LaMotte DC1500 AMMONIA User manual

DC1500 AMMONIA
Ammonia Colorimeter Kit
Code 3241

CONTENTS
DC1500 Ammonia Nitrogen Kit
Kit Contents.................................................................................................................................................................3
Accessories .................................................................................................................................................................3
Test Method Specifications
Introduction.................................................................................................................................................................3
Application...................................................................................................................................................................3
Range.............................................................................................................................................................................3
Method..........................................................................................................................................................................3
Handling & Preservation.........................................................................................................................................3
Interferences...............................................................................................................................................................4
Analysis
Ammonia Nitrogen....................................................................................................................................................4
Calibration
Standards.....................................................................................................................................................................6
Calibration Procedure..............................................................................................................................................6
Set Up
Setting the Clock .......................................................................................................................................................9
Logging Data...............................................................................................................................................................9
Factory Setup ..........................................................................................................................................................10
Setting Power Save................................................................................................................................................ 10
Setting Backlight Time ......................................................................................................................................... 11
Selecting A Language ........................................................................................................................................... 12
Looping Menus........................................................................................................................................................ 12
Computer Connection
Output.........................................................................................................................................................................13
Computer Connection...........................................................................................................................................13
Battery
Battery/AC Operation ............................................................................................................................................ 13
Battery Replacement............................................................................................................................................ 14
Maintenance
Cleaning..................................................................................................................................................................... 14
Returns ......................................................................................................................................................................14
Meter Disposal......................................................................................................................................................... 14
General Operating Information
Overview..................................................................................................................................................................... 15
General Operating Information.......................................................................................................................... 15
The Keypad ............................................................................................................................................................... 15
The Display and Menus......................................................................................................................................... 15
Tubes and Chambers ............................................................................................................................................17
Sample Dilution Techniques ............................................................................................................................... 17
General Information
Packaging and Delivery ........................................................................................................................................17
General Precautions.............................................................................................................................................. 17
Safety Precautions.................................................................................................................................................18
Limits of Liability..................................................................................................................................................... 18
Specifications.......................................................................................................................................................... 18
CE.................................................................................................................................................................................19
IP67............................................................................................................................................................................. 19
Warranty....................................................................................................................................................................19
Troubleshooting Guide
Error Messages ....................................................................................................................................................... 19
Calibration................................................................................................................................................................. 19
Stray Light................................................................................................................................................................. 19
Troubleshooting Guide.......................................................................................................................................... 20

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DC1500 AMMONIA NITROGEN KIT
KIT CONTENTS
QUANTITY CONTENTS CODE
30 mL Ammonia Nitrogen Reagent #1 V-4797-G
3 x 30 mL *Ammonia Nitrogen Reagent #2 *V-4798-G
1Pipet, 1 mL Plastic 0354
1Colorimeter Tubes, w/caps,set of 6 0290-6
1Water Sample Collecting Bottle 0688
11500 Colorimeter for Ammonia Nitrogen 27926-AM
1USB Wall Adapter 1721
1USB Cable 1720-01
1DC1500-AM Ammonia Colorimeter, Manual 3241-MN
1DC1500-AM Ammonia Colorimeter, Quick Start Guide 3241-QG
*Reagent is a potential health hazard. READ SDS:
lamotte.com. Emergency information:
Chem-Tel USA 1-800-255-3924
Int’l, call collect, 813-248-0585
To order individual reagents or test kit components, use the specified code number.
ACCESSORIES
DESCRIPTION CODE WARNING: Only use the USB cable (1720-01) that is
supplied with the kit. Make no substitutions.
Test Tubes, with Caps 0290-6
Replacement Chamber 3-0038
USB Cable 1720-01
USB Wall Adapter 1721
Car Charger 5-0132
TEST METHODS SPECIFICATIONS
INTRODUCTION
Ammonia nitrogen is present in various concentrations in many surface and ground water
supplies. Any sudden change in the concentration of ammonia nitrogen in a water supply
is cause for suspicion. A product of microbiological activity, ammonia nitrogen is sometimes
accepted as chemical evidence of pollution when encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial groups that
produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in
the water. Ammonia is an additional source of nitrogen as a nutrient which may contribute to
the expanded growth of undesirable algae and other forms of plant growth that overload the
natural system and cause pollution.
APPLICATION
Drinking, surface, and saline waters; domestic and industrial wastes.
RANGE
0 to 5.0 ppm Ammonia Nitrogen
METHOD
Ammonia forms a colored complex with Nessler’s Reagent in proportion to the amount of
ammonia present in the sample. Rochelle salt is added to prevent precipitation of calcium or
magnesium in undistilled samples.
HANDLING & PRESERVATION
Preservation is accomplished by the addition of 2 mL of concentrated H2SO4at 4°C.
INTERFERENCES
Sample turbidity and color may interfere. Turbidity may be removed by a filtration procedure.
Color interference may be eliminated by adjusting the instrument to 100%T with a sample blank.

