Invitrogen iBright CL1500 Series User manual

For Research Use Only. Not for use in diagnostic procedures.
iBright™Imaging Systems
USER GUIDE
For use with the iBright™CL1500 Imaging System and the iBright™FL1500
Imaging System
Catalog Numbers A44114 (CL1500) and A44115 (FL1500)
Publication Number MAN0018592
Revision D.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
Revision history:Pub.No. MAN0018592
Revision Date Description
D.0 31 October 2022
•Updating workflows according to instrument firmware upgrades.
•Adding SmartRange™HDR functionality and analysis of E-gel and colony images in Universal
mode.
C.0 24 August 2020 Adding SAE chapter due to addition of SAE instrument functionality.
B.0 23 September 2019
•Updated the components list (see Table 1 on page 6).
•Updated the recommended excitation/emission wavelengths to image fluorescent Western blots
(see "Fluorescent Blots (FL1500 model only)" on page 14).
•Updated the emission filters for the iBright™FL1500 Instrument (see Table 4 on page 18).
•Added additional outputs for TPN-normalized data.
•Added additional information for resetting factory defaults, backing up, and restoring settings (see
"Settings" on page 30).
A.0 7 June 2019 New document for iBright™Imaging Systems.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product,
you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2022 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER1Productinformation ................................................. 7
Productdescription ............................................................. 7
Contents ....................................................................... 7
■CHAPTER2Start, sign on, and configure theinstrument ..................... 9
Create an instrumentprofile ...................................................... 9
Create an instrument account and link to Connect using a cloudaccount .............. 9
Mobiledevice ............................................................. 10
PC using Connectaccount ................................................. 10
PC using instrument Connectapp ........................................... 10
Link to Connect using an existing instrumentaccount .............................. 11
If the unit iso ................................................................. 11
If the unit is on and in sleepmode ................................................ 12
If the unit is on and in activemode ............................................... 12
■CHAPTER3Operate the iBright™imager ...................................... 13
Choose imaging mode and start image capture .................................... 13
ChemiBlots ................................................................... 14
Image using ChemiBlots ................................................... 14
Fluorescent Blots (FL1500 modelonly) ............................................ 15
Image using FluorescentBlot ............................................... 15
Nucleic AcidGels .............................................................. 17
Image using Nucleic AcidGel ............................................... 17
ProteinGels ................................................................... 18
Image using ProteinGels ................................................... 18
UniversalMode ................................................................ 19
Options available inmodes ...................................................... 21
Image Gallery .................................................................. 24
Viewimages .............................................................. 24
iBright™Imaging Systems User Guide 3

■CHAPTER4Export and analyzeimages ....................................... 25
Exportimages ................................................................. 25
Export newimages ........................................................ 25
Export galleryimages ...................................................... 25
Select an Export filetype ................................................... 26
Archive/Backup g2i imagefiles .............................................. 27
Analyzeimages ................................................................ 28
Analyze newimages ....................................................... 28
Analyze galleryimages ..................................................... 30
Analyze 96-well E‑Gel™images taken in Universal mode using the E‑Gel™
96application ........................................................... 30
Analyze colony images taken in Universal mode using the Colony
Countapplication ........................................................ 31
■CHAPTER5Maintain theinstrument ........................................... 33
Settings ....................................................................... 33
Manageaccounts .......................................................... 35
Run instrumentdiagnostics ..................................................... 36
Clean theinstrument ........................................................... 36
Replace the instrumentfuses .................................................... 37
Materials required .......................................................... 37
Replace thefuses .......................................................... 37
Move theinstrument ........................................................... 38
Prepare forshipping ....................................................... 38
■CHAPTER6Invitrogen™iBright™SAE Software Solution for 21 CFR
Part 11 Support ................................................................... 39
Security Audit e-Signature (SAE) for the iBright™Imager ............................ 39
Activate your license key ........................................................ 40
Enable SAEmode .............................................................. 40
Disable SAEmode ............................................................. 41
Sign in to the instrument inSAE mode ............................................ 41
Change your password in SAEmode ............................................. 42
SAE user roles ................................................................. 42
SAE privileges for default user roles .............................................. 43
Non-SAE user roles ............................................................ 43
Acquire images in SAEmode .................................................... 44
Contents
4iBright™Imaging Systems User Guide

