LABBOX EASY 40 Xpert User manual

EASY 40 Xpert
Variable volume micropipetter
user manual
Please read the User manual carfully before use,
and follow all operating and safety instructions!

Sr. No. Descripon Page #
1 Introducon 1
1.1 Operang instrucons for using this manual 1
1.2 Danger symbols and danger levels 1
2 Intended use 1
3 Package Contents 1
4 Product descripon 1
4.1 Variable volume pipee range 4
4.2 Fixed volume pipee range 4
4.3 Mul channel pipee range 5
4.4 Seng the volume 5
4.5 Volume indicator display example 6
4.6 Materials 6
5 Pipee operaon 6
5.1 Pipeng guidelines 6
5.2 Seng the volume 7
5.3 Loading ps 8
5.4 Opmum immersion depths 8
5.5 Forward pipeng 8
5.6 Reverse pipeng 9
5.7 Aspiraon of sample 9
5.8 Dispensing sample 9
5.9 Ejecon of ps 9
6 Calibraon and adjustment 9
6.1 Device requirements and test condions 10
6.2 Calibraon adjustment 10
6.3 Procedure to check calibraon 10
6.3.1 Conversion of weight readings to volume 11
6.3.2 Calculaon for in-accuracy (systemac error) 11
6.3.3 Calculaon for imprecision (random error) 11
7 Maintenance and servicing 11
7.1 Disassembly 11
7.2 Disassembling the lower part 11
7.2.1 Disassembling the single channel pipee up to 1ml 12
7.2.2 Disassembling the single channel pipee: 5-10ml 12
7.3 Assembling the pipee 12
7.3.1 Assembling the single channel pipee up to 1ml 12
7.3.2 Assembling the single channel pipee: 5-10ml 12
7.4 Checking the funcon 13
7.5 Disassembling the mul channel pipee 13
7.5.1 Removing the lower assembly 13
7.5.2 Opening the lower assembly 13
7.5.3 Removing the channel 13
7.5.4 Fing the channel 13
7.5.5 Assembling the lower assembly 13
7.5.6 Checking the funcon 13
14
14
7.6
8
Autoclaving
Trouble shooting guide
TABLE OF CONTENTS

1. INTRODUCTION
This manual will guide you on how to take care of your pipee
and benefit the most from its advanced features as follows:
• Magnet assisted piston for precise results
• Innovave spring and seal design for one of the lowest plunge
force requirement
• Corrosion resistant plasc p ejector mechanism with unique
shock absorbing design
• Volume lock
• Fully autoclavable
• Ergonomic design
• Easy in-house calibraon
• Highly durable universal p cone
1.2 DANGER SYMBOLS
The safety instructions in this manual have the following
danger symbols and danger levels:
Material damage
Hazard point
• Read this manual completely before using the device for the
first me.
• This manual is part of the product. Please keep it in an easily
accessible place.
• Enclose this manual when transferring the EASY 40 Xpert
to third parties.
1.1 OPERATING INSTRUCTIONS FOR USING THIS MANUAL
2. INTENDED USE
This pipee is designed and manufactured for dispensing liquids in a
measured way and to be used in combina on with same brand ps
for general laboratory use only.
This pipee is intended exclusively for indoor usage, and for
opera on by trained and skilled personnel.
3. PACKAGE CONTENTS
Descripon Quanty
1
ŸCerficate of conformity including calibraon cerficate 1
1
1
1
1
1
ŸEASY 40 Xpert
ŸWarranty card
ŸProduct manual
ŸShelf-moun ng stand
ŸCalibra on tool
ŸSilicone grease
ŸSample pack of ps 1
4. PRODUCT DESCRIPTION
Fab pipee works on air-displacement principle with the help of
magnet assisted piston for aspira ng and dispensing measured
volume of liquid. It has to be used with disposable ps. The pipee
ps are ejected with the help of ejector.
- 1 -

- 2 -
SINGLE CHANNEL VARIABLE VOLUME
Volume adjustment knob
Volume lock
Tip ejector pusher
Volume range
Coupler
Tip ejector
Tip cone
10
-
100
µl
EASY 40 Xpert