4
ANALYSIS
PROCEDURE
1. Fill the Water Sample Collecting Bottle
(0688) with sample water.
2. Press and hold until colorimeter
turns on.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
3. Press ENTER to select Testing Menu.All Tests
008 Ammonia-N HR
12:00:00 001/500
4. Scroll to and select 008 Ammonia-N HR
from menu.
008 Ammonia-N HR
Scan Blank
Scan Sample
12:00:00 001/500
5. Rinse a clean tube (0290) with sample
water. Fill to the 10 mL line with sample.
Cap and wipe dry.
6. Insert tube into chamber, close lid and
select Scan Blank.

5
7. Remove tube from colorimeter. Add 8
drops of Ammonia Nitrogen Reagent #1
(V-4797). Cap and mix.
8. Use 1.0 mL pipet (0354) to add 1.0 mL
of *Ammonia Nitrogen Reagent #2
(V-4798).
9. Cap and invert to mix. Wait 5 minutes for
full color development. Wipe tube dry. 05:00
10. Insert tube into chamber. Close lid.
Select Scan Sample. Record result as
Ammonia-nitrogen.
008 Ammonia-N HR
0.99 ppm
Scan Blank
Scan Sample
12:00:00 001/500
NOTE: The meter will remember the last scanned blank reading. It is not necessary to scan a blank each time
the test is performed. To use the previous blank reading, instead of scanning a new one, scroll to Scan Sample
and proceed. For the most accurate results, the meter should be blanked before each test and the same tube
should be used for the blank and the reacted sample.
NOTE: For the best possible results, carry a reagent blank through the procedure. After scanning the
blank, perform the test procedure on clear, colorless, distilled or deioized water. Subtract results of regent
blank from all subsequent test results.
CALCULATIONS
To express results as Unionized Ammonia (NH3):
Unionized Ammonia (NH3) = ppm Ammonia Nitrogen (NH3-N) x 1.2
To express results as Ionized Ammonia (NH4+):
Ionized Ammonia (NH4+) = ppm Ammonia Nitrogen (NH3-N) x 1.3
Ammonia in water occurs in two forms: toxic unionized ammonia (NH3) and the relatively non-
toxic ionized form, ammonium ion (NH4+). This test method
measures both forms as ammonia-nitrogen (NH4-N) to give the total ammonia-nitrogen
concentration in water. The actual proportion of each compound depends on temperature,
salinity, and pH. A greater concentration of unionized ammonia is present when the pH value and
salinity increase.

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1. Consult the table below to find the percentage that corresponds to the temperature, pH and
salinity of the sample.
2. To express the test result as ppm Unionized Ammonia Nitrogen (NH3-N), multiply the total
ammonia-nitrogen test result by the percentage from the table.
3. To express the test result as ppm Ionized Ammonia Nitrogen (NH4+-N), subtract the
unionized ammonia-nitrogen determined in Step 2 from the total ammonia nitrogen.
10°C 15°C 20°C 25°C
pH FW1 SW2 FW SW FW SW FW SW
7.0 0.19 0.27 0.40 0.55
7.1 0.23 0.34 0.50 0.70
7.2 0.29 0.43 0.63 0.88
7.3 0.37 0.54 0.79 1.10
7.4 0.47 0.68 0.99 1.38
7.5 0.59 0.459 0.85 0.665 1.24 0.963 1.73 1.39
7.6 0.74 0.577 1.07 0.836 1.56 1.21 2.17 1.75
7.7 0.92 0.726 1.35 1.05 1.96 1.52 2.72 2.19
7.8 1.16 0.912 1.69 1.32 2.45 1.90 3.39 2.74
7.9 1.46 1.15 2.12 1.66 3.06 2.39 4.24 3.43
8.0 1.83 1.44 2.65 2.07 3.83 2.98 5.28 4.28
8.1 2.29 1.80 3.32 2.60 4.77 3.73 6.55 5.32
8.2 2.86 2.26 4.14 3.25 5.94 4.65 8.11 6.61
8.3 3.58 2.83 5.16 4.06 7.36 5.78 10.00 8.18
8.4 4.46 3.54 6.41 5.05 9.09 7.17 12.27 10.10
8.5 5.55 4.41 7.98 6.28 11.18 8.87 14.97 12.40
FOR EXAMPLE:
A fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as total Ammonia-
Nitrogen.
1. The percentage from the table is 11.18% (or 0.1118).
2. 1 ppm total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized
Ammonia-Nitrogen
3. Total Ammonia-Nitrogen 1.0000 ppm
Unionized Ammonia-Nitrogen – 0.1118 ppm
Ionized Ammonia-Nitrogen = 0.8882 ppm
CALIBRATION
STANDARDS
The meter should be calibrated with ammonia nitrogen standards. The calibration should be
done with a distilled or deionized water blank and one ammonia nitrogen standard of known
concentration. The concentration of the calibration standard should be similar to the expected
concentration of the sample that will be tested.
CALIBRATION PROCEDURE
1. Press and briefly hold to turn the
meter on. The LaMotte logo screen will
appear for about 3 seconds and the Main
Menu will appear.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500

