Kendro HERAsafe KS User manual

Kendra
Operating Instructions
Safety Cabinet HERAsafe KS

Copyright
These operating instructions are protected by copyright. Rights resulting the¬
reof, particularly reprint, photomechanical or digital postprocessing or reproduc¬
tion, even in part, are only allowed with the written consent of Kendro Laboratory
Products GmbH.
This regulation does not apply to reproductions for in-plant use.
Trademarks
HERAsafe® and Heraeus are registered trademarks of Kendro Laboratory Pro¬
ducts GmbH. All other trademarks mentioned in the operating instructions are
the exclusive property of the respective manufacturers.
Kendro Laboratory Products GmbH
Robert-Bosch-StraBe 1
D - 63505 Langenselbold
Germany

••Kendro
Operating Instructions Safety Cabinet HERAsafe KS
Contents
1. General notes.6
1.1 Safety instructions.6
1.2 Warranty.7
1.3 Explanation of symbols.7
1.3.1 Symbols used in the operating instructions.7
1.3.2 Symbols on the device.8
1.4 Use of the device.9
1.4.1 Correct use.9
1.4.2 Incorrect use.9
1.5 Standards and safety regulations.10
2. Delivery.11
2.1 Standard components.11
2.2 Acceptance inspection.11
3. Installation.12
3.1 Ambient conditions.12
3.2 Room ventilation.12
3.3 Correct location.13
3.4 Installation in series.13
3.5 Transport.13
4. Description.14
4.1 Overall view.14
4.2 Safety system.16
4.3 Filter system.17
4.4 Controls and display.18
4.5 Sample chamber access.19
4.6 Device interfaces.20
4.7 UV lamp unit.22
4.8 Working area.22
5. Start-up.23
5.1 Initial operation.23
5.2 Installing unit and accessories.23
5.3 Leveling the cabinet.24
5.4 Activating the remote control.24
5.5 Power supply connection.25
5.6 RS 232 interface connection.26
5.7 UV connection.26
5.8 Installation test.27
6. Handling and control.28
6.1 Display.28
6.1.1 Functions of the display components.28
6.1.2 Display in OFF mode.29
6.1.3 Display in work mode.29
6.1.4 Display and function after a power failure.29
6.1.5 Failure messages.29
6.2 Description of the operating modes.30
6.3 Remote control.32
6.3.1 Basic functions.32
6.3.2 Moving the front window to the working position.33
6.3.3 Silencing the audible alarm signal .34
6.3.4 Switching the illumination on and off.34
6.3.5 Activating and deactivating the internal power supply.34
6.3.6 Displaying the UV disinfection time.35
6.3.7 Activating and deactivating the
potential-free contact (optional).35

Operating Instructions Safety Cabinet HERAsafe KS
••Kendro
Contents
6.3.8 Switching the cabinet to OFF mode.35
6.3.9 Setting the time.36
6.3.10 Displaying the downflow velocity.37
6.3.11 Displaying the total operating hours.37
6.3.12 Displaying the Kendro Performance Factor.38
6.3.13 Setting and activating the timer.39
6.3.14 Deactivating the timer.40
6.3.15 Setting the UV disinfection time.41
6.3.16 Starting the UV disinfection.41
6.3.17 Cancelling the UV disinfection.42
6.3.18 Activating the stop watch.42
6.4 Pilot switch.43
6.4.1 Moving the front window.43
6.4.2 Silencing the audible alarm signal.44
6.4.3 Switching the device to OFF mode.44
7. Operation.45
7.1 Hygiene preparations for the sample chamber.45
7.2 Preparing the sample chamber.45
7.3 Response to failure messages.45
7.4 Work rules.46
8. Shut-down.47
8.1 Interrupting an operation.47
8.2 Shutting the unit down.47
9. Cleaning and decontamination.48
9.1 Decontamination procedure.48
9.2 Wipe/spray disinfection.48
9.3 UV disinfection after a wipe/spray disinfection.50
9.3.1 UV disinfection using the integral UV lamps.50
9.3.2 UV disinfection using a mobile UV device.50
9.4 Disinfection with formaldehyde.50
9.5 Cleaning the exterior surfaces.51
9.6 Cleaning the floorpan.51
10. Maintenance.52
10.1 Inspection.52
10.2 Service.52
10.2.1 UV lamps.52
10.2.2 Sample chamber illumination.53
10.2.3 Replacing the front cover seal.53
10.3 Retrofitting and repairs.54
11. Disposal.55
11.1 Disposal procedure.55
12. Technical data.56
13. Device log.60
14. C ertificate of decontamination
61

