KD Scientific KDS LEGATO 100 User manual

KDS Legato 100 Series
USER’S MANUAL
Publication 5617-006 Rev 2.0
www.kdscientific.com
KDS LEGATO 100 SERIES

5617-006DC Rev 1.0
Declaration of Conformity
Manufacturer: KD Scientific, Inc.
84 October Hill Road
Holliston, Massachusetts 01746-1388, U.S.A.
Phone: [phone]
We herewith declare that the following product:
Product Name: KDS Legato 100 Series Syringe Pump Series
Model No.: KDS Legato 100 Series - Catalog # 7881xx
KDS Legato 950 series- Catalog # 7889xx
To which this declaration relates, is in conformity with the applicable EC Directives, harmonized standards, and
other normative requirements:
Application of Council Directive(s):
2006/95/EC Low Voltage Directive
2004/108/EC Electromagnetic Compatibility Directive
2011/65/EU RoHS Directive
2012/19/EU WEEE Directive
Standard(s) to which conformity is declared:
Safety: IEC 61010-1:2010 (3rd Edition)
UL 61010-1:2012 (3rd Edition)
CSA C22.2# 61010-1:2012 (3rd Edition)
Emissions/Immunity: IEC 61326-1:2013
FCC 47CFR 15B:2014, Class B
EMC and Safety compliance were evaluated by Intertek/ETL Semko
Reference test reports file numbers: 100602821 Box-001, -001a
100179785 Box –001, -003
100179785 Box –005a, -005b
I, the undersigned, hereby declare that the equipment specified above conforms to the above Directive(s) and Standard(s).
Date: July 10, 2015
Signed:
Harvard Bioscience Global Director
Manufacturing and Process Engineering

2
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
SUBJECT PAGE NO.
Table of Contents
General Information ................................................................................................. 6
Safety Information............................................................................................................................ 6
Warranty and Repair Information.................................................................................................. 7
Manual Description ................................................................................................................ 7
Warranty.................................................................................................................................. 7
Repair Facilities and Parts ...................................................................................................... 7
Serial Numbers ....................................................................................................................... 7
Calibrations ............................................................................................................................. 7
Specifications ................................................................................................................................... 8
Product Overview ...................................................................................................10
Pump Models .................................................................................................................................11
Legato 100 Series Touchscreen Button Reference.....................................................................12
Selecting an Item from a Menu List ..............................................................................................13
Messages .......................................................................................................................................13
Entering Values ..............................................................................................................................13
Operating Instructions ...................................................................................................................14
Power Connections........................................................................................................................14
Syringe Loading.............................................................................................................................15
Configuring the KDS Legato 100 Series................................................................16
Selection Mode..............................................................................................................................16
Syringe Selection ...........................................................................................................................17
Flow Rate Selection .......................................................................................................................18
Target Volume/Time Selection .....................................................................................................19
Previewing the Configuration .......................................................................................................20

3
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
Configuring the Pump’s General Parameters..............................................................................20
Setting the Audible Alarms...................................................................................................21
For Near End of Run Selection ............................................................................................22
Locking the Touchscreen.......................................................................................................23
Setting the Force Limits..........................................................................................................24
Setting the Display Brightness ..............................................................................................25
Setting the Pump Address.....................................................................................................26
Setting the RS 232 Baud Rate .............................................................................................26
Resume Run on Power Loss ..................................................................................................27
Setting and Viewing Pump Information...............................................................................27
Operating the KDS Legato 100 Series..................................................................28
Running the Pump ..........................................................................................................................29
Custom Program Workflow ..........................................................................................................30
Step-by-Step Operating Example ........................................................................................31
Configuring Custom Programs......................................................................................................32
Configuring New Custom Program..............................................................................................32
Setting a Delay...............................................................................................................................34
Setting a Delay for Program Operation..............................................................................34
Creating a Custom New Program................................................................................................35
To Create a New User-Defined Program ...........................................................................35
Modifying an Existing/User-Defined Program............................................................................37
Changing the Order of Steps in a Program........................................................................37
Deleting a Step from a Program..........................................................................................37
Defining Program Step Parameters ..............................................................................................37
Pump Operation Profiles ...............................................................................................................38
Constant Rate Profile.............................................................................................................39
Ramp Profile...........................................................................................................................40
SUBJECT PAGE NO.