7
2. Press ENTER to select Testing Menu.All Tests
008 Ammonia-N HR
12:00:00 001/500
3. Scroll to and select 008 Ammonia-N HR
from menu.
008 Ammonia-N HR
Scan Blank
Scan Sample
12:00:00 001/500
4. Rinse a clean tube (0290) three times
with the ammonia nitrogen standard.
Fill to the 10 mL line with the ammonia
nitrogen standard. Cap the tube. Dry the
tube with a lint-free cloth.
5. Insert tube into chamber, close lid and
select Scan Blank.
6. Remove tube from colorimeter. Add 8
drops of Ammonia Nitrogen Reagent #1
(V-4797). Cap and mix.

8
7. Use 1.0 mL pipet (0354) to add 1.0 mL
of *Ammonia Nitrogen Reagent #2
(V-4798).
8. Cap and invert to mix. Wait 5 minutes for
full color development. Wipe tube dry. 05:00
9. Insert tube into chamber. Close lid.
Select Scan Sample.
008 Ammonia-N HR
0.99 ppm
Scan Blank
Scan Sample
12:00:00 001/500
10. Press to scroll to Calibrate.008 Ammonia-N HR
0.99 ppm
%T/Abs
Calibrate
12:00:00 001/500
11. Press ENTER to select Calibrate. A
reverse font (light background with dark
characters) will appear to indicate that
the reading can be adjusted.
008 Ammonia-N HR
0.99 ppm
∧, ∨=Edit, ENTER=Save
∧ +ENTER=Default
12:00:00 001/500
12. Press or to adjust the
value shown to the concentration of the
prepared standard, 1.00 in this example.
NOTE: A maximum adjustment of 25% is
possible.
008 Ammonia-N HR
1.00 ppm
∧, ∨=Edit, ENTER=Save
∧ +ENTER=Default
12:00:00 001/500

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13. Press ENTER to save the value.
To leave the Calibration procedure
without saving the adjustment, press
EXIT .
Press and ENTER at any time to
return to the default value.
The calibration has now been
standardized and can be used for
testing. Scroll to Scan Blank and begin
testing.
008 Ammonia-N HR
1.00 ppm
%T/Abs
Calibrate
12:00:00 001/500
SET UP
SETTING THE CLOCK
Setting the clock allows the correct time and date stamp to be stored with each reading in the
data logger.
1. From the Editing Menu, press or
to scroll to Set Clock.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
2. Press ENTER to select Set Clock. The
year is displayed. Press or
to scroll to the appropriate character.
Press ENTER to select the character. The
month, day, hour, format hour, minute,
second, AM/PM will be dislpayed. Repeat
for each.
Set Time
Year: 2000
12:00:00 001/500
3. Press ENTER to select the final
character. The time and date will be
saved and the meter will return to the
Editing Menu.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
LOGGING DATA
The default setting for the data logger is enabled. The meter will log the last 500 data points. The
counter in the center bottom of the display will show how many data points have been logged.
The display will show 500+ when the data logger has exceeded 500 points and the data points
are being overwritten.