••Kendro
Operating Instructions Safety Cabinet HERAsafe KS
Figures
Fig. 1 Device locations in a room.13
Fig. 2 Lift points.13
Fig. 3 Overall view.15
Fig. 4 Filter system with downflow filter and exhaust air filter.17
Fig. 5 Controls and display.18
Fig. 6 Display with remote control sensor.18
Fig. 7 Access through front cover and front window.19
Fig. 8 Device interfaces.21
Fig. 9 UV lamp unit.22
Fig. 10 Working area on the workplate.22
Fig. 11 Installing accessories.23
Fig. 12 Inserting the batteries.24
Fig. 13 RS 232 interface connection.26
Fig. 14 UV connection.26
Fig. 15 Functions of the display components.28
Fig. 16 Basic functions of the remote control.32
Fig. 17 Basic functions of the pilot switch.43
Fig. 18 Front cover seal replacement.53

Operating Instructions Safety Cabinet HERAsafe KS
IKendro
1. General notes
The following are the addresses of the international Kendro
Sales Organisations.
Postal address Germany
Kendro Laboratory Products GmbH
Robert-Bosch-StraBe 1
D - 63505 Langenselbold
Enquiries from Germany
Phone
Sale 0800 1 536376
Service 0800 1 112110
Fax
Sale/Service 0800 1 112114
E-Mail [email protected]
Enquiries from Europe, Middle East
and Africa
Phone + 49 (0) 6184/ 90-6940
Fax [phone]
E-Mail [email protected]
1.1 Safety instructions
These safety instructions describe the safety features of the HERAsafe series
and apply to the models KS 9/12/15/18.
The safety cabinet has been manufactured in keeping with the latest techno¬
logical developments and has been tested before delivery for its correct function.
It may, however, present potential hazards if it is not used according to the in¬
tended purpose or outside of operating parameters. Therefore, the following pro¬
cedures must always be observed:
• The safety cabinet must be operated only by trained and authorized personnel.
• For any operation of this unit, the operator must prepare clear and concise
written instructions in the language of the operating and cleaning personnel
based on these operating instructions, applicable safety data sheets, plant
hygiene guidelines, and technical regulations, in particular:
• which decontamination measures are to be applied for the cabinet and
accessories,
• which protective measures apply while specific agents are used,
• which measures are to be taken in the case of an accident.
• Repairs to the device must be carried out only by trained and authorized expert
personnel.
• The contents of the operating instructions are subject to change without
further notice.
• Concerning translations into foreign languages, the German version of these
operating instructions is binding.lease
• Keep these operating instructions close to the unit so that safety instructions
and important information are always accessible.
• Should you encounter problems that are not detailed adequately in these ope¬
rating instructions, please contact Kendro Laboratory Productsimmediate-
ly for your own safety.
Postal address USA
Kendro Laboratory Products Inc.
275 Aiken Road
Asheville, NC 28804
USA
Enquiries from North America
Phone [phone]
Fax [phone]
E-Mail [email protected]
Enquiries from Latin America
Phone [phone]
Fax [phone]
E-Mail [email protected]
Enquiries from Asia Pacific
Phone [phone]
Fax [phone]
E-Mail [email protected]
6

