Invitrogen iBright CL1500 Series User manual

For Research Use Only. Not for use in diagnostic procedures.
iBright™Imaging Systems
USER GUIDE
For use with the iBright™CL1500 Imaging System and the iBright™
FL1500 Imaging System
Catalog Numbers A43678, A44114, A44240 (CL1500) and A43679, A44115, A44241 (FL1500)
Publication Number MAN0018592
Revision A.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL,
INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,
INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018592
Revision Date Description
A.0 June 7 2019 New document. Describes installation, operation, and maintenance of the
iBright™Imaging Systems.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept
the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2019 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■Product information ....................................................... 6
Product description ............................................................. 6
Contents ....................................................................... 6
■Start, sign on, and configure the instrument ............................ 7
Create an instrument profile ...................................................... 7
Create an instrument account and link to Connect using a cloud account ............... 7
Mobile device ............................................................... 8
PC using Connect account .................................................... 8
PC using instrument Connect app ............................................. 8
Use instrument to link to Connect account ..................................... 8
Link to Connect using an existing instrument account ................................ 9
If the unit is off .................................................................. 9
If the unit is on and in sleep mode ................................................. 9
If the unit is on and in active mode ................................................ 10
■Operate the iBright™imager ............................................ 11
Choose imaging mode and start image capture .................................... 11
Chemi Blots ................................................................... 12
Image using Chemi Blots ................................................... 12
Fluorescent Blots (FL1500 model only) ........................................... 13
Image using Fluorescent Blot ............................................... 13
Nucleic Acid Gels .............................................................. 14
Image using Nucleic Acid Gel ................................................ 14
Protein Gels ................................................................... 15
Image using Protein Gels ................................................... 15
Universal Mode ................................................................ 16
Options available in modes ...................................................... 18
Image Gallery ................................................................. 21
View images .............................................................. 21
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■Export and analyze images .............................................. 22
Export images ................................................................. 22
Export new images ........................................................ 22
Export gallery images ...................................................... 22
Select an Export file type ................................................... 23
Analyze images ................................................................ 24
Analyze new images ....................................................... 24
Analyze gallery images ..................................................... 25
■Maintain the instrument ................................................. 27
Settings ....................................................................... 27
Manage accounts .......................................................... 29
Run instrument diagnostics ..................................................... 29
Clean the instrument ........................................................... 30
Replace the instrument fuses .................................................... 30
Materials required ......................................................... 30
Replace the fuses .......................................................... 30
Move the instrument ........................................................... 31
Prepare for shipping ....................................................... 31
■Instrument specifications ............................................... 33
Instrument dimensions and specifications ......................................... 33
Electrical requirements ......................................................... 33
Environmental requirements .................................................... 33
Network requirements .......................................................... 34
Instrument and computer connections ............................................ 35
■Safety ...................................................................... 37
Symbols on this instrument ..................................................... 37
Safety alerts on this instrument ............................................. 38
Location of safety labels on the instrument ................................... 39
Safety information for instruments not manufactured by Thermo Fisher Scientific ..... 39
Instrument safety .............................................................. 39
General .................................................................. 39
Physical injury ............................................................ 39
LED (Light-Emitting Diode) .................................................. 40
Electrical safety ........................................................... 40
Cleaning and decontamination ............................................... 40
Instrument component and accessory disposal ................................ 41
Contents
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Safety and electromagnetic compatibility (EMC) standards .......................... 42
Safety compliance ......................................................... 42
EMC ...................................................................... 42
Environmental design ...................................................... 43
Chemical safety ................................................................ 44
Biological hazard safety ......................................................... 45
■Documentation and support ............................................. 46
Related documentation ......................................................... 46
Customer and technical support ................................................. 46
Limited product warranty ....................................................... 46
Contents
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Product information
IMPORTANT! Before using this product, read and understand the information in the
“Safety” appendix in this document.
Product description
The iBright™ CL1500 Imaging System allows users to image chemiluminescent
Western blots, DNA and RNA gels stained with uorescent nucleic acid stains, and
visible protein gels. The iBright™ FL1500 Imaging System adds the capability of
imaging uorescent Western blots using multiple dyes.
