LABBOX EASY 40 Xpert User manual

EASY 40 Xpert
Variable volume micropipetter
user manual
Please read the User manual carfully before use,
and follow all operating and safety instructions!

Sr. No. Descripon Page #
1 Introducon 1
1.1 Operang instrucons for using this manual 1
1.2 Danger symbols and danger levels 1
2 Intended use 1
3 Package Contents 1
4 Product descripon 1
4.1 Variable volume pipee range 4
4.2 Fixed volume pipee range 4
4.3 Mul channel pipee range 5
4.4 Seng the volume 5
4.5 Volume indicator display example 6
4.6 Materials 6
5 Pipee operaon 6
5.1 Pipeng guidelines 6
5.2 Seng the volume 7
5.3 Loading ps 8
5.4 Opmum immersion depths 8
5.5 Forward pipeng 8
5.6 Reverse pipeng 9
5.7 Aspiraon of sample 9
5.8 Dispensing sample 9
5.9 Ejecon of ps 9
6 Calibraon and adjustment 9
6.1 Device requirements and test condions 10
6.2 Calibraon adjustment 10
6.3 Procedure to check calibraon 10
6.3.1 Conversion of weight readings to volume 11
6.3.2 Calculaon for in-accuracy (systemac error) 11
6.3.3 Calculaon for imprecision (random error) 11
7 Maintenance and servicing 11
7.1 Disassembly 11
7.2 Disassembling the lower part 11
7.2.1 Disassembling the single channel pipee up to 1ml 12
7.2.2 Disassembling the single channel pipee: 5-10ml 12
7.3 Assembling the pipee 12
7.3.1 Assembling the single channel pipee up to 1ml 12
7.3.2 Assembling the single channel pipee: 5-10ml 12
7.4 Checking the funcon 13
7.5 Disassembling the mul channel pipee 13
7.5.1 Removing the lower assembly 13
7.5.2 Opening the lower assembly 13
7.5.3 Removing the channel 13
7.5.4 Fing the channel 13
7.5.5 Assembling the lower assembly 13
7.5.6 Checking the funcon 13
14
14
7.6
8
Autoclaving
Trouble shooting guide
TABLE OF CONTENTS

1. INTRODUCTION
This manual will guide you on how to take care of your pipee
and benefit the most from its advanced features as follows:
• Magnet assisted piston for precise results
• Innovave spring and seal design for one of the lowest plunge
force requirement
• Corrosion resistant plasc p ejector mechanism with unique
shock absorbing design
• Volume lock
• Fully autoclavable
• Ergonomic design
• Easy in-house calibraon
• Highly durable universal p cone
1.2 DANGER SYMBOLS
The safety instructions in this manual have the following
danger symbols and danger levels:
Material damage
Hazard point
• Read this manual completely before using the device for the
first me.
• This manual is part of the product. Please keep it in an easily
accessible place.
• Enclose this manual when transferring the EASY 40 Xpert
to third parties.
1.1 OPERATING INSTRUCTIONS FOR USING THIS MANUAL
2. INTENDED USE
This pipee is designed and manufactured for dispensing liquids in a
measured way and to be used in combina on with same brand ps
for general laboratory use only.
This pipee is intended exclusively for indoor usage, and for
opera on by trained and skilled personnel.
3. PACKAGE CONTENTS
Descripon Quanty
1
ŸCerficate of conformity including calibraon cerficate 1
1
1
1
1
1
ŸEASY 40 Xpert
ŸWarranty card
ŸProduct manual
ŸShelf-moun ng stand
ŸCalibra on tool
ŸSilicone grease
ŸSample pack of ps 1
4. PRODUCT DESCRIPTION
Fab pipee works on air-displacement principle with the help of
magnet assisted piston for aspira ng and dispensing measured
volume of liquid. It has to be used with disposable ps. The pipee
ps are ejected with the help of ejector.
- 1 -

