Kontron HPLC Detector 430 User manual

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Hi Instruction manual DETECTOR 430 HPLC
"iälllp/' T A B L E O F C O N T E N T S
1. INSTALLATION
1.1 Unpacking
1.2 Checking the instruments *s serial number
1.3 Checking the accessories
1 . 4 C h e c k i n g t h e m a i n s s u p p l y
1.5 Installation site
1.6 Installation
1.6.1 Installation of the lamp module
1.6.2 Installation of the flow cell
1.7 Signal inputs and outputs
page
2
2
2
3
4
5
5
6
2. INSTRUMENT DESCRIPTION
2.1 Specifications
2.2 Construction of the instrument
2.3 Keyboard and display
2.3.1 Display
2.3.2 Function keys
3.
3.1
OPERATION
Initialization
Nfejys//
3.2 Stand-alone operation
3.2.1 Single mode
3.2.2 Dual mode
3.2.3 Lamp energy
3.3 Remote operation
3.3.1 Data transfer
3.3.2 Operating modes
3.3.3 Mode commands
3.3.4 Parameter ranges
3.3.5 Auxiliary functions
3.3.6 Methods programming
3.3.7 Lambda scan measurement
3.3.8 Lambda fix measurement
3.3.9 Lambda ratio measurement
3.3.10 Snapshot measurement
3.3.11 Dual wavelength measurement
4
4
5
6
7
7
8
9
11
13
17
18
20
21
23
25
08.86 0 GENERAL INFORMATION - 1 -

Hi Instruction manual DETECTOR 430 HPLC
T A B L E O F C O N T E N T S ( c o n t . )
4. ERROR MESSAGES
4.1 Error messages in stand alone operation
4 . 1 . 1 S y s t e m e r r o r s
4.2 Error messages in remote operation
4.2.1 System errors in remote operation
4. 2 .2 R e mo t e sp e ci fi c er r or s
4.2.3 Acknowledgement and resetting of system errors
4.2.4 RS interface error data format
page
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5. MAINTENANCE
5.1 Replacing the lamp
5.2 Cleaning the flow cell
5 . 3 S e r v i c e p a r t s f o r t h e fl o w c e l l
6. OPTIONS
7. TECHNICAL INFORMATION
7 . 1 S t a t e s o f T T L o u t p u t s
7 . 2 O p t i c a l fi l t e r p o s i t i o n s
7 . 3 P r i n c i p l e s o f c o m m u n i c a t i o n
7 . 4 B a s i c p r o g r a m f o r L a m b d a - fi x
08.86 0 GENERAL INFORMATION - 2 -

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Hi Instruction manual DETECTOR 430 HPLC
T A B L E O F C O N T E N T S
1. INSTALLATION
page
1.1 Unpacking
1.2 Checking the instruments•s serial number
1.3 Checking the accessories
1 . 4 C h e c k i n g t h e m a i n s s u p p l y
1.5 Installation site
1.6 Installation
1.6.1 Installation of the lamp module
1.6.2 Installation of the flow cell
X^gs
1.7 Signal inputs and outputs
08.86 1 INSTALLATION - 1 -

\{i Instruction manual DETECTOR 430 HPLC
l.i UNPACKING
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Open the box. Hold the cardboard handles and pull out the instrument.
All accessories are shipped inside the.main box. Check the accessories
according to chapter 1.3.
1.2 CHECKING THE INSTRUMENT'S SERIAL NUMBER
The plate with the serial number is mounted on the rear of the instru
ment just below the heat sink.
This number must be identical with the serial number on the delivery
note.
1.3 CHECKING THE ACCESSORIES
The following standard accessories are included in PN 99-00293:
1 M a i n s c o r d ( S c h u c o ) 5 5 - 6 5 6 0 1
1 Instruction manual 95-00184
1 Installation report 84-00046
2 F u s e s 4 A s l o w b l o w ( f o r 1 1 0 V m a i n s i n p u t ) 6 2 - 1 3 0 1 5
1 D u s t c o v e r 9 1 - 0 0 5 5 2
1 A l l e n k e y 3 m m 4 0 - 3 0 0 0 3
1 Lamp module with factory adjusted deuterium lamp 91-00644
1 3 ul Flow cell 91-00540
12.89 1 INSTALLATION - 2 -