4
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
Delay......................................................................................................................................41
Repeat Step ...........................................................................................................................41
Stop ........................................................................................................................................42
I/O Event Triggers ................................................................................................................43
I/O (OUT) Trigger (OUT)........................................................................................................44
I/O (IN) Event ............................................................................................................................45
Managing Legato 100 Series Programs.....................................................................................46
Saving a Copy of a Program...............................................................................................46
Renaming a Program ............................................................................................................46
Appending a Program..........................................................................................................47
Deleting a Program...............................................................................................................47
Program Export and Import ..........................................................................................................48
Transfer a Program between Pumps....................................................................................48
Exporting a Program to an External Computer ..................................................................49
Importing a Program from an External Computer..............................................................49
External Pump Control...................................................................................................................50
Setting Up HyperTerminal....................................................................................................50
Pump Chain Commands................................................................................................................51
System Commands................................................................................................................52
Run Commands .....................................................................................................................54
Rate Commands ....................................................................................................................54
Volume Commands...............................................................................................................55
Time Commands....................................................................................................................56
Digital I/O Commands.........................................................................................................57
Internal Commands...............................................................................................................57
Error Codes ....................................................................................................................................58
SUBJECT PAGE NO.

5
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
Appendices ..............................................................................................................60
Appendix A: Syringe Volume/Diameter Reference Table.........................................................60
Appendix B: Min/Max Flow Rates..............................................................................................61
Appendix C: External Connections..............................................................................................63
User I/O Connector Specifications.....................................................................................63
Utilizing USB Interface..........................................................................................................64
RS-485 ...................................................................................................................................66
Appendix D: Maintenance ...........................................................................................................67
Maintenance.....................................................................................................................67
Upgrading Legato 100 Series Software........................................................................68
Troubleshooting................................................................................................................69
Appendix E: Ordering Information ..............................................................................................70
SUBJECT PAGE NO.

6
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
General Information
SAFETY INFORMATION
Please read the following safety precautions to ensure proper use of your syringe pump. If the equipment is used
in a manner not specified, the protection provided by the equipment may be impaired.
To Prevent Hazard or Injury:
USE PROPER POWER SUPPLY
The pump is supplied with an approved power supply and line cord. To maintain the safety integrity of the device,
use only the following power supplies:
Cui Inc. Globtek Inc.
Model: 3A – 242DB24 Model: GT–41062–T3
Output: 24V – 1.0A Output: 24V – 0.75A
Input: 100–240V~50–60 Hz 0.8A Input: 100–240V~50–60 Hz 0.6A
USE PROPER LINE CORD
Use only the specified line cord for this product and make sure line cord is certified for country of use. The
operating voltage range for the KDS Legato 100 Series is 12-30 VDC. The universal power supply operating voltage
range is 100-240 VAC, 50-60 Hz.
GROUND THE PRODUCT
This product is grounded through the grounding conductor of the power cord. To avoid electric shock, the
grounding conductor must be connected to earth ground. Before making any connections to the input or output
terminals of the product, ensure that the product is properly grounded.
MAKE PROPER CONNECTIONS
Make sure all connections are made properly and securely. Any signal wire connections to the unit must be no longer than 3
meters.
OBSERVE ALL TERMINAL RATINGS
Review the operating manual to learn the ratings on all connections.
AVOID EXPOSED CIRCUITRY
Do not touch any electronic circuitry inside of the product.
AVOID PINCH HAZARD
A pinch hazard may exist between the pusher block and end blocks. Avoid placing fingers between these points
while the pump is running.
DO NOT OPERATE WITH SUSPECTED FAILURES
If damage is suspected on or to the product do not operate the product. Contact qualified service personnel to perform
inspection.
ORIENT THE EQUIPMENT PROPERLY
Do not orient the equipment so that it is difficult to operate the disconnection device.
PLACE PRODUCT IN PROPER ENVIRONMENT
Review the operating manual for guidelines for proper operating environments.
OBSERVE ALL WARNING LABELS ON PRODUCT
Read all labels on product to ensure proper usage.
Protective Ground
Terminal
CAUTION
Refer to Manual
!

