Knauer Azura V 2.1S User manual

Valve Drive V 2.1S
User Manual
V6860
HPLC

Note: The contents of this user manual apply for the BlueSha-
dow product. Please submit a request on any article numbers for
BlueShadow.

Table of Contents 3
AZURA V 2.1S User Manual, V6860, Version 2.2
Table of Contents
Note: For your own safety, read the manual and always observe the warnings
and safety information on the device and in the manual.
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Valves and Valve Drives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Valve Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2-Position Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Multiposition Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Valve Drives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Contact Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Operating Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installation Site . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Checking the Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Mounting the Valve onto the Valve Drive . . . . . . . . . . . . . . . . . . . . . . . . 18
Setting the Valve Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Screwing the Adapter Plate onto the Valve Drive . . . . . . . . . . . . . . . . 19
Screwing the Valve onto the Valve Drive . . . . . . . . . . . . . . . . . . . . . . 20

4TableofContents
AZURA V 2.1S User Manual, V6860, Version 2.2
Installing the Sample Loop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Description for PEEK Sample Loop . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Description for Stainless Steel Sample Loop . . . . . . . . . . . . . . . . . . . . 22
Inserting the Needle Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Connecting the Device to the Computer . . . . . . . . . . . . . . . . . . . . . . . . 23
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . 25
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . 26
Connecting the Remote Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
GROUND . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
HOME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
BIN 0 - 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Binary Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Ground . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Operating with the Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Operation with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . 30
Switch On and Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Setting the Valve Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Injecting the Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Application Examples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Valve Positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Valve Position Load (L) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Valve Position Inject (I) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Selecting the Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Backflushing the Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Backflushing the Precolumn . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Functional Tests IQ and OQ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Maintenance and Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Table of Contents 5
AZURA V 2.1S User Manual, V6860, Version 2.2
Valve Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Disassembling the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Orientating the Rotor Seal in the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Valve Drive and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Valves for Drives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Manual Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Power Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Wear Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Legal Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
To whom it may concern
In case you prefer a French language user manual for this product, submit your
request including the corresponding serial number via email or fax to KNAUER:
[e-mail]
[phone]
Thank you.
A qui que ce soit
Si jamais vous préfériez un manuel en francais pour ce poduit contacter
KNAUER par email ou par fax avec le no. de série:
[e-mail]
[phone]
Merci beaucoup.

6 Intended Use
AZURA V 2.1S User Manual, V6860, Version 2.2
Intended Use
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the device
could fail.
Description
2-position valves are used in the HPLC to bring in the sample from the sample
loop in the pumps high pressure flow, so that the sample is transported to the
column. The sample is brought into the sample loop with a syringe or a feed
pump. Multiposition valves serve for fractionating, eluent selection, or sample
selection.
2-position valves have 6 ports and 3 channels (6P/3C), multiposition valves
have 1 channel always.
Operating Ranges
Among others, the device can be used for analytical or purification tasks.
Device Overview
Various types of valves with different operating modes allow the use of these
valves for a variety of applications. It is important that valve drive and valve are
compatible.
You can mount additional manual valves to an optional mounting bracket that
can be fixed with screws on the AZURA modules or other devices. Find further
information on our website www.knauer.net.
Valves and Valve Drives
Valves and valve drives are completely assembled and tested when they leave
the factory.
Legend
1 valve drive with valve
2 valve for drive
3 manual valve
Fig. 1 overview of valves and valve drives
3
1
2

Intended Use 7
AZURA V 2.1S User Manual, V6860, Version 2.2
Front View
The valve drive has a display and a keypad.
Rear View
On the rear of the device there are the power-connection bushing, ground for
grounding the device, connections for external devices, symbols, warning signs
and serial number.
External devices can be connected to the valve drive in three different ways:
via remote connector
via LAN connector within a network
via RS-232 port
Legend
1 multifunction key [L] [>]
2 multifunction key [I] [<]
3 position indicator
4 adapter plate
5 valve for drive
6 bore holes for mounting bracket
Fig. 2 valve drive with 2-position valve
1
2
3
4
56

8 Intended Use
AZURA V 2.1S User Manual, V6860, Version 2.2
Valve Types
Generally, valves can be operated manually through a lever or electrically
through the valve drive. Valves are available as 2-position valves or multiposition
valves
Ports and channels are described by abbreviations to enable the identification of
a specific valve. Example: 6P/3C = 2-position valve with 6 ports and 3 channels.
In case of the multiposition valves, the input/output port is not counted.
Valves are available in stainless steel or PEEK and with 1/8¨ or 1/16¨ connectors.
2-Position Valves
2-position valves are used for injection, column switching, or column backflush-
ing. In case of the 2-position valves, the rotary motions of the electrical valve are
limited to 2 positions with a rotation angle of 60°.
You control 2-position valves either manually through a lever or electrically
through the valve drive.
Legend
1 serial number
2 RS-232 port
3 LAN connector
4 remote connector
5 mains power connection
6 hole for the ground connection
Fig. 3 valve drive
1
2
3
5
4
6
7

Intended Use 9
AZURA V 2.1S User Manual, V6860, Version 2.2
Reed Contact
The reed contact inside the 2-position valve causes a short-circuit contact. This
short-circuit contact can be used as a start signal for the entire analytical system
or as a start signal for a measurement with the detector. The function is to be
set on the receiving device.
Multiposition Valves
Multiposition valves are used to switch fluid streams, e.g. the selection of elu-
ents, columns or for fractionating of eluate. Multiposition valves are employed
for fractioning if the form and the size of the fraction containers should be freely
selectable. By combining up to 8 multiposition valves, up to 120 positions can
be used for fractioning in applications in the preparative HPLC.
You control all multiposition valves electrically through a valve drive. 6P-multi-
position valves can alternatively be controlled manually through a lever.
Legend
1 adapter plate for mounting on the
valve drive
2 port number
3 channel
Fig. 4 scheme of a 2-position valve
1
4
62
53
12 3

10 Intended Use
AZURA V 2.1S User Manual, V6860, Version 2.2
Valve Drives
Valve drives are operated by a 24 V direct current motor. The valve drive and
the valve come factory-set. You can change the valve mode by using the DIP
switches (Dual-Inline Package) on the bottom of the device.
By means of chromatography software or a cable for remote control, certain
ports can be controlled directly on a valve.
switch duration of the valve: 150 ms
There is a matching valve drive for every valve type. 6P/3C valves and 6P-multi-
position valves operate on the same valve drive.
Note: Submit any questions about the possible combinations between valves
and valve drives to the Technical Support of KNAUER.
All valve drives come with a ready-mounted valve (see “Repeat Orders” on
page 43).
Seals
You are to use the seals according to their pressure resistance.
For pressures < 50 bar you can use a bicone seal.
For pressures > 50 bar you use Dynaseal for PEEK capillaries and screw fittings
with ferrules for stainless steel or titanium capillaries.
Legend
1 port number
2 connection between central port and ports 1-6
3 adapter plate for mounting on the valve drive
Fig. 5 scheme of a 6P-multiposition valve
123