Hi Instruction manual DETECTOR 430 HPLC
1.4 CHECKING THE MAINS SUPPLY
The HPLC Detector 430 is factory set for 220V 50/60Hz. For operation
with other mains voltages, the correct voltage must be set on the
voltage selector (see picture below).
Possible voltages: 100, 120, 220 und 240 VAC
The fuses on the voltage selector (1) have to be adjusted according to
the mains supply.
Frequency adjustment for 50/60Hz is not necessary.
Name plate
Voltage selector
- Unplug mains cable
- O p e n t h e s n a p - c o v e r ( A ) b y
ins e rting a sc r ew drive r at the
top on the selector.
- Pull marker wheel (B) and turn it
-to the-required position
- Push (B) back into position
- Pull out both fuse holders (C) and
insert the correct fuses
- Push holders (C) back into posi
tion, obey arrows
- Close snap cover (A)
- Check that the correct voltage is
displayed
08.86 1 INSTALLATION - 3 -

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H i I n s t r u c t i o n m a n u a l d e t e c t o r 4 3 0 H P L C
1.5 INSTALLATION SITE
The installation site of the instrument should be such that it is
protected from dust, corrosive vapors and vibration. Room temperature
should be constant and lie between 10°C and 35°C. The unit has been
designed to operate in non-condensing atmosphere. Avoid draughts.
Allow 5 cm space on the right hand side of the instrument for sufficient
cooling.
The instrument must be placed on a stable, level surface.
0 1 . 8 7 1 I N S T A L L A T I O N - 4 -

Hi Instruction manual DETECTOR 430 HPLC
1.6 INSTALLATION
1.6.1 Installation of the lamp module
c
c
(1)
(2)
(3)
(4)
Keyboard cover
Locating pins (3 pieces)
Lever for lamp module fixing
Connecting cable
- Pull the.keyboard-cover (1) forewards.
- Insert the lamp module so that the cables exit on the left.
- Ensure that the lamp module is seated properly onto the three locating
pins (2).
- Slide the lever (3) to the right until it snaps in.
- Connect the cables (4).
11.86 1 INSTALLATION - 5 -

Hi Instruction manual DETECTOR 430 HPLC
1.6.2 Installation of the flow cell
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(1) Measuring head
(2) Access holes for mounting screws
( 3 ) L C c e l l
(4 ) Beam splitter block
(5) Mounting screws for LC cell
(6) Mounting pl ate
( 7 ) C a p i l l a r i e s
(8) Guide slot for capillary with clamping nut.
(9) Connector
Proceed as follows:
- Insert the enclosed alien key through the access holes (2) and unscrew
the 2 mounting screws.
- Remove the measuring head (1) by pulling outwards.
- Fit the LC cell (3) tightly onto the beam splitter block (4) and mount
it with the screws (5) using the same alien key.
- The capillaries are placed in the guide slots (8).
- Tighten the guide screw nuts (8) for greater stability during opera
tion.
- Replace the measuring head (1), match the socket with the connector
(9) in the casting and retighten the fixing screws.
IMPORTANT !
For stable operation, always connect the inlet to the capillary going to
the thermoring of the flow cell (see figure in chapter 5.2).
Be sure the cell is mounted before starting the measurements !
08.86 1 INSTALLATION - 6 -

Hi Instruction manual DETECTOR 430 HPLC
1.7 SIGNAL INPUTS AND OUTPUTS
©
NTEGRATOR
1I2I3U
RECOROER
©
COMPUTER
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INPUT RS- 232
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20 pin connector (1): (Watch for the coding, when plugging in.)
Connector pin
location
Integrator 1
2
3
4
Recorder
Computer
Input
Signal description
GN j p j In t eg r a to r o ut p ut 1 : 1 V / AU
Signal
6ND J Integrator output 2 : 1V/AU
GND113 I TTL outPut 3 controls the pen lift:
logic 1:
0: pen up
peri down
Signal )
GND \
Signal )
GND \
Recorder output 1 : lOOmV Full Scale
serial no. 26-1439 and up 1V/FS
Recorder output 2 : lOOmV Full Scale
serial no. 26-1439 and up 1V/FS
Analog output for computer : 1V/AU
Analog output for computer : 2V/AU
Signal
GND
Signal
GND
Signal
GND
^j TTL output 2
TTL 1 and 2 can be used to control an external
A/D converter (see also chapter 3.3.6)
i
j T T L o u t p u t 1
Signal>
> contact closure for event marker
Sienal I contact closure for auto zero
GND
GND
12.89 1 INSTALLATION - 7 -