10
1. From the Editing Menu, press or
to scroll to Logging.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
2. Press ENTER to select Logging. Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
3. Press or to scroll to
desired function.
Logging
Display Test Log
Logging Enabled
Logging Disabled
Erase Log
12:00:00 001/500
4. Press ENTER . The screen will display
Storing... for about 1 second and return
to the Editing Menu.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
FACTORY SETUP
The Factory Setup menu is used in manufacturing of the colorimeter. This menu is not for use by
the operator in the field.
SETTING POWER SAVE
The power saving Auto Shutoff feature will turn the meter off when a button has not been pushed
for a set amount of time. The default setting is disabled. To change the setting:
1. From the Editing Menu, press or
to scroll to Set Power Save.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500

11
2. Press ENTER to select Set Power Save. Set Power Save
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
3. Press or to scroll to
desired function.
Set Power Save
Disable
5 Minutes
15 Minutes
30 Minutes
12:00:00 001/500
4. Press ENTER . The screen will display
Storing... for about 1 second and the
meter will retun to the Editing Menu.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
SETTING THE BACKLIGHT TIME
The backlight illuminates the display for enhanced viewing. The default setting is 10 seconds. If
Button Control is chosen the backlight button on the key pad will act as an on/off switch and the
backlight will remain on or off when the meter is being used. When one of the other settings – 10,
20 or 30 seconds – is chosen, the display will be illuminated for the specified amount of time
after any button is pressed.
NOTE: The backlight feature uses a significant amount of power. The longer the backlight is on, the more
frequently the battery will have to be charged if the USB/Wall Adapter is not being used.
1. From the Editing Menu, press or
to scroll to Backlight Time.
Editing Menu
Logging
Factory Setup
Set Power Save
Set Backlight Time
12:00:00 001/500
2. Press ENTER to select Set Backlight
Time.
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500

12
3. Press or to scroll to
desired option.
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
4. Press ENTER . The screen will display
Storing... for about 1 second and the
meter will return to the Editing Menu.
Editing Menu
Logging
Factory Setup
Set Power Save
Set Backlight Time
12:00:00 001/500
SELECTING A LANGUAGE
There are eight languages available: English, Spanish, French, Portuguese, Italian, Chinese,
Japanese, and Turkish.
1. From the Editing Menu, press or
to scroll to Select Language.
Editing Menu
Factory Setup
Set Power Save
Set Backlight Time
Select Language
12:00:00 001/500
2. Press ENTER to select Select Language. Select Language
English
Spanish
French
Portugese
12:00:00 001/500
3. Press or to scroll to
desired language.
Select Language
English
Spanish
French
Portugese
12:00:00 001/500

13
4. Press ENTER . The screen will display
Storing... for about 1 second and the
meter will return to the Editing Menu.
Editing Menu
Set Power Save
Set Backlight Time
Bluetooth Menu
Select Language
12:00:00 001/500
NOTE: If meter unintentionally switches to another language, use the procedure above to reset the meter to
the desired language. For example, to reset the meter to English:
Turn meter on.
1. Press one time. Press ENTER .
2. Press five times. Press ENTER .
3. Press ENTER .
LOOPING MENUS
Long menus, such as the Tsting Menu, incorporate a looping feature which allows the user to
quickly reach the last choice in the menu from the first choice. In a looping menu the last choices
in the menu are above the first choice and scrolling upward moves through the menu in reverse
order. Scrolling downward moves through the menu from first choice to last but the menu starts
over following the last choice. So all menu choices can be reached by scrolling in either direction.
The diagrams below demonstrate a looping menu.
AND SO ON AND SO ON AND SO ON
: : : : : : : : :
: : : : : : : : :
THIRD TO LAST LAST CHOICE LAST CHOICE
SECOND TO LAST AND SO ON AND SO ON
LAST CHOICE : : : : : :
TESTING MENU TESTING MENU TESTING MENU
FIRST CHOICE : : : : : :
SECOND CHOICE THIRD TO LAST THIRD TO LAST
THIRD CHOICE SECOND TO LAST SECOND TO LAST
ANOTHER LAST CHOICE LAST CHOICE
12:00:00 001/500 12:00:00 001/500 12:00:00 001/500
AND ANOTHER FIRST CHOICE FIRST CHOICE
AND SO ON SECOND CHOICE SECOND CHOICE
: : : THIRD CHOICE THIRD CHOICE
: : : ANOTHER ANOTHER
LAST CHOICE AND ANOTHER AND ANOTHER
The feature called Looping Menu can be turned on and off in the Editing Menu. The default
setting is ON.
COMPUTER CONNECTION
OUTPUT
USB
COMPUTER CONNECTION
USB Type A, USB mini B, Order Cable Code 1720-01.
WATERLINK CONNECT 2
The meter may be interfaced with any Windows-based 64-bit computer by using the LaMotte
WaterLink Connect 2 program and a USB cable. The program will store test information and
results in a local database and allow for exporting this data to a comma separated value (CSV)