7
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
WARRANTY AND REPAIR INFORMATION
Manual Description
This manual is designed to provide all operational and program information required to operate and maintain the
KDS Legato 100 Series pumps. The functions and features are described in the Technical Specifications section.
Warranty
KD Scientific warranties this instrument for a period of one year from date of purchase. At its option, KD Scientific will
repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty does not extend
to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends only to
the original consumer purchaser.
IN NO EVENT SHALL KD SCIENTIFIC BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some
states do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or
exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS
FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied
warranty, so the above limitation may not apply to you.
If a defect arises within the warranty period, promptly contact KD Scientific, 84 October Hill Road, Holliston,
Massachusetts 01746 at 1–508-429-6809. Email address is info@kdscientific.com. Goods will not be accepted
for return unless an RMA (returned materials authorization) number has been issued by our customer service
department. The customer is responsible for shipping charges for non-warranty repairs. Please allow a reasonable
period of time for completion of repairs or replacement. If the unit is replaced, the replacement unit is covered only
for the remainder of the original warranty period dating from the purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary from state to state.
Repair Facilities and Parts
KD Scientific stocks replacement and repair parts. When ordering, please describe parts as completely as possible,
preferably using a part number obtained from our Customer Service department. If practical, enclose a sample part or
sketch. We offer a complete reconditioning service.
Serial Numbers
All inquiries concerning our product should refer to the serial number of the unit, located on the rear panel.
Calibrations
All electrical apparatus are calibrated at rated voltage and frequency. While the flow and volume will stay
calibrated, the peak pressure may vary. KD Scientific recommends an annual calibration of the pump.
!CAUTION: For research use only. Not for clinical use on patients.
!CAUTION: REFER TO SAFETY INFORMATION AND SETTING UP THE KDS LEGATO
100 SERIES BEFORE PLUGGING IN THE PUMP.

8
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
Specifications Legato 100, 101, 110, 111 Legato 180
Accuracy ± 0.5% ± 0.35%
Reproducibility ± 0.05% ± 0.05%
Syringes (Min./Max.) 0.5 µl / 60 ml (10 ml dual) 0.5 µl / 10 ml
Flow Rate:
Minimum (0.5 µl syringe) 1.26000 pl/min 0.54000 pl/min
Maximum (10 ml syringe) 26.0170 ml/min 11.7027 ml/min
Maximum (60 ml syringe) 88.4040 ml/min N/A
Display 4.3” WQVGA TFT Color Display
with Touch Screen
4.3” WQVGA TFT Color Display
with Touch Screen
Non-Volatile Memory Stores all settings Stores all settings
Connectors:
RS-485 IEEE-1394, 6 pos IEEE-1394, 6 pos
USB Type B Type B
I/O & TTL 15 pin D-Sub Connector 15 pin D-Sub Connector
Footswitch mini phone jack mini phone jack
Linear Force (Max) 13.6 kg (30 lbs) @ 100% Force Selection 13.6 kg (30 lbs) @ 100% Force Selection
Drive Motor 0.9° Stepper Motor 0.9° Stepper Motor
Motor Drive Control Microprocessor with 1/16 microstepping Microprocessor with 1/16 microstepping
Number of Microsteps
per one rev. of Lead Screw
15,360 20,480
Step Resolution 0.069 µm/µstep 0.031 µm/µstep
Step Rate:
Minimum 27.5 sec/µstep 27.5 sec/µstep
Maximum 26 µsec/µstep 26 µsec/µstep
Pusher Travel Rate:
Minimum 0.15 µm/min 0.02 µm/min
Maximum 159.00 mm/min 71.55 mm/min
Input Power 12-30 VDC 12-30 VDC
Input Power Connection 2.5mm ID x 5.5mm OD male plug 2.5mm ID x 5.5mm OD male plug
Power Supply 100-240 VAC, 50-60 Hz, 8 Watts Universal
Power Supply, Use Only a KD Scientific
Approved Power Supply and Line Cord
100-240 VAC, 50-60 Hz, 8 Watts Universal
Power Supply, Use Only a KD Scientific
Approved Power Supply and Line Cord
Dimensions 22.6 x 19.05 x 15 cm (9 x 7.5 x 5 in) 22.6 x 19.05 x 15 cm (9 x 7.5 x 5 in)
Weight 2.66 kg (5.9 lbs) 2.66 kg (5.9 lbs)

