
casting fixture (see Photo 1).
3.) When the gel solution has cooled to approxi-
mately 55oC, slowly pour it into the gel tray. If
hotter gel solutions are routinely poured, the
tray may warp over time.
4.) If bubbles form on the surface of the gel upon
pouring, use the comb to either pop them or lightly
brush them to the sides of the gel. If large bubbles
are allowed to harden within the gel, they may
cause artifacts to occur during electrophoresis.
5.) Insert one or more combs by placing them into the
slots in the casting tray. For best results, place the
comb in the slot nearest the thin pad of the casting
fixture. If two combs are desired, place the second
in the center comb slot (see Photo 2).
6.) Allow the gel to harden undisturbed for at least
30 minutes.
Photo 1
Photo 2

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REMOVING THE COMB
1.) When the gel is solidified and fully opaque, carefully remove the comb with a gentle wiggling,
upward motion. If the comb is difficult to remove or if a low percentage gel is being used,
overlay the comb area with a small volume of 1X electrophoresis buffer to preserve the integri-
ty of the wells. Check the wells to ensure their bases are intact.
CAUTION: Prolonged exposure of the Delrin combs to gels containing formaldehyde will cause
them to degrade. Be sure to remove the comb(s) from formaldehyde gels as soon as
gel hardening is complete and rinse them well prior to storage.
If a gel is not to be used immediately after preparation, remove it from the casting fixture and place
it in a plastic bag or container submerged in 1X electrophoresis buffer containing 1mMNaN3. Store
at +4oC.
LOADING THE SAMPLES INTO THE GEL
1.) Remove the casting tray containing the hard-
ened agarose gel from the casting fixture by
pressing the casting tray against the foam pad
and lifting at the thin pad end. Place the tray
and gel into the main unit assembly such that
the samples wells are on the same end as the
negative (black) electrode. (see Photo 3)
2.) Fill the unit with the remaining 1X elec-
trophoresis buffer containing ethidium bro-
mide made previously (or 1X MAE buffer
for RNA gels), covering the gel to a depth of
1-5mm. Approximately 250ml of buffer will
be required.
NOTE: Use of the same batch of electrophoresis
buffer for both the gel and the running
buffer is very important. Slight variations in buffer composition between gel and running
buffer may result in ionic or pH gradients that can significantly impact the mobility of
the samples.
3.) Pre-run RNA gels at 100V for five minutes prior to loading the samples.
4.) Load the samples into the wells with a micropipette or similar device taking care not to punc-
ture the bottom of the wells or load the sample onto the top of the gel. For improved well visu-
alization during sample loading, be sure that the wells are positioned over the contrasting
stripes located on the bottom of the buffer tanks.
Photo 3

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ELECTRICAL CONNECTIONS TO THE SAFETY LID
CAUTION: This unit is intended to be used with a power supply which detects a no current con-
dition and prevents a current flow unless there is a completed circuit path. Use of
other power supplies may compromise the safety of this unit.
The QS-710 can only be operated with the safety lid in place. Electrical current is supplied through
the banana plugs to the lid which, in turn, connect to the gold plated tank connectors. A simple
gravity connector in the cover ensures a complete current path, yet allows the lid to be removed
from the unit without disturbing the loaded samples.
1.) Plug the female ends of the black (-) and red
(+) leads into the banana jacks on the rear of
the lid. Be certain that the black (-) plug is
connected to the cathode end, where the sam-
ples originate (see Photo 4).
2.) After the samples have been loaded into the gel,
place the lid over the unit so that the connector
covers align with the round tank connectors.
3.) Set the lid straight down so that the lid rests
squarely on the unit and the round tank connec-
tors are inside the connector covers.
4.) Make sure the power supply is turned off. Plug
the male ends of the red and black leads into a
corresponding set of jacks on the power supply
(black - black; red - red).
CAUTION: Do not jar or bump the gel box once the lid is place. The electrical connection is
made by gravity once the lid is in position. While this design helps to minimize sam-
ple disturbance during lid placement, it also may result in a disruption of power to
the unit if the lid or unit are disturbed during the run.
SAMPLE ELECTROPHORESIS
1.) The QS-710 is designed for quick screen electrophoresis. The maximum suggested applied
voltage for the electrophoresis of DNA in agarose gels using the QS-710 is 100V. In a 1% TBE
gel, this translates into a run time of approximately 1/2 hour. Lower voltages may be used, of
course, and as a general rule, a 50V run will take twice as long as a 100V run. Higher voltages
may be used to decrease run time, however, if the unit is being operated at higher voltages than
100V, the heat generated during electrophoresis may decrease sample resolution. Such artifacts
may be avoided by running the unit in a cold room or adding 1X electrophoresis buffer "ice
cubes" to keep the unit properly cooled.