••Kendro
Operating Instructions Safety Cabinet HERAsafe KS
1. General notes
1.2 Warranty
Kendro Laboratory Products warrant the operational safety and functions of the
safety cabinet only under the condition that:
• the device is operated and serviced exclusively in accordance with its intended
purpose and as described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by Kendro
Laboratory Products are used,
• inspections and maintenance are performed at the specified intervals,
• an installation test is performed prior to the initial operation of the device and
that a repeat test is performed on the occasion of all inspections and repairs.
The warranty is valid from the date of delivery of the device to the operator.
1.3 Explanation of symbols
1.3.1 Symbols used in the operating instructions:
WARNING!
is used if non-observance may cause serious
or even lethal injuries.
CAUTION!
is used if non-observance may cause medium
to minor injuries or damage.
NOTE!
is used for hints and useful information.
RECYCLING!
Valuable raw materials can be reused.
A
A
o=
7

Operating Instructions Safety Cabinet HERAsafe KS
IKendro
1. General notes
1.3.2 Symbols on the device:
Observe operating instructions
A
A DANGER
HIGH VOLTAGE!
TURN POWER OFF
AND DISCONNECT
POWER SUPPLY
BEFORE SERVICING
TO BE OPENED BY
AUTHORIZED
PERSONAL ONLY
High Voltage (electrical cover)
Biohazard (left front of unit)
NSF symbol (illumination box cover)
2 xT 5A
ETL symbol (illumination box cover)
TSA note (outlet cap in sample chamber)
RS 232
RS 232 interface (connection label)
8

••Kendro
Operating Instructions Safety Cabinet HERAsafe KS
1 .
General notes
1.4
Use of the device
1.4.1 Correct use
The safety cabinet is a laboratory device for installation and operation in micro¬
biological and biotechnical laboratories of safety levels 1,2, and 3. It has been
designed as a Class II safety cabinet, Type A/B3, in accordance with NSF, Stan¬
dard 49/1992.
Depending on the hazard level of the agents involved, the operator must pre¬
pare in writing appropriate decontamination procedures for the device and the
accessories used in the sample chamber.
Prior to the initial operation of the cabinet, the operator should perform an in¬
stallation test. The test result must be documented by a test report. The cabi¬
net must only be released for operation if it is in compliance with the operating
parameters specified by Kendro Laboratory Products.
After any changes to the installation conditions or after any modification to the
technical system, a repeat test must be performed and the test result must be
documented by a test report that shows that all operating parameters are in com¬
pliance with those specified by Kendro Laboratory Products.
1.4.2 Incorrect use:
The safety cabinet must not be used in laboratories that do not comply with the
requirements of safety levels 1,2, and 3.
The unit must not be operated as a Class II safety cabinet, Type A/B3, if:
• no repeat test is performed after changes to the installation conditions or after
modifications to the technical system,
• the alarm system of the device has issued a failure message and the cau¬
se for the failure has not been repaired.
The alarm system must not be tampered with or disabled. If alarm system com¬
ponents heve been removed or disabled for service or repairs, the unit must only
be released for operation if all alarm system components are functioning again
properly.
The filters installed in the device are not capable of separating gaseous sub¬
stances. Therefore, do not work with or store substances in the device:
• that in quantity or concentration are toxic,
• if a reaction with other substances may result in hazardous toxic concentrations
or formation of toxic gases,
• that may form combustible or explosive mixtures in combination with air.
9

Operating Instructions Safety Cabinet HERAsafe KS
••Kendro
1. General notes
1.5 Standards and safety regulations
The device complies with the safety requirements of the following standards and
guidelines:
USA/Canada
• IEC 1010-1 / UL 3101-1
• NSF, Standard 49/2002
Europe / Middle East / Africa
• IEC 1010-1 / EN 61010-1
• EN 12469/2000
• Low Voltage Directive 73/23 EWG
• EMC Directive 89/336 EWG
Australia / Asia / Pacific
• IEC 1010-1
• AS 2252.2
10