9
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
Specifications Legato 100, 101, 110, 111 Legato 180
Atmospheric Specifications:
Operating Temperature 4°C to 40°C (40°F to 104°F) 4°C to 40°C (40°F to 104°F)
Storage Temperature -10°C to 70°C (14°F to 158°F) -10°C to 70°C (14°F to 158°F)
Operating Humidity See Chart Below See Chart Below
Storage Humidity 20% to 80% RH, non condensing 20% to 80% RH, non condensing
Method of Operation Continuous Continuous
Classification Class I Class I
Pollution Degree 1 1
Installation Category II II
Supplier Name KDS KDS
Supplier Address 84 October Hill Rd., Holliston, MA 01746 84 October Hill Rd., Holliston, MA 01746
Supplier Phone Number [phone] [phone]
Regulatory Certifications CE, ETL, (UL, CSA), CB Scheme, EU RoHS,
WEEE
CE, ETL, (UL, CSA), CB Scheme, EU RoHS,
WEEE
Safety Declarations ANSI/UL 61010-1:CAN/CSA C22.2 No.
61010-1:IEC 61010-1:CB Scheme
ANSI/UL 61010-1:CAN/CSA C22.2 No.
61010-1:IEC 61010-1:CB Scheme
EMC Declaration IEC 61326-1 IEC 61326-1

10
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Product Overview
Product Overview
The KD Scientific Legato 100 Series is the next
generation of syringe pumps. The series offers
unparalleled ease of use through the high resolution
color touch screen user interface. The full touch screen
interface enables the user to quickly create configurations
and recall them for easy use. The TFT color display
presents all the pump operating parameters on an easy
to view run screen.
The password feature prevents accidental configuration
changes ensuring the programs integrity is retained.
Programs can also be set to be started on a time delay
for unattended operation.
An led light on the front panel makes it easy to see if the
pump is running. Once the pump starts to run, the display
shows the pump is running and the LED turns on. This will
make it easy to see the pump’s status.
The pumps are versatile and can be interconnected through
the RS485 interface. Legato 100 Series models can be
mixed and matched in the daisy chain offering maximum
flexibility. Up to 99 pumps can be linked together through
the RS485 interface. This interface is easy to use provided
each pump is assigned its own unique pump address.
For limited laboratory space the Legato 100 Series can
be placed on its side to reduce the footprint by 55%.
The display also tilts with the change to allow the user to
operate the pump vertically.
Notifications and error messages are displayed for the
user to acknowledge, eliminating any guesswork about
problems.
Flow performance is optimized with a small step angle
stepping motor that drives a precision lead screw and
pusher block. The syringe mechanism is easy to use and
securely holds the syringes for smooth flow performance.
Advanced microstepping techniques are employed
to further reduce the step angle to eliminate flow
pulsation. The accuracy is ±0.5% (Legato 100, 101,
110 and 111), or ±0.35% (Legato 180) with 0.05%
reproducibility. A wide dynamic flow range can be
programmed into the pump. Flow rates are selectable
with user selected engineering units from ml, µl, nl pl, and
hours, minutes and seconds.
Up to 30 lbs of linear force is available. This force is
user adjustable to ensure the right force is applied for the
various syringe sizes.
The superior design of the full metal chassis provides
noise isolation and anti-vibration features for increased
reliability. The spill dam is designed to prevent fluids
from ingressing into the sealed display. All syringe
racks are hardened rolled steel and will not deform with
pressure.