14
file. The meter will send the following data: name of test, sample value, sample units, time/date
stamp, and meter name. To download WaterLink Connect 2 go to http:// softwarecenter.lamotte.
com/. Select “WaterLink Connect 2 FREE Download”.
BATTERY
BATTERY/AC OPERATION
The meter may be operated on battery power using a USB wall adapter or USB computer
connection. If using the meter as a bench top unit, use the wall adapter if possible to extend the
battery life. The meter will remain on when the USB adapter is used.
To charge the lithium ion battery with the wall adapter, plug the smaller end of the USB cable
(USB mini B connector) into the meter and the larger end of the USB cable (USB type A
connector) into the wall adapter. Plug the wall adapter into an AC outlet. Reinsert the rubber USB
port plug after charging. Failure to insert the USB port plug when the meter is not connected to a
computer by USB or actively charging by USB could result in damage to internal components.
To charge the battery from a computer, plug the smaller end of the USB cable (USB mini B
connector) into the meter and the larger end of the USB cable (USB Type A connector) into a USB
port on a computer. Reinsert the USB port plug after charging. Failure to insert the USB port plug
when the meter is not connected to a computer by USB or actively charging by USB could result
in damage to internal components.
The battery icon will show no bars and flash when the unit first turns on. Then the indicator will
indicate the battery status by showing 0, 1, 2, 3, or 4 bars. It will take 6 hours to fully charge a
low battery. The battery icon will flash when the battery is charging. The battery icon will show
four bars and stop flashing when it is fully charged. The charging circuit will automatically switch
to a float charge when the battery is fully charged. The charger may remain connected. Some
computers will NOT supply power to their USB ports during standby operation. The wall adapter
will charge the unit continuously. Storing the meter above ambient room temperature will
decrease the battery charge more quickly than storage at room temperature. If the meter does
not turn on, it means that the battery is at a very low charge. Charging the battery with the wall
adapter in this state may take up to 10 hours. At low temperatures, approaching 0 °C, the battery
will charge more slowly. It will not charge at all below 0 °C.
The battery icon will show no bars and continuously flash if the battery is getting low but the
unit will still operate normally. A “Low Battery” message on the status bar of the display will
replace the time when the battery voltage is too low for proper operation and accuracy may be
degraded. A “Shutdown Low Batt” message on the display will appear for a few seconds before
the power is switched off when the battery is too low to operate the unit. When the battery icon
simultaneously flashes bars 1 and 2 followed by bars 3 and 4, it is an indication that the battery is
damaged and technical support should be contacted.
To extend the life of the battery:
• Shut down the unit with the power switch when not taking measurements or use the power
save option to have the unit automatically turn off after 5 minutes.
• Store the unit at a moderate temperature.
• Fully charge the battery before storing the unit for extended periods of time.
• Fully charge the battery at least once per year. Failure to do so may result in a permanently
drained battery.
• Limit backlight use. The unit consumes three times the normal power when the backlight
is on. Set the backlight time option to 10 seconds or select “Button Control” and keep the
backlight off.
BATTERY REPLACEMENT
The lithium ion battery used in this unit should last for many years with normal use. When it
no longer powers the unit long enough to meet testing requirements it should be replaced.
Contact LaMotte Company by phone [phone]) or email ([e-mail]) for more
information.
MAINTENANCE
CLEANING
Clean the exterior housing with a damp, lint-free cloth. Do not allow water to enter the light
chamber or any other parts of the meter. To clean the light chamber and optics area, point a can
of compressed air into the light chamber and blow the pressurized air into the light chamber.
Use a cotton swab dampened with Windex®window cleaner to gently swab the interior of the
chamber. Do not use alcohol; it will leave a thin residue over the optics when dry.

