LaMotte DC1500 UREA User manual

DC1500 UREA
Urea Colorimeter Kit
Code 3684-01

2

CONTENTS
DC1500 Urea Kit
Kit Contents...............................................................................................4
Accessories................................................................................................5
Test Method Specifications
Introduction...............................................................................................5
Application.................................................................................................5
Range..........................................................................................................5
Method........................................................................................................5
Handling & Preservation.........................................................................5
Interferences.............................................................................................5
Analysis
Urea.............................................................................................................6
Calibration
Standards ............................................................................................... 10
Calibration Procedure........................................................................... 10
Set Up
Setting the Clock ................................................................................... 15
Logging Data .......................................................................................... 16
Factory Setup......................................................................................... 17
Setting Power Save............................................................................... 17
Setting Backlight Time......................................................................... 18
Selecting A Language........................................................................... 19
Looping Menus....................................................................................... 20
Computer Connection
Output...................................................................................................... 21
Computer Connection .......................................................................... 21
Battery
Battery/AC Operation............................................................................ 21
Battery Replacement............................................................................ 22
Maintenance
Cleaning................................................................................................... 22
Returns.................................................................................................... 22
Meter Disposal ....................................................................................... 23
General Operating Information
Overview.................................................................................................. 23
General Operating Information........................................................... 23
The Keypad ............................................................................................. 24
The Display and Menus......................................................................... 24
Tubes and Chambers ............................................................................ 26
Sample Dilution Techniques................................................................ 27

4
General information
Packaging and Delivery........................................................................ 27
General Precautions ............................................................................. 27
Safety Precautions................................................................................ 28
Limits of Liability ................................................................................... 28
Specifications ........................................................................................ 29
CE.............................................................................................................. 30
IP67.......................................................................................................... 30
Warranty.................................................................................................. 30
Trouble Shooting Guide
Error Messages ...................................................................................... 31
Calibration .............................................................................................. 31
Stray Light .............................................................................................. 31
Troubleshooting Guide ......................................................................... 32

5
DC1500 UREA KIT
Kit Contents
QUANTITY CONTENTS CODE
25 Urease Delivery Strips 2939-G
60 mL *Salicylate Ammonia #1 *3978-H
10 g *Salicylate Ammonia #2 *7457-D
10 g *Salicylate #3 Reagent Powder *7458-D
1Spoon, 0.1 g, plastic 0699
1Spoon, 0.15 g, plastic 0727
1Pipet, 1 mL Plastic 0354
1Colorimeter Tubes, w/caps,set of 6 0290-6
1Water Sample Collecting Bottle 0688
11500 Colorimeter for Urea 27926-AM
1USB Wall Adapter 1721
1USB Cable 1720-01
1DC1500-UR Urea Colorimeter, Manual 3684-01-MN
1DC1500-UR Urea Colorimeter,
Quick Start Guide
3684-01-QG
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to
www.lamotte.com. Search the four digit reagent code number listed on the
reagent label, in the contents list or in the test procedures. Omit any letter that
follows or precedes the four digit code number. For example if code is 4450WT-
H, search 4450. To obtain a printed copy, contact LaMotte by e-mail, phone or
fax.
Emergency information for all LaMotte reagents is available from Chem-Tel (US,
1-800-255-3924) (International, call collect, 813-248-0585).
NOTE: Store Urease Delivery Strips at or below room temperature (21 °C,
70 °F).
To order individual reagents or test kit components, use the specified code
number.

6
Accessories
DESCRIPTION CODE
Test Tubes, with Caps 0290-6
Replacement Chamber 3-0038
USB Cable 1720-01
USB Wall Adapter 1721
Car Charger 5-0132
WARNING: Only use the USB Cable (1720-01) that is supplied with the kit. Make
no substitutions.
TEST METHODS SPECIFICATIONS
INTRODUCTION
Urea is the main nitrogen-containing contaminant in swimming pools. It reacts
with free available chlorine to form chloramines which can cause taste and odor
problems and eye irritation. Urea also acts as a nutrient for algae and bacteria.
It is introduced by bather’s sweat and is the main component of urine.
APPLICATION
Swimming Pools
RANGE
0.0 – 6.0 mg/L urea
METHOD
Urease catalyzes the hydrolysis of urea to produce ammonia. Salicylate
and ammonia react at high pH in the presence of a chlorine donor and an
iron catalyst to form a blue indophenol dye, the concentration of which is
proportional to the urea concentration in the sample.
HANDLING & PRESERVATION
Urea solutions are unstable and should be analyzed as soon as possible.
INTERFERENCES
High concentrations of reducing agents will react with the chlorine donor and
result in a negative interference. Color and turbidity can also interfere.