The iBright™ imagers use a simple design and intuitive workows to deliver high-
resolution images. They can be run directly from the touchscreen to start and create
images, while also providing on-board software for image analysis. To further analyze
data, the instrument can be integrated with the iBright™ Image Analysis Software
available for desktop and Connect.
Contents
Table 1 Included imager system parts
Component Quantity
Power cord (region specific) 1
Quick reference card 1
White transilluminator screen 1
iBright™Imaging System Sample Blot 1
Sample stage/turntable 1
Table 2 Available accessories
Component Part number
White transilluminator screen A33828
Sample stage A33829
High-Power USB Wi-Fi Module A26774
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Start, sign on, and configure the
instrument
Create an instrument profile
1. Touch to sign in to your instrument user account.
2. Touch Get started.
3. Touch Create a Prole.
4. Enter a Screen name.
5. Enter and conrm a four-digit pin.
6. Touch Create prole.
7. (Optional) If you want to link your instrument to your Connect account, see “Use
instrument to link to Connect account“ on page 8 or “Link to Connect using an
existing instrument account“ on page 9.
Create an instrument account and link to Connect using a cloud
account
1. Touch to sign in to your instrument user account.
2. Touch Get Started.
3. Click on Connect.
4. Link your account using one of these methods:
•Mobile device — Connect using a QR generated by the instrument and
scanned by the instrument Connect mobile app.
•PC — Connect using a link code that is entered into the instrument Connect
app or your online Connect account.
•Instrument — Enter your Connect account information directly on the
instrument.
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1. Touch Mobile Device.
2. Launch instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on QR code.
6. Scan QR code on the instrument.
Your Connect account should now be linked to your instrument account.
1. Touch PC.
2. Login to your Connect account online.
3. Click on
.
4. Click on .
5. Select iBright from the drop-down menu.
6. Enter the linking code and click on Send.
Your Connect account should now be linked to your instrument account.
1. Touch PC.
2. Launch the instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on Linking code.
6. Enter the linking code and click Send.
Your Connect account should now be linked to your instrument account.
1. Click on Instrument.
2. Enter your Connect username and password.
3. Click Link account.
Your Connect account should now be linked to your instrument account.
Mobile device
PC using Connect
account
PC using
instrument
Connect app
Use instrument to
link to Connect
account
Start, sign on, and configure the instrument
Create an instrument account and link to Connect using a cloud account
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Link to Connect using an existing instrument account
Linking to Connect after account creation can only be done during the export process.
1. Click on .
2. Select an image.
3. Click on Actions.
4. Click on Export.
5. Click on Choose destination.
6. Click on Cloud.
7. Click on Sign in.
8. Connect using one of the methods outlined in “Create an instrument account and
link to Connect using a cloud account“ on page 7.
If the unit is off
1. Ensure the unit is plugged in and turn on using the switch on the back of
instrument.
The instrument will go directly to the sign-in page.
2. Enter a Screen name.
3. Enter and conrm a 4-digit pin.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. The guest gallery is not private. Any logged in user can view and
modify images residing in the guest gallery.
4. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch and place sample in the center of the transilluminator glass.
5. Touch to close drawer (do not physically push on the drawer). Instrument
adjusts and displays a live-view sample image in the viewport.
If the unit is on and in sleep mode
1. Touch the screen to wake.
2. Touch to sign in to use or set up a user prole. See “Create an instrument
prole“ on page 7 for instructions on how to create a user prole.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. The guest gallery is not private. Any logged-in user or other guest
user can view and modify images residing in the guest gallery.
Start, sign on, and configure the instrument
Link to Connect using an existing instrument account
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3. Enter a Screen name.
4. Enter and conrm a 4-digit pin.
5. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch Open Drawer, and place sample in the center of the transilluminator
glass.
6. Touch to close drawer (do not physically push the drawer). Instrument
adjusts and displays a live-view sample image in the viewport.
If the unit is on and in active mode
1. If not already signed in, touch to sign in or set up a new user prole. See
“Create an instrument prole“ on page 7 for instructions on how to create a user
prole.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. The guest gallery is not private. Any logged-in user can view and
modify images residing in the guest gallery.
2. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch and place sample in the center of the turntable glass.
3. Touch to close drawer (do not physically push the drawer). Instrument
adjusts and displays a live-view sample image in the viewport.