- 2 -
SINGLE CHANNEL VARIABLE VOLUME
Volume adjustment knob
Volume lock
Tip ejector pusher
Volume range
Coupler
Tip ejector
Tip cone
10
-
100
µl
EASY 40 Xpert

- 3 -
MULTICHANNEL VARIABLE VOLUME
Volume adjustment knob
Volume lock
Tip ejector pusher
Volume range
Coupler
Tip cone with O-ring
Manifold
10
-
100
µl
EASY 40 Xpert

- 4 -
4.1 VARIABLE VOLUME PIPETTE RANGE
0.1-2.5 μl 0.002
0.25
1.25
2.5
12
2.5
2.5
6
1.5
0.7
0.5-10 μl 0.02
1
5
10
2.5
1.5
1
1.5
0.8
0.4
5-50 μl 0.1
5
25
50
2
0.8
0.6
2
0.4
0.3
10-100 μl 0.1
10
50
100
3
1
0.8
1
0.3
0.2
EASD-X02
EASD-X10
EASD-X50
EASD-10X
2-20 μl 0.02
2
10
20
3
1.2
0.9
1.5
0.6
0.3
EASD-X20
Color
Code
Volume
Range
[ μl ]
Incre-
ments
[ μl ]
Test
Volume
[ μl ]
Inac-
curacy
(±) %
Impreci-
sion
(±) %
CAT No.
500-5000 μl 10
500
2500
5000
2.4
1.2
0.6
0.6
0.25
0.2
1000-10000 μl 20
1000
5000
10000
3
0.8
0.6
0.6
0.2
0.15
EASD-5KX
EASD-1XX
20-200 μl 0.2
20
100
200
2.5
0.7
0.6
0.7
0.3
0.2
100-1000 μl 1
100
500
1000
3
1
0.6
0.6
0.2
0.2
EASD-1KX
EASD-20X
30-300 μl 0.2
30
150
300
2.5
1
0.6
0.7
0.3
0.2
EASD-30X

- 5 -
4.3 MULTI-CHANNEL PIPETTE RANGE
8 Channels & 12 Channels
10-100 μl 0.1
10
50
100
3
1
0.8
2
0.8
0.3
EASD-10G
EASD-10T
20-200 μl 0.2
20
100
200
5
1
0.7
1.4
0.4
0.25
EASD-20G
EASD-20T
30-300 μl 0.2
30
150
300
3
1
0.6
1
0.5
0.3
EASD-30G
EASD-30T
0.5-10 μl 0.02
1
5
10
8
4
2
5
2
1
EASD-G10
EASD-T10
2-20 μl 0.02
2
10
20
7
3
2
3
2
1.6
EASD-G20
EASD-T20
5-50 μl 0.1
5
25
50
3
1.5
1
2
1
0.7
EASD-G50
EASD-T50
4.4 SETTING THE VOLUME
Delivery volume is clearly indicated in the
volume display seen in the main body of
the pipee.
In variable volume models, the boom
volume wheel includes a small increment
scale for precise setpoint and delivery
capabili es.
Some variable volume pipees include one
or two decimal places in the setpoint volume
wheels. This is indicated by the use of a black
horizontal line as seen in the following
examples sec on.
Color
Code
Volume
Range
[ μl ]
Incre-
ments
[ μl ]
Test
Volume
[ μl ]
Inac-
curacy
(±) %
Impreci-
sion
(±) %
CAT No.
1
0
0
0