11
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Product Overview
Easy external connections to a computer or other control
devices are made through USB interface. Simple ASCII
commands make communication with the pump easy.
For direct control of the pump, the user can use the I/O
interface (15pin Dsub). A trigger output and a trigger
input in for external events such as a process parameter
is met. The footswitch input will allow the control of the
pump through an external device. The unit also has an
output for run indication allowing connection to a remote
light.
Single or dual syringes models are available. Complete
flexibility is offered with the user able to use most
manufacturer’s syringes with sizes ranging from 0.5 ul to
60 ml.
The infuse/withdraw programmable models Legato
(110, 111 and 180) offer maximum flexibility and
capability for configuring and running different programs.
Up to 2 programs of 50 steps each can be configured
and stored in the unit, and recalled quickly with the
touch of a button. The programs are identified with a
15 character alphanumeric name for easy identification
and can be stored on the computer and downloaded at
future dates. A relative clock is available to start and stop
programs based on time. Extensive programming options
allow the user to duplicate configurations, append one
configuration to another, rename programs, delete
programs, export programs to a PC or export a program
to another pump. This feature can ensure consistency and
accuracy of multiple pumps.
Preprogrammed profiles include Constant Rate and
Ramp. These standard profiles make configuration easy
to set up. If more complexity is needed the user can select
from advanced programming options including:
•Time delays
•Repeating steps
•Stopping the pump
•Triggering the pumps TTL output
•Accepting an event input in such as a user
touch
•Events can trigger the pump to withdraw or infuse
The pump Information/setup screen shows the pump’s
software version, and lubrication intervals. The pump’s
alarm configurability includes alarms for near end of run
(user selectable), completion of run, power up, keypad
clicks and stall detection.
Pump Models
The Legato 100 Series is available in 5 different
configurations.
Legato 100 – Single syringe infuse only.
Legato 110 – Single syringe infuse/withdraw with
program option for 2 programs, 50 steps each..
Legato 101 – Dual syringe infuse only. Syringe size
0.5 µl to 10 ml.
Legato 111 – Dual syringe infuse/withdraw with program
option for 2 programs, 50 steps each.
Legato 180 – Dual syringe infuse/withdraw optimized for
low flow with program option for 2 programs, 50 steps
each. Syringe size 0.5 µl to 10 ml.

12
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Product Overview
LEGATO 100 SERIES TOUCHSCREEN BUTTON REFERENCE
The following buttons are part of the KDS Legato 100 Series software:
Settings: Access the settings menu used to adjust
the following parameters: Audible Alarms, Pusher
Block Force, Display Brightness, etc.
Fast Forward: Advances the Pusher Block in the
forward (infuse) direction (press & hold).
Fast Reverse: Advances the Pusher Block in the
reverse (withdraw) direction (press & hold).
Accept: Used to accept the parameters/settings on
a screen and return to previous screen in the menu.
Cancel: Used to cancel any changes/entries on a
screen and return to the previous screen in the menu.
Set Delay Parameters: Used to set the time
values for delayed operation. This permits the
pump to start automatically at a preset time
interval.
Set Delay Parameters: Indicates the Delay
Timer has been set. The pump will start
automatically.
Run Preview: Access the Run Preview screen
that shows a summary operating parameters.
Run/Resume: Used to Run or Resume a Program.
Re-Run: Clears counters/timers (except the Total
Infused Volume counter) and runs the selected
program from the beginning.
Stop: Used to stop running a Program.
Reset: Resets all counters/timers.
Minimum Value Allowed: Used to
select minimum value allowed.
Maximum Value Allowed: Used to
select maximum value allowed.
Configure: Used to set up the program.
Back: Used to call the Mode and Programs menu.
Page Down: Used to page down in a display list.
Page Up: Used to page up in a display list.
Line Up: Used to scroll up in a display list.
Line Down: Used to scroll down in a display list.
Insert Step: Used to insert a new Step in a
Program while editing/creating the Program. The
new Step is inserted after the Step that is currently
selected.
Delete Step: Used to delete the selected Step in
a Program while editing/creating the Program. To
confirm deletion, you must select the Delete Step
button a second time.
Move Step Up: Moves the currently selected
Step in a Program up one position in the Program
Order (e.g. Step 3 becomes Step 2).
Move Step Down: Moves the currently selected
Step in a Program down one position in the
Program Order (e.g. Step 2 becomes Step 3).
Copy Step: Creates a duplicate (copy) of the
currently selected Step in a Program and inserts
it as the last Step in the Program being created/
edited.
Password Protection Off: Indicates that the
pump is currently unlocked. Pressing the button
twice locks the Touchpad.
Password Protection On: Indicates that the
pump is currently locked. Press the button and
enter password to unlock the Touchpad.
File Options: Access the File Options menu that
is used to manage the list of Programs and to
export Program information to an externally linked
pump or to a connected computer.

