Invitrogen EVOS M5000 User manual

For Research Use Only. Not for use in diagnostic procedures.
EVOS™M5000 Imaging System
USER GUIDE
For Fluorescence and Transmitted Light Applications
Catalog Number AMF5000 and AMF5000SV
Publication Number MAN0017563
Revision F.0

Life Technologies Corporation | 22025 20th Ave SE Ste. 100 | Bothell, Washington 98021 USA
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
Revision history: MAN0017563 F.0 (English)
Revision Date Description
F.0 16 November
2023 Add Stage View tool and update screens and content.
E.0 14 December
2022 Update Connect account sign in, add information about Align Channels tool, update relevant screens.
D.0 1 April 2021 Add information about Transfection Eciency calculation and Batch Analysis, update Appendix B - Review tab,
update the screens throughout the document.
C.0 14 January 2020 Add information about adjustable scale bar, Clear All button for images in the memory buer, Hot Pixel Correction,
Z-Stack infographics, additional objective data, additional metadata for PNG and TIFF images, Time/Date setup,
automatic reconnect to WiFi networks and mapped network drives, new authentication code requirement for
first-time Connect account connection for added login security, and update the screens throughout the document.
B.0 8 January 2019 Add Connect account sign in and save, Confluence tool, Time Lapse video save, review, and playback, EVOS™
Onstage Incubator, and EVOS™Image Analysis sections, update GUI section and the screens throughout the
document.
A.0 7 August 2018 New user guide for the EVOS™M5000 Imaging System.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2018-2023 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER1About thisguide ...................................................... 9
Audience ....................................................................... 9
User attention words ............................................................ 9
Safety alert words ............................................................... 9
■CHAPTER2Productinformation ................................................ 10
Productdescription ............................................................ 10
EVOS™M5000 Imaging System ............................................. 10
EVOS™M5000 Software .................................................... 10
Productuse ............................................................... 11
Standard itemsincluded ........................................................ 11
EVOS™M5000 Imaging System ............................................. 11
EVOS™M5000 AccessoriesKit .............................................. 11
EVOS™M5000 Imaging System userdocumentation ........................... 12
Instrument exterior components and mechanical controls ........................... 13
Frontview ................................................................ 13
Rearview ................................................................. 14
Graphical user interface(GUI) .................................................... 15
GUIlayout ................................................................ 15
■CHAPTER3Installation ........................................................... 16
Operating environment and site requirements ...................................... 16
Cell culture hoodsetup ..................................................... 16
Prepare forinstallation .......................................................... 17
Receive and inspect theshipment ........................................... 17
Move the instrument to the installation site .................................... 17
Install theinstrument ........................................................... 17
Unpack theinstrument ..................................................... 17
Remove shipping restraints ................................................. 18
Install the UV lightshield .................................................... 20
Connect theinstrument ..................................................... 22
EVOS™M5000 Imaging System User Guide 3

Turn on the EVOS™M5000 Imaging System ....................................... 22
Connect the instrument to the internet ........................................ 22
Set date andtime .......................................................... 22
Connect to the Thermo Fisher™ConnectPlatform .................................. 23
About the Thermo Fisher™ConnectPlatform .................................. 23
Create a Connectaccount .................................................. 23
Create a PINnumber ....................................................... 23
Link instrument to your Connectaccount ..................................... 24
Add instrument to your Connect account with QR code (MobileDevice) .......... 25
Add instrument to Thermo Fisher™Connect Platform with linking code(PC) ....... 25
Sign in to your Connect account from the EVOS™M5000instrument ............. 25
Set up a new administrator .................................................. 26
■CHAPTER4Captureimages ..................................................... 27
Overview ...................................................................... 27
Workflow ................................................................. 27
Capturetab ............................................................... 28
Capture images in a singlechannel ............................................... 28
Select objective and light source ............................................ 28
Adjustbrightness .......................................................... 29
Focus on thesample ....................................................... 30
Optional: Adjust displaysettings ............................................. 31
Find a region of interest .................................................... 31
Capture images for eachchannel ............................................ 32
Capture images in multiplechannels ............................................. 34
Capture multiple channels automatically ...................................... 34
■CHAPTER5Measure, annotate, and analyze capturedimages .............. 36
Display settings and analysis tools ............................................... 36
Adjust image displaysettings .................................................... 37
Displaygrid ................................................................... 38
Display scalebar ............................................................... 39
Alignchannels ................................................................. 40
Alignchannels ............................................................. 40
View pixel intensity histogram ................................................... 41
Display histogram .......................................................... 41
Add measurements andannotations ............................................. 42
Analyze cell culture ............................................................. 44
Analysis tools ............................................................. 44
Count cells – autocount ........................................................ 45
Perform autocount ........................................................ 45
Contents
4EVOS™M5000 Imaging System User Guide