••Kendro
Operating Instructions Safety Cabinet HERAsafe KS
2. Delivery
2.1 Standard components
Delivery for the safety cabinet includes the following:
• safety cabinet,
• remote control,
• drain valve.
• Device documentation:
— operating instructions,
— factory test report.
Optional components and accessories are listed as separate items in the deli¬
very document.
2.2 Acceptance inspection
After the device has been delivered, immediately check the device:
• for completeness,
• for possible damage.
If the delivery is incomplete or if you detect any transport damage to the
device, contact the forwarding agency and Kendro Laboratory Products
immediately.
11

Operating Instructions Safety Cabinet HERAsafe KS
••Kendro
3. Installation
3.1 Ambient conditions
The operational safety and correct function of the unit depend on the location
where it is to be operated. The safety cabinet must be operated only at locati¬
ons that meet the ambient conditions listed below.
Location requirements:
• The electrical system of the device has been designed for an operating height
of up to 6500 ft above sea level.
• The mains power supply outlets should be out of casual reach to prevent ac¬
cidental shut-off. Ideally, the outlets should be installed above the safety
cabinet.
• The flooring of the location must be adequately strong and not flammable.
• The room in which the device is installed must be of adequately height. For
units not connected to an exhaust system, the distance between the exhaust
air opening and the room ceiling must be at least 200 mm (8 in).
• The location must be equipped with an appropriate ventilation system (see
Section 3.2.).
• The temperature within the room must be between 15° C and 40° C (49° F
and 104° F).
• The relative humidity in the vicinity of the device must not exceed 90 %.
NOTE - Ambient conditions!
If ambient conditions vary from those descri¬
bed above, please contact Kendro Laborato¬
ry Products for assistance in installing the
device.
NOTE - Temporary storage!
If the device is stored only temporarily (up to
four weeks), the ambient temperature may be
between -20° C and +60° C (-4° F and +140° F)
at a relative air humidity of up to 90 %. For lon¬
ger storage periods, the location requirements
apply.
3.2 Room ventilation
The room ventilation should preferably be a ventilation system that complies with
the national requirements for the application.
• The inlet air and exhaust air openings of the room ventilation must be located
so that drafts are prevented from impairing the function of the safety cabi¬
net air system.
12

••Kendro
Operating Instructions Safety Cabinet HERAsafe KS
3. Installation
3.3 Correct location
Choose a draft-free location where the safety cabinet does not
interfere with the plant traffic.
Fig. 1 : This figure shows preferred locations for safety cabi¬
nets and unsuiable locations, not in accordance with the safety
requirements.
Unsuitable locations: The locations [1], [2], and [3] are not
suitable because they are exposed to drafts from windows and
doors.
Location [5] is undesirable because it is in range of plant traffic
and within the exhaust air range of a ventilation system [4].
Preferred locations [6], [7], and [8] are correct because they
are in a draft-free section of the room and not exposed to plant
traffic.
3.4 Installation In series
When several devices are to be installed in series, please ob¬
serve the following:
• Make sure that vibrations cannot be transferred between
adjacent units.
• Exterior surfaces of the cabinets must always be accessible
for cleaning and disinfection.
3.5 Transport
Fig. 2 : To prevent the unit from tipping, always transport using
a suitable carrier, even for a transport within a building, and
separate the unit from the stand.
£^j=> NOTE - Lift points
For transport, lift the unit only at the lift points
shown in Figure 2.
Do not allow the weight of the cabinet to rest
on the floorpan!
Fig. 2
Lift points
13