- 6 -
4.6 MATERIALS
NOTICE! Aggressive substances may damage components,
consumables and accessories.
• Check the chemical resistance before using organic solvents or
aggressive chemicals.
• Only use liquids whose vapors do not aack the materials used.
5 PIPETTE OPERATION
5.1 PIPETTING GUIDELINES
• Check the p cone to make sure it is clean.
• While using the pipee make sure that operang plunger is
handled slowly and smoothly.
• Ensure that the p is firmly aached on the p cone. Please
check for foreign parcles and remove if any, around the p
cone.
• Make sure that the temperature of p, pipee and liquid are at
equilibrium.
• While aspirang, hold the pipee in upright posion and keep
the ps at a constant depth below the surface of the liquid.
• Pre-rinse the pipee p before aspirang the sample by
filling and emptying the pipee p five (5) mes. This is
important when dispensing samples that have a viscosity and
density different from water and for volale solvents.
• Do not pre-rinse the p when pipeng samples with
tempe ratures d ifferent fro m the current amb ient
temperature. Be sure to change the pipee p aer each
pipeng.
• For the volale solvents you should saturate the air-cushion of
your pipee by aspirang and dispensing the solvent
repeatedly before aspirang the sample.
• When pipeng liquids with temperature different to the
ambient temperature, pre-rinse ps p several mes before
use.
2.25 μl 18.3 μl10 μl
4.5 VOLUME INDICATOR DISPLAY EXAMPLE
EASD-X02 EASD-X10 EASD-X20
300 μl
EASD-30G
9.30 ml
EASD-1XX
1 ml
EASD-1KX
100 μl
EASD-10X
4.85 ml
EASD-5KX
2
2
5
1
0
0
1
8
3
1
0
0
0
1
0
0
0
3
0
0
0
9
3
4
8
5

- 7 -
• To decrease the volume, turn the
volume seng knob clockwise.
• To increase the volume, turn the
volume seng knob counterclock-
wise.
• Do make sure that the d e s ired
delivery volume is set in-line with
pointer.
• Turn the volume lock to “lock” the
vol um e se ng , p reve nn g any
accidental change in the volume
during pipeng.
For this pipee the delivery volume of
liquid is set using the digital display. A
pointer is u sed to set exact or
intermediate volumes using the scale
on the last wheel of digital display
(refer point # 4.6)
The Locking mechanism ensures that the volume adjustment
knob remains at the setpoint while aspirang or dispensing sample
liquids. Any effort to rotate the volume adjustment knob with the
locking mechanism engaged will damage the locking mechanism
and void the warranty.
5.2 SETTING THE VOLUME
• To set the volume turn the volume lock lever to the “unlock”
posion so that the volume can be adjusted to the desired
setpoint within the permied volume range. Refer following
images
• Aer pipeng acids or other corrosive liquids that emit vapors,
remove the p-cone and rinse the piston, O-ring and seal with
dislled water.
• Do not pipee liquids having temperatures above 70°C. (Not
recommendable)
• Make sure that liquids never enter the p-cone. To prevent
this:
Avoid laying the pipee horizontally when p is filled with
sample. As liquid may enter in and contaminate the sample
during next pipeng cycle.
When there is liquid in the p, press and release the volume
adjustment knob slowly and smoothly.
Never turn the pipee upside down.
• Always store the pipee without p on a shelf mounng
stand supplied with or a pipee carousel stand.
• Highly recommended to calibrate the pipee once in every 3-
6 months (depending on the sensivity of usage) for beer
performance. The calibraon must be carried by gravimetric
method in accordance of DIN ISO 8655-6.
Volume
adjustment
Knob
100
1000
100
1000
µl
100
1000
µl