CAUTION: DO NOT EXCEED THE MAXIMUM OPERATING VOLTAGE OF 175 VOLTS.
The suggested run parameters for the electrophoresis of RNA in agarose gels containing formalde-
hyde is 60-80V. Since the QS-710 has no capacity for buffer recirculation, the buffer from each
reservoir should be collected after 1.5 hours of electrophoresis and properly discarded.
CAUTION: Formaldehyde vapors are toxic. Electrophoresis of RNA in gels containing formalde-
hyde should take place within a fume hood.
Photo 4

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2.) Follow the sample migration into the gel using the loading dye as an indicator. (See “Choice of
Buffer” for the Sample Loading Buffer recipe) Allow the samples to migrate until the fragments
have separated, normally until the bromophenol blue dye front has migrated 3/4 of the way down
the gel.
NOTE: If the gel contains ethidium bromide, the progress of electrophoresis may be monitored
during the run by turning off the power supply, removing the lid, and shining a medium-
wave UV light onto the gel. The resolved bands will appear as orange bands against a
dark purple background.
DETECTION AND DOCUMENTATION OF SEPARATED FRAGMENTS
1.) At the completion of the run, turn off the power supply and disconnect the leads. Remove the
lid (it is not necessary to remove the power leads from the lid) and remove the gel tray.
2.) To stain RNA gels containing formaldehyde post electrophoresis, soak the gel in 1L of DEPC-
treated water overnight at room temperature. Transfer the gel to a solution of 20X SSC contain-
ing 0.5μg/ml of ethidium bromide, stain for 5 -10 minutes.
3.) Ethidium bromide stained samples are visualized by exposing them to medium wavelength
(312nm) UV light. Because the gel casting tray is UV transmittant, the gel does not need to be
removed from the tray before viewing. Place the gel casting tray containing the gel on the filter
surface of a UV transilluminator for convenient viewing.
4.) Sample banding patterns may be documented by autoradiography.
CHOICE OF BUFFER
NOTE: Tris-borate buffer allows faster sample migration than tris-acetate buffers with no apparent
loss of resolution. A tris-borate buffer is usually the choice for quick-screening. Phosphate
buffers are used with glyoxal or formaldehyde gels.
The two most commonly used buffers for horizontal electrophoresis of double stranded DNA in
agarose gels are Tris-Acetate-EDTA (TAE) and Tris-Borate-EDTA (TBE). While the resolving
powers of these buffers are vary similar, the relative buffer capacities are very different, conferring
different run attributes which are summarized below:
Tris-Acetate has traditionally been the more commonly used buffer. However, its relativity low
buffer capacity will become exhausted during extended electrophoresis, making buffer recircula-
tion necessary in rums exceeding 140mA-hours. Potential advantages of using TAE buffer over
TBE buffer include superior resolution of supercoiled DNA and approximately 10% faster migra-
tion of the double-stranded linear DNA fragments.
Tris-Borate has a significantly greater buffering capacity and its relativity low current draw elimi-
nates the need for recirculation in all but the most extended runs (>300mA-hours). TBE buffer sys-
tems are not recommended when fragments are to be recovered from the gel after electrophoresis.
TRIS ACETATE EDTA BUFFER (TAE) - IB70160:
1X Working Concentration: 10X Stock Solution:
40 mM Tris base 48.4 g Tris Base
20 mM Glacial Acetic Acid (NaOAc) 16.4 g or 11.42ml NaOAc
2.0 mM EDTA 7.4 g EDTA or 20ml 0.5M EDTA (pH 8.0)
pH 8.3 H2O to 1L

10
TRIS BORATE EDTA BUFFER (TBE) - IB70150:
1X Working Concentration: 10X Stock Solution:
89 mM Tris Base 108g Tris Base
89 mM Boric Acid 55g Boric Acid
2.0 mM EDTA 6.72g EDTA or 40ml 0.5M EDTA (pH 8.0)
pH 8.0 H2O to 1 liter
MOPS ACETATE EDTA (MAE) - IB70175:
Solutions containing MOPS should be wrapped in aluminum foil and stored at room temperature.
The buffer tends to yellow with age. Light yellow buffer may be used, however, dark yellow solu-
tions should be discarded.
