KD Scientific KDS LEGATO 100 User manual

KDS Legato 100 Series
USER’S MANUAL
Publication 5617-006 Rev 2.0
www.kdscientific.com
KDS LEGATO 100 SERIES

5617-006DC Rev 1.0
Declaration of Conformity
Manufacturer: KD Scientific, Inc.
84 October Hill Road
Holliston, Massachusetts 01746-1388, U.S.A.
Phone: [phone]
We herewith declare that the following product:
Product Name: KDS Legato 100 Series Syringe Pump Series
Model No.: KDS Legato 100 Series - Catalog # 7881xx
KDS Legato 950 series- Catalog # 7889xx
To which this declaration relates, is in conformity with the applicable EC Directives, harmonized standards, and
other normative requirements:
Application of Council Directive(s):
2006/95/EC Low Voltage Directive
2004/108/EC Electromagnetic Compatibility Directive
2011/65/EU RoHS Directive
2012/19/EU WEEE Directive
Standard(s) to which conformity is declared:
Safety: IEC 61010-1:2010 (3rd Edition)
UL 61010-1:2012 (3rd Edition)
CSA C22.2# 61010-1:2012 (3rd Edition)
Emissions/Immunity: IEC 61326-1:2013
FCC 47CFR 15B:2014, Class B
EMC and Safety compliance were evaluated by Intertek/ETL Semko
Reference test reports file numbers: 100602821 Box-001, -001a
100179785 Box –001, -003
100179785 Box –005a, -005b
I, the undersigned, hereby declare that the equipment specified above conforms to the above Directive(s) and Standard(s).
Date: July 10, 2015
Signed:
Harvard Bioscience Global Director
Manufacturing and Process Engineering

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KDS Legato 100 Series User’s Manual
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KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
SUBJECT PAGE NO.
Table of Contents
General Information ................................................................................................. 6
Safety Information............................................................................................................................ 6
Warranty and Repair Information.................................................................................................. 7
Manual Description ................................................................................................................ 7
Warranty.................................................................................................................................. 7
Repair Facilities and Parts ...................................................................................................... 7
Serial Numbers ....................................................................................................................... 7
Calibrations ............................................................................................................................. 7
Specifications ................................................................................................................................... 8
Product Overview ...................................................................................................10
Pump Models .................................................................................................................................11
Legato 100 Series Touchscreen Button Reference.....................................................................12
Selecting an Item from a Menu List ..............................................................................................13
Messages .......................................................................................................................................13
Entering Values ..............................................................................................................................13
Operating Instructions ...................................................................................................................14
Power Connections........................................................................................................................14
Syringe Loading.............................................................................................................................15
Configuring the KDS Legato 100 Series................................................................16
Selection Mode..............................................................................................................................16
Syringe Selection ...........................................................................................................................17
Flow Rate Selection .......................................................................................................................18
Target Volume/Time Selection .....................................................................................................19
Previewing the Configuration .......................................................................................................20

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KDS Legato 100 Series User’s Manual
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KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
Configuring the Pump’s General Parameters..............................................................................20
Setting the Audible Alarms...................................................................................................21
For Near End of Run Selection ............................................................................................22
Locking the Touchscreen.......................................................................................................23
Setting the Force Limits..........................................................................................................24
Setting the Display Brightness ..............................................................................................25
Setting the Pump Address.....................................................................................................26
Setting the RS 232 Baud Rate .............................................................................................26
Resume Run on Power Loss ..................................................................................................27
Setting and Viewing Pump Information...............................................................................27
Operating the KDS Legato 100 Series..................................................................28
Running the Pump ..........................................................................................................................29
Custom Program Workflow ..........................................................................................................30
Step-by-Step Operating Example ........................................................................................31
Configuring Custom Programs......................................................................................................32
Configuring New Custom Program..............................................................................................32
Setting a Delay...............................................................................................................................34
Setting a Delay for Program Operation..............................................................................34
Creating a Custom New Program................................................................................................35
To Create a New User-Defined Program ...........................................................................35
Modifying an Existing/User-Defined Program............................................................................37
Changing the Order of Steps in a Program........................................................................37
Deleting a Step from a Program..........................................................................................37
Defining Program Step Parameters ..............................................................................................37
Pump Operation Profiles ...............................................................................................................38
Constant Rate Profile.............................................................................................................39
Ramp Profile...........................................................................................................................40
SUBJECT PAGE NO.