- 3 -
MULTICHANNEL VARIABLE VOLUME
Volume adjustment knob
Volume lock
Tip ejector pusher
Volume range
Coupler
Tip cone with O-ring
Manifold
10
-
100
µl
EASY 40 Xpert

- 4 -
4.1 VARIABLE VOLUME PIPETTE RANGE
0.1-2.5 μl 0.002
0.25
1.25
2.5
12
2.5
2.5
6
1.5
0.7
0.5-10 μl 0.02
1
5
10
2.5
1.5
1
1.5
0.8
0.4
5-50 μl 0.1
5
25
50
2
0.8
0.6
2
0.4
0.3
10-100 μl 0.1
10
50
100
3
1
0.8
1
0.3
0.2
EASD-X02
EASD-X10
EASD-X50
EASD-10X
2-20 μl 0.02
2
10
20
3
1.2
0.9
1.5
0.6
0.3
EASD-X20
Color
Code
Volume
Range
[ μl ]
Incre-
ments
[ μl ]
Test
Volume
[ μl ]
Inac-
curacy
(±) %
Impreci-
sion
(±) %
CAT No.
500-5000 μl 10
500
2500
5000
2.4
1.2
0.6
0.6
0.25
0.2
1000-10000 μl 20
1000
5000
10000
3
0.8
0.6
0.6
0.2
0.15
EASD-5KX
EASD-1XX
20-200 μl 0.2
20
100
200
2.5
0.7
0.6
0.7
0.3
0.2
100-1000 μl 1
100
500
1000
3
1
0.6
0.6
0.2
0.2
EASD-1KX
EASD-20X
30-300 μl 0.2
30
150
300
2.5
1
0.6
0.7
0.3
0.2
EASD-30X

- 5 -
4.3 MULTI-CHANNEL PIPETTE RANGE
8 Channels & 12 Channels
10-100 μl 0.1
10
50
100
3
1
0.8
2
0.8
0.3
EASD-10G
EASD-10T
20-200 μl 0.2
20
100
200
5
1
0.7
1.4
0.4
0.25
EASD-20G
EASD-20T
30-300 μl 0.2
30
150
300
3
1
0.6
1
0.5
0.3
EASD-30G
EASD-30T
0.5-10 μl 0.02
1
5
10
8
4
2
5
2
1
EASD-G10
EASD-T10
2-20 μl 0.02
2
10
20
7
3
2
3
2
1.6
EASD-G20
EASD-T20
5-50 μl 0.1
5
25
50
3
1.5
1
2
1
0.7
EASD-G50
EASD-T50
4.4 SETTING THE VOLUME
Delivery volume is clearly indicated in the
volume display seen in the main body of
the pipee.
In variable volume models, the boom
volume wheel includes a small increment
scale for precise setpoint and delivery
capabili es.
Some variable volume pipees include one
or two decimal places in the setpoint volume
wheels. This is indicated by the use of a black
horizontal line as seen in the following
examples sec on.
Color
Code
Volume
Range
[ μl ]
Incre-
ments
[ μl ]
Test
Volume
[ μl ]
Inac-
curacy
(±) %
Impreci-
sion
(±) %
CAT No.
1
0
0
0

- 6 -
4.6 MATERIALS
NOTICE! Aggressive substances may damage components,
consumables and accessories.
• Check the chemical resistance before using organic solvents or
aggressive chemicals.
• Only use liquids whose vapors do not aack the materials used.
5 PIPETTE OPERATION
5.1 PIPETTING GUIDELINES
• Check the p cone to make sure it is clean.
• While using the pipee make sure that operang plunger is
handled slowly and smoothly.
• Ensure that the p is firmly aached on the p cone. Please
check for foreign parcles and remove if any, around the p
cone.
• Make sure that the temperature of p, pipee and liquid are at
equilibrium.
• While aspirang, hold the pipee in upright posion and keep
the ps at a constant depth below the surface of the liquid.
• Pre-rinse the pipee p before aspirang the sample by
filling and emptying the pipee p five (5) mes. This is
important when dispensing samples that have a viscosity and
density different from water and for volale solvents.
• Do not pre-rinse the p when pipeng samples with
tempe ratures d ifferent fro m the current amb ient
temperature. Be sure to change the pipee p aer each
pipeng.
• For the volale solvents you should saturate the air-cushion of
your pipee by aspirang and dispensing the solvent
repeatedly before aspirang the sample.
• When pipeng liquids with temperature different to the
ambient temperature, pre-rinse ps p several mes before
use.
2.25 μl 18.3 μl10 μl
4.5 VOLUME INDICATOR DISPLAY EXAMPLE
EASD-X02 EASD-X10 EASD-X20
300 μl
EASD-30G
9.30 ml
EASD-1XX
1 ml
EASD-1KX
100 μl
EASD-10X
4.85 ml
EASD-5KX
2
2
5
1
0
0
1
8
3
1
0
0
0
1
0
0
0
3
0
0
0
9
3
4
8
5