7
ANALYSIS
PROCEDURE
1. Fill the Water Sample Collecting
Bottle (0688) with sample water.
2. Press and hold until
colorimeter turns on.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
3. Press ENTER to select Testing
Menu.
All Tests
096 Urea
12:00:00 001/500
4. Scroll to and select 096 Urea
from menu.
096 Urea
Scan Blank
Scan Sample
12:00:00 001/500
5. Rinse a clean tube (0290) with
sample water. Fill to the 10 mL
line with sample. Cap and wipe
dry.
Analysis

8
6. Insert tube into chamber, close
lid and select Scan Blank.
7. Remove tube from colorimeter.
Submerge a Urease Delivery
Strip (2939) in the sample.
Plunge the strip up and down for
30 seconds. (Avoid hitting the
bottom of the tube.) Remove and
discard strip.
8. Wait exactly 5 minutes.
05:00
9. At the end of 5 minute waiting
period, use the 1.0 mL plastic
pipet (0354) to add 2.0 mL of
*Salicylate Ammonia #1 (3978).
(Fill pipet to the line for accurate
results.)
10. Cap tube. Invert 2 times to mix.
(Do not shake vigorously.)
Analysis

9
11. Use the 0.15 g spoon (0727)
to add two level measures of
*Salicylate #2 Reagent (7457).
(To add a level spoon of powder,
tap spoon on edge of reagent
container to remove excess
powder. Do not add excess
powder.)
12. Cap tube. Invert to mix for
30 seconds or until powder
dissolves. (Do not shake
vigorously.)
13. Wait exactly one minute.
01:00
14. At the end of one minute
waiting period use the 0.1 g
spoon (0699) to add two level
measures of *Salicylate #3
Reagent (7458). (To add a level
spoon of powder, tap spoon on
edge of reagent container to
remove excess powder. Do not
add excess powder.)
15. Cap and shake vigorously for at
least 30 seconds until all solid
has dissolved.
16. Wait exactly 12 minutes for
maximum color development.
12:00
Analysis

10
17. At the end of the 12 minute
waiting period, invert once to
mix. Immediately insert tube into
chamber. Close lid. Select Scan
Sample. Record result as Urea.
096 Urea
3.3 mg/L
Scan Blank
Scan Sample
12:00:00 001/500
NOTE: The meter will remember the last scanned blank reading. It is not
necessary to scan a blank each time the test is performed. To use the previous
blank reading, instead of scanning a new one, scroll to Scan Sample and
proceed. For the most accurate results, the meter should be blanked before
each test and the same tube should be used for the blank and the reacted
sample.
NOTE: For the best possible results, carry a reagent blank through the
procedure. After scanning the blank, perform the test procedure on clear,
colorless, distilled or deioized water. Subtract results of regent blank from all
subsequent test results.
Analysis

11
CALIBRATION
STANDARDS
The meter should be calibrated with urea standards. The calibration should be
done with a distilled or deionized water blank and one urea standard of known
concentration. The concentration of the calibration standard should be similar
to the expected concentration of the sample that will be tested.
CALIBRATION PROCEDURE
1. Press and briefly hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
2. Press ENTER to select Testing
Menu.
All Tests
096 Urea
12:00:00 001/500
3. Scroll to and select 096 Urea
from menu.
096 Urea
Scan Blank
Scan Sample
12:00:00 001/500
4. Rinse a clean tube (0290) three
times with the urea standard. Fill
to the 10 mL line with the urea
standard. Cap the tube. Dry the
tube with a lint-free cloth.
Calibration

12
5. Insert tube into chamber, close
lid and select Scan Blank.
6. Remove tube from colorimeter.
Submerge a Urease Delivery
Strip (2939) in the standard.
Plunge the strip up and down for
30 seconds. (Avoid hitting the
bottom of the tube.) Remove and
discard strip.
7. Wait exactly 5 minutes.
05:00
8. At the end of 5 minute waiting
period, use the 1.0 mL plastic
pipet (0354) to add 2.0 mL of
*Salicylate Ammonia #1 (3978).
(Fill pipet to the line for accurate
results.)
9. Cap tube. Invert 2 times to mix.
(Do not shake vigorously.)
Calibration

13
10. Use the 0.15 g spoon (0727)
to add two level measures of
*Salicylate #2 Reagent (7457).
(To add a level spoon of powder,
tap spoon on edge of reagent
container to remove excess
powder. Do not add excess
powder.)
11. Cap tube. Invert to mix for
30 seconds or until powder
dissolves. (Do not shake
vigorously.)
12. Wait exactly one minute.
01:00
13. At the end of one minute
waiting period use the 0.1 g
spoon (0699) to add two level
measures of *Salicylate #3
Reagent (7458). (To add a level
spoon of powder, tap spoon on
edge of reagent container to
remove excess powder. Do not
add excess powder.)
14. Cap and shake vigorously for at
least 30 seconds until all solid
has dissolved.
Calibration