Start, sign on, and configure the instrument
If the unit is on and in active mode
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Operate the iBright™imager
Choose imaging mode and start image capture
On the Welcome screen:
1. Use the drop-down menu to select the desired mode.
•Chemi Blots to image chemiluminescent substrates.
•Fluorescent Blots to image uorescent substrates.
•Nucleic Acid Gels to image DNA and RNA gels.
•Protein Gels to image visible, stained protein gels.
•Universal to image samples containing multiple readouts, such as
chemiluminescence, uorescence and/or colorimetric stains. Image display is
similar to Fluorescent Blots and allows you to assign false color to any
sample.
2. Touch to open drawer.
3. Place sample in the center of the viewing area.
4. Touch to close drawer.
Instrument automatically aligns, zooms, focuses, and acquires a live-view sample
image.
Note: If any manual adjustments are required for zoom or focus, touch More
options4Camera and choose the appropriate menu item.
• To image in Chemi Blots mode, proceed to “Image using Chemi Blots“ on
page 12.
• To image in Fluorescent Blots mode, proceed to “Fluorescent Blots (FL1500 model
only)“ on page 13.
• To image in Nucleic Acid Gels mode, proceed to “Image using Nucleic Acid
Gel“ on page 14.
• To image in Protein Gels mode, proceed to “Image using Protein Gels“ on
page 15.
• To image in Universal Mode, proceed to “Universal Mode“ on page 16.
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Chemi Blots
Mode to image chemiluminescent Western blots.
1. Touch Smart Exposure.
The imager acquires a series of short exposures, then renders a preview image
and recommends an exposure time. This is not a real image.
2. Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specic region of the sample.
3. (Optional) Adjust the exposure time using the following methods:
• Touch or .
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
The image preview updates in real-time.
4. Touch Enter (for a custom manual input).
5. Touch Capture to acquire an image.
The captured image is displayed and automatically saves to the gallery.
6. If an acceptable image, touch Export, Gallery, or Analyze. If an unacceptable
image, touch More options to optimize the image or touch Trash to remove the
image, then return to step 2 to adjust exposure conditions.
Image using
Chemi Blots
Operate the iBright™imager
Chemi Blots
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Fluorescent Blots (FL1500 model only)
Mode to image uorescent Western blots using a single channel or up to 4 channels
using excitation wavelengths (FWHM) of approximately 455-485 nm, 515-545 nm,
610-635 nm, 610-660 nm, and 750-768 nm; approximate emission wavelengths
(FWHM) 510-555 nm, 565-615 nm, 675-720 nm, 710-730 nm and 800-850 nm.
1. Touch Choose a dye to select from one to four dyes from the pre-populated list.
a. To edit an existing dye, touch the dye name buon. Touch Dye and change
the assigned dye to any other pre-populated dye in the uorescent dye list.
Dyes in the pre-populated dye list are categorized by most recently used,
name, and emission color.
b. Touch False Color and change the false color assigned to that dye.
Note: Each dye is assigned a false color to be used in the composite overlay.
c. To remove this assigned dye from the channel, touch Remove Dye at the
boom left hand corner of the screen. Touch Conrm.
2. Touch Done.
3. Touch Smart Exposure. Alternatively, for manual exposure, touch More
options4Manual Exposure. For manual exposures, click on the dye to add a
time for each dye selected.
The imager will acquire a series of short exposures for each dye type selected. It
will then render preview images and a recommended exposure time for each
channel. These are not real images.
4. Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specic region of the sample. You can select dierent, same,
or no region of interest for each channel.
5. To adjust or set a manual exposure time for any of the channels you are
acquiring, select the channel (dye) that you want to change:
• Touch or .
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
The image preview updates in real-time.
6. Touch Capture to acquire an image.
The captured image is displayed and automatically saves to the gallery.
7. If an acceptable image, touch Export, Gallery, or Analyze. If an unacceptable
image, touch More options to optimize the image or touch Trash to remove the
image, then return to step 2 to adjust exposure conditions.
Image using
Fluorescent Blot
Operate the iBright™imager
Fluorescent Blots (FL1500 model only)
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Nucleic Acid Gels
Mode to image DNA and RNA gels stained with uorescent nucleic acid stains such
as Ethidium Bromide, SYBR™ Safe, SYBR™ Green (I or II), SYBR™ Gold and similar
products.
1. Touch Smart exposure. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
2. Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specic region of the sample.