15
RETURNS
Should it be necessary to return the meter, pack the meter carefully in a suitable container with
adequate packing material. A return authorization number must be obtained from LaMotte
Company by calling 800-344-3100 (US only) or 410-778-3100, faxing 410-778-6394, or
emailing [email protected]. Often a problem can be resolved over the phone or by email. If a
return of the meter is necessary, attach a letter with the return authorization number, meter
serial number, a brief description of problem and contact information including phone and FAX
numbers to the shipping carton.
METER DISPOSAL
Waste Electrical and Electronic Equipment (WEEE)
Natural resources were used in the production of this equipment. This equipment may contain
materials that are hazardous to health and the environment. To avoid harm to the environment
and natural resources, the use of appropriate take-back systems is recommended. The crossed
out wheeled bin symbol on the meter encourages the use of these systems when disposing of
this equipment.
Take-back systems will allow the materials to be reused or recycled in a way that will
not harm the environment. For more information on approved collection, reuse, and
recycling systems contact local or regional waste administration or recycling services.
GENERAL OPERATING INFORMATION
OVERVIEW
The meter is a portable, microprocessor controlled, direct reading colorimeter. It has a graphical
liquid crystal display and 6 button keypad. These allow the user to select options from the menu
driven software, to directly read test results or to review stored results of previous tests in the
data logger. The menus can be displayed in eight different languages.
The LaMotte tests are precalibrated for LaMotte reagent systems.The colorimeter displays the
result of these tests directly in units of concentration.
The optics feature a colored LED. The LED has a corresponding silicon photoiode with an
integrated interference filter. The interference filter selects a narrow band of light from the
corresponding LED for the colorimetric measurements.
A USB wall adapter, USB computer connection or lithium battery powers the colorimeter.
GENERAL OPERATING INFORMATION
The operation of the colorimeter is controlled by the menu driven software and user interface.
A menu is a list of choices. This allows a selection of various tasks for the colorieter to perform,
such as scan blank and scan sample. The keypad is used to make menu selections that are
viewed on the display.
THE KEYPAD
The keypad has 6 buttons which are used to perform specific tasks.
This button will scroll up through a list of menu selections.
ENTER
The button is used to select choices in a menu viewed on the
display.
This button controls the backlight on the display.
This button will scroll down through a list of menu selections.
EXIT This button exits to the previous menu.
This button turns the meter on or off.
THE DISPLAY AND MENUS
The display allows menu selections to be viewed and selected. These selections instruct the
colorimeter to perform specific tasks. The menus are viewed in the display using two general
formats that are followed from one menu to the next. Each menu is a list of choices or selections.
The display has a header line at the top and a footer line at the bottom. The header displays the
title of the current menu. The footer line displays the time and the date, the data logger status
and the battery status. The menu selection window is in the middle of the display between the
header and the footer.
The menu selection window displays information in two general formats. In the first format
only menu selections are displayed. Up to 4 lines of menu selections may be displayed. If more
selections are available they can be viewed by pressing the arrow buttons to scroll
the other menu selections into the menu selection window. Think of the menu selections as a
EX I T
ENTER

16
vertical list in the display that moves up or down each time an arrow button is
pressed. Some menus in the colorimeter are looping menus. The top and bottom menu choices
are connected in a loop. Scrolling down past the bottom of the menu will lead to the top of the
menu. Scrolling up past the top of the menu will lead to the bottom of the menu.
Header Menu Title
Main Window Selection First Choice
Second Choice
Third Choice
Another
Footer 12:00:00 001/500 P
And Another
And So On
A light bar will indicate the menu choice. As the menu is scrolled through, the light bar will
highlight different menu choices. Pressing the ENTER button will select the menu choice that is
indicated by the light bar.
In the second format the menu choice window takes advantage of the graphical capabilities
of the display. Large format graphic information, such as test results or error messages or the
LaMotte logo is displayed. The top two lines of the display are used to display information in a
large, easy to read format. The menus work in the same way as previously described but two lines
of the menu are visible at the bottom of the display.
Header Menu Title
Message or Result Window Result or
Message
Another
Main Window Selection And Another
Footer 12:00:00 001/500
And So On
Last Choice
As described previously, the EXIT button allows an exit or escape from the current menu and
a return to the previous menu. This allows a rapid exit from an inner menu to the main menu by
repeatedly pushing the EXIT button. Pushing at any time will turn the colorimeter off.
The display may show the following messages:
Battery Status
More choices are available and can be viewed by scrolling up and/or down through the display.
Header Identifies the current menu and information on units and reagent systems if applicable.
Footer In the data logging mode the number of the data point is displayed and the total number
of data points in the memory will be shown. The footer also shows current time and battery
status
TUBES AND CHAMBERS
The colorimeter uses one type of tube (Code 0290) for all test factors.
The handling of the tubes is of utmost importance. Tubes must be clean and free from lint,
fingerprints, dried spills and significant scratches, especially the central zone between the
bottom and the sample line.