- 8 -
Tip immersion depth is crical and should not be exceeded, as then
the volume measured may be inaccurate, possibly out of
specificaon. The p angle is also important; the pipee should
always be used in a posion within 20° angle of vercal. See figure
below.
Seng the volume beyond the allowable volume range is not
permied. Using excessive force to turn the volume adjustment
knob outside the permied range will jam the mechanism, damage
the pipee and void the warranty.
5.3 LOADING TIPS
Using correct pipee tip according to the pipee volume range is
recommended. It is necessary to check that the tip cone is clean
before fing a tip. Press the tip on the cone of the pipee firmly
to ensure an air tight seal. Always ensure that the tip is correctly
sealed to avoid any leakage while pipeng.
5.4 OPTIMUM IMMERSION DEPTHS
Volume Immersion depth
0.1 μl - 1 μl 1 mm
1 μl - 100 μl 2 - 3 mm
100 μl - 1000 μl 2 - 4 mm
1ml - 10ml 3 - 5 mm
5.5 FORWARD PIPETTING
1. Press the operang knob unl the first stop.
2. Dip the p under the surface of the liquid in the reservoir and
slowly release the operang knob. Withdraw the p from the
liquid, touching it against the edge of the reservoir to remove
excess liquid.
3. Dispense the liquid by gently pressing
the operang knob to the first stop.
Aer a delay of about one second,
connue to press the operang knob all
the way to the second stop. This acon
will empty the p.
4. Release the operang buon to the
ready posion. Change the p and
connue pipeng.
TIP
ANGLE
IMMERSION DEPTH
TIP
20°

1. Place the pipee p against the reservoir wall to avoid any
bubbles or splashing of sample out of the reservoir.
2. Press the plunger slowly past the first stroke and second stroke
for complete blow out of liquid sample. For viscous samples, it
is recommended to wait for few seconds ll the fluid passes out
to the reservoir.
3. Pull the p gently along the wall of the reservoir and release the
plunger slowly.
4. Discard the p to avoid any carry over sample or cross
contaminaon. It is highly recommended to change the p and
then repeat the pipeng cycle.
3. Dispense the preset volume of liquid
by gently pressing the operang knob
to the first stop. Hold the operang
knob at the first stop. Some liquid will
remain in the p and it should not be
dispensed.
4. The remaining liquid should either be
discarded with the p or should be
dispensed back into the reagent
reservoir.
1. Press the operang knob unl the second stop.
2. Dip the p under the surface of the liquid in the reservoir and
slowly release the operang knob. This acon will fill the p.
Withdraw the p from the liquid touching it against the edge of
the reservoir to remove excess liquid.
5.6 REVERSE PIPETTING
The reverse pipeng technique is suitable for
dispensing liquids that have high viscosity or a tendency to foam
easily. This technique is also recommended for dispensing very
small volumes.
5.7 ASPIRATION OF SAMPLE
• Hold the pipee vercally; press volume adjustment knob
to its first stop. Place the p into the sample at proper depth
(refer point # 5.4) and relax your thumb pressure on the
plunger. The light piston spring will move the piston upward,
aspirang sample.
• Pause for about 1 second (longer for macro-volume pipees)
to ensure that the full volume of sample is drawn into the p.
• Withdraw the p from the sample. If any liquid remains on
the outer surface of the p, touch it off carefully onto a lint-
free ssue, taking care not touch the tip orifice
5.8 DISPENSING SAMPLE
5.9 EJECTION OF TIPS
The tip ejector needs to be pressed downwards firmly with the
thumb to ensure proper tip ejection. Once the process is
complete, make sure the tips are disposed of into a suitable waste
container.
6 CALIBRATION AND ADJUSTMENT
All pipees hae been quality tested according to ISO8655-6.
The quality control process according to ISO 8655-6 involves
gravimetric tes ng of each pipee with double dis lled water.
All pipees are calibrated in ISO/IEC 17025 accredited laboratory.
Each Pipee is calibrated, inspected and validated by qualified
technicians according to defined quality system.
- 9 -