- 7 -
• To decrease the volume, turn the
volume seng knob clockwise.
• To increase the volume, turn the
volume seng knob counterclock-
wise.
• Do make sure that the d e s ired
delivery volume is set in-line with
pointer.
• Turn the volume lock to “lock” the
vol um e se ng , p reve nn g any
accidental change in the volume
during pipeng.
For this pipee the delivery volume of
liquid is set using the digital display. A
pointer is u sed to set exact or
intermediate volumes using the scale
on the last wheel of digital display
(refer point # 4.6)
The Locking mechanism ensures that the volume adjustment
knob remains at the setpoint while aspirang or dispensing sample
liquids. Any effort to rotate the volume adjustment knob with the
locking mechanism engaged will damage the locking mechanism
and void the warranty.
5.2 SETTING THE VOLUME
• To set the volume turn the volume lock lever to the “unlock”
posion so that the volume can be adjusted to the desired
setpoint within the permied volume range. Refer following
images
• Aer pipeng acids or other corrosive liquids that emit vapors,
remove the p-cone and rinse the piston, O-ring and seal with
dislled water.
• Do not pipee liquids having temperatures above 70°C. (Not
recommendable)
• Make sure that liquids never enter the p-cone. To prevent
this:
Avoid laying the pipee horizontally when p is filled with
sample. As liquid may enter in and contaminate the sample
during next pipeng cycle.
When there is liquid in the p, press and release the volume
adjustment knob slowly and smoothly.
Never turn the pipee upside down.
• Always store the pipee without p on a shelf mounng
stand supplied with or a pipee carousel stand.
• Highly recommended to calibrate the pipee once in every 3-
6 months (depending on the sensivity of usage) for beer
performance. The calibraon must be carried by gravimetric
method in accordance of DIN ISO 8655-6.
Volume
adjustment
Knob
100
1000
100
1000
µl
100
1000
µl

- 8 -
Tip immersion depth is crical and should not be exceeded, as then
the volume measured may be inaccurate, possibly out of
specificaon. The p angle is also important; the pipee should
always be used in a posion within 20° angle of vercal. See figure
below.
Seng the volume beyond the allowable volume range is not
permied. Using excessive force to turn the volume adjustment
knob outside the permied range will jam the mechanism, damage
the pipee and void the warranty.
5.3 LOADING TIPS
Using correct pipee tip according to the pipee volume range is
recommended. It is necessary to check that the tip cone is clean
before fing a tip. Press the tip on the cone of the pipee firmly
to ensure an air tight seal. Always ensure that the tip is correctly
sealed to avoid any leakage while pipeng.
5.4 OPTIMUM IMMERSION DEPTHS
Volume Immersion depth
0.1 μl - 1 μl 1 mm
1 μl - 100 μl 2 - 3 mm
100 μl - 1000 μl 2 - 4 mm
1ml - 10ml 3 - 5 mm
5.5 FORWARD PIPETTING
1. Press the operang knob unl the first stop.
2. Dip the p under the surface of the liquid in the reservoir and
slowly release the operang knob. Withdraw the p from the
liquid, touching it against the edge of the reservoir to remove
excess liquid.
3. Dispense the liquid by gently pressing
the operang knob to the first stop.
Aer a delay of about one second,
connue to press the operang knob all
the way to the second stop. This acon
will empty the p.
4. Release the operang buon to the
ready posion. Change the p and
connue pipeng.
TIP
ANGLE
IMMERSION DEPTH
TIP
20°