17
Scratches, fingerprints and water droplets on the tube can cause stray light interference leading
to inaccurate results. Tubes that have been scratched in the light zone through excessive use
should be discarded and replaced with new ones.
Tubes should always be washed on the inside and outside with mild detergent prior to use to
remove dirt or fingerprints. The tubes should be allowed to air-dry in an inverted position to
prevent dust from entering the tubes. Dry tubes should be stored with the caps on to prevent
contamination.
After a tube has been filled and capped, it should be held by the cap and the outside surface
should be wiped with a clean, lint-free absorbent cloth until it is dry and smudge-free. Handling
the tube only by the cap will avoid problems from fingerprints. Always set the clean tube aside
on a clean surface that will not contaminate the tube. It is imperative that the tubes and light
chamber be clean and dry. The outside of the tubes should be dried with a clean, lint-free cloth or
disposable wipe before they are placed in the meter chamber.
Tubes should be emptied and cleaned as soon as possible after reading a sample to prevent
deposition of particulates on the inside of the tubes.
Variability in the geometry of the glassware and technique is the predominate cause of variability
in results. Slight variations in wall thickness and the diameter of the tubes may lead to slight
variations in the test results. To eliminate this error the tubes should be placed in the chamber
with the same orientation each time.
Chambers which have been scratched through excessive use should be discarded and replaced
with a new one.
SAMPLE DILUTION TECHNIQUES
If a test result is out of the range of the meter, it must be diluted. The test should then be
repeated on the diluted sample. The following table gives quick reference guidelines for dilutions
of various proportions.
Amount of Sample Deionized Water to Bring Final Volume to 10 mL Multiplication Factor
10 mL 0 mL 1
5 mL 5 mL 2
2.5 mL 7.5 mL 4
1 mL 9 mL 10
0.5 mL 9.5 mL 20
All dilutions are based on a final volume of 10 mL, so several dilutions will require small volumes
of the water sample. Graduated pipets should be used for all dilutions. If volumetric glassware is
not available, dilutions can be made with the colorimeter tube. Fill the tube to the 10 mL line with
the sample and then transfer it to another container. Add 10 mL volumes of deionized water to
the container and mix. Transfer 10 mL of the diluted sample to the colorimeter tube and follow
the test procedure. Repeat the dilution and testing procedures until the result falls within the
range of the calibration. Multiply the test result by the dilution factor. For example, if 10 mL of the
sample water is diluted with three 10 mL volumes of deionized water, the dilution factor is four.
The test result of the diluted sample should be multiplied by four.
GENERAL INFORMATION
PACKAGING AND DELIVERY
Experienced packaging personnel at LaMotte Company assure adequate protection against
normal hazards encountered in transportation of shipments.
After the product leaves LaMotte Company, all responsibility for safe delivery is assured by the
transportation company. Damage claims must be filed immediately with the transportation
company to receive compensation for damaged goods.
GENERAL PRECAUTIONS
READ THE INSTRUCTION MANUAL BEFORE ATTEMPTING TO SET UP OR OPERATE THE METER.
Failure to do so could result in personal injury or damage to the meter. The meter should not be
used or stored in a wet or corrosive environment. Care should be taken to prevent water from wet
tubes from entering the meter chamber.
NEVER PUT WET TUBES IN THE METER.
SAFETY PRECAUTIONS
Read the labels on all LaMotte reagent containers prior to use. Some containers include
precautionary notices and first aid information. Certain reagents are considered hazardous

18
substances and are designated with a * in the instruction manual. Safety Data Sheets (SDS) can
be found at www.lamotte.com. Read the SDS before using these reagents. Additional emergency
information for all LaMotte reagents is available 24 hours a day from the National Poison Control
Center 1-800-222-1222 or by contacting the 24 hour emergency line for ChemTel 1-800-255-
3924 (USA, Canada, Puerto Rico); locations outside the North American Continent 813-248-
0585 (call collect). Be prepared to supply the name and four-digit LaMotte code number found
on the container label or at the top of the SDS or in the contents list of the procedure. LaMotte
reagents are registered with a computerized poison control information system available to all
local poison control centers.
Keep equipment and reagent chemicals out of the reach of young children.
Ensure that the protection provided by this equipment is not impaired. Do not intall or use this
equipment in a manner that is not indicated in this manual.
LIMITS OF LIABILITY
Under no circumstances shall LaMotte Company be liable for loss of life, property, profits, or other
damages incurred through the use or misuse of its products.
SPECIFICATIONS & RANGES
INSTRUMENT TYPE: Colorimeter
Readout 160 x 100 backlit LCD, 20 x 6 line graphical display
Wavelengths 428 nm
Wavelength Accuracy ±2% FS
Readable Resolution Determined by reagent system
Wavelength Bandwidth 10 nm typical
Photometric Range –2 to +2 AU
Photometric Precision ± 0.001 AU at 1.0 AU
Photometric Accuracy ±0.005 AU at 1.0 AU
Sample Chamber Accepts 25 mm diameter flat-bottomed test tubes
Light Sources 1 LEDs
Detectors 1 silicon photodiode
Modes Pre-programmed tests, absorbance, %T
Pre-Programmed Tests YES, with automatic wavelength selection
Languages English, Spanish, French, Portuguese, Italian, Chinese, Japanese, Turkish
Temperature Operation: 0-50 °C; Storage: -40-60 °C
Operation Humidity Range 0-90 % RH, non-condensing
USB Port Mini B
Power Requirements USB wall adapter, USB computer connection or lithium ion rechargeable
battery
Battery Charge Life: Approximately 380 tests with backlight on to 1000 tests with
backlight off. Battery Life: Approximately 500 charges.
Electrical Rating Rated voltage (5V), Rated power of input current (1.0A) at mini-USB input
port
Data Logger 500 test results
Waterproof IP67 with USB port plug in place
Dimensions (LxWxH) 3.5 x 7.5 x 2.5 inches, 8.84 x 19.05 x 6.35 cm
Weight 13 oz, 362 g (meter only)
CE COMPLIANCE
The colorimeter has been independently tested and has earned the European CE Mark of
compliance for electromagnetic compatibility and safety. To view certificates of compliance, go to
the LaMotte website at www.lamotte.com.