1X Working Concentration: 10X Stock Solution:
20 mM MOPS (pH 7.0) 41.8g MOPS
8 mM NaOAc 800 ml DEPC treated H2O
1 mM EDTA (pH 8.0) adjust pH to 7.0 with NAOH and add:
16.6ml 3M DEPC-treated NaOAc
20.0ml 0.5 M DEPC-treated EDTA, pH 8.0
bring to 1.0 liter and filter
Solutions containing MOPS should be wrapped in aluminum foil and stored at room temperature.
The buffer tends to yellow with age. Light yellow buffer may be used, however, dark yellow solu-
tions should be discarded.
Sample Loading Buffer - DNA Sample Loading Buffer - RNA
10X Stock Solution: 5X Stock Solution:
50 % Glycerol 1 mM EDTA, pH 8.0
100mM Na3EDTA 0.25 % Bromophenol Blue
1% SDS 0.25 % Xylene Cyanol
0.1% Bromophenol Blue 50 % Glycerol
pH 8.0
VOLTAGE
The QS-710 is designed for rapid electrophoresis with moderate resolution. Suggested voltage is
100V for a 30 to 60 minute run. Higher voltages may be used to decrease run time, however, the
volt-hours should remain constant. One should not exceed 200 volt-hours without changing the
buffer in the unit. If the unit begins to operate at voltages higher than 150V we recommend adding
1X electrophoresis buffer ice cubes to keep the unit properly cooled.
STAINING SOLUTION
The simplest staining procedure is to add 1-5g Ethidium Bromide per ml of gel solution just prior
to casting the gel. Alternatively, the gel can be stained in a solution of 5uG ethidium bromide/ml of
1X gel buffer for 15 minutes. Destain in deionized water or 1mM MgSO4for two minutes.

11
E. MAINTENANCE OFUNIT
Care must be observed in the handling of this unit.
DO NOT expose the unit to temperatures above 60°C
DO NOT expose the unit to organic solvents
DO NOT clean the unit with abrasive cleaners or cleaning aids.
Use mild cleaning solution (dish soap recommended) for routine cleaning. For heavier dirt, hand wash
with soft cloth. In most cases, a rinse in deionized water is sufficient to clean the unit. To remove
residual Ethidium Bromide from the gel unit, soak occasionally in 1% commercial bleach solution for
16 hours, and rinse well.
NOTE: The degradation of acrylic by solvents may result in substantial discoloration, cracking,
warpage or etching of the electrophoresis unit. DO NOT apply any of the following solvents
to the unit: benzene, xylene, toluene, chloroform, carbon tetrachloride, alcohol, phenol,
ketones, or esters. Do not use the Delrin combs supplied with this unit in formaldehyde for
long periods of time. The formaldehyde damages these combs with long exposures.
If an electrode breaks, contact Technical Support and Information Services at [phone] for an
electrode replacement.
ELIMINATION OF RNASE CONTAMINATION
Should treatment of the unit to eliminate RNase contamination be desired, clean the unit with a mild
detergent as described above, followed by soaking for 10 minutes in a solution of 3% hydrogen per-
oxide and then 1 hour in 0.1% DEPC (diethyl pyrocarbonate). Pour out final rinse and air dry.
CAUTION: DEPC is a suspected carcinogen, handle with care.
Alternatively, soak the unit and accessories in freshly made 2.2mM acetic anhydride treated water
(200ul/liter) for at least five minutes. Solutions for RNA work (electrophoresis buffers, etc.) may be
made from the same acetic anhydride treated water as well.