Operating Instructions Safety Cabinet HERAsafe KS
••Kendro
Description
4.1 Overall view
• Fig. 3 : Plenum [5] (assembly containing blowers and filters) with downflow
blower [24] and exhaust air blower [2]. The device downflow filter and the
exhaust air filter are installed with the corresponding blowers. Exhaust air
is released through the opening [1],
• Optional media lines [25] are routed into the sample chamber [20] at the side
of the plenum.
• Electrical box [4] with power supply cable [3]. At the front of the electrical box
are a connection for an RS 232 interface for connecting a PC, as well as two
fuse holders installed.
• Front cover [6] with electrically operated front window [7] and gas strut [22]
for support when open.
• The illumination system [23] with two tubes is part of the front cover assy.
• Side slates [9] with two sealed access openings [19]. These can be equip¬
ped with media valves [13].
• Internal outlets [10] for power supply of accessories and adapter [11] for mobile
UV device.
• Optional UV lamp unit [8] consisting of two UV lamps per side.
• Optional stands [12], adjustable in height (KFS 2) and with fixed height
(KFS 1).
• Workplate segments [15] with optional arm rests [16]. A one-piece workplate
and special workplates are available options.
• Drain valve [17] for installation into floorpan.
• Display [21] with remote control sensor and alarm system LEDs.
• Test hoses for the supply unit [18] at the left side of the sample chamber and
for the exhaust unit [14] at the right side of the sample chamber.
£^j=> NOTE - Test hoses!
Do not remove the two test hoses for checking
downflow and exhaust air.
14

IKendro
Operating Instructions Safety Cabinet HERAsafe KS
4. Unit description
15

Operating Instructions Safety Cabinet HERAsafe KS
IKendro
Unit description
4.2 Safety system
The safety system comprises a combination of protective and alarm systems
that ensure maximum personal and material protection.
Safety systems:
• Vacuum-sealed air system
A vacuum-sealed air system in combination with ULPA filters for downflow
and exhaust air forms the basis of the safety system for personal and ma¬
terial protection.
• Personal protection
Air aspired from the exterior along the entire working opening at a constant
high velocity prevents that:
• agents may leak through the working opening of the chamber.
As the exterior air pressure around the unit exceeds the pressure of the in¬
ternal air system (vacuum sealing), it is ensured that:
• agents cannot be released to the exterior in the case of a leak in the
cabinet housing.
• Material protection
A steady airflow within the air system ensures that:
• a constant downflow allows the ULPA filters to remove contaminants
so that the samples are always surrounded by ultrapure air,
• harmful particles are not carried over through the sample chamber
(protection from cross-contamination).
• ULPA filters
The downflow (i.e. the air circulating within the device) and the exhaust air
(air that is released to the exterior) are cleaned by ULPA filters (ULPA= Ultra
Low Particle)
• Safety lockout
To protect from UV radiation, the optional UV disinfection routine can be run
only if the front opening is closed. During UV disinfection, the front opening
safety lockout is activated and prevents harmful UV radiation from being
emitted from the sample chamber.
Warning system:
• Airflow monitoring
Airflow monitoring determines the velocity of the airflow in the sample chamber
as well as the inflow velocity of the air aspired from the exterior through the
working opening. As soon as airflow velocities move above or below a spe¬
cified safety value, a signal is transmitted to the alarm system.
• Visual and audible alarm system
The warning system constantly monitors the safety-relevant device functions:
• Inflow velocity of the air aspired from the exterior,
• downflow velocity,
• working position of the front window.
If the warning system detects changes to one of these device functions, it
issues:
• an audible and a visual alarm signal.
16

Kendro
Operating Instructions Safety Cabinet HERAsafe KS
Unit description
• Position monitoring
The position sensors monitor the position of the front co¬
ver as well as the movement of the front window; it will in¬
dicate when the front window is in the working position.
• Kendro Performance Factor
The Kendro Performance Factor (PER) is a value that in¬
dicates the safety state of the safety cabinet. This value is
calculated from data determined by the safety system and
from values captured empirically by service personnel
during safety checks. This data is entered into a parame¬
ter list of the control software and interconnected. The result
can be indicated by the display.
4.3 Filter system
Fig. 4: The filter system consists of two ULPA filters [2] and
[5] for the circulating air and for the exhaust air and of a coarse
filter for the aspired air.
ULPA filters: Room air [10] is drawn into the sample cham¬
ber through the working opening. In the air duct, room air and
the downflow within the chamber [7] are then blended to make
up the blend air [9]. The blend air is then:
• filtered proportionally by the downflow filter [5] and supp¬
lied as ultrapure air [6] evenly into the sample chamber of
the device,
• filtered by the exhaust air filter [2] and released as ultrapure
air [1] to the exterior of the device.
Inlet air protection: The air duct between the sample chamber
and the device plenum has an inlet air protection [8] below the
working surface to prevent coarse particles from entering the
plenum where they may impair blower [3] und [4] and filter
functions.
Fig. 4
Filter system with downflow
filter and exhaust air filter
M
co