14
15. Wait exactly 12 minutes for
maximum color development.
12:00
16. At the end of the 12 minutes
waiting period, invert once to
mix. Immediately insert tube into
chamber. Close lid. Select Scan
Sample. Record results as Urea.
096 Urea
0.99 mg/L
Scan BLank
Scan Sample
12:00:00 001/500
17. Press to scroll to
Calibrate.
096 Urea
0.99 mg/L
%T/Abs
Calibrate
12:00:00 001/500
18. Press ENTER to select Calibrate.
A reverse font (light background
with dark characters) will appear
to indicate that the reading can
be adjusted.
096 Urea
0.99 mg/L
∧, ∨=Edit, ENTER=Save
∧ +ENTER=Default
12:00:00 001/500
19. Press or to
adjust the value shown to the
concentration of the prepared
standard, 1.00 in this example.
NOTE: A maximum adjustment of
25% is possible.
096 Urea
1.00 mg/L
∧, ∨=Edit, ENTER=Save
∧ +ENTER=Default
12:00:00 001/500
Calibration

15
20. Press ENTER to save the value.
To leave the Calibration
procedure without saving the
adjustment, press EXIT .
Press and ENTER at any
time to return to the default
value.
The calibration has now been
standardized and can be used for
testing. Scroll to Scan Blank and
begin testing.
096 Urea
1.00 mg/L
%T/Abs
Calibrate
12:00:00 001/500
Calibration

16
SET UP
SETTING THE CLOCK
Setting the clock allows the correct time and date stamp to be stored with each
reading in the data logger.
1. From the Editing Menu, press
or to scroll to Set
Clock.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
2. Press ENTER to select Set Clock.
The year is displayed. Press
or to scroll to the
appropriate character. Press
ENTER to select the character.
The month, day, hour, format
hour, minute, second, AM/PM will
be dislpayed. Repeat for each.
Set Time
Year: 2000
12:00:00 001/500
3. Press ENTER to select the final
character. The time and date
will be saved and the meter will
return to the Editing Menu.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500

17
LOGGING DATA
The default setting for the data logger is enabled. The meter will log the last 500
data points. The counter in the center bottom of the display will show how many
data points have been logged. The display will show 500+ when the data logger
has exceeded 500 points and the data points are being overwritten.
1. From the Editing Menu, press
or to scroll to
Logging.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
2. Press ENTER to select Logging. Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
3. Press or to scroll to
desired function.
Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
4. Press ENTER . The screen will
display Storing... for about 1
second and return to the Editing
Menu.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500

18
FACTORY SETUP
The Factory Setup menu is used in manufacturing of the colorimeter. This menu
is not for use by the operator in the field.
SETTING POWER SAVE
The power saving Auto Shutoff feature will turn the meter off when a button has
not been pushed for a set amount of time. The default setting is disabled. To
change the setting:
1. From the Editing Menu, press
or to scroll to Set
Power Save.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
2. Press ENTER to select Set Power
Save.
Set Power Save
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
1. Press or to scroll to
desired function.
Set Power Save
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
2. Press ENTER . The screen will
display Storing.... for about 1
second and the meter will retun
to the Editing Menu.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500

19
SETTING THE BACKLIGHT TIME
The backlight illuminates the display for enhanced viewing. The default setting
is 10 seconds. If Button Control is chosen the backlight button on the key pad
will act as an on/off switch and the backlight will remain on or off when the
meter is being used. When one of the other settings – 10, 20 or 30 seconds – is
chosen, the display will be illuminated for the specified amount of time after any
button is pressed.
NOTE: The backlight feature uses a significant amount of power. The longer the
backlight is on, the more frequently the battery will have to be charged if the
USB/Wall Adapter is not being used.
1. From the Editing Menu, press
or to scroll to
Backlight Time.
Editing Menu
Logging
Factory Setup
Set Power Save
Set Backlight Time
12:00:00 001/500
2. Press ENTER to select Set
Backlight Time.
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
3. Press or to scroll to
desired option.
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
4. Press ENTER . The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
Editing Menu
Logging
Factory Setup
Set Power Save
Set Backlight Time
12:00:00 001/500

20
SELECTING A LANGUAGE
There are seven languages available: English, Spanish, French, Portuguese,
Italian, Chinese, and Japanese.
1. From the Editing Menu, press
or to scroll to
Select Language.
Editing Menu
Factory Setup
Set Power Save
Set Backlight Time
Select Language
12:00:00 001/500
2. Press ENTER to select Select
Language.
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
3. Press or to scroll to
desired language.
Select Language
English
Spanish
French
Portugese
12:00:00 001/500
4. Press ENTER . The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
Editing Menu
Set Power Save
Set Backlight Time
Bluetooth Menu
Select Language
12:00:00 001/500
NOTE: If meter unintentionally switches to another language, use the procedure
above to reset the meter to the desired language. For example, to reset the
meter to English:
Turn meter on.
1. Press one time. Press ENTER .
2. Press five times. Press ENTER .
1. Press ENTER .