F. REPLACEMENT PARTS & ACCESSORIES
QS-710 ACCESSORY ITEMS AND REPLACEMENT PARTS:
Catalog # Description
IB50500 Replacement Power Cords
IB50504 Replacement Bubble Level
IB50505 Replacement Tank Connector Kit
IB51020 QS-710 UV Transmittant Casting Tray
IB51025 QS-710 Casting Fixture
IB51030 QS-710 UVT Casting Tray with Casting Fixture
IB51200 Replacement QS-710 Buffer Tank
IB51300 Replacement QS-710 Lid
IB51305 Replacement QS-710 Electrode Assembly

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QS-710 COMBS Sample Volume
Catalog # Description Well Width per mmGel
IB51040 Analytical Comb, 1.0mm x 8 tooth 4.7mm 4.7ul
IB51045 Analytical Comb, 1.0mm x 12 tooth 2.8mm 2.7ul
IB51050 Analytical Comb, 1.0mm x 15 tooth 2.2mm 2.3ul
IB51055 Analytical Comb, 1.5mm x 8 tooth 4.7mm 7.0ul
IB51060 Analytical Comb, 1.5mm x 12 tooth 2.8mm 4.0ul
IB51065 Analytical Comb, 1.5mm x 15 tooth 2.2mm 3.3ul
IB51046 Analytical Comb, 2.0mm x 12 tooth 3.0mm 6.0ul
IB51090 Analytical Comb, 2.0mm x 10 tooth 4.0mm 8.0ul
IB51095 Analytical Comb, 3.0mm x 6 tooth 5.8mm 17.3ul
IB51070 Preparative Comb, 1.0mm, 0 Marker, 1 Sample 62.6mm 62.6ul
IB51075 Preparative Comb, 1.0mm, 1 Marker, 1 Sample 4.7mm, 54.7mm 4.7ul, 54.7ul
IB51080 Preparative Comb, 1.5mm, 0 Marker, 1 Sample 62.6mm 94.0ul
IB51085 Preparative Comb, 1.5mm, 1 Marker, 1 Sample 4.7mm, 54.7 7.0ul, 82.0ul
G. RELATED IBI PRODUCTS
IB50000 IBI QSH Lab-Pal (5 X 7cm Horizontal Electrophoresis Unit)
Comes complete with buffer tank, vented lid, 2-place casting tray, two 1.5mm by
5-tooth combs, four glass slide, power cords, and manual.
IB53000 IBI MP-1015 (10 X 15cm Horizontal Electrophoresis Unit)
Comes complete with buffer tank, vented lid, casting fixture and UVT tray, two 2.0mm
by 16-tooth combs, power cords, buffer port set, leveling bubble and manual.
IB56000 IBI HR-2025 (20 X 25cm Horizontal Electrophoresis Unit)
Comes complete with buffer tank, vented lid, casting fixture and UVT tray, two 2.0mm
by 20-tooth combs, power cords, buffer port set, leveling bubble and manual.
IB57000 IBI HR-2525 (25 X 25cm Horizontal Electrophoresis Unit)
Comes complete with buffer tank, vented lid, casting fixture and UVT tray, four 2.0mm
by 50-tooth combs, power cords, buffer port set, leveling bubble and manual.
IB62000 IBI VCV Vertical Electrophoresis System (18 X 22cm Vertical Electrophoresis Unit)
Comes complete with main assembly, safety cover, three glass plates (inner, outer, and
frosted), one 1.5mm by 12-tooth and 1.5mm by 20-tooth combs, a 1.5mm spacer set
(which includes one bottom and two sided spacers as well as two spacer tabs), one set
of power cords, four sandwich clips, and manual.
IB80000 IBI STS-45i Manual Sequencer (36 X 43cm Vertical Electrophoresis Unit)
Comes complete with main assembly, aluminum thermoplate, two glass plates, one
0.4mm comb and spacer set (includes two 32-tooth and 64-tooth conventional combs,
two 64-tooth sharkstooth combs, one bottom and two sided spacers and four spacer
tabs) one set of power cords, and manual.
IB94000 IBI MaGELin Universal Protein System (for Cast-Your-Own or Precast Gels)
Comes complete with buffer tank, gel capture device, vented lid, vertical casting
fixture, two sets of 0.8mm side spacers, three outer glass plates, three inner notched
plates, two 0.8mm by 12-tooth combs, power cords, and manual
SH-300 IBI 300V Power Supply (300V / 400mA / 120W) The SH-300 has constant voltage or
constant current capability, memory settings, and a LED display. Comes complete with
power supply, 120V grounded power cord, and manual.
SH-500 IBI 500V Power Supply (500V / 300mA / 150W) The SH-500 has constant voltage
or constant current capability, memory settings, gel saver feature, and a LED display.
Comes complete with power supply, 120V grounded power cord, and manual.