Count cells – manualcount ...................................................... 51
Perform manualcount ...................................................... 51
Measureconfluence ............................................................ 54
Confluence tool ........................................................... 54
Calculate transfectioneciency ............................................. 58
■CHAPTER6Save capturedimages .............................................. 62
Save ......................................................................... 62
Save imagesmanually ...................................................... 62
Quick Saveimages ............................................................. 63
(Optional) Enable QuickSave ................................................ 63
■CHAPTER7Capture time lapseimages ........................................ 65
Time lapse tool ................................................................ 65
Run a time lapse routine ........................................................ 65
Run a new time lapse routine ................................................ 65
Run a saved time lapse routine .............................................. 70
Review images and video captured in a time lapse routine ...................... 71
■CHAPTER8Capture Z-Stack .................................................... 74
Z-Stack tool ................................................................... 74
Capture Z-stackimages ........................................................ 74
■CHAPTER9Stage View .......................................................... 76
Overview ...................................................................... 76
Workflow ................................................................. 77
Set StageOrigin ............................................................... 78
Mark pinlocations ............................................................. 81
Find pins using the Find Target screen ............................................ 81
Load PinMap ................................................................. 84
Update the target pinlocation ................................................... 84
■CHAPTER10Review and analyze savedimages .............................. 85
Reviewimages ................................................................ 86
Reviewimages ............................................................ 86
Configure displaysettings ....................................................... 88
Adjust display settings for savedimages ...................................... 88
Displaygrid ............................................................... 89
Display scalebar .......................................................... 89
View pixel intensity histogram ................................................... 89
Display histogram .......................................................... 89
Contents
EVOS™M5000 Imaging System User Guide 5

Add measurements and annotations to savedimages .............................. 90
Analyze cell culture using savedimages .......................................... 90
Save analysis results ........................................................... 92
Save ..................................................................... 92
Batchanalysis ................................................................. 94
Batch Analysisfunction ..................................................... 94
Save current analysissettings ............................................... 94
Run batchanalysis ......................................................... 94
■CHAPTER11Configure instrumentsettings .................................... 96
Overview ...................................................................... 96
Settingstab ............................................................... 96
Adjust objectivesettings ........................................................ 98
Assignobjectives .......................................................... 98
Calibrate objectivemagnification ............................................ 99
Deleteobjectives .......................................................... 99
Calibrate whitebalance ........................................................ 100
Calibrate whitebalance .................................................... 100
Set saturated pixeloptions ..................................................... 101
Highlight saturated pixels .................................................. 101
Generalsettings .............................................................. 101
General settingsoptions ................................................... 101
Define Capture All checkboxbehavior ....................................... 101
Define Display Settings toolbarbehavior ..................................... 102
Show Align Channels tool .................................................. 102
Configure networksettings ..................................................... 102
Connect to a Wi-Finetwork ................................................ 103
Map networkdrive ........................................................ 103
Service ...................................................................... 105
Copy errorlogs ........................................................... 105
Set date andtime ......................................................... 105
Update fromcloud ........................................................ 106
Update fromUSB ......................................................... 106
■CHAPTER12Instrument care and maintenance .............................. 107
General care ................................................................. 107
Objective lens care ............................................................ 108
Stage care ................................................................... 108
Decontamination procedures ................................................... 108
Change EVOS™LED lightcubes ................................................ 109
Change LED lightcube .................................................... 109
Contents
6EVOS™M5000 Imaging System User Guide