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KDS Legato 100 Series User’s Manual
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KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
Delay......................................................................................................................................41
Repeat Step ...........................................................................................................................41
Stop ........................................................................................................................................42
I/O Event Triggers ................................................................................................................43
I/O (OUT) Trigger (OUT)........................................................................................................44
I/O (IN) Event ............................................................................................................................45
Managing Legato 100 Series Programs.....................................................................................46
Saving a Copy of a Program...............................................................................................46
Renaming a Program ............................................................................................................46
Appending a Program..........................................................................................................47
Deleting a Program...............................................................................................................47
Program Export and Import ..........................................................................................................48
Transfer a Program between Pumps....................................................................................48
Exporting a Program to an External Computer ..................................................................49
Importing a Program from an External Computer..............................................................49
External Pump Control...................................................................................................................50
Setting Up HyperTerminal....................................................................................................50
Pump Chain Commands................................................................................................................51
System Commands................................................................................................................52
Run Commands .....................................................................................................................54
Rate Commands ....................................................................................................................54
Volume Commands...............................................................................................................55
Time Commands....................................................................................................................56
Digital I/O Commands.........................................................................................................57
Internal Commands...............................................................................................................57
Error Codes ....................................................................................................................................58
SUBJECT PAGE NO.

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KDS Legato 100 Series User’s Manual
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KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
Table of Contents
Appendices ..............................................................................................................60
Appendix A: Syringe Volume/Diameter Reference Table.........................................................60
Appendix B: Min/Max Flow Rates..............................................................................................61
Appendix C: External Connections..............................................................................................63
User I/O Connector Specifications.....................................................................................63
Utilizing USB Interface..........................................................................................................64
RS-485 ...................................................................................................................................66
Appendix D: Maintenance ...........................................................................................................67
Maintenance.....................................................................................................................67
Upgrading Legato 100 Series Software........................................................................68
Troubleshooting................................................................................................................69
Appendix E: Ordering Information ..............................................................................................70
SUBJECT PAGE NO.

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KDS Legato 100 Series User’s Manual
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KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
General Information
SAFETY INFORMATION
Please read the following safety precautions to ensure proper use of your syringe pump. If the equipment is used
in a manner not specified, the protection provided by the equipment may be impaired.
To Prevent Hazard or Injury:
USE PROPER POWER SUPPLY
The pump is supplied with an approved power supply and line cord. To maintain the safety integrity of the device,
use only the following power supplies:
Cui Inc. Globtek Inc.
Model: 3A – 242DB24 Model: GT–41062–T3
Output: 24V – 1.0A Output: 24V – 0.75A
Input: 100–240V~50–60 Hz 0.8A Input: 100–240V~50–60 Hz 0.6A
USE PROPER LINE CORD
Use only the specified line cord for this product and make sure line cord is certified for country of use. The
operating voltage range for the KDS Legato 100 Series is 12-30 VDC. The universal power supply operating voltage
range is 100-240 VAC, 50-60 Hz.
GROUND THE PRODUCT
This product is grounded through the grounding conductor of the power cord. To avoid electric shock, the
grounding conductor must be connected to earth ground. Before making any connections to the input or output
terminals of the product, ensure that the product is properly grounded.
MAKE PROPER CONNECTIONS
Make sure all connections are made properly and securely. Any signal wire connections to the unit must be no longer than 3
meters.
OBSERVE ALL TERMINAL RATINGS
Review the operating manual to learn the ratings on all connections.
AVOID EXPOSED CIRCUITRY
Do not touch any electronic circuitry inside of the product.
AVOID PINCH HAZARD
A pinch hazard may exist between the pusher block and end blocks. Avoid placing fingers between these points
while the pump is running.
DO NOT OPERATE WITH SUSPECTED FAILURES
If damage is suspected on or to the product do not operate the product. Contact qualified service personnel to perform
inspection.
ORIENT THE EQUIPMENT PROPERLY
Do not orient the equipment so that it is difficult to operate the disconnection device.
PLACE PRODUCT IN PROPER ENVIRONMENT
Review the operating manual for guidelines for proper operating environments.
OBSERVE ALL WARNING LABELS ON PRODUCT
Read all labels on product to ensure proper usage.
Protective Ground
Terminal
CAUTION
Refer to Manual
!

7
KDS Legato 100 Series User’s Manual
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e-mail info@kdscientific.com web www.kdscientific.com
KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
WARRANTY AND REPAIR INFORMATION
Manual Description
This manual is designed to provide all operational and program information required to operate and maintain the
KDS Legato 100 Series pumps. The functions and features are described in the Technical Specifications section.
Warranty
KD Scientific warranties this instrument for a period of one year from date of purchase. At its option, KD Scientific will
repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty does not extend
to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends only to
the original consumer purchaser.
IN NO EVENT SHALL KD SCIENTIFIC BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some
states do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or
exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS
FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied
warranty, so the above limitation may not apply to you.
If a defect arises within the warranty period, promptly contact KD Scientific, 84 October Hill Road, Holliston,
Massachusetts 01746 at 1–508-429-6809. Email address is info@kdscientific.com. Goods will not be accepted
for return unless an RMA (returned materials authorization) number has been issued by our customer service
department. The customer is responsible for shipping charges for non-warranty repairs. Please allow a reasonable
period of time for completion of repairs or replacement. If the unit is replaced, the replacement unit is covered only
for the remainder of the original warranty period dating from the purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary from state to state.
Repair Facilities and Parts
KD Scientific stocks replacement and repair parts. When ordering, please describe parts as completely as possible,
preferably using a part number obtained from our Customer Service department. If practical, enclose a sample part or
sketch. We offer a complete reconditioning service.
Serial Numbers
All inquiries concerning our product should refer to the serial number of the unit, located on the rear panel.
Calibrations
All electrical apparatus are calibrated at rated voltage and frequency. While the flow and volume will stay
calibrated, the peak pressure may vary. KD Scientific recommends an annual calibration of the pump.
!CAUTION: For research use only. Not for clinical use on patients.
!CAUTION: REFER TO SAFETY INFORMATION AND SETTING UP THE KDS LEGATO
100 SERIES BEFORE PLUGGING IN THE PUMP.