13
H. RELATED IBI CERTIFIED REAGENTS
IB01010 6X Loading Dye 5ml
IB01015 5X RNA Gel Loading Dye Kit 100RxN
IB01020 10X TBE Pouch 1 Pouch
IB01030 25X Tris-Acetate EDTA Buffer Pouch 1 Pouch
IB74020 Acridine Orange 25gm
IB70016 Acrylamide:Bisacrylamide, 29:1 40gm
IB70017 Acrylamide:Bisacrylamide, 29:1 200gm
IB70020 Acrylamide 100gm
IB70022 Acrylamide:Bisacrylamide, 19:1 40gm
IB70023 Acrylamide:Bisacrylamide, 19:1 200gm
IB70024 Acrylamide 500gm
IB70026 Acrylamide 1.5kg
IB70028 Acrylamide 3kg
IB70018 Acrylamide:Bisacrylamide, 37.5:1 40gm
IB70019 Acrylamide:Bisacrylamide, 37.5:1 200gm
IB70010 Acryliqud-40 (40% (w/v) Acrylamide solution) 500ml
IB70035 Agarose 25gm
IB70040 Agarose 100gm
IB70041 Agarose 250gm
IB70042 Agarose 500gm
IB70045 Agarose 1kg
IB70050 Agarose, Low Melting Point 50gm
IB70051 Agarose, Low Melting Point 25gm
IB70056 Agarose, Low Melting Point 100gm
IB70057 Agarose, Low Melting Point 250gm
IB70058 Agarose, Low Melting Point 500gm
IB70059 Agarose, Low Melting Point 1Kg
IB70052 3:1 Super Sieve Agarose 50gm
IB70053 3:1 Super Sieve Agarose 250gm
IB70054 Ultra Sieve Agarose 25gm
IB70055 Ultra Sieve Agarose 250gm
IB70060 Agarose, PFGE 25gm
IB70061 Agarose, PFGE 50gm
IB70062 Agarose, PFGE 100gm
IB70063 Agarose, PFGE 250gm
IB70064 Agarose, PFGE 500gm
IB70065 Agarose, PFGE 1Kg
IB15720 Alcohol-Anhydrous (Ethanol) 500ml
IB15721 Alcohol-Anhydrous (Ethanol) 1L
IB15724 Alcohol-Anhydrous (Ethanol) 4L
IB15620 Ammonium Acetate 500gm
IB70080 Ammonium Persulfate 100gm
IB02040 Ampicillin, Sodium Salt 25gm
IB70100 Bisacrylamide 25gm
IB70102 Bisacrylamide 100gm
IB70096 Boric Acid 2.5kg
IB74040 Bromophenol Blue 25gm
IB02010 Carbenicillin 1gm
IB02020 Carbenicillin 5gm
IB37060 Cesium Chloride, Optical Grade 100gm
IB37062 Cesium Chloride, Optical Grade 1kg
IB37042 Cesium Chloride, Technical Grade 1kg
IB02080 Chloramphenicol 25gm
IB05040 Chloroform 500ml

14
IB21040 Dithiothreitol (DTT) 5gm
IB21045 Dithiothreitol (DTT) 25gm
IB70180 EDTA, disodium salt 100gm
IB70182 EDTA, disodium salt 500gm
IB70184 EDTA Solution (0.5M), pH 8 100ml
IB70185 EDTA Solution (0.5M), pH 8 4x100ml
IB40060 Ethidium Bromide 5gm
IB40075 Ethidium Bromide Solution, 10mg/mL 10ml
IB72028 Formamide, ACS Grade 500ml
IB72020 Formamide, Spectral Grade 100ml
IB72024 Formamide, Spectral Grade 500ml
IB02030 Gentamycin Solution 20ml
IB15760 Glycerol 500ml
IB15762 Glycerol 1L
IB70194 Glycine 2.5kg
IB05080 Guanidine Hydrochloride 500gm
IB05085 Guanidine Hydrochloride Solution (6M) 500ml
IB05100 Guanidine Thiocyanate 500gm
IB01120 HEPES, Sodium Salt 100gm
IB01130 HEPES, Free Acid 50gm
IB01131 HEPES, Free Acid 250gm
IB01132 HEPES, Free Acid 500gm
IB01133 HEPES, Free Acid 1Kg
IB70012 InstaBIS-(2% (w/v) Bisacrylamide solution) 500ml
IB70000 InstaPAGE-(30% sol., 19:1 Acrylamide:Bisacrylamide) 500ml
IB70001 InstaPAGE-(30% sol., 19:1 Acrylamide:Bisacrylamide) 1L
IB70002 InstaPAGE-(30% sol., 29:1 Acrylamide:Bisacrylamide) 500ml
IB70003 InstaPAGE-(30% sol., 29:1 Acrylamide:Bisacrylamide) 1L
IB70004 InstaPAGE-(30% sol., 37.5:1 Acrylamide:Bisacrylamide) 500ml
IB70005 InstaPAGE-(30% sol., 37.5:1 Acrylamide:Bisacrylamide) 1L
IB70006 InstaPAGE-(40% sol., 29:1 Acrylamide:Bisacrylamide) 500ml
IB70007 InstaPAGE-(40% sol., 29:1 Acrylamide:Bisacrylamide) 1L
