Invitrogen Dynal MPC-1 User manual

Magnetic Particle Concentrator
PRINCIPLE
Dynal® MPC™-1 is a tool to separate Dynabeads®
from diverse liquid sample matrices. The Dyna-
beads will be attracted to the magnet adjacent to
the tube wall when the tube is inserted into the
Dynal MPC-1. This enables easy removal of super-
natant while the Dynabeads are left isolated in the
tube. Any laboratory skilled in using conventional
laboratory techniques that follow good laboratory
practices may use Dynal MPC-1 for magnetic sepa-
ration with Dynabeads.
PRODUCT DESCRIPTION
The Dynal MPC-1 is made from disinfectant proof
polyacetate equipped with Invitrogen Dynal rare
earth magnets (Neodymium-Iron-Boron perma-
nent magnets).
The Dynal MPC-1 has been designed to hold one
single tube with variable diameters (up to 30 mm)
and volumes (5-50 ml).
Nominal magnetic properties
Neodymium-Iron-Boron permanent magnets
Br (Remanence):
12,200 Gauss 1,220 mTesia
bHc (Coercive Force):
11,400 Oersteds 410 kA/m
jHc (Intrinsic Coercive Force):
17,000 Oerstedt 1,350 kA/m
BHmax (Maximum Energy Product):
38 x 106 GOe 306 kJ/m3
The high permanent magnetic properties ensure
satisfactory isolation of Dynabeads immunomag-
netic beads.
WARNING
The Health and Safety Officer should
take all necessary steps and full respon-
sibility to ensure that the following pre-
cautions and statements are followed
and adhered to. IN NO EVENT SHALL
INVITROGEN DYNAL BE LIABLE FOR
ANY SPECIAL, INCIDENTAL OR CONSE-
QUENTIAL DAMAGES.
NOTE
The Dynal MPC-1 should not be kept in
close contact with magnetic tapes, cre-
dit cards, computer discs or other mag-
netic storage systems, as these can be
damaged by the strong magnetic field.
Also keep away from pacemakers.
INSTRUCTIONS FOR USE
1. Dynabeads are incubated with the desired tar-
get in a test tube according to the experimental
protocol.
2. For magnetic separation, place the test tube in
the Dynal MPC-1 and ensure that the clamp
holds the tube in place.
3. Allow the test tube to remain in the Dynal MPC-
1 for 1-4 minutes. During this time the Dyna-
beads-target complexes will be attracted to the
wall of the test tube by the magnetic field. The
magnetic separation may, depending on the
experimental conditions, be enhanced by an
extended separation time and an occasional,
gentle tilting of the Dynal MPC-1 while the test
tube is held in position.
4. Remove the supernatant, either by pipetting or
decanting the test tube while it remains in the
Dynal MPC-1. The Dynabeads-target complexes
are kept on the wall of the test tube by the
magnetic field.
Positive isolation
5. After discarding the supernatant (step 4 above),
remove the test tube from the Dynal MPC-1.
Add wash solution along the wall of the test
tube where the Dynabeads-target complexes
are attached.
6. Place the test tube in the Dynal MPC-1 for
1-4 minutes and again remove the supernatant.
Repeat the washing step according to the speci-
fic experimental protocol.
7. Discard the final wash solution and resuspend
the Dynabeads-target complexes in an appro-
priate medium, again according to the specific
experimental protocol. The Dynabeads-target
complexes can be concentrated as a pellet in
the bottom of the test tube by slowly sliding the
tube out of the Dynal MPC-1.
Negative isolation and depletion
Magnetic separation can also be used to remove
unwanted target from a heterogenous suspension.
Using this approach, the bead-bound target is
discarded and the untouched target of interest is
recovered with a pipette (the supernatant in step 4
above).
NOTE
Immunomagnetic isolation is, as all
immunological techniques, critically de-
pendent on the specificity and the avidi-
ty of the antibodies used with the
immunomagnetic beads. The Dynal
MPC-1 only guarantees a satisfactory
isolation of Dynabeads, not the isolation
of a certain antigen or cell.