Depending upon use, we recommend checking of calibraon
every six months. However this can be adjusted to individual
requirements.
6.3 PROCEDURE TO CHECK CALIBRATION
The pipee is checked at maximum volume, at 50 % of maximum
volume and at minimum or 10% of maximum volume, whichever is
higher.
• A new p is first pre-weed 3-5 mes and a series of ten
pipeng is done at each volume.
• Use of forward pipeng technique is recommended.
- 10 -
6.1 DEVICE REQUIREMENTS AND TEST CONDITIONS
An analy cal balance must be used. The balance selec on depends
upon selected model of the pipee and sensi vity of balance
reading.
Test liquid: Water, dis lled or de-ionized, grade 3 water conforming
ISO3696 Calibra on should be carried out in a dra-free room at a
constant (±0.5°C temperature of water, pipee and air between
15°C to 30°C.
The rela ve humidity must be above 50% especially with volumes
under 50 µl, the air humidity should be as high as possible to reduce
the effect of evapora on loss.
Special accessories for analy cal balance, such as the evapora on
trap are recommended for the calibra on of volumes under 50 µl.
6.2 CALIBRATION ADJUSTMENT
1. Calibraon adjustment is done with the calibraon tool
provided along with pipee.
2. Rotate the volume locking lever to the “lock” posion so the
volume seng mechanism is locked and able to turn the
calibraon screw.
3. Remove volume adjustment knob by pulling it upwards.
4. Place the calibraon tool into the calibraon grooves.
5. Rotate the calibraon tool counter clockwise to increase and
clockwise to decrease the volume.
6. Aer adjustment, check the calibraon according to the
instrucon in point number 6.3
7. Once within permissible error range, remove the calibraon
tool from pipee and place volume adjustment knob in its
original posion.
100
1000
µl

7 MAINTENANCE & SERVICING
When the pipee is not in use it should be stored in an
upright posi on. The pipee should be inspected prior to use each
day for any dust or contamina on on outside surfaces. Special
aen on should be given to the p cone. No solvent other than
isopropanol should be used to clean the pipee. If the pipee is
used daily, an internal parts inspection should be performed
every three (3) months.
7.1 DISASSEMBLY
The servicing procedure starts with the disassembly of the pipee.
Please refer to the spare parts lists for beer understanding of the
components.
7.2 DISASSEMBLING THE LOWER PART
1. Press p ejector pusher completely down and hold.
2. Pull down the p ejector and release the p ejector pusher.
- 11 -
• Calculate the inaccuracy and imprecision for all three
volumes as per EN ISO 8655-6 standards on the basis of the
following calculaon
6.3.1 CONVERSION OF WEIGHT READINGS TO VOLUME
Mean Volume
Mean Weight
Balance Reading
number of reading
Conversion factor
[example Z=1.0040 µl/mg at 25°C and 1013 hPa]
Mean Value
Particular volume at which readings are taken
S = Standard Deviation
n = number of readings
S= V = Mean Value
i=1
n
∑ ( Vi - V)2
n - 1
CV% = 100 · S
_
V
Compare the results to the
limits in the earlier tables (Page )# 4, 5
6.3.2 CALCULATION FOR IN-ACCURACY (SYSTEMATIC ERROR)
6.3.3 CALCULATION FOR IMPRECISION (RANDOM ERROR)

7.2.2 DISASSEMBLING THE SINGLE-CHANNEL PIPETTE: 5 - 10ml
1. As per earlier process remove the
ejector by pulling it aer pressing
the p ejector pusher completely down.
2. Unscrew the coupler to p cone and
pull out the piston and other parts from
p cone.
- Remember to keep all parts in order for
reassembly.
- Clean the piston, the piston spring, seal
and the o-ring with isopropanol and lint
free ssue. Allow them to dry.
- Check the p cone for foreign parcles
and remove, if any. Grease the cleaned
parts with the approved lubricant
provided with each pipee.
- 12 -
1. Unscrew the coupler and remove p
cone.
2. Remove the lower part and pull out
the piston and other parts from
p cone.
- Remember to keep all parts in order
for reassembly.
- Clean the piston, the piston spring,
seal and the o-ring with isopropanol
and lint free ssue. Allow them to
dry.
- Check the  p cone for fo reign
arcles and remove, if any. Grease the
cleaned parts with the approved
lubricant provided with each pipee.
7.2.1 DISASSEMBLING THE SINGLE-CHANNEL PIPETTE UP TO 1ml
1. Carefully insert the piston into the p cone.
2. Press on piston from above and check for free movement.
The piston must be able to move freely without resistance.
3. Reconnect the p cone to the main body by screwing into the
threaded secon.
4. Reinstall the p ejecon collar.
7.3 ASSEMBLING THE PIPETTE
7.3.1 ASSEMBLING THE SINGLE-CHANNEL PIPETTE UP TO 1ml
7.3.2 ASSEMBLING THE SINGLE-CHANNEL PIPETTE: 5 - 10ml
1. Insert the seal on posion.
2. Keep retainer on piston and spring. Press the spring to fit
with piston.
3. Carefully insert the piston into the p cone.
4. Press on piston from above and check for free movement.
The piston must be able to move freely without resistance.
5. The coupler screw to p cone and screw with body.
6. Fit the ejector collar.