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KDS Legato 100 Series User’s Manual
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KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
Specifications Legato 100, 101, 110, 111 Legato 180
Accuracy ± 0.5% ± 0.35%
Reproducibility ± 0.05% ± 0.05%
Syringes (Min./Max.) 0.5 µl / 60 ml (10 ml dual) 0.5 µl / 10 ml
Flow Rate:
Minimum (0.5 µl syringe) 1.26000 pl/min 0.54000 pl/min
Maximum (10 ml syringe) 26.0170 ml/min 11.7027 ml/min
Maximum (60 ml syringe) 88.4040 ml/min N/A
Display 4.3” WQVGA TFT Color Display
with Touch Screen
4.3” WQVGA TFT Color Display
with Touch Screen
Non-Volatile Memory Stores all settings Stores all settings
Connectors:
RS-485 IEEE-1394, 6 pos IEEE-1394, 6 pos
USB Type B Type B
I/O & TTL 15 pin D-Sub Connector 15 pin D-Sub Connector
Footswitch mini phone jack mini phone jack
Linear Force (Max) 13.6 kg (30 lbs) @ 100% Force Selection 13.6 kg (30 lbs) @ 100% Force Selection
Drive Motor 0.9° Stepper Motor 0.9° Stepper Motor
Motor Drive Control Microprocessor with 1/16 microstepping Microprocessor with 1/16 microstepping
Number of Microsteps
per one rev. of Lead Screw
15,360 20,480
Step Resolution 0.069 µm/µstep 0.031 µm/µstep
Step Rate:
Minimum 27.5 sec/µstep 27.5 sec/µstep
Maximum 26 µsec/µstep 26 µsec/µstep
Pusher Travel Rate:
Minimum 0.15 µm/min 0.02 µm/min
Maximum 159.00 mm/min 71.55 mm/min
Input Power 12-30 VDC 12-30 VDC
Input Power Connection 2.5mm ID x 5.5mm OD male plug 2.5mm ID x 5.5mm OD male plug
Power Supply 100-240 VAC, 50-60 Hz, 8 Watts Universal
Power Supply, Use Only a KD Scientific
Approved Power Supply and Line Cord
100-240 VAC, 50-60 Hz, 8 Watts Universal
Power Supply, Use Only a KD Scientific
Approved Power Supply and Line Cord
Dimensions 22.6 x 19.05 x 15 cm (9 x 7.5 x 5 in) 22.6 x 19.05 x 15 cm (9 x 7.5 x 5 in)
Weight 2.66 kg (5.9 lbs) 2.66 kg (5.9 lbs)

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KD Scientific Legato 100 Series Syringe Pump User’s Manual
Publication 5617-006-REV-2.0
General Information
Specifications Legato 100, 101, 110, 111 Legato 180
Atmospheric Specifications:
Operating Temperature 4°C to 40°C (40°F to 104°F) 4°C to 40°C (40°F to 104°F)
Storage Temperature -10°C to 70°C (14°F to 158°F) -10°C to 70°C (14°F to 158°F)
Operating Humidity See Chart Below See Chart Below
Storage Humidity 20% to 80% RH, non condensing 20% to 80% RH, non condensing
Method of Operation Continuous Continuous
Classification Class I Class I
Pollution Degree 1 1
Installation Category II II
Supplier Name KDS KDS
Supplier Address 84 October Hill Rd., Holliston, MA 01746 84 October Hill Rd., Holliston, MA 01746
Supplier Phone Number [phone] [phone]
Regulatory Certifications CE, ETL, (UL, CSA), CB Scheme, EU RoHS,
WEEE
CE, ETL, (UL, CSA), CB Scheme, EU RoHS,
WEEE
Safety Declarations ANSI/UL 61010-1:CAN/CSA C22.2 No.
61010-1:IEC 61010-1:CB Scheme
ANSI/UL 61010-1:CAN/CSA C22.2 No.
61010-1:IEC 61010-1:CB Scheme
EMC Declaration IEC 61326-1 IEC 61326-1