LaMotte DC1500 PHOSPHATE User manual

DC1500 PHOSPHATE
Phosphate Colorimeter Kit
Code 3242
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CONTENTS
DC1500 Phosphate Kit
Kit Contents.......................................................................................... 5
Accessories .......................................................................................... 5
Test Method Specifications
Introduction.......................................................................................... 6
Application............................................................................................ 6
Range..................................................................................................... 6
Method .................................................................................................. 6
Handling & Preservation .................................................................... 6
Interferences........................................................................................ 6
Analysis
Phosphate............................................................................................. 7
Calibration
Standards ............................................................................................. 9
Calibration Procedure......................................................................... 9
Set Up
Setting the Clock ...............................................................................13
Logging Data ......................................................................................14
Factory Setup.....................................................................................15
Setting Power Save ...........................................................................15
Setting Backlight Time .....................................................................16
Selecting A Language .......................................................................17
Looping Menus...................................................................................18
Computer Connection
Output..................................................................................................19
Computer Connection.......................................................................19
WaterLink Connect.............................................................................19
Battery
Battery/AC Operation........................................................................19
Battery Replacement........................................................................20
Maintenance
Cleaning ..............................................................................................20
Repairs ................................................................................................20
Meter Disposal ...................................................................................21
General Operating Information
Overview..............................................................................................21
General Operating Information .......................................................21
The Keypad .........................................................................................22
The Display and Menus .....................................................................22
Tubes and Chambers.........................................................................24
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Sample Dilution Techniques ............................................................25
General information
Packaging and Delivery ....................................................................25
General Precautions..........................................................................25
Safety Precautions............................................................................26
Limits of Liability ...............................................................................26
Specifications ....................................................................................27
CE .........................................................................................................28
IP67......................................................................................................28
Warranty .............................................................................................28
Register Your Meter...........................................................................28
Trouble Shooting Guide
Error Messages ..................................................................................29
Calibration ..........................................................................................29
Stray Light ..........................................................................................29
Troubleshooting Guide......................................................................30
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DC1500 PHOSPHATE KIT
Kit Contents
QUANTITY CONTENTS CODE
2 x 60 mL *Phosphate Acid Reagent V-6282-H
10 g *Phosphate Reducing Reagent *V-6283-D
1 Spoon, 0.1 g, plastic 0699
1 Water Sample Collecting Bottle 0688
1 Pipet, 1.0 mL, plastic 0354
1 Colorimeter Tubes, w/caps, set of 6 0290-6
1 1500 Colorimeter for Phosphate 27926-PH
1 USB Wall Adapter 1721
1 USB Cable 1720-01
1 DC1500-PH Phosphate Colorimeter, Manual 3242-MN
1 DC1500-PH Phosphate Colorimeter,
Quick Start Guide
3242-QG
To order individual reagents or test kit components, use the specifiedcode
number.
Accessories
DESCRIPTION CODE
Test Tubes, with Caps 0290-6
Replacement Chamber 3-0038
USB Cable 1720-01
USB Wall Adapter 1721
Car Charger 5-0132
WARNING: Only use the USB cable (code 1720-01) that is supplied with the kit.
Make no substitutions.
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TEST METHODS SPECIFICATIONS
INTRODUCTION
Phosphorus is an important nutrient for aquatic plants. The amount found
in water is generally not more than 0.1 ppm unless the water has become
polluted from waste water sources or excessive drainage from agricultural
areas. When phosphorus is present in excess of the concentrations required
for normal aquatic plant growth, a process called eutrophication takes place.
This creates a favorable environment for an increase in algae and weed
nuisances. When algae cells die, oxygen is used in the decomposition and fish
kills often result. Rapid decomposition of dense algae scums with associated
organisms give rise to foul odors and hydrogen sulfide gas.
APPLICATION
Drinking, surface and saline waters; domestic and industrial wastes (Method
based on reactions that are specific for orthophosphate).
RANGE
0 to 3.0 ppm Orthophosphate (Range can be extended by dilution.)
METHOD
Ammonium molybdate and antimony potassium tartrate react in a
filtered acid medium with dilute solution of PO43- to form an antimony-
phosphomolybdate complex. This complex is reduced to an intense blue
colored complex by ascorbic acid. The color is proportional to the amount
of phosphate present. (Only orthophosphate forms a blue color in this
test.) Polyphosphates (and some organic phosphorus compounds) may be
converted to the orthophosphate form by sulfuric acid digestion. Organic
phosphorus compounds may be converted to the orthophosphate form by
persulfate digestion.