3. To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
The image preview will update in real-time.
4. Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
5. If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to step 2 to adjust exposure conditions.
Image using
Nucleic Acid Gel
Operate the iBright™imager
Nucleic Acid Gels
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Protein Gels
•Protein Gels Visible: USE WHITE SCREEN BETWEEN SAMPLE AND
TRANSILLUMINATOR GLASS for samples including Coomassie-stained protein
gels and silver-stained protein gels.
•Protein Gels Fluorescent: PLACE SAMPLE DIRECTLY ON
TRANSILLUMINATOR GLASS for samples including No-Stain™-lableled protein
gels and SYPRO™dye-stained protein gels.
1. Select whether you are imaging a visible sample (Protein Gel Visible) or
uorescent sample (Protein Gel Fluorescent).
2. An auto-zoomed and focused Live View of the image is now displayed in the
viewport.
IMPORTANT! For visual stained protein on nitrocellulose and PVDF membrane,
use Universal Mode4Visible channel without the white screen.
3. Touch Smart Exposure. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
4. Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specic region of the sample. You can select dierent, same,
or no region of interest for each channel.
5. To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
The image preview will update in real-time.
6. Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
7. If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to step 2 to adjust exposure conditions.
Image using
Protein Gels
Operate the iBright™imager
Protein Gels
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Universal Mode
Mode to image samples containing one or more signal types (chemi, uor, and/or
visible).
Table 3 Illumination sources, excitation filters, and emission filters for the iBright™
CL1500 Instrument.
Illumination Excitation Filters Emission Filters
White Epi-LED 455-485 nm
400-700 nm
568-617 nm
ND filter [1] 400-700 nm
Green trans 490-520 nm
[1] Neutral density filter decreases the intensity of the white Epi-LED for visible imaging.
Table 4 Illumination sources, excitation filters, and emission filters for the iBright™
FL1500 Instrument.
Illumination Excitation Filters Emission Filters
White Epi-LED
455-485 nm
400-700 nm
520-555 nm
565-615 nm
675-720 nm
710-730 nm
800-850 nm
515-545 nm
608-632 nm
610-660 nm
nIR Epi-LED 745-765 nm
ND filter 400-700 nm
Green trans 490-520 nm
1. Touch Choose Channel to select from one to four dierent dyes or stains from
the pre-populated list.
a. To edit an existing dye, touch the dye name buon. Touch Dye and change
the assigned dye to any other pre-populated dye in the uorescent dye list.
Dyes in the pre-populated dye list are categorized by most recently used,
name, and emission color.
b. Touch False Color and change the false color assigned to that dye.
Note: Each dye is assigned a false color to be used in the composite overlay.
c. To remove this assigned dye from the channel, touch Remove Dye at the
boom left hand corner of the screen. Touch Conrm.
Operate the iBright™imager
Universal Mode
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Channel refers to the type of signal you want to capture and the imaging
conditions you want to use.
•Chemi: Channel for measuring chemiluminescent signals.
•Fluor [Epi-LED]: Channel for measuring uorescent signals that are excited
using the appropriate Epi-LED/excitation lter combination and the
appropriate emission lter.
•Fluor [Trans]: Channel for measuring uorescent signals that are excited by
the green transilluminator and appropriate emission lter.
•Nucleic acid: Channel for measuring uorescent signals in gel that are
excited by the green transilluminator and the 565-615 emission lter (stained
DNA and RNA).
•Protein: Channel for measuring uorescent and visible signals in gel that are
excited or illuminated by the green transilluminator and the 565-615
emission lter — stained or labeled protein.
•Visible: Channel for measuring visible signals on an opaque media
(nitrocellulose membrane, PVDF membrane, etc.) using the Epi-white LED
with neutral density lter and appropriate emission lter.
•TPN: Total protein normalization channel is for normalizing Western blot
results to total protein load by lane.
2. Touch Smart Exposure. Alternatively, for manual exposure, touch More
options4Manual Exposure. The imager will acquire a series of short exposures
for each dye type selected. It will then render preview images and a
recommended exposure time for each channel. These are not real images.
3. Touch More options4Region of Interest. Region of Interest is for directing
Smart Exposure to a specic region of the sample.
4. To adjust or set a manual exposure time for any of the channels that you are
acquiring, select the channel (dye) that you want to change:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
Note: When recommended exposure time is adjusted, the image preview will
update in real time.