DISINFECTION
The following materials have undergone testing
regimes for cleaning purposes. Spray and/or wipe
the Dynal MPC-1 unit with one of the following
cleaning agents:
• 70% isopropyl alcohol
• 1% sodium hypochlorite solution (Bleach)
• 0.1 M HCl solution
Other disinfectants have not been tested and may
not be suitable. Avoid prolonged exposure to wa-
ter or aqueous solutions.
NOTE
Do not autoclave the Dynal MPC-1.
GENERAL INFORMATION
Invitrogen Dynal AS complies with the Quality
System Standards ISO 9001:2000 and ISO
13485:2003.
STORAGE AND STABILITY
Your Dynal MPC-1 contains sintered rare-earth
Neodymium Iron Boron permanent magnets.
Magnetic strength will not diminish significantly
during the lifetime of the product. Loss of magne-
tic strength will occur only if the magnets are
exposed to temperatures greater than 90°C. Do
not leave Dynal MPC-1 exposed to UV light, as the
surface material may become brittle.
Technical Support
Please contact Invitrogen Dynal AS for further
technical support (see contact details).
WARNINGS AND LIMITATIONS
Handle the Dynal MPC-1 with care. The Invitrogen
Dynal magnets are brittle and can be damaged
with improper handling.
Dynal® MPC™-1
Cat. no. 120.01D
Rev. no. 006

Trademarks and Patents
Dynal®, Dynabeads® and Dynal MPC™ are either
registered trademarks or trademarks of Invitrogen
Dynal AS, Oslo, Norway. Any registration or trade-
mark symbols used herein denote the registration
status of trademarks in the United States.
Trade mar ks may or may n ot be r egi ste red i n oth er
countries.
Intellectual Property Disclaimer
Invitrogen Dynal will not be responsible for viola-
tions or patent infringements that may occur with
the use of our products.
Limited Use Label License
No. 358: Research Use Only
The purchase of this product conveys to the pur-
chaser the limited, non-transferable right to use
the purchased amount of the product only to per-
form internal research for the sole benefit of the
purchaser. No right to resell this product or any of
its components is conveyed expressly, by implic-
ation, or by estoppel. This product is for internal
research purposes only and is not for use in com-
mercial applications of any kind, including, without
limitation, quality control and commercial services
such as reporting the results of purchaser’s activit-
ies for a fee or other form of consideration. For
information on obtaining additional rights, please
contact [email protected] or Out Licensing,
Life Technologies, 5791 Van Allen Way, Carlsbad,
California 92008.
Warranty
The products are warranted to the original pur-
chaser only to conform to the quantity and contents
stated on the vial and outer labels for the duration
of the stated shelf life. Invitrogen Dynal's oblig-
ation and the purchaser's exclusive remedy under
this warranty is limited either to replacement, at
Invitrogen Dynal's expense, of any products which
shall be defective in manufacture, and which shall
be returned to Invitrogen Dynal, transportation
prepaid, or at Invitrogen Dynal's option, refund of
the purchase price.
Claims for merchandise damaged in transit must
be submitted to the carrier.
This warranty shall not apply to any products
which shall have been altered outside Invitrogen
Dynal, nor shall it apply to any products which
have been subjected to misuse or mishandling.
ALL OTHER WARRANTIES, EXPRESSED, IMPLIED
OR STATUTORY, ARE HEREBY SPECIFICALLY EX-
CLUDED, INCLUDING BUT NOT LIMITED TO
WARRANTIES OF MERCHANTABILITY OR FIT-
NESS FOR A PARTICULAR PURPOSE. Invitrogen
Dynal's maximum liability is limited in all events to
the price of the products sold by Invitrogen Dynal.
IN NO EVENT SHALL INVITROGEN DYNAL BE
LIABLE FOR ANY SPECIAL, INCIDENTAL OR CON-
SEQUENTIAL DAMAGES. Some states do not allow
limits on warranties, or on remedies for breach in
certain transactions. In such states, the limits set
forth above may not apply.
Invitrogen Dynal is a part of the Invitrogen Group.
Contact details for your local Invitrogen sales
office/technical support can be found at
http://www.invitrogen.com/contact
© Copyright 2007,2010 Invitrogen Dynal AS, Oslo,
Norway. All rights reserved.
Revised: 12.2010
Printed: 04.2007