IT-IS Life Science MyGo Pro User manual

PERFORMANCE MADE EASY
USER GUIDE
MyGo

WELCOME
Thank you for choosing the MyGo Pro.
The MyGo Pro provides unmatched performance






Performance made easy...

CONTENT

to start running your MyGo Pro. It describes everything


The contents of the guide include...
SETUP
Learn how to install your
MyGo Pro - from seng it
up on your workbench, to
conguring the instrument
and installing the soware.
TIPS
Learn some useful ps when
using your instrument.
EXPERIMENT
Learn the basics of seng
up an experiment on your
MyGo Pro.
ANALYSIS
Learn how to easily analyse
your results.
EXPORT
Learn how to export your data
for use with other soware.

Learn how to look aer your
MyGo Pro.
TROUBLESHOOTING
If you ever have a problem
with your instrument, learn
how to troubleshoot it here.


2. MYGO PRO USER GUIDE
3.

5. USB DRIVE
Please keep the ight case and outer box the MyGo Pro came
in, in case you need to protect your MyGo Pro during storage or
shipment in future.
Lid
USB Port
SETUP
INSTALLING YOUR MYGO PRO
You should have the following items in the MyGo Pro ight case:

LID
The lid ensures that tubes are seated correctly in the wells,
provides opcal isolaon, includes a heated lid to reduce
condensaon, and prevents dust falling into empty wells.
DISPLAY LEDs
The MyGo Pro uses display LEDs to help you understand what
the instrument is doing. Here is a summary of these display LEDs.
Blue 
Green Idle
Red Lid open - Please close the lid
Yellow Tubes Loaded
Blue 
Pink 
Red 
Cyan Experiment complete
Red-Flashing Fault detected
USB PORT
Insert the USB drive here to run an experiment from the
USB drive.

MYGO PRO CONNECTIONS


This is for connecng your
MyGo Pro to your LAN or
computer.
This is to connect your MyGo
Pro to the provided power
supply unit.
ETHERNET PORT
POWER
Ethernet Port
Power



directly to your computer. Once your MyGo Pro


POWERING ON YOUR MYGO PRO




a lid is closed or red if not. Your MyGo Pro is ready to

If you are not using an earthed supply then you must

CONNECTING YOUR MYGO PRO




MYGO PRO HEATED LID

not touch it. 
105º


conserve energy.
Heated Lid
Do not touch





mygopcr.com.
WINDOWS
Double-click on the
Windows installer and follow
the on-screen instrucons
to install your MyGo Pro
soware on Windows.

Double-click on the
disk image and follow
the on-screen instrucons
to install your MyGo Pro
soware on your Mac PC.
INSTALLING YOUR
MYGO PRO SOFTWARE



to your MyGo Pro Instrument.
Please select Conguraon
CONFIGURING YOUR
MYGO PRO

To add your new MyGo Pro
select the Add b uon. You
will now be presented with
a list of available instruments.
Please select MyGo Pro

Double-click on the
instrument you wish to
connect to, or press Select
with the instrument selected.
The instrument will now
be added to the list of
Registered Instruments
for use in the soware.

TIPS
Here are some great ps to keep in mind whilst running your
MyGo instrument.
1. LID GETS WARM
2. DO NOT LEAVE THE LID OPEN
3. SPIN YOUR TUBES
4. REMOVE ALL BUBBLES



LID GETS WARM
The MyGo Pro lid can get warm during operaon, this is
completely normal.
SPIN YOUR TUBES
This will ensure all well contents are at the boom of the
wells and will improve performance.
DO NOT LEAVE THE LID OPEN
If the lid is open dust may fall into the wells and aect the
performance of your instrument.

REMOVE ALL BUBBLES
Bubbles can cause opcal artefacts as shown in the graph
below. Ensure that no bubbles are present in reacon volumes.

Please keep your work space clean including all lab equipment
like surfaces, pipees, and tube racks. This will keep the
instrument clean and help maintain good results.

We recommend a roune cleaning of your instrument. To do so
follow the cleaning guide in the Maintenance section.

Please disable all power saving sengs e.g. sleep and hibernate.
Intensity (RFU)
Cycle

CREATE A NEW EXPERIMENT
EXPERIMENT








Click New to create
a new experiment.

EXPERIMENT SUMMARY
Once you have created a new experiment the
Experiment Summary w ill be displayed as shown below:
By default the experiment
name will be “New
Experiment” with a date
and me stamp, which
can be edited.
The summary will give
you informaon about the
instrument you are running,
the sengs chosen, and any
useful notes you choose to
add about your experiment.

The Files of Type drop down
menu gives you the opon to
open an experiment normally,
or as a template that can be
used for a new run.
OPEN AN EXPERIMENT
Open you can open an experiment as

FILES OF TYPE

EXPERIMENT FILE
An experiment le will contain
all the data and analyses, but
can not be re-run.
To open as an Experiment
File s elect the rst opon
shown on the opposite page.
EXPERIMENT FILE
AS TEMPLATE
Opening an experiment le
as a template allows you to
use the same prole, samples,
sengs, and analyses to
re-run a previous experiment,
generang new data. When an
experiment le is opened as
a template, it has the same:
1. Temperature profile.
2. Optical settings.
3. Samples and targets.
4. Analyses, including sengs.
If you wish to open an
Experiment File as Template
s elect the second opon.
USB RUN
To open a USB run navigate to the USB locaon using the Open
dialog window shown to the le. Select Experiment File in the
Files of Type drop down menu, and then double click on the
USB run.

SAVING AN EXPERIMENT


saved by you. The experiment must be saved before the


SAVING
When starng a run you will
be prompted with the window
displayed below.
Make sure the rst checkbox is selected and your
experiments will be automacally saved. To prevent
the soware from showing this dialog again, select the
second checkbox. You can change these sengs later
by clicking Conguraon and selecng General Cong.
To automacally save
your data when the run
has nished, rstly click
Browse and choose the
locaon to save your run to.