Change theobjectives ......................................................... 110
Procedure for objectivechange ............................................ 110
Calibrate theobjectives ........................................................ 112
Calibrate objectivemagnification ........................................... 112
■APPENDIXATroubleshooting .................................................. 114
Image qualityissues ........................................................... 114
Stage viewissues ............................................................. 115
Software interfaceissues ...................................................... 115
Mechanicalissues ............................................................ 116
■APPENDIXBGraphical user interface(GUI) ................................... 117
Capturetab .................................................................. 117
Reviewtab ................................................................... 126
Auto count controls ........................................................... 128
Manual count controls ......................................................... 129
Cell culture – confluence controls ............................................... 130
Cell culture – transfection eciency controls ..................................... 131
Settings ..................................................................... 132
■APPENDIXCSystem overview ................................................. 135
Technicalspecifications ........................................................ 135
Physical characteristics ................................................... 135
Hardware ................................................................ 135
Operation principles and technical overview ...................................... 136
LEDillumination .......................................................... 136
LED lightcubes .......................................................... 136
■APPENDIXDEVOS™imageanalysis ........................................... 138
EVOS™imageanalysis ......................................................... 138
Gallery ...................................................................... 139
Editimage ................................................................... 139
Adjusttab ............................................................... 140
Analyzetab .............................................................. 141
■APPENDIXEGlossary ........................................................... 142
Software controls andfunctions ................................................ 142
Imagefiles ................................................................... 143
Microscope controls andoptics ................................................. 145
Stage View feature ............................................................ 146
Contents
EVOS™M5000 Imaging System User Guide 7

■APPENDIXFSafety .............................................................. 147
Symbols on thisinstrument .................................................... 147
Standard safetysymbols .................................................. 147
Location oflabels ......................................................... 149
Control and connectionsymbols ........................................... 149
Conformitysymbols ...................................................... 149
Safety information for instruments not manufactured by Thermo FisherScientific ..... 150
Instrumentsafety ............................................................. 150
General ................................................................. 150
Physical injury ............................................................ 151
Electricalsafety .......................................................... 152
Cleaning anddecontamination ............................................. 153
Instrument component and accessorydisposal .............................. 153
Safety and electromagnetic compatibility (EMC) standards ......................... 153
Safety standards ......................................................... 154
EMC standards ........................................................... 154
Environmental design standards ............................................ 155
Chemicalsafety .............................................................. 156
Biological hazardsafety ....................................................... 157
■APPENDIXGDocumentation and support .................................... 158
Customer and technical support ................................................ 158
Limited product warranty ...................................................... 158
Contents
8EVOS™M5000 Imaging System User Guide

About this guide
Audience
This user guide is for laboratory sta operating, maintaining, and analyzing data using the Invitrogen™
EVOS™M5000 Imaging System.
User attention words
Two user attention words appear in this document. Each word implies a specific level of observation or
action as described below.
Note: Provides information that may be of interest or help but is not critical to the use of the product.
IMPORTANT! Provides information that is necessary for proper instrument operation, accurate
installation, or safe use of a chemical.
Safety alert words
Three safety alert words appear in this document at points where you need to be aware of relevant
hazards. Each alert word—CAUTION, WARNING, DANGER—implies a particular level of observation
or action, as defined below:
CAUTION! Indicates a potentially hazardous situation that, if not avoided, may result in minor or
moderate injury. It may also be used to alert against unsafe practices.
WARNING! Indicates a potentially hazardous situation that, if not avoided, could result in death or
serious injury.
DANGER! Indicates an imminently hazardous situation that, if not avoided, will result in death or
serious injury. This signal word is to be limited to the most extreme situations.
1
EVOS™M5000 Imaging System User Guide 9