1. Press the p ejector pusher completely
and hold it while unscrewing the coupler
from upper part of pipee.
2. Remove the lower assembly.
7.5.2 OPENING THE L7.5.2 OPENING THE LOOWER ASSEMBLWER ASSEMBLYY
1. Unscrew the two small screw from back side and safely keep it.
(Refer image -1)
2. Press and push up from side to open the front cover.
(Refer image -2)
- 13 -
7.4 CHECKING THE FUNCTION
It is to ensure that the pipee has been assembled correctly.
- Carry out a gravimetric test for the systemac and random
error.
7.5 DISASSEMBLING THE MULTI-CHANNEL PIPETTE
7.5.1 REMOVING THE LOWER ASSEMBLY
1. Slightly push the spring and pull up p cone to remove it from
the lower rail. (image -3)
2. Carefully release the piston from the upper rail and move
upward to remove it. (image -4)
7.5.3 REMOVING THE CHANNEL
1. Insert the spring with cylinder into the centre rail.
2. Insert the piston into the cylinder and fit into upper rail.
3. Compress the spring with the cylinder and insert the cylinder
into the lower rail.
7.5.5 ASSEMBLING THE LOWER ASSEMBLY
1. Aach the front cover and screw it.
2. Press the pusher completely and hold it while screwing the
coupler nut to body.
7.5.6 CHECKING THE FUNCTION
It is to ensure that the pipee has been assembled correctly.
- Carry out a gravimetric test for the systemac and random
error.
image -1 image -2
7.5.4 FITTING THE CHANNEL
image -4image -3
Move
Upward
Push

7.6 AUTOCLAVING
Fab pipee is completely autoclavable at 1 bar pressure and 121°
C temperature for 20 minutes exposure me.
AUTOCLAVING INSTRUCTIONS
ŸKeep digital counter in unlock posion.
ŸDo not dis-assemble the pipee for autoclaving.
ŸAer autoclaving, allow the pipee to completely cool and fully
dry for a minimum of four (4) hours.
Pipette is leaking
Pipette does not
aspirate the
solution
Pipette is
inaccurate
Inaccurate
dispensing with
certain liquids
Worn o-ring or seal
Worn o-ring or seal
Tip cone is loose
Damaged tip cone
Improper assembly
Tip incorrectly attached
Calibration altered
Calibration not suitable for
particular liquid
Recalibrate with the
liquid in question
Attach firmly
Recalibrate according to
instructions
See “Maintenance”
section
Replace the tip cone
Piston is damaged
(Chemically or mechanically)
Return pipette to
authorised distributor
Tighten tip cone
Foreign particles between tip
and tip cone
Foreign particles between
piston and seal
Clean tip cone, attach
new tip
Replace worn parts
Replace worn parts
Clean seal and piston
Problem area Possible Cause Solution
Tip cone is loose Tighten tip cone
- 14 -
If the pipee is autoclaved frequently, the piston and springs should
be greased with supplied lubricant along with each pipee to
maintain smooth movement.
8 TROUBLE SHOOTING GUIDE