5. Touch Capture to acquire all of the selected channels with the indicated exposure
times. Captured image(s) appears in viewport and automatically saves to the
gallery.
6. If an acceptable image, touch Export, Gallery, or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to steps 3-5 to adjust exposure conditions.
Operate the iBright™imager
Universal Mode
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Options available in modes
Each mode oers More Options on certain workow screens to provide detailed
camera and image adjustment.
Table 5 Camera
Suboption Detail
Resolution/Sensitivity Changes binning setting to increase resolution/decrease
sensitivity or decrease resolution/increase sensitivity.
Zoom/Focus
Changes zoom level to increase or decrease image area.
Optimizes focus for sharpness.
Note: Optical zoom 1X to 2X and digital zoom 2X to 8X.
Sample rotation
Imager mechanically rotates sample ±10° depending on
orientation. Rotation is automatic, but also allows ability to
adjust in More Options4Camera Settings.
Table 6 Routines
Suboption Detail
Multi-exposure Series of 5 preset exposure times producing one image for
each time period.
Signal accumulation
Series of exposures where user defines the first capture
(time interval), the last capture (cumulative capture time)
and the total number of captures.
Note: Images acquired using Signal Accumulation are not
recommended for publication or analysis. This feature is
recommended for determining an optimal exposure time.
Table 7 Image adjust
Suboption Detail
Contrast
Grayscale differentiation between image features. Can use
Auto contrast (high, medium, low) or view raw image with
no contrast. User can manually adjust Gamma, White,
Black using slider bars.
Channels and Layers
Allow user to navigate between individual channels
associated with a common image file; pertains only to
Chemi Blot, Fluorescent Blot, and Universal modes (e.g.,
Channels: membrane, Alexa Fluor™488, Alexa Fluor™555,
Composite). Layers allow toggling on and off analysis tools.
Operate the iBright™imager
Options available in modes
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Suboption Detail
Zoom Increases or decreases digital zoom to select an area of the
image.
Invert Produces a negative or positive (white to black or black to
white) of the displayed image.
Saturation False-colors saturated white pixels (65,536) as red to
differentiate from non-saturated pixels.
Table 8 Other options
Suboption Detail
Region of Interest
Specify an area on the image to determine the optimal
exposure time for that region using the Smart Exposure
tool.
Manual exposure Manually select an exposure time for sample.
Smart exposure
Predicts optimal exposure for minimizing pixel saturation
and maximizing dynamic range for a specific sample.
Renders a preview of how the image will appear at the
recommended exposure time.
Band excision (Nucleic Acid
Gels mode)
Opens drawer and turns on green transilluminator to allow
for safe band excision on nucleic acid gels.
Membrane overlay (Chemi
and Fluorescent Blot
modes)
Allows visible membrane overlay to see any visible
prestained markers, which aid in identifying molecular
weights for unknown samples.
2UP view (Fluorescent Blot
mode)
After a Smart Exposure or Capture, images are displayed
by default in a 2UP view.
• Top image displays individual channels in grayscale.
Switch between individual channels by touching the
channel you want to display and edit. After a dye is
selected, that channel image is displayed in the top
view and becomes editable so the user can increase or
decrease the exposure time dial and see in real time
the effect on the image preview.
• Bottom image displays the color composite with
channels displayed as different false colors overlaid
on a black background. Individual channels can be
toggled on and off in the composite image by touching
at the left of the appropriate channel name.
Operate the iBright™imager
Options available in modes
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Imaging Systems User Guide
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Suboption Detail
1UP view (Fluorescent Blot
mode)
After a Smart Exposure or Capture, images can also be
displayed in a 1UP view.
Image displays the grayscale and false-color composite
images by toggling Gray or Color. User can switch between
Individual channels by touching the dye name you want to
display. Individual channels can be toggled on and off in the
composite image by touching on the left of tehe
appropriate dye name.
Note: Functionality for toggling between individual
channels in grayscale view or toggling on and off individual
channels in the false-color composite view is identical to
the 2UP view.
Auto Enhance
Auto Enhance applies rolling-ball correction to the
composite image of a multichannel image. It can be
toggled on and off and only affects the visible image, not
the raw data.
Operate the iBright™imager
Options available in modes
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