HANDLING & PRESERVATION
If benthic deposits are present in the area being sampled, great care should
be taken not to include these deposits. If the analysis cannot be performed
the same day of collection, the sample should be preserved by the addition
of 2 mL of concentrated sulfuric acid or 40 mg mercuric chloride per liter and
refrigerated at 4°C.
INTERFERENCES
1. No interference from copper, iron, or silicate at concentrations many
times the
concentration of sea water. However, high iron concentrations can
cause precipitation
and subsequent loss of phosphorus.
2. Salt error for samples ranging from 5% to 20% salt content was found to
be less than 1%.
3. Mercuric chloride, HgCl2, when used as the preservative, interferes
when the chloride levels are low (less than 50mg/L). This interference
is overcome by spiking samples with a minimum of 50 mg/L of sodium
chloride.
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ANALYSIS
PROCEDURE
1. Fill the Water Sample Collecting
Bottle (0688) with sample
water.
2. Press and hold until
colorimeter turns on.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
3. Press
ENTER to select Testing
Menu.
All Tests
081 Phosphate LR
12:00:00 001/500
4. Scroll to and select 081
Phosphate LR from menu.
081 Phosphate LR
Scan Blank
Scan Sample
12:00:00 001/500
5. Rinse a clean tube (0290) with
sample water. Fill to the 10 mL
line with sample. Cap and wipe
dry.
Analysis
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6. Insert tube into chamber, close
lid and select Scan Blank.
7. Remove tube from colorimeter.
Use 1.0 mL pipet (0354) to
add 1.0 mL of *Phosphate Acid
Reagent (V-6282). Cap and mix.
8. Use the 1.0 g spoon (0699) to
add one measure of *Phosphate
Reducing Reagent (V-6283).
9. Cap and shake until powder
dissolves. Wait 5 minutes for
full color development. Solution
will turn blue if phosphates are
present. Wip tube dry.
05:00
10. Insert tube into chamber. Close
lid. Select Scan Sample. Record
result as Ammonia-nitrogen.
081 Phosphate LR
0.99 ppm
Scan BLank
Scan Sample
12:00:00 001/500
NOTE: The meter will remember the last scanned blank reading. It is not
necessary to scan a blank each time the test is performed. To use the
previous blank reading, instead of scanning a new one, scroll to Scan Sample
and proceed. For the most accurate results, the meter should be blanked
Analysis
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before each test and the same tube should be used for the blank and the
reacted sample.
NOTE: For the best possible results, carry a reagent blank through the
procedure.
After scanning the blank, perform the test procedure on clear,
colorless,
distilled or deioized water. Subtract results of regent blank from all
subsequent test results.
CALIBRATION
STANDARDS
The meter should be calibrated with ortho phosphate standards. The
calibration should be done with a distilled or deionized water blank and
one phosphate standard of known concentration. The concentration of the
calibration standard should be similar to the expected concentration of the
sample that will be tested.
CALIBRATION PROCEDURE
1. Press and briefly hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
2. Press
ENTER to select Testing
Menu.
All Tests
081 Phosphate LR
12:00:00 001/500
3. Scroll to and select 081
Phosphate LR from menu.
081 Phosphate LR
Scan Blank
Scan Sample
12:00:00 001/500
Calibration
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4. Rinse a clean tube (0290)
three times with the phosphate
standard. Fill to the 10 mL line
with the phosphate standard.
Cap the tube. Dry the tube with a
lint-free cloth.
5. Insert tube into chamber, close
lid and select Scan Blank.
6. Remove tube from colorimeter.
Use 1.0 mL pipet (0354) to
add 1.0 mL of *Phosphate Acid
Reagent (V-6282). Cap and mix.
7. Use the 1.0 g spoon (0699) to
add one measure of *Phosphate
Reducing Reagent (V-6283).
8. Cap and shake until powder
dissolves. Wait 5 minutes for
full color development. Solution
will turn blue if phosphates are
present. Wip tube dry.
05:00
Calibration
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9. Insert tube into chamber. Close
lid. Select Scan Sample.
081 Phosphate LR
0.99 ppm
Scan BLank
Scan Sample
12:00:00 001/500
10. Press to scroll to
Calibrate.
081 Phosphate LR
0.99 ppm
%T/Abs
Calibrate
12:00:00 001/500
11. Press ENTER to select Calibrate.
A reverse font (light background
with dark characters) will appear
to indicate that the reading can
be adjusted.
081 Phosphate LR
0.99 ppm
, =Edit, ENTER=Save
+ENTER=Default
12:00:00 001/500
12. Press or to
adjust the value shown to the
concentration of the prepared
standard, 1.00 in this example.
NOTE: A maximum adjustment
of 25% is possible.
081 Phosphate LR
1.00 ppm
, =Edit, ENTER=Save
+ENTER=Default
12:00:00 001/500
Calibration
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