Product information
Product description
EVOS™M5000 Imaging System
The Invitrogen™EVOS™M5000 Imaging System (Cat. No. AMF5000SV) is a fully integrated, digital,
inverted cell imaging system for four-color fluorescence and transmitted-light applications.
It combines precision optics, a five-objective turret, an 18.5 inch high-resolution LCD display (1920 x
1080 pixel resolution), a highly sensitive monochrome CMOS camera (2048 x 1536 pixel resolution, 3.2
Megapixels) to acquire images seamlessly through an intuitive user interface using a mouse for easy
control, and a position-sensing stage that enables sample locations to be mapped and stored for fast
future acquisition.
EVOS™M5000 Software
The EVOS™M5000 Imaging System is controlled by the integrated Invitrogen™EVOS™M5000 Software
through a graphical user interface (GUI), which is accessed by the computer mouse and keyboard. The
software is pre-installed and starts automatically when the instrument is powered on.
Key features of the EVOS™M5000 Software include:
•Stage View: Integrates a precision manual stage with sensors for accurately mapping and marking
points of interest in various vessel types, including plates, slides, and petri dishes.
Note: Stage View requires an updated stage manufactured in early 2024 for all new EVOS™
M5000 systems. Prior EVOS™M5000 systems using a stage with separate X- and Y-axis control
knobs will not have access to the Stage View feature but can be updated to have all of the other
software features described in this quick reference guide.
•Capture: Enables control over every aspect of image capture through a simple user interface. All
images acquired can be saved in JPG, PNG, and TIFF formats, or compiled into a video sequence
in AVI or MP4 formats.
•Autofocus: Enables autofocus in fluorescence and brightfield modes.
•Z-stacking: Captures a series of images along the z-axis that can be saved individually or
combined into a Z-stack projection with a greater depth of field than any of the individual source
images.
•Time lapse: Enables creating a time-lapse movie using captured images.
•Review: Enables reviewing, measuring, and annotating captured images.
•Cell count: Enables automatic or manual counting of cells post-acquisition.
•Confluence: Enables calculating the percentage confluence of the culture based on selected
reference objects and background.
2
10 EVOS™M5000 Imaging System User Guide

•Transfection Eciency: Enables measuring percent transfection in a population of cells, as
determined after measuring confluence.
•Network and Connect connectivity: Allows Wi-Fi and Ethernet connectivity to the network and to
your Connect account as part of the Connect-based platform to store and access your data files.
Product use
For Research Use Only. Not for use in diagnostic procedures.
Standard items included
EVOS™M5000 Imaging System
EVOS™M5000 Imaging System, includes the following components and pre-installed accessories:
•18.5 in articulated LCD monitor (1920 ×1080 pixel resolution)
•Embedded PC (4GB RAM)
•Manual X-Y stage with position-sensing technology
•5‑position objective turret
•Condenser with 4‑position turret
•Light cube shipping restraint (remove before use)
•Stage lock pin (remove before use)
•EVOS™Condenser Light Shield
•Light cube tool (remove before use)
•Blank light cube (remove before use)
•LED light cubes, as ordered
•Objectives, as ordered
EVOS™M5000 Accessories Kit
EVOS™M5000 Accessories Kit (located in the instrument box), contains:
•Wireless mouse and keyboard
•USB receiver (for wireless mouse and keyboard connection)
•USB Wi-Fi adaptor (for wireless network connection to Connect applications and mapped network
drives)
•USB 3.0 flash drive, 16 GB (for image storage, preloaded with user documentation)
•EVOS™Vessel Holder, Multiwell Plate (Cat. No. AMEPHV022)
•EVOS™Vessel Holder, Two 25 × 75 mm slides (Cat. No. AMEPVH001)
•EVOS™Vessel Holder, Universal (Cat. No. AMEPVH009)
•EVOS™Calibration Slide (Cat. No. AMEP4720)
•EVOS™Light Shield
•UV shield assembly
Chapter2Product information
Standard items included 2
EVOS™M5000 Imaging System User Guide 11

•Condenser Slider, Block (Cat. No. AMEP4688)
•Condenser Slider, 4X Pupil (Cat. No. AMEP4738)
•Condenser Slider, Diusion for Brightfield Applications (Cat. No. AMEPDFS1)
•Universal power supply (12 V, 5 A) and power cord (type B, North America)
•EVOS™Dust Cover
•Accessories box with adjustable compartments
•Hex key, 2mm
•Mouse pad
EVOS™M5000 Imaging System user documentation
•EVOS™M5000 Imaging System Quick Start Guide, printed (Pub. No. MAN0017765)
•EVOS™M5000 Imaging System Installation Guide, printed (Pub. No. MAN0017783)
•Pre-loaded to USB 3.0 flash drive (located in the accessories box):
–EVOS™M5000 Imaging System User Guide (Pub. No. MAN0017763)
–EVOS™M5000 Imaging System Quick Start Guide (Pub. No. MAN0017765)
–EVOS™M5000 Imaging System Installation Guide (Pub. No. MAN0017783)
Chapter2Product information
Standard items included
2
12 EVOS™M5000 Imaging System User Guide