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Instruction manual DETECTOR 430 HPLC
1.7 SIGNAL INPUTS AND OUTPUTS (cont.)
Via the RS-232C interface (2) the HPLC Detector 430 can be controlled by
an external computer (see chapter 3.3, Remote operation).
The signals on the RS-232C connector are as follows:
5 cond. (180°)
VIEW:
as seen from
connector side
Connector pin Signal
1CTS
2GND
3RXD
4RTS
5TXD
IMPORTANT;
I n o r d e r t o a v o i d i n t e r f e r e n c e w i t h t h e a n a l o g s i g n a l s , u s e o n l y
screened cables which must be as sohrt as possible.
01.87 1 INSTALLATION - 8 -

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H I n s t r u c t i o n m a n u a l d e t e c t o r 4 3 0 u p i Q
TABLE OF CONTENTS
page
2. INSTRUMENT DESCRIPTION
2 . 1 S p e c i fi c a t i o n s 2
2.2 Construction of the instrument 3
2 . 3 K e y b o a r d a n d d i s p l a y 4
2 . 3 . 1 D i s p l a y 4
2 . 3 . 2 F u n c t i o n k e y s 5
0 8 . 8 6 2 I N S T R U M E N T D E S C R I P T I O N - 1 -

18 Instruction manual DETECTOR 430 HPLC
2.1 SPECIFICATIONS
Optics
Monochromator
Wavelength range
Wavelength accuracy
Wavelength precision
Positioning speed
Bandwidth
Photometric Range
Ranges
Double-beam system
concave holographic grating
190 to 800 nm
1 nm
better than 0.05 nm
typically 1000 nm/s
6 nm
-2.000 to +2.000 AÜ
(Absorption Units)
11
Response times 98%
(2, 1, 0.5, 0.2, 0.1, 0.05,
0.02, 0.01, 0.005, 0.002,
0.001 AUFS)
(0.05, 0.1, 0.5, 2, 5 s)
Noise + 2x10 5 AU
(250 nm, empty cell,
resp. time 2s)
Drift after warm up
Str a y l igh t
£ lxlO"4 AU/h
(250 nm, empty cell)
^ 0.08 % (Nal, 220 nm, ASTM E387)
Linearity
Pressure rating of flow cell
•Cell volume
r ä 0.9999
rel. S äs 0.3 %
.(NitroBenzol in Methanol,
254 nm, till 2 AU)
250 bar
3 ul (lu, 0.3ul as options)
—3
Refraction index dependency typically 2x10 AU
Sensiti v i t y t o fl ow
(MeOH — H20, 0-100 %)
-4
less than 5x10 AU
(0-10 ml/min with HO)
11.86 2 INSTRUMENT DESCRIPTION - 2 -

Kl Instruction manual
2.1 SPECIFICATION (cont.)
DETECTOR 430 HPLC
M a t e r i a l s i n c o n t a c t w i t h
the mobile phase
Mains supply
Analog outputs
Remote control
Control signal outputs
Size
Weight
stainless steel (X2CrNiMo 18, 12),
Teflon PFA, Quartz glass
100, 120, 220 and 240 VAC;
50/60 Hz; 150 VA
2 r e c o r d e r 1 0 0 m V / F S
serial no. 26-1439 and up 1V/FS
2 i n t e g r a t o r 1 V / A U
1 c o m p u t e r 1 V / A U
1 c o m p u t e r 2 V / A U
RS-232 interface
Contact closures for Auto Zero and
Event Marker
2 TTL for external data processing.
1 TTL for r eco rd er p en li ft .
WxDxH 170x565x340 mm
25 kg
Hsu«/
12.89 2 INSTRUMENT DESCRIPTION - 3 -

Hi Instruction manual DETECTOR 430 HPLC
2.2 CONSTRUCTION OF INSTRUMENT
Front:
Measuring head
Keyboard and
LED display
Keyboard cover
Mains switch
Rear:
Socket for RS-232
interface cable
20 pin connector
Heat sink
Mains inlet
08.86 2 INSTRUMENT DESCRIPTION - 4 -