13
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Product Overview
Selecting An Item From a Menu List
Selecting items is easy with the Legato 100 Series.
Touch the item to select/highlight the item,
then touch again to select it or press
Canceling a Selection
Canceling selections is easy.
Press
Messages
There are different types of messages.
Error messages are shown in yellow.
• Critical
• Run--mode
• Program
• Set-up
Notices, alarms, confirming, and verified
messages are show in green.
• Alarms
• Confirm
• Verified
Entering Values
Entering new values is easy.
Use the keypad to enter the number
Backspace Key
Decimal point
Accept the value and go back up to the
previous screen.
Cancel and go back to the previous screen.

14
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Product Overview
OPERATING INSTRUCTIONS
POWER CONNECTIONS
1. Plug the power supply into the pump.
2. Connect to a properly rated line cord. The Legato
100 Series power supply has a DC power
input connector on the rear of the unit. The operating
range for the Legato 100 Series series is 100 – 240
VAC, 50-60 Hz.
3. Turn on the main power switch.
!CAUTION: Do not connect to
firewire ports on a computer.
Damage may occur to the
pump or computer.
Half Nut Release Knob
Guide Rods
Pusher Block
Lead Screw
Syringe Barrel Clamp
Syringe Holder
Bracket Clamp Knobs
(only available on Legato 110,
111 & 180)
Run / Fault LED
User Interface
Touchscreen
Protective Antiglare
Close up view of the Legato 110 rear connections
(connections vary depending on model type)
Foot Switch
I/O Connector
USB Serial Input
(Type B)
RS-485 Connection
(Pump to Pump)
Main Switch
DC Power Input
*Power Source -
When powering a pump
in a chain through an RS-
485 cable, toggle switch
to RS-485. Only one
pump can be powered
from the master through
the RS-485.
Legato 110 syringe pump shown above

15
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Product Overview
KDS Legato 100 Series User’s Manual
5. Screw down the syringe clamp knob (6) until the
syringe barrel clamp (10) is tight against the
syringe barrel. Do not overtighten, especially
when using glass syringes.
6. Secure the syringe flanges by pushing the retaining
brackets (4,5) tightly against the syringe flanges
using the bracket clamping knobs (7,8). The
syringe plunger should be secured by the pusher
block retaining bracket and the flange of the
syringe should be secured by the syringe holder
block retaining bracket.
7. Tighten the mechanical stop collars (9) on the
pump to prevent excess pusher block travel.
NOTE: The syringe barrel clamp (10)
[single only] may need to be flipped
depending on your syringe size. To do so,
completely unscrew and remove the syringe
barrel clamp knob from the syringe block.
Then remove lock nut (11). Flip the bracket
and reassemble.
SYRINGE LOADING 25
4
13
9
7
8
10
6
> 30 cc syringes
✐NOTE: For infuse/withdraw models, make sure
the plunger is secured in the retaining bracket
and the barrel of the syringe is secured in the
retaining bracket.
The Legato 100 Series can accommodate up to two
syringes. Depending on the mechanism, any type of syringe,
glass, plastic or stainless steel can be used. The pump can
accommodate from 0.5 µl to 60 ml single syringe and 0.5
µl to 10 ml dual syringe. The syringe barrel clamp should be
flipped for syringes smaller than 30 ml. For syringes larger
than 30 ml, the syringe clamp should be used as shown.
1. Squeeze the release button (1) and move the
pusher block (2) to the approximate length of the
syringe.
2. Unscrew the bracket clamping knobs (7,8) to
loosen the retaining brackets (4,5) on the pusher
block (2) and the syringe holder block (3).
3. Unscrew the syringe barrel clamp (10) by
unscrewing the syringe barrel clamp knob (6).
4. Place the syringe on the syringe holder block (3).
Ensure the barrel flange is within the syringe holder’s
block’s retaining bracket (5) and the plunger flange is
in the pusher block’s retaining bracket (4).
< 30 cc syringes
11
!CAUTION: Be sure mechanical stop
collars are positioned properly to prevent
the plunger from bottoming out on glass
syringes.
NOTE: Syringe brackets (4 & 5) are only
available on Legato 110, 111 & 180. 10
Dual Syringe
Clamp
✐
✐