Use the Gallery in SAEmode .................................................... 44
Filestatus ................................................................ 44
Filter Gallery by filestatus .................................................. 44
Archive/Backup g2i image files in SAE mode ................................. 44
Commitchanges ............................................................... 45
Signdata ..................................................................... 46
Unlockfiles ................................................................... 46
Generate an audit history ....................................................... 47
View the e-Signature history ..................................................... 47
Export imagefiles .............................................................. 47
Generate and export Analysis Report ............................................. 48
■APPENDIXAInstrumentspecifications ........................................ 49
Instrument dimensions andspecifications ......................................... 49
Electrical requirements ......................................................... 49
Environmental requirements ..................................................... 49
Network requirements .......................................................... 50
Instrument and computerconnections ............................................ 51
■APPENDIXBSafety .............................................................. 53
Symbols on thisinstrument ..................................................... 53
Location of safety labels on theinstrument .................................... 55
Safety information for instruments not manufactured by Thermo FisherScientific ...... 55
Instrumentsafety .............................................................. 55
General ................................................................... 55
Physical injury ............................................................. 56
LED (Light-EmittingDiode) .................................................. 56
Electricalsafety ........................................................... 57
Cleaning anddecontamination .............................................. 58
Instrument component and accessorydisposal ................................ 58
Safety and electromagnetic compatibility (EMC) standards .......................... 59
Safetycompliance ......................................................... 59
EMC ..................................................................... 59
Environmentaldesign ...................................................... 60
Chemicalsafety ................................................................ 62
Biological hazardsafety ......................................................... 63
Contents
iBright™Imaging Systems User Guide 5

■APPENDIXCDocumentation and support .................................... 64
Related documentation and references ........................................... 64
Customer and technical support ................................................. 65
Limited product warranty ........................................................ 65
Contents
6iBright™Imaging Systems User Guide

Product information
IMPORTANT! Before using this product, read and understand the information in the “Safety”
appendix in this document.
Product description
The iBright™CL1500 Imaging System allows users to image chemiluminescent Western blots, DNA and
RNA gels stained with fluorescent nucleic acid stains, and visible protein gels. The iBright™FL1500
Imaging System adds the capability of imaging fluorescent Western blots using multiple dyes.
The iBright™imagers use a simple design and intuitive workflows to deliver high-resolution images.
They can be run directly from the touchscreen to start and create images, while also providing on-board
software for image analysis. To further analyze data, the instrument can be integrated with the iBright™
Image Analysis Software available for desktop and Connect.
Contents
Table1Included imager system parts
Component Quantity
iBright™Imaging System 1
Power cord (region specific) 1
Quick Reference Guide 1
Contrast tray 1
iBright™Imaging System Sample Blot 1
Sample stage/turntable 1
Product Information Sheet 1
Safe Imager™Viewing Glasses 1
1
iBright™Imaging Systems User Guide 7

Table2Available accessories
Component Part number
iBright™System Contrast Tray A56597
Sample stage A33829
High-Power USB Wi-Fi Module A26774
iBright™E‑Gel™Tray Adapter
A56600 (11/22)
A56599 (48/96)
Chapter1Product information
Contents
1
8iBright™Imaging Systems User Guide

Start, sign on, and configure the
instrument
Create an instrument profile
1. Touch to sign in to your instrument user account.
2. Touch Get started.
3. Touch Create a Profile.
4. Enter a Screen name.
5. Enter and confirm a four-digit pin.
6. Touch Create profile.
7. (Optional) If you want to link your instrument to your Connect account, see “Link to Connect using
an existing instrument account” on page11.
Create an instrument account and link to Connect
using a cloud account
1. Touch to sign in to your instrument user account.
2. Touch Get Started.
3. Click on Connect.
4. Link your account using one of these methods:
•Mobile device — Connect using a QR generated by the instrument and scanned by the
instrument Connect mobile app.
•PC — Connect using a link code that is entered into the instrument Connect app or your
online Connect account.
•Instrument — Enter your Connect account information directly on the instrument.
2
iBright™Imaging Systems User Guide 9

Mobile device
1. Touch Mobile Device.
2. Launch instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on QR code.
6. Scan QR code on the instrument.
Your Connect account should now be linked to your instrument account.
PC using Connect account
1. Touch PC.
2. Login to your Connect account online.
3. Click on
.
4. Click on .
5. Select iBright from the drop-down menu.
6. Enter the linking code and click on Send.
Your Connect account should now be linked to your instrument account.
PC using instrument Connect app
1. Touch PC.
2. Launch the instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on Linking code.
6. Enter the linking code and click Send.
Your Connect account should now be linked to your instrument account.
Chapter2Start, sign on, and configure the instrument
Create an instrument account and link to Connect using a cloud account
2
10 iBright™Imaging Systems User Guide