Instrument exterior components and mechanical controls
Front view
1
2
3
54
6
11
8
8
7
10
9
1LCD monitor
2Condenser
3Condenser light shield
4Vessel holder and thumb screws
5Mechanical X-Y stage
6Stage X- and Y-axis positioning knobs
7Objective selection wheel
8Focusing knobs
9USB-A 3.0 port
10 Objective
11 Phase ring selector
Chapter2Product information
Instrument exterior components and mechanical controls 2
EVOS™M5000 Imaging System User Guide 13

Rear view
1LCD monitor
2Mechanical X-Y stage
3Focusing knobs
4USB-A 3.0 port (2×)
5Power switch
6Single-pin power input port (12VDC, 5A)
7DisplayPort (video output)
8Network port
9USB-A 2.0 ports (2×)
10 Stage X- and Y-axis positioning knobs
11 Instrument passwords sticker
Chapter2Product information
Instrument exterior components and mechanical controls
2
14 EVOS™M5000 Imaging System User Guide

Graphical user interface (GUI)
GUI layout
The GUI of the system consists of the Viewing area on the left and Capture and Review tabs and the
Settings and Virtual keyboard buttons on the right. Each tab and button opens the controls necessary
to execute the selected function.
1 2 3 4 56
1Sign In/User: Allows you to sign in to your Connect account, Connect-based platform, for image storage and
analysis with the EVOS™Image Analysis application.
2Viewing area: Displays the live or captured images of the sample.
3Capture tab: Contains the controls for image capture.
4Review tab: Allows you to review and annotate captured images.
5Keyboard button: Opens the virtual keyboard.
6Settings button: Opens the Settings tabs, which allow you to select and adjust basic and advanced system settings.
Note: For more information and detailed descriptions of GUI controls, see Appendix B, “Graphical user
interface (GUI)”.
Note: For more information on the EVOS™Image Analysis application, see Appendix D, “EVOS™image
analysis”.
Chapter2Product information
Graphical user interface (GUI) 2
EVOS™M5000 Imaging System User Guide 15

Installation
Operating environment and site requirements
•The dimensions of the EVOS™M5000 Imaging System are 18×23×18in (46×59×46cm)
(W × H × D). The system requires a benchtop of approximately 36×36in(92×92cm).
•If the system includes the optional EVOS™Onstage Incubator (Cat. No. AMC2000), then add 16in
(40 cm) to the width of the bench.
•Allow at least 2 in (5 cm) of free space at the back of the instrument to allow for proper ventilation
and prevent overheating of electronic components.
•Place the EVOS™M5000 Imaging System on a level surface away from vibrations from other pieces
of equipment. Tabletop centrifuges, vortex mixers, and other laboratory equipment can vibrate the
instrument during operation and interfere with instrument performance.
•If possible, install the EVOS™M5000 Imaging System away from direct light sources such as
windows. Ambient light can enter the imaging path and aect the image quality.
•Operating temperature range: 4°–32°C (40°–90°F).
•Relative humidity range: 0–90%.
•Operating power: 100–240 VAC, 1.8A
•Frequency: 50–60Hz
•Electrical input: 12VDC, 5A
IMPORTANT! Do not position the instrument so that it is dicult to turn o the main power switch
located on the back of the instrument base (see “Rear view” on page14). In case of an instrument
malfunction, turn the main power switch to the OFF position and disconnect the instrument from the
wall outlet.
Cell culture hood setup
The EVOS™M5000 Imaging System fits in cell culture hoods that are at least 24in (61cm) deep and
36 in (92 cm) high with a 30 in (76 cm) opening. If your cell culture hood is smaller, it may be possible to
fit the instrument by turning it at a slight angle.
3
16 EVOS™M5000 Imaging System User Guide