Operating Instructions Safety Cabinet HERAsafe KS
IKendro
4. Unit description
4.4 Controls and display
Fig. 5: The safety cabinet is equipped with two separate control
elements that operate independently of each other:
• remote control [3],
• pilot switch [1].
The status indicators of the display [2] indicate control opera¬
tions initiated with the control elements.
Remote control: All device functions can be activated and
deactivated easily using the remote control.
Pilot switch: Use to control all basic functions required for the
operation. This switch allows you to operate the safety cabi¬
net if the remote control is not operational or available.
Fig. 6, display: The display [2] can show text or numeric va¬
lues and has 12 LEDs to indicate the current operational state
of the unit.
The display module also houses the sensor system for the re¬
mote control. The pulses transmitted are best received by the
sensor if the distance A between the remote control [5] and the
sensor [3] does not exceed 1m and if the radio signal beam
does not deviate more than 15° horizontally.
The transmission range of the remote control also depends on
the battery state of charge.
Controls and display
Display with remote control
sensor
18

IKendro
Operating Instructions Safety Cabinet HERAsafe KS
4. Unit description
4.5 Sample chamber access
The sample chamber of the device is accessible via two modes:
• Fig. 7: Manual opening of the front cover [2] allows access to the comple¬
te sample chamber width with an opening height C. It is generally needed
for decontamination and introduction of larger accessories.
• The electrically operated front window [1 ] is made of multi-layer safety glass
and integral to the front cover frame. It can be raised to a maximum opening
height B. To access the sample chamber during the work process, the front
window must stay in the work position with opening height A.
CAUTION - Front window movement!
Fig. 7
Access through front cover and
front window
Do not attempt to move the front window
manually as otherwise the motor drive may be
damaged.
NOTE - Front cover lockout!
The front cover is equipped with a safety lock¬
out and can only be opened in standby mode,
i.e. when the front window is completely
closed.
19

Operating Instructions Safety Cabinet HERAsafe KS
••Kendro
Unit description
4.6 Device interfaces
Fig. 8: The standard equipment includes the outlets [11] for internal power supply
as well as the openings [8] on both sides for routing of cables and hoses. All other
supply connections are available as options and may also be retrofitted.
Power supply connection: The connection to the power supply system is achie¬
ved through a cable with grounding plug [2] at the rear of the electrical box.
Internal power supply: The front of the box has an RS 232 interface [3] for the
connection to a PC as well as two fuse holders for 5A miniature fuses:
[4] for (L),
[5] for (N).
£-'y=> NOTE - Electrical data of internal outlets!
Rated voltage: 1/N/PE AC, 115 V / 60 Hz.
Fusing: T 5 A.
Maximum leakage current: 0.5 mA
Other connections: At the rear of the electrical box there are two connections for
a potential-free contact [3] and [4] as well as an RS 232 connection [5] for a PC.
Media valves: There are two feed throughs [8] sealed with screw locks [9] on
each side. These may be used for installation of media valves [7].
NOTE - Inlet pressure!
Inlet presssure for media valves is: max. 6 bar.
Media supply lines: Additionally, media can be supplied into the sample chamber
through three pipes. The inlets [1] are located on top of the housing, the out¬
lets [6] are placed at the sample chamber backpanel.
Disinfection adapter (optional): The disinfection adapter [10] is used to connect
a mobile UV radiation device. The adapter is connected to the device control,
the UV disinfection routine with a mobile UV device may be controlled with the
remote control.
20