IB70008 InstaPAGE-(40% sol., 37.5:1 Acrylamide:Bisacrylamide) 500ml
IB70009 InstaPAGE-(40% sol., 37.5:1 Acrylamide:Bisacrylamide) 1L
IB70014 InstaPAGE-(40% sol., 19:1 Acrylamide:Bisacrylamide) 500ml
IB70015 InstaPAGE-(40% sol., 19:1 Acrylamide:Bisacrylamide) 1L
IB02100 IPTG 1gm
IB02105 IPTG 5gm
IB02125 IPTG 25gm
IB05120 Isobutanol 500ml
IB15730 Isopropanol 500ml
IB15735 Isopropanol 1L
IB02120 Kanamycin Sulfate 25gm
IB15750 Methanol - HPLC Grade 1L
IB15755 Methanol - Ultra Pure Grade 500ml
IB15756 Methanol - Ultra Pure Grade 1L
IB15757 Methanol - Ultra Pure Grade 4L
IB74050 Methylene Blue, Chloride, trihydrate 25gm
IB70170 MOPS 100gm
IB70175 MOPS Decp, 10X 100ml
IB05160 Phenol - Crystalline 100gm
IB05164 Phenol - Crystalline 500gm
IB05174 Phenol Chloroform Solution 400ml
IB05182 Phenol, Buffer Saturated, pH 6.6-8.0 100ml
IB05184 Phenol, Buffer Saturated, pH 4.3 100ml
IB05400 Proteinase K 100mg
IB05406 Proteinase K Solution (20mg/mL) 5ml
IB07080 Sarkosyl 100gm

15
IB07060 Sodium Dodecyl Sulfate (SDS) 100gm
IB07062 Sodium Dodecyl Sulfate (SDS) 500gm
IB07064 Sodium Dodecyl Sulfate (SDS) Solution, 20% 100ml
IB72010 SSC (20X)-Nucleid Acid Prep and Blotting Solution 1L
IB72015 SSPE (20X) - Nucleid Hybridization Solution 1L
IB02180 Streptomycin Sulfate 25gm
IB37160 Sucrose 1kg
IB70120 TEMED 50gm
IB02200 Tetracycline Hydrochloride 25gm
IB70142 Tris 500gm
IB70144 Tris 1kg
IB70145 Tris 5kg
IB70150 Tris Borate EDTA (10X TBE Buffer) 1L
IB70153 Tris Borate EDTA (10X TBE Buffer) 4L
IB70154 Tris Borate EDTA (10X TBE Buffer) 10L
IB70155 Tris Borate EDTA (20X Modified TBE Buffer) 1L
IB70160 Tris Acetate EDTA (10X TAE) Buffer 1L
IB70162 Tris-Hydrochloride 500gm
IB07100 Triton X-100 100ml
IB72060 Urea 500gm
IB72064 Urea 2.5kg
IB02260 X-GAL 1gm
IB02264 X-GAL 100mg
IB72120 Xylene Cyanol FF 25gm
I. REFERENCES
1.) Lehrach, H., et al. 1977. Biochemistry 16:4743.
2.) Sambrook, J., Fritsch, E.F., and Maniatis, T., (1989). Molecular Cloning, A Laboratory Manual,
volume 1. Cold Spring Harbor Press, New York.
3.) Selden, R.F. (1988) Analysis of RNA by Northern Hybridization," in Current Protocols in
Molecular Biology, F.M. Ausubel, et. al, editors, volume 1, p.4.9.1. Green Publishing Associates
and Wiley-Interscience.
J. LIMITED WARRANTY
Our limited warranty for all electrophoresis gel boxes is four (4) years to the original buyer only (non-
transferable). Warranty does not apply to electrodes or platinum wires.
Our limited warranty as noted above extends to the direct end user of IBI Scientific products only.
This warranty is in lieu of all other warranties whether expressed or implied, including warranties of
merchantability or fitness for a particular purpose. In no situation shall IBI Scientific be liable for any
incidental or consequential damages of any kind, even though IBI Scientific has been advised of the pos-
sibility of such damages arising out of, or resulting from, the products or the use or modification thereof
or due to the breach of this warranty or any other obligation of IBI Scientific to the customer, whether
based on contract, tort, or any other legal theory. In no such event shall IBI Scientific be liable for dam-
ages which exceed the purchase price of any products.