16
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Configuring the KDS Legato 100 Series
If you select infuse/withdraw or
withdraw/infuse mode the configuration
screen will have the continuous mode selection.
(Press to toggle on/off)
Infuse/Withdraw models only. Continuous flow is an
option on the display. In continuous mode, the pump
will infuse then withdraw continuously or vice versa
depending on which mode is selected. The pump will
switch to withdrawal based on the target volume entered.
The total infuse volume will be displayed on the run
screen as well as total elapsed time.
The KDS Legato 100 Series program is easy to set up.
The Legato 100 Series is easily configured. When
the unit is powered up, the Configuration Screen
will be displayed.
Configuring the KDS Legato 100 Series
Select Mode
Select Flow Rates
Select Syringe Manufacturer Size
Select Target Volume
Fast Reverse Fast Forward
Starts Pump
& Jumps to
Run Screen
Preview
Program
Jumps to
Run Screen
General
Pump
Parameter
Set-Up
Mode Selection
In the Configuration Display select mode
(available modes depend on the pump model)
• Infuse
• Withdraw
• Infuse/Withdraw
• Infuse/Withdraw (continuous)
• Withdraw/Infuse
• Withdraw/Infuse (continuous)
• Custom program
The Mode Selection Screen will be displayed. The
available modes will be displayed based on the
model of syringe pump.
Press the Mode to select View programs up a page
View the programs down a page
Views up one program
Views down one program
Press and hold to activate
KDS Legato 100 Series User’s Manual

17
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Configuring the KDS Legato 100 Series
Syringe Selection
1. In the Configuration Display select the
syringe button.
2. Select the manufacturer type. Then the available
syringe sizes will be displayed. In this example
we have selected a KDS 10ml Glass Syringe.
Press
Press
Press to enter the inside
diameter of the syringe
Custom Syringe Definition Display
Press to enter the total
syringe volume
Press to
change the
units (µl,
ml)
3. For a Custom Syringe use this selection if
syringe manufacturer is not listed.
Press to enter a
custom syringe
!CAUTION: BE SURE TO LOWER THE FORCE VALUE WHEN USING GLASS SYRINGES.

18
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Configuring the KDS Legato 100 Series
2. Depending on the Mode, the appropriate
set rate screen will be displayed. To change the
units, press the units.
3. In the Units Selection Display select the volume
and the time base.
Flow Rate Selection
1. In the Configuration Display enter the flow
rate(s). In this example we want to enter
36.003 ml/min.
Press
Enter the flow rate
Press to change the units
Press to select the
minimum volume for the
syringe size selected
Press to select the
maximum volume for the
syringe size selected

19
KDS Legato 100 Series User’s Manual
phone [phone] fax [phone]
e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Configuring the KDS Legato 100 Series
Target Volume/Time Selection
1. In the Configuration Display enter the target
volume or time. In this example we want a
volume of 5 ml.
2. Depending on the Mode either Target Volume
(I/W or W/I) will appear or Target Volume or
Target Time Selection (I only or W only).
3. To change the units, press the units.
4. In the Units Selection Display select the
volume units.
✐NOTE: For time enter h:m:s. or press
again to select secs. Time is accurate to
+/- 0.01secs. Remember to enter the :
✐NOTE: Target time is only allowed in
infusion only or withdrawal only modes.
Press to select target volume
Press to select the
maximum volume
for the syringe
size selected
Press to select target time
Press to change the units
Press Here