Prepare for installation
Receive and inspect the shipment
1. Verify that the items shown on the shipping list are the same items that you ordered at the time of
purchase.
2. Carefully inspect the shipping containers and report any damage to the Thermo Fisher Scientific
service representative. Record any damage or mishandling on the shipping documents.
Move the instrument to the installation site
1. Clear the installation site of all unnecessary materials.
2. If possible, move the crated instrument and other shipping containers to the installation site.
CAUTION! PHYSICAL INJURY HAZARD. Lift or move the instrument using proper lifting
techniques. We recommend that you lift or move the crated instrument with the assistance of others
and the use of appropriate moving equipment. Improper lifting can cause painful and permanent back
injury. Depending on the weight, moving or lifting an instrument may require two or more persons.
Install the instrument
Unpack the instrument
1. Open the shipping box and remove the accessory box.
2. Carefully lift the instrument out of the box by grasping it firmly with both hands under the support
arm.
3. Place the instrument on a flat, level surface that will be free from vibration and leave enough room
around it for the stage to move freely.
4. Tilt the LCD monitor upright.
5. Examine the instrument carefully for damage incurred during transit.
6. Unpack the accessories box and verify all parts are present. See “Standard items included” on
page11 for the list of standard items included in the shipment.
IMPORTANT! Do not subject the EVOS™M5000 Imaging System to sudden impact or excessive
vibration. Handle the instrument with care to prevent damage.
Note: Contact your distributor if anything is missing. If you do not have your distributor information,
contact Technical Support. Damage claims must be filed with the carrier; the warranty does not cover
in-transit damage.
Chapter3Installation
Prepare for installation 3
EVOS™M5000 Imaging System User Guide 17

Note: Make sure to set aside packaging and foam for future transport and storage. Re-install the stage
lock pin and the light cube shipping restraint before moving or transporting the instrument. Always
ensure that the instrument is properly cushioned and braced to prevent damage.
Remove shipping restraints
The EVOS™M5000 Imaging System is equipped with two shipping restraints (stage lock pin and light
cube shipping restraint) to protect the instrument from shock and vibration during transport. You must
remove the shipping restraints before you power on the EVOS™M5000 Imaging System.
2
3
1
Figure1Before removing the stage lock pin.
1Stage lock pin
2Light cube shipping restraint
3Light cube tool
1. Pull firmly to remove the stage lock pin.
2. Using the Y-axis stage positioning knob, move the stage back to obtain access to the light cube
shipping restraint, which is centered under the back of the stage.
Note: The light cube shipping restraint is secured with the light cube tool to the blank light cube
installed in the light cube turret. Used together, they immobilize the light cube turret to protect it
during transport.
Chapter3Installation
Install the instrument
3
18 EVOS™M5000 Imaging System User Guide

Figure2After removing the stage lock pin and moving the stage to access the shipping restraint.
3. Unscrew and remove the light cube tool, which secures the shipping restraint block to the blank
light cube.
4. Remove the shipping restraint block and store it in the accessories box. Removal of the restraint
block provides access to the blank light cube in the light cube turret.
Note: The blank light cube is red and does not have the grooved copper top of the LED light
cubes.
1Blank light cube
2Thread hold
3Screws
Chapter3Installation
Install the instrument 3
EVOS™M5000 Imaging System User Guide 19

5. Using the light cube tool, loosen the two screws that secure the blank light cube to the instrument.
You do not need to remove the screws.
6. Screw the light cube tool in to the threaded hole in the blank light cube, then lift the blank light
cube up and out of the microscope. Store the blank light cube and the light cube tool in the
accessories box.
Note: Store the shipping restraints and the light cube tool for future use in the accessories box
provided with your system. Always re-secure the X-Y stage with the stage lock pin and re-install the
light cube restraint before moving the instrument.
IMPORTANT! Before changing light channels, ALWAYS verify the light cube restraint has been
removed. Attempting to change the light channels while the restraint is in place can seriously damage
the mechanism. This type of damage is not covered by the manufacturer’s warranty.
Install the UV light shield
1. Verify that the EVOS™Condenser Light Shield™is installed on the condenser assembly. The
condenser shield is pre-installed and helps reduce the potential eects of overhead lighting on
your image.
2. Pull the condenser light shield out from the condenser head.
Chapter3Installation
Install the instrument
3
20 EVOS™M5000 Imaging System User Guide