19
IP67 Certification
The meets IP67 standards for protection against dust and immersion only when the USB port
plug is in place. Documentation is available at www.lamotte.com.
WARRANTY
LaMotte Company warrants this instrument to be free of defects in parts and workmanship for 2
years from the date of shipment. Keep the proof of purchase for warranty verification. If it should
become necessary to return the instrument during or the warranty period, contact our Technical
Service Department at 1-800-344-3100 or 1-410-778-3100, ext. 3 or softwaresupport@
lamotte.com for a return authorization number or visit www.lamotte.com for troubleshooting
help. The sender is responsible for shipping charges, freight, insurance, and proper packaging to
prevent damage in transit. This warranty does not apply to defects resulting from action of the
user such as misuse, improper wiring, operation outside of specification, improper maintenance
or repair, or unauthorized modification. LaMotte Company specifically disclaims any implied
warranties or merchantability or fitness for a specific purpose and will not be liable for any direct,
indirect, incidental, or consequential damages. LaMotte Company’s total liability is limited to
repair or replacement of the product with a new or refurbished meter as determined by LaMotte
Company. The warranty set forth above is inclusive and no other warranty, whether written or oral,
is expressed or implied.
TROUBLESHOOTING
ERROR MESSAGES - Over Range
f the message OVERRANGE is displayed when scanning a sample, the sample may be over range
or under range. If the sample is over range, the sample should be diluted and tested again (see
Sample Dilution Techniques and Volumetric Measurements).
If overrange is displayed, scroll to Calibrate
and press ENTER to view the result. The
concentration is only an approximation. Press
EXIT and continue testing.
Note: If EXIT is not pressed the meter will
calibrate on the over range result.
Note: Over range results will also be displayed in
the test log.
008 Ammonia-N HR
Overrange
<Enter> continue
Scan Blank
Scan Sample
12:00:00 001/500
CALIBRATION
As with all pre-calibrated meters, it is highly recommended, even if not required by regulations,
that the user periodically verify the performance of the meter by running standards with a
predetermined concentration. Results outside of specification are an indication that the meter
needs to be adjusted. This can be done following the Calibration procedure.
STRAY LIGHT
The colorimeter should have no problems with stray light. Make sure that the sample
compartment lid is always fully closed.
TROUBLESHOOTING GUIDE
PROBLEM REASON SOLUTION
Flashing Low battery. Readings are
reliable.
Charge battery or use USB wall/computer
adapter.
“Low Battery” Battery voltage is very low.
Readings are not reliable.
Charge battery or use USB wall/computer
adapter.
“Shut Down Low Batt” Shut
Down
Battery is too low to operate
the unit.
Charge battery or use USB wall/computer
adapter.
“Overrange” Sample is outside of
acceptable range.
Dilute sample and test again.
Unusually large negative
or positive readings when
performing calibration
Incorrect standards used to
calibrate meter.
Use fresh 0.0 standard in clean tube.
Reset meter to factory default settings.
Recalibrate meter.

802 Washington Ave • Chestertown • Maryland • 21620 • USA
[phone] • 800-344-3100
www.lamotte.com
LaMotte and WaterLink, are ® registered trademarks of LaMotte Company | © 2023 LaMotte Company, All Rights Reserved.
3241-MN | 10.10.2023